AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88371


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88371.t1 MTR_2g080990 97.087 103 3 0 1 103 1 103 6.75e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGCAGACTTCTCATTTG+CGG 0.410034 2.3:-17402619 None:intergenic
GGAGCTAAAATCAAGGTTGC+TGG 0.433275 2.3:+17400637 MS.gene88371:CDS
GCAATGGATACATCAGAATC+AGG 0.468029 2.3:+17400616 MS.gene88371:intron
GAGTATACCTTGAGGTGCTT+CGG 0.483494 2.3:-17400454 None:intergenic
GTCGGTTATGTCCATCGAAA+CGG 0.525995 2.3:-17400421 None:intergenic
AACAGGAAGGTGAAGCAGAA+TGG 0.552894 2.3:+17402537 MS.gene88371:intron
TTCAGACTTGGAAACTTTCG+CGG 0.568580 2.3:-17402637 None:intergenic
AGAATCAGGAGCTAAAATCA+AGG 0.594733 2.3:+17400630 MS.gene88371:CDS
GGAAGGTGAAGCAGAATGGA+AGG 0.604858 2.3:+17402541 MS.gene88371:intron
ATCGTTTCCGAAGCACCTCA+AGG 0.606213 2.3:+17400447 MS.gene88371:CDS
GCTAAAATCAAGGTTGCTGG+TGG 0.607057 2.3:+17400640 MS.gene88371:CDS
TGCTTCGGAAACGATGGTGT+CGG 0.636322 2.3:-17400439 None:intergenic
TTGAGGTGCTTCGGAAACGA+TGG 0.645225 2.3:-17400445 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTATAATTTTTTAAAGA+AGG + chr2.3:17401519-17401538 MS.gene88371:intron 10.0%
!! AAAAACAAGAAGAATAAAAA+CGG + chr2.3:17400396-17400415 MS.gene88371:CDS 15.0%
!! AATAAAGAAAACTTACAAAA+TGG - chr2.3:17400671-17400690 None:intergenic 15.0%
!! TTAAACCACAAAAAAAAAAA+AGG - chr2.3:17400570-17400589 None:intergenic 15.0%
!!! ATTTTCCTTTTTTTTTTTTG+TGG + chr2.3:17400562-17400581 MS.gene88371:intron 15.0%
!!! TTATAATTTTTTAAAGAAGG+CGG + chr2.3:17401522-17401541 MS.gene88371:intron 15.0%
!! AAATCAAACTTATTGGATTA+AGG - chr2.3:17401004-17401023 None:intergenic 20.0%
!! AATCAAACTTATTGGATTAA+GGG - chr2.3:17401003-17401022 None:intergenic 20.0%
!! TTAATCCAATAAGTTTGATT+TGG + chr2.3:17401003-17401022 MS.gene88371:intron 20.0%
!! TTGCATAATAATAGTTGTAT+GGG + chr2.3:17402198-17402217 MS.gene88371:intron 20.0%
!! TTTGCATAATAATAGTTGTA+TGG + chr2.3:17402197-17402216 MS.gene88371:intron 20.0%
!!! CATAAAGTTTTAAACAATGA+TGG - chr2.3:17402351-17402370 None:intergenic 20.0%
!!! CATCATTGTTTAAAACTTTA+TGG + chr2.3:17402349-17402368 MS.gene88371:intron 20.0%
!!! TTAATTTGTTTATCCAAACA+AGG - chr2.3:17400865-17400884 None:intergenic 20.0%
! AAATACCAACTTAGAATATG+AGG - chr2.3:17401112-17401131 None:intergenic 25.0%
! AATGATTGTTTGATAGAACA+AGG + chr2.3:17402012-17402031 MS.gene88371:intron 25.0%
! ACAGTTAACATATAACTCAT+AGG - chr2.3:17401925-17401944 None:intergenic 25.0%
! AGTCTATCAATAGGAAAATT+TGG + chr2.3:17402162-17402181 MS.gene88371:intron 25.0%
! TAGAGAATCACAAGTTAAAT+GGG + chr2.3:17400737-17400756 MS.gene88371:intron 25.0%
! TTCTATCAAACAATCATTCA+AGG - chr2.3:17402010-17402029 None:intergenic 25.0%
!! AACCAAAGCATTAAAAAACT+AGG - chr2.3:17400794-17400813 None:intergenic 25.0%
!! ACCAAAGCATTAAAAAACTA+GGG - chr2.3:17400793-17400812 None:intergenic 25.0%
!! TATTATGTTTTGCAAGTAGA+TGG - chr2.3:17402424-17402443 None:intergenic 25.0%
!! TTTAAAACATATATGTGCGT+AGG - chr2.3:17401557-17401576 None:intergenic 25.0%
!!! AAAAAAGTGTCATCAAAATG+AGG - chr2.3:17401846-17401865 None:intergenic 25.0%
!!! AAAAAGTGTCATCAAAATGA+GGG - chr2.3:17401845-17401864 None:intergenic 25.0%
!!! ACTTTTTTTGTTTTCAGACT+TGG - chr2.3:17402652-17402671 None:intergenic 25.0%
!!! TTTTTTAATGCTTTGGTTGA+TGG + chr2.3:17400796-17400815 MS.gene88371:intron 25.0%
AAAACTGATGAACAATTCAC+AGG - chr2.3:17402251-17402270 None:intergenic 30.0%
AATATTCTATACTTTCGCAG+AGG + chr2.3:17402276-17402295 MS.gene88371:intron 30.0%
ACTTAGAATATGAGGAGATA+TGG - chr2.3:17401104-17401123 None:intergenic 30.0%
AGAGAATCACAAGTTAAATG+GGG + chr2.3:17400738-17400757 MS.gene88371:intron 30.0%
AGCCAAATAGAGAGTTAAAT+AGG + chr2.3:17402069-17402088 MS.gene88371:intron 30.0%
ATAACTGACAATAGTTAGCT+AGG + chr2.3:17402122-17402141 MS.gene88371:intron 30.0%
ATATCAATCGAGTCTATGAT+AGG + chr2.3:17402042-17402061 MS.gene88371:intron 30.0%
ATCACTTAACTGTGTGATAT+AGG + chr2.3:17401189-17401208 MS.gene88371:intron 30.0%
ATCATAGACTCGATTGATAT+CGG - chr2.3:17402042-17402061 None:intergenic 30.0%
ATTCAAGGCAAAAATTCTGA+TGG - chr2.3:17401995-17402014 None:intergenic 30.0%
CATGTTATAATTGAGGACAT+TGG - chr2.3:17401957-17401976 None:intergenic 30.0%
CCAAATAGAGAGTTAAATAG+GGG + chr2.3:17402071-17402090 MS.gene88371:intron 30.0%
CTTACAAAATTGATCAGCAT+TGG - chr2.3:17401736-17401755 None:intergenic 30.0%
CTTATTCATAACCACTCATA+TGG - chr2.3:17400901-17400920 None:intergenic 30.0%
GAATACGAACAATTCACTAA+TGG + chr2.3:17401701-17401720 MS.gene88371:intron 30.0%
GACGAAGGCTAAAAATATAA+GGG - chr2.3:17400695-17400714 None:intergenic 30.0%
GCCAAATAGAGAGTTAAATA+GGG + chr2.3:17402070-17402089 MS.gene88371:intron 30.0%
GTAGAGAATCACAAGTTAAA+TGG + chr2.3:17400736-17400755 MS.gene88371:intron 30.0%
TATCTCCTCATATTCTAAGT+TGG + chr2.3:17401104-17401123 MS.gene88371:intron 30.0%
TGTTCGTATTCTAATTACAC+AGG - chr2.3:17401692-17401711 None:intergenic 30.0%
TTGCACCAAATCAAACTTAT+TGG - chr2.3:17401011-17401030 None:intergenic 30.0%
! ATAGTAGTTGCCTTGTATTT+TGG + chr2.3:17401460-17401479 MS.gene88371:intron 30.0%
! ATTGGATGTTGTTGTTGAAT+TGG + chr2.3:17401061-17401080 MS.gene88371:intron 30.0%
! TAGTAGTTGCCTTGTATTTT+GGG + chr2.3:17401461-17401480 MS.gene88371:intron 30.0%
! TTGGATGTTGTTGTTGAATT+GGG + chr2.3:17401062-17401081 MS.gene88371:intron 30.0%
!! AACACTTTTTGATCTCTTCA+TGG - chr2.3:17402568-17402587 None:intergenic 30.0%
!! AATCACATTTACTTTTGCAC+TGG + chr2.3:17401330-17401349 MS.gene88371:intron 30.0%
!! ACACTTTTTGATCTCTTCAT+GGG - chr2.3:17402567-17402586 None:intergenic 30.0%
!! CTTTTGTTTATTTGCAGCAA+TGG + chr2.3:17400600-17400619 MS.gene88371:intron 30.0%
!! TATTCTTTAAAGCATGCACT+TGG + chr2.3:17401410-17401429 MS.gene88371:intron 30.0%
!! TGTATGGGATATTTGTTATC+AGG + chr2.3:17402213-17402232 MS.gene88371:intron 30.0%
!!! ACCCTAGTTTTTTAATGCTT+TGG + chr2.3:17400789-17400808 MS.gene88371:intron 30.0%
!!! GCAATGTAGTTTTTTTTGCA+TGG + chr2.3:17401288-17401307 MS.gene88371:intron 30.0%
AATCTCACATGTGAATATGC+TGG + chr2.3:17400935-17400954 MS.gene88371:intron 35.0%
ACTGTGTGATATAGGAAATC+TGG + chr2.3:17401197-17401216 MS.gene88371:intron 35.0%
AGAATCAGGAGCTAAAATCA+AGG + chr2.3:17400630-17400649 MS.gene88371:CDS 35.0%
AGTCATCATTCTTTGTCGAA+CGG + chr2.3:17401161-17401180 MS.gene88371:intron 35.0%
AGTTGAACGCAATTATAGGA+GGG - chr2.3:17401231-17401250 None:intergenic 35.0%
ATCTGTTGAGTTCTTGAAGA+AGG + chr2.3:17401386-17401405 MS.gene88371:intron 35.0%
CAAAGCAAACCCAAAATACA+AGG - chr2.3:17401473-17401492 None:intergenic 35.0%
CCAATAATCAGTTGAGTAAC+TGG - chr2.3:17401046-17401065 None:intergenic 35.0%
CCAGTTACTCAACTGATTAT+TGG + chr2.3:17401043-17401062 MS.gene88371:intron 35.0%
CCATTGCTACAATGAAGAAA+TGG - chr2.3:17401269-17401288 None:intergenic 35.0%
CCATTTCTTCATTGTAGCAA+TGG + chr2.3:17401266-17401285 MS.gene88371:intron 35.0%
CCCCTATTTAACTCTCTATT+TGG - chr2.3:17402074-17402093 None:intergenic 35.0%
CGACGAAGGCTAAAAATATA+AGG - chr2.3:17400696-17400715 None:intergenic 35.0%
CTGTGTGATATAGGAAATCT+GGG + chr2.3:17401198-17401217 MS.gene88371:intron 35.0%
GCAACGATTTGAGAAAATGT+TGG - chr2.3:17400834-17400853 None:intergenic 35.0%
GTACAGTTGAACGCAATTAT+AGG - chr2.3:17401235-17401254 None:intergenic 35.0%
TAATCAGTTGAGTAACTGGA+CGG - chr2.3:17401042-17401061 None:intergenic 35.0%
TCATAGACTCGATTGATATC+GGG - chr2.3:17402041-17402060 None:intergenic 35.0%
TCGCGAAGTATATTACAGAA+GGG - chr2.3:17402308-17402327 None:intergenic 35.0%
TCTGTTGAGTTCTTGAAGAA+GGG + chr2.3:17401387-17401406 MS.gene88371:intron 35.0%
TTACACAGGCAATTTAGTGA+TGG - chr2.3:17401678-17401697 None:intergenic 35.0%
TTCTCAAATCGTTGCAATTC+AGG + chr2.3:17400839-17400858 MS.gene88371:intron 35.0%
TTGCTCTTCTGTTTAGTCAT+GGG + chr2.3:17401895-17401914 MS.gene88371:intron 35.0%
TTTGCAAGTAGATGGAAATC+AGG - chr2.3:17402416-17402435 None:intergenic 35.0%
TTTGCTCTTCTGTTTAGTCA+TGG + chr2.3:17401894-17401913 MS.gene88371:intron 35.0%
! ATAATGTTTTCTGCTCTGCT+GGG - chr2.3:17401651-17401670 None:intergenic 35.0%
!! TTACTTTTGCACTGGATGTT+AGG + chr2.3:17401338-17401357 MS.gene88371:intron 35.0%
AAGTTAAATGGGGTTTAGCG+CGG + chr2.3:17400748-17400767 MS.gene88371:intron 40.0%
ACTGATGAACAATTCACAGG+AGG - chr2.3:17402248-17402267 None:intergenic 40.0%
AGTTAGCTAGGAAGAGTAGA+TGG + chr2.3:17402134-17402153 MS.gene88371:intron 40.0%
AGTTCAGTTCGCATAGTTGA+TGG + chr2.3:17401791-17401810 MS.gene88371:intron 40.0%
ATGGATGGGAGTCTATCAAT+AGG + chr2.3:17402153-17402172 MS.gene88371:intron 40.0%
CAGTTGAACGCAATTATAGG+AGG - chr2.3:17401232-17401251 None:intergenic 40.0%
CTCGCGAAGTATATTACAGA+AGG - chr2.3:17402309-17402328 None:intergenic 40.0%
GCAATGGATACATCAGAATC+AGG + chr2.3:17400616-17400635 MS.gene88371:intron 40.0%
GTGTTTACTCCACGACATAT+TGG - chr2.3:17402380-17402399 None:intergenic 40.0%
TCACATGTGAATATGCTGGT+AGG + chr2.3:17400939-17400958 MS.gene88371:intron 40.0%
TCCAGCGCATGTTATAATTG+AGG - chr2.3:17401964-17401983 None:intergenic 40.0%
TCCTCAATTATAACATGCGC+TGG + chr2.3:17401960-17401979 MS.gene88371:intron 40.0%
TGAAAAGCGAGTATACCTTG+AGG - chr2.3:17400465-17400484 None:intergenic 40.0%
TTCAGACTTGGAAACTTTCG+CGG - chr2.3:17402640-17402659 None:intergenic 40.0%
! GATAATGTTTTCTGCTCTGC+TGG - chr2.3:17401652-17401671 None:intergenic 40.0%
!! TGGATGTTAGGTTCTAGACT+TGG + chr2.3:17401350-17401369 MS.gene88371:intron 40.0%
AACAATTCACAGGAGGTTCG+AGG - chr2.3:17402241-17402260 None:intergenic 45.0%
AACAGGAAGGTGAAGCAGAA+TGG + chr2.3:17402537-17402556 MS.gene88371:intron 45.0%
ACGCAATTATAGGAGGGAAG+AGG - chr2.3:17401225-17401244 None:intergenic 45.0%
AGCTAGGAAGAGTAGATGGA+TGG + chr2.3:17402138-17402157 MS.gene88371:intron 45.0%
CGCAATTATAGGAGGGAAGA+GGG - chr2.3:17401224-17401243 None:intergenic 45.0%
GCTAAAATCAAGGTTGCTGG+TGG + chr2.3:17400640-17400659 MS.gene88371:CDS 45.0%
GCTAGGAAGAGTAGATGGAT+GGG + chr2.3:17402139-17402158 MS.gene88371:intron 45.0%
GGAGCTAAAATCAAGGTTGC+TGG + chr2.3:17400637-17400656 MS.gene88371:CDS 45.0%
GTAGATGGAAATCAGGAAGC+AGG - chr2.3:17402409-17402428 None:intergenic 45.0%
GTCGGTTATGTCCATCGAAA+CGG - chr2.3:17400424-17400443 None:intergenic 45.0%
TGCAGCTTCTACCATATGAG+TGG + chr2.3:17400887-17400906 MS.gene88371:intron 45.0%
! ATTATCAAGCATCGCGACGA+AGG - chr2.3:17400710-17400729 None:intergenic 45.0%
! GAGTATACCTTGAGGTGCTT+CGG - chr2.3:17400457-17400476 None:intergenic 45.0%
!! GTTGCAATTCAGGCCTTGTT+TGG + chr2.3:17400849-17400868 MS.gene88371:intron 45.0%
!! TAAAAACGGTGCCGTTTCGA+TGG + chr2.3:17400410-17400429 MS.gene88371:CDS 45.0%
!!! GCATGCTTTTGCTCTGAAAC+AGG + chr2.3:17402520-17402539 MS.gene88371:intron 45.0%
!!! GCTTTTGCTCTGAAACAGGA+AGG + chr2.3:17402524-17402543 MS.gene88371:intron 45.0%
ATGGTGCGTCCAATATGTCG+TGG + chr2.3:17402368-17402387 MS.gene88371:intron 50.0%
GGAAGGTGAAGCAGAATGGA+AGG + chr2.3:17402541-17402560 MS.gene88371:intron 50.0%
TGCTTCGGAAACGATGGTGT+CGG - chr2.3:17400442-17400461 None:intergenic 50.0%
! ATCGTTTCCGAAGCACCTCA+AGG + chr2.3:17400447-17400466 MS.gene88371:CDS 50.0%
! TTGAGGTGCTTCGGAAACGA+TGG - chr2.3:17400448-17400467 None:intergenic 50.0%
!! ACTTGGTGCACTGCAAGATG+TGG + chr2.3:17401427-17401446 MS.gene88371:intron 50.0%
CGCGGCAGACTTCTCATTTG+CGG - chr2.3:17402622-17402641 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 17400378 17402704 17400378 ID=MS.gene88371
chr2.3 mRNA 17400378 17402704 17400378 ID=MS.gene88371.t1;Parent=MS.gene88371
chr2.3 exon 17400378 17400468 17400378 ID=MS.gene88371.t1.exon1;Parent=MS.gene88371.t1
chr2.3 CDS 17400378 17400468 17400378 ID=cds.MS.gene88371.t1;Parent=MS.gene88371.t1
chr2.3 exon 17400617 17400674 17400617 ID=MS.gene88371.t1.exon2;Parent=MS.gene88371.t1
chr2.3 CDS 17400617 17400674 17400617 ID=cds.MS.gene88371.t1;Parent=MS.gene88371.t1
chr2.3 exon 17402542 17402704 17402542 ID=MS.gene88371.t1.exon3;Parent=MS.gene88371.t1
chr2.3 CDS 17402542 17402704 17402542 ID=cds.MS.gene88371.t1;Parent=MS.gene88371.t1
Gene Sequence

>MS.gene88371

ATGGCGAAAAACAGGAACAAAAACAAGAAGAATAAAAACGGTGCCGTTTCGATGGACATAACCGACACCATCGTTTCCGAAGCACCTCAAGGTATACTCGCTTTTCAATTAACAACTAAAATTTCAATTTTTTTACTTTTATTTCTCTGCTTCTAATCTCATAGTGAACTCTTGTTTTGTTTTAATTTTCCTTTTTTTTTTTTGTGGTTTAAGATTTTGAGACTTTTGTTTATTTGCAGCAATGGATACATCAGAATCAGGAGCTAAAATCAAGGTTGCTGGTGGTGCTACCATTTTGTAAGTTTTCTTTATTGCCCTTATATTTTTAGCCTTCGTCGCGATGCTTGATAATGATATTGTAGAGAATCACAAGTTAAATGGGGTTTAGCGCGGTTGTGAGCTTGTTTTAAAACCCTAGTTTTTTAATGCTTTGGTTGATGGAACAGAATGATACCAACATTTTCTCAAATCGTTGCAATTCAGGCCTTGTTTGGATAAACAAATTAATTTGCAGCTTCTACCATATGAGTGGTTATGAATAAGCTATTTCTATTAACAATCTCACATGTGAATATGCTGGTAGGTAAGCTCAAATACGTTAATAATAGTTAATTCAGACATACCCTTAATCCAATAAGTTTGATTTGGTGCAACATAATTACCGTCCAGTTACTCAACTGATTATTGGATGTTGTTGTTGAATTGGGCATTGTCATCATGATGCCATATCTCCTCATATTCTAAGTTGGTATTTTAGAGTCGATGTAGTGAAGTTAGATCATGAGTCATCATTCTTTGTCGAACGGTTGAGATCACTTAACTGTGTGATATAGGAAATCTGGGCCCTCTTCCCTCCTATAATTGCGTTCAACTGTACACACGTTACTGCCATTTCTTCATTGTAGCAATGGCAATGTAGTTTTTTTTGCATGGACAAAACAACTGTATCTTCAATCACATTTACTTTTGCACTGGATGTTAGGTTCTAGACTTGGTTTTGATTACTTGATCTGTTGAGTTCTTGAAGAAGGGTATTCTTTAAAGCATGCACTTGGTGCACTGCAAGATGTGGTTTGTTAGTAATAGTAGTTGCCTTGTATTTTGGGTTTGCTTTGAACTTTATAAATACAAATTCGATGTAGTATTATAATTTTTTAAAGAAGGCGGTGAATATATCCTACGCACATATATGTTTTAAACATATTTTTTGTATTAAACAAACTCTTATGAGTCAAGTCTATAGACATTTAATGAGTCAAGTTTTATGAGACCCAGCAGAGCAGAAAACATTATCTTTCCATCACTAAATTGCCTGTGTAATTAGAATACGAACAATTCACTAATGGACAATGTTACCAATGCTGATCAATTTTGTAAGTGCAATATTTTGTTTAACAAAAATTTATATACTTCAGTTCAGTTCGCATAGTTGATGGATTGTGTACAATGCTTTTTCTGCATTGTCCCTCATTTTGATGACACTTTTTTTTTTTTTTTTTGAATACACTTGCTTTGTTTTGCTCTTCTGTTTAGTCATGGGCATGCCTATGAGTTATATGTTAACTGTTAGTTGTTGCCAATGTCCTCAATTATAACATGCGCTGGATCTGAAGTCCATCAGAATTTTTGCCTTGAATGATTGTTTGATAGAACAAGGCAACCCGATATCAATCGAGTCTATGATAGGAGAAAGCCAAATAGAGAGTTAAATAGGGGAAGTTATTTGTCATAACTGTCGATATTTATAACTGACAATAGTTAGCTAGGAAGAGTAGATGGATGGGAGTCTATCAATAGGAAAATTTGGTAGAGAGAAAATTTTGCATAATAATAGTTGTATGGGATATTTGTTATCAGGTTCCTCGAACCTCCTGTGAATTGTTCATCAGTTTTCAATAAATATTCTATACTTTCGCAGAGGAGATTTCCCTTCTGTAATATACTTCGCGAGTTCTAATGTGATTCAAGTTCCATCATTGTTTAAAACTTTATGGTGCGTCCAATATGTCGTGGAGTAAACACATATTACCTGCTTCCTGATTTCCATCTACTTGCAAAACATAATAATGCTCAAGTTCTTCTCATCTCGCACATTTAAAAGTTACTGTATGATAGTATTGCATTCTTACTGCATTTTCAAATGCATGCTTTTGCTCTGAAACAGGAAGGTGAAGCAGAATGGAAGGCCCATGAAGAGATCAAAAAGTGTTCGAAAGATGAAGAAAATAGCAAAAGCTATATCCGCAAATGAGAAGTCTGCCGCGAAAGTTTCCAAGTCTGAAAACAAAAAAAGTAGAGTTCAATCTGCGAAAACACTCTACGAATAA

Protein sequence

>MS.gene88371.t1

MAKNRNKNKKNKNGAVSMDITDTIVSEAPQAMDTSESGAKIKVAGGATILKVKQNGRPMKRSKSVRKMKKIAKAISANEKSAAKVSKSENKKSRVQSAKTLYE