AlfalfaGEDB | MsG0780036550.01

M. sativa cultivar ZhongmuNo.1 / MsG0780036550.01

KEGG: K01180
GO: GO:0052861
PFAM: PF03639

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036550.01.T01	RHN44402.1	73.723	137	11	1	27	138	564	700	1.29E-57	201

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036550.01.T01	A0A396GTF3	73.723	137	11	1	27	138	564	700	6.15e-58	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048656.01	MsG0780036550.01	0.809639	1.712772e-50	1.011094e-47
MsG0180000157.01	MsG0780036550.01	0.802867	4.575097e-49	2.265790e-46
MsG0180000673.01	MsG0780036550.01	0.823936	1.059015e-53	9.248049e-51
MsG0180001009.01	MsG0780036550.01	0.805314	1.417123e-49	7.473507e-47
MsG0180001043.01	MsG0780036550.01	0.820934	5.277041e-53	4.233507e-50
MsG0180001355.01	MsG0780036550.01	0.822065	2.890979e-53	2.393925e-50
MsG0180001795.01	MsG0780036550.01	0.808000	3.840199e-50	2.170561e-47
MsG0180002482.01	MsG0780036550.01	0.813540	2.430591e-51	1.591736e-48
MsG0180003088.01	MsG0780036550.01	0.827623	1.412586e-54	1.370884e-51
MsG0180003252.01	MsG0780036550.01	0.811861	5.663374e-51	3.545076e-48
MsG0180004886.01	MsG0780036550.01	-0.869767	2.419038e-66	9.183360e-63
MsG0180005125.01	MsG0780036550.01	0.815065	1.118854e-51	7.635389e-49
MsG0180005306.01	MsG0780036550.01	0.810641	1.041743e-50	6.313709e-48
MsG0180005371.01	MsG0780036550.01	0.807677	4.496912e-50	2.520309e-47
MsG0180005664.01	MsG0780036550.01	0.801705	7.937220e-49	3.816107e-46
MsG0280006406.01	MsG0780036550.01	0.806249	9.016344e-50	4.870465e-47
MsG0280006411.01	MsG0780036550.01	0.824670	7.118584e-54	6.348510e-51
MsG0380011754.01	MsG0780036550.01	0.800672	1.291155e-48	6.047929e-46
MsG0380012661.01	MsG0780036550.01	-0.804647	1.953546e-49	1.012721e-46
MsG0680031694.01	MsG0780036550.01	0.824358	8.430714e-54	7.450142e-51
MsG0680031712.01	MsG0780036550.01	0.811894	5.571750e-51	3.490668e-48
MsG0680032641.01	MsG0780036550.01	0.811448	6.966843e-51	4.313766e-48
MsG0680035582.01	MsG0780036550.01	0.822179	2.720816e-53	2.260417e-50
MsG0480018603.01	MsG0780036550.01	0.805353	1.390653e-49	7.341261e-47
MsG0480018904.01	MsG0780036550.01	0.803046	4.202582e-49	2.090667e-46
MsG0480018906.01	MsG0780036550.01	0.804755	1.855169e-49	9.644385e-47
MsG0480020445.01	MsG0780036550.01	0.821988	3.012023e-53	2.488804e-50
MsG0480020843.01	MsG0780036550.01	0.820107	8.169512e-53	6.406020e-50
MsG0480022274.01	MsG0780036550.01	0.804273	2.338556e-49	1.200618e-46
MsG0480023517.01	MsG0780036550.01	0.805450	1.327253e-49	7.024072e-47
MsG0580024073.01	MsG0780036550.01	0.805730	1.159158e-49	6.179290e-47
MsG0580024458.01	MsG0780036550.01	-0.814496	1.495664e-51	1.005211e-48
MsG0580025134.01	MsG0780036550.01	0.800086	1.699498e-48	7.842988e-46
MsG0380015400.01	MsG0780036550.01	0.801107	1.052385e-48	4.984290e-46
MsG0380017226.01	MsG0780036550.01	0.824729	6.891506e-54	6.156314e-51
MsG0380017286.01	MsG0780036550.01	0.853551	2.217399e-61	4.809401e-58
MsG0380017680.01	MsG0780036550.01	0.801680	8.030720e-49	3.858582e-46
MsG0580027034.01	MsG0780036550.01	0.821880	3.190971e-53	2.628755e-50
MsG0580027441.01	MsG0780036550.01	0.804024	2.634289e-49	1.343917e-46
MsG0280007258.01	MsG0780036550.01	0.805713	1.169024e-49	6.228960e-47
MsG0280007609.01	MsG0780036550.01	0.817445	3.284978e-52	2.392858e-49
MsG0280010204.01	MsG0780036550.01	0.805703	1.174452e-49	6.256435e-47
MsG0280010423.01	MsG0780036550.01	-0.818364	2.036773e-52	1.521608e-49
MsG0280010493.01	MsG0780036550.01	-0.821602	3.700982e-53	3.024637e-50
MsG0780036550.01	MsG0780038444.01	0.831932	1.261321e-55	1.389075e-52
MsG0780036550.01	MsG0780038495.01	0.828087	1.092544e-54	1.074935e-51
MsG0780036550.01	MsG0780039841.01	0.802885	4.537204e-49	2.247986e-46
MsG0780036550.01	MsG0780040861.01	0.810733	9.951324e-51	6.046151e-48
MsG0780036550.01	MsG0880042611.01	0.813224	2.852863e-51	1.852069e-48
MsG0780036550.01	MsG0880043002.01	0.840317	9.352317e-58	1.327069e-54
MsG0780036550.01	MsG0880043456.01	0.802911	4.480445e-49	2.221398e-46
MsG0780036550.01	MsG0880044486.01	0.803287	3.746069e-49	1.875144e-46
MsG0780036550.01	MsG0880046058.01	0.800409	1.460927e-48	6.797655e-46
MsG0780036550.01	MsG0880046088.01	0.812776	3.576759e-51	2.294354e-48
MsG0780036550.01	MsG0880046285.01	0.809426	1.903180e-50	1.117090e-47
MsG0780036550.01	MsG0880046328.01	0.801860	7.377858e-49	3.561315e-46
MsG0780036550.01	MsG0880046731.01	0.820928	5.293151e-53	4.245744e-50
MsG0780036550.01	MsG0880046933.01	0.803230	3.849627e-49	1.924158e-46
MsG0780036550.01	MsG0880047116.01	0.804609	1.990280e-49	1.030709e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036550.01.T01	MTR_7g013810	73.723	137	11	1	27	138	512	648	2.07e-61	200
MsG0780036550.01.T01	MTR_7g013170	62.774	137	26	1	27	138	515	651	2.57e-50	170
MsG0780036550.01.T01	MTR_6g016790	49.275	138	45	2	28	140	512	649	1.42e-35	129
MsG0780036550.01.T01	MTR_6g016810	49.275	138	45	2	28	140	512	649	1.22e-34	127
MsG0780036550.01.T01	MTR_6g016795	49.635	137	44	2	29	140	519	655	1.25e-34	127
MsG0780036550.01.T01	MTR_6g016785	46.667	135	47	2	29	138	583	717	2.14e-33	124
MsG0780036550.01.T01	MTR_6g016800	47.101	138	48	4	28	140	518	655	3.63e-31	117
MsG0780036550.01.T01	MTR_6g016855	44.604	139	52	3	28	141	217	355	8.61e-30	111
MsG0780036550.01.T01	MTR_6g016820	43.165	139	53	2	29	141	521	659	8.54e-29	110
MsG0780036550.01.T01	MTR_6g016840	44.604	139	50	2	29	140	525	663	1.31e-27	107
MsG0780036550.01.T01	MTR_6g016845	43.478	138	53	3	28	140	531	668	6.06e-27	105
MsG0780036550.01.T01	MTR_1g079320	48.571	105	53	1	29	132	179	283	5.74e-26	99.8
MsG0780036550.01.T01	MTR_7g028445	41.860	129	50	1	28	131	482	610	6.66e-26	102
MsG0780036550.01.T01	MTR_4g036905	41.860	129	50	1	28	131	482	610	6.66e-26	102
MsG0780036550.01.T01	MTR_7g112730	43.220	118	51	3	36	137	170	287	2.61e-23	92.8
MsG0780036550.01.T01	MTR_6g016860	41.880	117	43	1	29	120	397	513	5.85e-20	85.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036550.01.T01	AT5G15870	41.606	137	55	1	29	140	566	702	1.18e-29	113
MsG0780036550.01.T01	AT1G18310	44.000	125	59	1	27	140	514	638	2.26e-28	109

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCGAAAATAATTTATA+TGG	0.341735	7:-9652623	MsG0780036550.01.T01:CDS
GCATTGGTTGGTTTAGCATA+TGG	0.378846	7:-9652721	MsG0780036550.01.T01:CDS
TTAGTCTTGTTCTTGAAAGA+AGG	0.411479	7:+9652411	None:intergenic
TGCTAAGGAGCTTGTGGGAA+TGG	0.430352	7:-9652439	MsG0780036550.01.T01:intron
CGCGAAAGAGAATCGGATAG+TGG	0.453096	7:-9652591	MsG0780036550.01.T01:CDS
CGACAAGATCTTGTCGCCAC+TGG	0.454351	7:-9652698	MsG0780036550.01.T01:CDS
CTAATATTGAAGGGTGGAAA+GGG	0.460560	7:-9652392	MsG0780036550.01.T01:CDS
AAATTAATGCCGTGCAAACT+TGG	0.472703	7:-9652655	MsG0780036550.01.T01:CDS
TGGAAGGAATCAAGAAAGTT+CGG	0.479501	7:-9652772	MsG0780036550.01.T01:CDS
AAGATTTCGCGAAAGAGAAT+CGG	0.480879	7:-9652598	MsG0780036550.01.T01:CDS
TACTATTCAGTAGCATTGGT+TGG	0.497731	7:-9652733	MsG0780036550.01.T01:CDS
ACTAATATTGAAGGGTGGAA+AGG	0.498150	7:-9652393	MsG0780036550.01.T01:CDS
ACAAAAGAGATAGTGCACTA+TGG	0.501201	7:-9652553	MsG0780036550.01.T01:CDS
AAGAACAAGACTAATATTGA+AGG	0.501767	7:-9652402	MsG0780036550.01.T01:CDS
CACATGCCACCAAGTTTGCA+CGG	0.502263	7:+9652646	None:intergenic
AAGAATATTAGAATGTTGAA+AGG	0.517206	7:-9652324	MsG0780036550.01.T01:CDS
CGCTAAAAGCGTTGATCCAG+TGG	0.521812	7:+9652682	None:intergenic
TGCGTACTATTCAGTAGCAT+TGG	0.524458	7:-9652737	MsG0780036550.01.T01:CDS
AGGAGCTTGTGGGAATGGAC+AGG	0.556700	7:-9652434	MsG0780036550.01.T01:CDS
GTTTATGCTAAGGAGCTTGT+GGG	0.560733	7:-9652444	MsG0780036550.01.T01:intron
GGGTTTACCTATGCTTTGCA+AGG	0.561654	7:-9652372	MsG0780036550.01.T01:CDS
AAGAGATAGTGCACTATGGT+GGG	0.565047	7:-9652549	MsG0780036550.01.T01:CDS
TGACCGAATTTGAACATGGA+AGG	0.566202	7:-9652788	None:intergenic
AAAGAGATAGTGCACTATGG+TGG	0.568637	7:-9652550	MsG0780036550.01.T01:CDS
TTAATGCCGTGCAAACTTGG+TGG	0.575009	7:-9652652	MsG0780036550.01.T01:CDS
GCGAAAGAGAATCGGATAGT+GGG	0.596944	7:-9652590	MsG0780036550.01.T01:CDS
AGAACAAGACTAATATTGAA+GGG	0.610269	7:-9652401	MsG0780036550.01.T01:CDS
TGTTTATGCTAAGGAGCTTG+TGG	0.620657	7:-9652445	MsG0780036550.01.T01:intron
ATAAACACCTTGCAAAGCAT+AGG	0.622826	7:+9652365	None:intergenic
ACAAGACTAATATTGAAGGG+TGG	0.672930	7:-9652398	MsG0780036550.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGTCGAAAATAATTTATA+TGG	-	Chr7:9652428-9652447	MsG0780036550.01.T01:CDS	15.0%
!!!	AATCTTTTAGTAATATGTTA+TGG	-	Chr7:9652763-9652782	MsG0780036550.01.T01:CDS	15.0%
!!	AAGAATATTAGAATGTTGAA+AGG	-	Chr7:9652727-9652746	MsG0780036550.01.T01:CDS	20.0%
!	AAGAACAAGACTAATATTGA+AGG	-	Chr7:9652649-9652668	MsG0780036550.01.T01:CDS	25.0%
!	AGAACAAGACTAATATTGAA+GGG	-	Chr7:9652650-9652669	MsG0780036550.01.T01:CDS	25.0%
!!	CTTTTAGTAATATGTTATGG+TGG	-	Chr7:9652766-9652785	MsG0780036550.01.T01:CDS	25.0%
	TTGAACAAAGACTCAGTTAT+AGG	+	Chr7:9652580-9652599	None:intergenic	30.0%
!!	ATGTTGAAAGGTTTTGATGA+TGG	-	Chr7:9652739-9652758	MsG0780036550.01.T01:CDS	30.0%
!!	TTAGTCTTGTTCTTGAAAGA+AGG	+	Chr7:9652643-9652662	None:intergenic	30.0%
	AAATTAATGCCGTGCAAACT+TGG	-	Chr7:9652396-9652415	MsG0780036550.01.T01:CDS	35.0%
	AAGATTTCGCGAAAGAGAAT+CGG	-	Chr7:9652453-9652472	MsG0780036550.01.T01:intron	35.0%
	ACAAAAGAGATAGTGCACTA+TGG	-	Chr7:9652498-9652517	MsG0780036550.01.T01:intron	35.0%
	ACAAGACTAATATTGAAGGG+TGG	-	Chr7:9652653-9652672	MsG0780036550.01.T01:CDS	35.0%
	ACTAATATTGAAGGGTGGAA+AGG	-	Chr7:9652658-9652677	MsG0780036550.01.T01:CDS	35.0%
	ACTGAGTCTTTGTTCAATGA+TGG	-	Chr7:9652583-9652602	MsG0780036550.01.T01:CDS	35.0%
	ATAAACACCTTGCAAAGCAT+AGG	+	Chr7:9652689-9652708	None:intergenic	35.0%
	CTAATATTGAAGGGTGGAAA+GGG	-	Chr7:9652659-9652678	MsG0780036550.01.T01:CDS	35.0%
	TGGAAGGAATCAAGAAAGTT+CGG	-	Chr7:9652279-9652298	MsG0780036550.01.T01:CDS	35.0%
!	CAATGATGGTGTTTATGCTA+AGG	-	Chr7:9652597-9652616	MsG0780036550.01.T01:CDS	35.0%
!!	TACTATTCAGTAGCATTGGT+TGG	-	Chr7:9652318-9652337	MsG0780036550.01.T01:CDS	35.0%
	AAAGAGATAGTGCACTATGG+TGG	-	Chr7:9652501-9652520	MsG0780036550.01.T01:intron	40.0%
	AAGAGATAGTGCACTATGGT+GGG	-	Chr7:9652502-9652521	MsG0780036550.01.T01:intron	40.0%
	GACTCAGTTATAGGCAACAA+AGG	+	Chr7:9652571-9652590	None:intergenic	40.0%
	GTTTATGCTAAGGAGCTTGT+GGG	-	Chr7:9652607-9652626	MsG0780036550.01.T01:CDS	40.0%
	TGCGTACTATTCAGTAGCAT+TGG	-	Chr7:9652314-9652333	MsG0780036550.01.T01:CDS	40.0%
	TGTTTATGCTAAGGAGCTTG+TGG	-	Chr7:9652606-9652625	MsG0780036550.01.T01:CDS	40.0%
!!	GCATTGGTTGGTTTAGCATA+TGG	-	Chr7:9652330-9652349	MsG0780036550.01.T01:CDS	40.0%
!!!	ATCGGATAGTGGGAATTTTG+TGG	-	Chr7:9652471-9652490	MsG0780036550.01.T01:intron	40.0%
!!!	TCGGATAGTGGGAATTTTGT+GGG	-	Chr7:9652472-9652491	MsG0780036550.01.T01:intron	40.0%
	GCGAAAGAGAATCGGATAGT+GGG	-	Chr7:9652461-9652480	MsG0780036550.01.T01:intron	45.0%
	GGGTTTACCTATGCTTTGCA+AGG	-	Chr7:9652679-9652698	MsG0780036550.01.T01:CDS	45.0%
	TTAATGCCGTGCAAACTTGG+TGG	-	Chr7:9652399-9652418	MsG0780036550.01.T01:CDS	45.0%
!	GCATCTGAATGTGAGAGTGT+AGG	-	Chr7:9652527-9652546	MsG0780036550.01.T01:intron	45.0%
!	TGGATCAACGCTTTTAGCGT+TGG	-	Chr7:9652373-9652392	MsG0780036550.01.T01:CDS	45.0%
	CACATGCCACCAAGTTTGCA+CGG	+	Chr7:9652408-9652427	None:intergenic	50.0%
	CGCGAAAGAGAATCGGATAG+TGG	-	Chr7:9652460-9652479	MsG0780036550.01.T01:intron	50.0%
	TGCTAAGGAGCTTGTGGGAA+TGG	-	Chr7:9652612-9652631	MsG0780036550.01.T01:CDS	50.0%
!!	CGCTAAAAGCGTTGATCCAG+TGG	+	Chr7:9652372-9652391	None:intergenic	50.0%
	AGGAGCTTGTGGGAATGGAC+AGG	-	Chr7:9652617-9652636	MsG0780036550.01.T01:CDS	55.0%
	CGACAAGATCTTGTCGCCAC+TGG	-	Chr7:9652353-9652372	MsG0780036550.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	9652278	9652795	9652278	ID=MsG0780036550.01;Name=MsG0780036550.01
Chr7	mRNA	9652278	9652795	9652278	ID=MsG0780036550.01.T01;Parent=MsG0780036550.01;Name=MsG0780036550.01.T01;_AED=0.39;_eAED=0.42;_QI=0\|0\|0\|1\|1\|1\|2\|0\|147
Chr7	exon	9652529	9652795	9652529	ID=MsG0780036550.01.T01:exon:1506;Parent=MsG0780036550.01.T01
Chr7	exon	9652278	9652454	9652278	ID=MsG0780036550.01.T01:exon:1505;Parent=MsG0780036550.01.T01
Chr7	CDS	9652529	9652795	9652529	ID=MsG0780036550.01.T01:cds;Parent=MsG0780036550.01.T01
Chr7	CDS	9652278	9652454	9652278	ID=MsG0780036550.01.T01:cds;Parent=MsG0780036550.01.T01

Gene Sequence

>MsG0780036550.01.T01

ATGGAAGGAATCAAGAAAGTTCGGCGAAGCAGTGAATGCGTACTATTCAGTAGCATTGGTTGGTTTAGCATATGGCGACAAGATCTTGTCGCCACTGGATCAACGCTTTTAGCGTTGGAAATTAATGCCGTGCAAACTTGGTGGCATGTGAAAGTCGAAAATAATTTATATGGTCAAGATTTCGCGAAAGAGAATCGGATAGTGGGAATTTTGTGGGCTAACAAAAGAGATAGTGCACTATGGTGGGCTGCATCTGAATGTGAGAGTGAGCTTGTGGGAATGGACAGGCCTTCTTTCAAGAACAAGACTAATATTGAAGGGTGGAAAGGGTTTACCTATGCTTTGCAAGGTGTTTATGATAAGAAGAATGCATTGAAGAATATTAGAATGTTGAAAGGTTTTGATGATGGTAATCTTTTAGTAATATGTTATGGTGGATCATAG

Protein sequence

>MsG0780036550.01.T01

MEGIKKVRRSSECVLFSSIGWFSIWRQDLVATGSTLLALEINAVQTWWHVKVENNLYGQDFAKENRIVGILWANKRDSALWWAASECESELVGMDRPSFKNKTNIEGWKGFTYALQGVYDKKNALKNIRMLKGFDDGNLLVICYGGS*