AlfalfaGEDB | MS.gene88959

M. sativa cultivar XinJiangDaYe / MS.gene88959

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016787 GO:0016788
PFAM: PF00657.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88959.t1	AES59779.1	98.4	311	5	0	1	311	59	369	8.60E-180	639.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88959.t1	Q94CH8	54.0	313	142	2	1	311	61	373	1.0e-96	354.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88959.t1	G7I7S7	98.4	311	5	0	1	311	59	369	6.2e-180	639.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene88959	0.800871	1.18E-48	-1.69E-46
MS.gene049346	MS.gene88959	0.809674	1.68E-50	-1.69E-46
MS.gene049758	MS.gene88959	0.820443	6.84E-53	-1.69E-46
MS.gene050099	MS.gene88959	0.809391	1.94E-50	-1.69E-46
MS.gene050128	MS.gene88959	0.828751	7.55E-55	-1.69E-46
MS.gene050301	MS.gene88959	0.861685	8.62E-64	-1.69E-46
MS.gene050397	MS.gene88959	0.821381	4.16E-53	-1.69E-46
MS.gene050404	MS.gene88959	0.810173	1.31E-50	-1.69E-46
MS.gene050406	MS.gene88959	0.818517	1.88E-52	-1.69E-46
MS.gene050529	MS.gene88959	0.802901	4.50E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88959.t1	MTR_1g025700	98.077	312	6	0	1	312	59	370	0.0	635
MS.gene88959.t1	MTR_1g025720	77.636	313	70	0	1	313	46	358	0.0	528
MS.gene88959.t1	MTR_1g025680	73.885	314	81	1	1	313	43	356	9.30e-179	502
MS.gene88959.t1	MTR_3g114090	66.879	314	103	1	1	313	45	358	5.26e-158	446
MS.gene88959.t1	MTR_1g025780	63.578	313	113	1	1	313	50	361	3.46e-154	436
MS.gene88959.t1	MTR_1g025760	63.259	313	114	1	2	313	90	402	1.66e-152	433
MS.gene88959.t1	MTR_3g114120	62.500	312	117	0	1	312	56	367	2.17e-150	427
MS.gene88959.t1	MTR_5g022630	51.899	316	149	3	1	313	52	367	3.91e-117	342
MS.gene88959.t1	MTR_8g075230	49.682	314	157	1	1	313	45	358	1.66e-116	340
MS.gene88959.t1	MTR_5g022640	49.841	315	156	2	1	313	52	366	6.71e-115	336

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88959.t1	AT1G75880	53.503	314	145	1	1	313	61	374	2.71e-122	355
MS.gene88959.t1	AT1G75880	53.651	315	144	2	1	313	61	375	1.35e-121	354
MS.gene88959.t1	AT1G75900	50.637	314	153	2	1	313	52	364	2.00e-120	350
MS.gene88959.t1	AT1G75890	51.447	311	149	2	1	309	56	366	1.16e-118	346
MS.gene88959.t1	AT1G75890	51.447	311	149	2	1	309	85	395	1.46e-118	347
MS.gene88959.t1	AT5G42170	51.592	314	148	2	1	313	59	369	1.18e-116	341
MS.gene88959.t1	AT1G75890	49.383	324	149	3	1	309	56	379	2.85e-115	338
MS.gene88959.t1	AT3G14820	49.201	313	154	3	1	312	41	349	4.33e-110	323
MS.gene88959.t1	AT1G59406	50.318	314	150	4	1	312	39	348	5.26e-110	323
MS.gene88959.t1	AT1G59030	50.318	314	150	4	1	312	39	348	5.26e-110	323

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Find 75 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACATAATCCAAATCGT+TGG	0.574159	1.4:-72645	None:intergenic
AAATCTCCAGCTTAGTGAAT+TGG	0.402444	1.4:+70450	MS.gene88959:CDS
AAATGTATCAACTAGGTGCA+AGG	0.593045	1.4:+71379	MS.gene88959:CDS
AACACATAATCCAAATCGTT+GGG	0.476159	1.4:-72644	None:intergenic
AACGGTACACACCCCATTGG+TGG	0.566232	1.4:-71423	None:intergenic
AACTTTGCATCCGGTGGTGC+AGG	0.406962	1.4:+70484	MS.gene88959:CDS
AAGTAGCAGGATTGTTTACA+TGG	0.453720	1.4:+71563	MS.gene88959:CDS
AATCTTTGAATAAGTCTAGC+TGG	0.512632	1.4:-70903	None:intergenic
AATTCACTAAGCTGGAGATT+TGG	0.341715	1.4:-70448	None:intergenic
ACAACATGACAACACCATCT+CGG	0.621905	1.4:+69756	MS.gene88959:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACACATAATCCAAATCGT+TGG	-	chr1.4:72648-72667	None:intergenic	30.0%
AACACATAATCCAAATCGTT+GGG	-	chr1.4:72647-72666	None:intergenic	30.0%
AATCTAAACCTCTCAAAGTA+AGG	-	chr1.4:71928-71947	None:intergenic	30.0%
AATCTTTGAATAAGTCTAGC+TGG	-	chr1.4:70906-70925	None:intergenic	30.0%
ACTATAACACTATGTTTGGA+AGG	-	chr1.4:70118-70137	None:intergenic	30.0%
AGGCATTAAAGAATATCTAC+CGG	+	chr1.4:70414-70433	MS.gene88959:CDS	30.0%
ATTCGAGGACTAAACTAAAA+AGG	-	chr1.4:70586-70605	None:intergenic	30.0%
CATTTCATCAAGTTTGTCAA+TGG	+	chr1.4:70639-70658	MS.gene88959:intron	30.0%
TAAAATCTTTCCCATATGGA+GGG	-	chr1.4:69790-69809	None:intergenic	30.0%
TACCACAAGAAAAAGACTAT+TGG	-	chr1.4:70983-71002	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	69737	72751	69737	ID=MS.gene88959
chr1.4	mRNA	69737	72751	69737	ID=MS.gene88959.t1;Parent=MS.gene88959
chr1.4	exon	69737	69866	69737	ID=MS.gene88959.t1.exon1;Parent=MS.gene88959.t1
chr1.4	CDS	69737	69866	69737	ID=cds.MS.gene88959.t1;Parent=MS.gene88959.t1
chr1.4	exon	70404	70534	70404	ID=MS.gene88959.t1.exon2;Parent=MS.gene88959.t1
chr1.4	CDS	70404	70534	70404	ID=cds.MS.gene88959.t1;Parent=MS.gene88959.t1
chr1.4	exon	70883	71116	70883	ID=MS.gene88959.t1.exon3;Parent=MS.gene88959.t1
chr1.4	CDS	70883	71116	70883	ID=cds.MS.gene88959.t1;Parent=MS.gene88959.t1
chr1.4	exon	71378	71633	71378	ID=MS.gene88959.t1.exon4;Parent=MS.gene88959.t1
chr1.4	CDS	71378	71633	71378	ID=cds.MS.gene88959.t1;Parent=MS.gene88959.t1
chr1.4	exon	72561	72751	72561	ID=MS.gene88959.t1.exon5;Parent=MS.gene88959.t1
chr1.4	CDS	72561	72751	72561	ID=cds.MS.gene88959.t1;Parent=MS.gene88959.t1

Gene Sequence

>MS.gene88959

ATGGATACTGGAAATAACAACAACATGACAACACCATCTCGGTGCAATTACCCTCCATATGGGAAAGATTTTAAAGGAGGAATTCCAACAGGAAGATTTAGCAACGGAAAGGTTCCGTCGGATTTTGTAGGTATATATTATTCAATCTTATTACACATATCAATATATGAAATAAAAAAGGGATTATAAATAGTTACTTATAGTAGGATAAAAGATACTTCTTCCCATTTTCTTTGCATCTGAGTGAAGAGACCTAAAAGTGGTAGGCCCCACCAATTTGGTCTCTCTCGTCTCTGTTTCTCTCCTATACCAAACACGGTGGTCATGGTCTTTCTCTCCACTTTCTCTCGTAGGCATCTCTCTCTTTGCAAAATCTCTCCTTCCAAACATAGTGTTATAGTTACATTTTGCTTGGAATTAGTCTGTTAAGCTTATAATAAGTTATATAAATATGCAAAATATGAGTTAGACTCTTTTGCAACAAAATATACATAACATCTCCCTAACAAAAAATATAAGTAAAAGAAGTTGTATTTGGTCCATTCATTGATTAAACCTATACTAATTTTTGTTGCAGTGCTATAACTTATAAATTAAATTGTTGAATCCAAGATGTGTGTATGTTAATATAGTTGTTTTTTCTTTTCAATTTAAATTGTATTATCAGTTGAAGAATTAGGCATTAAAGAATATCTACCGGCATATTTGGATCCAAATCTCCAGCTTAGTGAATTGGCCACTGGCGTTAACTTTGCATCCGGTGGTGCAGGATATGATCCTTTAACAGCAAAATTAGAGGTATACATATTTTTAGAATAAATTATCAATTTGGTCTTGAATATTACCCCTTTTTAGTTTAGTCCTCGAATTATAAAAATATAATAAATAGTCTCAACTATTTTCATTTCATCAAGTTTGTCAATGGACTATTATTCTCTCTCTTTTTTGAACAAATTAAGCTATTAACCTGATTGATTAATAATAATCATAAATTTAAGACTATTTACTATACTTTAATCTTAATTTAGAGACTAAATTAAGTAAAGGGCAACAACTCATGGACAGATTGAAGTTTTATTTCATATTTTCATGAACAAAAAATTAACAAGTTTCATATTTGGTATTTCATGTATATTTATATAACAGGTGGCTATATCTATGTCTGGCCAGCTAGACTTATTCAAAGATTACATTGTGAGGCTTAAAGGACTAGTTGGTGAAGATAGAGCAAATTTCATCTTAGCCAATAGTCTTTTTCTTGTGGTACTAGGAAGCAATGACATTTCTAACACGTACTACTTGTCTCATTTAAGACAAGCACAATATGACTTTCCTACATACTCTGACCTTTTGGTCAACTCAGCTTTAAATTTCTACCAGGTACATCTATATAATTTTATTATTTTATATACTGAAAATTTATTGAGTGTCTTAAAAACATTGTGTTTTCAATAAATAGAAAATGAAAATATTTTGAAGTTTTTCAATAAATAATTTATTTATTTAAAATATTTAATCAATGTACTTACAACACCTGTAGCAGCACTTTTTTATATATGTGATGCTGGTAATGGTTATTTAAATGTTAACTTTGAAAATGTAATTCATTTATTTAATTTTTCTTGTGTTTACAATATAAAGGAAATGTATCAACTAGGTGCAAGGAGGATAGGAGTATTTAATGCACCACCAATGGGGTGTGTACCGTTTCAGAGAACAATGGCAGGAGGGATAATAAGAACATGTGTGCAAGAGTACAATGATGCAGCTGTGTTTTTTAACAACAAGTTGTCAATTGGGATTGATACCTTTAAGCGGAATTTTCCAAGTAGCAGGATTGTTTACATGGATGTTTACAACCCTCTACTCGATATTATTGTAAACAACCAAAAATATGGTAGTAATTTCAATTTTCACTTTGTTTTAAGTTTTATACTAAGTCTAACTCAATCTTATAAAAACGGCTTGTATGGTGAAGAGTGACACTTTAAAAAAAATATTAATTAGATCCTAGAAAACATGGACACATAACACAAAACAGACACTGACACATCGACATCTATTATCATTTAAGAAAATGACATAATTATAAGTGTCAATGTTATGTCGAGTTGAACACCAAACATGTGTCCGACACCGGAACACACATAATTTAAAAAGTGTTTGTGCTTCATAGATTATAAATTATCCTTACTTTGAGAGGTTTAGATTTTTTCCAATAATATATCATCTTAATAGATTATTCACAATCAATCAATTTAAGAAATCAATCATAAAATAATTATGTTGCAAAATGAATACTTCTATTTTAGGAGTGTTAGTAATTCACTATGTTTTTTATTAGATAAAACTCATGTCGCTATATCTCGTTATTTTGAGAGTGAATACGATAAAAATAGTAGAATCCACATAAAATTCTCTTAATTTAATTAGAAAGAAGTGTTAGAGAGGAGAAACTCTTCTATTCACAAATGATAAATACTTAGACACACTCACATCAAATGCAAAAATTAAAAGAGAATAAAACTAAATGATGTTGATTGATGTGATTTAATTATTTTTGTTTTAAATTTTTCCATTTGATGTGAGTGTGTCTAAATATTTATCATTTGTAGTTGTGTCCATAGAAGAGTTTCTCGTTAGAGAGTGTGTAGCTGTCAATAAAAAAAGATAGAAAATGTGTAGCTATCACTTTTCCTAATTTCATATTGATTTCATTTATCTTTTAAAAATATCTTAGTGTTTTTGTGGAAAGAAAAATTTAATGCTTTTATCAATTGATTGAAGATTTAACCTTTGAAAAAAAAATCTTTTCAACTTGTAGGGTATGAAGTTGGAGACAGAGGATGTTGTGGCACAGGCACACTAGAAGTTACTTATTTATGCAACCATTTGCAACCCACTTGTCCCAACGATTTGGATTATGTGTTTTGGGACAGTTTTCACCCTACTGAATCCGTTTACAGAAAACTTGTTGCCCCTATTCTACAGAAATACATGCAGCAGTTACTGTGA

Protein sequence

>MS.gene88959.t1

MDTGNNNNMTTPSRCNYPPYGKDFKGGIPTGRFSNGKVPSDFVVEELGIKEYLPAYLDPNLQLSELATGVNFASGGAGYDPLTAKLEVAISMSGQLDLFKDYIVRLKGLVGEDRANFILANSLFLVVLGSNDISNTYYLSHLRQAQYDFPTYSDLLVNSALNFYQEMYQLGARRIGVFNAPPMGCVPFQRTMAGGIIRTCVQEYNDAAVFFNNKLSIGIDTFKRNFPSSRIVYMDVYNPLLDIIVNNQKYGYEVGDRGCCGTGTLEVTYLCNHLQPTCPNDLDYVFWDSFHPTESVYRKLVAPILQKYMQQLL