AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003252.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003252.01.T01 MTR_1g056910 96.220 291 11 0 1 291 116 406 0.0 572
MsG0180003252.01.T01 MTR_4g051515 42.756 283 155 5 6 283 123 403 3.84e-65 210
MsG0180003252.01.T01 MTR_7g106340 33.779 299 184 5 2 290 122 416 5.98e-47 162
MsG0180003252.01.T01 MTR_1g056880 36.777 242 147 4 44 284 190 426 1.91e-43 154
MsG0180003252.01.T01 MTR_1g056870 36.364 242 148 4 44 284 190 426 6.20e-41 147
MsG0180003252.01.T01 MTR_2g087350 37.063 286 169 6 1 284 119 395 1.20e-38 140
MsG0180003252.01.T01 MTR_1g056840 31.818 242 133 6 44 284 190 400 5.25e-29 114
MsG0180003252.01.T01 MTR_1g037070 45.122 82 44 1 154 235 1 81 6.98e-15 68.9
MsG0180003252.01.T01 MTR_6g462610 32.298 161 95 4 6 162 7 157 4.06e-13 66.6
MsG0180003252.01.T01 MTR_5g077510 25.939 293 192 9 7 288 155 433 4.07e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003252.01.T01 AT3G11840 36.562 320 161 4 2 283 123 438 5.83e-64 208
MsG0180003252.01.T01 AT2G35930 35.932 295 174 7 2 290 123 408 3.03e-46 160
MsG0180003252.01.T01 AT3G52450 33.992 253 154 6 43 289 185 430 3.85e-36 134

Find 31 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATTATGGAAATATTTA+AGG 0.164476 1:-59136697 MsG0180003252.01.T01:CDS
CACAATTCTTTCGAAGAATT+CGG 0.199521 1:+59137151 None:intergenic
ACTAATAGAGATTGAATTAA+TGG 0.226085 1:-59137006 MsG0180003252.01.T01:CDS
TTTACTATGTTGTTGTCCTT+TGG 0.288354 1:-59137072 MsG0180003252.01.T01:CDS
TTACTATGTTGTTGTCCTTT+GGG 0.311153 1:-59137071 MsG0180003252.01.T01:CDS
TTCTGCTACAAAGATGGTTT+TGG 0.311443 1:-59136796 MsG0180003252.01.T01:CDS
ATTTGAAAATAATCAAATAC+TGG 0.314035 1:-59137297 MsG0180003252.01.T01:CDS
GTATTTGAAAGATAAAATTA+TGG 0.351019 1:-59136709 MsG0180003252.01.T01:CDS
GTTGTCCTTTGGGAAGAAAT+AGG 0.365624 1:-59137061 MsG0180003252.01.T01:CDS
TTAAGGCTCATTCTGAGGTT+TGG 0.366681 1:-59136680 MsG0180003252.01.T01:CDS
GTTGACTGAGAGATTCTTCA+AGG 0.402733 1:-59136874 MsG0180003252.01.T01:CDS
TTGGATGAGATGTTAAAAGT+TGG 0.415505 1:-59136777 MsG0180003252.01.T01:CDS
GCTCAAGAGTTTAATGCAGT+TGG 0.418910 1:-59137501 None:intergenic
ATTGTGAAAGTTCTAAGAAA+TGG 0.433892 1:-59137134 MsG0180003252.01.T01:CDS
ACAATTCTTTCGAAGAATTC+GGG 0.437523 1:+59137152 None:intergenic
CATTTGTGTTCTTGTGCAAA+TGG 0.445659 1:-59136936 MsG0180003252.01.T01:CDS
GGGAAGAAATAGGTTAATGA+TGG 0.446476 1:-59137051 MsG0180003252.01.T01:CDS
ATGAACATAATCATCTCTTT+CGG 0.450958 1:+59137422 None:intergenic
AGAATCACAGAACTCACATT+TGG 0.479777 1:-59136969 MsG0180003252.01.T01:CDS
GAAGGTATTGAAGAAGCTCT+TGG 0.482882 1:-59137368 MsG0180003252.01.T01:CDS
AGAGATGTTTGAGGAAAACA+AGG 0.510086 1:+59136648 None:intergenic
AGATTCTTCACTTCTTCTGT+AGG 0.513088 1:+59137323 None:intergenic
AATATTTAAGGCTCATTCTG+AGG 0.547702 1:-59136685 MsG0180003252.01.T01:CDS
CTTCAAGGTTTCAATGACAG+TGG 0.556204 1:-59136859 MsG0180003252.01.T01:CDS
AAATACTGGATTCTTTAACA+TGG 0.565062 1:-59137283 MsG0180003252.01.T01:CDS
CTAGCATAGAGAGATGTTTG+AGG 0.570126 1:+59136639 None:intergenic
CTCAAACATCTCTCTATGCT+AGG 0.570560 1:-59136638 MsG0180003252.01.T01:CDS
ATTAACCTATTTCTTCCCAA+AGG 0.586930 1:+59137056 None:intergenic
AGGTTAATGATGGTTGAAGC+TGG 0.636668 1:-59137041 MsG0180003252.01.T01:CDS
GGTTACTGTGAAATCGCATG+CGG 0.706124 1:-59137234 MsG0180003252.01.T01:CDS
AATGGTGTAATAACACAACA+AGG 0.719258 1:-59137116 MsG0180003252.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATGGAAATATTTA+AGG - Chr1:59137426-59137445 MsG0180003252.01.T01:CDS 10.0%
!! ATTTGAAAATAATCAAATAC+TGG - Chr1:59136826-59136845 MsG0180003252.01.T01:CDS 15.0%
!! GTATTTGAAAGATAAAATTA+TGG - Chr1:59137414-59137433 MsG0180003252.01.T01:CDS 15.0%
!! ACTAATAGAGATTGAATTAA+TGG - Chr1:59137117-59137136 MsG0180003252.01.T01:CDS 20.0%
!! ATAGTTGATTGTTACAAAAA+AGG - Chr1:59136722-59136741 MsG0180003252.01.T01:CDS 20.0%
! AAAAAAGGCGAAATTTATGA+AGG - Chr1:59136737-59136756 MsG0180003252.01.T01:CDS 25.0%
! AAATACTGGATTCTTTAACA+TGG - Chr1:59136840-59136859 MsG0180003252.01.T01:CDS 25.0%
! ATGAACATAATCATCTCTTT+CGG + Chr1:59136704-59136723 None:intergenic 25.0%
!! AGAAAGATTTTGTTGGAAAA+TGG - Chr1:59136674-59136693 MsG0180003252.01.T01:CDS 25.0%
!! TAAATTTTCTGCTACAAAGA+TGG - Chr1:59137321-59137340 MsG0180003252.01.T01:CDS 25.0%
!!! ATTGTGAAAGTTCTAAGAAA+TGG - Chr1:59136989-59137008 MsG0180003252.01.T01:CDS 25.0%
AATGGTGTAATAACACAACA+AGG - Chr1:59137007-59137026 MsG0180003252.01.T01:CDS 30.0%
ACAATTCTTTCGAAGAATTC+GGG + Chr1:59136974-59136993 None:intergenic 30.0%
CACAATTCTTTCGAAGAATT+CGG + Chr1:59136975-59136994 None:intergenic 30.0%
TTACTATGTTGTTGTCCTTT+GGG - Chr1:59137052-59137071 MsG0180003252.01.T01:CDS 30.0%
TTGGATGAGATGTTAAAAGT+TGG - Chr1:59137346-59137365 MsG0180003252.01.T01:CDS 30.0%
TTTACTATGTTGTTGTCCTT+TGG - Chr1:59137051-59137070 MsG0180003252.01.T01:CDS 30.0%
! AATATTTAAGGCTCATTCTG+AGG - Chr1:59137438-59137457 MsG0180003252.01.T01:CDS 30.0%
! ATTAACCTATTTCTTCCCAA+AGG + Chr1:59137070-59137089 None:intergenic 30.0%
! GAGCAATAGAAAGATTTTGT+TGG - Chr1:59136667-59136686 MsG0180003252.01.T01:CDS 30.0%
!!! TTTCAGTTTTTGAGTCATCA+AGG - Chr1:59137214-59137233 MsG0180003252.01.T01:CDS 30.0%
AGAATCACAGAACTCACATT+TGG - Chr1:59137154-59137173 MsG0180003252.01.T01:CDS 35.0%
AGAGATGTTTGAGGAAAACA+AGG + Chr1:59137478-59137497 None:intergenic 35.0%
AGATTCTTCACTTCTTCTGT+AGG + Chr1:59136803-59136822 None:intergenic 35.0%
AGTTTCCGAGATGAAAAACT+CGG - Chr1:59136868-59136887 MsG0180003252.01.T01:CDS 35.0%
CATTTGTGTTCTTGTGCAAA+TGG - Chr1:59137187-59137206 MsG0180003252.01.T01:CDS 35.0%
GGGAAGAAATAGGTTAATGA+TGG - Chr1:59137072-59137091 MsG0180003252.01.T01:CDS 35.0%
! AGTAACCGAGTTTTTCATCT+CGG + Chr1:59136876-59136895 None:intergenic 35.0%
! TTCTGCTACAAAGATGGTTT+TGG - Chr1:59137327-59137346 MsG0180003252.01.T01:CDS 35.0%
AGGTTAATGATGGTTGAAGC+TGG - Chr1:59137082-59137101 MsG0180003252.01.T01:CDS 40.0%
CTAGCATAGAGAGATGTTTG+AGG + Chr1:59137487-59137506 None:intergenic 40.0%
CTCAAACATCTCTCTATGCT+AGG - Chr1:59137485-59137504 MsG0180003252.01.T01:CDS 40.0%
CTTCAAGGTTTCAATGACAG+TGG - Chr1:59137264-59137283 MsG0180003252.01.T01:CDS 40.0%
GTTGACTGAGAGATTCTTCA+AGG - Chr1:59137249-59137268 MsG0180003252.01.T01:CDS 40.0%
! GTTGTCCTTTGGGAAGAAAT+AGG - Chr1:59137062-59137081 MsG0180003252.01.T01:CDS 40.0%
!! GAAGGTATTGAAGAAGCTCT+TGG - Chr1:59136755-59136774 MsG0180003252.01.T01:CDS 40.0%
!! TTAAGGCTCATTCTGAGGTT+TGG - Chr1:59137443-59137462 MsG0180003252.01.T01:CDS 40.0%
!!! GCGATTCAATCGTTTTAGAG+AGG - Chr1:59136942-59136961 MsG0180003252.01.T01:CDS 40.0%
GGTTACTGTGAAATCGCATG+CGG - Chr1:59136889-59136908 MsG0180003252.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 59136635 59137510 59136635 ID=MsG0180003252.01;Name=MsG0180003252.01
Chr1 mRNA 59136635 59137510 59136635 ID=MsG0180003252.01.T01;Parent=MsG0180003252.01;Name=MsG0180003252.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|291
Chr1 exon 59136635 59137510 59136635 ID=MsG0180003252.01.T01:exon:35026;Parent=MsG0180003252.01.T01
Chr1 CDS 59136635 59137510 59136635 ID=MsG0180003252.01.T01:cds;Parent=MsG0180003252.01.T01
Gene Sequence

>MsG0180003252.01.T01

ATGCAGTTGGAATTGCTTGCTGCTGAGAAAGAGAGCAATAGAAAGATTTTGTTGGAAAATGGTGTGCCGAAAGAGATGATTATGTTCATAGTTGATTGTTACAAAAAAGGCGAAATTTATGAAGGTATTGAAGAAGCTCTTGGTTTTTTACAATTTGTTAAAGTTCCTACAGAAGAAGTGAAGAATCTTCTATTTGAAAATAATCAAATACTGGATTCTTTAACATGGATTTTAGTTTCCGAGATGAAAAACTCGGTTACTGTGAAATCGCATGCGGTTTTAGTGCTTAAAAAATTCATAAACAAATGCGATTCAATCGTTTTAGAGAGGCTAAAACCCGAATTCTTCGAAAGAATTGTGAAAGTTCTAAGAAATGGTGTAATAACACAACAAGGTTTGTCTTCTGCTTTACATATTTTACTATGTTGTTGTCCTTTGGGAAGAAATAGGTTAATGATGGTTGAAGCTGGTGCAATTCATGAACTAATAGAGATTGAATTAATGGCAATACATGAAAAGAGAATCACAGAACTCACATTTGGTATATTGTTTCATTTGTGTTCTTGTGCAAATGGAAGATTTCAGTTTTTGAGTCATCAAGGTTCAATTGCAATGTTGACTGAGAGATTCTTCAAGGTTTCAATGACAGTGGATGATAGAGCTGTTTTTATACTCTCTTTGATATCTAAATTTTCTGCTACAAAGATGGTTTTGGATGAGATGTTAAAAGTTGGAACTGTTGCAAAGCTTTTTGTTTTGCTTCAAAGTGATCATGCTAAGTATTTGAAAGATAAAATTATGGAAATATTTAAGGCTCATTCTGAGGTTTGGAAGAATTCTCCTTGTTTTCCTCAAACATCTCTCTATGCTAGGTGA

Protein sequence

>MsG0180003252.01.T01

MQLELLAAEKESNRKILLENGVPKEMIMFIVDCYKKGEIYEGIEEALGFLQFVKVPTEEVKNLLFENNQILDSLTWILVSEMKNSVTVKSHAVLVLKKFINKCDSIVLERLKPEFFERIVKVLRNGVITQQGLSSALHILLCCCPLGRNRLMMVEAGAIHELIEIELMAIHEKRITELTFGILFHLCSCANGRFQFLSHQGSIAMLTERFFKVSMTVDDRAVFILSLISKFSATKMVLDEMLKVGTVAKLFVLLQSDHAKYLKDKIMEIFKAHSEVWKNSPCFPQTSLYAR*