AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001167.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001167.01.T01 MTR_1g032430 84.906 212 9 1 1 212 1 189 1.39e-127 359
MsG0180001167.01.T01 MTR_1g032430 84.906 212 9 1 1 212 1 189 5.11e-126 358
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001167.01.T01 AT1G24793 63.006 173 64 0 41 213 35 207 5.06e-74 224
MsG0180001167.01.T01 AT1G25210 63.006 173 64 0 41 213 35 207 5.06e-74 224
MsG0180001167.01.T01 AT1G24793 63.006 173 64 0 41 213 35 207 1.12e-72 223
MsG0180001167.01.T01 AT1G25054 63.006 173 64 0 41 213 35 207 1.12e-72 223
MsG0180001167.01.T01 AT1G25145 63.006 173 64 0 41 213 35 207 1.12e-72 223
MsG0180001167.01.T01 AT1G25210 63.006 173 64 0 41 213 35 207 1.12e-72 223

Find 66 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGATTCAGATTGAAAATT+TGG 0.152736 1:+16769807 MsG0180001167.01.T01:CDS
CTTTCTGCGTTGGAAGGTTC+TGG 0.164574 1:+16769772 MsG0180001167.01.T01:CDS
AAGGGAATCTCATTGCATTC+TGG 0.296825 1:+16769586 MsG0180001167.01.T01:CDS
AAGGAAGGTGGTTTGAGTTT+AGG 0.298029 1:+16769650 MsG0180001167.01.T01:CDS
GTTTGAGTTTAGGAACAATT+TGG 0.329929 1:+16769660 MsG0180001167.01.T01:CDS
TATAAGAGAGAGTGAGTTAC+GGG 0.346205 1:-16769472 None:intergenic
TTTCTGCGTTGGAAGGTTCT+GGG 0.353515 1:+16769773 MsG0180001167.01.T01:CDS
GAGATAGCAACTGATGTTGA+TGG 0.375553 1:+16771407 MsG0180001167.01.T01:CDS
GCGCAGATTCCTATATTTGA+TGG 0.390435 1:+16771341 MsG0180001167.01.T01:intron
AGATTGTGTCCTGGATTTGC+AGG 0.390889 1:+16769625 MsG0180001167.01.T01:CDS
GAAGTGGTTGGAGTAACTTA+TGG 0.399861 1:+16771515 MsG0180001167.01.T01:CDS
AAATTTGAAATGAAGCAAAC+AGG 0.416482 1:-16769496 None:intergenic
GTAAAGATGGTGTGAGGATT+AGG 0.425255 1:+16769734 MsG0180001167.01.T01:CDS
CATCATCTCTTTGTGCAGAC+GGG 0.429286 1:+16769526 MsG0180001167.01.T01:CDS
TAGGGAATGGGTGGCAGCCT+TGG 0.431192 1:+16771370 MsG0180001167.01.T01:CDS
TGATGGGTCGGCTAGGGAAT+GGG 0.431195 1:+16771358 MsG0180001167.01.T01:CDS
GTTCTGGGGTTGATAATTGT+AGG 0.431213 1:+16769788 MsG0180001167.01.T01:CDS
AGGGAATCTCATTGCATTCT+GGG 0.433544 1:+16769587 MsG0180001167.01.T01:CDS
TGTACTACTTTGTGTAAAGA+TGG 0.443962 1:+16769721 MsG0180001167.01.T01:CDS
TGAATGAACCGGTACATGTT+TGG 0.458151 1:+16771459 MsG0180001167.01.T01:CDS
ATTGAAAATTTGGATGATCA+AGG 0.461008 1:+16769817 MsG0180001167.01.T01:CDS
CTAGCCGACCCATCAAATAT+AGG 0.461299 1:-16771350 None:intergenic
TTGATGGGTCGGCTAGGGAA+TGG 0.472944 1:+16771357 MsG0180001167.01.T01:CDS
CATTTACTTTCTGCGTTGGA+AGG 0.492345 1:+16769766 MsG0180001167.01.T01:CDS
CTATATTTGATGGGTCGGCT+AGG 0.502389 1:+16771351 MsG0180001167.01.T01:CDS
CTTCCTTGACCTGCAAATCC+AGG 0.503251 1:-16769634 None:intergenic
AGCTCCACATGTGAATGAAC+CGG 0.504944 1:+16771448 MsG0180001167.01.T01:CDS
TGCAGCATTTCCTTCAGAAG+TGG 0.509016 1:+16771499 MsG0180001167.01.T01:CDS
TCATCATCTCTTTGTGCAGA+CGG 0.509214 1:+16769525 MsG0180001167.01.T01:CDS
TATATTTGATGGGTCGGCTA+GGG 0.519772 1:+16771352 MsG0180001167.01.T01:CDS
CGCAGATTCCTATATTTGAT+GGG 0.523997 1:+16771342 MsG0180001167.01.T01:intron
GTATAAGAGAGAGTGAGTTA+CGG 0.524369 1:-16769473 None:intergenic
GTGGCAGCCTTGGAAGAAGT+TGG 0.528078 1:+16771380 MsG0180001167.01.T01:CDS
AAGCTTCAACAAACTCTAAG+AGG 0.529912 1:+16769550 MsG0180001167.01.T01:CDS
GCATTTCCTTCAGAAGTGGT+TGG 0.531128 1:+16771503 MsG0180001167.01.T01:CDS
GCATAAACTTTCCACAGGTA+AGG 0.533834 1:+16771537 MsG0180001167.01.T01:CDS
ACAAACTCTAAGAGGAATTG+TGG 0.537569 1:+16769558 MsG0180001167.01.T01:CDS
AAGAGGAATTGTGGAGCGGA+AGG 0.538994 1:+16769567 MsG0180001167.01.T01:CDS
GATTCCTATATTTGATGGGT+CGG 0.549220 1:+16771346 MsG0180001167.01.T01:intron
CTCTAAACCAACTTCTTCCA+AGG 0.554837 1:-16771387 None:intergenic
TGAGCAAATTCAACAGAAGC+AGG 0.556051 1:-16769685 None:intergenic
TTCTGCGTTGGAAGGTTCTG+GGG 0.564192 1:+16769774 MsG0180001167.01.T01:CDS
GGAGCATTTACTTTCTGCGT+TGG 0.564598 1:+16769762 MsG0180001167.01.T01:CDS
TGTCCTGGATTTGCAGGTCA+AGG 0.566608 1:+16769631 MsG0180001167.01.T01:CDS
TCATTCCTCCAAACATGTAC+CGG 0.567307 1:-16771467 None:intergenic
GATCATCAAAACTCGTCACA+TGG 0.570839 1:+16769446 MsG0180001167.01.T01:CDS
CTGGATTTGCAGGTCAAGGA+AGG 0.576111 1:+16769635 MsG0180001167.01.T01:CDS
AACTTTCCACAGGTAAGGAT+AGG 0.576646 1:+16771542 MsG0180001167.01.T01:CDS
GTTACTCCAACCACTTCTGA+AGG 0.580389 1:-16771509 None:intergenic
TTATGGCATAAACTTTCCAC+AGG 0.581378 1:+16771532 MsG0180001167.01.T01:CDS
GATTTGCAGGTCAAGGAAGG+TGG 0.585501 1:+16769638 MsG0180001167.01.T01:CDS
ATCATCTCTTTGTGCAGACG+GGG 0.586150 1:+16769527 MsG0180001167.01.T01:CDS
ATGAACCGGTACATGTTTGG+AGG 0.594386 1:+16771462 MsG0180001167.01.T01:CDS
AATTTGAAATGAAGCAAACA+GGG 0.602335 1:-16769495 None:intergenic
TCGACGGTGAGATTGTGTCC+TGG 0.609644 1:+16769616 MsG0180001167.01.T01:CDS
AATCTCATTGCATTCTGGGA+AGG 0.617901 1:+16769591 MsG0180001167.01.T01:CDS
TATCAACCTATCCTTACCTG+TGG 0.621241 1:-16771548 None:intergenic
AGAGGAATTGTGGAGCGGAA+GGG 0.632291 1:+16769568 MsG0180001167.01.T01:CDS
CTCTAAGAGGAATTGTGGAG+CGG 0.643430 1:+16769563 MsG0180001167.01.T01:CDS
GCATTCTGGGAAGGTATCGA+CGG 0.651052 1:+16769600 MsG0180001167.01.T01:CDS
TGGGTCGGCTAGGGAATGGG+TGG 0.651920 1:+16771361 MsG0180001167.01.T01:CDS
TGGTGTGAGGATTAGGACTG+TGG 0.685106 1:+16769741 MsG0180001167.01.T01:CDS
CTTTGTGTAAAGATGGTGTG+AGG 0.705362 1:+16769728 MsG0180001167.01.T01:CDS
TGATCAAGGTGATGATGCCG+AGG 0.707861 1:+16769831 MsG0180001167.01.T01:CDS
TGTACCGGTTCATTCACATG+TGG 0.732995 1:-16771452 None:intergenic
TTACACAAAGTAGTACAAAG+CGG 0.755266 1:-16769715 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAATACAAACT+TGG + Chr1:16770316-16770335 MsG0180001167.01.T01:intron 10.0%
!! AATAAGTTAAAAACAACTAA+CGG + Chr1:16771007-16771026 MsG0180001167.01.T01:intron 15.0%
!! AGAATGTAATGTATTAATAT+GGG + Chr1:16769938-16769957 MsG0180001167.01.T01:intron 15.0%
!! TAGAATGTAATGTATTAATA+TGG + Chr1:16769937-16769956 MsG0180001167.01.T01:intron 15.0%
!!! AGTTTATTTAGAACTTATTT+GGG - Chr1:16771092-16771111 None:intergenic 15.0%
!! AACTTATTTCAACAACTTAT+TGG - Chr1:16770995-16771014 None:intergenic 20.0%
!! ACAGCAAGAAAATTTAATTA+CGG - Chr1:16771271-16771290 None:intergenic 20.0%
!! ATAATCTTAGAAATTGTTGA+CGG - Chr1:16770624-16770643 None:intergenic 20.0%
!! CTTAAAAATAGTTTACTCAA+AGG - Chr1:16770961-16770980 None:intergenic 20.0%
!! TATTTCAAGAACTAAATGTA+AGG - Chr1:16771295-16771314 None:intergenic 20.0%
!! TGGGTTTATTAATTATATCA+TGG - Chr1:16771073-16771092 None:intergenic 20.0%
!! TTAAATAAGTCGATATAAAC+AGG - Chr1:16769964-16769983 None:intergenic 20.0%
!! TTGAATAAGTGTTTATAAGT+GGG + Chr1:16770375-16770394 MsG0180001167.01.T01:intron 20.0%
!!! GAGTTTATTTAGAACTTATT+TGG - Chr1:16771093-16771112 None:intergenic 20.0%
! ATAAACACATAAACACTTGT+AGG + Chr1:16770646-16770665 MsG0180001167.01.T01:intron 25.0%
! ATTGAAAATTTGGATGATCA+AGG + Chr1:16769817-16769836 MsG0180001167.01.T01:CDS 25.0%
! CTTGAATAAGTGTTTATAAG+TGG + Chr1:16770374-16770393 MsG0180001167.01.T01:intron 25.0%
! CTTTATACATAGCGAATATA+AGG + Chr1:16770185-16770204 MsG0180001167.01.T01:intron 25.0%
! GAGTTTATAATATGTGACAT+AGG - Chr1:16770707-16770726 None:intergenic 25.0%
! GTATATGTGAAAACTGTAAA+AGG - Chr1:16770729-16770748 None:intergenic 25.0%
! TAATACATTACATTCTACTC+AGG - Chr1:16769934-16769953 None:intergenic 25.0%
! TAATCTTAGAAATTGTTGAC+GGG - Chr1:16770623-16770642 None:intergenic 25.0%
! TAGGATTCAGATTGAAAATT+TGG + Chr1:16769807-16769826 MsG0180001167.01.T01:CDS 25.0%
! TGAATAAGTGTTTATAAGTG+GGG + Chr1:16770376-16770395 MsG0180001167.01.T01:intron 25.0%
! TTTACCACAAATCTCTAATA+AGG + Chr1:16771154-16771173 MsG0180001167.01.T01:intron 25.0%
! TTTGGTCCAAATATTATAAG+AGG + Chr1:16770933-16770952 MsG0180001167.01.T01:intron 25.0%
!! AAATCAAGATGTGCATATTT+TGG - Chr1:16770904-16770923 None:intergenic 25.0%
!! AAATTTGAAATGAAGCAAAC+AGG - Chr1:16769499-16769518 None:intergenic 25.0%
!! AATTTGAAATGAAGCAAACA+GGG - Chr1:16769498-16769517 None:intergenic 25.0%
!! CTTTTTCTCGTATATTATGA+AGG - Chr1:16771125-16771144 None:intergenic 25.0%
!! TTGTTTTCATATAGAGCTTA+TGG - Chr1:16770150-16770169 None:intergenic 25.0%
!!! CTGTTTTCAAGCTTATTTAA+TGG + Chr1:16770058-16770077 MsG0180001167.01.T01:intron 25.0%
!!! GCATATTTTGGATATTTTGT+TGG - Chr1:16770892-16770911 None:intergenic 25.0%
!!! TAATGTCTACTTTTCTAAGT+AGG - Chr1:16770754-16770773 None:intergenic 25.0%
!!! TTAGTTTTTATGTTCAGAGT+GGG - Chr1:16770275-16770294 None:intergenic 25.0%
AGACTGTTATAGATTGCTTA+CGG + Chr1:16770011-16770030 MsG0180001167.01.T01:intron 30.0%
AGATTTGTGGTAAACTACTA+AGG - Chr1:16771148-16771167 None:intergenic 30.0%
ATAGAGCTTATGGAAATAAG+TGG - Chr1:16770140-16770159 None:intergenic 30.0%
CATTTAACAACTTCTTACCT+CGG - Chr1:16769851-16769870 None:intergenic 30.0%
CCTGAATAAGTGTTTATAGT+GGG + Chr1:16770552-16770571 MsG0180001167.01.T01:intron 30.0%
CTATCCTTATTAGAGATTTG+TGG - Chr1:16771161-16771180 None:intergenic 30.0%
CTGAATAAGTGTTTATAGTG+GGG + Chr1:16770553-16770572 MsG0180001167.01.T01:intron 30.0%
GAATAAGTGTTTATAAGTGG+GGG + Chr1:16770377-16770396 MsG0180001167.01.T01:intron 30.0%
TGAATAAGTGTTTATAGTGG+GGG + Chr1:16770554-16770573 MsG0180001167.01.T01:intron 30.0%
TGTACTACTTTGTGTAAAGA+TGG + Chr1:16769721-16769740 MsG0180001167.01.T01:CDS 30.0%
TTACACAAAGTAGTACAAAG+CGG - Chr1:16769718-16769737 None:intergenic 30.0%
! AAGGTGCCTCTTATAATATT+TGG - Chr1:16770942-16770961 None:intergenic 30.0%
! AGATAGCATTTTCAATTTCG+TGG + Chr1:16771233-16771252 MsG0180001167.01.T01:intron 30.0%
! GTTTGAGTTTAGGAACAATT+TGG + Chr1:16769660-16769679 MsG0180001167.01.T01:CDS 30.0%
! TAGTTTTTATGTTCAGAGTG+GGG - Chr1:16770274-16770293 None:intergenic 30.0%
!! TCGTATATTATGAAGGCATT+TGG - Chr1:16771118-16771137 None:intergenic 30.0%
!!! GTTAGTTTTTATGTTCAGAG+TGG - Chr1:16770276-16770295 None:intergenic 30.0%
AAGCTTCAACAAACTCTAAG+AGG + Chr1:16769550-16769569 MsG0180001167.01.T01:CDS 35.0%
ACTTTCATATTCACTCTCCT+AGG + Chr1:16771191-16771210 MsG0180001167.01.T01:intron 35.0%
CACATAAACACTTGTAGGAT+AGG + Chr1:16770651-16770670 MsG0180001167.01.T01:intron 35.0%
CCCACTATAAACACTTATTC+AGG - Chr1:16770555-16770574 None:intergenic 35.0%
CGCAGATTCCTATATTTGAT+GGG + Chr1:16771342-16771361 MsG0180001167.01.T01:intron 35.0%
GCCTGAATAAGTGTTTATAG+TGG + Chr1:16770551-16770570 MsG0180001167.01.T01:intron 35.0%
GTATAAGAGAGAGTGAGTTA+CGG - Chr1:16769476-16769495 None:intergenic 35.0%
TAACACAACCTCTCAAAACT+GGG - Chr1:16770412-16770431 None:intergenic 35.0%
TATAAGAGAGAGTGAGTTAC+GGG - Chr1:16769475-16769494 None:intergenic 35.0%
TATGTGACATAGGTCATGTT+TGG - Chr1:16770697-16770716 None:intergenic 35.0%
TGATACCACCTTAGAAATTG+TGG - Chr1:16770442-16770461 None:intergenic 35.0%
TTAAAGAGTCCCACATTGAT+TGG + Chr1:16770522-16770541 MsG0180001167.01.T01:intron 35.0%
TTATGGCATAAACTTTCCAC+AGG + Chr1:16771532-16771551 MsG0180001167.01.T01:CDS 35.0%
! ACAAACTCTAAGAGGAATTG+TGG + Chr1:16769558-16769577 MsG0180001167.01.T01:CDS 35.0%
! ATGACTTGATGATTCAAGTG+GGG + Chr1:16770208-16770227 MsG0180001167.01.T01:intron 35.0%
! GAATAAGTGTTTATAGTGGG+GGG + Chr1:16770555-16770574 MsG0180001167.01.T01:intron 35.0%
! GATGACTTGATGATTCAAGT+GGG + Chr1:16770207-16770226 MsG0180001167.01.T01:intron 35.0%
! GATTCCTATATTTGATGGGT+CGG + Chr1:16771346-16771365 MsG0180001167.01.T01:intron 35.0%
! TAAAGAGTCCCACATTGATT+GGG + Chr1:16770523-16770542 MsG0180001167.01.T01:intron 35.0%
! TAATGTGTCCATTTTGCGTA+TGG + Chr1:16770778-16770797 MsG0180001167.01.T01:intron 35.0%
! TCTTGATTTCTGCAAGGTTT+TGG + Chr1:16770915-16770934 MsG0180001167.01.T01:intron 35.0%
!! ATTTTGATGAAACAGAGCCT+AGG - Chr1:16771211-16771230 None:intergenic 35.0%
AACTTTCCACAGGTAAGGAT+AGG + Chr1:16771542-16771561 MsG0180001167.01.T01:CDS 40.0%
AAGGGAATCTCATTGCATTC+TGG + Chr1:16769586-16769605 MsG0180001167.01.T01:CDS 40.0%
AATCAAAGAGCGGAGTGATA+AGG + Chr1:16770232-16770251 MsG0180001167.01.T01:intron 40.0%
ACCACCTTAGAAATTGTGGA+CGG - Chr1:16770438-16770457 None:intergenic 40.0%
AGGGAATCTCATTGCATTCT+GGG + Chr1:16769587-16769606 MsG0180001167.01.T01:CDS 40.0%
CATTTACTTTCTGCGTTGGA+AGG + Chr1:16769766-16769785 MsG0180001167.01.T01:CDS 40.0%
CTAACACAACCTCTCAAAAC+TGG - Chr1:16770413-16770432 None:intergenic 40.0%
CTCTAAACCAACTTCTTCCA+AGG - Chr1:16771390-16771409 None:intergenic 40.0%
CTTACACAACCTCACAAAAC+CGG - Chr1:16770599-16770618 None:intergenic 40.0%
CTTTGTGTAAAGATGGTGTG+AGG + Chr1:16769728-16769747 MsG0180001167.01.T01:CDS 40.0%
GAAGTGGTTGGAGTAACTTA+TGG + Chr1:16771515-16771534 MsG0180001167.01.T01:CDS 40.0%
GAGATAGCAACTGATGTTGA+TGG + Chr1:16771407-16771426 MsG0180001167.01.T01:CDS 40.0%
GATCATCAAAACTCGTCACA+TGG + Chr1:16769446-16769465 MsG0180001167.01.T01:CDS 40.0%
GCATAAACTTTCCACAGGTA+AGG + Chr1:16771537-16771556 MsG0180001167.01.T01:CDS 40.0%
GCGCAGATTCCTATATTTGA+TGG + Chr1:16771341-16771360 MsG0180001167.01.T01:intron 40.0%
GTAAAGATGGTGTGAGGATT+AGG + Chr1:16769734-16769753 MsG0180001167.01.T01:CDS 40.0%
TCATCATCTCTTTGTGCAGA+CGG + Chr1:16769525-16769544 MsG0180001167.01.T01:CDS 40.0%
TCATTCCTCCAAACATGTAC+CGG - Chr1:16771470-16771489 None:intergenic 40.0%
TGAATGAACCGGTACATGTT+TGG + Chr1:16771459-16771478 MsG0180001167.01.T01:CDS 40.0%
TGAGCAAATTCAACAGAAGC+AGG - Chr1:16769688-16769707 None:intergenic 40.0%
TGCACATCTTGATTTCTGCA+AGG + Chr1:16770909-16770928 MsG0180001167.01.T01:intron 40.0%
! AAGGAAGGTGGTTTGAGTTT+AGG + Chr1:16769650-16769669 MsG0180001167.01.T01:CDS 40.0%
! AATCTCATTGCATTCTGGGA+AGG + Chr1:16769591-16769610 MsG0180001167.01.T01:CDS 40.0%
! GGATGACTTGATGATTCAAG+TGG + Chr1:16770206-16770225 MsG0180001167.01.T01:intron 40.0%
! TATATTTGATGGGTCGGCTA+GGG + Chr1:16771352-16771371 MsG0180001167.01.T01:CDS 40.0%
! TGACTTGATGATTCAAGTGG+GGG + Chr1:16770209-16770228 MsG0180001167.01.T01:intron 40.0%
!! AAGAGCATCCATACGCAAAA+TGG - Chr1:16770789-16770808 None:intergenic 40.0%
!! GTTCTGGGGTTGATAATTGT+AGG + Chr1:16769788-16769807 MsG0180001167.01.T01:CDS 40.0%
!!! CAGTTTTGAGAGGTTGTGTT+AGG + Chr1:16770411-16770430 MsG0180001167.01.T01:intron 40.0%
ACCACCTTCTATCAGGTTTC+CGG + Chr1:16770485-16770504 MsG0180001167.01.T01:intron 45.0%
AGATTGTGTCCTGGATTTGC+AGG + Chr1:16769625-16769644 MsG0180001167.01.T01:CDS 45.0%
AGCTCCACATGTGAATGAAC+CGG + Chr1:16771448-16771467 MsG0180001167.01.T01:CDS 45.0%
ATCATCTCTTTGTGCAGACG+GGG + Chr1:16769527-16769546 MsG0180001167.01.T01:CDS 45.0%
ATGAACCGGTACATGTTTGG+AGG + Chr1:16771462-16771481 MsG0180001167.01.T01:CDS 45.0%
CATCATCTCTTTGTGCAGAC+GGG + Chr1:16769526-16769545 MsG0180001167.01.T01:CDS 45.0%
CCACCTTAGAAATTGTGGAC+GGG - Chr1:16770437-16770456 None:intergenic 45.0%
CTAGCCGACCCATCAAATAT+AGG - Chr1:16771353-16771372 None:intergenic 45.0%
CTGATATGTTAGTCCCACGT+CGG + Chr1:16770340-16770359 MsG0180001167.01.T01:intron 45.0%
GCATTTCCTTCAGAAGTGGT+TGG + Chr1:16771503-16771522 MsG0180001167.01.T01:CDS 45.0%
GGAATCTGCGCATGAAATCA+AGG - Chr1:16771332-16771351 None:intergenic 45.0%
GTTACTCCAACCACTTCTGA+AGG - Chr1:16771512-16771531 None:intergenic 45.0%
TGCAGCATTTCCTTCAGAAG+TGG + Chr1:16771499-16771518 MsG0180001167.01.T01:CDS 45.0%
TTTCTGCGTTGGAAGGTTCT+GGG + Chr1:16769773-16769792 MsG0180001167.01.T01:CDS 45.0%
! CTATATTTGATGGGTCGGCT+AGG + Chr1:16771351-16771370 MsG0180001167.01.T01:CDS 45.0%
! CTCTAAGAGGAATTGTGGAG+CGG + Chr1:16769563-16769582 MsG0180001167.01.T01:CDS 45.0%
! GGAGCATTTACTTTCTGCGT+TGG + Chr1:16769762-16769781 MsG0180001167.01.T01:CDS 45.0%
!! CGGTTTTGTGAGGTTGTGTA+AGG + Chr1:16770597-16770616 MsG0180001167.01.T01:intron 45.0%
!! TGTACCGGTTCATTCACATG+TGG - Chr1:16771455-16771474 None:intergenic 45.0%
!!! AATCGTCACCCAGTTTTGAG+AGG + Chr1:16770401-16770420 MsG0180001167.01.T01:intron 45.0%
AAGAGGAATTGTGGAGCGGA+AGG + Chr1:16769567-16769586 MsG0180001167.01.T01:CDS 50.0%
AAGTCATCTCTCAACCGACG+TGG - Chr1:16770357-16770376 None:intergenic 50.0%
ACACCCGGAAACCTGATAGA+AGG - Chr1:16770492-16770511 None:intergenic 50.0%
AGAGGAATTGTGGAGCGGAA+GGG + Chr1:16769568-16769587 MsG0180001167.01.T01:CDS 50.0%
AGGCCATCTCCCAATCAATG+TGG - Chr1:16770535-16770554 None:intergenic 50.0%
AGGCCCGTCCACAATTTCTA+AGG + Chr1:16770431-16770450 MsG0180001167.01.T01:intron 50.0%
AGTCATCTCTCAACCGACGT+GGG - Chr1:16770356-16770375 None:intergenic 50.0%
CAAAGAGCGGAGTGATAAGG+AGG + Chr1:16770235-16770254 MsG0180001167.01.T01:intron 50.0%
CAAGTGGGGGAATCAAAGAG+CGG + Chr1:16770222-16770241 MsG0180001167.01.T01:intron 50.0%
CCACCTTCTATCAGGTTTCC+GGG + Chr1:16770486-16770505 MsG0180001167.01.T01:intron 50.0%
CCTGATAGAAGGTGGTCCAA+CGG - Chr1:16770481-16770500 None:intergenic 50.0%
CTGATAGAAGGTGGTCCAAC+GGG - Chr1:16770480-16770499 None:intergenic 50.0%
CTGGATTTGCAGGTCAAGGA+AGG + Chr1:16769635-16769654 MsG0180001167.01.T01:CDS 50.0%
CTTCCTTGACCTGCAAATCC+AGG - Chr1:16769637-16769656 None:intergenic 50.0%
CTTTCTGCGTTGGAAGGTTC+TGG + Chr1:16769772-16769791 MsG0180001167.01.T01:CDS 50.0%
GAAATCCTCACCTCACAAGC+CGG + Chr1:16770577-16770596 MsG0180001167.01.T01:intron 50.0%
GATTTGCAGGTCAAGGAAGG+TGG + Chr1:16769638-16769657 MsG0180001167.01.T01:CDS 50.0%
GGCCATCTCCCAATCAATGT+GGG - Chr1:16770534-16770553 None:intergenic 50.0%
TGGTGTGAGGATTAGGACTG+TGG + Chr1:16769741-16769760 MsG0180001167.01.T01:CDS 50.0%
TGTCCTGGATTTGCAGGTCA+AGG + Chr1:16769631-16769650 MsG0180001167.01.T01:CDS 50.0%
TTCTGCGTTGGAAGGTTCTG+GGG + Chr1:16769774-16769793 MsG0180001167.01.T01:CDS 50.0%
TTTAACACCCCTCTCACACC+CGG - Chr1:16770507-16770526 None:intergenic 50.0%
! CCCGTCCACAATTTCTAAGG+TGG + Chr1:16770434-16770453 MsG0180001167.01.T01:intron 50.0%
! GTCCCACATTGATTGGGAGA+TGG + Chr1:16770529-16770548 MsG0180001167.01.T01:intron 50.0%
! TGATCAAGGTGATGATGCCG+AGG + Chr1:16769831-16769850 MsG0180001167.01.T01:CDS 50.0%
!! GCATTCTGGGAAGGTATCGA+CGG + Chr1:16769600-16769619 MsG0180001167.01.T01:CDS 50.0%
ATCAGGTTTCCGGGTGTGAG+AGG + Chr1:16770495-16770514 MsG0180001167.01.T01:intron 55.0%
CCCGGAAACCTGATAGAAGG+TGG - Chr1:16770489-16770508 None:intergenic 55.0%
CCGTTGGACCACCTTCTATC+AGG + Chr1:16770478-16770497 MsG0180001167.01.T01:intron 55.0%
GTGGCAGCCTTGGAAGAAGT+TGG + Chr1:16771380-16771399 MsG0180001167.01.T01:CDS 55.0%
TCAGGTTTCCGGGTGTGAGA+GGG + Chr1:16770496-16770515 MsG0180001167.01.T01:intron 55.0%
TCGACGGTGAGATTGTGTCC+TGG + Chr1:16769616-16769635 MsG0180001167.01.T01:CDS 55.0%
! TGATGGGTCGGCTAGGGAAT+GGG + Chr1:16771358-16771377 MsG0180001167.01.T01:CDS 55.0%
! TTGATGGGTCGGCTAGGGAA+TGG + Chr1:16771357-16771376 MsG0180001167.01.T01:CDS 55.0%
!! CAAAACCGGCTTGTGAGGTG+AGG - Chr1:16770585-16770604 None:intergenic 55.0%
!! CCTCACAAAACCGGCTTGTG+AGG - Chr1:16770590-16770609 None:intergenic 55.0%
!! CCTCACAAGCCGGTTTTGTG+AGG + Chr1:16770587-16770606 MsG0180001167.01.T01:intron 55.0%
CAGGTTTCCGGGTGTGAGAG+GGG + Chr1:16770497-16770516 MsG0180001167.01.T01:intron 60.0%
GAAGGTGGTCCAACGGGTCT+TGG - Chr1:16770474-16770493 None:intergenic 60.0%
TAGGGAATGGGTGGCAGCCT+TGG + Chr1:16771370-16771389 MsG0180001167.01.T01:CDS 60.0%
GAGCCTCCTCCAAGACCCGT+TGG + Chr1:16770462-16770481 MsG0180001167.01.T01:intron 65.0%
GGTCCAACGGGTCTTGGAGG+AGG - Chr1:16770468-16770487 None:intergenic 65.0%
GGTGGTCCAACGGGTCTTGG+AGG - Chr1:16770471-16770490 None:intergenic 65.0%
TGGGTCGGCTAGGGAATGGG+TGG + Chr1:16771361-16771380 MsG0180001167.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 16769424 16771568 16769424 ID=MsG0180001167.01;Name=MsG0180001167.01
Chr1 mRNA 16769424 16771568 16769424 ID=MsG0180001167.01.T01;Parent=MsG0180001167.01;Name=MsG0180001167.01.T01;_AED=0.44;_eAED=0.44;_QI=0|1|0|1|1|1|2|0|216
Chr1 exon 16769424 16769852 16769424 ID=MsG0180001167.01.T01:exon:31484;Parent=MsG0180001167.01.T01
Chr1 exon 16771347 16771568 16771347 ID=MsG0180001167.01.T01:exon:31485;Parent=MsG0180001167.01.T01
Chr1 CDS 16769424 16769852 16769424 ID=MsG0180001167.01.T01:cds;Parent=MsG0180001167.01.T01
Chr1 CDS 16771347 16771568 16771347 ID=MsG0180001167.01.T01:cds;Parent=MsG0180001167.01.T01
Gene Sequence

>MsG0180001167.01.T01

ATGTCTCTTCTCAGCGCTCTCAGATCATCAAAACTCGTCACATGGAAACCCGTAACTCACTCTCTCTTATACCCTGTTTGCTTCATTTCAAATTTCTATTCTCATCATCTCTTTGTGCAGACGGGGAAGCTTCAACAAACTCTAAGAGGAATTGTGGAGCGGAAGGGAATCTCATTGCATTCTGGGAAGGTATCGACGGTGAGATTGTGTCCTGGATTTGCAGGTCAAGGAAGGTGGTTTGAGTTTAGGAACAATTTGGTTCCTGCTTCTGTTGAATTTGCTCAAGTTTCACCGCTTTGTACTACTTTGTGTAAAGATGGTGTGAGGATTAGGACTGTGGAGCATTTACTTTCTGCGTTGGAAGGTTCTGGGGTTGATAATTGTAGGATTCAGATTGAAAATTTGGATGATCAAGGTGATGATGCCGAGATTCCTATATTTGATGGGTCGGCTAGGGAATGGGTGGCAGCCTTGGAAGAAGTTGGTTTAGAGATAGCAACTGATGTTGATGGAAAAAGCTGTGAGAAAATAGCTCCACATGTGAATGAACCGGTACATGTTTGGAGGAATGATTCTTTTGTTGCAGCATTTCCTTCAGAAGTGGTTGGAGTAACTTATGGCATAAACTTTCCACAGGTAAGGATAGGTTGA
Protein sequence

>MsG0180001167.01.T01

MSLLSALRSSKLVTWKPVTHSLLYPVCFISNFYSHHLFVQTGKLQQTLRGIVERKGISLHSGKVSTVRLCPGFAGQGRWFEFRNNLVPASVEFAQVSPLCTTLCKDGVRIRTVEHLLSALEGSGVDNCRIQIENLDDQGDDAEIPIFDGSAREWVAALEEVGLEIATDVDGKSCEKIAPHVNEPVHVWRNDSFVAAFPSEVVGVTYGINFPQVRIG*