AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036443.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036443.01.T01 MTR_7g018880 94.086 186 11 0 1 186 1 186 1.02e-129 363
MsG0780036443.01.T01 MTR_5g010050 32.039 103 70 0 77 179 82 184 1.65e-20 86.3
MsG0780036443.01.T01 MTR_3g090440 32.727 110 74 0 77 186 85 194 3.28e-20 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036443.01.T01 AT1G71480 73.394 109 29 0 77 185 70 178 7.28e-57 177
MsG0780036443.01.T01 AT1G71480 73.394 109 29 0 77 185 70 178 4.85e-56 176
MsG0780036443.01.T01 AT1G71480 67.073 82 27 0 77 158 70 151 8.85e-36 122
MsG0780036443.01.T01 AT5G41470 39.796 98 59 0 77 174 74 171 8.02e-24 95.1

Find 34 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGCCTTAGTAACTGAATT+TGG 0.223319 7:-8203713 None:intergenic
ATTGGAGTGATTTGGCATTT+AGG 0.250362 7:+8204202 MsG0780036443.01.T01:CDS
CATCATCAATTGGAGTGATT+TGG 0.259310 7:+8204194 MsG0780036443.01.T01:CDS
AGTGGAGCATAAAATGTTTA+TGG 0.309641 7:+8203604 MsG0780036443.01.T01:exon
GTCTTTCCTCGTCCATTTGT+GGG 0.321615 7:+8203947 MsG0780036443.01.T01:CDS
AAGAGGTAGAAAATGGAATA+TGG 0.354460 7:+8203748 MsG0780036443.01.T01:CDS
AACAAACTGCAGATCCTTGC+TGG 0.380904 7:-8204146 None:intergenic
TGTCTTTCCTCGTCCATTTG+TGG 0.381094 7:+8203946 MsG0780036443.01.T01:CDS
AAACTGCATCCTTTGCTAAA+GGG 0.439731 7:-8205068 None:intergenic
TTGAAGAGTCTATGTGGTAC+TGG 0.444533 7:+8203577 MsG0780036443.01.T01:five_prime_UTR
TGTTCCAAATTCAGTTACTA+AGG 0.449806 7:+8203709 MsG0780036443.01.T01:CDS
GTTGTGAGAAATTTCTATGA+GGG 0.454604 7:+8203857 MsG0780036443.01.T01:CDS
CATCCTTTGCTAAAGGGAAA+AGG 0.457962 7:-8205062 None:intergenic
AAAACTGCATCCTTTGCTAA+AGG 0.460379 7:-8205069 None:intergenic
ACGGAGGATTCATCATCAAT+TGG 0.463451 7:+8204184 MsG0780036443.01.T01:CDS
AATACCTTACGACCCACAAA+TGG 0.470344 7:-8203959 None:intergenic
CTCTGAAAATTGTGTGTATG+AGG 0.478927 7:+8203919 MsG0780036443.01.T01:CDS
AATTCAGTTACTAAGGCTGT+TGG 0.493447 7:+8203716 MsG0780036443.01.T01:CDS
TCGTCCATTTGTGGGTCGTA+AGG 0.515707 7:+8203955 MsG0780036443.01.T01:CDS
AGTTGTGAGAAATTTCTATG+AGG 0.518932 7:+8203856 MsG0780036443.01.T01:CDS
TGTTATTGATGATTTGTCTA+CGG 0.524833 7:+8204165 MsG0780036443.01.T01:CDS
CTATGTGGTACTGGGTGAAG+TGG 0.575860 7:+8203586 MsG0780036443.01.T01:five_prime_UTR
TATCGCTTGGAGGTGATCAA+CGG 0.583040 7:+8205092 MsG0780036443.01.T01:CDS
CTATGTGACAAAGAATTCAA+AGG 0.597778 7:-8203677 None:intergenic
TTAAGTTTGAAGAGTCTATG+TGG 0.602776 7:+8203571 MsG0780036443.01.T01:five_prime_UTR
GGGAATCAATGCACATGATG+TGG 0.616754 7:+8203877 MsG0780036443.01.T01:CDS
GTTCACTGATTCTACCAGCA+AGG 0.619438 7:+8204132 MsG0780036443.01.T01:CDS
TATTGATGATTTGTCTACGG+AGG 0.632550 7:+8204168 MsG0780036443.01.T01:CDS
AACACACTTTGATGATAACA+AGG 0.634727 7:+8203814 MsG0780036443.01.T01:CDS
TTACTGAGTGCAGAATGGAA+AGG 0.655983 7:+8205035 MsG0780036443.01.T01:intron
TGAAGAGTCTATGTGGTACT+GGG 0.670990 7:+8203578 MsG0780036443.01.T01:five_prime_UTR
TTACGACCCACAAATGGACG+AGG 0.682465 7:-8203953 None:intergenic
AATATGGCAGTTAATAACAG+TGG 0.699800 7:+8203764 MsG0780036443.01.T01:CDS
CAAATGGACGAGGAAAGACA+AGG 0.709032 7:-8203943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATAAGTAAATTTCAAA+AGG + Chr7:8204991-8205010 MsG0780036443.01.T01:intron 10.0%
!!! AAAAGAAATATATTTAACTT+TGG + Chr7:8204032-8204051 MsG0780036443.01.T01:intron 10.0%
!!! AATATATTTCTTTTTTTTAG+GGG - Chr7:8204026-8204045 None:intergenic 10.0%
!!! TTTGATTAAATATTTAACTT+TGG + Chr7:8203999-8204018 MsG0780036443.01.T01:intron 10.0%
!! AAAAACTTACTACTAAATTA+GGG - Chr7:8204535-8204554 None:intergenic 15.0%
!! AAAACTTACTACTAAATTAG+GGG - Chr7:8204534-8204553 None:intergenic 20.0%
!! AAATAAGATAAAATGAGACA+TGG - Chr7:8204975-8204994 None:intergenic 20.0%
!! AATATAAATTGAGTGATACT+TGG + Chr7:8204502-8204521 MsG0780036443.01.T01:intron 20.0%
!! ATATAAATTGAGTGATACTT+GGG + Chr7:8204503-8204522 MsG0780036443.01.T01:intron 20.0%
!! CAAAAACTTACTACTAAATT+AGG - Chr7:8204536-8204555 None:intergenic 20.0%
!! TAGAAGAAGTTTATACAAAT+TGG + Chr7:8204069-8204088 MsG0780036443.01.T01:intron 20.0%
!!! AGAAATATATTTAACTTTGG+TGG + Chr7:8204035-8204054 MsG0780036443.01.T01:intron 20.0%
!!! ATTGGTTTCTTAATTATTAC+AGG + Chr7:8204087-8204106 MsG0780036443.01.T01:intron 20.0%
!!! CTAATTTAGTAGTAAGTTTT+TGG + Chr7:8204534-8204553 MsG0780036443.01.T01:intron 20.0%
!!! TTTCAATCATTTTCAAAATG+CGG - Chr7:8204904-8204923 None:intergenic 20.0%
! ATAAATAGGATCACAACAAT+CGG - Chr7:8204592-8204611 None:intergenic 25.0%
! TATAAATTGAGTGATACTTG+GGG + Chr7:8204504-8204523 MsG0780036443.01.T01:intron 25.0%
! TTAGAAAAAGAGGTAGAAAA+TGG + Chr7:8203741-8203760 MsG0780036443.01.T01:CDS 25.0%
!! TGTTATTGATGATTTGTCTA+CGG + Chr7:8204165-8204184 MsG0780036443.01.T01:CDS 25.0%
AAGAGGTAGAAAATGGAATA+TGG + Chr7:8203748-8203767 MsG0780036443.01.T01:CDS 30.0%
AATATGGCAGTTAATAACAG+TGG + Chr7:8203764-8203783 MsG0780036443.01.T01:CDS 30.0%
ACAATATGACGAGTATTCAT+TGG - Chr7:8204862-8204881 None:intergenic 30.0%
AGTGGAGCATAAAATGTTTA+TGG + Chr7:8203604-8203623 MsG0780036443.01.T01:exon 30.0%
AGTTGTGAGAAATTTCTATG+AGG + Chr7:8203856-8203875 MsG0780036443.01.T01:CDS 30.0%
CTATGTGACAAAGAATTCAA+AGG - Chr7:8203680-8203699 None:intergenic 30.0%
CTCTGATGTCAAAGTAATTA+AGG + Chr7:8204447-8204466 MsG0780036443.01.T01:intron 30.0%
GATGTCAAAGTAATTAAGGA+AGG + Chr7:8204451-8204470 MsG0780036443.01.T01:intron 30.0%
GTTGTGAGAAATTTCTATGA+GGG + Chr7:8203857-8203876 MsG0780036443.01.T01:CDS 30.0%
TGTTCCAAATTCAGTTACTA+AGG + Chr7:8203709-8203728 MsG0780036443.01.T01:CDS 30.0%
TTAAGTTTGAAGAGTCTATG+TGG + Chr7:8203571-8203590 MsG0780036443.01.T01:five_prime_UTR 30.0%
! AACACACTTTGATGATAACA+AGG + Chr7:8203814-8203833 MsG0780036443.01.T01:CDS 30.0%
AAAACTGCATCCTTTGCTAA+AGG - Chr7:8205072-8205091 None:intergenic 35.0%
AAACTGCATCCTTTGCTAAA+GGG - Chr7:8205071-8205090 None:intergenic 35.0%
AAGAACTGTTGTTGAGATAC+TGG - Chr7:8204733-8204752 None:intergenic 35.0%
AATTCAGTTACTAAGGCTGT+TGG + Chr7:8203716-8203735 MsG0780036443.01.T01:CDS 35.0%
ACAGCCTTAGTAACTGAATT+TGG - Chr7:8203716-8203735 None:intergenic 35.0%
CATCATCAATTGGAGTGATT+TGG + Chr7:8204194-8204213 MsG0780036443.01.T01:CDS 35.0%
! ATTGGAGTGATTTGGCATTT+AGG + Chr7:8204202-8204221 MsG0780036443.01.T01:CDS 35.0%
! CTCTGAAAATTGTGTGTATG+AGG + Chr7:8203919-8203938 MsG0780036443.01.T01:CDS 35.0%
! TATTGATGATTTGTCTACGG+AGG + Chr7:8204168-8204187 MsG0780036443.01.T01:CDS 35.0%
! TTTTGTTACTGAGTGCAGAA+TGG + Chr7:8205030-8205049 MsG0780036443.01.T01:intron 35.0%
!! ATCTGATTGGGCCATTTTAT+TGG + Chr7:8204798-8204817 MsG0780036443.01.T01:intron 35.0%
!! TCTGATTGGGCCATTTTATT+GGG + Chr7:8204799-8204818 MsG0780036443.01.T01:intron 35.0%
AACTGTTGTTGAGATACTGG+TGG - Chr7:8204730-8204749 None:intergenic 40.0%
AAGTACTTTCTTCGCTGCTT+TGG - Chr7:8204418-8204437 None:intergenic 40.0%
AATACCTTACGACCCACAAA+TGG - Chr7:8203962-8203981 None:intergenic 40.0%
ACAACAATCGGTCAGATGAT+TGG - Chr7:8204580-8204599 None:intergenic 40.0%
ACGGAGGATTCATCATCAAT+TGG + Chr7:8204184-8204203 MsG0780036443.01.T01:CDS 40.0%
AGCCCAATATGTATAGATCG+AGG - Chr7:8204830-8204849 None:intergenic 40.0%
ATCCAAGTGCACAACTACAA+TGG - Chr7:8204680-8204699 None:intergenic 40.0%
ATTAAGGAAGGATGAAGAGC+AGG + Chr7:8204463-8204482 MsG0780036443.01.T01:intron 40.0%
CATCCTTTGCTAAAGGGAAA+AGG - Chr7:8205065-8205084 None:intergenic 40.0%
GCTGTTGGAGTTAGAAAAAG+AGG + Chr7:8203731-8203750 MsG0780036443.01.T01:CDS 40.0%
GGCCTCGATCTATACATATT+GGG + Chr7:8204825-8204844 MsG0780036443.01.T01:intron 40.0%
TGAAGAGTCTATGTGGTACT+GGG + Chr7:8203578-8203597 MsG0780036443.01.T01:five_prime_UTR 40.0%
TGGCCTCGATCTATACATAT+TGG + Chr7:8204824-8204843 MsG0780036443.01.T01:intron 40.0%
TGTTGAGGTAGTAATCAGTG+CGG + Chr7:8204626-8204645 MsG0780036443.01.T01:intron 40.0%
TTAAGGAAGGATGAAGAGCA+GGG + Chr7:8204464-8204483 MsG0780036443.01.T01:intron 40.0%
TTACTGAGTGCAGAATGGAA+AGG + Chr7:8205035-8205054 MsG0780036443.01.T01:intron 40.0%
TTGAAGAGTCTATGTGGTAC+TGG + Chr7:8203577-8203596 MsG0780036443.01.T01:five_prime_UTR 40.0%
! AAGCCTTTTCCCTTTAGCAA+AGG + Chr7:8205059-8205078 MsG0780036443.01.T01:CDS 40.0%
! AGGATGCAGTTTTTATCGCT+TGG + Chr7:8205079-8205098 MsG0780036443.01.T01:CDS 40.0%
! ATGCAGTTTTTATCGCTTGG+AGG + Chr7:8205082-8205101 MsG0780036443.01.T01:CDS 40.0%
!! ACAACTACAATGGTGGATAC+CGG - Chr7:8204670-8204689 None:intergenic 40.0%
AACAAACTGCAGATCCTTGC+TGG - Chr7:8204149-8204168 None:intergenic 45.0%
CAAATGGACGAGGAAAGACA+AGG - Chr7:8203946-8203965 None:intergenic 45.0%
CAAGTGCACAACTACAATGG+TGG - Chr7:8204677-8204696 None:intergenic 45.0%
CACCATTGTAGTTGTGCACT+TGG + Chr7:8204675-8204694 MsG0780036443.01.T01:intron 45.0%
GAAGTTACGATTCTGTTCCC+CGG + Chr7:8204648-8204667 MsG0780036443.01.T01:intron 45.0%
GATCCTATTTATCCCGCTCT+TGG + Chr7:8204600-8204619 MsG0780036443.01.T01:intron 45.0%
GATTGGAGCGTATCACAACT+CGG - Chr7:8204563-8204582 None:intergenic 45.0%
GGGAATCAATGCACATGATG+TGG + Chr7:8203877-8203896 MsG0780036443.01.T01:CDS 45.0%
GTCTTTCCTCGTCCATTTGT+GGG + Chr7:8203947-8203966 MsG0780036443.01.T01:CDS 45.0%
GTTCCAAGAGCGGGATAAAT+AGG - Chr7:8204606-8204625 None:intergenic 45.0%
TACCTCAACAGTTCCAAGAG+CGG - Chr7:8204616-8204635 None:intergenic 45.0%
TAGCTCGAGCTCATCTGATT+GGG + Chr7:8204786-8204805 MsG0780036443.01.T01:intron 45.0%
TATCGCTTGGAGGTGATCAA+CGG + Chr7:8205092-8205111 MsG0780036443.01.T01:CDS 45.0%
TGTCTTTCCTCGTCCATTTG+TGG + Chr7:8203946-8203965 MsG0780036443.01.T01:CDS 45.0%
TTAGCTCGAGCTCATCTGAT+TGG + Chr7:8204785-8204804 MsG0780036443.01.T01:intron 45.0%
! TACTTTCTTCGCTGCTTTGG+AGG - Chr7:8204415-8204434 None:intergenic 45.0%
!! AACTACAATGGTGGATACCG+GGG - Chr7:8204668-8204687 None:intergenic 45.0%
!! CAACTACAATGGTGGATACC+GGG - Chr7:8204669-8204688 None:intergenic 45.0%
!! GTTCACTGATTCTACCAGCA+AGG + Chr7:8204132-8204151 MsG0780036443.01.T01:CDS 45.0%
!! TTGGGCCATTTTATTGGGTC+TGG + Chr7:8204804-8204823 MsG0780036443.01.T01:intron 45.0%
!! ATTTAATCAAAATAAATTTA+TGG - Chr7:8203990-8204009 None:intergenic 5.0%
!!! AAATATATTTCTTTTTTTTA+GGG - Chr7:8204027-8204046 None:intergenic 5.0%
!!! TAAATATATTTCTTTTTTTT+AGG - Chr7:8204028-8204047 None:intergenic 5.0%
ACCTCAACAGTTCCAAGAGC+GGG - Chr7:8204615-8204634 None:intergenic 50.0%
CGAGGCCAGACCCAATAAAA+TGG - Chr7:8204812-8204831 None:intergenic 50.0%
GAGCAGGAGGTTCATATCAC+CGG - Chr7:8204380-8204399 None:intergenic 50.0%
GAGCTAAGAAAGCTAGGCGT+GGG - Chr7:8204772-8204791 None:intergenic 50.0%
GAGCTCGAGCTAAGAAAGCT+AGG - Chr7:8204778-8204797 None:intergenic 50.0%
TCGTCCATTTGTGGGTCGTA+AGG + Chr7:8203955-8203974 MsG0780036443.01.T01:CDS 50.0%
TTACGACCCACAAATGGACG+AGG - Chr7:8203956-8203975 None:intergenic 50.0%
! CTATGTGGTACTGGGTGAAG+TGG + Chr7:8203586-8203605 MsG0780036443.01.T01:five_prime_UTR 50.0%
CACACGTTGATCGCATGAGC+CGG + Chr7:8204358-8204377 MsG0780036443.01.T01:intron 55.0%
CGAGCTAAGAAAGCTAGGCG+TGG - Chr7:8204773-8204792 None:intergenic 55.0%
CTAGGCGTGGGATGAGAACT+CGG - Chr7:8204760-8204779 None:intergenic 55.0%
GAGCGTACTAGAAGAGCAGG+AGG - Chr7:8204393-8204412 None:intergenic 55.0%
TCCCGCTCTTGGAACTGTTG+AGG + Chr7:8204611-8204630 MsG0780036443.01.T01:intron 55.0%
TGGAGCGTATCACAACTCGG+AGG - Chr7:8204560-8204579 None:intergenic 55.0%
! GAGGAGCGTACTAGAAGAGC+AGG - Chr7:8204396-8204415 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 8203565 8205133 8203565 ID=MsG0780036443.01;Name=MsG0780036443.01
Chr7 mRNA 8203565 8205133 8203565 ID=MsG0780036443.01.T01;Parent=MsG0780036443.01;Name=MsG0780036443.01.T01;_AED=0.50;_eAED=0.50;_QI=52|1|0.66|1|1|1|3|0|186
Chr7 exon 8203565 8203976 8203565 ID=MsG0780036443.01.T01:exon:63575;Parent=MsG0780036443.01.T01
Chr7 exon 8204109 8204223 8204109 ID=MsG0780036443.01.T01:exon:63576;Parent=MsG0780036443.01.T01
Chr7 exon 8205048 8205133 8205048 ID=MsG0780036443.01.T01:exon:63577;Parent=MsG0780036443.01.T01
Chr7 five_prime_UTR 8203565 8203616 8203565 ID=MsG0780036443.01.T01:five_prime_utr;Parent=MsG0780036443.01.T01
Chr7 CDS 8203617 8203976 8203617 ID=MsG0780036443.01.T01:cds;Parent=MsG0780036443.01.T01
Chr7 CDS 8204109 8204223 8204109 ID=MsG0780036443.01.T01:cds;Parent=MsG0780036443.01.T01
Chr7 CDS 8205048 8205133 8205048 ID=MsG0780036443.01.T01:cds;Parent=MsG0780036443.01.T01
Gene Sequence

>MsG0780036443.01.T01

ATGTTTATGGTGTGTAGTTCTAGTAGCAGCACACAAGTGCTAATATTGAACTCAGCTTCACCTTTGAATTCTTTGTCACATAGAAGAATCTATGTTCCAAATTCAGTTACTAAGGCTGTTGGAGTTAGAAAAAGAGGTAGAAAATGGAATATGGCAGTTAATAACAGTGGTGAAAATAATTCAATTACAACAACTACAACACACTTTGATGATAACAAGGTTGTAGATTCAGCTTCAAAAGTTGTGAGAAATTTCTATGAGGGAATCAATGCACATGATGTGGATTCTGTACAATATCTTATCTCTGAAAATTGTGTGTATGAGGACCTTGTCTTTCCTCGTCCATTTGTGGGTCGTAAGGAAATTATAGAGTTCTTCAAAAAGTTCACTGATTCTACCAGCAAGGATCTGCAGTTTGTTATTGATGATTTGTCTACGGAGGATTCATCATCAATTGGAGTGATTTGGCATTTAGAATGGAAAGGAAAGCCTTTTCCCTTTAGCAAAGGATGCAGTTTTTATCGCTTGGAGGTGATCAACGGCAAGAGACAAATCACGTAA

Protein sequence

>MsG0780036443.01.T01

MFMVCSSSSSTQVLILNSASPLNSLSHRRIYVPNSVTKAVGVRKRGRKWNMAVNNSGENNSITTTTTHFDDNKVVDSASKVVRNFYEGINAHDVDSVQYLISENCVYEDLVFPRPFVGRKEIIEFFKKFTDSTSKDLQFVIDDLSTEDSSSIGVIWHLEWKGKPFPFSKGCSFYRLEVINGKRQIT*