AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022207.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAGGAGGTGGTGGCAG+TGG 0.345303 4:-69699034 MsG0480022207.01.T01:CDS
GTGTGGATGGATTTAGTATT+GGG 0.354043 4:-69698974 MsG0480022207.01.T01:CDS
GGGGATGATGGAGGAGGTGG+TGG 0.356511 4:-69699040 MsG0480022207.01.T01:CDS
GTTGGGTTGGGAGTTGGTGC+TGG 0.362932 4:-69698939 MsG0480022207.01.T01:CDS
AGGTGGTGGCAGTGGTGGAC+GGG 0.368752 4:-69699026 MsG0480022207.01.T01:CDS
TGTGGGGATGATGGAGGAGG+TGG 0.381411 4:-69699043 MsG0480022207.01.T01:CDS
AAGTCGGAGACGGTTGGGTT+GGG 0.388880 4:-69698951 MsG0480022207.01.T01:CDS
GAGACGGTTGGGTTGGGAGT+TGG 0.391170 4:-69698945 MsG0480022207.01.T01:CDS
GGTGGTGGCAGTGGTGGACG+GGG 0.392590 4:-69699025 MsG0480022207.01.T01:CDS
AAAGGCAAGGGCTTGGGAAC+AGG 0.399657 4:-69698869 MsG0480022207.01.T01:CDS
GTGGAACAAAGGCAAGGGCT+TGG 0.407313 4:-69698876 MsG0480022207.01.T01:CDS
GGAGGAGGTGGTGGCAGTGG+TGG 0.427114 4:-69699031 MsG0480022207.01.T01:CDS
GAGGTGGTGGCAGTGGTGGA+CGG 0.428340 4:-69699027 MsG0480022207.01.T01:CDS
TGGAACAAAGGCAAGGGCTT+GGG 0.428739 4:-69698875 MsG0480022207.01.T01:CDS
CCGAGGTCGCCATCGCCTCC+CGG 0.432302 4:-69698999 MsG0480022207.01.T01:CDS
TGCTCAGATGATGGCAAAGA+TGG 0.442413 4:-69698900 MsG0480022207.01.T01:CDS
GAGAAGGCGTGTGGGGATGA+TGG 0.443412 4:-69699052 MsG0480022207.01.T01:CDS
GGGTTGGGAGTTGGTGCTGG+TGG 0.448431 4:-69698936 MsG0480022207.01.T01:CDS
TCGCCTCCCGGGAGTGTGGA+TGG 0.452371 4:-69698987 MsG0480022207.01.T01:CDS
GAAGTCGGAGACGGTTGGGT+TGG 0.461972 4:-69698952 MsG0480022207.01.T01:CDS
AGGGATTACAACTCCGTTGA+TGG 0.463810 4:-69698842 MsG0480022207.01.T01:CDS
AGTGTGGATGGATTTAGTAT+TGG 0.474626 4:-69698975 MsG0480022207.01.T01:CDS
TTGGGAAGTCGGAGACGGTT+GGG 0.475282 4:-69698956 MsG0480022207.01.T01:CDS
GTGGTGGCAGTGGTGGACGG+GGG 0.490811 4:-69699024 MsG0480022207.01.T01:CDS
TGGATTTAGTATTGGGAAGT+CGG 0.501213 4:-69698967 MsG0480022207.01.T01:CDS
ACTAAATCCATCCACACTCC+CGG 0.501604 4:+69698980 None:intergenic
CGAGGTCGCCATCGCCTCCC+GGG 0.503095 4:-69698998 MsG0480022207.01.T01:CDS
TCCACACTCCCGGGAGGCGA+TGG 0.508661 4:+69698990 None:intergenic
AGGGCTTGGGAACAGGAGCA+AGG 0.511591 4:-69698862 MsG0480022207.01.T01:CDS
GCGTGTGGGGATGATGGAGG+AGG 0.519021 4:-69699046 MsG0480022207.01.T01:CDS
ATTGGGAAGTCGGAGACGGT+TGG 0.535983 4:-69698957 MsG0480022207.01.T01:CDS
TACTGCTGCTGCTCAGATGA+TGG 0.540524 4:-69698909 MsG0480022207.01.T01:CDS
AAGGCGTGTGGGGATGATGG+AGG 0.545116 4:-69699049 MsG0480022207.01.T01:CDS
CTAAATCCATCCACACTCCC+GGG 0.550046 4:+69698981 None:intergenic
CCGGGAGGCGATGGCGACCT+CGG 0.552926 4:+69698999 None:intergenic
GCTCAGATGATGGCAAAGAT+GGG 0.559134 4:-69698899 MsG0480022207.01.T01:CDS
GCCATCGCCTCCCGGGAGTG+TGG 0.566180 4:-69698991 MsG0480022207.01.T01:CDS
CCGGTGAGGAAGCATGGAGA+AGG 0.570595 4:-69699068 None:intergenic
GGGCTTGGGAACAGGAGCAA+GGG 0.572292 4:-69698861 MsG0480022207.01.T01:CDS
GCAAAGATGGGGTGGAACAA+AGG 0.577039 4:-69698887 MsG0480022207.01.T01:CDS
TAGTATTGGGAAGTCGGAGA+CGG 0.588760 4:-69698961 MsG0480022207.01.T01:CDS
GAAGCATGGAGAAGGCGTGT+GGG 0.595489 4:-69699060 None:intergenic
GATGGGGTGGAACAAAGGCA+AGG 0.595503 4:-69698882 MsG0480022207.01.T01:CDS
GGAAGCATGGAGAAGGCGTG+TGG 0.600681 4:-69699061 None:intergenic
AGATGATGGCAAAGATGGGG+TGG 0.606439 4:-69698895 MsG0480022207.01.T01:CDS
AAGCATGGAGAAGGCGTGTG+GGG 0.635401 4:-69699059 None:intergenic
ATGGGGTGGAACAAAGGCAA+GGG 0.665903 4:-69698881 MsG0480022207.01.T01:CDS
CTCAGATGATGGCAAAGATG+GGG 0.693277 4:-69698898 MsG0480022207.01.T01:CDS
AATCCATCCACACTCCCGGG+AGG 0.744351 4:+69698984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTGTGGATGGATTTAGTAT+TGG - Chr4:69698906-69698925 MsG0480022207.01.T01:CDS 35.0%
! GTGTGGATGGATTTAGTATT+GGG - Chr4:69698907-69698926 MsG0480022207.01.T01:CDS 35.0%
! TGGATTTAGTATTGGGAAGT+CGG - Chr4:69698914-69698933 MsG0480022207.01.T01:CDS 35.0%
ACTAAATCCATCCACACTCC+CGG + Chr4:69698904-69698923 None:intergenic 45.0%
AGGGATTACAACTCCGTTGA+TGG - Chr4:69699039-69699058 MsG0480022207.01.T01:CDS 45.0%
! CTCAGATGATGGCAAAGATG+GGG - Chr4:69698983-69699002 MsG0480022207.01.T01:CDS 45.0%
! GCTCAGATGATGGCAAAGAT+GGG - Chr4:69698982-69699001 MsG0480022207.01.T01:CDS 45.0%
! TAGTATTGGGAAGTCGGAGA+CGG - Chr4:69698920-69698939 MsG0480022207.01.T01:CDS 45.0%
! TCGGTTTTCTTCGCCATCAA+CGG + Chr4:69699055-69699074 None:intergenic 45.0%
! TGCTCAGATGATGGCAAAGA+TGG - Chr4:69698981-69699000 MsG0480022207.01.T01:CDS 45.0%
ATGGGGTGGAACAAAGGCAA+GGG - Chr4:69699000-69699019 MsG0480022207.01.T01:CDS 50.0%
CTAAATCCATCCACACTCCC+GGG + Chr4:69698903-69698922 None:intergenic 50.0%
GCAAAGATGGGGTGGAACAA+AGG - Chr4:69698994-69699013 MsG0480022207.01.T01:CDS 50.0%
TACTGCTGCTGCTCAGATGA+TGG - Chr4:69698972-69698991 MsG0480022207.01.T01:CDS 50.0%
TGGAACAAAGGCAAGGGCTT+GGG - Chr4:69699006-69699025 MsG0480022207.01.T01:CDS 50.0%
! AGATGATGGCAAAGATGGGG+TGG - Chr4:69698986-69699005 MsG0480022207.01.T01:CDS 50.0%
AAAGGCAAGGGCTTGGGAAC+AGG - Chr4:69699012-69699031 MsG0480022207.01.T01:CDS 55.0%
AAGTCGGAGACGGTTGGGTT+GGG - Chr4:69698930-69698949 MsG0480022207.01.T01:CDS 55.0%
ATTGGGAAGTCGGAGACGGT+TGG - Chr4:69698924-69698943 MsG0480022207.01.T01:CDS 55.0%
GATGGGGTGGAACAAAGGCA+AGG - Chr4:69698999-69699018 MsG0480022207.01.T01:CDS 55.0%
GTGGAACAAAGGCAAGGGCT+TGG - Chr4:69699005-69699024 MsG0480022207.01.T01:CDS 55.0%
TTGGGAAGTCGGAGACGGTT+GGG - Chr4:69698925-69698944 MsG0480022207.01.T01:CDS 55.0%
AATCCATCCACACTCCCGGG+AGG + Chr4:69698900-69698919 None:intergenic 60.0%
AGGGCTTGGGAACAGGAGCA+AGG - Chr4:69699019-69699038 MsG0480022207.01.T01:CDS 60.0%
GAAGTCGGAGACGGTTGGGT+TGG - Chr4:69698929-69698948 MsG0480022207.01.T01:CDS 60.0%
GGGCTTGGGAACAGGAGCAA+GGG - Chr4:69699020-69699039 MsG0480022207.01.T01:CDS 60.0%
! AAGGCGTGTGGGGATGATGG+AGG - Chr4:69698832-69698851 MsG0480022207.01.T01:CDS 60.0%
! GAGAAGGCGTGTGGGGATGA+TGG - Chr4:69698829-69698848 MsG0480022207.01.T01:CDS 60.0%
!! GAGACGGTTGGGTTGGGAGT+TGG - Chr4:69698936-69698955 MsG0480022207.01.T01:CDS 60.0%
!! GTTGGGTTGGGAGTTGGTGC+TGG - Chr4:69698942-69698961 MsG0480022207.01.T01:CDS 60.0%
!! TGTGGGGATGATGGAGGAGG+TGG - Chr4:69698838-69698857 MsG0480022207.01.T01:CDS 60.0%
! GAGGTGGTGGCAGTGGTGGA+CGG - Chr4:69698854-69698873 MsG0480022207.01.T01:CDS 65.0%
! GATGGAGGAGGTGGTGGCAG+TGG - Chr4:69698847-69698866 MsG0480022207.01.T01:CDS 65.0%
!! AGGTGGTGGCAGTGGTGGAC+GGG - Chr4:69698855-69698874 MsG0480022207.01.T01:CDS 65.0%
!! GCGTGTGGGGATGATGGAGG+AGG - Chr4:69698835-69698854 MsG0480022207.01.T01:CDS 65.0%
!! GGGGATGATGGAGGAGGTGG+TGG - Chr4:69698841-69698860 MsG0480022207.01.T01:CDS 65.0%
!! GGGTTGGGAGTTGGTGCTGG+TGG - Chr4:69698945-69698964 MsG0480022207.01.T01:CDS 65.0%
TCCACACTCCCGGGAGGCGA+TGG + Chr4:69698894-69698913 None:intergenic 70.0%
! GGAGGAGGTGGTGGCAGTGG+TGG - Chr4:69698850-69698869 MsG0480022207.01.T01:CDS 70.0%
! TCGCCTCCCGGGAGTGTGGA+TGG - Chr4:69698894-69698913 MsG0480022207.01.T01:CDS 70.0%
!! GGTGGTGGCAGTGGTGGACG+GGG - Chr4:69698856-69698875 MsG0480022207.01.T01:CDS 70.0%
!! GTGGTGGCAGTGGTGGACGG+GGG - Chr4:69698857-69698876 MsG0480022207.01.T01:CDS 70.0%
CCGAGGTCGCCATCGCCTCC+CGG - Chr4:69698882-69698901 MsG0480022207.01.T01:CDS 75.0%
CCGGGAGGCGATGGCGACCT+CGG + Chr4:69698885-69698904 None:intergenic 75.0%
CGAGGTCGCCATCGCCTCCC+GGG - Chr4:69698883-69698902 MsG0480022207.01.T01:CDS 75.0%
GCCATCGCCTCCCGGGAGTG+TGG - Chr4:69698890-69698909 MsG0480022207.01.T01:CDS 75.0%
!! AGTGGTGGACGGGGGTGCCG+AGG - Chr4:69698865-69698884 MsG0480022207.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 69698826 69699077 69698826 ID=MsG0480022207.01;Name=MsG0480022207.01
Chr4 mRNA 69698826 69699077 69698826 ID=MsG0480022207.01.T01;Parent=MsG0480022207.01;Name=MsG0480022207.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|83
Chr4 exon 69698826 69699077 69698826 ID=MsG0480022207.01.T01:exon:55564;Parent=MsG0480022207.01.T01
Chr4 CDS 69698826 69699077 69698826 ID=MsG0480022207.01.T01:cds;Parent=MsG0480022207.01.T01
Gene Sequence

>MsG0480022207.01.T01

ATGGAGAAGGCGTGTGGGGATGATGGAGGAGGTGGTGGCAGTGGTGGACGGGGGTGCCGAGGTCGCCATCGCCTCCCGGGAGTGTGGATGGATTTAGTATTGGGAAGTCGGAGACGGTTGGGTTGGGAGTTGGTGCTGGTGGCAGATACTGCTGCTGCTCAGATGATGGCAAAGATGGGGTGGAACAAAGGCAAGGGCTTGGGAACAGGAGCAAGGGATTACAACTCCGTTGATGGCGAAGAAAACCGATAG

Protein sequence

>MsG0480022207.01.T01

MEKACGDDGGGGGSGGRGCRGRHRLPGVWMDLVLGSRRRLGWELVLVADTAAAQMMAKMGWNKGKGLGTGARDYNSVDGEENR*