Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84847.t1 | XP_013467004.1 | 82.5 | 360 | 16 | 5 | 1 | 316 | 1 | 357 | 3.10E-129 | 471.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84847.t1 | A0A072VG82 | 82.5 | 360 | 16 | 5 | 1 | 316 | 1 | 357 | 2.3e-129 | 471.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene84847 | 0.818787 | 1.63E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene84847 | 0.806538 | 7.84E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene84847 | 0.860889 | 1.51E-63 | -1.69E-46 |
| MS.gene049808 | MS.gene84847 | 0.815914 | 7.24E-52 | -1.69E-46 |
| MS.gene049821 | MS.gene84847 | 0.814162 | 1.77E-51 | -1.69E-46 |
| MS.gene050237 | MS.gene84847 | 0.829802 | 4.20E-55 | -1.69E-46 |
| MS.gene050241 | MS.gene84847 | 0.800555 | 1.36E-48 | -1.69E-46 |
| MS.gene050273 | MS.gene84847 | 0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene84847 | 0.83632 | 1.00E-56 | -1.69E-46 |
| MS.gene051198 | MS.gene84847 | 0.832482 | 9.22E-56 | -1.69E-46 |
| MS.gene051279 | MS.gene84847 | 0.843988 | 9.98E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene84847 | 0.803941 | 2.74E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene84847 | 0.843835 | 1.10E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene84847 | 0.826956 | 2.04E-54 | -1.69E-46 |
| MS.gene052239 | MS.gene84847 | -0.801049 | 1.08E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene84847 | 0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene052516 | MS.gene84847 | 0.843719 | 1.18E-58 | -1.69E-46 |
| MS.gene052519 | MS.gene84847 | 0.829687 | 4.48E-55 | -1.69E-46 |
| MS.gene052561 | MS.gene84847 | 0.800561 | 1.36E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene84847 | 0.823874 | 1.10E-53 | -1.69E-46 |
| MS.gene052773 | MS.gene84847 | 0.808182 | 3.51E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene84847 | 0.814447 | 1.53E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene84847 | 0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene84847 | 0.831401 | 1.70E-55 | -1.69E-46 |
| MS.gene053166 | MS.gene84847 | 0.812966 | 3.25E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene84847 | 0.824223 | 9.07E-54 | -1.69E-46 |
| MS.gene053599 | MS.gene84847 | 0.830357 | 3.07E-55 | -1.69E-46 |
| MS.gene05364 | MS.gene84847 | 0.819445 | 1.16E-52 | -1.69E-46 |
| MS.gene053678 | MS.gene84847 | 0.81875 | 1.67E-52 | -1.69E-46 |
| MS.gene053894 | MS.gene84847 | 0.802243 | 6.15E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene84847 | 0.806287 | 8.85E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene84847 | 0.803815 | 2.91E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene84847 | 0.809594 | 1.75E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene84847 | 0.814929 | 1.20E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene84847 | 0.84088 | 6.66E-58 | -1.69E-46 |
| MS.gene054775 | MS.gene84847 | 0.822526 | 2.26E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene84847 | 0.813614 | 2.34E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene84847 | 0.810547 | 1.09E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene84847 | 0.812179 | 4.83E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene84847 | 0.816591 | 5.11E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene84847 | 0.815703 | 8.07E-52 | -1.69E-46 |
| MS.gene055512 | MS.gene84847 | 0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene84847 | 0.803851 | 2.86E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene84847 | 0.801512 | 8.69E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene84847 | 0.811678 | 6.21E-51 | -1.69E-46 |
| MS.gene056173 | MS.gene84847 | 0.805236 | 1.47E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene84847 | 0.802437 | 5.61E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene84847 | 0.820329 | 7.27E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene84847 | 0.814354 | 1.61E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene84847 | 0.81112 | 8.21E-51 | -1.69E-46 |
| MS.gene057021 | MS.gene84847 | 0.817899 | 2.60E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene84847 | 0.811073 | 8.40E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene84847 | 0.858716 | 6.81E-63 | -1.69E-46 |
| MS.gene057414 | MS.gene84847 | 0.809393 | 1.93E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene84847 | 0.826371 | 2.82E-54 | -1.69E-46 |
| MS.gene058009 | MS.gene84847 | 0.816657 | 4.94E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene84847 | 0.805694 | 1.18E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene84847 | 0.825326 | 4.98E-54 | -1.69E-46 |
| MS.gene058636 | MS.gene84847 | 0.80113 | 1.04E-48 | -1.69E-46 |
| MS.gene058783 | MS.gene84847 | 0.801698 | 7.96E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene84847 | 0.811542 | 6.65E-51 | -1.69E-46 |
| MS.gene059015 | MS.gene84847 | 0.813407 | 2.60E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene84847 | 0.802263 | 6.09E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene84847 | 0.811081 | 8.37E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene84847 | 0.809058 | 2.28E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene84847 | 0.824105 | 9.66E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene84847 | 0.807902 | 4.03E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene84847 | 0.82569 | 4.09E-54 | -1.69E-46 |
| MS.gene060603 | MS.gene84847 | 0.803906 | 2.79E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84847.t1 | MTR_1g041155 | 82.500 | 360 | 16 | 5 | 1 | 316 | 1 | 357 | 0.0 | 552 |
| MS.gene84847.t1 | MTR_1g076500 | 50.649 | 385 | 113 | 13 | 1 | 311 | 1 | 382 | 2.42e-103 | 308 |
| MS.gene84847.t1 | MTR_7g095960 | 38.814 | 371 | 150 | 11 | 4 | 316 | 5 | 356 | 6.40e-70 | 221 |
| MS.gene84847.t1 | MTR_1g070970 | 39.837 | 369 | 139 | 11 | 4 | 314 | 5 | 348 | 1.85e-68 | 217 |
| MS.gene84847.t1 | MTR_8g093440 | 41.606 | 137 | 73 | 1 | 119 | 248 | 27 | 163 | 4.77e-36 | 129 |
| MS.gene84847.t1 | MTR_8g093440 | 41.606 | 137 | 73 | 1 | 119 | 248 | 89 | 225 | 1.20e-35 | 130 |
| MS.gene84847.t1 | MTR_7g083080 | 49.167 | 120 | 55 | 2 | 119 | 232 | 137 | 256 | 2.34e-34 | 128 |
| MS.gene84847.t1 | MTR_4g132963 | 46.018 | 113 | 61 | 0 | 119 | 231 | 122 | 234 | 5.96e-34 | 125 |
| MS.gene84847.t1 | MTR_4g132940 | 46.018 | 113 | 61 | 0 | 119 | 231 | 122 | 234 | 5.96e-34 | 125 |
| MS.gene84847.t1 | MTR_4g132970 | 43.089 | 123 | 70 | 0 | 119 | 241 | 143 | 265 | 2.39e-33 | 125 |
| MS.gene84847.t1 | MTR_2g025520 | 48.214 | 112 | 58 | 0 | 120 | 231 | 142 | 253 | 2.76e-33 | 125 |
| MS.gene84847.t1 | MTR_2g013780 | 42.623 | 122 | 64 | 2 | 117 | 232 | 148 | 269 | 2.99e-32 | 122 |
| MS.gene84847.t1 | MTR_4g078310 | 41.176 | 119 | 61 | 2 | 119 | 229 | 126 | 243 | 2.72e-29 | 114 |
| MS.gene84847.t1 | MTR_4g088770 | 38.525 | 122 | 69 | 2 | 117 | 232 | 154 | 275 | 7.15e-29 | 113 |
| MS.gene84847.t1 | MTR_4g088760 | 38.525 | 122 | 69 | 2 | 117 | 232 | 207 | 328 | 1.64e-28 | 113 |
| MS.gene84847.t1 | MTR_2g013880 | 33.600 | 125 | 77 | 2 | 119 | 237 | 150 | 274 | 4.63e-26 | 105 |
| MS.gene84847.t1 | MTR_8g012240 | 36.691 | 139 | 81 | 2 | 101 | 232 | 120 | 258 | 6.03e-26 | 105 |
| MS.gene84847.t1 | MTR_5g008720 | 35.714 | 112 | 66 | 1 | 124 | 229 | 109 | 220 | 4.42e-23 | 96.3 |
| MS.gene84847.t1 | MTR_7g053310 | 36.667 | 120 | 70 | 2 | 120 | 233 | 128 | 247 | 4.64e-23 | 97.1 |
| MS.gene84847.t1 | MTR_1g103610 | 85.294 | 34 | 5 | 0 | 214 | 247 | 1 | 34 | 9.43e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84847.t1 | AT3G22970 | 44.000 | 375 | 139 | 10 | 4 | 316 | 5 | 370 | 5.52e-80 | 248 |
| MS.gene84847.t1 | AT4G14620 | 46.479 | 355 | 129 | 15 | 4 | 314 | 2 | 339 | 1.18e-78 | 243 |
| MS.gene84847.t1 | AT2G38820 | 39.936 | 313 | 130 | 8 | 4 | 266 | 5 | 309 | 3.47e-70 | 221 |
| MS.gene84847.t1 | AT3G22970 | 52.217 | 203 | 83 | 4 | 120 | 316 | 65 | 259 | 1.84e-55 | 181 |
| MS.gene84847.t1 | AT3G54550 | 37.269 | 271 | 117 | 9 | 5 | 231 | 6 | 267 | 5.03e-52 | 173 |
| MS.gene84847.t1 | AT3G07350 | 44.538 | 119 | 60 | 1 | 120 | 232 | 143 | 261 | 8.97e-34 | 125 |
| MS.gene84847.t1 | AT2G39650 | 44.348 | 115 | 58 | 1 | 124 | 232 | 60 | 174 | 1.88e-31 | 118 |
| MS.gene84847.t1 | AT2G39650 | 44.348 | 115 | 58 | 1 | 124 | 232 | 122 | 236 | 4.27e-31 | 118 |
| MS.gene84847.t1 | AT3G25240 | 39.568 | 139 | 75 | 2 | 103 | 232 | 104 | 242 | 2.68e-29 | 113 |
| MS.gene84847.t1 | AT4G32480 | 41.880 | 117 | 62 | 2 | 120 | 230 | 124 | 240 | 1.84e-28 | 111 |
| MS.gene84847.t1 | AT2G20670 | 40.708 | 113 | 61 | 1 | 124 | 230 | 130 | 242 | 2.82e-27 | 108 |
| MS.gene84847.t1 | AT1G62420 | 38.261 | 115 | 68 | 1 | 120 | 231 | 116 | 230 | 4.10e-26 | 105 |
| MS.gene84847.t1 | AT1G77160 | 35.625 | 160 | 80 | 3 | 119 | 260 | 32 | 186 | 1.06e-25 | 102 |
| MS.gene84847.t1 | AT1G12030 | 38.938 | 113 | 68 | 1 | 120 | 231 | 118 | 230 | 3.32e-25 | 102 |
| MS.gene84847.t1 | AT1G77160 | 35.625 | 160 | 80 | 3 | 119 | 260 | 87 | 241 | 3.36e-25 | 102 |
| MS.gene84847.t1 | AT1G77145 | 36.765 | 136 | 68 | 2 | 119 | 236 | 82 | 217 | 7.27e-25 | 101 |
| MS.gene84847.t1 | AT1G77145 | 39.216 | 102 | 52 | 1 | 145 | 236 | 59 | 160 | 8.11e-21 | 89.0 |
| MS.gene84847.t1 | AT2G38820 | 32.105 | 190 | 75 | 6 | 4 | 143 | 5 | 190 | 1.18e-19 | 85.9 |
Find 76 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGTTTGCTTGGCGAAAA+TGG | 0.121680 | 1.2:-25408259 | MS.gene84847:CDS |
| CATCCATTTGCAAATCAAAA+TGG | 0.188560 | 1.2:-25407847 | MS.gene84847:CDS |
| TTCAACCTCCACTTCGAAAC+TGG | 0.321490 | 1.2:+25408366 | None:intergenic |
| TCGGAGGTTGAAAGTGATTC+TGG | 0.325133 | 1.2:-25407389 | MS.gene84847:CDS |
| GCCGCATCGGAGACTTGCTT+TGG | 0.348894 | 1.2:+25407434 | None:intergenic |
| GAGTCCAGCGTGGCAGTTAC+CGG | 0.378357 | 1.2:-25407288 | MS.gene84847:CDS |
| ATCCATTTGCAAATCAAAAT+GGG | 0.412073 | 1.2:-25407846 | MS.gene84847:CDS |
| CTCCGATGCGGCGGCGATGC+AGG | 0.413399 | 1.2:-25407423 | MS.gene84847:CDS |
| TTCTCTGAATCTATGTCAAT+CGG | 0.419113 | 1.2:+25408417 | None:intergenic |
| TCTTTAGTGGTATTGTTCTT+CGG | 0.421271 | 1.2:+25408315 | None:intergenic |
| ATTCCTCTTTCACCACTCTT+CGG | 0.422133 | 1.2:+25407257 | None:intergenic |
| GTGGTATTGTTCTTCGGAAA+AGG | 0.429831 | 1.2:+25408321 | None:intergenic |
| TGAAAGTGATTCTGGTGAAT+TGG | 0.430639 | 1.2:-25407381 | MS.gene84847:CDS |
| TCCACCACCACCATCTTTAG+TGG | 0.439948 | 1.2:+25408302 | None:intergenic |
| TTTCGAAGTGGAGGTTGAAA+AGG | 0.443488 | 1.2:-25408362 | MS.gene84847:CDS |
| CTTAACTGAGTCAGTTGAAT+CGG | 0.458623 | 1.2:+25408104 | None:intergenic |
| CGATTCAACTGACTCAGTTA+AGG | 0.466446 | 1.2:-25408103 | MS.gene84847:intron |
| TTCGCCAAGCAAACAGAGCT+TGG | 0.466708 | 1.2:+25408264 | None:intergenic |
| AAAACAAATTCTTGTCCTGC+TGG | 0.468712 | 1.2:-25407821 | MS.gene84847:intron |
| GGAACCAAGCTCTGTTTGCT+TGG | 0.476354 | 1.2:-25408268 | MS.gene84847:CDS |
| AGAGAAAGCCACTACGGAAC+TGG | 0.478102 | 1.2:-25408400 | MS.gene84847:CDS |
| CCACTCTTCGGTTTCACCGC+CGG | 0.488504 | 1.2:+25407269 | None:intergenic |
| TTCGCTGGAGGAGGTAGTTA+CGG | 0.493254 | 1.2:-25407333 | MS.gene84847:CDS |
| TGGTGAGGAGAAAGCTTCGC+TGG | 0.494863 | 1.2:-25407348 | MS.gene84847:CDS |
| CGTCACCGTCGTGTCGGAGG+CGG | 0.500832 | 1.2:-25407552 | MS.gene84847:CDS |
| CGAGACGGTGGTTGAAGTTG+CGG | 0.502860 | 1.2:+25408207 | None:intergenic |
| AAGAACAATACCACTAAAGA+TGG | 0.505083 | 1.2:-25408312 | MS.gene84847:CDS |
| GAGTGGTGAAAGAGGAATGA+AGG | 0.505285 | 1.2:-25407252 | MS.gene84847:CDS |
| TGGATTAGCTTCTTTGCTGA+AGG | 0.511501 | 1.2:-25407225 | MS.gene84847:CDS |
| TGGTGGTGGAGTTGCTGAGT+TGG | 0.512259 | 1.2:-25408289 | MS.gene84847:CDS |
| TGCTTTGGCCTAGCTGCCGA+CGG | 0.512808 | 1.2:+25407449 | None:intergenic |
| ACCACTAAAGATGGTGGTGG+TGG | 0.516726 | 1.2:-25408303 | MS.gene84847:CDS |
| GATGCAGGAAATTGCTTCAT+CGG | 0.517243 | 1.2:-25407408 | MS.gene84847:CDS |
| AAGCAGAGTTTGAAGAAGAA+AGG | 0.517627 | 1.2:-25407527 | MS.gene84847:CDS |
| TTCCTGCATCGCCGCCGCAT+CGG | 0.521035 | 1.2:+25407421 | None:intergenic |
| ACCGCCGGTAACTGCCACGC+TGG | 0.521213 | 1.2:+25407284 | None:intergenic |
| GGATCTCTCCGTCGGCAGCT+AGG | 0.526135 | 1.2:-25407457 | MS.gene84847:CDS |
| GAAAATGGTTCAGAGTTTCA+TGG | 0.527012 | 1.2:-25408244 | MS.gene84847:CDS |
| GGAGAAAGCTTCGCTGGAGG+AGG | 0.532608 | 1.2:-25407342 | MS.gene84847:CDS |
| GGTGGTTGAAGTTGCGGTTG+CGG | 0.536574 | 1.2:+25408213 | None:intergenic |
| CTGAATCTATGTCAATCGGT+TGG | 0.551079 | 1.2:+25408421 | None:intergenic |
| TCAATTCATTGATGCGATTG+TGG | 0.553988 | 1.2:-25407708 | MS.gene84847:CDS |
| AAACCGAAGAGTGGTGAAAG+AGG | 0.554832 | 1.2:-25407260 | MS.gene84847:CDS |
| GAAAGAGGAATGAAGGTGAC+TGG | 0.556629 | 1.2:-25407245 | MS.gene84847:CDS |
| GAAGATGATTTCGATTTCAA+CGG | 0.567151 | 1.2:-25408147 | MS.gene84847:CDS |
| ATGTAATCGCGCTTCCTCCA+CGG | 0.582747 | 1.2:+25407491 | None:intergenic |
| TTCATTGATGCGATTGTGGA+AGG | 0.586622 | 1.2:-25407704 | MS.gene84847:CDS |
| GCGGAAAAGCTTCAATCTCT+CGG | 0.590164 | 1.2:-25407878 | MS.gene84847:intron |
| ATGAACCAGTTTCGAAGTGG+AGG | 0.599985 | 1.2:-25408371 | MS.gene84847:CDS |
| GATCGTCACCGTCGTGTCGG+AGG | 0.603839 | 1.2:-25407555 | MS.gene84847:CDS |
| ATTGCGAACCAGTTCCGTAG+TGG | 0.610392 | 1.2:+25408392 | None:intergenic |
| GCAGATCGTCACCGTCGTGT+CGG | 0.612036 | 1.2:-25407558 | MS.gene84847:CDS |
| AGATTCAGAGAAAGCCACTA+CGG | 0.617727 | 1.2:-25408406 | MS.gene84847:CDS |
| AGCGAAGTGGATCTCTCCGT+CGG | 0.622895 | 1.2:-25407465 | MS.gene84847:CDS |
| GAATGCATGTTCCGCCGTGG+AGG | 0.625206 | 1.2:-25407505 | MS.gene84847:CDS |
| AAAGCAAGTCTCCGATGCGG+CGG | 0.625723 | 1.2:-25407432 | MS.gene84847:CDS |
| GAAATCGAAATCATCTTCGT+CGG | 0.627970 | 1.2:+25408152 | None:intergenic |
| GCAATGAACCAGTTTCGAAG+TGG | 0.634899 | 1.2:-25408374 | MS.gene84847:CDS |
| AACAATACCACTAAAGATGG+TGG | 0.638792 | 1.2:-25408309 | MS.gene84847:CDS |
| GCAGGAAATTGCTTCATCGG+AGG | 0.640970 | 1.2:-25407405 | MS.gene84847:CDS |
| TCAATCGGTTGGATCTTCAA+CGG | 0.649259 | 1.2:+25408432 | None:intergenic |
| CTTCGCCGCCTCCGACACGA+CGG | 0.649693 | 1.2:+25407547 | None:intergenic |
| GCGATTACATGTTAGCGAAG+TGG | 0.650775 | 1.2:-25407478 | MS.gene84847:CDS |
| TCCAGCGTGGCAGTTACCGG+CGG | 0.657779 | 1.2:-25407285 | MS.gene84847:CDS |
| CAGTTGCAGCGGTTACGAGA+CGG | 0.659954 | 1.2:+25408192 | None:intergenic |
| CCGGCGGTGAAACCGAAGAG+TGG | 0.661349 | 1.2:-25407269 | MS.gene84847:CDS |
| TAGCGTTGAAACAGTTGCAG+CGG | 0.661984 | 1.2:+25408181 | None:intergenic |
| AATACCACTAAAGATGGTGG+TGG | 0.666485 | 1.2:-25408306 | MS.gene84847:CDS |
| AAGGAATGCATGTTCCGCCG+TGG | 0.669326 | 1.2:-25407508 | MS.gene84847:CDS |
| GGTGGCGACAGCGACAGCGA+CGG | 0.679561 | 1.2:-25407312 | MS.gene84847:CDS |
| TGAGGAGAAAGCTTCGCTGG+AGG | 0.695173 | 1.2:-25407345 | MS.gene84847:CDS |
| GCTGGAGGAGGTAGTTACGG+TGG | 0.712672 | 1.2:-25407330 | MS.gene84847:CDS |
| TTGCAGCGGTTACGAGACGG+TGG | 0.718582 | 1.2:+25408195 | None:intergenic |
| GCCAAAGCAAGTCTCCGATG+CGG | 0.718766 | 1.2:-25407435 | MS.gene84847:CDS |
| CAGCGACGGTGAGTCCAGCG+TGG | 0.734694 | 1.2:-25407298 | MS.gene84847:CDS |
| TAATCGCGCTTCCTCCACGG+CGG | 0.770748 | 1.2:+25407494 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGTTTTTTATTTTGATTT+TGG | - | chr1.2:25407623-25407642 | MS.gene84847:CDS | 10.0% |
| !! | AGAATAGCAAAATTTACAAA+AGG | - | chr1.2:25407728-25407747 | MS.gene84847:intron | 20.0% |
| !!! | ATCGTTTTTTGTATTGTTTT+AGG | - | chr1.2:25407915-25407934 | MS.gene84847:intron | 20.0% |
| ! | ATCCATTTGCAAATCAAAAT+GGG | - | chr1.2:25407806-25407825 | MS.gene84847:intron | 25.0% |
| !!! | TATTGATATCGATTTTCGAT+CGG | - | chr1.2:25407983-25408002 | MS.gene84847:intron | 25.0% |
| AAGAACAATACCACTAAAGA+TGG | - | chr1.2:25407340-25407359 | MS.gene84847:CDS | 30.0% | |
| AAGATTTGATTAAGATCGTG+CGG | - | chr1.2:25407755-25407774 | MS.gene84847:intron | 30.0% | |
| CATCCATTTGCAAATCAAAA+TGG | - | chr1.2:25407805-25407824 | MS.gene84847:intron | 30.0% | |
| GAAGATGATTTCGATTTCAA+CGG | - | chr1.2:25407505-25407524 | MS.gene84847:CDS | 30.0% | |
| TCTTTAGTGGTATTGTTCTT+CGG | + | chr1.2:25407340-25407359 | None:intergenic | 30.0% | |
| TTAGAGAATTAATTACCAGC+AGG | + | chr1.2:25407849-25407868 | None:intergenic | 30.0% | |
| TTCTCTGAATCTATGTCAAT+CGG | + | chr1.2:25407238-25407257 | None:intergenic | 30.0% | |
| ! | TTTTTCCCGACGAAAATATA+CGG | + | chr1.2:25408062-25408081 | None:intergenic | 30.0% |
| AAAACAAATTCTTGTCCTGC+TGG | - | chr1.2:25407831-25407850 | MS.gene84847:CDS | 35.0% | |
| AACAATACCACTAAAGATGG+TGG | - | chr1.2:25407343-25407362 | MS.gene84847:CDS | 35.0% | |
| AAGCAGAGTTTGAAGAAGAA+AGG | - | chr1.2:25408125-25408144 | MS.gene84847:CDS | 35.0% | |
| CTTAACTGAGTCAGTTGAAT+CGG | + | chr1.2:25407551-25407570 | None:intergenic | 35.0% | |
| GAAAATGGTTCAGAGTTTCA+TGG | - | chr1.2:25407408-25407427 | MS.gene84847:CDS | 35.0% | |
| GAAATCGAAATCATCTTCGT+CGG | + | chr1.2:25407503-25407522 | None:intergenic | 35.0% | |
| TCAATTCATTGATGCGATTG+TGG | - | chr1.2:25407944-25407963 | MS.gene84847:intron | 35.0% | |
| TGAAAGTGATTCTGGTGAAT+TGG | - | chr1.2:25408271-25408290 | MS.gene84847:CDS | 35.0% | |
| ! | GTATATTTTCGTCGGGAAAA+AGG | - | chr1.2:25408061-25408080 | MS.gene84847:intron | 35.0% |
| !! | CTTGTGTAAGTGTTGAAGAA+CGG | - | chr1.2:25407671-25407690 | MS.gene84847:CDS | 35.0% |
| !!! | CTCCCATTTTGATTTGCAAA+TGG | + | chr1.2:25407811-25407830 | None:intergenic | 35.0% |
| AGATTCAGAGAAAGCCACTA+CGG | - | chr1.2:25407246-25407265 | MS.gene84847:CDS | 40.0% | |
| ATTCCTCTTTCACCACTCTT+CGG | + | chr1.2:25408398-25408417 | None:intergenic | 40.0% | |
| CGTTCTTCAACACTTACACA+AGG | + | chr1.2:25407673-25407692 | None:intergenic | 40.0% | |
| CTGAATCTATGTCAATCGGT+TGG | + | chr1.2:25407234-25407253 | None:intergenic | 40.0% | |
| GATGCAGGAAATTGCTTCAT+CGG | - | chr1.2:25408244-25408263 | MS.gene84847:CDS | 40.0% | |
| GTGGTATTGTTCTTCGGAAA+AGG | + | chr1.2:25407334-25407353 | None:intergenic | 40.0% | |
| TCAATCGGTTGGATCTTCAA+CGG | + | chr1.2:25407223-25407242 | None:intergenic | 40.0% | |
| TTCATTGATGCGATTGTGGA+AGG | - | chr1.2:25407948-25407967 | MS.gene84847:intron | 40.0% | |
| TTTCGAAGTGGAGGTTGAAA+AGG | - | chr1.2:25407290-25407309 | MS.gene84847:CDS | 40.0% | |
| ! | AATACCACTAAAGATGGTGG+TGG | - | chr1.2:25407346-25407365 | MS.gene84847:CDS | 40.0% |
| ! | CGATTCAACTGACTCAGTTA+AGG | - | chr1.2:25407549-25407568 | MS.gene84847:CDS | 40.0% |
| ! | TCGCTTCCGTATATTTTCGT+CGG | - | chr1.2:25408053-25408072 | MS.gene84847:intron | 40.0% |
| !! | GGTGAATTGGAGCTGATTTT+TGG | - | chr1.2:25408284-25408303 | MS.gene84847:CDS | 40.0% |
| !! | TGGATTAGCTTCTTTGCTGA+AGG | - | chr1.2:25408427-25408446 | MS.gene84847:CDS | 40.0% |
| !!! | ATTGGAGCTGATTTTTGGTG+AGG | - | chr1.2:25408289-25408308 | MS.gene84847:CDS | 40.0% |
| AAACCGAAGAGTGGTGAAAG+AGG | - | chr1.2:25408392-25408411 | MS.gene84847:CDS | 45.0% | |
| ATGAACCAGTTTCGAAGTGG+AGG | - | chr1.2:25407281-25407300 | MS.gene84847:CDS | 45.0% | |
| CTCTGTTTGCTTGGCGAAAA+TGG | - | chr1.2:25407393-25407412 | MS.gene84847:CDS | 45.0% | |
| GAAAGAGGAATGAAGGTGAC+TGG | - | chr1.2:25408407-25408426 | MS.gene84847:CDS | 45.0% | |
| GAGTGGTGAAAGAGGAATGA+AGG | - | chr1.2:25408400-25408419 | MS.gene84847:CDS | 45.0% | |
| GCAATGAACCAGTTTCGAAG+TGG | - | chr1.2:25407278-25407297 | MS.gene84847:CDS | 45.0% | |
| GCGATTACATGTTAGCGAAG+TGG | - | chr1.2:25408174-25408193 | MS.gene84847:CDS | 45.0% | |
| GCGGAAAAGCTTCAATCTCT+CGG | - | chr1.2:25407774-25407793 | MS.gene84847:intron | 45.0% | |
| TCGGAGGTTGAAAGTGATTC+TGG | - | chr1.2:25408263-25408282 | MS.gene84847:CDS | 45.0% | |
| TTCAACCTCCACTTCGAAAC+TGG | + | chr1.2:25407289-25407308 | None:intergenic | 45.0% | |
| ! | CGCTTCCGTATATTTTCGTC+GGG | - | chr1.2:25408054-25408073 | MS.gene84847:intron | 45.0% |
| ! | TAGCGTTGAAACAGTTGCAG+CGG | + | chr1.2:25407474-25407493 | None:intergenic | 45.0% |
| ! | TTTTCGTCGGGAAAAAGGAG+CGG | - | chr1.2:25408066-25408085 | MS.gene84847:intron | 45.0% |
| AGAGAAAGCCACTACGGAAC+TGG | - | chr1.2:25407252-25407271 | MS.gene84847:CDS | 50.0% | |
| ATGTAATCGCGCTTCCTCCA+CGG | + | chr1.2:25408164-25408183 | None:intergenic | 50.0% | |
| ATTGCGAACCAGTTCCGTAG+TGG | + | chr1.2:25407263-25407282 | None:intergenic | 50.0% | |
| GCAGGAAATTGCTTCATCGG+AGG | - | chr1.2:25408247-25408266 | MS.gene84847:CDS | 50.0% | |
| GGAACCAAGCTCTGTTTGCT+TGG | - | chr1.2:25407384-25407403 | MS.gene84847:CDS | 50.0% | |
| TCCACCACCACCATCTTTAG+TGG | + | chr1.2:25407353-25407372 | None:intergenic | 50.0% | |
| TTCGCCAAGCAAACAGAGCT+TGG | + | chr1.2:25407391-25407410 | None:intergenic | 50.0% | |
| TTCGCTGGAGGAGGTAGTTA+CGG | - | chr1.2:25408319-25408338 | MS.gene84847:CDS | 50.0% | |
| !! | ACCACTAAAGATGGTGGTGG+TGG | - | chr1.2:25407349-25407368 | MS.gene84847:CDS | 50.0% |
| AAAGCAAGTCTCCGATGCGG+CGG | - | chr1.2:25408220-25408239 | MS.gene84847:CDS | 55.0% | |
| AAGGAATGCATGTTCCGCCG+TGG | - | chr1.2:25408144-25408163 | MS.gene84847:CDS | 55.0% | |
| AGCGAAGTGGATCTCTCCGT+CGG | - | chr1.2:25408187-25408206 | MS.gene84847:CDS | 55.0% | |
| CAGTTGCAGCGGTTACGAGA+CGG | + | chr1.2:25407463-25407482 | None:intergenic | 55.0% | |
| CGAGACGGTGGTTGAAGTTG+CGG | + | chr1.2:25407448-25407467 | None:intergenic | 55.0% | |
| GCCAAAGCAAGTCTCCGATG+CGG | - | chr1.2:25408217-25408236 | MS.gene84847:CDS | 55.0% | |
| GGTGGTTGAAGTTGCGGTTG+CGG | + | chr1.2:25407442-25407461 | None:intergenic | 55.0% | |
| TGAGGAGAAAGCTTCGCTGG+AGG | - | chr1.2:25408307-25408326 | MS.gene84847:CDS | 55.0% | |
| TGGTGAGGAGAAAGCTTCGC+TGG | - | chr1.2:25408304-25408323 | MS.gene84847:CDS | 55.0% | |
| !! | TGGTGGTGGAGTTGCTGAGT+TGG | - | chr1.2:25407363-25407382 | MS.gene84847:CDS | 55.0% |
| CCACTCTTCGGTTTCACCGC+CGG | + | chr1.2:25408386-25408405 | None:intergenic | 60.0% | |
| GAATGCATGTTCCGCCGTGG+AGG | - | chr1.2:25408147-25408166 | MS.gene84847:CDS | 60.0% | |
| GAGTCCAGCGTGGCAGTTAC+CGG | - | chr1.2:25408364-25408383 | MS.gene84847:CDS | 60.0% | |
| GCAGATCGTCACCGTCGTGT+CGG | - | chr1.2:25408094-25408113 | MS.gene84847:intron | 60.0% | |
| GCTGGAGGAGGTAGTTACGG+TGG | - | chr1.2:25408322-25408341 | MS.gene84847:CDS | 60.0% | |
| GGAGAAAGCTTCGCTGGAGG+AGG | - | chr1.2:25408310-25408329 | MS.gene84847:CDS | 60.0% | |
| TAATCGCGCTTCCTCCACGG+CGG | + | chr1.2:25408161-25408180 | None:intergenic | 60.0% | |
| TGCTTTGGCCTAGCTGCCGA+CGG | + | chr1.2:25408206-25408225 | None:intergenic | 60.0% | |
| TTGCAGCGGTTACGAGACGG+TGG | + | chr1.2:25407460-25407479 | None:intergenic | 60.0% | |
| ! | GCCGCATCGGAGACTTGCTT+TGG | + | chr1.2:25408221-25408240 | None:intergenic | 60.0% |
| CCGGCGGTGAAACCGAAGAG+TGG | - | chr1.2:25408383-25408402 | MS.gene84847:CDS | 65.0% | |
| GATCGTCACCGTCGTGTCGG+AGG | - | chr1.2:25408097-25408116 | MS.gene84847:intron | 65.0% | |
| GGATCTCTCCGTCGGCAGCT+AGG | - | chr1.2:25408195-25408214 | MS.gene84847:CDS | 65.0% | |
| TCCAGCGTGGCAGTTACCGG+CGG | - | chr1.2:25408367-25408386 | MS.gene84847:CDS | 65.0% | |
| TTCCTGCATCGCCGCCGCAT+CGG | + | chr1.2:25408234-25408253 | None:intergenic | 65.0% | |
| ACCGCCGGTAACTGCCACGC+TGG | + | chr1.2:25408371-25408390 | None:intergenic | 70.0% | |
| CAGCGACGGTGAGTCCAGCG+TGG | - | chr1.2:25408354-25408373 | MS.gene84847:CDS | 70.0% | |
| CGTCACCGTCGTGTCGGAGG+CGG | - | chr1.2:25408100-25408119 | MS.gene84847:intron | 70.0% | |
| CTTCGCCGCCTCCGACACGA+CGG | + | chr1.2:25408108-25408127 | None:intergenic | 70.0% | |
| GGTGGCGACAGCGACAGCGA+CGG | - | chr1.2:25408340-25408359 | MS.gene84847:CDS | 70.0% | |
| CTCCGATGCGGCGGCGATGC+AGG | - | chr1.2:25408229-25408248 | MS.gene84847:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 25407214 | 25408460 | 25407214 | ID=MS.gene84847 |
| chr1.2 | mRNA | 25407214 | 25408460 | 25407214 | ID=MS.gene84847.t1;Parent=MS.gene84847 |
| chr1.2 | exon | 25408104 | 25408460 | 25408104 | ID=MS.gene84847.t1.exon1;Parent=MS.gene84847.t1 |
| chr1.2 | CDS | 25408104 | 25408460 | 25408104 | ID=cds.MS.gene84847.t1;Parent=MS.gene84847.t1 |
| chr1.2 | exon | 25407822 | 25407891 | 25407822 | ID=MS.gene84847.t1.exon2;Parent=MS.gene84847.t1 |
| chr1.2 | CDS | 25407822 | 25407891 | 25407822 | ID=cds.MS.gene84847.t1;Parent=MS.gene84847.t1 |
| chr1.2 | exon | 25407214 | 25407737 | 25407214 | ID=MS.gene84847.t1.exon3;Parent=MS.gene84847.t1 |
| chr1.2 | CDS | 25407214 | 25407737 | 25407214 | ID=cds.MS.gene84847.t1;Parent=MS.gene84847.t1 |
| Gene Sequence |
| Protein sequence |
