AlfalfaGEDB | MsG0280010177.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010177.01

KEGG: K03327
GO: GO:0006855 GO:0015238 GO:0015297 GO:0016020 GO:0055085
PFAM: PF01554

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010177.01.T01	XP_013464672.1	67.327	202	38	4	65	240	220	419	8.45E-71	235
MsG0280010177.01.T01	XP_013464672.1	74.576	59	15	0	1	59	31	89	5.79E-15	85.9

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010177.01.T01	Q9LUH2	46.073	191	74	3	68	233	218	404	1.17E-38	143

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010177.01.T01	A0A072VA71	67.327	202	38	4	65	240	220	419	4.04e-71	235

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000982.01	MsG0280010177.01	0.820396	7.012562e-53	5.542565e-50
MsG0180001886.01	MsG0280010177.01	0.808662	2.773605e-50	1.595130e-47
MsG0180003548.01	MsG0280010177.01	0.804211	2.408220e-49	1.234442e-46
MsG0180003572.01	MsG0280010177.01	0.841165	5.607672e-58	8.169468e-55
MsG0180004050.01	MsG0280010177.01	0.805091	1.578304e-49	8.275910e-47
MsG0180005993.01	MsG0280010177.01	0.815276	1.004301e-51	6.893471e-49
MsG0280006422.01	MsG0280010177.01	0.830578	2.713310e-55	2.870307e-52
MsG0280006483.01	MsG0280010177.01	0.814346	1.614711e-51	1.080767e-48
MsG0280008936.01	MsG0280010177.01	0.819317	1.237285e-52	9.488459e-50
MsG0280009739.01	MsG0280010177.01	0.807955	3.924326e-50	2.215503e-47
MsG0280010176.01	MsG0280010177.01	0.804436	2.161866e-49	1.114591e-46
MsG0280010177.01	MsG0280011156.01	0.808435	3.101927e-50	1.773520e-47
MsG0280010177.01	MsG0280011388.01	0.811842	5.719064e-51	3.578106e-48
MsG0280010177.01	MsG0380014647.01	0.814101	1.828623e-51	1.215797e-48
MsG0280010177.01	MsG0380015701.01	0.838718	2.434080e-57	3.288378e-54
MsG0280010177.01	MsG0380016067.01	0.802980	4.336905e-49	2.153866e-46
MsG0280010177.01	MsG0380016860.01	0.818621	1.781921e-52	1.340510e-49
MsG0280010177.01	MsG0380017039.01	0.821704	3.504516e-53	2.872632e-50
MsG0280010177.01	MsG0380017351.01	0.821661	3.585718e-53	2.935505e-50
MsG0280010177.01	MsG0480018894.01	0.800158	1.643596e-48	7.598434e-46
MsG0280010177.01	MsG0480020560.01	0.806906	6.550346e-50	3.598390e-47
MsG0280010177.01	MsG0480021448.01	0.840617	7.807631e-58	1.118267e-54
MsG0280010177.01	MsG0480021453.01	0.835473	1.643952e-56	2.012348e-53
MsG0280010177.01	MsG0480021464.01	0.814055	1.872031e-51	1.243109e-48
MsG0280010177.01	MsG0480022052.01	-0.813299	2.746691e-51	1.786865e-48
MsG0280010177.01	MsG0480022164.01	0.830202	3.354590e-55	3.510547e-52
MsG0280010177.01	MsG0480022207.01	0.810151	1.329481e-50	7.953557e-48
MsG0280010177.01	MsG0480022223.01	0.823705	1.199680e-53	1.040961e-50
MsG0280010177.01	MsG0480022696.01	0.811839	5.728213e-51	3.583493e-48
MsG0280010177.01	MsG0580024051.01	0.807190	5.705810e-50	3.157603e-47
MsG0280010177.01	MsG0580024339.01	0.818100	2.338316e-52	1.733972e-49
MsG0280010177.01	MsG0580024727.01	0.803498	3.388307e-49	1.705353e-46
MsG0280010177.01	MsG0580025756.01	0.816829	4.519251e-52	3.236676e-49
MsG0280010177.01	MsG0580026301.01	0.806350	8.586599e-50	4.649967e-47
MsG0280010177.01	MsG0580026485.01	0.817607	3.021315e-52	2.210425e-49
MsG0280010177.01	MsG0580026718.01	0.810655	1.034899e-50	6.274496e-48
MsG0280010177.01	MsG0580027615.01	0.803764	2.983150e-49	1.511734e-46
MsG0280010177.01	MsG0580027925.01	0.800036	1.740121e-48	8.020040e-46
MsG0280010177.01	MsG0580028613.01	-0.803369	3.602034e-49	1.806841e-46
MsG0280010177.01	MsG0680031507.01	0.805676	1.189837e-49	6.333647e-47
MsG0280010177.01	MsG0680031628.01	0.833198	6.120230e-56	6.997973e-53
MsG0280010177.01	MsG0680035346.01	0.800596	1.338401e-48	6.256901e-46
MsG0280010177.01	MsG0780037240.01	0.823522	1.323590e-53	1.142390e-50
MsG0280010177.01	MsG0780040162.01	0.817906	2.585682e-52	1.907282e-49
MsG0280010177.01	MsG0780040545.01	0.821651	3.605905e-53	2.951161e-50
MsG0280010177.01	MsG0780041295.01	0.804234	2.382783e-49	1.222059e-46
MsG0280010177.01	MsG0780041392.01	0.814939	1.192976e-51	8.113519e-49
MsG0280010177.01	MsG0780041559.01	0.817847	2.666668e-52	1.963923e-49
MsG0280010177.01	MsG0880042779.01	0.832037	1.187930e-55	1.312199e-52
MsG0280010177.01	MsG0880043465.01	0.806068	9.844422e-50	5.293127e-47
MsG0280010177.01	MsG0880044975.01	0.819810	9.551343e-53	7.427114e-50
MsG0280010177.01	MsG0880045145.01	0.828307	9.667730e-55	9.572509e-52
MsG0280010177.01	MsG0880045965.01	0.822953	1.797133e-53	1.526378e-50
MsG0280010177.01	MsG0880047472.01	0.803376	3.590113e-49	1.801206e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010177.01.T01	MTR_2g078930	67.327	202	38	4	65	240	220	419	1.02e-74	235
MsG0280010177.01.T01	MTR_2g078930	74.576	59	15	0	1	59	31	89	7.02e-19	85.9
MsG0280010177.01.T01	MTR_2g078990	59.375	192	53	3	65	233	37	226	9.42e-64	202
MsG0280010177.01.T01	MTR_2g079020	58.854	192	54	3	65	233	214	403	1.19e-62	204
MsG0280010177.01.T01	MTR_2g079020	72.881	59	16	0	1	59	25	83	5.37e-18	83.6
MsG0280010177.01.T01	MTR_2g078960	61.979	192	48	3	65	233	210	399	1.38e-61	201
MsG0280010177.01.T01	MTR_2g078960	73.684	57	15	0	3	59	23	79	1.58e-17	82.0
MsG0280010177.01.T01	MTR_2g078970	57.292	192	57	3	65	233	208	397	3.09e-61	201
MsG0280010177.01.T01	MTR_2g078970	67.797	59	19	0	1	59	19	77	3.23e-16	78.2
MsG0280010177.01.T01	MTR_2g079030	86.000	100	14	0	136	235	349	448	3.62e-54	183
MsG0280010177.01.T01	MTR_1g108840	31.016	187	106	5	69	233	222	407	9.66e-18	82.8
MsG0280010177.01.T01	MTR_8g106670	26.000	200	101	3	70	235	194	380	2.20e-17	81.6
MsG0280010177.01.T01	MTR_1g108810	38.938	113	66	1	136	245	303	415	9.01e-17	79.7
MsG0280010177.01.T01	MTR_6g081240	29.949	197	111	6	61	233	183	376	9.51e-15	73.6
MsG0280010177.01.T01	MTR_6g081410	29.843	191	103	5	70	233	207	393	1.48e-14	73.2
MsG0280010177.01.T01	MTR_5g090290	29.744	195	106	4	65	233	223	412	1.54e-14	73.2
MsG0280010177.01.T01	MTR_6g081400	28.169	213	128	4	61	249	189	400	2.88e-14	72.4
MsG0280010177.01.T01	MTR_8g069470	37.363	91	57	0	143	233	325	415	5.39e-14	71.6
MsG0280010177.01.T01	MTR_3g099700	38.462	91	56	0	143	233	321	411	2.87e-13	69.3
MsG0280010177.01.T01	MTR_6g081810	29.878	164	110	1	70	233	209	367	3.13e-13	69.3
MsG0280010177.01.T01	MTR_6g081780	30.366	191	102	6	70	233	207	393	3.18e-13	69.3
MsG0280010177.01.T01	MTR_2g088400	25.134	187	117	3	69	233	233	418	6.80e-13	68.2
MsG0280010177.01.T01	MTR_1g100180	39.560	91	55	0	143	233	317	407	1.20e-12	67.8
MsG0280010177.01.T01	MTR_7g082810	39.130	92	56	0	142	233	295	386	1.33e-12	67.4
MsG0280010177.01.T01	MTR_2g088400	25.134	187	117	3	69	233	233	418	1.33e-12	67.4
MsG0280010177.01.T01	MTR_5g087930	29.630	189	95	5	64	233	202	371	1.58e-12	67.0
MsG0280010177.01.T01	MTR_6g081300	28.934	197	113	6	61	233	183	376	4.05e-12	65.9
MsG0280010177.01.T01	MTR_6g004220	36.735	98	62	0	136	233	300	397	4.17e-12	65.9
MsG0280010177.01.T01	MTR_6g004220	36.735	98	62	0	136	233	293	390	4.93e-12	65.9
MsG0280010177.01.T01	MTR_6g004220	36.735	98	62	0	136	233	300	397	5.58e-12	65.5
MsG0280010177.01.T01	MTR_6g081390	28.421	190	105	5	70	233	195	379	6.00e-12	65.5
MsG0280010177.01.T01	MTR_7g087370	41.837	98	57	0	136	233	268	365	9.66e-12	64.7
MsG0280010177.01.T01	MTR_4g048000	36.957	92	58	0	142	233	339	430	1.13e-11	64.7
MsG0280010177.01.T01	MTR_8g063590	27.778	180	108	4	76	233	241	420	2.13e-11	63.9
MsG0280010177.01.T01	MTR_5g032720	40.659	91	54	0	143	233	311	401	2.83e-11	63.5
MsG0280010177.01.T01	MTR_6g027200	37.363	91	57	0	143	233	311	401	4.26e-11	63.2
MsG0280010177.01.T01	MTR_7g087410	42.857	91	52	0	143	233	217	307	7.21e-11	62.0
MsG0280010177.01.T01	MTR_7g087410	42.857	91	52	0	143	233	306	396	8.87e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010177.01.T01	AT3G23560	46.073	191	74	3	68	233	218	404	5.08e-40	143
MsG0280010177.01.T01	AT3G23560	46.073	191	74	3	68	233	218	404	1.19e-39	143
MsG0280010177.01.T01	AT3G23550	42.056	214	98	5	61	249	202	414	3.89e-36	134
MsG0280010177.01.T01	AT2G34360	31.183	186	103	3	68	233	206	386	1.11e-17	82.4
MsG0280010177.01.T01	AT5G52450	31.383	188	106	3	68	233	205	391	8.00e-16	77.0
MsG0280010177.01.T01	AT1G11670	29.947	187	110	3	68	233	229	415	9.00e-16	77.0
MsG0280010177.01.T01	AT1G61890	41.489	94	55	0	140	233	319	412	2.64e-15	75.5
MsG0280010177.01.T01	AT1G61890	41.489	94	55	0	140	233	319	412	3.05e-15	75.5
MsG0280010177.01.T01	AT1G61890	41.489	94	55	0	140	233	319	412	3.42e-15	75.1
MsG0280010177.01.T01	AT1G23300	41.758	91	53	0	143	233	318	408	1.47e-14	73.2
MsG0280010177.01.T01	AT1G73700	31.053	190	100	4	70	233	205	389	3.75e-14	72.0
MsG0280010177.01.T01	AT1G33110	42.857	98	56	0	136	233	303	400	4.76e-14	71.6
MsG0280010177.01.T01	AT1G33110	42.857	98	56	0	136	233	303	400	5.69e-14	71.6
MsG0280010177.01.T01	AT2G34360	30.723	166	88	4	68	233	206	344	6.79e-14	71.2
MsG0280010177.01.T01	AT1G15150	30.481	187	107	3	69	233	211	396	8.59e-14	70.9
MsG0280010177.01.T01	AT1G15150	30.481	187	107	3	69	233	95	280	9.78e-14	70.5
MsG0280010177.01.T01	AT1G33100	43.956	91	51	0	143	233	307	397	1.34e-13	70.5
MsG0280010177.01.T01	AT4G00350	38.462	91	56	0	143	233	361	451	1.35e-13	70.5
MsG0280010177.01.T01	AT3G26590	40.659	91	54	0	143	233	217	307	1.60e-13	70.1
MsG0280010177.01.T01	AT3G26590	40.659	91	54	0	143	233	319	409	1.62e-13	70.1
MsG0280010177.01.T01	AT1G12950	38.710	93	57	0	141	233	339	431	3.94e-13	69.3
MsG0280010177.01.T01	AT1G15170	30.457	197	111	4	58	233	208	399	3.99e-13	68.9
MsG0280010177.01.T01	AT1G15170	30.457	197	111	4	58	233	89	280	4.47e-13	68.6
MsG0280010177.01.T01	AT5G38030	39.560	91	55	0	143	233	319	409	9.15e-13	68.2
MsG0280010177.01.T01	AT2G04040	27.500	200	121	4	70	247	208	405	1.55e-12	67.4
MsG0280010177.01.T01	AT1G15160	38.053	113	61	1	130	233	284	396	1.84e-12	67.0
MsG0280010177.01.T01	AT1G33090	43.878	98	55	0	136	233	303	400	1.99e-12	67.0
MsG0280010177.01.T01	AT4G25640	34.409	93	61	0	136	228	300	392	3.40e-12	66.2
MsG0280010177.01.T01	AT4G25640	34.409	93	61	0	136	228	300	392	3.56e-12	66.2
MsG0280010177.01.T01	AT4G25640	34.409	93	61	0	136	228	300	392	3.79e-12	66.2
MsG0280010177.01.T01	AT2G04050	31.443	194	94	8	70	233	208	392	4.82e-12	65.9
MsG0280010177.01.T01	AT1G15180	36.283	113	63	1	130	233	288	400	6.91e-12	65.5
MsG0280010177.01.T01	AT1G47530	37.168	113	71	0	121	233	195	307	7.04e-12	65.1
MsG0280010177.01.T01	AT2G04100	26.601	203	125	4	70	250	211	411	7.52e-12	65.1
MsG0280010177.01.T01	AT1G33080	39.796	98	59	0	136	233	303	400	7.61e-12	65.1
MsG0280010177.01.T01	AT2G04100	26.601	203	125	4	70	250	211	411	7.62e-12	65.1
MsG0280010177.01.T01	AT1G33080	39.796	98	59	0	136	233	303	400	8.09e-12	65.1
MsG0280010177.01.T01	AT1G47530	37.168	113	71	0	121	233	287	399	8.98e-12	65.1
MsG0280010177.01.T01	AT1G15180	36.283	113	63	1	130	233	168	280	1.10e-11	64.7
MsG0280010177.01.T01	AT3G21690	26.178	191	120	3	64	233	227	417	5.11e-11	62.8
MsG0280010177.01.T01	AT5G10420	37.363	91	57	0	143	233	312	402	5.17e-11	62.8
MsG0280010177.01.T01	AT2G04080	34.711	121	54	3	130	233	280	392	9.05e-11	62.0

Find 70 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTATGATGATGTAGGGTTTA+TGG	0.149157	2:+65770505	MsG0280010177.01.T01:intron
GGTGCTACTCTTGCTAATTC+TGG	0.205952	2:+65766004	MsG0280010177.01.T01:CDS
ATTGCATGTTTAGCTCTTTC+AGG	0.235007	2:-65769750	None:intergenic
TGTGGCCTAGTGTGTCAAAT+TGG	0.269313	2:+65770540	MsG0280010177.01.T01:CDS
CACAAGGATTTCCAATGAAT+TGG	0.272145	2:+65769716	MsG0280010177.01.T01:CDS
AACAAAGTTGCTAAATTATT+AGG	0.272351	2:+65768853	MsG0280010177.01.T01:CDS
ATTGCTTACATGATCACTTA+TGG	0.287008	2:+65769535	MsG0280010177.01.T01:CDS
GATATTCAGTTTAGTCCATT+TGG	0.317780	2:-65770589	None:intergenic
TTGGTTTCTGTCATGCTTGT+TGG	0.351093	2:+65765956	MsG0280010177.01.T01:CDS
TGATAGTCCTATTATCAAAA+AGG	0.356312	2:+65769863	MsG0280010177.01.T01:CDS
TTGCAACTTCTATTTCACTA+TGG	0.356695	2:+65768637	MsG0280010177.01.T01:CDS
ACAAGGATTTCCAATGAATT+GGG	0.362220	2:+65769717	MsG0280010177.01.T01:CDS
TTATGGATTGGTCTAATTTG+TGG	0.372381	2:+65770522	MsG0280010177.01.T01:CDS
TTGGACAGAATCTAGTAGTA+TGG	0.375092	2:-65769919	None:intergenic
CTTGGTGAGCTTCAGTTAGC+TGG	0.379613	2:+65765983	MsG0280010177.01.T01:CDS
CGGGGCATTGGTAACTCAAC+TGG	0.382594	2:-65768904	None:intergenic
TCTGTCCAAGGTGTCTTATC+AGG	0.399701	2:+65769933	MsG0280010177.01.T01:CDS
GTGGCCTAGTGTGTCAAATT+GGG	0.408419	2:+65770541	MsG0280010177.01.T01:CDS
GAAAACATGAGTTGTTGTTT+TGG	0.413443	2:-65765891	None:intergenic
CTAAAGCTCTCTGTCCTCCT+TGG	0.415938	2:+65769783	MsG0280010177.01.T01:CDS
ATTAGCTCTGATACCATGTT+AGG	0.420515	2:-65770822	None:intergenic
ACATGGTATCAGAGCTAATC+TGG	0.421300	2:+65770826	MsG0280010177.01.T01:three_prime_UTR
ATGATGAAGTTATCCACTAA+TGG	0.435425	2:-65770726	None:intergenic
GTCATGCTTGTTGGTCACCT+TGG	0.441677	2:+65765965	MsG0280010177.01.T01:CDS
ATGGACAGGTTTGAGTTCAT+AGG	0.444229	2:+65768606	MsG0280010177.01.T01:intron
ATACTTGTAATAACCATTAG+TGG	0.446701	2:+65770713	MsG0280010177.01.T01:three_prime_UTR
CAAATGGTGGAACAAAATCT+TGG	0.453309	2:+65765856	MsG0280010177.01.T01:CDS
TATTTATTACTTGATTACTT+TGG	0.454413	2:+65765937	MsG0280010177.01.T01:CDS
TAATTCTGGTTTAGTGTCAC+TGG	0.460003	2:+65766018	MsG0280010177.01.T01:CDS
AACTAAAGCTACCGGGGCAT+TGG	0.463556	2:-65768916	None:intergenic
GACCAGAAATGGAGGACAAA+TGG	0.466040	2:+65765840	None:intergenic
TAATCTGGTATCCACCTTGA+AGG	0.466612	2:+65770841	MsG0280010177.01.T01:three_prime_UTR
GTTGTTATACAGCAATAAGT+TGG	0.467334	2:-65770630	None:intergenic
ATGGACTAAACTGAATATCT+CGG	0.497360	2:+65770593	MsG0280010177.01.T01:exon
GCAGCGGAAAATATCCTTCA+AGG	0.505081	2:-65770855	None:intergenic
GGATATCATGTTTCCTAACA+TGG	0.513001	2:+65770809	MsG0280010177.01.T01:three_prime_UTR
AAATGTTGCTCAAAGTAAAC+CGG	0.514050	2:-65768876	None:intergenic
TGGATATCTATGATGTTGGT+AGG	0.516691	2:+65768657	MsG0280010177.01.T01:CDS
AGTGAAATAGAAGTTGCAAC+TGG	0.517295	2:-65768633	None:intergenic
ACTATGGATATCTATGATGT+TGG	0.525384	2:+65768653	MsG0280010177.01.T01:CDS
CATGTTTAGCTCTTTCAGGA+TGG	0.526642	2:-65769746	None:intergenic
TGGACTAAACTGAATATCTC+GGG	0.533874	2:+65770594	MsG0280010177.01.T01:exon
GCAAAGAAGTTGAGAATACA+TGG	0.543684	2:+65768696	MsG0280010177.01.T01:CDS
TCAAAACAAAACAGAATCCA+AGG	0.547423	2:-65769800	None:intergenic
ATGATGTAGGGTTTATGGAT+TGG	0.565269	2:+65770510	MsG0280010177.01.T01:intron
AAGTTGCTAAATTATTAGGC+CGG	0.566905	2:+65768857	MsG0280010177.01.T01:CDS
TGGCCTAGTGTGTCAAATTG+GGG	0.576838	2:+65770542	MsG0280010177.01.T01:CDS
CAAGGATTTCCAATGAATTG+GGG	0.583001	2:+65769718	MsG0280010177.01.T01:CDS
ATATACTAATAGCTGCCACT+TGG	0.586541	2:-65770690	None:intergenic
GTTAGGAAACATGATATCCA+AGG	0.593694	2:-65770805	None:intergenic
ATACTACTAGATTCTGTCCA+AGG	0.600992	2:+65769921	MsG0280010177.01.T01:CDS
GACAGGTTTGAGTTCATAGG+TGG	0.606806	2:+65768609	MsG0280010177.01.T01:intron
CAGAAATGGAGGACAAATGG+TGG	0.611520	2:+65765843	None:intergenic
GTGGAACAAAATCTTGGACA+TGG	0.619014	2:+65765862	MsG0280010177.01.T01:CDS
TCATACCTGATAAGACACCT+TGG	0.620326	2:-65769938	None:intergenic
GAAGTTGAGAATACATGGAG+AGG	0.625235	2:+65768701	MsG0280010177.01.T01:CDS
GGAAACATGATATCCAAGGA+AGG	0.626961	2:-65770801	None:intergenic
ATGGTCTTAGTGCAGCTGCA+AGG	0.630564	2:+65769554	MsG0280010177.01.T01:CDS
CAGTTGAGTTACCAATGCCC+CGG	0.639829	2:+65768905	MsG0280010177.01.T01:CDS
AGTATGGAAATAGCAAGCAA+AGG	0.641984	2:-65769903	None:intergenic
TAGGACTGAATATGTATGTG+TGG	0.645601	2:+65770664	MsG0280010177.01.T01:three_prime_UTR
AAGGATTTCCAATGAATTGG+GGG	0.656984	2:+65769719	MsG0280010177.01.T01:CDS
GTGCCCCAATTTGACACACT+AGG	0.664260	2:-65770545	None:intergenic
ATAACAACTGAACTATACGT+AGG	0.698814	2:+65770645	MsG0280010177.01.T01:three_prime_UTR
AGGACTGAATATGTATGTGT+GGG	0.705374	2:+65770665	MsG0280010177.01.T01:three_prime_UTR
GCGGAAAATATCCTTCAAGG+TGG	0.709379	2:-65770852	None:intergenic
CAGCTAACTGAAGCTCACCA+AGG	0.723214	2:-65765982	None:intergenic
GAACAAAATCTTGGACATGG+AGG	0.742594	2:+65765865	MsG0280010177.01.T01:CDS
ATGTATGTGTGGGAACCAAG+TGG	0.753205	2:+65770675	MsG0280010177.01.T01:three_prime_UTR
AAACAAAACAGAATCCAAGG+AGG	0.770094	2:-65769797	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATTATACTCTATAAT+GGG	-	Chr2:65766949-65766968	None:intergenic	10.0%
!!	AAAATTATAATAAAGAATTG+AGG	-	Chr2:65766380-65766399	None:intergenic	10.0%
!!	TATTTATTTCATTATAAAAG+TGG	-	Chr2:65767056-65767075	None:intergenic	10.0%
!!!	ATAAACTTTTTCTTATAATA+AGG	-	Chr2:65766639-65766658	None:intergenic	10.0%
!!!	ATTATAGAGTATAATATTTT+GGG	+	Chr2:65766949-65766968	MsG0280010177.01.T01:intron	10.0%
!!	AAATAGTAAGATTCATAATA+AGG	-	Chr2:65769310-65769329	None:intergenic	15.0%
!!	AAATATTATACTCTATAATG+GGG	-	Chr2:65766948-65766967	None:intergenic	15.0%
!!	AATAAGTTTGTAAAAATCAT+TGG	-	Chr2:65765921-65765940	None:intergenic	15.0%
!!	ATAATCAATTGAAGTAAATT+TGG	+	Chr2:65767341-65767360	MsG0280010177.01.T01:intron	15.0%
!!	CAAAATATTATACTCTATAA+TGG	-	Chr2:65766950-65766969	None:intergenic	15.0%
!!	GCATAAACATTTATATTTAT+TGG	-	Chr2:65766281-65766300	None:intergenic	15.0%
!!	TAATAAAACAAAAATTAGTG+AGG	-	Chr2:65767612-65767631	None:intergenic	15.0%
!!	TAATCAATTGAAGTAAATTT+GGG	+	Chr2:65767342-65767361	MsG0280010177.01.T01:intron	15.0%
!!	TATTTAACACCATATTAAAT+AGG	+	Chr2:65766920-65766939	MsG0280010177.01.T01:intron	15.0%
!!	TATTTATTACTTGATTACTT+TGG	+	Chr2:65765937-65765956	MsG0280010177.01.T01:CDS	15.0%
!!	TCATAAAATTAGTTCAATTA+AGG	+	Chr2:65770402-65770421	MsG0280010177.01.T01:intron	15.0%
!!	TCTTATATTTGATTCTTAAA+TGG	-	Chr2:65767207-65767226	None:intergenic	15.0%
!!!	AAATTTGTTTATATGAGAAT+TGG	+	Chr2:65767909-65767928	MsG0280010177.01.T01:intron	15.0%
!!!	ATTGATCATTTAAGTTATTT+TGG	+	Chr2:65769012-65769031	MsG0280010177.01.T01:intron	15.0%
!!!	CATTATAGAGTATAATATTT+TGG	+	Chr2:65766948-65766967	MsG0280010177.01.T01:intron	15.0%
!!!	TATTTAATATGGTGTTAAAT+AGG	-	Chr2:65766921-65766940	None:intergenic	15.0%
!!!	TATTTCACATACTTATTTTA+CGG	-	Chr2:65767178-65767197	None:intergenic	15.0%
!!!	TTTTAGTAAAAATCTCTTTT+CGG	-	Chr2:65770258-65770277	None:intergenic	15.0%
!!	AAAGAACATAGTTTAATCTT+GGG	-	Chr2:65768215-65768234	None:intergenic	20.0%
!!	AAATGAACAAATAAAGTGAA+AGG	-	Chr2:65768816-65768835	None:intergenic	20.0%
!!	AACAAAGTTGCTAAATTATT+AGG	+	Chr2:65768853-65768872	MsG0280010177.01.T01:CDS	20.0%
!!	ATTTCAAAGGAGATTATTTA+TGG	-	Chr2:65767258-65767277	None:intergenic	20.0%
!!	CATTGAATAACTAATGTATT+TGG	-	Chr2:65766708-65766727	None:intergenic	20.0%
!!	TATATATTAGACTTTGATTG+TGG	+	Chr2:65768946-65768965	MsG0280010177.01.T01:intron	20.0%
!!	TCATCATTATATCAATTGTT+TGG	+	Chr2:65768367-65768386	MsG0280010177.01.T01:intron	20.0%
!!	TCATTGACAAAAAAATCTTA+GGG	+	Chr2:65770004-65770023	MsG0280010177.01.T01:intron	20.0%
!!	TTATATTAAAGAATGCTCTA+AGG	+	Chr2:65767443-65767462	MsG0280010177.01.T01:intron	20.0%
!!	TTCATTGACAAAAAAATCTT+AGG	+	Chr2:65770003-65770022	MsG0280010177.01.T01:intron	20.0%
!!!	AATTTCATTTGAATTTTGGT+TGG	+	Chr2:65767126-65767145	MsG0280010177.01.T01:intron	20.0%
!!!	ACTTGATTCTTATTTTTTGT+AGG	+	Chr2:65766817-65766836	MsG0280010177.01.T01:intron	20.0%
!!!	CAGTAATTTCATTTGAATTT+TGG	+	Chr2:65767122-65767141	MsG0280010177.01.T01:intron	20.0%
!!!	CTTGATTCTTATTTTTTGTA+GGG	+	Chr2:65766818-65766837	MsG0280010177.01.T01:intron	20.0%
!!!	TAAACAAAAATTCTCTTTTC+AGG	-	Chr2:65770350-65770369	None:intergenic	20.0%
!!!	TATTGTCCTTTAAGTTATTT+TGG	-	Chr2:65769225-65769244	None:intergenic	20.0%
!!!	TTAGTTGCAGTTTATTTTAT+GGG	-	Chr2:65768000-65768019	None:intergenic	20.0%
!!!	TTATTTTGTATTATGCAAGT+TGG	+	Chr2:65769330-65769349	MsG0280010177.01.T01:intron	20.0%
!!!	TTTTCGTGCATAATTAATAA+AGG	+	Chr2:65767419-65767438	MsG0280010177.01.T01:intron	20.0%
!	AAATATAAGATGCTATGTTG+TGG	+	Chr2:65767217-65767236	MsG0280010177.01.T01:intron	25.0%
!	AAGTTGTTGCAAACATTAAT+GGG	-	Chr2:65767292-65767311	None:intergenic	25.0%
!	ACAAAACAACAAAACAAATG+GGG	-	Chr2:65767878-65767897	None:intergenic	25.0%
!	AGTTTAACCTTGTAAATTCA+AGG	+	Chr2:65768045-65768064	MsG0280010177.01.T01:intron	25.0%
!	ATACTTGTAATAACCATTAG+TGG	+	Chr2:65770713-65770732	MsG0280010177.01.T01:three_prime_UTR	25.0%
!	CAAAGAACATAGTTTAATCT+TGG	-	Chr2:65768216-65768235	None:intergenic	25.0%
!	CACAAAACAACAAAACAAAT+GGG	-	Chr2:65767879-65767898	None:intergenic	25.0%
!	CATGATCAACATATGTAAAA+TGG	-	Chr2:65767691-65767710	None:intergenic	25.0%
!	CATTGACAAAAAAATCTTAG+GGG	+	Chr2:65770005-65770024	MsG0280010177.01.T01:intron	25.0%
!	GAAAACGTTTACAAACTTAA+AGG	-	Chr2:65769049-65769068	None:intergenic	25.0%
!	GTATGTTCATCATAAACTTT+AGG	+	Chr2:65767664-65767683	MsG0280010177.01.T01:intron	25.0%
!	GTCTGAATATGTAAAATTAG+AGG	+	Chr2:65768146-65768165	MsG0280010177.01.T01:intron	25.0%
!	TAAACCAATGAGATAAAAAG+TGG	-	Chr2:65770106-65770125	None:intergenic	25.0%
!	TAAGACCAATTTGCATTATA+AGG	+	Chr2:65770149-65770168	MsG0280010177.01.T01:intron	25.0%
!	TACTTTCTTAAATCACTACT+AGG	+	Chr2:65767501-65767520	MsG0280010177.01.T01:intron	25.0%
!	TATATGAAGTTTCTTATACC+AGG	+	Chr2:65768456-65768475	MsG0280010177.01.T01:intron	25.0%
!	TCACAAAACAACAAAACAAA+TGG	-	Chr2:65767880-65767899	None:intergenic	25.0%
!	TGATACCTTATAATGCAAAT+TGG	-	Chr2:65770157-65770176	None:intergenic	25.0%
!	TGATAGTCCTATTATCAAAA+AGG	+	Chr2:65769863-65769882	MsG0280010177.01.T01:CDS	25.0%
!!	CAATTTCATTGATTGCAATA+TGG	+	Chr2:65769412-65769431	MsG0280010177.01.T01:intron	25.0%
!!	CATAAGTTCATTTAAGTTTG+AGG	-	Chr2:65769197-65769216	None:intergenic	25.0%
!!	CATATAAACAAATTTTGCAG+AGG	-	Chr2:65767904-65767923	None:intergenic	25.0%
!!	TAATAGTGATAAGAGTATGT+AGG	+	Chr2:65770370-65770389	MsG0280010177.01.T01:intron	25.0%
!!!	AATTTTGTCTATGATGATGT+AGG	+	Chr2:65770497-65770516	MsG0280010177.01.T01:intron	25.0%
!!!	ATTTTGTCTATGATGATGTA+GGG	+	Chr2:65770498-65770517	MsG0280010177.01.T01:intron	25.0%
!!!	GTTAGTTGCAGTTTATTTTA+TGG	-	Chr2:65768001-65768020	None:intergenic	25.0%
!!!	TTGAACTTTTTTATAGCACA+AGG	+	Chr2:65769700-65769719	MsG0280010177.01.T01:intron	25.0%
!!!	TTGCTTTAAAAAGACTTAAG+AGG	+	Chr2:65766561-65766580	MsG0280010177.01.T01:intron	25.0%
!!!	TTTGTTTTGATGCTTTTGTT+TGG	+	Chr2:65769813-65769832	MsG0280010177.01.T01:CDS	25.0%
	AAAATCCCTCACAAATTCTT+TGG	+	Chr2:65767530-65767549	MsG0280010177.01.T01:intron	30.0%
	AAAATGTCTTGCATAATGCA+GGG	+	Chr2:65770029-65770048	MsG0280010177.01.T01:intron	30.0%
	AAATGTTGCTCAAAGTAAAC+CGG	-	Chr2:65768879-65768898	None:intergenic	30.0%
	AACAAAGTCTAAATTACACC+AGG	-	Chr2:65769273-65769292	None:intergenic	30.0%
	AAGTTGCTAAATTATTAGGC+CGG	+	Chr2:65768857-65768876	MsG0280010177.01.T01:CDS	30.0%
	AAGTTTATGATGAACATACG+TGG	-	Chr2:65767663-65767682	None:intergenic	30.0%
	AATAAAAATGACCCCTATGA+AGG	-	Chr2:65769608-65769627	None:intergenic	30.0%
	AATAAACTGCAACTAACAAG+AGG	+	Chr2:65768004-65768023	MsG0280010177.01.T01:intron	30.0%
	AATCCAACATGAATCAATGT+TGG	-	Chr2:65768569-65768588	None:intergenic	30.0%
	ACAAAGTCTAAATTACACCA+GGG	-	Chr2:65769272-65769291	None:intergenic	30.0%
	ACAAGGATTTCCAATGAATT+GGG	+	Chr2:65769717-65769736	MsG0280010177.01.T01:CDS	30.0%
	ACTATGGATATCTATGATGT+TGG	+	Chr2:65768653-65768672	MsG0280010177.01.T01:CDS	30.0%
	ACTTTAAATTTCCTCGAACA+TGG	+	Chr2:65768073-65768092	MsG0280010177.01.T01:intron	30.0%
	ATAACAACTGAACTATACGT+AGG	+	Chr2:65770645-65770664	MsG0280010177.01.T01:three_prime_UTR	30.0%
	ATCCAACATGAATCAATGTT+GGG	-	Chr2:65768568-65768587	None:intergenic	30.0%
	ATGATGAAGTTATCCACTAA+TGG	-	Chr2:65770729-65770748	None:intergenic	30.0%
	ATTGCTTACATGATCACTTA+TGG	+	Chr2:65769535-65769554	MsG0280010177.01.T01:CDS	30.0%
	ATTGTGGCTAAATTACATCT+GGG	+	Chr2:65768962-65768981	MsG0280010177.01.T01:intron	30.0%
	ATTGTTCCTATCAACATCTA+TGG	-	Chr2:65766100-65766119	None:intergenic	30.0%
	ATTTATTTCACGCAAATTGG+AGG	+	Chr2:65767964-65767983	MsG0280010177.01.T01:intron	30.0%
	CAAGTTGTTGCAAACATTAA+TGG	-	Chr2:65767293-65767312	None:intergenic	30.0%
	GATATTCAGTTTAGTCCATT+TGG	-	Chr2:65770592-65770611	None:intergenic	30.0%
	GTCATTTATTTCACGCAAAT+TGG	+	Chr2:65767961-65767980	MsG0280010177.01.T01:intron	30.0%
	GTGTGACCAAAATAACTTAA+AGG	+	Chr2:65769216-65769235	MsG0280010177.01.T01:intron	30.0%
	GTTCATACCTTGAATTTACA+AGG	-	Chr2:65768055-65768074	None:intergenic	30.0%
	GTTGTTATACAGCAATAAGT+TGG	-	Chr2:65770633-65770652	None:intergenic	30.0%
	TAAAATGTCTTGCATAATGC+AGG	+	Chr2:65770028-65770047	MsG0280010177.01.T01:intron	30.0%
	TAGTGAGGAAAATTTAGAGA+GGG	-	Chr2:65767597-65767616	None:intergenic	30.0%
	TCAAAACAAAACAGAATCCA+AGG	-	Chr2:65769803-65769822	None:intergenic	30.0%
	TCATGACGCTATACTTAATT+TGG	-	Chr2:65766764-65766783	None:intergenic	30.0%
	TCATTTCCATAGATGTTGAT+AGG	+	Chr2:65766091-65766110	MsG0280010177.01.T01:intron	30.0%
	TTAGTGAGGAAAATTTAGAG+AGG	-	Chr2:65767598-65767617	None:intergenic	30.0%
	TTGCAACTTCTATTTCACTA+TGG	+	Chr2:65768637-65768656	MsG0280010177.01.T01:CDS	30.0%
!	ATGGACTAAACTGAATATCT+CGG	+	Chr2:65770593-65770612	MsG0280010177.01.T01:exon	30.0%
!	ATGTTGATAGGAACAATTCT+TGG	+	Chr2:65766103-65766122	MsG0280010177.01.T01:intron	30.0%
!	ATTATGCAAGTTGGAGTATT+GGG	+	Chr2:65769339-65769358	MsG0280010177.01.T01:intron	30.0%
!	TAAGTTTGAGGTTGTAACAT+TGG	-	Chr2:65769185-65769204	None:intergenic	30.0%
!	TATTATGCAAGTTGGAGTAT+TGG	+	Chr2:65769338-65769357	MsG0280010177.01.T01:intron	30.0%
!	TCATTGATTGCAATATGGTA+TGG	+	Chr2:65769417-65769436	MsG0280010177.01.T01:intron	30.0%
!	TCTGGGATCATTTAAGTTTA+AGG	+	Chr2:65768979-65768998	MsG0280010177.01.T01:intron	30.0%
!	TGTTGATAGGAACAATTCTT+GGG	+	Chr2:65766104-65766123	MsG0280010177.01.T01:intron	30.0%
!	TTGCATATGGATTTGTTCAA+TGG	+	Chr2:65768587-65768606	MsG0280010177.01.T01:intron	30.0%
!	TTGGTGGCAAAATTTAGTTA+CGG	+	Chr2:65767549-65767568	MsG0280010177.01.T01:intron	30.0%
!	TTTGTTACCATTGATGCTTT+TGG	-	Chr2:65767320-65767339	None:intergenic	30.0%
!!	ATCATCGTTTTTGTAACGTT+TGG	-	Chr2:65769091-65769110	None:intergenic	30.0%
!!	GAAATTGTTATTTGTGAGTC+AGG	-	Chr2:65769399-65769418	None:intergenic	30.0%
!!	GCAAACTCCTTTTTGATAAT+AGG	-	Chr2:65769873-65769892	None:intergenic	30.0%
!!	TAATTTTTCAACCATGTTCG+AGG	-	Chr2:65768087-65768106	None:intergenic	30.0%
!!	TGGGAAAGTACTAGTAAATT+GGG	+	Chr2:65767361-65767380	MsG0280010177.01.T01:intron	30.0%
!!	TTATGGATTGGTCTAATTTG+TGG	+	Chr2:65770522-65770541	MsG0280010177.01.T01:CDS	30.0%
!!	TTGGGAAAGTACTAGTAAAT+TGG	+	Chr2:65767360-65767379	MsG0280010177.01.T01:intron	30.0%
!!!	AGTATTGGGCTTTTGAAATT+TGG	+	Chr2:65769353-65769372	MsG0280010177.01.T01:intron	30.0%
!!!	GAAAACATGAGTTGTTGTTT+TGG	-	Chr2:65765894-65765913	None:intergenic	30.0%
!!!	GAATTTTGGTTGGAGAAAAT+TGG	+	Chr2:65767136-65767155	MsG0280010177.01.T01:intron	30.0%
!!!	TGAAATTTGGTTTTCTTAGC+TGG	+	Chr2:65769366-65769385	MsG0280010177.01.T01:intron	30.0%
!!!	TTCATAGGGGTCATTTTTAT+TGG	+	Chr2:65769607-65769626	MsG0280010177.01.T01:intron	30.0%
	AAACAAAACAGAATCCAAGG+AGG	-	Chr2:65769800-65769819	None:intergenic	35.0%
	AAATGTCTTGCATAATGCAG+GGG	+	Chr2:65770030-65770049	MsG0280010177.01.T01:intron	35.0%
	AACAGAATCCATTCCTTCAT+AGG	+	Chr2:65769592-65769611	MsG0280010177.01.T01:intron	35.0%
	AAGGATTTCCAATGAATTGG+GGG	+	Chr2:65769719-65769738	MsG0280010177.01.T01:CDS	35.0%
	AATCCAAGGAGACATGAAAT+TGG	-	Chr2:65770129-65770148	None:intergenic	35.0%
	AATGGGGAGCCTATTTAATA+TGG	-	Chr2:65766932-65766951	None:intergenic	35.0%
	ACAGAATCCATTCCTTCATA+GGG	+	Chr2:65769593-65769612	MsG0280010177.01.T01:intron	35.0%
	ACGCTATATGCAAATAATCC+TGG	-	Chr2:65768477-65768496	None:intergenic	35.0%
	ACGTTACAAAAACGATGATG+TGG	+	Chr2:65769092-65769111	MsG0280010177.01.T01:intron	35.0%
	AGGACTGAATATGTATGTGT+GGG	+	Chr2:65770665-65770684	MsG0280010177.01.T01:three_prime_UTR	35.0%
	AGTATGGAAATAGCAAGCAA+AGG	-	Chr2:65769906-65769925	None:intergenic	35.0%
	AGTGAAATAGAAGTTGCAAC+TGG	-	Chr2:65768636-65768655	None:intergenic	35.0%
	AGTGTTCAAACTAAAGCTAC+CGG	-	Chr2:65768927-65768946	None:intergenic	35.0%
	ATACTACTAGATTCTGTCCA+AGG	+	Chr2:65769921-65769940	MsG0280010177.01.T01:CDS	35.0%
	ATATACTAATAGCTGCCACT+TGG	-	Chr2:65770693-65770712	None:intergenic	35.0%
	ATGATGAACATACGTGGAAA+TGG	-	Chr2:65767657-65767676	None:intergenic	35.0%
	ATGATGTAGGGTTTATGGAT+TGG	+	Chr2:65770510-65770529	MsG0280010177.01.T01:intron	35.0%
	ATGCTGGGAATTTATCTACA+AGG	+	Chr2:65768320-65768339	MsG0280010177.01.T01:intron	35.0%
	CAAGGATTTCCAATGAATTG+GGG	+	Chr2:65769718-65769737	MsG0280010177.01.T01:CDS	35.0%
	CAATGAGATAAAAAGTGGCA+AGG	-	Chr2:65770101-65770120	None:intergenic	35.0%
	CACAAGGATTTCCAATGAAT+TGG	+	Chr2:65769716-65769735	MsG0280010177.01.T01:CDS	35.0%
	CACGGACAATCAAAAACATA+TGG	+	Chr2:65766142-65766161	MsG0280010177.01.T01:intron	35.0%
	GATTCATGTTGGATTGCATA+TGG	+	Chr2:65768574-65768593	MsG0280010177.01.T01:intron	35.0%
	GATTGTGGCTAAATTACATC+TGG	+	Chr2:65768961-65768980	MsG0280010177.01.T01:intron	35.0%
	GCAAAGAAGTTGAGAATACA+TGG	+	Chr2:65768696-65768715	MsG0280010177.01.T01:CDS	35.0%
	GCTGAAGTTAAAAAGAGATG+AGG	-	Chr2:65769967-65769986	None:intergenic	35.0%
	GGATATCATGTTTCCTAACA+TGG	+	Chr2:65770809-65770828	MsG0280010177.01.T01:three_prime_UTR	35.0%
	GTTAGGAAACATGATATCCA+AGG	-	Chr2:65770808-65770827	None:intergenic	35.0%
	TAGGACTGAATATGTATGTG+TGG	+	Chr2:65770664-65770683	MsG0280010177.01.T01:three_prime_UTR	35.0%
	TGGACTAAACTGAATATCTC+GGG	+	Chr2:65770594-65770613	MsG0280010177.01.T01:exon	35.0%
	TGGATATCTATGATGTTGGT+AGG	+	Chr2:65768657-65768676	MsG0280010177.01.T01:CDS	35.0%
	TTACCAATTTCATGTCTCCT+TGG	+	Chr2:65770123-65770142	MsG0280010177.01.T01:intron	35.0%
	TTGGACAGAATCTAGTAGTA+TGG	-	Chr2:65769922-65769941	None:intergenic	35.0%
!	AAGCCTTTTATTCTACCAAG+TGG	-	Chr2:65766796-65766815	None:intergenic	35.0%
!	CAAATGGTGGAACAAAATCT+TGG	+	Chr2:65765856-65765875	MsG0280010177.01.T01:CDS	35.0%
!	CTATGATGATGTAGGGTTTA+TGG	+	Chr2:65770505-65770524	MsG0280010177.01.T01:intron	35.0%
!	TACATGGAGAGGATTTTCAA+TGG	+	Chr2:65768712-65768731	MsG0280010177.01.T01:CDS	35.0%
!	TATGGATTTGTTCAATGGAC+AGG	+	Chr2:65768592-65768611	MsG0280010177.01.T01:intron	35.0%
!!	ATCCCAACATTGATTCATGT+TGG	+	Chr2:65768563-65768582	MsG0280010177.01.T01:intron	35.0%
!!	ATTAGCTCTGATACCATGTT+AGG	-	Chr2:65770825-65770844	None:intergenic	35.0%
!!	ATTGCATGTTTAGCTCTTTC+AGG	-	Chr2:65769753-65769772	None:intergenic	35.0%
!!	GCAAATTGGTCTTAAATCCA+AGG	-	Chr2:65770143-65770162	None:intergenic	35.0%
!!	TAATTCTGGTTTAGTGTCAC+TGG	+	Chr2:65766018-65766037	MsG0280010177.01.T01:CDS	35.0%
!!	TTGTCTTAGGTTGGTTTAAG+TGG	+	Chr2:65768248-65768267	MsG0280010177.01.T01:intron	35.0%
!!!	CTTGCCACTTTTTATCTCAT+TGG	+	Chr2:65770099-65770118	MsG0280010177.01.T01:intron	35.0%
	AAATGACCCCTATGAAGGAA+TGG	-	Chr2:65769603-65769622	None:intergenic	40.0%
	AAGTATAGCGTCATGATGTC+CGG	+	Chr2:65766768-65766787	MsG0280010177.01.T01:intron	40.0%
	ACATGGTATCAGAGCTAATC+TGG	+	Chr2:65770826-65770845	MsG0280010177.01.T01:three_prime_UTR	40.0%
	ACCTAAGACAAAGAAGAGCA+AGG	-	Chr2:65768239-65768258	None:intergenic	40.0%
	ATAGCTGAAAGGATAACCAG+TGG	-	Chr2:65768545-65768564	None:intergenic	40.0%
	ATCCCTCACAAATTCTTTGG+TGG	+	Chr2:65767533-65767552	MsG0280010177.01.T01:intron	40.0%
	CAGAATCCATTCCTTCATAG+GGG	+	Chr2:65769594-65769613	MsG0280010177.01.T01:intron	40.0%
	GAAACACTCGTCATTGTAGA+TGG	-	Chr2:65766897-65766916	None:intergenic	40.0%
	GAACAAAATCTTGGACATGG+AGG	+	Chr2:65765865-65765884	MsG0280010177.01.T01:CDS	40.0%
	GAAGTTGAGAATACATGGAG+AGG	+	Chr2:65768701-65768720	MsG0280010177.01.T01:CDS	40.0%
	GCAAAGGAATATCACATGCT+GGG	+	Chr2:65768305-65768324	MsG0280010177.01.T01:intron	40.0%
	GCTCCACTTGGTAGAATAAA+AGG	+	Chr2:65766790-65766809	MsG0280010177.01.T01:intron	40.0%
	GGAAACATGATATCCAAGGA+AGG	-	Chr2:65770804-65770823	None:intergenic	40.0%
	GTGGAACAAAATCTTGGACA+TGG	+	Chr2:65765862-65765881	MsG0280010177.01.T01:CDS	40.0%
	GTGTTCAAACTAAAGCTACC+GGG	-	Chr2:65768926-65768945	None:intergenic	40.0%
	TAATCTGGTATCCACCTTGA+AGG	+	Chr2:65770841-65770860	MsG0280010177.01.T01:three_prime_UTR	40.0%
	TCAATGTTGGGATAGCTGAA+AGG	-	Chr2:65768556-65768575	None:intergenic	40.0%
	TCACACTACTTAAAAGACCC+TGG	+	Chr2:65769252-65769271	MsG0280010177.01.T01:intron	40.0%
	TCATACCTGATAAGACACCT+TGG	-	Chr2:65769941-65769960	None:intergenic	40.0%
	TGCAAAGGAATATCACATGC+TGG	+	Chr2:65768304-65768323	MsG0280010177.01.T01:intron	40.0%
	TGCCACCAAAGAATTTGTGA+GGG	-	Chr2:65767538-65767557	None:intergenic	40.0%
	TGCTCTTCTTTGTCTTAGGT+TGG	+	Chr2:65768239-65768258	MsG0280010177.01.T01:intron	40.0%
	TGTTCAAACTAAAGCTACCG+GGG	-	Chr2:65768925-65768944	None:intergenic	40.0%
	TTCTAGCAATGTCTGGTGAT+TGG	-	Chr2:65768425-65768444	None:intergenic	40.0%
	TTGCCACCAAAGAATTTGTG+AGG	-	Chr2:65767539-65767558	None:intergenic	40.0%
	TTGGTTTCTGTCATGCTTGT+TGG	+	Chr2:65765956-65765975	MsG0280010177.01.T01:CDS	40.0%
	TTTATTCTACCAAGTGGAGC+CGG	-	Chr2:65766790-65766809	None:intergenic	40.0%
!	ATGGACAGGTTTGAGTTCAT+AGG	+	Chr2:65768606-65768625	MsG0280010177.01.T01:intron	40.0%
!	GGGTGTTCATTGATTAGACA+CGG	+	Chr2:65766124-65766143	MsG0280010177.01.T01:intron	40.0%
!	GTTACCATTGATGCTTTTGG+AGG	-	Chr2:65767317-65767336	None:intergenic	40.0%
!	GTTTTCTCTGATTCCTTCCT+TGG	+	Chr2:65770788-65770807	MsG0280010177.01.T01:three_prime_UTR	40.0%
!	TGCAAACATTAATGGGCCAT+TGG	-	Chr2:65767285-65767304	None:intergenic	40.0%
!	TGTAACGTTTGGTTCGCATA+GGG	-	Chr2:65769080-65769099	None:intergenic	40.0%
!	TTGTAACGTTTGGTTCGCAT+AGG	-	Chr2:65769081-65769100	None:intergenic	40.0%
!	TTTTGACAAGGCATGCCAAA+TGG	+	Chr2:65770574-65770593	MsG0280010177.01.T01:CDS	40.0%
!!	CATGTTTAGCTCTTTCAGGA+TGG	-	Chr2:65769749-65769768	None:intergenic	40.0%
!!	CTCCTTTGAAATGTAGCCAA+TGG	+	Chr2:65767266-65767285	MsG0280010177.01.T01:intron	40.0%
!!	TTGTCCTCCAAAAGCATCAA+TGG	+	Chr2:65767310-65767329	MsG0280010177.01.T01:intron	40.0%
	ACACTCTGTGGACAAGGATT+TGG	+	Chr2:65768281-65768300	MsG0280010177.01.T01:intron	45.0%
	ATGTATGTGTGGGAACCAAG+TGG	+	Chr2:65770675-65770694	MsG0280010177.01.T01:three_prime_UTR	45.0%
	ATTTCCAATGAATTGGGGGC+AGG	+	Chr2:65769723-65769742	MsG0280010177.01.T01:CDS	45.0%
	CAATGTCTGGTGATTGGTGA+AGG	-	Chr2:65768419-65768438	None:intergenic	45.0%
	CACAATCTGTAGTATGCCAC+TGG	+	Chr2:65768526-65768545	MsG0280010177.01.T01:intron	45.0%
	CATACGTGGAAATGGTCTTC+AGG	-	Chr2:65767649-65767668	None:intergenic	45.0%
	CTAGAAACACTCTGTGGACA+AGG	+	Chr2:65768275-65768294	MsG0280010177.01.T01:intron	45.0%
	GAAGCTGTTCTAGCAATGTC+TGG	-	Chr2:65768432-65768451	None:intergenic	45.0%
	GCCTTGCTCTTCTTTGTCTT+AGG	+	Chr2:65768235-65768254	MsG0280010177.01.T01:intron	45.0%
	GTGGCCTAGTGTGTCAAATT+GGG	+	Chr2:65770541-65770560	MsG0280010177.01.T01:CDS	45.0%
	TACCATACCATTCCTGCAGA+AGG	-	Chr2:65768779-65768798	None:intergenic	45.0%
	TGGCCTAGTGTGTCAAATTG+GGG	+	Chr2:65770542-65770561	MsG0280010177.01.T01:CDS	45.0%
	TGTGGCCTAGTGTGTCAAAT+TGG	+	Chr2:65770540-65770559	MsG0280010177.01.T01:CDS	45.0%
	TTCCTTCTGCAGGAATGGTA+TGG	+	Chr2:65768774-65768793	MsG0280010177.01.T01:intron	45.0%
!	AAACTAGCTCTTCCTTCTGC+AGG	+	Chr2:65768764-65768783	MsG0280010177.01.T01:intron	45.0%
!	GACAGGTTTGAGTTCATAGG+TGG	+	Chr2:65768609-65768628	MsG0280010177.01.T01:intron	45.0%
!	GGCCATTGGCTACATTTCAA+AGG	-	Chr2:65767271-65767290	None:intergenic	45.0%
!	TCTGTCCAAGGTGTCTTATC+AGG	+	Chr2:65769933-65769952	MsG0280010177.01.T01:CDS	45.0%
!!	GCGTCTTGCAAAACTTGTTG+AGG	+	Chr2:65768493-65768512	MsG0280010177.01.T01:intron	45.0%
!!	GGGCACTCTTACTTTTGACA+AGG	+	Chr2:65770562-65770581	MsG0280010177.01.T01:CDS	45.0%
!!	GGTGCTACTCTTGCTAATTC+TGG	+	Chr2:65766004-65766023	MsG0280010177.01.T01:CDS	45.0%
!!	TGGACAAGGATTTGGTGCAA+AGG	+	Chr2:65768289-65768308	MsG0280010177.01.T01:intron	45.0%
!!	AAAACAAATAAAAAATTATA+AGG	-	Chr2:65767838-65767857	None:intergenic	5.0%
!!	AATATTTATTAAAATACTAA+AGG	-	Chr2:65766209-65766228	None:intergenic	5.0%
!!!	TTATAAAATTTTATCTAAAT+TGG	-	Chr2:65767393-65767412	None:intergenic	5.0%
	AACTAAAGCTACCGGGGCAT+TGG	-	Chr2:65768919-65768938	None:intergenic	50.0%
	AGCTCTTCCTTCTGCAGGAA+TGG	+	Chr2:65768769-65768788	MsG0280010177.01.T01:intron	50.0%
	AGTGCAGCTGCAAGGTTAGT+TGG	+	Chr2:65769562-65769581	MsG0280010177.01.T01:intron	50.0%
	CAGCTAACTGAAGCTCACCA+AGG	-	Chr2:65765985-65766004	None:intergenic	50.0%
	CAGTTGAGTTACCAATGCCC+CGG	+	Chr2:65768905-65768924	MsG0280010177.01.T01:CDS	50.0%
	CATAATGCAGGGGTGGTTAG+AGG	+	Chr2:65770040-65770059	MsG0280010177.01.T01:intron	50.0%
	CTAAAGCTCTCTGTCCTCCT+TGG	+	Chr2:65769783-65769802	MsG0280010177.01.T01:CDS	50.0%
	CTTGGTGAGCTTCAGTTAGC+TGG	+	Chr2:65765983-65766002	MsG0280010177.01.T01:CDS	50.0%
	GGTGCACTAGAAACACTCTG+TGG	+	Chr2:65768269-65768288	MsG0280010177.01.T01:intron	50.0%
	GTGCCCCAATTTGACACACT+AGG	-	Chr2:65770548-65770567	None:intergenic	50.0%
	TGTCACTGGCGTAGCAGTTA+TGG	+	Chr2:65766032-65766051	MsG0280010177.01.T01:intron	50.0%
	TGTCTTGCATAATGCAGGGG+TGG	+	Chr2:65770033-65770052	MsG0280010177.01.T01:intron	50.0%
!	ATGGTCTTAGTGCAGCTGCA+AGG	+	Chr2:65769554-65769573	MsG0280010177.01.T01:CDS	50.0%
!	GTCATGCTTGTTGGTCACCT+TGG	+	Chr2:65765965-65765984	MsG0280010177.01.T01:CDS	50.0%
	AATGCAGGGGTGGTTAGAGG+AGG	+	Chr2:65770043-65770062	MsG0280010177.01.T01:intron	55.0%
	AGGGGTGGTTAGAGGAGGAT+GGG	+	Chr2:65770048-65770067	MsG0280010177.01.T01:intron	55.0%
	ATGGCCTGCCCCCAATTCAT+TGG	-	Chr2:65769730-65769749	None:intergenic	55.0%
	GTGGTTAGAGGAGGATGGGT+TGG	+	Chr2:65770052-65770071	MsG0280010177.01.T01:intron	55.0%
	TCATGATGTCCGGCTCCACT+TGG	+	Chr2:65766778-65766797	MsG0280010177.01.T01:intron	55.0%
!!	CGGGGCATTGGTAACTCAAC+TGG	-	Chr2:65768907-65768926	None:intergenic	55.0%
	CAGGGGTGGTTAGAGGAGGA+TGG	+	Chr2:65770047-65770066	MsG0280010177.01.T01:intron	60.0%
!!	GAGGATGGGTTGGCAGCACT+TGG	+	Chr2:65770062-65770081	MsG0280010177.01.T01:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65765848	65770869	65765848	ID=MsG0280010177.01;Name=MsG0280010177.01
Chr2	mRNA	65765848	65770869	65765848	ID=MsG0280010177.01.T01;Parent=MsG0280010177.01;Name=MsG0280010177.01.T01;_AED=0.31;_eAED=0.31;_QI=0\|0.2\|0.5\|0.83\|1\|1\|6\|268\|259
Chr2	exon	65765848	65766041	65765848	ID=MsG0280010177.01.T01:exon:5141;Parent=MsG0280010177.01.T01
Chr2	exon	65768614	65768744	65768614	ID=MsG0280010177.01.T01:exon:5142;Parent=MsG0280010177.01.T01
Chr2	exon	65768851	65768926	65768851	ID=MsG0280010177.01.T01:exon:5143;Parent=MsG0280010177.01.T01
Chr2	exon	65769519	65769575	65769519	ID=MsG0280010177.01.T01:exon:5144;Parent=MsG0280010177.01.T01
Chr2	exon	65769716	65769954	65769716	ID=MsG0280010177.01.T01:exon:5145;Parent=MsG0280010177.01.T01
Chr2	exon	65770519	65770869	65770519	ID=MsG0280010177.01.T01:exon:5146;Parent=MsG0280010177.01.T01
Chr2	CDS	65765848	65766041	65765848	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	CDS	65768614	65768744	65768614	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	CDS	65768851	65768926	65768851	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	CDS	65769519	65769575	65769519	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	CDS	65769716	65769954	65769716	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	CDS	65770519	65770601	65770519	ID=MsG0280010177.01.T01:cds;Parent=MsG0280010177.01.T01
Chr2	three_prime_UTR	65770602	65770869	65770602	ID=MsG0280010177.01.T01:three_prime_utr;Parent=MsG0280010177.01.T01

Gene Sequence

>MsG0280010177.01.T01

ATGGAGGACAAATGGTGGAACAAAATCTTGGACATGGAGGAAGCCAAAACAACAACTCATGTTTTCACTACCAATGATTTTTACAAACTTATTTATTACTTGATTACTTTGGTTTCTGTCATGCTTGTTGGTCACCTTGGTGAGCTTCAGTTAGCTGGTGCTACTCTTGCTAATTCTGGTTTAGTGTCACTGGCGTTTGAGTTCATAGGTGGACCAGTTGCAACTTCTATTTCACTATGGATATCTATGATGTTGGTAGGTTTTTATGTCTTGTATGCAAAGAAGTTGAGAATACATGGAGAGGATTTTCAATGGAGTCATTTCAAAAACAAAGTTGCTAAATTATTAGGCCGGTTTACTTTGAGCAACATTTTCAAACCAGTTGAGTTACCAATGCCCCGTGAAAACACAGAATTCATTGCTTACATGATCACTTATGGTCTTAGTGCAGCTGCAAGCACAAGGATTTCCAATGAATTGGGGGCAGGCCATCCTGAAAGAGCTAAACATGCAATGAGAGTCACTCTAAAGCTCTCTGTCCTCCTTGGATTCTGTTTTGTTTTGATGCTTTTGTTTGGTCATGATATATGGATTCAAATGTTTAGTGATAGTCCTATTATCAAAAAGGAGTTTGCTTCAATAACACCTTTGCTTGCTATTTCCATACTACTAGATTCTGTCCAAGGTGTCTTATCAGGGTTTATGGATTGGTCTAATTTGTGGCCTAGTGTGTCAAATTGGGGCACTCTTACTTTTGACAAGGCATGCCAAATGGACTAA

Protein sequence

>MsG0280010177.01.T01

MEDKWWNKILDMEEAKTTTHVFTTNDFYKLIYYLITLVSVMLVGHLGELQLAGATLANSGLVSLAFEFIGGPVATSISLWISMMLVGFYVLYAKKLRIHGEDFQWSHFKNKVAKLLGRFTLSNIFKPVELPMPRENTEFIAYMITYGLSAAASTRISNELGAGHPERAKHAMRVTLKLSVLLGFCFVLMLLFGHDIWIQMFSDSPIIKKEFASITPLLAISILLDSVQGVLSGFMDWSNLWPSVSNWGTLTFDKACQMD*