AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006368.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006368.01.T01 MTR_2g007010 96.296 81 3 0 1 81 239 319 5.04e-51 171
MsG0280006368.01.T01 MTR_2g007010 36.585 82 51 1 2 82 530 611 4.01e-11 58.2
MsG0280006368.01.T01 MTR_3g023900 66.265 83 26 1 1 81 235 317 2.01e-29 110
MsG0280006368.01.T01 MTR_3g023900 39.506 81 48 1 2 81 533 613 1.04e-11 59.7
MsG0280006368.01.T01 MTR_3g023890 56.322 87 34 2 1 83 93 179 8.43e-23 91.3
MsG0280006368.01.T01 MTR_3g023890 39.744 78 46 1 2 78 387 464 5.69e-11 57.4
MsG0280006368.01.T01 MTR_8g099605 44.304 79 44 0 2 80 165 243 1.07e-18 79.3
MsG0280006368.01.T01 MTR_8g099605 44.304 79 44 0 2 80 406 484 1.08e-18 79.3
MsG0280006368.01.T01 MTR_7g088850 44.444 81 44 1 2 81 284 364 7.43e-16 71.2
MsG0280006368.01.T01 MTR_7g101870 43.210 81 45 1 2 81 208 288 5.24e-15 68.9
MsG0280006368.01.T01 MTR_7g101870 38.272 81 49 1 2 81 481 561 4.38e-11 57.8
MsG0280006368.01.T01 MTR_1g063380 43.210 81 45 1 2 81 207 287 5.24e-15 68.9
MsG0280006368.01.T01 MTR_1g063380 38.272 81 49 1 2 81 480 560 2.64e-11 58.5
MsG0280006368.01.T01 MTR_2g095320 43.750 80 42 2 2 81 155 231 1.96e-14 67.4
MsG0280006368.01.T01 MTR_2g095320 38.272 81 49 1 2 81 423 503 2.72e-11 58.5
MsG0280006368.01.T01 MTR_1g069105 37.179 78 48 1 2 78 190 267 2.47e-13 64.3
MsG0280006368.01.T01 MTR_7g099640 37.179 78 48 1 2 78 189 266 2.52e-13 64.3
MsG0280006368.01.T01 MTR_7g099640 37.179 78 48 1 2 78 189 266 2.55e-13 64.3
MsG0280006368.01.T01 MTR_1g069105 37.179 78 48 1 2 78 190 267 2.55e-13 64.3
MsG0280006368.01.T01 MTR_3g096000 44.304 79 44 0 2 80 298 376 8.23e-13 62.8
MsG0280006368.01.T01 MTR_2g095210 42.308 78 44 1 2 78 209 286 1.22e-12 62.4
MsG0280006368.01.T01 MTR_2g095210 39.189 74 44 1 2 74 408 481 3.09e-11 58.2
MsG0280006368.01.T01 MTR_7g078570 43.038 79 45 0 2 80 194 272 1.28e-12 62.4
MsG0280006368.01.T01 MTR_4g094662 41.772 79 46 0 2 80 231 309 1.45e-12 62.0
MsG0280006368.01.T01 MTR_1g101870 45.122 82 43 2 2 81 507 588 1.50e-12 62.0
MsG0280006368.01.T01 MTR_1g101870 45.122 82 43 2 2 81 559 640 1.51e-12 62.0
MsG0280006368.01.T01 MTR_2g438140 41.772 79 46 0 2 80 228 306 1.62e-12 62.0
MsG0280006368.01.T01 MTR_1g099480 43.902 82 44 2 2 81 470 551 1.93e-12 61.6
MsG0280006368.01.T01 MTR_1g099480 43.902 82 44 2 2 81 470 551 1.95e-12 61.6
MsG0280006368.01.T01 MTR_6g016600 42.045 88 44 3 2 83 319 405 2.29e-12 61.6
MsG0280006368.01.T01 MTR_2g438140 41.772 79 46 0 2 80 288 366 2.56e-12 61.2
MsG0280006368.01.T01 MTR_4g064350 41.772 79 46 0 2 80 322 400 2.78e-12 61.2
MsG0280006368.01.T01 MTR_6g033740 46.053 76 36 2 2 74 502 575 3.76e-12 60.8
MsG0280006368.01.T01 MTR_1g099470 45.333 75 39 2 2 74 436 510 4.33e-12 60.8
MsG0280006368.01.T01 MTR_3g112260 36.667 90 51 2 2 85 138 227 4.74e-12 60.5
MsG0280006368.01.T01 MTR_3g117230 36.667 90 51 2 2 85 141 230 4.75e-12 60.5
MsG0280006368.01.T01 MTR_3g462930 40.230 87 47 1 2 83 306 392 7.49e-12 60.1
MsG0280006368.01.T01 MTR_5g069780 40.230 87 47 1 2 83 315 401 9.84e-12 59.7
MsG0280006368.01.T01 MTR_3g062510 39.744 78 46 1 2 78 161 238 1.00e-11 59.7
MsG0280006368.01.T01 MTR_3g062510 39.744 78 46 1 2 78 161 238 1.04e-11 59.7
MsG0280006368.01.T01 MTR_7g096060 40.506 79 47 0 2 80 242 320 1.04e-11 59.7
MsG0280006368.01.T01 MTR_1g095660 37.500 96 49 4 2 87 120 214 1.45e-11 59.3
MsG0280006368.01.T01 MTR_8g469460 40.230 87 47 1 2 83 209 295 2.10e-11 58.9
MsG0280006368.01.T01 MTR_6g059690 39.080 87 48 1 2 83 199 285 2.28e-11 58.5
MsG0280006368.01.T01 MTR_3g104490 45.714 70 38 0 2 71 209 278 2.66e-11 58.5
MsG0280006368.01.T01 MTR_5g009840 44.737 76 37 2 2 74 518 591 2.80e-11 58.5
MsG0280006368.01.T01 MTR_7g088980 41.975 81 46 1 2 81 288 368 4.31e-11 57.8
MsG0280006368.01.T01 MTR_7g088980 41.975 81 46 1 2 81 138 218 4.42e-11 57.8
MsG0280006368.01.T01 MTR_7g088980 41.975 81 46 1 2 81 220 300 4.48e-11 57.8
MsG0280006368.01.T01 MTR_7g088980 41.975 81 46 1 2 81 314 394 4.59e-11 57.8
MsG0280006368.01.T01 MTR_4g094270 40.000 75 44 1 5 78 161 235 4.98e-11 57.8
MsG0280006368.01.T01 MTR_1g083330 36.986 73 45 1 2 73 169 241 5.35e-11 57.4
MsG0280006368.01.T01 MTR_4g033235 36.986 73 45 1 2 73 170 242 5.35e-11 57.4
MsG0280006368.01.T01 MTR_3g115110 40.230 87 47 1 2 83 251 337 6.87e-11 57.4
MsG0280006368.01.T01 MTR_2g101290 40.506 79 47 0 2 80 417 495 9.68e-11 57.0
MsG0280006368.01.T01 MTR_7g088830 38.095 84 45 2 2 81 660 740 9.73e-11 57.0
MsG0280006368.01.T01 MTR_2g101290 40.506 79 47 0 2 80 417 495 9.78e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006368.01.T01 AT3G01610 62.963 81 29 1 2 81 232 312 7.67e-31 114
MsG0280006368.01.T01 AT3G01610 62.963 81 29 1 2 81 232 312 7.67e-31 114
MsG0280006368.01.T01 AT4G23940 43.038 79 45 0 2 80 429 507 5.72e-18 77.4
MsG0280006368.01.T01 AT3G09840 43.210 81 45 1 2 81 206 286 5.49e-15 68.9
MsG0280006368.01.T01 AT3G09840 37.037 81 50 1 2 81 479 559 6.26e-11 57.4
MsG0280006368.01.T01 AT5G03340 43.210 81 45 1 2 81 206 286 5.49e-15 68.9
MsG0280006368.01.T01 AT5G03340 37.037 81 50 1 2 81 479 559 4.54e-11 57.8
MsG0280006368.01.T01 AT3G53230 43.210 81 45 1 2 81 207 287 5.49e-15 68.9
MsG0280006368.01.T01 AT3G53230 38.272 81 49 1 2 81 480 560 2.76e-11 58.5
MsG0280006368.01.T01 AT2G20140 37.179 78 48 1 2 78 187 264 4.47e-13 63.5
MsG0280006368.01.T01 AT4G29040 37.179 78 48 1 2 78 187 264 4.69e-13 63.5
MsG0280006368.01.T01 AT2G30950 41.772 79 46 0 2 80 226 304 1.49e-12 62.0
MsG0280006368.01.T01 AT2G30950 41.772 79 46 0 2 80 226 304 1.49e-12 62.0
MsG0280006368.01.T01 AT2G30950 41.772 79 46 0 2 80 226 304 1.49e-12 62.0
MsG0280006368.01.T01 AT2G30950 41.772 79 46 0 2 80 230 308 1.49e-12 62.0
MsG0280006368.01.T01 AT5G64580 43.038 79 45 0 2 80 315 393 1.69e-12 62.0
MsG0280006368.01.T01 AT2G29080 41.772 79 46 0 2 80 321 399 2.44e-12 61.6
MsG0280006368.01.T01 AT1G45000 36.667 90 51 2 2 85 138 227 2.65e-12 61.2
MsG0280006368.01.T01 AT5G43010 36.667 90 51 2 2 85 138 227 2.90e-12 61.2
MsG0280006368.01.T01 AT1G45000 36.667 90 51 2 2 85 138 227 2.90e-12 61.2
MsG0280006368.01.T01 AT1G06430 40.506 79 47 0 2 80 219 297 3.20e-12 61.2
MsG0280006368.01.T01 AT1G06430 40.506 79 47 0 2 80 219 297 3.20e-12 61.2
MsG0280006368.01.T01 AT1G06430 40.506 79 47 0 2 80 219 297 3.20e-12 61.2
MsG0280006368.01.T01 AT3G02450 40.449 89 46 3 2 84 333 420 5.55e-12 60.5
MsG0280006368.01.T01 AT1G53780 36.170 94 52 3 2 88 338 430 7.78e-12 60.1
MsG0280006368.01.T01 AT1G53780 36.170 94 52 3 2 88 339 431 7.78e-12 60.1
MsG0280006368.01.T01 AT1G53780 36.170 94 52 3 2 88 339 431 7.78e-12 60.1
MsG0280006368.01.T01 AT1G53780 36.170 94 52 3 2 88 360 452 7.80e-12 60.1
MsG0280006368.01.T01 AT3G47060 41.379 87 46 1 2 83 324 410 7.93e-12 60.1
MsG0280006368.01.T01 AT5G58870 40.230 87 47 1 2 83 328 414 9.55e-12 59.7
MsG0280006368.01.T01 AT5G15250 39.241 79 48 0 2 80 223 301 1.04e-11 59.7
MsG0280006368.01.T01 AT5G58290 39.744 78 46 1 2 78 154 231 1.08e-11 59.7
MsG0280006368.01.T01 AT1G53750 37.234 94 51 3 2 88 167 259 1.39e-11 59.3
MsG0280006368.01.T01 AT5G15250 39.241 79 48 0 2 80 223 301 1.56e-11 59.3
MsG0280006368.01.T01 AT4G28000 44.737 76 37 2 2 74 519 592 2.16e-11 58.9
MsG0280006368.01.T01 AT4G28000 44.737 76 37 2 2 74 517 590 2.17e-11 58.9
MsG0280006368.01.T01 AT4G28000 44.737 76 37 2 2 74 519 592 2.17e-11 58.9
MsG0280006368.01.T01 AT1G07510 40.506 79 47 0 2 80 326 404 2.66e-11 58.5
MsG0280006368.01.T01 AT1G64110 44.737 76 37 2 2 74 515 588 2.77e-11 58.5
MsG0280006368.01.T01 AT1G64110 44.737 76 37 2 2 74 518 591 2.77e-11 58.5
MsG0280006368.01.T01 AT1G64110 44.737 76 37 2 2 74 518 591 2.77e-11 58.5
MsG0280006368.01.T01 AT1G64110 44.737 76 37 2 2 74 520 593 2.77e-11 58.5
MsG0280006368.01.T01 AT1G64110 44.737 76 37 2 2 74 520 593 2.77e-11 58.5
MsG0280006368.01.T01 AT5G52882 44.737 76 37 2 2 74 516 589 3.50e-11 58.2
MsG0280006368.01.T01 AT1G50250 40.230 87 47 1 2 83 261 347 3.64e-11 58.2
MsG0280006368.01.T01 AT3G05530 38.356 73 44 1 2 73 170 242 4.35e-11 57.8
MsG0280006368.01.T01 AT5G42270 40.230 87 47 1 2 83 249 335 4.73e-11 57.8
MsG0280006368.01.T01 AT5G19990 40.000 75 44 1 5 78 163 237 5.22e-11 57.8
MsG0280006368.01.T01 AT5G19990 40.000 75 44 1 5 78 163 237 5.22e-11 57.8
MsG0280006368.01.T01 AT5G20000 40.000 75 44 1 5 78 163 237 5.22e-11 57.8
MsG0280006368.01.T01 AT1G09100 36.986 73 45 1 2 73 169 241 6.61e-11 57.4
MsG0280006368.01.T01 AT1G02890 43.902 82 40 3 2 79 943 1022 7.39e-11 57.4
MsG0280006368.01.T01 AT5G19990 40.000 75 44 1 5 78 105 179 7.39e-11 57.0

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGCTACCGAGGTGGTTTC+TGG 0.152839 2:+949897 MsG0280006368.01.T01:CDS
CCTGAGTTGCCTAAGCATTT+AGG 0.217130 2:+949768 MsG0280006368.01.T01:CDS
GGGGCCGATGTTTAAGGATT+TGG 0.289127 2:+949689 None:intergenic
GGGCCGATGTTTAAGGATTT+GGG 0.343509 2:+949690 None:intergenic
GGGTGGGATGAATGGTCTAT+TGG 0.361629 2:+949710 MsG0280006368.01.T01:CDS
AAGGATTTGGGTGGGATGAA+TGG 0.402781 2:+949702 MsG0280006368.01.T01:CDS
TTAGGAGTGAAACCGGTGAC+TGG 0.476508 2:+949786 MsG0280006368.01.T01:CDS
GAGGTGGTTTCTGGTGTCTC+AGG 0.480332 2:+949906 MsG0280006368.01.T01:CDS
TTGCATGGACCACCTGGTTG+TGG 0.485805 2:+949816 MsG0280006368.01.T01:CDS
TAAGCATTTAGGAGTGAAAC+CGG 0.510113 2:+949779 MsG0280006368.01.T01:CDS
CATGCTATTGCTAATGAGAC+TGG 0.515090 2:+949855 MsG0280006368.01.T01:CDS
CCTAAATGCTTAGGCAACTC+AGG 0.521039 2:-949768 None:intergenic
GTAGCCGAAATACGATGAAA+AGG 0.525021 2:-949882 None:intergenic
TAGGAGTGAAACCGGTGACT+GGG 0.551238 2:+949787 MsG0280006368.01.T01:CDS
TCTATTGGAGGAATTGATGA+TGG 0.566505 2:+949725 MsG0280006368.01.T01:CDS
TCGTATTTCGGCTACCGAGG+TGG 0.571424 2:+949890 MsG0280006368.01.T01:CDS
TGGGATGAATGGTCTATTGG+AGG 0.579132 2:+949713 MsG0280006368.01.T01:CDS
TGCAACAAAATCCCAGTCAC+CGG 0.580676 2:-949798 None:intergenic
TCATCGTATTTCGGCTACCG+AGG 0.583115 2:+949887 MsG0280006368.01.T01:CDS
CCGATGTTTAAGGATTTGGG+TGG 0.584057 2:+949693 None:intergenic
GGTTTCACTCCTAAATGCTT+AGG 0.585241 2:-949777 None:intergenic
CGATGTTTAAGGATTTGGGT+GGG 0.588724 2:+949694 None:intergenic
AGTCTAGTCTTTCCACAACC+AGG 0.590323 2:-949828 None:intergenic
CCACCCAAATCCTTAAACAT+CGG 0.591421 2:-949693 None:intergenic
CTAGTCTTTCCACAACCAGG+TGG 0.738162 2:-949825 None:intergenic
TGAGACACCAGAAACCACCT+CGG 0.753175 2:-949904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TATCCCAAGAGTAACAAAAA+TGG - Chr2:949947-949966 None:intergenic 30.0%
! TCTTCCTTTTCATCGTATTT+CGG + Chr2:949878-949897 MsG0280006368.01.T01:CDS 30.0%
!! CTTCCATTTTTGTTACTCTT+GGG + Chr2:949941-949960 MsG0280006368.01.T01:CDS 30.0%
!! TCTTCCATTTTTGTTACTCT+TGG + Chr2:949940-949959 MsG0280006368.01.T01:CDS 30.0%
TAAGCATTTAGGAGTGAAAC+CGG + Chr2:949779-949798 MsG0280006368.01.T01:CDS 35.0%
TCTATTGGAGGAATTGATGA+TGG + Chr2:949725-949744 MsG0280006368.01.T01:CDS 35.0%
CATGCTATTGCTAATGAGAC+TGG + Chr2:949855-949874 MsG0280006368.01.T01:CDS 40.0%
GGTTTCACTCCTAAATGCTT+AGG - Chr2:949780-949799 None:intergenic 40.0%
GTAGCCGAAATACGATGAAA+AGG - Chr2:949885-949904 None:intergenic 40.0%
AAGGATTTGGGTGGGATGAA+TGG + Chr2:949702-949721 MsG0280006368.01.T01:CDS 45.0%
CCTAAATGCTTAGGCAACTC+AGG - Chr2:949771-949790 None:intergenic 45.0%
CCTGAGTTGCCTAAGCATTT+AGG + Chr2:949768-949787 MsG0280006368.01.T01:CDS 45.0%
TGCAACAAAATCCCAGTCAC+CGG - Chr2:949801-949820 None:intergenic 45.0%
TGGGATGAATGGTCTATTGG+AGG + Chr2:949713-949732 MsG0280006368.01.T01:CDS 45.0%
! AGTCTAGTCTTTCCACAACC+AGG - Chr2:949831-949850 None:intergenic 45.0%
! ATTTTGTTGCATGGACCACC+TGG + Chr2:949810-949829 MsG0280006368.01.T01:CDS 45.0%
! GTGACTGGGATTTTGTTGCA+TGG + Chr2:949801-949820 MsG0280006368.01.T01:CDS 45.0%
GGGTGGGATGAATGGTCTAT+TGG + Chr2:949710-949729 MsG0280006368.01.T01:CDS 50.0%
TAGGAGTGAAACCGGTGACT+GGG + Chr2:949787-949806 MsG0280006368.01.T01:CDS 50.0%
TCATCGTATTTCGGCTACCG+AGG + Chr2:949887-949906 MsG0280006368.01.T01:CDS 50.0%
TGAGACACCAGAAACCACCT+CGG - Chr2:949907-949926 None:intergenic 50.0%
TTAGGAGTGAAACCGGTGAC+TGG + Chr2:949786-949805 MsG0280006368.01.T01:CDS 50.0%
! CTAGTCTTTCCACAACCAGG+TGG - Chr2:949828-949847 None:intergenic 50.0%
TCGTATTTCGGCTACCGAGG+TGG + Chr2:949890-949909 MsG0280006368.01.T01:CDS 55.0%
TTGCATGGACCACCTGGTTG+TGG + Chr2:949816-949835 MsG0280006368.01.T01:CDS 55.0%
!! GAGGTGGTTTCTGGTGTCTC+AGG + Chr2:949906-949925 MsG0280006368.01.T01:CDS 55.0%
TCGGCTACCGAGGTGGTTTC+TGG + Chr2:949897-949916 MsG0280006368.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 949696 949980 949696 ID=MsG0280006368.01;Name=MsG0280006368.01
Chr2 mRNA 949696 949980 949696 ID=MsG0280006368.01.T01;Parent=MsG0280006368.01;Name=MsG0280006368.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|94
Chr2 exon 949696 949980 949696 ID=MsG0280006368.01.T01:exon:955;Parent=MsG0280006368.01.T01
Chr2 CDS 949696 949980 949696 ID=MsG0280006368.01.T01:cds;Parent=MsG0280006368.01.T01
Gene Sequence

>MsG0280006368.01.T01

ATGTTTAAGGATTTGGGTGGGATGAATGGTCTATTGGAGGAATTGATGATGGATATTGTGTCATTGATTAATCCTGAGTTGCCTAAGCATTTAGGAGTGAAACCGGTGACTGGGATTTTGTTGCATGGACCACCTGGTTGTGGAAAGACTAGACTTGCTCATGCTATTGCTAATGAGACTGGTCTTCCTTTTCATCGTATTTCGGCTACCGAGGTGGTTTCTGGTGTCTCAGGTATTGCTGAAATCTTCCATTTTTGTTACTCTTGGGATAAGTTAATGTGCTAG

Protein sequence

>MsG0280006368.01.T01

MFKDLGGMNGLLEELMMDIVSLINPELPKHLGVKPVTGILLHGPPGCGKTRLAHAIANETGLPFHRISATEVVSGVSGIAEIFHFCYSWDKLMC*