AlfalfaGEDB | MsG0880042695.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042695.01

KEGG: K00679
GO: GO:0006629 GO:0008374
PFAM: PF02450

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042695.01.T01	XP_013444428.1	98.532	681	6	2	1	681	1	677	0	1389
MsG0880042695.01.T02	XP_013444428.1	98.532	681	6	2	1	681	1	677	0	1389

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042695.01.T01	Q9FNA9	73.684	684	164	5	1	681	1	671	0	1048
MsG0880042695.01.T02	Q9FNA9	73.684	684	164	5	1	681	1	671	0	1048

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042695.01.T01	A0A072TNQ7	98.532	681	6	2	1	681	1	677	0.0	1389
MsG0880042695.01.T02	A0A072TNQ7	98.532	681	6	2	1	681	1	677	0.0	1389

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048748.01	MsG0880042695.01	0.802678	5.005054e-49	2.466729e-46
MsG0080048912.01	MsG0880042695.01	0.831021	2.114071e-55	2.265879e-52
MsG0080048938.01	MsG0880042695.01	0.850953	1.216210e-60	2.423045e-57
MsG0180000114.01	MsG0880042695.01	0.808252	3.392448e-50	1.930371e-47
MsG0180000246.01	MsG0880042695.01	0.824940	6.147143e-54	5.524441e-51
MsG0180000784.01	MsG0880042695.01	0.823858	1.104231e-53	9.622030e-51
MsG0180000785.01	MsG0880042695.01	0.828142	1.059503e-54	1.044095e-51
MsG0180000843.01	MsG0880042695.01	0.835209	1.917182e-56	2.328493e-53
MsG0180001064.01	MsG0880042695.01	0.800896	1.162138e-48	5.474766e-46
MsG0180001100.01	MsG0880042695.01	0.837559	4.839665e-57	6.310102e-54

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042695.01.T01	MTR_8g019570	98.532	681	6	2	1	681	1	677	0.0	1389
MsG0880042695.01.T01	MTR_4g056140	52.484	644	278	11	55	681	6	638	0.0	670
MsG0880042695.01.T01	MTR_6g045280	54.286	105	31	2	230	334	38	125	4.28e-26	104
MsG0880042695.01.T01	MTR_4g125640	37.963	108	27	2	574	681	33	100	6.75e-16	74.3
MsG0880042695.01.T02	MTR_8g019570	98.532	681	6	2	1	681	1	677	0.0	1389
MsG0880042695.01.T02	MTR_4g056140	52.484	644	278	11	55	681	6	638	0.0	670
MsG0880042695.01.T02	MTR_6g045280	54.286	105	31	2	230	334	38	125	4.28e-26	104
MsG0880042695.01.T02	MTR_4g125640	37.963	108	27	2	574	681	33	100	6.75e-16	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042695.01.T01	AT5G13640	73.684	684	164	5	1	681	1	671	0.0	1048
MsG0880042695.01.T01	AT5G13640	77.203	522	116	2	163	681	6	527	0.0	837
MsG0880042695.01.T01	AT5G13640	77.087	515	115	2	170	681	1	515	0.0	823
MsG0880042695.01.T01	AT3G44830	56.231	674	261	10	26	681	8	665	0.0	785
MsG0880042695.01.T01	AT1G04010	25.792	221	150	5	124	334	44	260	6.11e-15	79.0
MsG0880042695.01.T02	AT5G13640	73.684	684	164	5	1	681	1	671	0.0	1048
MsG0880042695.01.T02	AT5G13640	77.203	522	116	2	163	681	6	527	0.0	837
MsG0880042695.01.T02	AT5G13640	77.087	515	115	2	170	681	1	515	0.0	823
MsG0880042695.01.T02	AT3G44830	56.231	674	261	10	26	681	8	665	0.0	785
MsG0880042695.01.T02	AT1G04010	25.792	221	150	5	124	334	44	260	6.11e-15	79.0

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Find 127 sgRNAs with CRISPR-Local

Find 419 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAGATTCAATCCTTC+AGG	0.299444	8:-12700711	MsG0880042695.01.T01:CDS
AAAACATTAGATTTGTTGTA+TGG	0.353623	8:-12700149	MsG0880042695.01.T01:three_prime_UTR
AAAAGGGTCAGAACCAGAAA+AGG	0.444668	8:-12705799	MsG0880042695.01.T01:CDS
AAACAAGATTCAATCCTTCA+GGG	0.585693	8:-12700710	MsG0880042695.01.T01:CDS
AAACCAGGTGGATCAGGCAT+AGG	0.590698	8:+12705513	None:intergenic
AAAGGGTCAGAACCAGAAAA+GGG	0.549984	8:-12705798	MsG0880042695.01.T01:CDS
AAAGTTAGTTGTCTGAAAGA+TGG	0.594243	8:-12700810	MsG0880042695.01.T01:CDS
AAGTTACCTTTGTGAGGGGT+TGG	0.447573	8:-12700483	MsG0880042695.01.T01:exon
AATATTAGCATCGCACGGAG+AGG	0.664426	8:+12706139	None:intergenic
AATCAAGTTACCTTTGTGAG+GGG	0.617198	8:-12700487	MsG0880042695.01.T01:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGCAACATAGAACTCA+TGG	-	Chr8:12702655-12702674	MsG0880042695.01.T01:CDS	30.0%
AAAACAAGATTCAATCCTTC+AGG	-	Chr8:12705474-12705493	MsG0880042695.01.T01:CDS	30.0%
AAACAAGATTCAATCCTTCA+GGG	-	Chr8:12705475-12705494	MsG0880042695.01.T01:CDS	30.0%
AAAGTTAGTTGTCTGAAAGA+TGG	-	Chr8:12705375-12705394	MsG0880042695.01.T01:CDS	30.0%
AACAATGTCAATGATACCAA+AGG	-	Chr8:12703407-12703426	MsG0880042695.01.T01:CDS	30.0%
AATATCATTTCAGAACACTG+AGG	-	Chr8:12702512-12702531	MsG0880042695.01.T01:intron	30.0%
AATTAAGCTGTTTATCCAAC+AGG	-	Chr8:12703877-12703896	MsG0880042695.01.T01:CDS	30.0%
ACAATGTCAATGATACCAAA+GGG	-	Chr8:12703408-12703427	MsG0880042695.01.T01:CDS	30.0%
ACACAAATTCTCATTGCATT+TGG	-	Chr8:12703216-12703235	MsG0880042695.01.T01:intron	30.0%
ACTAAAATATTAGCATCGCA+CGG	+	Chr8:12700054-12700073	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	12699967	12706240	12699967	ID=MsG0880042695.01;Name=MsG0880042695.01
Chr8	mRNA	12699967	12706227	12699967	ID=MsG0880042695.01.T01;Parent=MsG0880042695.01;Name=MsG0880042695.01.T01;_AED=0.29;_eAED=0.29;_QI=198\|1\|1\|1\|0.83\|0.71\|7\|524\|681
Chr8	exon	12699967	12700974	12699967	ID=MsG0880042695.01.T01:exon:3664;Parent=MsG0880042695.01.T01
Chr8	exon	12705357	12705982	12705357	ID=MsG0880042695.01.T01:exon:3659;Parent=MsG0880042695.01.T01
Chr8	exon	12706171	12706227	12706171	ID=MsG0880042695.01.T01:exon:3658;Parent=MsG0880042695.01.T01
Chr8	five_prime_UTR	12706171	12706227	12706171	ID=MsG0880042695.01.T01:five_prime_utr;Parent=MsG0880042695.01.T01
Chr8	five_prime_UTR	12705842	12705982	12705842	ID=MsG0880042695.01.T01:five_prime_utr;Parent=MsG0880042695.01.T01
Chr8	CDS	12705357	12705841	12705357	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	CDS	12703674	12703896	12703674	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	CDS	12703295	12703578	12703295	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	CDS	12702578	12702887	12702578	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	CDS	12701958	12702217	12701958	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	CDS	12700491	12700974	12700491	ID=MsG0880042695.01.T01:cds;Parent=MsG0880042695.01.T01
Chr8	three_prime_UTR	12699967	12700490	12699967	ID=MsG0880042695.01.T01:three_prime_utr;Parent=MsG0880042695.01.T01
Chr8	mRNA	12699970	12706240	12699970	ID=MsG0880042695.01.T02;Parent=MsG0880042695.01;Name=MsG0880042695.01.T02;_AED=0.27;_eAED=0.27;_QI=399\|1\|1\|1\|1\|1\|6\|521\|681
Chr8	exon	12699970	12700974	12699970	ID=MsG0880042695.01.T02:exon:3666;Parent=MsG0880042695.01.T02
Chr8	exon	12705357	12706240	12705357	ID=MsG0880042695.01.T02:exon:3665;Parent=MsG0880042695.01.T02
Chr8	five_prime_UTR	12705842	12706240	12705842	ID=MsG0880042695.01.T02:five_prime_utr;Parent=MsG0880042695.01.T02
Chr8	CDS	12705357	12705841	12705357	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	CDS	12703674	12703896	12703674	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	CDS	12703295	12703578	12703295	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	CDS	12702578	12702887	12702578	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	CDS	12701958	12702217	12701958	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	CDS	12700491	12700974	12700491	ID=MsG0880042695.01.T02:cds;Parent=MsG0880042695.01.T02
Chr8	three_prime_UTR	12699970	12700490	12699970	ID=MsG0880042695.01.T02:three_prime_utr;Parent=MsG0880042695.01.T02

Gene Sequence

>MsG0880042695.01.T02

ATGGCTTTGATCCGAAGAAGAAAAGGGTCAGAACCAGAAAAGGGTAGTAGCGAAAATTCAGAACCAAAAATTCAGAAAAAAGAAGAAGAAGAAGAAGAAGATGATGATAATGATAAGAACAAAAAGAACAAGAAGAAAAACAAGGACGAGGTTGCTGTTATTAAGAAGAAGAGTACTAGGTGGTCTTGTGTTGACAGTTGTTGTTGGTTTGTTGGTTGCATTTGTACTTTATGGTGGTTTTTGTTGTTTATGTATAATGTAATGCCAGCTTCATTTCCTCAGTATGTCACTGAGGCTATAACTGGTCCTATGCCTGATCCACCTGGTTTGAAATTGAAGAAAGAAGGATTGAGTGTGAAGCATCCAGTTGTTTTTGTTCCGGGGATTGTTACCGGTGGACTTGAACTTTGGGAGGGGCATCAGTGTGCTGAAGGATTGTTTAGGAAGAGGTTGTGGGGTGGTACTTTTGGAGAAGTTTATAAAAGACCTTCATGCTGGGTGGAGCACATGTCCCTTGACAATGAAACAGGAATGGATCCACCTGGCATTAGAGTCCGGCCTGTCTCAGGACTTGTAGCTGCTGACTACTTTGCCGCAGGATACTTTGTTTGGGCGGTCCTAATTGCTAACTTGGCACGCATCGGTTATGAAGAAAAAACTATGTACATGGCTGCATATGATTGGAGAATATCATTTCAGAACACTGAGGTGCGGGATCAAACACTAAGTCGGATAAAAAGCAACATAGAACTCATGGTTTCCACTAATGGTGGAAATAAGGCGGTTATTATTCCACATTCGATGGGGGTTTTGTATTTTCTTCATTTCATGAAATGGGTTGAAGCACCAGCTCCAATGGGTGGTGGAGGAGGACCAGATTGGTGTTCCAAATATATTAAGGCAATTGTAAACATCGGTGGACCATTTTTAGGTGTTCCAAAGGCTATAGCAGGGCTTTTCTCAGCTGAAGCCCGAGACATTGCTGTTGCAAGGGCGATTGCACCAGGGTTTTTAGATAACGATATGTTCCGCATTCAAACATTGCAACATGTTATGAAGATGACTCGCACTTGGGACTCAACAATGTCAATGATACCAAAGGGAGGGGACACCATATGGGGCGGCCTTGATTGGTCACCAGAGGAAAGCTGTGGCCTTCACACGAGAAAGCAAAGCAGCAACAATACTCAGTTGACAGAACAAAAGACAAATAAAACAAATGTTAACTATGGAAGAATGATATCGTTTGGCAAAGCTGTCGCAGAGGCAGATTCGTCTAAGATCGATATTGTTGACTTTCGGGGTGCAATTAAGGGTCCCAATGTTGCAAATACCTCTTGTCGTGATGTGTGGACTGAATACCATGATATGGGAGTCGAGGGAGTAAGAGCAGTTGCAGAACATAAAGTTTACACAGCTGGCTCAATCATAGACCTGCTTCAGTTTGTTGCTCCGAAAATGATGGCGCGTGGTAGTGATCATTTCTCTTATGGAGTAGCTGACAATTTAGATGATCCTAAATATGAACACTACAAATATTGGTCAAACCCATTGGAGACAAAGTTACCAAATGCCCCTGATATGGAAATCTTCTCTCTATATGGAGTTGGCCTACCAACCGAAAGAGCTTATATTTATAAGTTAACTCCCTTTGCCGAGTGTTACATTCCTTTTGAAATTGATCCTAATGCCGATGGTGGTGACAAAGTTAGTTGTCTGAAAGATGGTGTCTACACTGTTGATGGTGATGAGACCGTTCCAGTTCTAAGTTCAGGCTACATGTGTGCTAAAGGTTGGCGCGGAAAAACAAGATTCAATCCTTCAGGGATCCGCACTTATGTTCGAGAATATGATCATTCTCCTCCAGCCAACCTGCTAGAAGGTAGAGGAACACAAAGCGGTGCTCATGTTGATATAATGGGAAACTTTGCGTTGATTGAAGATGTTATGAGGGTTGCAGCAGGAGCCAAAGGAGAAGAATTGGGAGGTGATAAGGTGTATTCTGATATCTTTAAATGGTCCGATAGAATCAAGTTACCTTTGTGA

>MsG0880042695.01.T01

Protein sequence

>MsG0880042695.01.T02

MALIRRRKGSEPEKGSSENSEPKIQKKEEEEEEDDDNDKNKKNKKKNKDEVAVIKKKSTRWSCVDSCCWFVGCICTLWWFLLFMYNVMPASFPQYVTEAITGPMPDPPGLKLKKEGLSVKHPVVFVPGIVTGGLELWEGHQCAEGLFRKRLWGGTFGEVYKRPSCWVEHMSLDNETGMDPPGIRVRPVSGLVAADYFAAGYFVWAVLIANLARIGYEEKTMYMAAYDWRISFQNTEVRDQTLSRIKSNIELMVSTNGGNKAVIIPHSMGVLYFLHFMKWVEAPAPMGGGGGPDWCSKYIKAIVNIGGPFLGVPKAIAGLFSAEARDIAVARAIAPGFLDNDMFRIQTLQHVMKMTRTWDSTMSMIPKGGDTIWGGLDWSPEESCGLHTRKQSSNNTQLTEQKTNKTNVNYGRMISFGKAVAEADSSKIDIVDFRGAIKGPNVANTSCRDVWTEYHDMGVEGVRAVAEHKVYTAGSIIDLLQFVAPKMMARGSDHFSYGVADNLDDPKYEHYKYWSNPLETKLPNAPDMEIFSLYGVGLPTERAYIYKLTPFAECYIPFEIDPNADGGDKVSCLKDGVYTVDGDETVPVLSSGYMCAKGWRGKTRFNPSGIRTYVREYDHSPPANLLEGRGTQSGAHVDIMGNFALIEDVMRVAAGAKGEELGGDKVYSDIFKWSDRIKLPL*

>MsG0880042695.01.T01