AlfalfaGEDB | MsG0180005306.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005306.01

KEGG: K14412
GO: GO:0006486 GO:0008417 GO:0016020
PFAM: PF00852

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005306.01.T01	GAU15969.1	98.734	79	1	0	1	79	143	221	7.48E-50	166

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005306.01.T01	Q9C8W3	78.481	79	17	0	1	79	308	386	2.51E-41	141

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005306.01.T01	A0A4D6N688	96.203	79	3	0	1	79	308	386	1.38e-47	171
MsG0180005306.01.T01	Q93WR9	100.000	79	0	0	1	79	189	267	3.76e-50	167

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0180005306.01	0.802346	5.860244e-49	2.864018e-46
MsG0080047825.01	MsG0180005306.01	0.813061	3.097043e-51	2.001809e-48
MsG0080048968.01	MsG0180005306.01	0.818639	1.764407e-52	1.328015e-49
MsG0180000055.01	MsG0180005306.01	0.823796	1.142025e-53	9.934117e-51
MsG0180000157.01	MsG0180005306.01	0.816831	4.515937e-52	3.234425e-49
MsG0180000235.01	MsG0180005306.01	0.803737	3.022714e-49	1.530709e-46
MsG0180000550.01	MsG0180005306.01	0.819883	9.192919e-53	7.162643e-50
MsG0180000585.01	MsG0180005306.01	0.801609	8.305045e-49	3.983225e-46
MsG0180000822.01	MsG0180005306.01	0.839474	1.551077e-57	2.144385e-54
MsG0180001009.01	MsG0180005306.01	0.810372	1.191183e-50	7.167585e-48
MsG0180001043.01	MsG0180005306.01	0.841849	3.702830e-58	5.511116e-55
MsG0180001355.01	MsG0180005306.01	0.825104	5.622271e-54	5.076219e-51
MsG0180001921.01	MsG0180005306.01	0.836699	8.030264e-57	1.019951e-53
MsG0180002075.01	MsG0180005306.01	0.867669	1.153948e-65	4.061871e-62
MsG0180002750.01	MsG0180005306.01	-0.807504	4.894565e-50	2.730958e-47
MsG0180002894.01	MsG0180005306.01	0.803082	4.131511e-49	2.057172e-46
MsG0180004128.01	MsG0180005306.01	0.810509	1.112595e-50	6.719148e-48
MsG0180004388.01	MsG0180005306.01	0.821981	3.024001e-53	2.498167e-50
MsG0180004767.01	MsG0180005306.01	0.804460	2.137940e-49	1.102927e-46
MsG0180004824.01	MsG0180005306.01	0.829996	3.766196e-55	3.917667e-52
MsG0180004843.01	MsG0180005306.01	0.808918	2.445242e-50	1.415801e-47
MsG0180004886.01	MsG0180005306.01	-0.810638	1.043704e-50	6.324803e-48
MsG0180004991.01	MsG0180005306.01	0.809102	2.233332e-50	1.299588e-47
MsG0180005125.01	MsG0180005306.01	0.812220	4.731557e-51	2.990921e-48
MsG0180005233.01	MsG0180005306.01	0.833153	6.279613e-56	7.170650e-53
MsG0180005268.01	MsG0180005306.01	0.821223	4.526492e-53	3.660798e-50
MsG0180005306.01	MsG0180005664.01	0.812119	4.977195e-51	3.137361e-48
MsG0180005306.01	MsG0180005763.01	0.807317	5.361028e-50	2.976774e-47
MsG0180005306.01	MsG0180005944.01	0.834806	2.422537e-56	2.906433e-53
MsG0180005306.01	MsG0180006092.01	0.802846	4.622178e-49	2.287921e-46
MsG0180005306.01	MsG0280006302.01	-0.834110	3.621316e-56	4.255419e-53
MsG0180005306.01	MsG0280006406.01	0.816002	6.922012e-52	4.846642e-49
MsG0180005306.01	MsG0280007440.01	0.817866	2.640261e-52	1.945457e-49
MsG0180005306.01	MsG0280007441.01	0.803184	3.935526e-49	1.964822e-46
MsG0180005306.01	MsG0280007547.01	0.812050	5.151550e-51	3.241161e-48
MsG0180005306.01	MsG0280007609.01	0.819845	9.377603e-53	7.298826e-50
MsG0180005306.01	MsG0280007710.01	0.811159	8.047988e-51	4.945077e-48
MsG0180005306.01	MsG0280007764.01	0.803671	3.119464e-49	1.576945e-46
MsG0180005306.01	MsG0280008104.01	0.819416	1.174618e-52	9.033587e-50
MsG0180005306.01	MsG0280008239.01	0.829686	4.480368e-55	4.619228e-52
MsG0180005306.01	MsG0280008295.01	0.808744	2.664257e-50	1.535533e-47
MsG0180005306.01	MsG0280009235.01	0.801549	8.545518e-49	4.092369e-46
MsG0180005306.01	MsG0280010170.01	0.803308	3.708426e-49	1.857311e-46
MsG0180005306.01	MsG0280010739.01	0.800769	1.233509e-48	5.791841e-46
MsG0180005306.01	MsG0280011141.01	0.811309	7.467468e-51	4.606414e-48
MsG0180005306.01	MsG0380011754.01	0.801721	7.876724e-49	3.788556e-46
MsG0180005306.01	MsG0380012186.01	0.832560	8.818137e-56	9.892348e-53
MsG0180005306.01	MsG0380012187.01	0.803558	3.292484e-49	1.659766e-46
MsG0180005306.01	MsG0380014680.01	0.808986	2.364644e-50	1.371657e-47
MsG0180005306.01	MsG0380014908.01	0.819175	1.333108e-52	1.018378e-49
MsG0180005306.01	MsG0380015779.01	0.813315	2.723365e-51	1.772541e-48
MsG0180005306.01	MsG0380016859.01	0.820773	5.744958e-53	4.588177e-50
MsG0180005306.01	MsG0380017286.01	0.838121	3.471483e-57	4.604501e-54
MsG0180005306.01	MsG0380017375.01	0.800214	1.601054e-48	7.412636e-46
MsG0180005306.01	MsG0380017604.01	0.809265	2.061263e-50	1.204666e-47
MsG0180005306.01	MsG0380017631.01	0.828222	1.013533e-54	1.001113e-51
MsG0180005306.01	MsG0380017680.01	0.805898	1.068773e-49	5.721651e-47
MsG0180005306.01	MsG0380017888.01	0.813662	2.285319e-51	1.501507e-48
MsG0180005306.01	MsG0380017941.01	0.807069	6.050680e-50	3.337931e-47
MsG0180005306.01	MsG0480018635.01	0.804661	1.941193e-49	1.006649e-46
MsG0180005306.01	MsG0480018640.01	0.838411	2.922949e-57	3.911701e-54
MsG0180005306.01	MsG0480018787.01	0.805990	1.022084e-49	5.484805e-47
MsG0180005306.01	MsG0480018970.01	0.818056	2.391988e-52	1.771669e-49
MsG0180005306.01	MsG0480020445.01	0.807953	3.929735e-50	2.218347e-47
MsG0180005306.01	MsG0480020720.01	0.809736	1.633026e-50	9.664522e-48
MsG0180005306.01	MsG0480020843.01	0.802719	4.909674e-49	2.422334e-46
MsG0180005306.01	MsG0480021061.01	0.816553	5.212884e-52	3.705190e-49
MsG0180005306.01	MsG0480022035.01	0.805087	1.581238e-49	8.290515e-47
MsG0180005306.01	MsG0480022451.01	0.811978	5.342184e-51	3.354563e-48
MsG0180005306.01	MsG0480022480.01	0.817105	3.917725e-52	2.827154e-49
MsG0180005306.01	MsG0480022791.01	0.811698	6.147406e-51	3.831245e-48
MsG0180005306.01	MsG0480023245.01	0.802566	5.278286e-49	2.593962e-46
MsG0180005306.01	MsG0480023361.01	0.802846	4.621555e-49	2.287648e-46
MsG0180005306.01	MsG0480023412.01	0.809242	2.084462e-50	1.217523e-47
MsG0180005306.01	MsG0480023517.01	0.810504	1.115518e-50	6.735931e-48
MsG0180005306.01	MsG0480023554.01	0.819990	8.688943e-53	6.790322e-50
MsG0180005306.01	MsG0580024114.01	0.814957	1.182595e-51	8.046709e-49
MsG0180005306.01	MsG0580025328.01	0.807797	4.240124e-50	2.383875e-47
MsG0180005306.01	MsG0580025788.01	-0.805461	1.320215e-49	6.988967e-47
MsG0180005306.01	MsG0580025799.01	0.809480	1.853214e-50	1.089289e-47
MsG0180005306.01	MsG0580027034.01	0.808519	2.976137e-50	1.705315e-47
MsG0180005306.01	MsG0580027441.01	0.844767	6.164709e-59	1.005653e-55
MsG0180005306.01	MsG0580029594.01	0.801050	1.081242e-48	5.113564e-46
MsG0180005306.01	MsG0580029629.01	0.819007	1.455654e-52	1.107003e-49
MsG0180005306.01	MsG0580029762.01	0.807912	4.008811e-50	2.260459e-47
MsG0180005306.01	MsG0580030204.01	0.828193	1.029960e-54	1.016493e-51
MsG0180005306.01	MsG0580030235.01	0.827022	1.968115e-54	1.877014e-51
MsG0180005306.01	MsG0680030685.01	0.824103	9.673742e-54	8.487144e-51
MsG0180005306.01	MsG0680031459.01	0.837085	6.401532e-57	8.225077e-54
MsG0180005306.01	MsG0680031538.01	0.824648	7.204944e-54	6.421203e-51
MsG0180005306.01	MsG0680031651.01	0.802235	6.176397e-49	3.009914e-46
MsG0180005306.01	MsG0680034655.01	0.816729	4.759715e-52	3.399495e-49
MsG0180005306.01	MsG0680035582.01	0.807350	5.276015e-50	2.931957e-47
MsG0180005306.01	MsG0680035844.01	0.811362	7.272620e-51	4.492554e-48
MsG0180005306.01	MsG0680035854.01	0.832525	8.996529e-56	1.008123e-52
MsG0180005306.01	MsG0780036240.01	0.813645	2.304362e-51	1.513343e-48
MsG0180005306.01	MsG0780036550.01	0.810641	1.041743e-50	6.313709e-48
MsG0180005306.01	MsG0780036609.01	0.822141	2.776234e-53	2.304081e-50
MsG0180005306.01	MsG0780038443.01	0.814720	1.334476e-51	9.022716e-49
MsG0180005306.01	MsG0780038495.01	0.824718	6.934423e-54	6.192798e-51
MsG0180005306.01	MsG0780039943.01	0.803807	2.922478e-49	1.482592e-46
MsG0180005306.01	MsG0780040718.01	0.806838	6.773658e-50	3.714537e-47
MsG0180005306.01	MsG0780040726.01	0.817682	2.905345e-52	2.130001e-49
MsG0180005306.01	MsG0780040861.01	0.807851	4.129266e-50	2.324757e-47
MsG0180005306.01	MsG0780041192.01	0.822010	2.977273e-53	2.461616e-50
MsG0180005306.01	MsG0780041300.01	0.812041	5.176193e-51	3.255693e-48
MsG0180005306.01	MsG0780041351.01	0.803141	4.016647e-49	2.003088e-46
MsG0180005306.01	MsG0780041399.01	0.806950	6.413886e-50	3.527358e-47
MsG0180005306.01	MsG0780041469.01	0.806669	7.353264e-50	4.014545e-47
MsG0180005306.01	MsG0880042053.01	0.821743	3.432687e-53	2.816672e-50
MsG0180005306.01	MsG0880042107.01	0.809095	2.241672e-50	1.304162e-47
MsG0180005306.01	MsG0880042543.01	0.826509	2.608924e-54	2.451648e-51
MsG0180005306.01	MsG0880043002.01	0.803496	3.390429e-49	1.706365e-46
MsG0180005306.01	MsG0880044486.01	0.803248	3.816870e-49	1.908700e-46
MsG0180005306.01	MsG0880045106.01	0.813049	3.115192e-51	2.012922e-48
MsG0180005306.01	MsG0880045756.01	0.803092	4.112020e-49	2.047959e-46
MsG0180005306.01	MsG0880046091.01	0.821152	4.698994e-53	3.792724e-50
MsG0180005306.01	MsG0880046244.01	0.804069	2.578106e-49	1.316816e-46
MsG0180005306.01	MsG0880046328.01	0.802715	4.917799e-49	2.426146e-46
MsG0180005306.01	MsG0880046731.01	0.821302	4.339679e-53	3.517240e-50
MsG0180005306.01	MsG0880046933.01	0.808179	3.517016e-50	1.997399e-47
MsG0180005306.01	MsG0880046973.01	0.806425	8.276146e-50	4.490620e-47
MsG0180005306.01	MsG0880047120.01	0.812027	5.212931e-51	3.277681e-48
MsG0180005306.01	MsG0880047143.01	0.812146	4.909338e-51	3.097037e-48
MsG0180005306.01	MsG0880047192.01	0.800309	1.530999e-48	7.105821e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005306.01.T01	MTR_1g096880	100.000	79	0	0	1	79	319	397	1.90e-52	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005306.01.T01	AT1G71990	78.481	79	17	0	1	79	308	386	2.56e-42	141

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGCAGTTCCTATCTATTT+TGG	0.214861	1:-89995127	MsG0180005306.01.T01:CDS
TGGTATTCTGCATAAGCTAC+TGG	0.386380	1:+89994998	None:intergenic
ATTCAAATCAGTGGAAGAAC+TGG	0.415680	1:-89995050	MsG0180005306.01.T01:CDS
CCTCCACATTCAATAATAGA+TGG	0.430995	1:-89995079	MsG0180005306.01.T01:CDS
GGAACAAAATCCATGACGTT+TGG	0.472091	1:+89995100	None:intergenic
ATGGAGACGGTGCGGTGTAA+TGG	0.473423	1:-89994972	MsG0180005306.01.T01:CDS
TGGAGACGGTGCGGTGTAAT+GGG	0.478468	1:-89994971	MsG0180005306.01.T01:CDS
CTACCATCTATTATTGAATG+TGG	0.490630	1:+89995076	None:intergenic
TTTGGTGCACCAAAATAGAT+AGG	0.511615	1:+89995118	None:intergenic
AGCTACTGGGTCATTAGCTA+CGG	0.551807	1:+89995012	None:intergenic
AACGGCATGGCAATGTGTCT+AGG	0.555334	1:+89994921	None:intergenic
CACCGCACCGTCTCCATGCA+TGG	0.557704	1:+89994978	None:intergenic
GGTATTCTGCATAAGCTACT+GGG	0.564653	1:+89994999	None:intergenic
TGGTAGAAAATTCAAATCAG+TGG	0.588336	1:-89995059	MsG0180005306.01.T01:CDS
CAGAATACCATGCATGGAGA+CGG	0.596551	1:-89994985	MsG0180005306.01.T01:CDS
CTTATGCAGAATACCATGCA+TGG	0.607593	1:-89994991	MsG0180005306.01.T01:CDS
AGAACTGGCTTCCTACGTGA+AGG	0.625991	1:-89995035	MsG0180005306.01.T01:CDS
CCATCTATTATTGAATGTGG+AGG	0.630669	1:+89995079	None:intergenic
TACCATGCATGGAGACGGTG+CGG	0.663582	1:-89994980	MsG0180005306.01.T01:CDS
ATTAGCTACGGCCTTCACGT+AGG	0.699128	1:+89995024	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTACCATCTATTATTGAATG+TGG	+	Chr1:89994982-89995001	None:intergenic	30.0%
	TGGTAGAAAATTCAAATCAG+TGG	-	Chr1:89994996-89995015	MsG0180005306.01.T01:CDS	30.0%
	ATTCAAATCAGTGGAAGAAC+TGG	-	Chr1:89995005-89995024	MsG0180005306.01.T01:CDS	35.0%
	CCATCTATTATTGAATGTGG+AGG	+	Chr1:89994979-89994998	None:intergenic	35.0%
	CCTCCACATTCAATAATAGA+TGG	-	Chr1:89994976-89994995	MsG0180005306.01.T01:CDS	35.0%
!	TTTGGTGCACCAAAATAGAT+AGG	+	Chr1:89994940-89994959	None:intergenic	35.0%
	CTTATGCAGAATACCATGCA+TGG	-	Chr1:89995064-89995083	MsG0180005306.01.T01:CDS	40.0%
	GGAACAAAATCCATGACGTT+TGG	+	Chr1:89994958-89994977	None:intergenic	40.0%
	GGTATTCTGCATAAGCTACT+GGG	+	Chr1:89995059-89995078	None:intergenic	40.0%
	TGGTATTCTGCATAAGCTAC+TGG	+	Chr1:89995060-89995079	None:intergenic	40.0%
!	GGTGCAGTTCCTATCTATTT+TGG	-	Chr1:89994928-89994947	MsG0180005306.01.T01:CDS	40.0%
	AGCTACTGGGTCATTAGCTA+CGG	+	Chr1:89995046-89995065	None:intergenic	45.0%
	CAGAATACCATGCATGGAGA+CGG	-	Chr1:89995070-89995089	MsG0180005306.01.T01:CDS	45.0%
!!	TTTTGGTGCACCAAACGTCA+TGG	-	Chr1:89994945-89994964	MsG0180005306.01.T01:CDS	45.0%
	AACGGCATGGCAATGTGTCT+AGG	+	Chr1:89995137-89995156	None:intergenic	50.0%
	ATTAGCTACGGCCTTCACGT+AGG	+	Chr1:89995034-89995053	None:intergenic	50.0%
!	AGAACTGGCTTCCTACGTGA+AGG	-	Chr1:89995020-89995039	MsG0180005306.01.T01:CDS	50.0%
	ATGGAGACGGTGCGGTGTAA+TGG	-	Chr1:89995083-89995102	MsG0180005306.01.T01:CDS	55.0%
	TACCATGCATGGAGACGGTG+CGG	-	Chr1:89995075-89995094	MsG0180005306.01.T01:CDS	55.0%
	TGGAGACGGTGCGGTGTAAT+GGG	-	Chr1:89995084-89995103	MsG0180005306.01.T01:CDS	55.0%
	CACCGCACCGTCTCCATGCA+TGG	+	Chr1:89995080-89995099	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	89994919	89995158	89994919	ID=MsG0180005306.01;Name=MsG0180005306.01
Chr1	mRNA	89994919	89995158	89994919	ID=MsG0180005306.01.T01;Parent=MsG0180005306.01;Name=MsG0180005306.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|79
Chr1	exon	89994919	89995158	89994919	ID=MsG0180005306.01.T01:exon:47938;Parent=MsG0180005306.01.T01
Chr1	CDS	89994919	89995158	89994919	ID=MsG0180005306.01.T01:cds;Parent=MsG0180005306.01.T01

Gene Sequence	>MsG0180005306.01.T01 TTGGACTCGGGTGCAGTTCCTATCTATTTTGGTGCACCAAACGTCATGGATTTTGTTCCTCCACATTCAATAATAGATGGTAGAAAATTCAAATCAGTGGAAGAACTGGCTTCCTACGTGAAGGCCGTAGCTAATGACCCAGTAGCTTATGCAGAATACCATGCATGGAGACGGTGCGGTGTAATGGGTAACTACGCGAAAACACGAGCTATGAGCCTAGACACATTGCCATGCCGTTAG
Protein sequence	>MsG0180005306.01.T01 LDSGAVPIYFGAPNVMDFVPPHSIIDGRKFKSVEELASYVKAVANDPVAYAEYHAWRRCGVMGNYAKTRAMSLDTLPCR*