Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005987.01.T01 | XP_013470213.1 | 98.438 | 64 | 1 | 0 | 4 | 67 | 232 | 295 | 2.90E-36 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005987.01.T01 | Q9LFR7 | 61.017 | 59 | 21 | 1 | 8 | 66 | 237 | 293 | 1.43E-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005987.01.T01 | G7ZZC3 | 98.438 | 64 | 1 | 0 | 4 | 67 | 232 | 295 | 1.38e-36 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000197.01 | MsG0180005987.01 | 0.809994 | 1.436693e-50 | 8.560531e-48 |
| MsG0180000300.01 | MsG0180005987.01 | 0.806956 | 6.393144e-50 | 3.516542e-47 |
| MsG0180001566.01 | MsG0180005987.01 | 0.816356 | 5.768024e-52 | 4.078131e-49 |
| MsG0180002071.01 | MsG0180005987.01 | 0.809610 | 1.737992e-50 | 1.025181e-47 |
| MsG0180003361.01 | MsG0180005987.01 | 0.815240 | 1.023244e-51 | 7.016024e-49 |
| MsG0180003444.01 | MsG0180005987.01 | 0.814374 | 1.591598e-51 | 1.066117e-48 |
| MsG0180004060.01 | MsG0180005987.01 | 0.805574 | 1.249907e-49 | 6.635779e-47 |
| MsG0180004388.01 | MsG0180005987.01 | 0.807206 | 5.661171e-50 | 3.134226e-47 |
| MsG0180005987.01 | MsG0180006023.01 | 0.828855 | 7.128168e-55 | 7.170805e-52 |
| MsG0180005987.01 | MsG0180006235.01 | 0.807650 | 4.556973e-50 | 2.552121e-47 |
| MsG0180005987.01 | MsG0280006412.01 | 0.821588 | 3.727635e-53 | 3.045270e-50 |
| MsG0180005987.01 | MsG0280007414.01 | 0.803049 | 4.197130e-49 | 2.088114e-46 |
| MsG0180005987.01 | MsG0280007458.01 | 0.814987 | 1.164146e-51 | 7.927961e-49 |
| MsG0180005987.01 | MsG0280007609.01 | 0.840949 | 6.388851e-58 | 9.245899e-55 |
| MsG0180005987.01 | MsG0280007740.01 | 0.808267 | 3.368268e-50 | 1.917379e-47 |
| MsG0180005987.01 | MsG0280008280.01 | 0.806261 | 8.965782e-50 | 4.844681e-47 |
| MsG0180005987.01 | MsG0280008349.01 | 0.842350 | 2.728716e-58 | 4.126207e-55 |
| MsG0180005987.01 | MsG0280009514.01 | 0.817307 | 3.529637e-52 | 2.561156e-49 |
| MsG0180005987.01 | MsG0280010389.01 | 0.826637 | 2.432115e-54 | 2.293809e-51 |
| MsG0180005987.01 | MsG0280010551.01 | 0.827988 | 1.153879e-54 | 1.132034e-51 |
| MsG0180005987.01 | MsG0280010553.01 | 0.831198 | 1.912797e-55 | 2.060879e-52 |
| MsG0180005987.01 | MsG0280010614.01 | 0.813354 | 2.671166e-51 | 1.740432e-48 |
| MsG0180005987.01 | MsG0280011105.01 | 0.804805 | 1.810735e-49 | 9.425654e-47 |
| MsG0180005987.01 | MsG0280011258.01 | 0.810385 | 1.183649e-50 | 7.124425e-48 |
| MsG0180005987.01 | MsG0280011293.01 | 0.802495 | 5.460223e-49 | 2.678509e-46 |
| MsG0180005987.01 | MsG0380014118.01 | 0.811874 | 5.626714e-51 | 3.523334e-48 |
| MsG0180005987.01 | MsG0380014162.01 | -0.803581 | 3.255853e-49 | 1.642272e-46 |
| MsG0180005987.01 | MsG0380016765.01 | 0.820060 | 8.373814e-53 | 6.557205e-50 |
| MsG0180005987.01 | MsG0380016859.01 | 0.810041 | 1.403659e-50 | 8.373942e-48 |
| MsG0180005987.01 | MsG0380017177.01 | 0.823070 | 1.687771e-53 | 1.438308e-50 |
| MsG0180005987.01 | MsG0380017195.01 | 0.806385 | 8.438609e-50 | 4.573964e-47 |
| MsG0180005987.01 | MsG0380017232.01 | 0.822019 | 2.963712e-53 | 2.450924e-50 |
| MsG0180005987.01 | MsG0380017233.01 | 0.813580 | 2.382081e-51 | 1.561616e-48 |
| MsG0180005987.01 | MsG0380017266.01 | -0.804234 | 2.381843e-49 | 1.221610e-46 |
| MsG0180005987.01 | MsG0480018641.01 | 0.806264 | 8.950825e-50 | 4.836945e-47 |
| MsG0180005987.01 | MsG0480018780.01 | 0.802915 | 4.473483e-49 | 2.218127e-46 |
| MsG0180005987.01 | MsG0480018787.01 | 0.808316 | 3.288261e-50 | 1.874199e-47 |
| MsG0180005987.01 | MsG0480020242.01 | 0.831347 | 1.757871e-55 | 1.902435e-52 |
| MsG0180005987.01 | MsG0480020591.01 | 0.810063 | 1.388329e-50 | 8.287001e-48 |
| MsG0180005987.01 | MsG0480021350.01 | 0.832469 | 9.286814e-56 | 1.038938e-52 |
| MsG0180005987.01 | MsG0480022480.01 | 0.834841 | 2.374164e-56 | 2.851617e-53 |
| MsG0180005987.01 | MsG0480022597.01 | 0.836338 | 9.920254e-57 | 1.246553e-53 |
| MsG0180005987.01 | MsG0480022598.01 | 0.821997 | 2.998062e-53 | 2.477952e-50 |
| MsG0180005987.01 | MsG0480023421.01 | 0.803245 | 3.822438e-49 | 1.911325e-46 |
| MsG0180005987.01 | MsG0480023500.01 | 0.806154 | 9.442485e-50 | 5.088166e-47 |
| MsG0180005987.01 | MsG0480023718.01 | 0.816305 | 5.923528e-52 | 4.182325e-49 |
| MsG0180005987.01 | MsG0580024073.01 | 0.821894 | 3.167434e-53 | 2.610400e-50 |
| MsG0180005987.01 | MsG0580024477.01 | 0.811663 | 6.254762e-51 | 3.894554e-48 |
| MsG0180005987.01 | MsG0580024618.01 | 0.813218 | 2.860460e-51 | 1.856731e-48 |
| MsG0180005987.01 | MsG0580024733.01 | 0.821333 | 4.270262e-53 | 3.463989e-50 |
| MsG0180005987.01 | MsG0580029189.01 | 0.826657 | 2.405798e-54 | 2.270314e-51 |
| MsG0180005987.01 | MsG0580029198.01 | 0.803028 | 4.237728e-49 | 2.107231e-46 |
| MsG0180005987.01 | MsG0580029278.01 | 0.801817 | 7.529576e-49 | 3.630487e-46 |
| MsG0180005987.01 | MsG0580029960.01 | 0.800015 | 1.757231e-48 | 8.094622e-46 |
| MsG0180005987.01 | MsG0580030235.01 | 0.803007 | 4.280179e-49 | 2.127136e-46 |
| MsG0180005987.01 | MsG0680030685.01 | 0.816260 | 6.061353e-52 | 4.274284e-49 |
| MsG0180005987.01 | MsG0680031114.01 | 0.813273 | 2.782810e-51 | 1.809062e-48 |
| MsG0180005987.01 | MsG0780037975.01 | 0.827627 | 1.409621e-54 | 1.368143e-51 |
| MsG0180005987.01 | MsG0780038495.01 | 0.811617 | 6.400568e-51 | 3.980579e-48 |
| MsG0180005987.01 | MsG0780038937.01 | 0.812022 | 5.225145e-51 | 3.285008e-48 |
| MsG0180005987.01 | MsG0780040044.01 | 0.813888 | 2.037307e-51 | 1.346826e-48 |
| MsG0180005987.01 | MsG0780040488.01 | 0.829080 | 6.288473e-55 | 6.368337e-52 |
| MsG0180005987.01 | MsG0780040712.01 | 0.804273 | 2.337625e-49 | 1.200187e-46 |
| MsG0180005987.01 | MsG0780040947.01 | 0.816743 | 4.725744e-52 | 3.376579e-49 |
| MsG0180005987.01 | MsG0780041376.01 | 0.800672 | 1.291425e-48 | 6.049052e-46 |
| MsG0180005987.01 | MsG0780041787.01 | 0.803905 | 2.788901e-49 | 1.418484e-46 |
| MsG0180005987.01 | MsG0880042698.01 | 0.816566 | 5.175523e-52 | 3.680181e-49 |
| MsG0180005987.01 | MsG0880045583.01 | 0.807162 | 5.782664e-50 | 3.197828e-47 |
| MsG0180005987.01 | MsG0880046762.01 | 0.822533 | 2.251385e-53 | 1.889621e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005987.01.T01 | MTR_1g111280 | 98.438 | 64 | 1 | 0 | 4 | 67 | 232 | 295 | 3.51e-40 | 134 |
| MsG0180005987.01.T01 | MTR_7g406880 | 54.412 | 68 | 27 | 3 | 4 | 67 | 235 | 302 | 1.03e-18 | 77.8 |
| MsG0180005987.01.T01 | MTR_1g089310 | 49.180 | 61 | 29 | 2 | 10 | 68 | 254 | 314 | 9.96e-12 | 58.5 |
| MsG0180005987.01.T01 | MTR_1g089310 | 49.180 | 61 | 29 | 2 | 10 | 68 | 238 | 298 | 1.13e-11 | 58.2 |
| MsG0180005987.01.T01 | MTR_4g050140 | 46.774 | 62 | 27 | 2 | 10 | 67 | 212 | 271 | 1.32e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005987.01.T01 | AT5G16080 | 61.017 | 59 | 21 | 1 | 8 | 66 | 237 | 293 | 1.46e-18 | 77.4 |
| MsG0180005987.01.T01 | AT1G68620 | 45.312 | 64 | 34 | 1 | 3 | 66 | 222 | 284 | 5.18e-13 | 62.0 |
Find 18 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCAGCTTCAGATACTTAT+TGG | 0.192800 | 1:-98509108 | MsG0180005987.01.T01:CDS |
| GTGTAATCCATTGGTGAAAA+TGG | 0.278139 | 1:-98509044 | MsG0180005987.01.T01:CDS |
| CTTGGAGTTTGTGATGCTTT+AGG | 0.300976 | 1:-98508951 | None:intergenic |
| GAAATGCATGGACAATTATC+TGG | 0.379449 | 1:-98509145 | MsG0180005987.01.T01:CDS |
| TGGCGATTGGCATTACCATA+TGG | 0.434796 | 1:-98509088 | MsG0180005987.01.T01:CDS |
| TTCAGATACTTATTGGCGAT+TGG | 0.468433 | 1:-98509101 | MsG0180005987.01.T01:CDS |
| TGATCACGATCTTCACCATA+TGG | 0.493029 | 1:+98509073 | None:intergenic |
| ATCTGAGATGGATATATTGA+AGG | 0.500971 | 1:-98508981 | MsG0180005987.01.T01:CDS |
| TATATTGAAGGATAGGAACT+TGG | 0.516422 | 1:-98508969 | MsG0180005987.01.T01:CDS |
| AGATGGATATATTGAAGGAT+AGG | 0.521304 | 1:-98508976 | MsG0180005987.01.T01:CDS |
| TCAGATATACACACCAACAT+AGG | 0.575792 | 1:+98508998 | None:intergenic |
| GCTACTGATGATGCCTATGT+TGG | 0.579488 | 1:-98509011 | MsG0180005987.01.T01:CDS |
| AACATTATCAGAGAAATGCA+TGG | 0.593132 | 1:-98509157 | None:intergenic |
| TTCACCAATGGATTACACCA+TGG | 0.598695 | 1:+98509049 | None:intergenic |
| GTTGGTGTGTATATCTGAGA+TGG | 0.604384 | 1:-98508993 | MsG0180005987.01.T01:CDS |
| TCATCCATGGTGTAATCCAT+TGG | 0.611826 | 1:-98509053 | MsG0180005987.01.T01:CDS |
| TAATCCATTGGTGAAAATGG+AGG | 0.623717 | 1:-98509041 | MsG0180005987.01.T01:CDS |
| GTGAAGATCGTGATCATCCA+TGG | 0.631742 | 1:-98509066 | MsG0180005987.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGATATATTGAAGGAT+AGG | - | Chr1:98509118-98509137 | MsG0180005987.01.T01:CDS | 30.0% | |
| TATATTGAAGGATAGGAACT+TGG | - | Chr1:98509125-98509144 | MsG0180005987.01.T01:CDS | 30.0% | |
| ! | ATCTGAGATGGATATATTGA+AGG | - | Chr1:98509113-98509132 | MsG0180005987.01.T01:CDS | 30.0% |
| GTGTAATCCATTGGTGAAAA+TGG | - | Chr1:98509050-98509069 | MsG0180005987.01.T01:CDS | 35.0% | |
| TAATCCATTGGTGAAAATGG+AGG | - | Chr1:98509053-98509072 | MsG0180005987.01.T01:CDS | 35.0% | |
| TAGCAGCTTCAGATACTTAT+TGG | - | Chr1:98508986-98509005 | MsG0180005987.01.T01:CDS | 35.0% | |
| TCAGATATACACACCAACAT+AGG | + | Chr1:98509099-98509118 | None:intergenic | 35.0% | |
| TTCAGATACTTATTGGCGAT+TGG | - | Chr1:98508993-98509012 | MsG0180005987.01.T01:CDS | 35.0% | |
| ! | AATTCCTCCATTTTCACCAA+TGG | + | Chr1:98509060-98509079 | None:intergenic | 35.0% |
| GTTGGTGTGTATATCTGAGA+TGG | - | Chr1:98509101-98509120 | MsG0180005987.01.T01:CDS | 40.0% | |
| TCATCCATGGTGTAATCCAT+TGG | - | Chr1:98509041-98509060 | MsG0180005987.01.T01:CDS | 40.0% | |
| TGATCACGATCTTCACCATA+TGG | + | Chr1:98509024-98509043 | None:intergenic | 40.0% | |
| !! | TTCACCAATGGATTACACCA+TGG | + | Chr1:98509048-98509067 | None:intergenic | 40.0% |
| GTGAAGATCGTGATCATCCA+TGG | - | Chr1:98509028-98509047 | MsG0180005987.01.T01:CDS | 45.0% | |
| TGGCGATTGGCATTACCATA+TGG | - | Chr1:98509006-98509025 | MsG0180005987.01.T01:CDS | 45.0% | |
| ! | GCTACTGATGATGCCTATGT+TGG | - | Chr1:98509083-98509102 | MsG0180005987.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 98508952 | 98509164 | 98508952 | ID=MsG0180005987.01;Name=MsG0180005987.01 |
| Chr1 | mRNA | 98508952 | 98509164 | 98508952 | ID=MsG0180005987.01.T01;Parent=MsG0180005987.01;Name=MsG0180005987.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|70 |
| Chr1 | exon | 98508952 | 98509164 | 98508952 | ID=MsG0180005987.01.T01:exon:16514;Parent=MsG0180005987.01.T01 |
| Chr1 | CDS | 98508952 | 98509164 | 98508952 | ID=MsG0180005987.01.T01:cds;Parent=MsG0180005987.01.T01 |
| Gene Sequence |
| Protein sequence |
