AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031939.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031939.01.T01 MTR_5g067140 96.078 102 4 0 72 173 579 680 7.02e-61 201
MsG0680031939.01.T01 MTR_5g067140 75.238 105 19 2 1 105 159 256 1.43e-45 159
MsG0680031939.01.T01 MTR_1g103840 32.558 129 76 3 5 130 258 378 1.47e-15 73.9
MsG0680031939.01.T01 MTR_2g086150 38.384 99 60 1 73 170 679 777 1.96e-15 73.6
MsG0680031939.01.T01 MTR_1g007610 28.218 202 111 3 1 169 377 577 4.88e-15 72.4
MsG0680031939.01.T01 MTR_4g088840 28.643 199 108 5 5 169 302 500 8.60e-14 68.9
MsG0680031939.01.T01 MTR_7g037430 26.368 201 114 4 3 170 663 862 1.69e-13 68.2
MsG0680031939.01.T01 MTR_1g073300 36.364 99 62 1 72 169 606 704 3.04e-13 67.4
MsG0680031939.01.T01 MTR_7g076707 32.520 123 70 3 5 123 293 406 6.31e-13 66.2
MsG0680031939.01.T01 MTR_7g076350 26.368 201 114 4 3 170 398 597 8.30e-13 65.9
MsG0680031939.01.T01 MTR_8g107740 36.275 102 61 1 5 106 295 392 1.25e-12 65.5
MsG0680031939.01.T01 MTR_1g059720 33.333 99 65 1 72 169 384 482 1.32e-12 65.5
MsG0680031939.01.T01 MTR_1g103660 28.079 203 104 5 5 169 395 593 1.74e-12 65.1
MsG0680031939.01.T01 MTR_1g111020 39.189 74 45 0 73 146 672 745 1.77e-12 65.1
MsG0680031939.01.T01 MTR_5g088310 28.079 203 104 5 5 169 395 593 2.03e-12 64.7
MsG0680031939.01.T01 MTR_1g111020 39.189 74 45 0 73 146 672 745 2.71e-12 64.7
MsG0680031939.01.T01 MTR_7g417770 29.121 182 90 6 5 151 659 836 3.28e-12 64.3
MsG0680031939.01.T01 MTR_7g017700 29.121 182 90 6 5 151 659 836 3.28e-12 64.3
MsG0680031939.01.T01 MTR_4g031160 34.694 98 63 1 73 169 651 748 3.35e-12 64.3
MsG0680031939.01.T01 MTR_4g031160 31.373 102 66 1 5 106 245 342 9.48e-11 60.1
MsG0680031939.01.T01 MTR_8g065730 26.768 198 111 5 5 169 461 657 4.14e-12 63.9
MsG0680031939.01.T01 MTR_8g069550 26.768 198 111 5 5 169 470 666 4.31e-12 63.9
MsG0680031939.01.T01 MTR_7g084830 34.615 130 72 6 2 128 232 351 5.27e-12 63.5
MsG0680031939.01.T01 MTR_2g009760 35.644 101 56 3 5 105 359 450 8.92e-12 62.8
MsG0680031939.01.T01 MTR_1g040635 36.111 108 66 2 65 169 539 646 1.03e-11 62.8
MsG0680031939.01.T01 MTR_1g040585 36.735 98 61 1 73 169 441 538 1.61e-11 62.0
MsG0680031939.01.T01 MTR_1g040585 29.839 124 78 5 4 123 236 354 6.82e-11 60.5
MsG0680031939.01.T01 MTR_1g040565 29.839 124 78 5 4 123 179 297 2.35e-11 61.6
MsG0680031939.01.T01 MTR_1g040565 36.735 98 61 1 73 169 384 481 2.35e-11 61.6
MsG0680031939.01.T01 MTR_3g098230 35.000 100 58 2 6 105 130 222 3.03e-11 61.6
MsG0680031939.01.T01 MTR_8g106950 28.155 103 73 1 68 169 573 675 4.32e-11 60.8
MsG0680031939.01.T01 MTR_2g021710 29.412 119 76 2 2 118 33 145 4.62e-11 60.8
MsG0680031939.01.T01 MTR_5g023040 34.177 79 52 0 73 151 640 718 5.59e-11 60.8
MsG0680031939.01.T01 MTR_8g087980 29.365 126 74 3 46 160 380 501 8.52e-11 60.1
MsG0680031939.01.T01 MTR_4g094692 35.294 102 63 2 72 170 658 759 8.91e-11 60.1
MsG0680031939.01.T01 MTR_8g063450 35.000 80 52 0 72 151 578 657 9.93e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031939.01.T01 AT2G27610 36.364 110 65 2 62 170 672 777 1.51e-16 77.0
MsG0680031939.01.T01 AT5G16860 31.538 130 84 2 2 130 209 334 6.83e-14 69.3
MsG0680031939.01.T01 AT4G14850 30.827 133 84 3 42 170 466 594 3.69e-13 67.0
MsG0680031939.01.T01 AT5G09950 25.248 202 116 3 3 169 696 897 4.14e-13 67.0
MsG0680031939.01.T01 AT5G09950 25.248 202 116 3 3 169 702 903 4.26e-13 67.0
MsG0680031939.01.T01 AT5G09950 25.248 202 116 3 3 169 702 903 4.26e-13 67.0
MsG0680031939.01.T01 AT1G25360 35.354 99 63 1 72 169 600 698 4.85e-13 66.6
MsG0680031939.01.T01 AT4G02750 31.068 103 70 1 68 169 588 690 6.90e-13 66.2
MsG0680031939.01.T01 AT2G22070 30.769 130 79 4 4 130 261 382 7.04e-13 66.2
MsG0680031939.01.T01 AT3G24000 34.694 98 63 1 73 169 445 542 1.88e-12 65.1
MsG0680031939.01.T01 AT2G29760 30.081 123 80 2 2 123 111 228 9.48e-12 63.2
MsG0680031939.01.T01 AT3G22690 28.824 170 116 4 3 169 553 720 1.13e-11 62.8
MsG0680031939.01.T01 AT3G62890 42.466 73 38 1 3 71 176 248 2.13e-11 62.0
MsG0680031939.01.T01 AT5G40410 32.110 109 68 3 64 170 414 518 4.25e-11 60.8
MsG0680031939.01.T01 AT4G33170 30.392 102 70 1 69 169 798 899 4.56e-11 61.2
MsG0680031939.01.T01 AT5G66520 31.746 126 75 4 1 124 227 343 6.68e-11 60.5
MsG0680031939.01.T01 AT4G16835 39.189 74 45 0 73 146 468 541 7.36e-11 60.5
MsG0680031939.01.T01 AT5G52850 31.132 106 68 2 5 110 108 208 7.38e-11 60.5
MsG0680031939.01.T01 AT1G77010 34.783 115 67 4 1 114 299 406 9.26e-11 60.1

Find 25 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCATCACAACCCAATTC+TGG 0.256144 6:-30198754 None:intergenic
TTGTATGTCTCTGTATCTTT+TGG 0.285450 6:-30198799 None:intergenic
AACAAACGTATAGCATGTTT+TGG 0.297648 6:-30198244 None:intergenic
GACAATATGACGATGTTTCT+AGG 0.297649 6:+30198851 MsG0680031939.01.T01:CDS
TCGTTGCATTCTTTGAAGTT+AGG 0.317169 6:+30198340 MsG0680031939.01.T01:CDS
CATATCAAAGCAAAGAATCT+TGG 0.347447 6:+30199015 MsG0680031939.01.T01:CDS
ATAATGAAAGAAGAGAAAAT+TGG 0.380442 6:+30198883 MsG0680031939.01.T01:CDS
TAAGCCAGGGAATCCAGAAT+TGG 0.394526 6:+30198741 MsG0680031939.01.T01:intron
GCAGACATAGAAAGTTCAAT+AGG 0.414227 6:+30198973 MsG0680031939.01.T01:CDS
GAGAGTTAAAAGATTGGAGC+TGG 0.445621 6:+30198905 MsG0680031939.01.T01:CDS
AAATTGGAGAGTTAAAAGAT+TGG 0.448958 6:+30198899 MsG0680031939.01.T01:CDS
TTCAATAGGTAAATCATTGG+AGG 0.504581 6:+30198987 MsG0680031939.01.T01:CDS
ATAGCAAGAGTGTAATTTGA+AGG 0.508705 6:-30198298 None:intergenic
AAGCCAGGGAATCCAGAATT+GGG 0.516437 6:+30198742 MsG0680031939.01.T01:intron
AGAAACATCGTCATATTGTC+CGG 0.529669 6:-30198848 None:intergenic
AAGTTCAATAGGTAAATCAT+TGG 0.535449 6:+30198984 MsG0680031939.01.T01:CDS
TGGAATGCGTACGTCTCAGC+CGG 0.545891 6:+30198829 MsG0680031939.01.T01:CDS
TGTAGTTTATACACTAAATG+TGG 0.554366 6:+30198430 MsG0680031939.01.T01:CDS
ACAATATGACGATGTTTCTA+GGG 0.557983 6:+30198852 MsG0680031939.01.T01:CDS
TAGCAAGAGTGTAATTTGAA+GGG 0.603105 6:-30198297 None:intergenic
GATATGAGATGTTAGAAGTG+TGG 0.605394 6:+30199037 MsG0680031939.01.T01:CDS
AGTTTATACACTAAATGTGG+TGG 0.635862 6:+30198433 MsG0680031939.01.T01:CDS
CAGAGACATACAAATTGCTG+TGG 0.636317 6:+30198809 MsG0680031939.01.T01:CDS
ATATGACGATGTTTCTAGGG+TGG 0.639941 6:+30198855 MsG0680031939.01.T01:CDS
TACTTGTGTCAAAATCAACA+TGG 0.692603 6:-30198393 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAAATTAACCTGTGA+TGG + Chr6:30198593-30198612 MsG0680031939.01.T01:intron 20.0%
!! ATAATGAAAGAAGAGAAAAT+TGG + Chr6:30198883-30198902 MsG0680031939.01.T01:CDS 20.0%
! AAATTGGAGAGTTAAAAGAT+TGG + Chr6:30198899-30198918 MsG0680031939.01.T01:CDS 25.0%
! AAGTTCAATAGGTAAATCAT+TGG + Chr6:30198984-30199003 MsG0680031939.01.T01:CDS 25.0%
! ATTTAGGAGAATCAAAGAAA+AGG + Chr6:30198480-30198499 MsG0680031939.01.T01:intron 25.0%
! CTTTCGATTGATAAAGAAAA+TGG + Chr6:30198670-30198689 MsG0680031939.01.T01:intron 25.0%
! TGTAGTTTATACACTAAATG+TGG + Chr6:30198430-30198449 MsG0680031939.01.T01:CDS 25.0%
!!! AAGAAAAGGATGTTATTTCT+TGG + Chr6:30198494-30198513 MsG0680031939.01.T01:intron 25.0%
!!! GATAAAGAAAATGGATTATG+AGG + Chr6:30198679-30198698 MsG0680031939.01.T01:intron 25.0%
!!! TTACTTTGAGATTATGCAAA+AGG + Chr6:30198570-30198589 MsG0680031939.01.T01:intron 25.0%
AGTTTATACACTAAATGTGG+TGG + Chr6:30198433-30198452 MsG0680031939.01.T01:CDS 30.0%
ATTTGGTCAAATTTGATTGC+TGG + Chr6:30198713-30198732 MsG0680031939.01.T01:intron 30.0%
CATATCAAAGCAAAGAATCT+TGG + Chr6:30199015-30199034 MsG0680031939.01.T01:CDS 30.0%
TACTTGTGTCAAAATCAACA+TGG - Chr6:30198396-30198415 None:intergenic 30.0%
TATCAACCAAACAAGCATAA+TGG - Chr6:30198620-30198639 None:intergenic 30.0%
TTCAATAGGTAAATCATTGG+AGG + Chr6:30198987-30199006 MsG0680031939.01.T01:CDS 30.0%
! AACAAACGTATAGCATGTTT+TGG - Chr6:30198247-30198266 None:intergenic 30.0%
! ACAATATGACGATGTTTCTA+GGG + Chr6:30198852-30198871 MsG0680031939.01.T01:CDS 30.0%
! ATAGCAAGAGTGTAATTTGA+AGG - Chr6:30198301-30198320 None:intergenic 30.0%
! TAGCAAGAGTGTAATTTGAA+GGG - Chr6:30198300-30198319 None:intergenic 30.0%
! TTGTATGTCTCTGTATCTTT+TGG - Chr6:30198802-30198821 None:intergenic 30.0%
!! ACTTTGGTCTAAAAGCATTT+AGG + Chr6:30198464-30198483 MsG0680031939.01.T01:intron 30.0%
!! GTTGATTTTGACACAAGTAT+CGG + Chr6:30198397-30198416 MsG0680031939.01.T01:CDS 30.0%
AGAAACATCGTCATATTGTC+CGG - Chr6:30198851-30198870 None:intergenic 35.0%
ATGAGGCTAGCAAGTTTATT+TGG + Chr6:30198696-30198715 MsG0680031939.01.T01:intron 35.0%
GACAATATGACGATGTTTCT+AGG + Chr6:30198851-30198870 MsG0680031939.01.T01:CDS 35.0%
GATATGAGATGTTAGAAGTG+TGG + Chr6:30199037-30199056 MsG0680031939.01.T01:CDS 35.0%
GCAGACATAGAAAGTTCAAT+AGG + Chr6:30198973-30198992 MsG0680031939.01.T01:CDS 35.0%
! GTTTGGTTGATATGTTAGTG+AGG + Chr6:30198628-30198647 MsG0680031939.01.T01:intron 35.0%
!! TCGTTGCATTCTTTGAAGTT+AGG + Chr6:30198340-30198359 MsG0680031939.01.T01:CDS 35.0%
CACTAAATGTGGTGGTAAGT+TGG + Chr6:30198441-30198460 MsG0680031939.01.T01:intron 40.0%
CAGAGACATACAAATTGCTG+TGG + Chr6:30198809-30198828 MsG0680031939.01.T01:CDS 40.0%
GAGAGTTAAAAGATTGGAGC+TGG + Chr6:30198905-30198924 MsG0680031939.01.T01:CDS 40.0%
GATGGACCATTATGCTTGTT+TGG + Chr6:30198611-30198630 MsG0680031939.01.T01:intron 40.0%
GTTGATATGTTAGTGAGGCT+CGG + Chr6:30198633-30198652 MsG0680031939.01.T01:intron 40.0%
!! ATATGACGATGTTTCTAGGG+TGG + Chr6:30198855-30198874 MsG0680031939.01.T01:CDS 40.0%
AAGCCAGGGAATCCAGAATT+GGG + Chr6:30198742-30198761 MsG0680031939.01.T01:intron 45.0%
ATATGTTAGTGAGGCTCGGA+CGG + Chr6:30198637-30198656 MsG0680031939.01.T01:intron 45.0%
CAAGCATAATGGTCCATCAC+AGG - Chr6:30198609-30198628 None:intergenic 45.0%
TAAGCCAGGGAATCCAGAAT+TGG + Chr6:30198741-30198760 MsG0680031939.01.T01:intron 45.0%
! ATTGCTGGTTGTCTAAGCCA+GGG + Chr6:30198728-30198747 MsG0680031939.01.T01:intron 45.0%
! TGTGGTGGTAAGTTGGACTT+TGG + Chr6:30198448-30198467 MsG0680031939.01.T01:intron 45.0%
!! ACTGCAGCTATTTCTGCATG+CGG + Chr6:30198517-30198536 MsG0680031939.01.T01:intron 45.0%
CAACCCAATTCTGGATTCCC+TGG - Chr6:30198748-30198767 None:intergenic 50.0%
GCAGCATCACAACCCAATTC+TGG - Chr6:30198757-30198776 None:intergenic 50.0%
TATGTTAGTGAGGCTCGGAC+GGG + Chr6:30198638-30198657 MsG0680031939.01.T01:intron 50.0%
! GATTGCTGGTTGTCTAAGCC+AGG + Chr6:30198727-30198746 MsG0680031939.01.T01:intron 50.0%
TGGAATGCGTACGTCTCAGC+CGG + Chr6:30198829-30198848 MsG0680031939.01.T01:CDS 55.0%
AGTGAGGCTCGGACGGGTAG+AGG + Chr6:30198644-30198663 MsG0680031939.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 30198241 30199065 30198241 ID=MsG0680031939.01;Name=MsG0680031939.01
Chr6 mRNA 30198241 30199065 30198241 ID=MsG0680031939.01.T01;Parent=MsG0680031939.01;Name=MsG0680031939.01.T01;_AED=0.31;_eAED=0.33;_QI=0|0|0|1|1|1|2|0|176
Chr6 exon 30198241 30198454 30198241 ID=MsG0680031939.01.T01:exon:15481;Parent=MsG0680031939.01.T01
Chr6 exon 30198749 30199065 30198749 ID=MsG0680031939.01.T01:exon:15480;Parent=MsG0680031939.01.T01
Chr6 CDS 30198241 30198454 30198241 ID=MsG0680031939.01.T01:cds;Parent=MsG0680031939.01.T01
Chr6 CDS 30198749 30199065 30198749 ID=MsG0680031939.01.T01:cds;Parent=MsG0680031939.01.T01
Gene Sequence

>MsG0680031939.01.T01

ATGCCAAAACATGCTATACGTTTGTTTGAAGAGATGCTGTTGCATTCAGAGTGTTACCCTTCAAATTACACTCTTGCTATAGTCGTTAATGCTTGTACATCGTTGCATTCTTTGAAGTTAGGAGAACAGTTACATGCTTACATAATCAAATACCATGTTGATTTTGACACAAGTATCGGCAATGCGCTTTGTAGTTTATACACTAAATGTGGTGGGAATCCAGAATTGGGTTGTGATGCTGCAGAAAAGTTGTTAAGTCTCAAACCAAAAGATACAGAGACATACAAATTGCTGTGGAATGCGTACGTCTCAGCCGGACAATATGACGATGTTTCTAGGGTGGAGAATATAATGAAAGAAGAGAAAATTGGAGAGTTAAAAGATTGGAGCTGGATTAGCATCAAAGACAGAGTGTATTCATTTCAAACAAATGACAAAGCAGACATAGAAAGTTCAATAGGTAAATCATTGGAGGATTTACATATCAAAGCAAAGAATCTTGGATATGAGATGTTAGAAGTGTGGAAATAA

Protein sequence

>MsG0680031939.01.T01

MPKHAIRLFEEMLLHSECYPSNYTLAIVVNACTSLHSLKLGEQLHAYIIKYHVDFDTSIGNALCSLYTKCGGNPELGCDAAEKLLSLKPKDTETYKLLWNAYVSAGQYDDVSRVENIMKEEKIGELKDWSWISIKDRVYSFQTNDKADIESSIGKSLEDLHIKAKNLGYEMLEVWK*