AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89511


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89511.t1 MTR_5g062650 95.531 179 8 0 1 179 607 785 1.55e-109 330
MS.gene89511.t1 MTR_5g062430 95.531 179 8 0 1 179 604 782 1.48e-108 327
MS.gene89511.t1 MTR_5g062370 94.413 179 10 0 1 179 604 782 1.77e-107 325
MS.gene89511.t1 MTR_5g062320 93.296 179 12 0 1 179 602 780 6.17e-106 320
MS.gene89511.t1 MTR_3g074060 63.687 179 65 0 1 179 598 776 7.39e-81 255
MS.gene89511.t1 MTR_5g030860 38.172 186 106 2 1 179 599 782 1.25e-35 131
MS.gene89511.t1 MTR_2g034720 36.932 176 100 3 1 175 587 752 7.80e-31 117
MS.gene89511.t1 MTR_2g008240 34.254 181 108 4 1 179 604 775 1.36e-30 117
MS.gene89511.t1 MTR_1g106860 34.359 195 103 4 1 180 591 775 2.06e-29 114
MS.gene89511.t1 MTR_1g087240 34.595 185 100 4 1 177 584 755 9.31e-28 108
MS.gene89511.t1 MTR_4g094285 34.595 185 102 4 1 179 603 774 9.61e-28 108
MS.gene89511.t1 MTR_7g109390 35.676 185 99 6 1 177 589 761 7.26e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89511.t1 AT1G78060 51.381 181 81 4 1 179 592 767 1.83e-56 189
MS.gene89511.t1 AT5G10560 38.122 181 108 1 1 177 610 790 2.48e-36 133
MS.gene89511.t1 AT3G19620 36.782 174 97 4 1 169 593 758 4.98e-28 110
MS.gene89511.t1 AT5G09700 37.912 182 105 3 1 179 350 526 1.76e-27 107
MS.gene89511.t1 AT5G09730 36.957 184 104 4 1 179 597 773 2.75e-27 107
MS.gene89511.t1 AT1G02640 34.463 177 107 3 1 177 591 758 3.12e-26 104
MS.gene89511.t1 AT5G64570 36.364 187 102 4 1 179 607 784 1.23e-25 103
MS.gene89511.t1 AT5G64570 36.364 187 102 4 1 179 449 626 1.26e-25 102
MS.gene89511.t1 AT5G49360 35.326 184 100 7 1 175 595 768 2.07e-24 99.8

Find 51 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCATTTCTACCTTTCTT+TGG 0.223144 5.4:+48817129 None:intergenic
AGCAAATGAGAGTGGTGTTA+AGG 0.359751 5.4:-48817007 MS.gene89511:CDS
TACAAACTTCTTTCAGAGTT+AGG 0.361224 5.4:-48817249 MS.gene89511:CDS
ACATTGGGAATTACAAATAC+TGG 0.371416 5.4:-48817192 MS.gene89511:CDS
TTTGACGGAAATGAAGTTGT+AGG 0.372591 5.4:+48817338 None:intergenic
GGAGGTGGAAAGGGTGAAGT+TGG 0.374363 5.4:-48817063 MS.gene89511:CDS
AACCCTACCAATTGTTTCAC+TGG 0.387990 5.4:+48817105 None:intergenic
ACAAATACTGGAAGCATGGC+TGG 0.392102 5.4:-48817180 MS.gene89511:CDS
GGTCCAAAGGTCTATGAATT+CGG 0.410970 5.4:-48817387 MS.gene89511:CDS
AGGTGGTTATTTGTTGGTTG+TGG 0.420306 5.4:-48816974 MS.gene89511:CDS
AAGCCAAAGAAAGGTAGAAA+TGG 0.439382 5.4:-48817132 MS.gene89511:CDS
CTCATTCTCATGTCTGTCAT+TGG 0.443342 5.4:+48817459 None:intergenic
ACTCTGAAAGAAGTTTGTAA+TGG 0.444238 5.4:+48817253 None:intergenic
TTTGAGAGTGTGACGGTGGA+AGG 0.447503 5.4:-48817084 MS.gene89511:CDS
GTATCGAATAAGTGGTTGAT+TGG 0.453731 5.4:+48817295 None:intergenic
TAGCCGAATTCATAGACCTT+TGG 0.462008 5.4:+48817384 None:intergenic
AATTCCCAATGTGACAGAAA+TGG 0.462642 5.4:+48817203 None:intergenic
ATGTCCATTTCTGTCACATT+GGG 0.462961 5.4:-48817207 MS.gene89511:CDS
ACGGTGGAAGGAGGTGGAAA+GGG 0.469710 5.4:-48817072 MS.gene89511:CDS
TTATTTGTTGGTTGTGGGAG+AGG 0.471909 5.4:-48816968 MS.gene89511:CDS
GGTGGTTATTTGTTGGTTGT+GGG 0.491601 5.4:-48816973 MS.gene89511:CDS
TGGAAATCCAGTGAAACAAT+TGG 0.493184 5.4:-48817112 MS.gene89511:CDS
GACGGTGGAAGGAGGTGGAA+AGG 0.507657 5.4:-48817073 MS.gene89511:CDS
CATGTCCATTTCTGTCACAT+TGG 0.509436 5.4:-48817208 MS.gene89511:CDS
ATGAGAGCTGATCCATCATC+AGG 0.511303 5.4:-48817441 MS.gene89511:CDS
GTAGAAACGGTAGGTTCTAC+CGG 0.511605 5.4:+48817413 None:intergenic
TACTCAATAAATATCACTGT+TGG 0.517582 5.4:-48816940 MS.gene89511:CDS
CTACCGGGATAGCCTGATGA+TGG 0.517596 5.4:+48817429 None:intergenic
TGGTTGATTGGTTGATGTGA+AGG 0.517616 5.4:+48817307 None:intergenic
TGAAGAAGGTGGTTATTTGT+TGG 0.528173 5.4:-48816980 MS.gene89511:CDS
CCTTTGGACCTGTGTAGAAA+CGG 0.540578 5.4:+48817400 None:intergenic
ATCCAGTGAAACAATTGGTA+GGG 0.540797 5.4:-48817107 MS.gene89511:CDS
GATCCATCATCAGGCTATCC+CGG 0.542828 5.4:-48817432 MS.gene89511:CDS
TTGTTTGTGAAGCCAAAGAA+AGG 0.545721 5.4:-48817141 MS.gene89511:CDS
AAGGTCTATGAATTCGGCTA+TGG 0.553533 5.4:-48817381 MS.gene89511:CDS
AATCCAGTGAAACAATTGGT+AGG 0.554451 5.4:-48817108 MS.gene89511:CDS
CAAATACTGGAAGCATGGCT+GGG 0.573487 5.4:-48817179 MS.gene89511:CDS
GAGAGTGTGACGGTGGAAGG+AGG 0.575492 5.4:-48817081 MS.gene89511:CDS
AGTGTGACGGTGGAAGGAGG+TGG 0.578214 5.4:-48817078 MS.gene89511:CDS
AATTACAAATACTGGAAGCA+TGG 0.582605 5.4:-48817184 MS.gene89511:CDS
CAATGTGACAGAAATGGACA+TGG 0.599069 5.4:+48817209 None:intergenic
GGTGTTAAGGTGATTGAAGA+AGG 0.603997 5.4:-48816994 MS.gene89511:CDS
CCGTTTCTACACAGGTCCAA+AGG 0.609130 5.4:-48817400 MS.gene89511:CDS
GGCTTCACAAACAACAACAC+AGG 0.609698 5.4:+48817150 None:intergenic
AGAACCTACCGTTTCTACAC+AGG 0.612051 5.4:-48817408 MS.gene89511:CDS
GTTAAGGTGATTGAAGAAGG+TGG 0.615321 5.4:-48816991 MS.gene89511:CDS
TGGACCTGTGTAGAAACGGT+AGG 0.625209 5.4:+48817404 None:intergenic
AGGGTTTGAGAGTGTGACGG+TGG 0.638866 5.4:-48817088 MS.gene89511:CDS
TAGAAACGGTAGGTTCTACC+GGG 0.648311 5.4:+48817414 None:intergenic
CTTAGTAGAGCAAATGAGAG+TGG 0.648348 5.4:-48817015 MS.gene89511:CDS
GGTAGGGTTTGAGAGTGTGA+CGG 0.652219 5.4:-48817091 MS.gene89511:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TACTCAATAAATATCACTGT+TGG - chr5.4:48817452-48817471 MS.gene89511:CDS 25.0%
AATTACAAATACTGGAAGCA+TGG - chr5.4:48817208-48817227 MS.gene89511:CDS 30.0%
ACATTGGGAATTACAAATAC+TGG - chr5.4:48817200-48817219 MS.gene89511:CDS 30.0%
TACAAACTTCTTTCAGAGTT+AGG - chr5.4:48817143-48817162 MS.gene89511:CDS 30.0%
TTTCCATTTCTACCTTTCTT+TGG + chr5.4:48817266-48817285 None:intergenic 30.0%
! ACTCTGAAAGAAGTTTGTAA+TGG + chr5.4:48817142-48817161 None:intergenic 30.0%
!!! GTGAAGGTTGTTATTTTTGA+CGG + chr5.4:48817072-48817091 None:intergenic 30.0%
AAGCCAAAGAAAGGTAGAAA+TGG - chr5.4:48817260-48817279 MS.gene89511:CDS 35.0%
AATCCAGTGAAACAATTGGT+AGG - chr5.4:48817284-48817303 MS.gene89511:CDS 35.0%
AATTCCCAATGTGACAGAAA+TGG + chr5.4:48817192-48817211 None:intergenic 35.0%
ATCCAGTGAAACAATTGGTA+GGG - chr5.4:48817285-48817304 MS.gene89511:CDS 35.0%
ATGTCCATTTCTGTCACATT+GGG - chr5.4:48817185-48817204 MS.gene89511:CDS 35.0%
GTATCGAATAAGTGGTTGAT+TGG + chr5.4:48817100-48817119 None:intergenic 35.0%
TGAAGAAGGTGGTTATTTGT+TGG - chr5.4:48817412-48817431 MS.gene89511:CDS 35.0%
TGGAAATCCAGTGAAACAAT+TGG - chr5.4:48817280-48817299 MS.gene89511:CDS 35.0%
TTGTTTGTGAAGCCAAAGAA+AGG - chr5.4:48817251-48817270 MS.gene89511:CDS 35.0%
TTTGACGGAAATGAAGTTGT+AGG + chr5.4:48817057-48817076 None:intergenic 35.0%
!!! CTTTTCGAGTATCGAATAAG+TGG + chr5.4:48817108-48817127 None:intergenic 35.0%
AACCCTACCAATTGTTTCAC+TGG + chr5.4:48817290-48817309 None:intergenic 40.0%
AAGGTCTATGAATTCGGCTA+TGG - chr5.4:48817011-48817030 MS.gene89511:CDS 40.0%
CAATGTGACAGAAATGGACA+TGG + chr5.4:48817186-48817205 None:intergenic 40.0%
CATGTCCATTTCTGTCACAT+TGG - chr5.4:48817184-48817203 MS.gene89511:CDS 40.0%
CTTAGTAGAGCAAATGAGAG+TGG - chr5.4:48817377-48817396 MS.gene89511:CDS 40.0%
GGTCCAAAGGTCTATGAATT+CGG - chr5.4:48817005-48817024 MS.gene89511:CDS 40.0%
GGTGTTAAGGTGATTGAAGA+AGG - chr5.4:48817398-48817417 MS.gene89511:CDS 40.0%
GTTAAGGTGATTGAAGAAGG+TGG - chr5.4:48817401-48817420 MS.gene89511:CDS 40.0%
TAGCCGAATTCATAGACCTT+TGG + chr5.4:48817011-48817030 None:intergenic 40.0%
! AGCAAATGAGAGTGGTGTTA+AGG - chr5.4:48817385-48817404 MS.gene89511:CDS 40.0%
! AGGTGGTTATTTGTTGGTTG+TGG - chr5.4:48817418-48817437 MS.gene89511:CDS 40.0%
! GGTGGTTATTTGTTGGTTGT+GGG - chr5.4:48817419-48817438 MS.gene89511:CDS 40.0%
! TGGTTGATTGGTTGATGTGA+AGG + chr5.4:48817088-48817107 None:intergenic 40.0%
! TTATTTGTTGGTTGTGGGAG+AGG - chr5.4:48817424-48817443 MS.gene89511:CDS 40.0%
AGAACCTACCGTTTCTACAC+AGG - chr5.4:48816984-48817003 MS.gene89511:CDS 45.0%
ATGAGAGCTGATCCATCATC+AGG - chr5.4:48816951-48816970 MS.gene89511:CDS 45.0%
CCTTTGGACCTGTGTAGAAA+CGG + chr5.4:48816995-48817014 None:intergenic 45.0%
GGCTTCACAAACAACAACAC+AGG + chr5.4:48817245-48817264 None:intergenic 45.0%
GTAGAAACGGTAGGTTCTAC+CGG + chr5.4:48816982-48817001 None:intergenic 45.0%
TAGAAACGGTAGGTTCTACC+GGG + chr5.4:48816981-48817000 None:intergenic 45.0%
! ACAAATACTGGAAGCATGGC+TGG - chr5.4:48817212-48817231 MS.gene89511:CDS 45.0%
! CAAATACTGGAAGCATGGCT+GGG - chr5.4:48817213-48817232 MS.gene89511:CDS 45.0%
CCGTTTCTACACAGGTCCAA+AGG - chr5.4:48816992-48817011 MS.gene89511:CDS 50.0%
GATCCATCATCAGGCTATCC+CGG - chr5.4:48816960-48816979 MS.gene89511:CDS 50.0%
TGGACCTGTGTAGAAACGGT+AGG + chr5.4:48816991-48817010 None:intergenic 50.0%
! GGTAGGGTTTGAGAGTGTGA+CGG - chr5.4:48817301-48817320 MS.gene89511:CDS 50.0%
! TTTGAGAGTGTGACGGTGGA+AGG - chr5.4:48817308-48817327 MS.gene89511:CDS 50.0%
ACGGTGGAAGGAGGTGGAAA+GGG - chr5.4:48817320-48817339 MS.gene89511:CDS 55.0%
CTACCGGGATAGCCTGATGA+TGG + chr5.4:48816966-48816985 None:intergenic 55.0%
GGAGGTGGAAAGGGTGAAGT+TGG - chr5.4:48817329-48817348 MS.gene89511:CDS 55.0%
! AGGGTTTGAGAGTGTGACGG+TGG - chr5.4:48817304-48817323 MS.gene89511:CDS 55.0%
AGTGTGACGGTGGAAGGAGG+TGG - chr5.4:48817314-48817333 MS.gene89511:CDS 60.0%
GACGGTGGAAGGAGGTGGAA+AGG - chr5.4:48817319-48817338 MS.gene89511:CDS 60.0%
! GAGAGTGTGACGGTGGAAGG+AGG - chr5.4:48817311-48817330 MS.gene89511:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 48816936 48817478 48816936 ID=MS.gene89511
chr5.4 mRNA 48816936 48817478 48816936 ID=MS.gene89511.t1;Parent=MS.gene89511
chr5.4 exon 48816936 48817478 48816936 ID=MS.gene89511.t1.exon1;Parent=MS.gene89511.t1
chr5.4 CDS 48816936 48817478 48816936 ID=cds.MS.gene89511.t1;Parent=MS.gene89511.t1
Gene Sequence

>MS.gene89511

ATGACAGACATGAGAATGAGAGCTGATCCATCATCAGGCTATCCCGGTAGAACCTACCGTTTCTACACAGGTCCAAAGGTCTATGAATTCGGCTATGGTCTAAGCTACTCAAATTATTCCTACAACTTCATTTCCGTCAAAAATAACAACCTTCACATCAACCAATCAACCACTTATTCGATACTCGAAAAGTCTCATACAATCCATTACAAACTTCTTTCAGAGTTAGGCGAAAAAACATGTAAAACCATGTCCATTTCTGTCACATTGGGAATTACAAATACTGGAAGCATGGCTGGGAAACATCCTGTGTTGTTGTTTGTGAAGCCAAAGAAAGGTAGAAATGGAAATCCAGTGAAACAATTGGTAGGGTTTGAGAGTGTGACGGTGGAAGGAGGTGGAAAGGGTGAAGTTGGATTTGAAGTTAGTGTTTGTGAGCATCTTAGTAGAGCAAATGAGAGTGGTGTTAAGGTGATTGAAGAAGGTGGTTATTTGTTGGTTGTGGGAGAGGAAGAGTACTCAATAAATATCACTGTTGGATAA

Protein sequence

>MS.gene89511.t1

MTDMRMRADPSSGYPGRTYRFYTGPKVYEFGYGLSYSNYSYNFISVKNNNLHINQSTTYSILEKSHTIHYKLLSELGEKTCKTMSISVTLGITNTGSMAGKHPVLLFVKPKKGRNGNPVKQLVGFESVTVEGGGKGEVGFEVSVCEHLSRANESGVKVIEEGGYLLVVGEEEYSINITVG