AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92819


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92819.t1 MTR_7g010150 100.000 120 0 0 1 120 1 120 4.69e-83 238
MS.gene92819.t1 MTR_8g467460 99.160 119 1 0 1 119 1 119 2.73e-81 234
MS.gene92819.t1 MTR_5g040570 96.667 120 4 0 1 120 1 120 2.85e-80 231
MS.gene92819.t1 MTR_7g011050 63.717 113 25 1 1 113 1 97 5.56e-44 139
MS.gene92819.t1 MTR_8g019410 47.863 117 23 2 4 119 47 126 1.49e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92819.t1 AT1G26880 90.833 120 11 0 1 120 1 120 2.57e-75 219
MS.gene92819.t1 AT1G69620 91.597 119 10 0 1 119 1 119 9.90e-75 217
MS.gene92819.t1 AT3G28900 88.333 120 14 0 1 120 1 120 4.98e-73 213
MS.gene92819.t1 AT1G26880 91.304 92 8 0 1 92 1 92 6.96e-58 174

Find 31 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACCGCCGCACTGTTAAC+AGG 0.305052 7.1:+85340582 MS.gene92819:CDS
TGGATCCTCTTTCCAGTAAC+AGG 0.314412 7.1:-85339319 None:intergenic
GGACCTAAGTGTCCTGTTAC+TGG 0.331250 7.1:+85339307 MS.gene92819:CDS
CGCACTGTTAACAGGGCTTA+TGG 0.356563 7.1:+85340590 MS.gene92819:CDS
AACACGATGCTGGTTGGATT+TGG 0.389836 7.1:-85339085 None:intergenic
TTAACAGTGCGGCGGTTCCT+AGG 0.468395 7.1:-85340578 None:intergenic
CCTTTCCCTAACAGCTCCTC+CGG 0.470814 7.1:-85340624 None:intergenic
TTTATCCGGAGGAGCTGTTA+GGG 0.501294 7.1:+85340619 MS.gene92819:CDS
TTTCCAGTAACAGGACACTT+AGG 0.509665 7.1:-85339310 None:intergenic
ACGATGCTGGTTGGATTTGG+TGG 0.532546 7.1:-85339082 None:intergenic
CATCGTGTTGTTAAGACACC+TGG 0.536949 7.1:+85339099 MS.gene92819:CDS
AGTGTCCTGTTACTGGAAAG+AGG 0.543803 7.1:+85339314 MS.gene92819:CDS
CTTAACAACACGATGCTGGT+TGG 0.545517 7.1:-85339091 None:intergenic
GTTACTGGAAAGAGGATCCA+AGG 0.571456 7.1:+85339322 MS.gene92819:CDS
ACAACTAAGAAGAGAGCTAG+TGG 0.577057 7.1:+85339286 MS.gene92819:CDS
GTGTCTTAACAACACGATGC+TGG 0.578379 7.1:-85339095 None:intergenic
TGGCATAGCTGTGTCTCCTA+CGG 0.580308 7.1:-85339062 None:intergenic
GCTCTTCAACAAGGAAAGCA+CGG 0.587996 7.1:-85340738 None:intergenic
CCGGAGGAGCTGTTAGGGAA+AGG 0.595594 7.1:+85340624 MS.gene92819:CDS
GAACCGCCGCACTGTTAACA+GGG 0.611783 7.1:+85340583 MS.gene92819:CDS
CTAGACCTCTTGTATTCAGT+AGG 0.617053 7.1:-85340551 None:intergenic
TTACTGGAAAGAGGATCCAA+GGG 0.629653 7.1:+85339323 MS.gene92819:CDS
TGAGACCTACTGAATACAAG+AGG 0.631121 7.1:+85340546 MS.gene92819:CDS
ACAAGAGGTCTAGATTGCCT+AGG 0.636078 7.1:+85340561 MS.gene92819:CDS
TATTCAGTAGGTCTCAAGTG+TGG 0.667813 7.1:-85340539 None:intergenic
AAGCCCTGTTAACAGTGCGG+CGG 0.691394 7.1:-85340586 None:intergenic
ACTGTTAACAGGGCTTATGG+AGG 0.693206 7.1:+85340593 MS.gene92819:CDS
TTCAGAGACTCACATACCGT+AGG 0.721913 7.1:+85339046 MS.gene92819:CDS
CATAAGCCCTGTTAACAGTG+CGG 0.730145 7.1:-85340589 None:intergenic
TACTGGAAAGAGGATCCAAG+GGG 0.738216 7.1:+85339324 MS.gene92819:CDS
GTATGTGAGTCTCTGAACCA+TGG 0.739024 7.1:-85339040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAATCAATTATTTG+TGG + chr7.1:85339610-85339629 MS.gene92819:intron 10.0%
!! ATTTGAAAATTGAAAATATT+AGG - chr7.1:85339694-85339713 None:intergenic 10.0%
!!! TTTTTTAGAAATTAATATGT+TGG + chr7.1:85339368-85339387 MS.gene92819:intron 10.0%
!! TAACAAATCTTCAATAAAAA+TGG - chr7.1:85340024-85340043 None:intergenic 15.0%
!! TTTGCAATTATTAATGTTAA+TGG + chr7.1:85339575-85339594 MS.gene92819:intron 15.0%
!!! TTTGATTAATTTGTGAAAAT+GGG + chr7.1:85339228-85339247 MS.gene92819:intron 15.0%
!!! TTTTGATTAATTTGTGAAAA+TGG + chr7.1:85339227-85339246 MS.gene92819:intron 15.0%
!! GAATGAAAATTAAAAGTAGT+AGG - chr7.1:85340398-85340417 None:intergenic 20.0%
!!! TTAATACTACAATTTTGTGA+GGG - chr7.1:85340184-85340203 None:intergenic 20.0%
!!! TTAATATGTTGGTTATACAT+AGG + chr7.1:85339379-85339398 MS.gene92819:intron 20.0%
!!! TTTAATACTACAATTTTGTG+AGG - chr7.1:85340185-85340204 None:intergenic 20.0%
! AAACTATCTCTTCTGTAATA+GGG - chr7.1:85339882-85339901 None:intergenic 25.0%
! AATGTTCTTACTTCAATGTT+GGG + chr7.1:85339644-85339663 MS.gene92819:intron 25.0%
! AGATACCCTTTCTAAATTAT+TGG + chr7.1:85340204-85340223 MS.gene92819:intron 25.0%
! AGTCACCAATAATTTAGAAA+GGG - chr7.1:85340212-85340231 None:intergenic 25.0%
! ATCTATCATGTTTCAAACAT+AGG - chr7.1:85340048-85340067 None:intergenic 25.0%
! GAAAATTAAAAGTAGTAGGT+AGG - chr7.1:85340394-85340413 None:intergenic 25.0%
! GACTTGTATTATTCATCAAT+TGG + chr7.1:85339495-85339514 MS.gene92819:intron 25.0%
! GATATATGTGAGTTTATGAT+AGG + chr7.1:85339473-85339492 MS.gene92819:intron 25.0%
! GCATCATTGAAATTATACAA+TGG - chr7.1:85340297-85340316 None:intergenic 25.0%
! TGTAAAATAGGTGGAATATT+AGG + chr7.1:85339540-85339559 MS.gene92819:intron 25.0%
! TTATCATATTTGTTCTGTTC+TGG + chr7.1:85340500-85340519 MS.gene92819:intron 25.0%
! TTGAATTCATCTATGTTAGT+TGG - chr7.1:85339724-85339743 None:intergenic 25.0%
! TTGTCTGTGTTATGTAAAAT+AGG + chr7.1:85339528-85339547 MS.gene92819:intron 25.0%
AAGTGTCAAGATTATATGCA+GGG - chr7.1:85340370-85340389 None:intergenic 30.0%
CAAACTATCTCTTCTGTAAT+AGG - chr7.1:85339883-85339902 None:intergenic 30.0%
CAACACAATATTCAATCAGA+TGG - chr7.1:85339852-85339871 None:intergenic 30.0%
CAGTCACCAATAATTTAGAA+AGG - chr7.1:85340213-85340232 None:intergenic 30.0%
GAATGTTCTTACTTCAATGT+TGG + chr7.1:85339643-85339662 MS.gene92819:intron 30.0%
TCTGTGTTATGTAAAATAGG+TGG + chr7.1:85339531-85339550 MS.gene92819:intron 30.0%
! AAAATTGATGATAGCGTCAA+AGG - chr7.1:85340094-85340113 None:intergenic 30.0%
! AAAGTGTCAAGATTATATGC+AGG - chr7.1:85340371-85340390 None:intergenic 30.0%
!!! AAATGGGTTTGTGTTGTTTT+AGG + chr7.1:85339244-85339263 MS.gene92819:intron 30.0%
!!! CTATTTTTTGCTCTTCAACA+AGG - chr7.1:85340750-85340769 None:intergenic 30.0%
!!! CTTGCTTTTCTTTTGTTTTC+TGG - chr7.1:85340792-85340811 None:intergenic 30.0%
CAATTCTACAACATTGTGGA+AGG + chr7.1:85340316-85340335 MS.gene92819:intron 35.0%
GATGCAATTCTACAACATTG+TGG + chr7.1:85340312-85340331 MS.gene92819:intron 35.0%
TCATATAGATTCATACCCCT+TGG - chr7.1:85339342-85339361 None:intergenic 35.0%
TGACAAAGAAGAACATTACC+AGG - chr7.1:85339120-85339139 None:intergenic 35.0%
TTTGTTTGTACATGATGCTG+TGG + chr7.1:85339437-85339456 MS.gene92819:intron 35.0%
! CAATTTTGTGAGGGACAATA+AGG - chr7.1:85340175-85340194 None:intergenic 35.0%
!!! TGTTGTTTTAGGTGGAAAGT+TGG + chr7.1:85339255-85339274 MS.gene92819:intron 35.0%
AAACTTAGACGCTGTGTGAT+AGG - chr7.1:85340271-85340290 None:intergenic 40.0%
ACAACTAAGAAGAGAGCTAG+TGG + chr7.1:85339286-85339305 MS.gene92819:CDS 40.0%
ATAGCGTCAAAGGTCATAGT+TGG - chr7.1:85340084-85340103 None:intergenic 40.0%
CTAGACCTCTTGTATTCAGT+AGG - chr7.1:85340554-85340573 None:intergenic 40.0%
CTTCACAAACACAGCTGAAT+TGG - chr7.1:85339965-85339984 None:intergenic 40.0%
TATTCAGTAGGTCTCAAGTG+TGG - chr7.1:85340542-85340561 None:intergenic 40.0%
TGAGACCTACTGAATACAAG+AGG + chr7.1:85340546-85340565 MS.gene92819:CDS 40.0%
TTACTGGAAAGAGGATCCAA+GGG + chr7.1:85339323-85339342 MS.gene92819:CDS 40.0%
TTTCCAGTAACAGGACACTT+AGG - chr7.1:85339313-85339332 None:intergenic 40.0%
TTTGTGAGGGACAATAAGGA+CGG - chr7.1:85340171-85340190 None:intergenic 40.0%
!!! GCTTATGGAGGTGTTTTATC+CGG + chr7.1:85340605-85340624 MS.gene92819:CDS 40.0%
!!! TCATTGCTTTTTCTCCCTGA+TGG + chr7.1:85340425-85340444 MS.gene92819:intron 40.0%
!!! TGGGTTTGTGTTGTTTTAGG+TGG + chr7.1:85339247-85339266 MS.gene92819:intron 40.0%
ACAAGAGGTCTAGATTGCCT+AGG + chr7.1:85340561-85340580 MS.gene92819:CDS 45.0%
ACTGTTAACAGGGCTTATGG+AGG + chr7.1:85340593-85340612 MS.gene92819:CDS 45.0%
AGTGCATGTCATGACCATCA+GGG - chr7.1:85340442-85340461 None:intergenic 45.0%
AGTGTCCTGTTACTGGAAAG+AGG + chr7.1:85339314-85339333 MS.gene92819:CDS 45.0%
CATAAGCCCTGTTAACAGTG+CGG - chr7.1:85340592-85340611 None:intergenic 45.0%
CTTAACAACACGATGCTGGT+TGG - chr7.1:85339094-85339113 None:intergenic 45.0%
GCTCTTCAACAAGGAAAGCA+CGG - chr7.1:85340741-85340760 None:intergenic 45.0%
GTGTCTTAACAACACGATGC+TGG - chr7.1:85339098-85339117 None:intergenic 45.0%
GTTACTGGAAAGAGGATCCA+AGG + chr7.1:85339322-85339341 MS.gene92819:CDS 45.0%
TACTGGAAAGAGGATCCAAG+GGG + chr7.1:85339324-85339343 MS.gene92819:CDS 45.0%
TGGATCCTCTTTCCAGTAAC+AGG - chr7.1:85339322-85339341 None:intergenic 45.0%
TTCAGAGACTCACATACCGT+AGG + chr7.1:85339046-85339065 MS.gene92819:CDS 45.0%
TTTATCCGGAGGAGCTGTTA+GGG + chr7.1:85340619-85340638 MS.gene92819:CDS 45.0%
! AACACGATGCTGGTTGGATT+TGG - chr7.1:85339088-85339107 None:intergenic 45.0%
! CATCGTGTTGTTAAGACACC+TGG + chr7.1:85339099-85339118 MS.gene92819:CDS 45.0%
! TTTTATCCGGAGGAGCTGTT+AGG + chr7.1:85340618-85340637 MS.gene92819:CDS 45.0%
!!! TATGGAGGTGTTTTATCCGG+AGG + chr7.1:85340608-85340627 MS.gene92819:CDS 45.0%
CGCACTGTTAACAGGGCTTA+TGG + chr7.1:85340590-85340609 MS.gene92819:CDS 50.0%
GAGTGCATGTCATGACCATC+AGG - chr7.1:85340443-85340462 None:intergenic 50.0%
GGACCTAAGTGTCCTGTTAC+TGG + chr7.1:85339307-85339326 MS.gene92819:CDS 50.0%
GTCTCAAGTGTGGAATCTGC+AGG - chr7.1:85340532-85340551 None:intergenic 50.0%
TGGCATAGCTGTGTCTCCTA+CGG - chr7.1:85339065-85339084 None:intergenic 50.0%
! ACGATGCTGGTTGGATTTGG+TGG - chr7.1:85339085-85339104 None:intergenic 50.0%
AAGCCCTGTTAACAGTGCGG+CGG - chr7.1:85340589-85340608 None:intergenic 55.0%
CCTTTCCCTAACAGCTCCTC+CGG - chr7.1:85340627-85340646 None:intergenic 55.0%
GAACCGCCGCACTGTTAACA+GGG + chr7.1:85340583-85340602 MS.gene92819:CDS 55.0%
TTAACAGTGCGGCGGTTCCT+AGG - chr7.1:85340581-85340600 None:intergenic 55.0%
CCGGAGGAGCTGTTAGGGAA+AGG + chr7.1:85340624-85340643 MS.gene92819:CDS 60.0%
GGAACCGCCGCACTGTTAAC+AGG + chr7.1:85340582-85340601 MS.gene92819:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 85339042 85340825 85339042 ID=MS.gene92819
chr7.1 mRNA 85339042 85340825 85339042 ID=MS.gene92819.t1;Parent=MS.gene92819
chr7.1 exon 85339042 85339120 85339042 ID=MS.gene92819.t1.exon1;Parent=MS.gene92819.t1
chr7.1 CDS 85339042 85339120 85339042 ID=cds.MS.gene92819.t1;Parent=MS.gene92819.t1
chr7.1 exon 85339266 85339345 85339266 ID=MS.gene92819.t1.exon2;Parent=MS.gene92819.t1
chr7.1 CDS 85339266 85339345 85339266 ID=cds.MS.gene92819.t1;Parent=MS.gene92819.t1
chr7.1 exon 85340536 85340645 85340536 ID=MS.gene92819.t1.exon3;Parent=MS.gene92819.t1
chr7.1 CDS 85340536 85340645 85340536 ID=cds.MS.gene92819.t1;Parent=MS.gene92819.t1
chr7.1 exon 85340732 85340825 85340732 ID=MS.gene92819.t1.exon4;Parent=MS.gene92819.t1
chr7.1 CDS 85340732 85340825 85340732 ID=cds.MS.gene92819.t1;Parent=MS.gene92819.t1
Gene Sequence

>MS.gene92819

ATGGTTCAGAGACTCACATACCGTAGGAGACACAGCTATGCCACCAAATCCAACCAGCATCGTGTTGTTAAGACACCTGGTAATGTTCTTCTTTGTCAAATTGTTCTGTTTTTTCTTGTTCTAATCTATGTTATGATGTTTTTGATTTTTTCTTAAAAATTGCATTTTTTTGTTGTTGAAGTGTTTTTTGATTAATTTGTGAAAATGGGTTTGTGTTGTTTTAGGTGGAAAGTTGGTTTATCAGACAACTAAGAAGAGAGCTAGTGGACCTAAGTGTCCTGTTACTGGAAAGAGGATCCAAGGGGTATGAATCTATATGATATCATTTTTTTAGAAATTAATATGTTGGTTATACATAGGATCTGTGATTCATTGTGTTGATTCTATTTTTTTTGTTTGTTTGTACATGATGCTGTGGAAAAATGTAGATTGATATATGTGAGTTTATGATAGGACTTGTATTATTCATCAATTGGTTGTGTGAATTTGTCTGTGTTATGTAAAATAGGTGGAATATTAGGATTGTTTTGTGTTTTGCAATTATTAATGTTAATGGAATGCTGAGCTTATTATAAAATCAATTATTTGTGGTTTATTGAGCGAATGTTCTTACTTCAATGTTGGGTTTACTGTTTGATTGTTTGAAAATCCTAATATTTTCAATTTTCAAATCTATATTCCAACTAACATAGATGAATTCAATCAAATCATTTGTCTTGTATTTTGATTAATGTTAATCGCTGAGCATATTATGAAATCAATTACTGTGTTTTTTTTAGTTAGCTTTTACTGTCTTTTATGCATGCTCCATCTGATTGAATATTGTGTTGTTTACGTCCCTATTACAGAAGAGATAGTTTGTTTTCTGCTTTAATTATGTGATGATTCAGTTTTTTTGAAGACGTAAATAACTTAATTCACCAATTCAGCTGTGTTTGTGAAGTGAATTAGTATAGTCTGTCTGTTTTTTTTGTTAAGTCCATTTTTATTGAAGATTTGTTAGCCTATGTTTGAAACATGATAGATATAATAATCATTTCCAACTATGACCTTTGACGCTATCATCAATTTTATAATGACAAAAATTAGATAGTTAGACTTAGATGCAATTCTTTTGAGATTGACACCGTCCTTATTGTCCCTCACAAAATTGTAGTATTAAAGATACCCTTTCTAAATTATTGGTGACTGTTTTTCTTATTTAGTTACAAACAACTAGAGCAGTGCCTATCACACAGCGTCTAAGTTTTGTCCATTGTATAATTTCAATGATGCAATTCTACAACATTGTGGAAGGAACATACAATGAATGTATTACTGTGTTACCCTGCATATAATCTTGACACTTTCCTACCTACTACTTTTAATTTTCATTCAAAGATTTCATTGCTTTTTCTCCCTGATGGTCATGACATGCACTCTTTATCTTTAGTTGCAAGTTGATCAATGTGTGTTCTCTTATCATATTTGTTCTGTTCTGGACTTGACCTGCAGATTCCACACTTGAGACCTACTGAATACAAGAGGTCTAGATTGCCTAGGAACCGCCGCACTGTTAACAGGGCTTATGGAGGTGTTTTATCCGGAGGAGCTGTTAGGGAAAGGTATGTTGCTCATAACATAATTACAGATTTATATTCAAAATTTTGAATATTGTAGTAAATTTTAATGCTCTTCAATCTTTGTGCAGAATTATCCGTGCTTTCCTTGTTGAAGAGCAAAAAATAGTTAAGAAAGTTTTGAAGATCCAGAAAACAAAAGAAAAGCAAGCAGCTAAGAGTTAA

Protein sequence

>MS.gene92819.t1

MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEEQKIVKKVLKIQKTKEKQAAKS