AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024788.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024788.01.T01 MTR_5g016540 96.104 77 3 0 1 77 1 77 4.97e-48 150
MsG0580024788.01.T01 MTR_6g005820 83.117 77 13 0 1 77 1 77 4.24e-41 132
MsG0580024788.01.T01 MTR_5g034130 83.117 77 13 0 1 77 1 77 4.38e-41 132
MsG0580024788.01.T01 MTR_5g034125 83.117 77 13 0 1 77 1 77 4.38e-41 132
MsG0580024788.01.T01 MTR_7g109960 81.818 77 14 0 1 77 1 77 1.01e-40 132
MsG0580024788.01.T01 MTR_1g086480 81.818 77 14 0 1 77 1 77 1.13e-40 132
MsG0580024788.01.T01 MTR_5g007810 62.338 77 29 0 1 77 1 77 9.19e-31 107
MsG0580024788.01.T01 MTR_4g077640 62.338 77 29 0 1 77 1 77 1.96e-30 106
MsG0580024788.01.T01 MTR_4g128910 57.500 80 33 1 1 79 1 80 1.21e-29 103
MsG0580024788.01.T01 MTR_5g007820 60.274 73 29 0 1 73 1 73 2.31e-27 98.2
MsG0580024788.01.T01 MTR_1g076550 56.757 74 32 0 1 74 1 74 6.05e-25 92.0
MsG0580024788.01.T01 MTR_5g007840 58.209 67 28 0 1 67 1 67 9.27e-23 87.0
MsG0580024788.01.T01 MTR_8g077540 56.364 55 22 2 18 71 17 70 1.34e-14 64.3
MsG0580024788.01.T01 MTR_8g077540 56.364 55 22 2 18 71 17 70 3.79e-14 63.9
MsG0580024788.01.T01 MTR_8g077540 56.364 55 22 2 18 71 43 96 5.04e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024788.01.T01 AT1G10630 81.818 77 14 0 1 77 1 77 8.82e-41 132
MsG0580024788.01.T01 AT1G10630 81.818 77 14 0 1 77 1 77 8.82e-41 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G23490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT1G70490 81.818 77 14 0 1 77 1 77 1.11e-40 132
MsG0580024788.01.T01 AT5G14670 81.818 77 14 0 1 77 1 77 1.83e-40 131
MsG0580024788.01.T01 AT5G14670 81.818 77 14 0 1 77 1 77 1.83e-40 131
MsG0580024788.01.T01 AT2G47170 81.818 77 14 0 1 77 1 77 2.09e-40 131
MsG0580024788.01.T01 AT2G47170 81.818 77 14 0 1 77 1 77 2.09e-40 131
MsG0580024788.01.T01 AT2G47170 81.818 77 14 0 1 77 1 77 2.09e-40 131
MsG0580024788.01.T01 AT5G14670 81.818 77 14 0 1 77 1 77 2.52e-40 131
MsG0580024788.01.T01 AT3G62290 81.818 77 14 0 1 77 1 77 2.77e-40 130
MsG0580024788.01.T01 AT3G62290 81.818 77 14 0 1 77 1 77 2.77e-40 130
MsG0580024788.01.T01 AT3G62290 81.818 77 14 0 1 77 1 77 2.77e-40 130
MsG0580024788.01.T01 AT2G15310 68.831 77 24 0 1 77 1 77 6.58e-34 115
MsG0580024788.01.T01 AT3G03120 63.636 77 28 0 1 77 1 77 1.84e-32 109
MsG0580024788.01.T01 AT3G03120 63.636 77 28 0 1 77 1 77 1.21e-31 109
MsG0580024788.01.T01 AT5G17060 62.338 77 29 0 1 77 1 77 3.69e-31 108
MsG0580024788.01.T01 AT5G17060 62.338 77 29 0 1 77 1 77 3.69e-31 108
MsG0580024788.01.T01 AT2G24765 56.250 80 34 1 1 79 1 80 2.24e-29 102
MsG0580024788.01.T01 AT2G24765 56.250 80 34 1 1 79 1 80 6.30e-29 102
MsG0580024788.01.T01 AT3G22950 58.108 74 31 0 1 74 1 74 1.78e-25 93.2
MsG0580024788.01.T01 AT3G22950 58.108 74 31 0 1 74 1 74 1.78e-25 93.2
MsG0580024788.01.T01 AT1G02430 47.222 72 37 1 1 71 1 72 7.60e-19 75.9
MsG0580024788.01.T01 AT1G02430 47.222 72 37 1 1 71 1 72 1.16e-18 75.9
MsG0580024788.01.T01 AT1G02440 42.466 73 41 1 1 72 1 73 9.81e-18 72.0
MsG0580024788.01.T01 AT1G02440 43.056 72 40 1 1 71 1 72 5.49e-17 71.6
MsG0580024788.01.T01 AT2G18390 56.364 55 22 2 18 71 17 70 6.00e-14 63.5

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGTCAGCTTCACTGTC+TGG 0.376569 5:+9700135 MsG0580024788.01.T01:CDS
AGCTTCACTGTCTGGGATGT+TGG 0.414580 5:+9700143 MsG0580024788.01.T01:CDS
TTTGGAACCTATGGTTGGTA+TGG 0.503874 5:-9699979 None:intergenic
TTTATGCAAAGAAAGAAATG+AGG 0.508896 5:+9699880 MsG0580024788.01.T01:CDS
ATACTCTACAAACTGAAGCT+TGG 0.516539 5:+9699942 MsG0580024788.01.T01:CDS
AAATGTCAGCTTCACTGTCT+GGG 0.518950 5:+9700136 MsG0580024788.01.T01:CDS
ATGGTAGGTCTTGATGCTGC+TGG 0.522575 5:+9699909 MsG0580024788.01.T01:CDS
TTCACTGTCTGGGATGTTGG+AGG 0.531178 5:+9700146 MsG0580024788.01.T01:CDS
GACTATTTGGAACCTATGGT+TGG 0.543143 5:-9699984 None:intergenic
GAAAGAAATGAGGATTCTGA+TGG 0.583316 5:+9699890 MsG0580024788.01.T01:CDS
GAAATGAGGATTCTGATGGT+AGG 0.584694 5:+9699894 MsG0580024788.01.T01:CDS
GGATGTTGGAGGACAAGACA+AGG 0.648549 5:+9700157 MsG0580024788.01.T01:CDS
AAATGGGGTTGACAGTGTCA+CGG 0.652051 5:+9699844 None:intergenic
GTCACTACCATACCAACCAT+AGG 0.654409 5:+9699972 MsG0580024788.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAGATATTATTTTTCAA+TGG - Chr5:9700009-9700028 None:intergenic 10.0%
!!! TTATTTTTCAATGGACTATT+TGG - Chr5:9700000-9700019 None:intergenic 20.0%
! TTAAAATGATCATAGGGAAT+AGG - Chr5:9700039-9700058 None:intergenic 25.0%
! TTTAATGTCTCAAATGAATC+AGG + Chr5:9700086-9700105 MsG0580024788.01.T01:intron 25.0%
! TTTATGCAAAGAAAGAAATG+AGG + Chr5:9699880-9699899 MsG0580024788.01.T01:CDS 25.0%
! TTTCGCTTAAAATGATCATA+GGG - Chr5:9700045-9700064 None:intergenic 25.0%
!! TTTTCGCTTAAAATGATCAT+AGG - Chr5:9700046-9700065 None:intergenic 25.0%
ATACTCTACAAACTGAAGCT+TGG + Chr5:9699942-9699961 MsG0580024788.01.T01:CDS 35.0%
GAAAGAAATGAGGATTCTGA+TGG + Chr5:9699890-9699909 MsG0580024788.01.T01:CDS 35.0%
!! AATGGACTATTTGGAACCTA+TGG - Chr5:9699991-9700010 None:intergenic 35.0%
AAAATGTCAGCTTCACTGTC+TGG + Chr5:9700135-9700154 MsG0580024788.01.T01:CDS 40.0%
AAATGTCAGCTTCACTGTCT+GGG + Chr5:9700136-9700155 MsG0580024788.01.T01:CDS 40.0%
GAAATGAGGATTCTGATGGT+AGG + Chr5:9699894-9699913 MsG0580024788.01.T01:CDS 40.0%
! GACTATTTGGAACCTATGGT+TGG - Chr5:9699987-9700006 None:intergenic 40.0%
! TTTGGAACCTATGGTTGGTA+TGG - Chr5:9699982-9700001 None:intergenic 40.0%
GTCACTACCATACCAACCAT+AGG + Chr5:9699972-9699991 MsG0580024788.01.T01:CDS 45.0%
ATGGTAGGTCTTGATGCTGC+TGG + Chr5:9699909-9699928 MsG0580024788.01.T01:CDS 50.0%
GGATGTTGGAGGACAAGACA+AGG + Chr5:9700157-9700176 MsG0580024788.01.T01:CDS 50.0%
! AGCTTCACTGTCTGGGATGT+TGG + Chr5:9700143-9700162 MsG0580024788.01.T01:CDS 50.0%
! TTCACTGTCTGGGATGTTGG+AGG + Chr5:9700146-9700165 MsG0580024788.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 9699846 9700208 9699846 ID=MsG0580024788.01;Name=MsG0580024788.01
Chr5 mRNA 9699846 9700208 9699846 ID=MsG0580024788.01.T01;Parent=MsG0580024788.01;Name=MsG0580024788.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|0|0|2|0|82
Chr5 exon 9699846 9699993 9699846 ID=MsG0580024788.01.T01:exon:19654;Parent=MsG0580024788.01.T01
Chr5 exon 9700108 9700208 9700108 ID=MsG0580024788.01.T01:exon:19655;Parent=MsG0580024788.01.T01
Chr5 CDS 9699846 9699993 9699846 ID=MsG0580024788.01.T01:cds;Parent=MsG0580024788.01.T01
Chr5 CDS 9700108 9700208 9700108 ID=MsG0580024788.01.T01:cds;Parent=MsG0580024788.01.T01
Gene Sequence

>MsG0580024788.01.T01

ATGGGGTTGACAGTGTCACGGATAATGAGATTATTTTATGCAAAGAAAGAAATGAGGATTCTGATGGTAGGTCTTGATGCTGCTGGAAAAACAACAATACTCTACAAACTGAAGCTTGGAGAAATTGTCACTACCATACCAACCATAGGCTTCAATGTAGAGACTGTTGAGTACAAAAATGTCAGCTTCACTGTCTGGGATGTTGGAGGACAAGACAAGGTATCATTCTTGAAATGCATTCTTTTCTAG

Protein sequence

>MsG0580024788.01.T01

MGLTVSRIMRLFYAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNVSFTVWDVGGQDKVSFLKCILF*