Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023448.01.T01 | XP_039684597.1 | 96.774 | 62 | 2 | 0 | 2 | 63 | 26 | 87 | 2.39E-32 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023448.01.T01 | Q8S8Y8 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.23E-26 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023448.01.T01 | A0A072TUS1 | 96.774 | 62 | 2 | 0 | 2 | 63 | 62 | 123 | 1.38e-32 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048849.01 | MsG0480023448.01 | 0.827538 | 1.480163e-54 | 1.433059e-51 |
| MsG0080048878.01 | MsG0480023448.01 | 0.809844 | 1.547811e-50 | 9.186044e-48 |
| MsG0080049069.01 | MsG0480023448.01 | 0.814248 | 1.696911e-51 | 1.132770e-48 |
| MsG0080049147.01 | MsG0480023448.01 | 0.812077 | 5.082338e-51 | 3.200065e-48 |
| MsG0180000355.01 | MsG0480023448.01 | 0.812656 | 3.799045e-51 | 2.429373e-48 |
| MsG0180001167.01 | MsG0480023448.01 | 0.810645 | 1.039770e-50 | 6.302332e-48 |
| MsG0180003697.01 | MsG0480023448.01 | 0.850317 | 1.835721e-60 | 3.581868e-57 |
| MsG0180003821.01 | MsG0480023448.01 | 0.803487 | 3.405621e-49 | 1.713565e-46 |
| MsG0180004750.01 | MsG0480023448.01 | 0.825870 | 3.701656e-54 | 3.416049e-51 |
| MsG0180005193.01 | MsG0480023448.01 | 0.816650 | 4.957499e-52 | 3.533307e-49 |
| MsG0180005303.01 | MsG0480023448.01 | -0.815767 | 7.807532e-52 | 5.432166e-49 |
| MsG0180006096.01 | MsG0480023448.01 | 0.833043 | 6.690146e-56 | 7.614508e-53 |
| MsG0180006144.01 | MsG0480023448.01 | 0.831835 | 1.332151e-55 | 1.462912e-52 |
| MsG0180006220.01 | MsG0480023448.01 | 0.821139 | 4.731257e-53 | 3.817424e-50 |
| MsG0280006306.01 | MsG0480023448.01 | -0.804976 | 1.667792e-49 | 8.719716e-47 |
| MsG0280011304.01 | MsG0480023448.01 | 0.854311 | 1.339343e-61 | 2.978657e-58 |
| MsG0280011371.01 | MsG0480023448.01 | 0.810420 | 1.163153e-50 | 7.007643e-48 |
| MsG0380012005.01 | MsG0480023448.01 | 0.813943 | 1.981375e-51 | 1.311706e-48 |
| MsG0380012122.01 | MsG0480023448.01 | 0.805416 | 1.348980e-49 | 7.132788e-47 |
| MsG0380013467.01 | MsG0480023448.01 | 0.841951 | 3.479718e-58 | 5.195519e-55 |
| MsG0380013853.01 | MsG0480023448.01 | 0.807059 | 6.081370e-50 | 3.353993e-47 |
| MsG0480018911.01 | MsG0480023448.01 | 0.818883 | 1.553165e-52 | 1.177047e-49 |
| MsG0480020024.01 | MsG0480023448.01 | 0.829441 | 5.139318e-55 | 5.260285e-52 |
| MsG0480021280.01 | MsG0480023448.01 | 0.808165 | 3.541556e-50 | 2.010571e-47 |
| MsG0480021768.01 | MsG0480023448.01 | 0.813920 | 2.005117e-51 | 1.326599e-48 |
| MsG0480021776.01 | MsG0480023448.01 | -0.830988 | 2.153125e-55 | 2.305593e-52 |
| MsG0480023073.01 | MsG0480023448.01 | 0.817127 | 3.873596e-52 | 2.797005e-49 |
| MsG0480023162.01 | MsG0480023448.01 | 0.818049 | 2.400286e-52 | 1.777485e-49 |
| MsG0480023448.01 | MsG0480023652.01 | 0.840552 | 8.118208e-58 | 1.160505e-54 |
| MsG0480023448.01 | MsG0480023660.01 | 0.839419 | 1.602930e-57 | 2.212388e-54 |
| MsG0480023448.01 | MsG0580024495.01 | 0.823355 | 1.448759e-53 | 1.244385e-50 |
| MsG0480023448.01 | MsG0580025467.01 | 0.800647 | 1.306694e-48 | 6.116664e-46 |
| MsG0480023448.01 | MsG0580027103.01 | 0.813148 | 2.964004e-51 | 1.920277e-48 |
| MsG0480023448.01 | MsG0580028044.01 | 0.817153 | 3.822633e-52 | 2.762216e-49 |
| MsG0480023448.01 | MsG0580028630.01 | 0.811542 | 6.644652e-51 | 4.124473e-48 |
| MsG0480023448.01 | MsG0580029717.01 | 0.811247 | 7.700486e-51 | 4.742683e-48 |
| MsG0480023448.01 | MsG0580029879.01 | 0.810195 | 1.300340e-50 | 7.788054e-48 |
| MsG0480023448.01 | MsG0580029925.01 | 0.816165 | 6.366124e-52 | 4.477337e-49 |
| MsG0480023448.01 | MsG0680030356.01 | -0.804540 | 2.056892e-49 | 1.063306e-46 |
| MsG0480023448.01 | MsG0680030555.01 | 0.806040 | 9.975149e-50 | 5.359682e-47 |
| MsG0480023448.01 | MsG0680030636.01 | 0.822909 | 1.840734e-53 | 1.561473e-50 |
| MsG0480023448.01 | MsG0680030764.01 | 0.807747 | 4.346352e-50 | 2.440426e-47 |
| MsG0480023448.01 | MsG0680030992.01 | 0.806538 | 7.834455e-50 | 4.263270e-47 |
| MsG0480023448.01 | MsG0680031939.01 | 0.813380 | 2.636028e-51 | 1.718778e-48 |
| MsG0480023448.01 | MsG0680032827.01 | 0.810518 | 1.107572e-50 | 6.690427e-48 |
| MsG0480023448.01 | MsG0680033459.01 | 0.822725 | 2.031150e-53 | 1.714084e-50 |
| MsG0480023448.01 | MsG0680035765.01 | 0.812294 | 4.557076e-51 | 2.886387e-48 |
| MsG0480023448.01 | MsG0680035877.01 | 0.807081 | 6.017618e-50 | 3.320619e-47 |
| MsG0480023448.01 | MsG0780035957.01 | 0.822079 | 2.869020e-53 | 2.376795e-50 |
| MsG0480023448.01 | MsG0780035960.01 | 0.825642 | 4.192859e-54 | 3.844082e-51 |
| MsG0480023448.01 | MsG0780036071.01 | 0.816042 | 6.781442e-52 | 4.753687e-49 |
| MsG0480023448.01 | MsG0780036186.01 | 0.805557 | 1.260620e-49 | 6.689511e-47 |
| MsG0480023448.01 | MsG0780037082.01 | 0.810013 | 1.423577e-50 | 8.486531e-48 |
| MsG0480023448.01 | MsG0780039438.01 | 0.801923 | 7.161579e-49 | 3.462286e-46 |
| MsG0480023448.01 | MsG0780040596.01 | 0.817035 | 4.063056e-52 | 2.926519e-49 |
| MsG0480023448.01 | MsG0780041729.01 | -0.801459 | 8.917122e-49 | 4.260674e-46 |
| MsG0480023448.01 | MsG0780041747.01 | 0.828413 | 9.115307e-55 | 9.054195e-52 |
| MsG0480023448.01 | MsG0880042134.01 | 0.805159 | 1.526825e-49 | 8.020134e-47 |
| MsG0480023448.01 | MsG0880042700.01 | 0.800018 | 1.754638e-48 | 8.083324e-46 |
| MsG0480023448.01 | MsG0880045765.01 | 0.802369 | 5.795795e-49 | 2.834246e-46 |
| MsG0480023448.01 | MsG0880046963.01 | -0.818497 | 1.901083e-52 | 1.425354e-49 |
| MsG0480023448.01 | MsG0880047481.01 | -0.800736 | 1.253072e-48 | 5.878891e-46 |
| MsG0480023448.01 | MsG0880047763.01 | 0.811775 | 5.912968e-51 | 3.692892e-48 |
| MsG0380015216.01 | MsG0480023448.01 | 0.820942 | 5.252174e-53 | 4.214686e-50 |
| MsG0380015533.01 | MsG0480023448.01 | 0.842088 | 3.200322e-58 | 4.799492e-55 |
| MsG0380015703.01 | MsG0480023448.01 | 0.819090 | 1.393916e-52 | 1.062364e-49 |
| MsG0380016191.01 | MsG0480023448.01 | -0.809466 | 1.866437e-50 | 1.096646e-47 |
| MsG0380016820.01 | MsG0480023448.01 | 0.810245 | 1.268535e-50 | 7.607908e-48 |
| MsG0380017023.01 | MsG0480023448.01 | 0.828934 | 6.822732e-55 | 6.879569e-52 |
| MsG0380017085.01 | MsG0480023448.01 | 0.861237 | 1.181150e-63 | 3.319231e-60 |
| MsG0380017126.01 | MsG0480023448.01 | 0.807863 | 4.106866e-50 | 2.312800e-47 |
| MsG0380017891.01 | MsG0480023448.01 | -0.805621 | 1.221753e-49 | 6.494205e-47 |
| MsG0480018402.01 | MsG0480023448.01 | 0.805031 | 1.624208e-49 | 8.503428e-47 |
| MsG0280007193.01 | MsG0480023448.01 | -0.803759 | 2.990081e-49 | 1.515073e-46 |
| MsG0280007763.01 | MsG0480023448.01 | 0.801972 | 6.995745e-49 | 3.386245e-46 |
| MsG0280007903.01 | MsG0480023448.01 | 0.817937 | 2.544591e-52 | 1.878616e-49 |
| MsG0280008928.01 | MsG0480023448.01 | 0.817383 | 3.393503e-52 | 2.467720e-49 |
| MsG0280008929.01 | MsG0480023448.01 | 0.819421 | 1.171649e-52 | 9.011997e-50 |
| MsG0280009256.01 | MsG0480023448.01 | 0.817931 | 2.552562e-52 | 1.884246e-49 |
| MsG0280010434.01 | MsG0480023448.01 | 0.803864 | 2.844696e-49 | 1.445294e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023448.01.T01 | MTR_8g093090 | 96.774 | 62 | 2 | 0 | 2 | 63 | 62 | 123 | 3.49e-36 | 127 |
| MsG0480023448.01.T01 | MTR_8g093090 | 96.774 | 62 | 2 | 0 | 2 | 63 | 80 | 141 | 3.69e-36 | 127 |
| MsG0480023448.01.T01 | MTR_3g095680 | 81.667 | 60 | 11 | 0 | 4 | 63 | 96 | 155 | 1.53e-29 | 108 |
| MsG0480023448.01.T01 | MTR_3g095680 | 81.667 | 60 | 11 | 0 | 4 | 63 | 64 | 123 | 1.56e-29 | 108 |
| MsG0480023448.01.T01 | MTR_8g027265 | 68.333 | 60 | 19 | 0 | 4 | 63 | 69 | 128 | 4.63e-24 | 93.2 |
| MsG0480023448.01.T01 | MTR_8g093090 | 100.000 | 41 | 0 | 0 | 23 | 63 | 1 | 41 | 5.89e-23 | 90.1 |
| MsG0480023448.01.T01 | MTR_3g080960 | 61.667 | 60 | 23 | 0 | 4 | 63 | 51 | 110 | 6.84e-23 | 89.7 |
| MsG0480023448.01.T01 | MTR_3g076650 | 56.667 | 60 | 26 | 0 | 4 | 63 | 57 | 116 | 6.03e-21 | 84.3 |
| MsG0480023448.01.T01 | MTR_1g081330 | 56.667 | 60 | 26 | 0 | 4 | 63 | 55 | 114 | 1.42e-20 | 83.2 |
| MsG0480023448.01.T01 | MTR_7g114680 | 55.000 | 60 | 27 | 0 | 4 | 63 | 41 | 100 | 3.54e-20 | 82.0 |
| MsG0480023448.01.T01 | MTR_7g114680 | 55.000 | 60 | 27 | 0 | 4 | 63 | 55 | 114 | 3.91e-20 | 82.0 |
| MsG0480023448.01.T01 | MTR_5g075220 | 56.667 | 60 | 26 | 0 | 4 | 63 | 58 | 117 | 5.04e-19 | 79.0 |
| MsG0480023448.01.T01 | MTR_1g103850 | 51.667 | 60 | 29 | 0 | 4 | 63 | 55 | 114 | 1.48e-18 | 77.4 |
| MsG0480023448.01.T01 | MTR_6g016260 | 55.000 | 60 | 27 | 0 | 4 | 63 | 37 | 96 | 2.17e-18 | 77.0 |
| MsG0480023448.01.T01 | MTR_4g028830 | 55.000 | 60 | 27 | 0 | 4 | 63 | 55 | 114 | 5.24e-18 | 75.9 |
| MsG0480023448.01.T01 | MTR_7g072860 | 55.000 | 60 | 27 | 0 | 4 | 63 | 59 | 118 | 1.32e-16 | 72.0 |
| MsG0480023448.01.T01 | MTR_7g072640 | 55.000 | 60 | 27 | 0 | 4 | 63 | 59 | 118 | 1.32e-16 | 72.0 |
| MsG0480023448.01.T01 | MTR_5g094390 | 43.333 | 60 | 34 | 0 | 4 | 63 | 58 | 117 | 1.23e-12 | 60.5 |
| MsG0480023448.01.T01 | MTR_5g094390 | 43.333 | 60 | 34 | 0 | 4 | 63 | 58 | 117 | 2.35e-12 | 58.9 |
| MsG0480023448.01.T01 | MTR_7g029020 | 50.980 | 51 | 25 | 0 | 13 | 63 | 66 | 116 | 4.04e-12 | 58.9 |
| MsG0480023448.01.T01 | MTR_3g056025 | 41.667 | 60 | 35 | 0 | 4 | 63 | 57 | 116 | 3.14e-11 | 56.6 |
| MsG0480023448.01.T01 | MTR_3g056010 | 41.667 | 60 | 35 | 0 | 4 | 63 | 57 | 116 | 4.28e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023448.01.T01 | AT1G49160 | 75.000 | 60 | 15 | 0 | 4 | 63 | 106 | 165 | 5.80e-27 | 101 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.38e-27 | 101 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.38e-27 | 101 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.38e-27 | 101 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.88e-27 | 100 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 59 | 117 | 7.88e-27 | 100 |
| MsG0480023448.01.T01 | AT3G18750 | 77.966 | 59 | 13 | 0 | 4 | 62 | 41 | 99 | 8.26e-27 | 100 |
| MsG0480023448.01.T01 | AT1G49160 | 75.000 | 60 | 15 | 0 | 4 | 63 | 106 | 165 | 3.49e-26 | 99.4 |
| MsG0480023448.01.T01 | AT1G49160 | 75.000 | 60 | 15 | 0 | 4 | 63 | 59 | 118 | 3.54e-26 | 99.4 |
| MsG0480023448.01.T01 | AT1G49160 | 75.000 | 60 | 15 | 0 | 4 | 63 | 41 | 100 | 3.73e-26 | 99.0 |
| MsG0480023448.01.T01 | AT5G41990 | 62.712 | 59 | 22 | 0 | 4 | 62 | 60 | 118 | 7.24e-23 | 89.7 |
| MsG0480023448.01.T01 | AT1G64630 | 63.793 | 58 | 21 | 0 | 4 | 61 | 47 | 104 | 3.30e-22 | 87.8 |
| MsG0480023448.01.T01 | AT3G04910 | 58.333 | 60 | 25 | 0 | 4 | 63 | 55 | 114 | 8.04e-21 | 84.0 |
| MsG0480023448.01.T01 | AT3G04910 | 58.333 | 60 | 25 | 0 | 4 | 63 | 32 | 91 | 8.11e-21 | 84.0 |
| MsG0480023448.01.T01 | AT3G48260 | 58.333 | 60 | 25 | 0 | 4 | 63 | 53 | 112 | 7.37e-20 | 81.3 |
| MsG0480023448.01.T01 | AT3G51630 | 60.000 | 60 | 24 | 0 | 4 | 63 | 56 | 115 | 2.55e-19 | 79.7 |
| MsG0480023448.01.T01 | AT3G51630 | 60.000 | 60 | 24 | 0 | 4 | 63 | 56 | 115 | 2.55e-19 | 79.7 |
| MsG0480023448.01.T01 | AT5G28080 | 53.333 | 60 | 28 | 0 | 4 | 63 | 56 | 115 | 5.04e-19 | 79.0 |
| MsG0480023448.01.T01 | AT5G58350 | 61.667 | 60 | 23 | 0 | 4 | 63 | 50 | 109 | 6.53e-19 | 78.6 |
| MsG0480023448.01.T01 | AT5G28080 | 54.717 | 53 | 24 | 0 | 11 | 63 | 26 | 78 | 2.52e-17 | 73.9 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 126 | 185 | 2.09e-15 | 68.6 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 126 | 185 | 2.09e-15 | 68.6 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 55 | 114 | 2.17e-15 | 68.6 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 55 | 114 | 2.21e-15 | 68.6 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 55 | 114 | 2.32e-15 | 68.6 |
| MsG0480023448.01.T01 | AT3G22420 | 45.000 | 60 | 33 | 0 | 4 | 63 | 55 | 114 | 2.46e-15 | 68.6 |
| MsG0480023448.01.T01 | AT5G55560 | 47.059 | 51 | 27 | 0 | 13 | 63 | 71 | 121 | 1.43e-11 | 57.8 |
Find 10 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTAAGATTCTATAATTCT+TGG | 0.277146 | 4:+85237350 | MsG0480023448.01.T01:CDS |
| ATTACTGAGTTGTTTACTTC+GGG | 0.369464 | 4:+85237403 | MsG0480023448.01.T01:CDS |
| AGTCACCAGGTGACTTAGAT+AGG | 0.421005 | 4:+85237281 | MsG0480023448.01.T01:CDS |
| GATTACTGAGTTGTTTACTT+CGG | 0.494689 | 4:+85237402 | MsG0480023448.01.T01:CDS |
| GATATTGGACTTACTGCTTG+AGG | 0.561458 | 4:-85237429 | None:intergenic |
| CTTGGATTGATGACAAGCGC+AGG | 0.580635 | 4:+85237368 | MsG0480023448.01.T01:CDS |
| GACGAGGTGTTACAGTCACC+AGG | 0.613888 | 4:+85237268 | MsG0480023448.01.T01:CDS |
| TATAGCCTATCTAAGTCACC+TGG | 0.644560 | 4:-85237286 | None:intergenic |
| TGCATCTCTTAAAATCGCTG+AGG | 0.662060 | 4:+85237317 | MsG0480023448.01.T01:CDS |
| GGGGACAGTTCAGATTGACG+AGG | 0.761538 | 4:+85237252 | MsG0480023448.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGTAAGATTCTATAATTCT+TGG | + | Chr4:85237350-85237369 | MsG0480023448.01.T01:CDS | 20.0% |
| ATTACTGAGTTGTTTACTTC+GGG | + | Chr4:85237403-85237422 | MsG0480023448.01.T01:CDS | 30.0% | |
| GATTACTGAGTTGTTTACTT+CGG | + | Chr4:85237402-85237421 | MsG0480023448.01.T01:CDS | 30.0% | |
| TATAGCCTATCTAAGTCACC+TGG | - | Chr4:85237289-85237308 | None:intergenic | 40.0% | |
| TGCATCTCTTAAAATCGCTG+AGG | + | Chr4:85237317-85237336 | MsG0480023448.01.T01:CDS | 40.0% | |
| AGTCACCAGGTGACTTAGAT+AGG | + | Chr4:85237281-85237300 | MsG0480023448.01.T01:CDS | 45.0% | |
| ! | CTTGGATTGATGACAAGCGC+AGG | + | Chr4:85237368-85237387 | MsG0480023448.01.T01:CDS | 50.0% |
| GGGGACAGTTCAGATTGACG+AGG | + | Chr4:85237252-85237271 | MsG0480023448.01.T01:CDS | 55.0% | |
| ! | GACGAGGTGTTACAGTCACC+AGG | + | Chr4:85237268-85237287 | MsG0480023448.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 85237250 | 85237441 | 85237250 | ID=MsG0480023448.01;Name=MsG0480023448.01 |
| Chr4 | mRNA | 85237250 | 85237441 | 85237250 | ID=MsG0480023448.01.T01;Parent=MsG0480023448.01;Name=MsG0480023448.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|63 |
| Chr4 | exon | 85237250 | 85237441 | 85237250 | ID=MsG0480023448.01.T01:exon:1514;Parent=MsG0480023448.01.T01 |
| Chr4 | CDS | 85237250 | 85237441 | 85237250 | ID=MsG0480023448.01.T01:cds;Parent=MsG0480023448.01.T01 |
| Gene Sequence |
| Protein sequence |
