AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040947.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040947.01.T01 MTR_7g101730 98.673 226 3 0 1 226 263 488 1.01e-158 452
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040947.01.T01 AT2G37035 53.982 226 69 1 3 228 243 433 2.35e-78 242

Find 49 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATATTCTCTGTGGATTTC+TGG 0.061482 7:+84671804 MsG0780040947.01.T01:CDS
GTTATGTCGCCTTGGGTTAC+TGG 0.193809 7:+84671719 MsG0780040947.01.T01:CDS
TTTGGTTACATGGTTTCTTA+TGG 0.235123 7:+84671766 MsG0780040947.01.T01:CDS
GTTACATATTCGTGTGTATT+AGG 0.289371 7:+84671464 MsG0780040947.01.T01:CDS
GTTCAACTACTGGTGTGGTT+TGG 0.305022 7:+84671531 MsG0780040947.01.T01:CDS
CAAGGCGACATAACCGAAAA+TGG 0.306788 7:-84671710 None:intergenic
TTCAACTACTGGTGTGGTTT+GGG 0.319340 7:+84671532 MsG0780040947.01.T01:CDS
TGTGGATTTCTGGGTTGCTT+TGG 0.322485 7:+84671814 MsG0780040947.01.T01:CDS
AATCAACAGGGTAGGAGGTT+TGG 0.323638 7:+84672076 MsG0780040947.01.T01:CDS
TATATTCTCTGTGGATTTCT+GGG 0.335772 7:+84671805 MsG0780040947.01.T01:CDS
TACCATGGAGGTTTACTTCA+TGG 0.347990 7:+84672009 MsG0780040947.01.T01:CDS
TGACACAGCTTGTGGGAATA+TGG 0.355268 7:+84671624 MsG0780040947.01.T01:CDS
ATCAACAGGGTAGGAGGTTT+GGG 0.356159 7:+84672077 MsG0780040947.01.T01:CDS
TTTGATAAGGTGGGCTGTTA+GGG 0.367104 7:+84671595 MsG0780040947.01.T01:CDS
TCAGTTGTTAATGATCTTGT+AGG 0.382451 7:+84671500 MsG0780040947.01.T01:CDS
GGATTTCTGGGTTGCTTTGG+AGG 0.384827 7:+84671817 MsG0780040947.01.T01:CDS
GAGTAGGGATGCCAATCAAC+AGG 0.400674 7:+84672063 MsG0780040947.01.T01:CDS
CGTTCTTTAAGCTATTTGTT+CGG 0.433372 7:+84671675 MsG0780040947.01.T01:CDS
TGCTTGGAAGCTGTGCTTTG+TGG 0.441214 7:+84671923 MsG0780040947.01.T01:CDS
AGGTTTACTTCATGGTTGCT+TGG 0.460321 7:+84672017 MsG0780040947.01.T01:CDS
GTTTGGGCAGAGGGAAATTG+AGG 0.473811 7:+84672093 MsG0780040947.01.T01:CDS
GGGTAGGAGGTTTGGGCAGA+GGG 0.480015 7:+84672084 MsG0780040947.01.T01:CDS
AGGGTAGGAGGTTTGGGCAG+AGG 0.486226 7:+84672083 MsG0780040947.01.T01:CDS
AGTAAGGAGATTGTGAAGGA+AGG 0.489890 7:+84671854 MsG0780040947.01.T01:CDS
AAACCTCCTACCCTGTTGAT+TGG 0.504079 7:-84672074 None:intergenic
GGAAGGTTTAGTTCAACTAC+TGG 0.504708 7:+84671521 MsG0780040947.01.T01:CDS
CATGGTAGACTGAAACATCT+TGG 0.507976 7:-84671993 None:intergenic
TGCTTTGTGGATCGTTAATG+AGG 0.519695 7:+84671936 MsG0780040947.01.T01:CDS
GTAAGGAGATTGTGAAGGAA+GGG 0.524150 7:+84671855 MsG0780040947.01.T01:CDS
GAAATTGAGGGCTTAATCGA+TGG 0.529867 7:+84672106 MsG0780040947.01.T01:CDS
AGGTAGTAAGGAGATTGTGA+AGG 0.545123 7:+84671850 MsG0780040947.01.T01:CDS
TTTGGGCAGAGGGAAATTGA+GGG 0.547206 7:+84672094 MsG0780040947.01.T01:CDS
ACTACTGGTGTGGTTTGGGA+TGG 0.550541 7:+84671536 MsG0780040947.01.T01:CDS
CAGAAAAGCCTGATAAAACA+TGG 0.551031 7:-84671894 None:intergenic
GGATGCAGTGACACAGCTTG+TGG 0.553261 7:+84671616 MsG0780040947.01.T01:CDS
GATGCAGTGACACAGCTTGT+GGG 0.555205 7:+84671617 MsG0780040947.01.T01:CDS
GTTTAGTTCAACTACTGGTG+TGG 0.574767 7:+84671526 MsG0780040947.01.T01:CDS
ATGCCAATCAACAGGGTAGG+AGG 0.575686 7:+84672071 MsG0780040947.01.T01:CDS
TTGTTAATGATCTTGTAGGA+AGG 0.591111 7:+84671504 MsG0780040947.01.T01:CDS
AAATTGAGGGCTTAATCGAT+GGG 0.595021 7:+84672107 MsG0780040947.01.T01:CDS
AACCATGAAGTAAACCTCCA+TGG 0.610271 7:-84672011 None:intergenic
TTCTCAAAACCAGTAACCCA+AGG 0.613465 7:-84671728 None:intergenic
GGGATGCCAATCAACAGGGT+AGG 0.627186 7:+84672068 MsG0780040947.01.T01:CDS
TTTGTGGATCGTTAATGAGG+TGG 0.632821 7:+84671939 MsG0780040947.01.T01:CDS
CAAGATGTTTCAGTCTACCA+TGG 0.640369 7:+84671994 MsG0780040947.01.T01:CDS
TGTGACGTTTATATTCTCTG+TGG 0.646145 7:+84671796 MsG0780040947.01.T01:CDS
AGGTTTGAAGAGGATTTCTG+CGG 0.648624 7:+84671568 MsG0780040947.01.T01:CDS
AGTAGGGATGCCAATCAACA+GGG 0.665230 7:+84672064 MsG0780040947.01.T01:CDS
GATGTTTCAGTCTACCATGG+AGG 0.685865 7:+84671997 MsG0780040947.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CGTTCTTTAAGCTATTTGTT+CGG + Chr7:84671675-84671694 MsG0780040947.01.T01:CDS 30.0%
GTTACATATTCGTGTGTATT+AGG + Chr7:84671464-84671483 MsG0780040947.01.T01:CDS 30.0%
TATATTCTCTGTGGATTTCT+GGG + Chr7:84671805-84671824 MsG0780040947.01.T01:CDS 30.0%
TCAGTTGTTAATGATCTTGT+AGG + Chr7:84671500-84671519 MsG0780040947.01.T01:CDS 30.0%
TTATATTCTCTGTGGATTTC+TGG + Chr7:84671804-84671823 MsG0780040947.01.T01:CDS 30.0%
TTGTTAATGATCTTGTAGGA+AGG + Chr7:84671504-84671523 MsG0780040947.01.T01:CDS 30.0%
TTTGGTTACATGGTTTCTTA+TGG + Chr7:84671766-84671785 MsG0780040947.01.T01:CDS 30.0%
! ATGGTATTTTGCTGAAATTG+AGG + Chr7:84671643-84671662 MsG0780040947.01.T01:CDS 30.0%
! GTTTTCAATTAGGTTTGAAG+AGG + Chr7:84671558-84671577 MsG0780040947.01.T01:CDS 30.0%
!! TTATTTTGTGGCTAAGAGTA+GGG + Chr7:84672048-84672067 MsG0780040947.01.T01:CDS 30.0%
!!! GTTGAAATTGATGCCATTTT+CGG + Chr7:84671697-84671716 MsG0780040947.01.T01:CDS 30.0%
!!! TTGGCTTGTGTTTTATTTTG+TGG + Chr7:84672036-84672055 MsG0780040947.01.T01:CDS 30.0%
!!! TTTATTTTGTGGCTAAGAGT+AGG + Chr7:84672047-84672066 MsG0780040947.01.T01:CDS 30.0%
AAATTGAGGGCTTAATCGAT+GGG + Chr7:84672107-84672126 MsG0780040947.01.T01:CDS 35.0%
CAGAAAAGCCTGATAAAACA+TGG - Chr7:84671897-84671916 None:intergenic 35.0%
TGTGACGTTTATATTCTCTG+TGG + Chr7:84671796-84671815 MsG0780040947.01.T01:CDS 35.0%
! ATCGTTAATGAGGTGGTTTT+TGG + Chr7:84671946-84671965 MsG0780040947.01.T01:CDS 35.0%
! GGCTTTTCTGATTAAATGCT+TGG + Chr7:84671907-84671926 MsG0780040947.01.T01:CDS 35.0%
! GTTGTCTTCCATGTTTTATC+AGG + Chr7:84671886-84671905 MsG0780040947.01.T01:CDS 35.0%
!! GTTTGGGATGGTTTTCAATT+AGG + Chr7:84671548-84671567 MsG0780040947.01.T01:CDS 35.0%
!!! GTTTTGAGAAGGAGATTTCT+GGG + Chr7:84671741-84671760 MsG0780040947.01.T01:CDS 35.0%
!!! TTTCTGCGGTTGTTTTGATA+AGG + Chr7:84671582-84671601 MsG0780040947.01.T01:CDS 35.0%
!!! TTTCTGGGTTTTTGGTTACA+TGG + Chr7:84671756-84671775 MsG0780040947.01.T01:CDS 35.0%
AACCATGAAGTAAACCTCCA+TGG - Chr7:84672014-84672033 None:intergenic 40.0%
AGGTAGTAAGGAGATTGTGA+AGG + Chr7:84671850-84671869 MsG0780040947.01.T01:CDS 40.0%
AGGTTTACTTCATGGTTGCT+TGG + Chr7:84672017-84672036 MsG0780040947.01.T01:CDS 40.0%
AGGTTTGAAGAGGATTTCTG+CGG + Chr7:84671568-84671587 MsG0780040947.01.T01:CDS 40.0%
AGTAAGGAGATTGTGAAGGA+AGG + Chr7:84671854-84671873 MsG0780040947.01.T01:CDS 40.0%
CAAGATGTTTCAGTCTACCA+TGG + Chr7:84671994-84672013 MsG0780040947.01.T01:CDS 40.0%
CATGGTAGACTGAAACATCT+TGG - Chr7:84671996-84672015 None:intergenic 40.0%
GAAATTGAGGGCTTAATCGA+TGG + Chr7:84672106-84672125 MsG0780040947.01.T01:CDS 40.0%
GTAAGGAGATTGTGAAGGAA+GGG + Chr7:84671855-84671874 MsG0780040947.01.T01:CDS 40.0%
TACCATGGAGGTTTACTTCA+TGG + Chr7:84672009-84672028 MsG0780040947.01.T01:CDS 40.0%
TGCTTTGTGGATCGTTAATG+AGG + Chr7:84671936-84671955 MsG0780040947.01.T01:CDS 40.0%
TTCAACTACTGGTGTGGTTT+GGG + Chr7:84671532-84671551 MsG0780040947.01.T01:CDS 40.0%
TTCTCAAAACCAGTAACCCA+AGG - Chr7:84671731-84671750 None:intergenic 40.0%
TTTGTGGATCGTTAATGAGG+TGG + Chr7:84671939-84671958 MsG0780040947.01.T01:CDS 40.0%
! ATTTTCGGTTATGTCGCCTT+GGG + Chr7:84671712-84671731 MsG0780040947.01.T01:CDS 40.0%
! GGAAGGTTTAGTTCAACTAC+TGG + Chr7:84671521-84671540 MsG0780040947.01.T01:CDS 40.0%
! GTTTAGTTCAACTACTGGTG+TGG + Chr7:84671526-84671545 MsG0780040947.01.T01:CDS 40.0%
!! GAAGGAGATTTCTGGGTTTT+TGG + Chr7:84671748-84671767 MsG0780040947.01.T01:CDS 40.0%
!! GGATTTTAGGAGAGGTAGTA+AGG + Chr7:84671838-84671857 MsG0780040947.01.T01:CDS 40.0%
!! TTTGATAAGGTGGGCTGTTA+GGG + Chr7:84671595-84671614 MsG0780040947.01.T01:CDS 40.0%
!!! GGTTTTGAGAAGGAGATTTC+TGG + Chr7:84671740-84671759 MsG0780040947.01.T01:CDS 40.0%
!!! TGCGGTTGTTTTGATAAGGT+GGG + Chr7:84671586-84671605 MsG0780040947.01.T01:CDS 40.0%
!!! TGGTTTTTGGCTCGAATTTG+CGG + Chr7:84671959-84671978 MsG0780040947.01.T01:CDS 40.0%
!!! TTGGGTTACTGGTTTTGAGA+AGG + Chr7:84671730-84671749 MsG0780040947.01.T01:CDS 40.0%
!!! TTTTGATAAGGTGGGCTGTT+AGG + Chr7:84671594-84671613 MsG0780040947.01.T01:CDS 40.0%
AAACCTCCTACCCTGTTGAT+TGG - Chr7:84672077-84672096 None:intergenic 45.0%
AATCAACAGGGTAGGAGGTT+TGG + Chr7:84672076-84672095 MsG0780040947.01.T01:CDS 45.0%
AGTAGGGATGCCAATCAACA+GGG + Chr7:84672064-84672083 MsG0780040947.01.T01:CDS 45.0%
ATCAACAGGGTAGGAGGTTT+GGG + Chr7:84672077-84672096 MsG0780040947.01.T01:CDS 45.0%
CAAGGCGACATAACCGAAAA+TGG - Chr7:84671713-84671732 None:intergenic 45.0%
GATGTTTCAGTCTACCATGG+AGG + Chr7:84671997-84672016 MsG0780040947.01.T01:CDS 45.0%
GTTCAACTACTGGTGTGGTT+TGG + Chr7:84671531-84671550 MsG0780040947.01.T01:CDS 45.0%
TGACACAGCTTGTGGGAATA+TGG + Chr7:84671624-84671643 MsG0780040947.01.T01:CDS 45.0%
TTTGGGCAGAGGGAAATTGA+GGG + Chr7:84672094-84672113 MsG0780040947.01.T01:CDS 45.0%
! CATTTTCGGTTATGTCGCCT+TGG + Chr7:84671711-84671730 MsG0780040947.01.T01:CDS 45.0%
! TGTGGATTTCTGGGTTGCTT+TGG + Chr7:84671814-84671833 MsG0780040947.01.T01:CDS 45.0%
!!! CTGCGGTTGTTTTGATAAGG+TGG + Chr7:84671585-84671604 MsG0780040947.01.T01:CDS 45.0%
!!! GCTTTGGAGGATTTTAGGAG+AGG + Chr7:84671830-84671849 MsG0780040947.01.T01:CDS 45.0%
!!! GGGTTGCTTTGGAGGATTTT+AGG + Chr7:84671825-84671844 MsG0780040947.01.T01:CDS 45.0%
!!! GGTTTTTGGCTCGAATTTGC+GGG + Chr7:84671960-84671979 MsG0780040947.01.T01:CDS 45.0%
ATGCCAATCAACAGGGTAGG+AGG + Chr7:84672071-84672090 MsG0780040947.01.T01:CDS 50.0%
GAGTAGGGATGCCAATCAAC+AGG + Chr7:84672063-84672082 MsG0780040947.01.T01:CDS 50.0%
GATGCAGTGACACAGCTTGT+GGG + Chr7:84671617-84671636 MsG0780040947.01.T01:CDS 50.0%
GTTATGTCGCCTTGGGTTAC+TGG + Chr7:84671719-84671738 MsG0780040947.01.T01:CDS 50.0%
GTTTGGGCAGAGGGAAATTG+AGG + Chr7:84672093-84672112 MsG0780040947.01.T01:CDS 50.0%
TGCTTGGAAGCTGTGCTTTG+TGG + Chr7:84671923-84671942 MsG0780040947.01.T01:CDS 50.0%
! ACTACTGGTGTGGTTTGGGA+TGG + Chr7:84671536-84671555 MsG0780040947.01.T01:CDS 50.0%
! GGATTTCTGGGTTGCTTTGG+AGG + Chr7:84671817-84671836 MsG0780040947.01.T01:CDS 50.0%
GGATGCAGTGACACAGCTTG+TGG + Chr7:84671616-84671635 MsG0780040947.01.T01:CDS 55.0%
GGGATGCCAATCAACAGGGT+AGG + Chr7:84672068-84672087 MsG0780040947.01.T01:CDS 55.0%
AGGGTAGGAGGTTTGGGCAG+AGG + Chr7:84672083-84672102 MsG0780040947.01.T01:CDS 60.0%
GGGTAGGAGGTTTGGGCAGA+GGG + Chr7:84672084-84672103 MsG0780040947.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 84671452 84672138 84671452 ID=MsG0780040947.01;Name=MsG0780040947.01
Chr7 mRNA 84671452 84672138 84671452 ID=MsG0780040947.01.T01;Parent=MsG0780040947.01;Name=MsG0780040947.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|228
Chr7 exon 84671452 84672138 84671452 ID=MsG0780040947.01.T01:exon:18714;Parent=MsG0780040947.01.T01
Chr7 CDS 84671452 84672138 84671452 ID=MsG0780040947.01.T01:cds;Parent=MsG0780040947.01.T01
Gene Sequence

>MsG0780040947.01.T01

ATGGTTTTTCTTGTTACATATTCGTGTGTATTAGGTGTTGTTGTTGTTTCAGTTGTTAATGATCTTGTAGGAAGGTTTAGTTCAACTACTGGTGTGGTTTGGGATGGTTTTCAATTAGGTTTGAAGAGGATTTCTGCGGTTGTTTTGATAAGGTGGGCTGTTAGGGATGCAGTGACACAGCTTGTGGGAATATGGTATTTTGCTGAAATTGAGGATCAATTTTCGTTCTTTAAGCTATTTGTTCGGTTGAAATTGATGCCATTTTCGGTTATGTCGCCTTGGGTTACTGGTTTTGAGAAGGAGATTTCTGGGTTTTTGGTTACATGGTTTCTTATGGATACATTTGTGACGTTTATATTCTCTGTGGATTTCTGGGTTGCTTTGGAGGATTTTAGGAGAGGTAGTAAGGAGATTGTGAAGGAAGGGTGTTATTTGTTGTCTTCCATGTTTTATCAGGCTTTTCTGATTAAATGCTTGGAAGCTGTGCTTTGTGGATCGTTAATGAGGTGGTTTTTGGCTCGAATTTGCGGGAGAGTTTTTGCCAAGATGTTTCAGTCTACCATGGAGGTTTACTTCATGGTTGCTTGGCTTGTGTTTTATTTTGTGGCTAAGAGTAGGGATGCCAATCAACAGGGTAGGAGGTTTGGGCAGAGGGAAATTGAGGGCTTAATCGATGGGCACAGATGA

Protein sequence

>MsG0780040947.01.T01

MVFLVTYSCVLGVVVVSVVNDLVGRFSSTTGVVWDGFQLGLKRISAVVLIRWAVRDAVTQLVGIWYFAEIEDQFSFFKLFVRLKLMPFSVMSPWVTGFEKEISGFLVTWFLMDTFVTFIFSVDFWVALEDFRRGSKEIVKEGCYLLSSMFYQAFLIKCLEAVLCGSLMRWFLARICGRVFAKMFQSTMEVYFMVAWLVFYFVAKSRDANQQGRRFGQREIEGLIDGHR*