AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008936.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008936.01.T01 MTR_0066s0040 95.402 87 3 1 1 87 1 86 3.23e-56 169
MsG0280008936.01.T01 MTR_4g115580 56.061 66 29 0 4 69 38 103 6.92e-23 90.1
MsG0280008936.01.T01 MTR_5g015270 52.941 68 32 0 7 74 28 95 6.67e-20 82.0
MsG0280008936.01.T01 MTR_5g015270 52.941 68 32 0 7 74 28 95 7.58e-20 82.0
MsG0280008936.01.T01 MTR_3g103240 58.929 56 23 0 7 62 32 87 1.78e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008936.01.T01 AT4G30850 51.190 84 35 1 1 78 19 102 3.10e-25 96.3
MsG0280008936.01.T01 AT4G30850 51.190 84 35 1 1 78 19 102 3.10e-25 96.3
MsG0280008936.01.T01 AT2G24150 67.241 58 19 0 12 69 33 90 2.66e-24 94.0
MsG0280008936.01.T01 AT5G20270 57.353 68 29 0 2 69 45 112 3.87e-24 93.2
MsG0280008936.01.T01 AT4G38320 50.000 78 34 1 2 74 23 100 6.71e-20 82.0
MsG0280008936.01.T01 AT4G37680 50.000 78 34 1 2 74 23 100 9.76e-20 81.6
MsG0280008936.01.T01 AT4G37680 50.000 78 34 1 2 74 23 100 9.76e-20 81.6
MsG0280008936.01.T01 AT4G37680 50.000 78 34 1 2 74 23 100 9.76e-20 81.6
MsG0280008936.01.T01 AT4G37680 50.000 78 34 1 2 74 28 105 1.07e-19 81.6
MsG0280008936.01.T01 AT4G37680 50.000 78 34 1 2 74 28 105 1.07e-19 81.6

Find 29 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGTTCTTGTACAGTCATT+TGG 0.300362 2:-41812753 MsG0280008936.01.T01:intron
TTGTTCTTGTACAGTCATTT+GGG 0.334474 2:-41812752 MsG0280008936.01.T01:intron
AAAAGACAACGAGTTCATAT+TGG 0.344022 2:-41812925 MsG0280008936.01.T01:CDS
GCGATGGCGGCGACGGAAGC+TGG 0.345791 2:-41812710 MsG0280008936.01.T01:CDS
AGTTCATATTGGATTATTAC+CGG 0.383152 2:-41812914 MsG0280008936.01.T01:CDS
AAAACGAAACGAAGGAATAA+AGG 0.389374 2:-41813102 None:intergenic
TGGATTATTACCGGTCGAAA+TGG 0.399066 2:-41812905 MsG0280008936.01.T01:CDS
GCGACGGAAGCTGGAGGAGG+AGG 0.408012 2:-41812701 MsG0280008936.01.T01:CDS
ACAACGAAACGCTAAATGTT+TGG 0.429326 2:-41812845 MsG0280008936.01.T01:CDS
GGTGAAATACGAGGAATTGC+CGG 0.439654 2:-41812955 MsG0280008936.01.T01:CDS
ACGGAAGCTGGAGGAGGAGG+AGG 0.442291 2:-41812698 MsG0280008936.01.T01:CDS
GGAATAAAGGTGCAAGCGAC+GGG 0.459408 2:-41813089 MsG0280008936.01.T01:five_prime_UTR
CGAATATGAAGGGTAATTAT+CGG 0.467352 2:-41812998 MsG0280008936.01.T01:exon
CCGGTCGAAATGGTCGGTTA+AGG 0.472175 2:-41812895 MsG0280008936.01.T01:CDS
GCGGCGACGGAAGCTGGAGG+AGG 0.486075 2:-41812704 MsG0280008936.01.T01:CDS
TTTAATATTTGCAGCGATGG+CGG 0.490595 2:-41812723 MsG0280008936.01.T01:CDS
GAAGCTGGAGGAGGAGGAGG+TGG 0.496794 2:-41812695 MsG0280008936.01.T01:CDS
AGGAATAAAGGTGCAAGCGA+CGG 0.501490 2:-41813090 MsG0280008936.01.T01:five_prime_UTR
ATTTGCAGCGATGGCGGCGA+CGG 0.523006 2:-41812717 MsG0280008936.01.T01:CDS
GCGGAAGAGAACGTCGCGAT+TGG 0.526186 2:-41812976 MsG0280008936.01.T01:CDS
GATAATATAAATTTAATCGC+CGG 0.534759 2:-41813048 MsG0280008936.01.T01:five_prime_UTR
TAATTACCCTTCATATTCGC+CGG 0.541667 2:+41813002 None:intergenic
TTATTACCGGTCGAAATGGT+CGG 0.550345 2:-41812901 MsG0280008936.01.T01:CDS
TTCTTGTACAGTCATTTGGG+AGG 0.573521 2:-41812749 MsG0280008936.01.T01:intron
TAAAGGTGCAAGCGACGGGA+AGG 0.593094 2:-41813085 MsG0280008936.01.T01:five_prime_UTR
CCTTAACCGACCATTTCGAC+CGG 0.620356 2:+41812895 None:intergenic
ATGGCGGCGACGGAAGCTGG+AGG 0.636720 2:-41812707 MsG0280008936.01.T01:CDS
ATATGAAGGGTAATTATCGG+CGG 0.702822 2:-41812995 MsG0280008936.01.T01:CDS
GTCGCGATTGGTGAAATACG+AGG 0.714635 2:-41812964 MsG0280008936.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTATTGCTAAAATCTTAA+CGG + Chr2:41812953-41812972 None:intergenic 20.0%
!! GATAATATAAATTTAATCGC+CGG - Chr2:41812710-41812729 MsG0280008936.01.T01:CDS 20.0%
! AGTTCATATTGGATTATTAC+CGG - Chr2:41812844-41812863 MsG0280008936.01.T01:CDS 25.0%
!! CGAAAAAAGAAAAGATTTTC+CGG - Chr2:41812737-41812756 MsG0280008936.01.T01:CDS 25.0%
!!! TCTTTTCTTTTTTCGTTTTC+CGG + Chr2:41812732-41812751 None:intergenic 25.0%
AAAAGACAACGAGTTCATAT+TGG - Chr2:41812833-41812852 MsG0280008936.01.T01:intron 30.0%
CGAATATGAAGGGTAATTAT+CGG - Chr2:41812760-41812779 MsG0280008936.01.T01:intron 30.0%
TTGTTCTTGTACAGTCATTT+GGG - Chr2:41813006-41813025 MsG0280008936.01.T01:exon 30.0%
! CTTTTTAATATTTGCAGCGA+TGG - Chr2:41813032-41813051 MsG0280008936.01.T01:five_prime_UTR 30.0%
! GTTTTCTGTCTAATATCTAC+AGG - Chr2:41813085-41813104 MsG0280008936.01.T01:five_prime_UTR 30.0%
! TCGTTGTCTTTTAGATATTC+CGG + Chr2:41812825-41812844 None:intergenic 30.0%
AACAACAACGATGTCAAACA+TGG + Chr2:41812988-41813007 None:intergenic 35.0%
ACAACGAAACGCTAAATGTT+TGG - Chr2:41812913-41812932 MsG0280008936.01.T01:CDS 35.0%
ATATGAAGGGTAATTATCGG+CGG - Chr2:41812763-41812782 MsG0280008936.01.T01:intron 35.0%
GTTGTTCTTGTACAGTCATT+TGG - Chr2:41813005-41813024 MsG0280008936.01.T01:exon 35.0%
TAATTACCCTTCATATTCGC+CGG + Chr2:41812759-41812778 None:intergenic 35.0%
TTTAATATTTGCAGCGATGG+CGG - Chr2:41813035-41813054 MsG0280008936.01.T01:five_prime_UTR 35.0%
TGGATTATTACCGGTCGAAA+TGG - Chr2:41812853-41812872 MsG0280008936.01.T01:CDS 40.0%
TTATTACCGGTCGAAATGGT+CGG - Chr2:41812857-41812876 MsG0280008936.01.T01:CDS 40.0%
TTCTTGTACAGTCATTTGGG+AGG - Chr2:41813009-41813028 MsG0280008936.01.T01:exon 40.0%
! AGATTTTCCGGCGAATATGA+AGG - Chr2:41812749-41812768 MsG0280008936.01.T01:intron 40.0%
! GATTTTCCGGCGAATATGAA+GGG - Chr2:41812750-41812769 MsG0280008936.01.T01:intron 40.0%
GGTGAAATACGAGGAATTGC+CGG - Chr2:41812803-41812822 MsG0280008936.01.T01:intron 45.0%
! AGGAATAAAGGTGCAAGCGA+CGG - Chr2:41812668-41812687 MsG0280008936.01.T01:CDS 45.0%
CCTTAACCGACCATTTCGAC+CGG + Chr2:41812866-41812885 None:intergenic 50.0%
GTCGCGATTGGTGAAATACG+AGG - Chr2:41812794-41812813 MsG0280008936.01.T01:intron 50.0%
! CTTTGTTCAGCGTTTTCGCG+TGG - Chr2:41812889-41812908 MsG0280008936.01.T01:CDS 50.0%
! GGAATAAAGGTGCAAGCGAC+GGG - Chr2:41812669-41812688 MsG0280008936.01.T01:CDS 50.0%
CCGGTCGAAATGGTCGGTTA+AGG - Chr2:41812863-41812882 MsG0280008936.01.T01:CDS 55.0%
! TAAAGGTGCAAGCGACGGGA+AGG - Chr2:41812673-41812692 MsG0280008936.01.T01:CDS 55.0%
GCGGAAGAGAACGTCGCGAT+TGG - Chr2:41812782-41812801 MsG0280008936.01.T01:intron 60.0%
! ATTTGCAGCGATGGCGGCGA+CGG - Chr2:41813041-41813060 MsG0280008936.01.T01:five_prime_UTR 60.0%
ACGGAAGCTGGAGGAGGAGG+AGG - Chr2:41813060-41813079 MsG0280008936.01.T01:five_prime_UTR 65.0%
GAAGCTGGAGGAGGAGGAGG+TGG - Chr2:41813063-41813082 MsG0280008936.01.T01:five_prime_UTR 65.0%
ATGGCGGCGACGGAAGCTGG+AGG - Chr2:41813051-41813070 MsG0280008936.01.T01:five_prime_UTR 70.0%
GCGACGGAAGCTGGAGGAGG+AGG - Chr2:41813057-41813076 MsG0280008936.01.T01:five_prime_UTR 70.0%
GCGGCGACGGAAGCTGGAGG+AGG - Chr2:41813054-41813073 MsG0280008936.01.T01:five_prime_UTR 75.0%
! GCGATGGCGGCGACGGAAGC+TGG - Chr2:41813048-41813067 MsG0280008936.01.T01:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 41812667 41813113 41812667 ID=MsG0280008936.01;Name=MsG0280008936.01
Chr2 mRNA 41812667 41813113 41812667 ID=MsG0280008936.01.T01;Parent=MsG0280008936.01;Name=MsG0280008936.01.T01;_AED=0.49;_eAED=0.50;_QI=98|1|0.5|1|0|0|2|0|88
Chr2 exon 41812843 41813113 41812843 ID=MsG0280008936.01.T01:exon:29594;Parent=MsG0280008936.01.T01
Chr2 exon 41812667 41812760 41812667 ID=MsG0280008936.01.T01:exon:29595;Parent=MsG0280008936.01.T01
Chr2 five_prime_UTR 41813016 41813113 41813016 ID=MsG0280008936.01.T01:five_prime_utr;Parent=MsG0280008936.01.T01
Chr2 CDS 41812843 41813015 41812843 ID=MsG0280008936.01.T01:cds;Parent=MsG0280008936.01.T01
Chr2 CDS 41812667 41812760 41812667 ID=MsG0280008936.01.T01:cds;Parent=MsG0280008936.01.T01
Gene Sequence

>MsG0280008936.01.T01

ATGAAGGGTAATTATCGGCGGAAGAGAACGTCGCGATTGGTGAAATACGAGGAATTGCCGGAATATCTAAAAGACAACGAGTTCATATTGGATTATTACCGGTCGAAATGGTCGGTTAAGGATGCTTTGTTCAGCGTTTTCGCGTGGCACAACGAAACGCTAAATGTTTGGACTCATTTGGGAGGCTTTTTAATATTTGCAGCGATGGCGGCGACGGAAGCTGGAGGAGGAGGAGGTGGTTTTCTGTCTAATATCTACAGGTATTGA

Protein sequence

>MsG0280008936.01.T01

MKGNYRRKRTSRLVKYEELPEYLKDNEFILDYYRSKWSVKDALFSVFAWHNETLNVWTHLGGFLIFAAMAATEAGGGGGGFLSNIYRY*