Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93418.t1 | XP_013469937.1 | 86.7 | 547 | 71 | 2 | 1 | 547 | 1 | 545 | 2.10E-125 | 459.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93418.t1 | G8A0T5 | 86.7 | 547 | 71 | 2 | 1 | 547 | 1 | 545 | 1.5e-125 | 459.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene93418 | 0.85115 | 1.07E-60 | -1.69E-46 |
| MS.gene049159 | MS.gene93418 | 0.836118 | 1.13E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene93418 | 0.812641 | 3.83E-51 | -1.69E-46 |
| MS.gene049368 | MS.gene93418 | 0.800396 | 1.47E-48 | -1.69E-46 |
| MS.gene049404 | MS.gene93418 | 0.825423 | 4.73E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene93418 | 0.822696 | 2.06E-53 | -1.69E-46 |
| MS.gene049807 | MS.gene93418 | 0.839078 | 1.96E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene93418 | 0.820582 | 6.36E-53 | -1.69E-46 |
| MS.gene049983 | MS.gene93418 | 0.827337 | 1.65E-54 | -1.69E-46 |
| MS.gene050273 | MS.gene93418 | 0.812954 | 3.27E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene93418 | 0.810629 | 1.05E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene93418 | 0.850806 | 1.34E-60 | -1.69E-46 |
| MS.gene050737 | MS.gene93418 | 0.823367 | 1.44E-53 | -1.69E-46 |
| MS.gene050759 | MS.gene93418 | 0.837273 | 5.73E-57 | -1.69E-46 |
| MS.gene050941 | MS.gene93418 | 0.813913 | 2.01E-51 | -1.69E-46 |
| MS.gene050962 | MS.gene93418 | 0.824993 | 5.97E-54 | -1.69E-46 |
| MS.gene051198 | MS.gene93418 | 0.847524 | 1.10E-59 | -1.69E-46 |
| MS.gene051247 | MS.gene93418 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene93418 | 0.817619 | 3.00E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene93418 | 0.810112 | 1.36E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene93418 | 0.823686 | 1.21E-53 | -1.69E-46 |
| MS.gene051800 | MS.gene93418 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene052037 | MS.gene93418 | 0.805797 | 1.12E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene93418 | 0.84479 | 6.08E-59 | -1.69E-46 |
| MS.gene052459 | MS.gene93418 | 0.821286 | 4.38E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene93418 | 0.825868 | 3.71E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene93418 | 0.879602 | 1.09E-69 | -1.69E-46 |
| MS.gene052519 | MS.gene93418 | 0.814687 | 1.36E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene93418 | 0.816246 | 6.10E-52 | -1.69E-46 |
| MS.gene052611 | MS.gene93418 | 0.804924 | 1.71E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene93418 | 0.829119 | 6.15E-55 | -1.69E-46 |
| MS.gene052896 | MS.gene93418 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene93418 | 0.809997 | 1.43E-50 | -1.69E-46 |
| MS.gene053079 | MS.gene93418 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene93418 | 0.822269 | 2.59E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene93418 | 0.808147 | 3.57E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene93418 | 0.807928 | 3.98E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene93418 | 0.820552 | 6.46E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene93418 | 0.805461 | 1.32E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene93418 | 0.81991 | 9.06E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene93418 | 0.859206 | 4.86E-63 | -1.69E-46 |
| MS.gene055465 | MS.gene93418 | 0.823167 | 1.60E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene93418 | 0.843117 | 1.71E-58 | -1.69E-46 |
| MS.gene055468 | MS.gene93418 | 0.822149 | 2.76E-53 | -1.69E-46 |
| MS.gene055469 | MS.gene93418 | 0.816988 | 4.16E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene93418 | 0.822146 | 2.77E-53 | -1.69E-46 |
| MS.gene055580 | MS.gene93418 | -0.818461 | 1.94E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene93418 | 0.825019 | 5.89E-54 | -1.69E-46 |
| MS.gene056043 | MS.gene93418 | -0.80633 | 8.67E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene93418 | 0.817273 | 3.59E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene93418 | 0.841583 | 4.35E-58 | -1.69E-46 |
| MS.gene056366 | MS.gene93418 | 0.801346 | 9.40E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene93418 | 0.810545 | 1.09E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene93418 | 0.809426 | 1.90E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene93418 | 0.838005 | 3.72E-57 | -1.69E-46 |
| MS.gene05686 | MS.gene93418 | 0.814007 | 1.92E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene93418 | 0.825823 | 3.80E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene93418 | 0.815892 | 7.33E-52 | -1.69E-46 |
| MS.gene057294 | MS.gene93418 | 0.806926 | 6.49E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene93418 | 0.819104 | 1.38E-52 | -1.69E-46 |
| MS.gene057446 | MS.gene93418 | 0.807434 | 5.06E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene93418 | 0.809342 | 1.98E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene93418 | 0.82799 | 1.15E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene93418 | 0.807938 | 3.96E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene93418 | 0.817535 | 3.14E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene93418 | 0.854428 | 1.24E-61 | -1.69E-46 |
| MS.gene058339 | MS.gene93418 | 0.808843 | 2.54E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene93418 | 0.825145 | 5.50E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene93418 | 0.829721 | 4.39E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene93418 | 0.845303 | 4.42E-59 | -1.69E-46 |
| MS.gene058979 | MS.gene93418 | 0.824758 | 6.79E-54 | -1.69E-46 |
| MS.gene059170 | MS.gene93418 | 0.813285 | 2.77E-51 | -1.69E-46 |
| MS.gene059570 | MS.gene93418 | 0.804709 | 1.90E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene93418 | 0.852604 | 4.14E-61 | -1.69E-46 |
| MS.gene059937 | MS.gene93418 | 0.80199 | 6.94E-49 | -1.69E-46 |
| MS.gene059965 | MS.gene93418 | 0.809083 | 2.25E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene93418 | 0.806473 | 8.09E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene93418 | 0.852353 | 4.88E-61 | -1.69E-46 |
| MS.gene060587 | MS.gene93418 | 0.801386 | 9.23E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene93418 | 0.815753 | 7.87E-52 | -1.69E-46 |
| MS.gene060935 | MS.gene93418 | 0.805088 | 1.58E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene93418 | 0.841334 | 5.06E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93418.t1 | MTR_1g105890 | 92.687 | 547 | 38 | 2 | 1 | 547 | 1 | 545 | 0.0 | 885 |
| MS.gene93418.t1 | MTR_5g005430 | 41.340 | 612 | 238 | 21 | 1 | 545 | 1 | 558 | 2.86e-87 | 280 |
| MS.gene93418.t1 | MTR_4g097790 | 46.053 | 76 | 31 | 4 | 1 | 74 | 1 | 68 | 2.22e-17 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93418.t1 | AT4G33740 | 38.504 | 361 | 181 | 10 | 7 | 354 | 5 | 337 | 1.05e-32 | 131 |
| MS.gene93418.t1 | AT4G33740 | 38.504 | 361 | 181 | 10 | 7 | 354 | 5 | 337 | 1.05e-32 | 131 |
| MS.gene93418.t1 | AT4G33740 | 38.504 | 361 | 181 | 10 | 7 | 354 | 5 | 337 | 1.05e-32 | 131 |
| MS.gene93418.t1 | AT4G33740 | 38.504 | 361 | 181 | 10 | 7 | 354 | 5 | 337 | 1.05e-32 | 131 |
| MS.gene93418.t1 | AT4G33740 | 38.504 | 361 | 181 | 10 | 7 | 354 | 5 | 337 | 1.05e-32 | 131 |
| MS.gene93418.t1 | AT4G37820 | 50.000 | 92 | 40 | 5 | 1 | 91 | 1 | 87 | 3.87e-19 | 91.3 |
| MS.gene93418.t1 | AT4G37820 | 50.000 | 92 | 40 | 5 | 1 | 91 | 1 | 87 | 3.87e-19 | 91.3 |
Find 98 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTAACGTGTCCAGTAATT+TGG | 0.180302 | 1.1:-75989857 | MS.gene93418:CDS |
| GTTTCTGTTAAACCTGAAAA+TGG | 0.226292 | 1.1:-75989961 | MS.gene93418:CDS |
| TGTTGCTTGGTGTCTGCTTC+TGG | 0.245511 | 1.1:-75991085 | MS.gene93418:CDS |
| TAGCACTGAAACAAGTAATT+TGG | 0.256562 | 1.1:-75990439 | MS.gene93418:CDS |
| CGCATTTCTATCCAAATTAC+TGG | 0.276741 | 1.1:+75989846 | None:intergenic |
| CTAAGTTAACCATGATAAAA+AGG | 0.287367 | 1.1:-75991172 | None:intergenic |
| TTGGCTATCGGGGTTTGTTT+TGG | 0.298402 | 1.1:+75990224 | None:intergenic |
| GGTAGAAAGGACCTTCTTCC+GGG | 0.327131 | 1.1:-75990960 | MS.gene93418:CDS |
| TCAAAACATACATGAGGATA+TGG | 0.336233 | 1.1:-75989620 | MS.gene93418:CDS |
| TGGTAGAAAGGACCTTCTTC+CGG | 0.348772 | 1.1:-75990961 | MS.gene93418:CDS |
| CTTAAATCCAATTGGCTATC+GGG | 0.355327 | 1.1:+75990213 | None:intergenic |
| ACAGTTAATACAACAATTAC+TGG | 0.363960 | 1.1:-75990063 | MS.gene93418:CDS |
| AGACAAGGAGGATGAGGAAA+AGG | 0.369456 | 1.1:-75990559 | MS.gene93418:CDS |
| ATCATTCTGATGAAAGTAAC+AGG | 0.386411 | 1.1:-75990359 | MS.gene93418:CDS |
| ACACGACACTATTGATTCTT+CGG | 0.386613 | 1.1:-75989647 | MS.gene93418:CDS |
| GACAAGGAGGATGAGGAAAA+GGG | 0.393398 | 1.1:-75990558 | MS.gene93418:CDS |
| TTTCCTTCATTTCTAATATC+CGG | 0.393946 | 1.1:+75989568 | None:intergenic |
| GATCTTTGATTCCTACTACT+TGG | 0.415150 | 1.1:+75991146 | None:intergenic |
| CTTCAAGCTATTCTGTTGCT+TGG | 0.419009 | 1.1:-75991098 | MS.gene93418:CDS |
| TCAAAATGATTCTGATTTGA+AGG | 0.420588 | 1.1:-75990334 | MS.gene93418:CDS |
| CTGCTTCTGGTTGATCTACC+AGG | 0.434517 | 1.1:-75991072 | MS.gene93418:CDS |
| GATTACTGCTTAAATCCAAT+TGG | 0.439581 | 1.1:+75990205 | None:intergenic |
| AACTGATCTTGACACATTGC+CGG | 0.441015 | 1.1:-75989587 | MS.gene93418:CDS |
| AAAGGACCTTCTTCCGGGTA+AGG | 0.449636 | 1.1:-75990955 | MS.gene93418:CDS |
| TAAGCACGGAGCCGAAGAGC+AGG | 0.458636 | 1.1:-75990745 | MS.gene93418:CDS |
| AGTAGGAATCAAAGATCTAA+AGG | 0.460334 | 1.1:-75991140 | MS.gene93418:CDS |
| ATTACCAGGTAGGTTTCCTT+CGG | 0.462002 | 1.1:+75989993 | None:intergenic |
| AGTGAAGAGGTGGAAGATGA+AGG | 0.462568 | 1.1:-75990705 | MS.gene93418:CDS |
| CAACAACAAGAAGGAGAAGA+AGG | 0.465449 | 1.1:-75990843 | MS.gene93418:CDS |
| GATGACACGTCAAGTTCATC+TGG | 0.468805 | 1.1:-75990135 | MS.gene93418:CDS |
| AACAAACCCCGATAGCCAAT+TGG | 0.479844 | 1.1:-75990220 | MS.gene93418:CDS |
| TCAAAGATCTAAAGGATTCA+AGG | 0.480436 | 1.1:-75991132 | MS.gene93418:CDS |
| ATTTCTTCAAATGCAACTGC+TGG | 0.480974 | 1.1:-75990285 | MS.gene93418:CDS |
| TGATCCGTCTCAGTTATAAT+CGG | 0.485326 | 1.1:+75990996 | None:intergenic |
| GCTTAAATCCAATTGGCTAT+CGG | 0.489720 | 1.1:+75990212 | None:intergenic |
| GATTCTGAAACTTGGTAGAA+AGG | 0.489839 | 1.1:-75990973 | MS.gene93418:CDS |
| TTGCCGGATATTAGAAATGA+AGG | 0.493543 | 1.1:-75989571 | MS.gene93418:CDS |
| GTGAAGACAACGTGCATGAA+GGG | 0.494786 | 1.1:-75990794 | MS.gene93418:CDS |
| ACAAGGAGGATGAGGAAAAG+GGG | 0.499091 | 1.1:-75990557 | MS.gene93418:CDS |
| AGAAGAGGGTGATGATGAGA+CGG | 0.505523 | 1.1:-75990589 | MS.gene93418:CDS |
| GCAACTGCTGGTAAAGAGGC+TGG | 0.509590 | 1.1:-75990273 | MS.gene93418:CDS |
| TGATCGTGATGATGAGGTTG+TGG | 0.514590 | 1.1:-75990631 | MS.gene93418:CDS |
| AAATGCGAGCTCTGATACCA+CGG | 0.515293 | 1.1:-75989830 | MS.gene93418:CDS |
| TTTAGATTTGTCACTTTCCG+TGG | 0.518761 | 1.1:+75989813 | None:intergenic |
| AGCAGTAACTCGACAGGCAC+TGG | 0.519435 | 1.1:-75990159 | MS.gene93418:CDS |
| ACCACGACAATCATGAAGCT+CGG | 0.520214 | 1.1:-75990521 | MS.gene93418:CDS |
| CCACGACAATCATGAAGCTC+GGG | 0.521569 | 1.1:-75990520 | MS.gene93418:CDS |
| AGAGAAAGACAAAGAAGAAG+AGG | 0.523414 | 1.1:-75990604 | MS.gene93418:CDS |
| TGTCATGATTGTGCTTAACC+TGG | 0.523771 | 1.1:+75991054 | None:intergenic |
| TGACAGTTTGTCTAATGAAA+CGG | 0.525886 | 1.1:-75990247 | MS.gene93418:CDS |
| CCCGAGCTTCATGATTGTCG+TGG | 0.527799 | 1.1:+75990520 | None:intergenic |
| TGAAAGCAAAAGTGAAGAGG+TGG | 0.527884 | 1.1:-75990715 | MS.gene93418:CDS |
| AAATGCAACTGCTGGTAAAG+AGG | 0.532472 | 1.1:-75990277 | MS.gene93418:CDS |
| TACCGAGGATGAGATGTTCA+AGG | 0.534057 | 1.1:-75989743 | MS.gene93418:CDS |
| CTTCAACCTTACCCGGAAGA+AGG | 0.542817 | 1.1:+75990949 | None:intergenic |
| GAGAAAGACAAAGAAGAAGA+GGG | 0.543819 | 1.1:-75990603 | MS.gene93418:CDS |
| TTATCTGCTTCAACCTTACC+CGG | 0.547125 | 1.1:+75990942 | None:intergenic |
| CAAGGAGGATGAGGAAAAGG+GGG | 0.549163 | 1.1:-75990556 | MS.gene93418:CDS |
| TGAAAAGCACGAGGAAGAAG+AGG | 0.550031 | 1.1:-75990916 | MS.gene93418:CDS |
| GCACAATCATGACAAGAAGA+AGG | 0.554608 | 1.1:-75991045 | MS.gene93418:CDS |
| GAAGAGGTGGAAGATGAAGG+AGG | 0.566940 | 1.1:-75990702 | MS.gene93418:CDS |
| ACGGATCAGATTCTGAAACT+TGG | 0.567290 | 1.1:-75990981 | MS.gene93418:CDS |
| GAATGAAGACAAGGAGGATG+AGG | 0.567800 | 1.1:-75990565 | MS.gene93418:CDS |
| ACGGAAAGTGACAAATCTAA+AGG | 0.571831 | 1.1:-75989811 | MS.gene93418:CDS |
| ACCCTTGAACATCTCATCCT+CGG | 0.573116 | 1.1:+75989741 | None:intergenic |
| CAATTGAATCATTACCAGGT+AGG | 0.578816 | 1.1:+75989983 | None:intergenic |
| TTAAATCCAATTGGCTATCG+GGG | 0.580558 | 1.1:+75990214 | None:intergenic |
| ATCATGTGCCACAGCACTAG+AGG | 0.584624 | 1.1:+75990473 | None:intergenic |
| GCACGGAGCCGAAGAGCAGG+AGG | 0.585830 | 1.1:-75990742 | MS.gene93418:CDS |
| GGAGATATAAAACAAACAGA+TGG | 0.586089 | 1.1:-75990042 | MS.gene93418:CDS |
| GACGCAAGCAGTAACTCGAC+AGG | 0.588343 | 1.1:-75990165 | MS.gene93418:CDS |
| CACGATGAACAACAACAAGA+AGG | 0.590155 | 1.1:-75990852 | MS.gene93418:CDS |
| TCTGATTTGAAGGTTACAGA+AGG | 0.593375 | 1.1:-75990324 | MS.gene93418:CDS |
| AGCTCGGGAAGAACATTACA+AGG | 0.597038 | 1.1:-75990505 | MS.gene93418:CDS |
| AAATGAAAGCAAAAGTGAAG+AGG | 0.597712 | 1.1:-75990718 | MS.gene93418:CDS |
| AGTGAAGACAACGTGCATGA+AGG | 0.602024 | 1.1:-75990795 | MS.gene93418:CDS |
| ATTTCCGAAGGAAACCTACC+TGG | 0.602219 | 1.1:-75989997 | MS.gene93418:CDS |
| GAAACAATTGAATCATTACC+AGG | 0.603201 | 1.1:+75989979 | None:intergenic |
| ATCTAAAATTTCAGTGACAG+AGG | 0.616818 | 1.1:+75989678 | None:intergenic |
| TGTTAAACCTGAAAATGGAG+TGG | 0.618379 | 1.1:-75989956 | MS.gene93418:CDS |
| ACCGAGGATGAGATGTTCAA+GGG | 0.630659 | 1.1:-75989742 | MS.gene93418:CDS |
| GATGAAGAAGAAAATAAGCA+CGG | 0.630678 | 1.1:-75990759 | MS.gene93418:CDS |
| TGATGATGCCTCTAGTGCTG+TGG | 0.631001 | 1.1:-75990481 | MS.gene93418:CDS |
| CATACATGAGGATATGGCAA+AGG | 0.631044 | 1.1:-75989614 | MS.gene93418:CDS |
| ACAGAAGGTGAATTGACAGA+TGG | 0.633370 | 1.1:-75990309 | MS.gene93418:CDS |
| TCAGAATACTGACGCTACCG+AGG | 0.636306 | 1.1:-75989758 | MS.gene93418:CDS |
| AAGGAGGATGAGGAAAAGGG+GGG | 0.640271 | 1.1:-75990555 | MS.gene93418:CDS |
| ACTCAAACAAGTGAAACCGA+TGG | 0.642931 | 1.1:-75989715 | MS.gene93418:CDS |
| AGTGGCAGTAAAATTTCCGA+AGG | 0.656357 | 1.1:-75990009 | MS.gene93418:CDS |
| AGTGCATTAGAGAAGACGAT+CGG | 0.671772 | 1.1:-75989901 | MS.gene93418:CDS |
| AAATGATCATGAGAAATCAG+AGG | 0.674111 | 1.1:-75990661 | MS.gene93418:CDS |
| GGATACTCAAAACATACATG+AGG | 0.674707 | 1.1:-75989626 | MS.gene93418:CDS |
| AGATAATGATCGTGATGATG+AGG | 0.692551 | 1.1:-75990637 | MS.gene93418:CDS |
| ACTGCCGATTATAACTGAGA+CGG | 0.699045 | 1.1:-75991000 | MS.gene93418:CDS |
| TGAGACGGAGAATGAAGACA+AGG | 0.700440 | 1.1:-75990574 | MS.gene93418:CDS |
| AGATAAGAATGAAAAGCACG+AGG | 0.713312 | 1.1:-75990925 | MS.gene93418:CDS |
| GACGGAGAATGAAGACAAGG+AGG | 0.719650 | 1.1:-75990571 | MS.gene93418:CDS |
| ACAGATGGATTAGAGCAGAG+TGG | 0.720182 | 1.1:-75990027 | MS.gene93418:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAGTTAATACAACAATTAC+TGG | - | chr1.1:75990638-75990657 | MS.gene93418:CDS | 25.0% |
| ! | TTTCCTTCATTTCTAATATC+CGG | + | chr1.1:75991136-75991155 | None:intergenic | 25.0% |
| !! | TCAAAATGATTCTGATTTGA+AGG | - | chr1.1:75990367-75990386 | MS.gene93418:CDS | 25.0% |
| AAATGAAAGCAAAAGTGAAG+AGG | - | chr1.1:75989983-75990002 | MS.gene93418:CDS | 30.0% | |
| AAATGATCATGAGAAATCAG+AGG | - | chr1.1:75990040-75990059 | MS.gene93418:CDS | 30.0% | |
| AGTAGGAATCAAAGATCTAA+AGG | - | chr1.1:75989561-75989580 | MS.gene93418:CDS | 30.0% | |
| ATCATTCTGATGAAAGTAAC+AGG | - | chr1.1:75990342-75990361 | MS.gene93418:CDS | 30.0% | |
| GAAACAATTGAATCATTACC+AGG | + | chr1.1:75990725-75990744 | None:intergenic | 30.0% | |
| GATGAAGAAGAAAATAAGCA+CGG | - | chr1.1:75989942-75989961 | MS.gene93418:CDS | 30.0% | |
| GATTACTGCTTAAATCCAAT+TGG | + | chr1.1:75990499-75990518 | None:intergenic | 30.0% | |
| GGAGATATAAAACAAACAGA+TGG | - | chr1.1:75990659-75990678 | MS.gene93418:CDS | 30.0% | |
| GTTTCTGTTAAACCTGAAAA+TGG | - | chr1.1:75990740-75990759 | MS.gene93418:CDS | 30.0% | |
| TAAAAAGGAATCCAAGTAGT+AGG | - | chr1.1:75989544-75989563 | MS.gene93418:CDS | 30.0% | |
| TCAAAACATACATGAGGATA+TGG | - | chr1.1:75991081-75991100 | MS.gene93418:CDS | 30.0% | |
| TCAAAGATCTAAAGGATTCA+AGG | - | chr1.1:75989569-75989588 | MS.gene93418:CDS | 30.0% | |
| TGACAGTTTGTCTAATGAAA+CGG | - | chr1.1:75990454-75990473 | MS.gene93418:CDS | 30.0% | |
| ! | ATCTAAAATTTCAGTGACAG+AGG | + | chr1.1:75991026-75991045 | None:intergenic | 30.0% |
| ! | TAGCACTGAAACAAGTAATT+TGG | - | chr1.1:75990262-75990281 | MS.gene93418:CDS | 30.0% |
| ACGGAAAGTGACAAATCTAA+AGG | - | chr1.1:75990890-75990909 | MS.gene93418:CDS | 35.0% | |
| AGAGAAAGACAAAGAAGAAG+AGG | - | chr1.1:75990097-75990116 | MS.gene93418:CDS | 35.0% | |
| ATTTCTTCAAATGCAACTGC+TGG | - | chr1.1:75990416-75990435 | MS.gene93418:CDS | 35.0% | |
| CAATTGAATCATTACCAGGT+AGG | + | chr1.1:75990721-75990740 | None:intergenic | 35.0% | |
| CGCATTTCTATCCAAATTAC+TGG | + | chr1.1:75990858-75990877 | None:intergenic | 35.0% | |
| CTGAAAAATCTTCGACTCTT+GGG | - | chr1.1:75990774-75990793 | MS.gene93418:CDS | 35.0% | |
| CTTAAATCCAATTGGCTATC+GGG | + | chr1.1:75990491-75990510 | None:intergenic | 35.0% | |
| GAGAAAGACAAAGAAGAAGA+GGG | - | chr1.1:75990098-75990117 | MS.gene93418:CDS | 35.0% | |
| GATCTTTGATTCCTACTACT+TGG | + | chr1.1:75989558-75989577 | None:intergenic | 35.0% | |
| GCTTAAATCCAATTGGCTAT+CGG | + | chr1.1:75990492-75990511 | None:intergenic | 35.0% | |
| GGATACTCAAAACATACATG+AGG | - | chr1.1:75991075-75991094 | MS.gene93418:CDS | 35.0% | |
| TGAAAAATCTTCGACTCTTG+GGG | - | chr1.1:75990775-75990794 | MS.gene93418:CDS | 35.0% | |
| TGATCCGTCTCAGTTATAAT+CGG | + | chr1.1:75989708-75989727 | None:intergenic | 35.0% | |
| TGTTAAACCTGAAAATGGAG+TGG | - | chr1.1:75990745-75990764 | MS.gene93418:CDS | 35.0% | |
| TGTTAACGTGTCCAGTAATT+TGG | - | chr1.1:75990844-75990863 | MS.gene93418:CDS | 35.0% | |
| TTAAATCCAATTGGCTATCG+GGG | + | chr1.1:75990490-75990509 | None:intergenic | 35.0% | |
| TTGCCGGATATTAGAAATGA+AGG | - | chr1.1:75991130-75991149 | MS.gene93418:CDS | 35.0% | |
| ! | AGATAAGAATGAAAAGCACG+AGG | - | chr1.1:75989776-75989795 | MS.gene93418:CDS | 35.0% |
| ! | AGATAATGATCGTGATGATG+AGG | - | chr1.1:75990064-75990083 | MS.gene93418:CDS | 35.0% |
| ! | ATTTTGAGCATGATCAGACT+CGG | + | chr1.1:75990615-75990634 | None:intergenic | 35.0% |
| ! | TCTGATTTGAAGGTTACAGA+AGG | - | chr1.1:75990377-75990396 | MS.gene93418:CDS | 35.0% |
| !! | ACACGACACTATTGATTCTT+CGG | - | chr1.1:75991054-75991073 | MS.gene93418:CDS | 35.0% |
| !! | GATTCTGAAACTTGGTAGAA+AGG | - | chr1.1:75989728-75989747 | MS.gene93418:CDS | 35.0% |
| !! | TTTAGATTTGTCACTTTCCG+TGG | + | chr1.1:75990891-75990910 | None:intergenic | 35.0% |
| AAATGCAACTGCTGGTAAAG+AGG | - | chr1.1:75990424-75990443 | MS.gene93418:CDS | 40.0% | |
| AACTGATCTTGACACATTGC+CGG | - | chr1.1:75991114-75991133 | MS.gene93418:CDS | 40.0% | |
| ACAGAAGGTGAATTGACAGA+TGG | - | chr1.1:75990392-75990411 | MS.gene93418:CDS | 40.0% | |
| ACTCAAACAAGTGAAACCGA+TGG | - | chr1.1:75990986-75991005 | MS.gene93418:CDS | 40.0% | |
| AGTGCATTAGAGAAGACGAT+CGG | - | chr1.1:75990800-75990819 | MS.gene93418:CDS | 40.0% | |
| AGTGGCAGTAAAATTTCCGA+AGG | - | chr1.1:75990692-75990711 | MS.gene93418:CDS | 40.0% | |
| ATTACCAGGTAGGTTTCCTT+CGG | + | chr1.1:75990711-75990730 | None:intergenic | 40.0% | |
| CAACAACAAGAAGGAGAAGA+AGG | - | chr1.1:75989858-75989877 | MS.gene93418:CDS | 40.0% | |
| CACGATGAACAACAACAAGA+AGG | - | chr1.1:75989849-75989868 | MS.gene93418:CDS | 40.0% | |
| CATACATGAGGATATGGCAA+AGG | - | chr1.1:75991087-75991106 | MS.gene93418:CDS | 40.0% | |
| CCTGAAAAATCTTCGACTCT+TGG | - | chr1.1:75990773-75990792 | MS.gene93418:CDS | 40.0% | |
| CTTCAAGCTATTCTGTTGCT+TGG | - | chr1.1:75989603-75989622 | MS.gene93418:CDS | 40.0% | |
| GCACAATCATGACAAGAAGA+AGG | - | chr1.1:75989656-75989675 | MS.gene93418:CDS | 40.0% | |
| TGAAAGCAAAAGTGAAGAGG+TGG | - | chr1.1:75989986-75990005 | MS.gene93418:CDS | 40.0% | |
| TGTCATGATTGTGCTTAACC+TGG | + | chr1.1:75989650-75989669 | None:intergenic | 40.0% | |
| TTATCTGCTTCAACCTTACC+CGG | + | chr1.1:75989762-75989781 | None:intergenic | 40.0% | |
| ! | ACTGCCGATTATAACTGAGA+CGG | - | chr1.1:75989701-75989720 | MS.gene93418:CDS | 40.0% |
| ! | CCAAGAGTCGAAGATTTTTC+AGG | + | chr1.1:75990776-75990795 | None:intergenic | 40.0% |
| ! | GGATGTGTCTGATTTTCCAT+CGG | + | chr1.1:75991005-75991024 | None:intergenic | 40.0% |
| ! | TGATGAGTTGTCTCGTTTTC+AGG | + | chr1.1:75990326-75990345 | None:intergenic | 40.0% |
| ! | TTCATTTTCCTCCTGCTCTT+CGG | + | chr1.1:75989970-75989989 | None:intergenic | 40.0% |
| !! | ACGGATCAGATTCTGAAACT+TGG | - | chr1.1:75989720-75989739 | MS.gene93418:CDS | 40.0% |
| !!! | TGTCTCGTTTTCAGGTTTTC+TGG | + | chr1.1:75990318-75990337 | None:intergenic | 40.0% |
| AACAAACCCCGATAGCCAAT+TGG | - | chr1.1:75990481-75990500 | MS.gene93418:CDS | 45.0% | |
| ACAAGGAGGATGAGGAAAAG+GGG | - | chr1.1:75990144-75990163 | MS.gene93418:CDS | 45.0% | |
| ACCACGACAATCATGAAGCT+CGG | - | chr1.1:75990180-75990199 | MS.gene93418:CDS | 45.0% | |
| ACCCTTGAACATCTCATCCT+CGG | + | chr1.1:75990963-75990982 | None:intergenic | 45.0% | |
| ACCGAGGATGAGATGTTCAA+GGG | - | chr1.1:75990959-75990978 | MS.gene93418:CDS | 45.0% | |
| AGACAAGGAGGATGAGGAAA+AGG | - | chr1.1:75990142-75990161 | MS.gene93418:CDS | 45.0% | |
| AGCTCGGGAAGAACATTACA+AGG | - | chr1.1:75990196-75990215 | MS.gene93418:CDS | 45.0% | |
| AGTGAAGACAACGTGCATGA+AGG | - | chr1.1:75989906-75989925 | MS.gene93418:CDS | 45.0% | |
| AGTGAAGAGGTGGAAGATGA+AGG | - | chr1.1:75989996-75990015 | MS.gene93418:CDS | 45.0% | |
| ATTTCCGAAGGAAACCTACC+TGG | - | chr1.1:75990704-75990723 | MS.gene93418:CDS | 45.0% | |
| GAATGAAGACAAGGAGGATG+AGG | - | chr1.1:75990136-75990155 | MS.gene93418:CDS | 45.0% | |
| GACAAGGAGGATGAGGAAAA+GGG | - | chr1.1:75990143-75990162 | MS.gene93418:CDS | 45.0% | |
| GATGACACGTCAAGTTCATC+TGG | - | chr1.1:75990566-75990585 | MS.gene93418:CDS | 45.0% | |
| GTGAAGACAACGTGCATGAA+GGG | - | chr1.1:75989907-75989926 | MS.gene93418:CDS | 45.0% | |
| TACCGAGGATGAGATGTTCA+AGG | - | chr1.1:75990958-75990977 | MS.gene93418:CDS | 45.0% | |
| TGAGACGGAGAATGAAGACA+AGG | - | chr1.1:75990127-75990146 | MS.gene93418:CDS | 45.0% | |
| TGGTAGAAAGGACCTTCTTC+CGG | - | chr1.1:75989740-75989759 | MS.gene93418:CDS | 45.0% | |
| ! | AAATGCGAGCTCTGATACCA+CGG | - | chr1.1:75990871-75990890 | MS.gene93418:CDS | 45.0% |
| ! | AGAAGAGGGTGATGATGAGA+CGG | - | chr1.1:75990112-75990131 | MS.gene93418:CDS | 45.0% |
| ! | TGAAAAGCACGAGGAAGAAG+AGG | - | chr1.1:75989785-75989804 | MS.gene93418:CDS | 45.0% |
| ! | TGATCGTGATGATGAGGTTG+TGG | - | chr1.1:75990070-75990089 | MS.gene93418:CDS | 45.0% |
| !! | ACAGATGGATTAGAGCAGAG+TGG | - | chr1.1:75990674-75990693 | MS.gene93418:CDS | 45.0% |
| !! | TTGGCTATCGGGGTTTGTTT+TGG | + | chr1.1:75990480-75990499 | None:intergenic | 45.0% |
| AAAGGACCTTCTTCCGGGTA+AGG | - | chr1.1:75989746-75989765 | MS.gene93418:CDS | 50.0% | |
| AAGGAGGATGAGGAAAAGGG+GGG | - | chr1.1:75990146-75990165 | MS.gene93418:CDS | 50.0% | |
| ATCATGTGCCACAGCACTAG+AGG | + | chr1.1:75990231-75990250 | None:intergenic | 50.0% | |
| CAAGGAGGATGAGGAAAAGG+GGG | - | chr1.1:75990145-75990164 | MS.gene93418:CDS | 50.0% | |
| CCACGACAATCATGAAGCTC+GGG | - | chr1.1:75990181-75990200 | MS.gene93418:CDS | 50.0% | |
| CTTCAACCTTACCCGGAAGA+AGG | + | chr1.1:75989755-75989774 | None:intergenic | 50.0% | |
| GAAGAGGTGGAAGATGAAGG+AGG | - | chr1.1:75989999-75990018 | MS.gene93418:CDS | 50.0% | |
| GACGGAGAATGAAGACAAGG+AGG | - | chr1.1:75990130-75990149 | MS.gene93418:CDS | 50.0% | |
| GGTAGAAAGGACCTTCTTCC+GGG | - | chr1.1:75989741-75989760 | MS.gene93418:CDS | 50.0% | |
| TCAGAATACTGACGCTACCG+AGG | - | chr1.1:75990943-75990962 | MS.gene93418:CDS | 50.0% | |
| ! | CTGCTTCTGGTTGATCTACC+AGG | - | chr1.1:75989629-75989648 | MS.gene93418:CDS | 50.0% |
| ! | TGATGATGCCTCTAGTGCTG+TGG | - | chr1.1:75990220-75990239 | MS.gene93418:CDS | 50.0% |
| !! | TGTTGCTTGGTGTCTGCTTC+TGG | - | chr1.1:75989616-75989635 | MS.gene93418:CDS | 50.0% |
| AGCAGTAACTCGACAGGCAC+TGG | - | chr1.1:75990542-75990561 | MS.gene93418:CDS | 55.0% | |
| CCCGAGCTTCATGATTGTCG+TGG | + | chr1.1:75990184-75990203 | None:intergenic | 55.0% | |
| GACGCAAGCAGTAACTCGAC+AGG | - | chr1.1:75990536-75990555 | MS.gene93418:CDS | 55.0% | |
| GCAACTGCTGGTAAAGAGGC+TGG | - | chr1.1:75990428-75990447 | MS.gene93418:CDS | 55.0% | |
| ! | GGAGCTGCCACTCCATTTTC+AGG | + | chr1.1:75990755-75990774 | None:intergenic | 55.0% |
| TAAGCACGGAGCCGAAGAGC+AGG | - | chr1.1:75989956-75989975 | MS.gene93418:CDS | 60.0% | |
| GCACGGAGCCGAAGAGCAGG+AGG | - | chr1.1:75989959-75989978 | MS.gene93418:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 75989540 | 75991183 | 75989540 | ID=MS.gene93418 |
| chr1.1 | mRNA | 75989540 | 75991183 | 75989540 | ID=MS.gene93418.t1;Parent=MS.gene93418 |
| chr1.1 | exon | 75989540 | 75991183 | 75989540 | ID=MS.gene93418.t1.exon1;Parent=MS.gene93418.t1 |
| chr1.1 | CDS | 75989540 | 75991183 | 75989540 | ID=cds.MS.gene93418.t1;Parent=MS.gene93418.t1 |
| Gene Sequence |
| Protein sequence |
