Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041232.01.T01 | KEH24315.1 | 95.455 | 88 | 4 | 0 | 1 | 88 | 66 | 153 | 3.14E-42 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041232.01.T01 | A0A072UEV4 | 95.455 | 88 | 4 | 0 | 1 | 88 | 66 | 153 | 1.50e-42 | 149 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048738.01 | MsG0780041232.01 | 0.835345 | 1.771662e-56 | 2.160639e-53 |
| MsG0080048797.01 | MsG0780041232.01 | 0.839333 | 1.687097e-57 | 2.322417e-54 |
| MsG0180001293.01 | MsG0780041232.01 | 0.807252 | 5.536153e-50 | 3.068688e-47 |
| MsG0180001719.01 | MsG0780041232.01 | 0.837621 | 4.665309e-57 | 6.094940e-54 |
| MsG0180002134.01 | MsG0780041232.01 | 0.816654 | 4.947530e-52 | 3.526593e-49 |
| MsG0180002135.01 | MsG0780041232.01 | 0.815571 | 8.633365e-52 | 5.974219e-49 |
| MsG0180002428.01 | MsG0780041232.01 | 0.800632 | 1.316015e-48 | 6.157954e-46 |
| MsG0180003857.01 | MsG0780041232.01 | 0.801925 | 7.153479e-49 | 3.458601e-46 |
| MsG0180003905.01 | MsG0780041232.01 | 0.862245 | 5.808270e-64 | 1.689640e-60 |
| MsG0180004120.01 | MsG0780041232.01 | 0.849864 | 2.458871e-60 | 4.726689e-57 |
| MsG0180004185.01 | MsG0780041232.01 | 0.803082 | 4.131505e-49 | 2.057171e-46 |
| MsG0180004538.01 | MsG0780041232.01 | 0.807497 | 4.911318e-50 | 2.739780e-47 |
| MsG0180004583.01 | MsG0780041232.01 | 0.822172 | 2.730876e-53 | 2.268306e-50 |
| MsG0180004828.01 | MsG0780041232.01 | 0.825141 | 5.510609e-54 | 4.980959e-51 |
| MsG0180005655.01 | MsG0780041232.01 | 0.846018 | 2.826004e-59 | 4.798934e-56 |
| MsG0180005819.01 | MsG0780041232.01 | 0.810890 | 9.202337e-51 | 5.614767e-48 |
| MsG0180006183.01 | MsG0780041232.01 | 0.819358 | 1.211231e-52 | 9.299324e-50 |
| MsG0780040554.01 | MsG0780041232.01 | 0.837736 | 4.358933e-57 | 5.715007e-54 |
| MsG0780040641.01 | MsG0780041232.01 | 0.810295 | 1.237433e-50 | 7.430737e-48 |
| MsG0780040899.01 | MsG0780041232.01 | 0.865356 | 6.267339e-65 | 2.032995e-61 |
| MsG0780040978.01 | MsG0780041232.01 | 0.839451 | 1.572075e-57 | 2.171966e-54 |
| MsG0780041219.01 | MsG0780041232.01 | 0.824192 | 9.221206e-54 | 8.110611e-51 |
| MsG0780041232.01 | MsG0780041273.01 | 0.820282 | 7.448206e-53 | 5.867998e-50 |
| MsG0780041232.01 | MsG0780041560.01 | 0.829274 | 5.641097e-55 | 5.746836e-52 |
| MsG0780041232.01 | MsG0880042392.01 | 0.805238 | 1.470456e-49 | 7.739496e-47 |
| MsG0780041232.01 | MsG0880044437.01 | 0.829994 | 3.769659e-55 | 3.921095e-52 |
| MsG0780041232.01 | MsG0880045356.01 | 0.849697 | 2.738622e-60 | 5.235631e-57 |
| MsG0780041232.01 | MsG0880045439.01 | 0.812643 | 3.824701e-51 | 2.444951e-48 |
| MsG0780041232.01 | MsG0880045647.01 | 0.848589 | 5.568138e-60 | 1.026809e-56 |
| MsG0780041232.01 | MsG0880046904.01 | 0.803321 | 3.686416e-49 | 1.846885e-46 |
| MsG0780041232.01 | MsG0880047057.01 | 0.801147 | 1.032852e-48 | 4.896557e-46 |
| MsG0780041232.01 | MsG0880047414.01 | 0.807555 | 4.773930e-50 | 2.667034e-47 |
| MsG0380014542.01 | MsG0780041232.01 | 0.830234 | 3.293981e-55 | 3.450265e-52 |
| MsG0380014544.01 | MsG0780041232.01 | 0.831288 | 1.817014e-55 | 1.962923e-52 |
| MsG0380014666.01 | MsG0780041232.01 | 0.833813 | 4.299772e-56 | 5.007904e-53 |
| MsG0680031356.01 | MsG0780041232.01 | 0.828680 | 7.858006e-55 | 7.865613e-52 |
| MsG0480018486.01 | MsG0780041232.01 | 0.818263 | 2.147141e-52 | 1.599392e-49 |
| MsG0480020398.01 | MsG0780041232.01 | 0.888263 | 6.894017e-73 | 5.302705e-69 |
| MsG0480020856.01 | MsG0780041232.01 | 0.812718 | 3.682004e-51 | 2.358440e-48 |
| MsG0480020937.01 | MsG0780041232.01 | 0.840889 | 6.623344e-58 | 9.568025e-55 |
| MsG0480020970.01 | MsG0780041232.01 | 0.810404 | 1.172430e-50 | 7.060495e-48 |
| MsG0480021100.01 | MsG0780041232.01 | 0.838158 | 3.395932e-57 | 4.509315e-54 |
| MsG0480021301.01 | MsG0780041232.01 | 0.809175 | 2.154762e-50 | 1.256294e-47 |
| MsG0480021773.01 | MsG0780041232.01 | 0.851625 | 7.857509e-61 | 1.599930e-57 |
| MsG0480021902.01 | MsG0780041232.01 | 0.822982 | 1.769587e-53 | 1.504252e-50 |
| MsG0480022307.01 | MsG0780041232.01 | 0.832022 | 1.197886e-55 | 1.322612e-52 |
| MsG0480022378.01 | MsG0780041232.01 | 0.810665 | 1.029441e-50 | 6.243316e-48 |
| MsG0480023414.01 | MsG0780041232.01 | 0.873771 | 1.135669e-67 | 4.987686e-64 |
| MsG0480023424.01 | MsG0780041232.01 | 0.802955 | 4.388285e-49 | 2.178058e-46 |
| MsG0480023426.01 | MsG0780041232.01 | 0.852075 | 5.857402e-61 | 1.209841e-57 |
| MsG0480023706.01 | MsG0780041232.01 | 0.843548 | 1.309708e-58 | 2.056229e-55 |
| MsG0480023842.01 | MsG0780041232.01 | 0.839702 | 1.352795e-57 | 1.883232e-54 |
| MsG0480023913.01 | MsG0780041232.01 | 0.804719 | 1.887217e-49 | 9.801969e-47 |
| MsG0480024019.01 | MsG0780041232.01 | 0.824451 | 8.013480e-54 | 7.100245e-51 |
| MsG0580024276.01 | MsG0780041232.01 | 0.827751 | 1.316181e-54 | 1.282206e-51 |
| MsG0580025504.01 | MsG0780041232.01 | 0.837834 | 4.112817e-57 | 5.408460e-54 |
| MsG0380015340.01 | MsG0780041232.01 | 0.837870 | 4.026250e-57 | 5.300364e-54 |
| MsG0380015402.01 | MsG0780041232.01 | 0.821180 | 4.629610e-53 | 3.739781e-50 |
| MsG0380015656.01 | MsG0780041232.01 | 0.826041 | 3.372332e-54 | 3.127085e-51 |
| MsG0380015954.01 | MsG0780041232.01 | 0.808072 | 3.706175e-50 | 2.098755e-47 |
| MsG0380016250.01 | MsG0780041232.01 | 0.836095 | 1.143495e-56 | 1.426337e-53 |
| MsG0380017128.01 | MsG0780041232.01 | 0.852625 | 4.082565e-61 | 8.581986e-58 |
| MsG0380017332.01 | MsG0780041232.01 | 0.829780 | 4.251460e-55 | 4.395484e-52 |
| MsG0380017588.01 | MsG0780041232.01 | 0.854160 | 1.481153e-61 | 3.278317e-58 |
| MsG0380017696.01 | MsG0780041232.01 | 0.849274 | 3.591826e-60 | 6.773732e-57 |
| MsG0380017932.01 | MsG0780041232.01 | 0.817544 | 3.121363e-52 | 2.279825e-49 |
| MsG0480018228.01 | MsG0780041232.01 | 0.872045 | 4.300270e-67 | 1.773115e-63 |
| MsG0580026343.01 | MsG0780041232.01 | 0.843538 | 1.317925e-58 | 2.068484e-55 |
| MsG0580026446.01 | MsG0780041232.01 | 0.806334 | 8.653294e-50 | 4.684279e-47 |
| MsG0580028927.01 | MsG0780041232.01 | 0.810910 | 9.114986e-51 | 5.564282e-48 |
| MsG0580028955.01 | MsG0780041232.01 | 0.829992 | 3.774351e-55 | 3.925717e-52 |
| MsG0580030141.01 | MsG0780041232.01 | 0.851366 | 9.300664e-61 | 1.878378e-57 |
| MsG0780036553.01 | MsG0780041232.01 | 0.833074 | 6.571250e-56 | 7.486398e-53 |
| MsG0780038647.01 | MsG0780041232.01 | 0.816241 | 6.121220e-52 | 4.314146e-49 |
| MsG0780038736.01 | MsG0780041232.01 | 0.822906 | 1.843702e-53 | 1.563860e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041232.01.T01 | MTR_7g107150 | 95.455 | 88 | 4 | 0 | 1 | 88 | 66 | 153 | 3.81e-46 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041232.01.T01 | AT5G06590 | 51.429 | 70 | 27 | 2 | 4 | 67 | 69 | 137 | 1.66e-13 | 64.3 |
| MsG0780041232.01.T01 | AT5G06590 | 51.429 | 70 | 27 | 2 | 4 | 67 | 69 | 137 | 1.72e-13 | 64.3 |
Find 21 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCTTCCTCCTCAATTTC+AGG | 0.165758 | 7:-88547796 | None:intergenic |
| GATAGTCCCTCTTCTTTCTA+TGG | 0.224713 | 7:+88547862 | MsG0780041232.01.T01:CDS |
| GCAGGTTTCGGATGATAAAT+TGG | 0.276771 | 7:+88547840 | MsG0780041232.01.T01:CDS |
| TCACTCTCAATGGCCGATTC+TGG | 0.318237 | 7:+88547886 | MsG0780041232.01.T01:CDS |
| AGCTGCTATGGCTACGTTCC+AGG | 0.330522 | 7:+88547674 | MsG0780041232.01.T01:CDS |
| TGTACAAGCGCCGCAGGTTT+CGG | 0.382807 | 7:+88547828 | MsG0780041232.01.T01:CDS |
| TGATTTAGTTCCTGAAATTG+AGG | 0.477536 | 7:+88547786 | MsG0780041232.01.T01:intron |
| GAACTTCTTGAAGCCAGAAT+CGG | 0.483419 | 7:-88547899 | None:intergenic |
| CTCTTCAAAGGAAACCGTGC+TGG | 0.493028 | 7:-88547579 | None:intergenic |
| TGAATCGAGCAGTTCGAGAT+CGG | 0.501516 | 7:-88547552 | None:intergenic |
| TTATGATGTACAAGCGCCGC+AGG | 0.539382 | 7:+88547822 | MsG0780041232.01.T01:CDS |
| TCTTGTACAATTCGTTGCAG+TGG | 0.542890 | 7:-88547610 | None:intergenic |
| GATCGTCTTAAAAGCTGCTA+TGG | 0.555122 | 7:+88547662 | MsG0780041232.01.T01:CDS |
| TTTCTATGGATCACTCTCAA+TGG | 0.556386 | 7:+88547876 | MsG0780041232.01.T01:CDS |
| GTGGCCTAAAAGCTCTTCAA+AGG | 0.578631 | 7:-88547591 | None:intergenic |
| AGTGATCCATAGAAAGAAGA+GGG | 0.586786 | 7:-88547868 | None:intergenic |
| ATCTTCGAGTTCGATTAGAT+CGG | 0.595046 | 7:-88547642 | None:intergenic |
| GAGTGATCCATAGAAAGAAG+AGG | 0.611635 | 7:-88547869 | None:intergenic |
| TTTAGTTCCTGAAATTGAGG+AGG | 0.651521 | 7:+88547789 | MsG0780041232.01.T01:intron |
| GCTCGATTCAATGTCCAGCA+CGG | 0.657391 | 7:+88547565 | MsG0780041232.01.T01:exon |
| AATTTATCATCCGAAACCTG+CGG | 0.707837 | 7:-88547838 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACACTAAAAACTAAACTTAA+CGG | - | Chr7:88547720-88547739 | None:intergenic | 20.0% |
| !! | TATGCATTTTTACAAATACC+TGG | - | Chr7:88547695-88547714 | None:intergenic | 25.0% |
| ! | TGATTTAGTTCCTGAAATTG+AGG | + | Chr7:88547786-88547805 | MsG0780041232.01.T01:intron | 30.0% |
| AATTTATCATCCGAAACCTG+CGG | - | Chr7:88547841-88547860 | None:intergenic | 35.0% | |
| AGTGATCCATAGAAAGAAGA+GGG | - | Chr7:88547871-88547890 | None:intergenic | 35.0% | |
| TTTCTATGGATCACTCTCAA+TGG | + | Chr7:88547876-88547895 | MsG0780041232.01.T01:CDS | 35.0% | |
| ! | ATCTTCGAGTTCGATTAGAT+CGG | - | Chr7:88547645-88547664 | None:intergenic | 35.0% |
| ! | TTTAGTTCCTGAAATTGAGG+AGG | + | Chr7:88547789-88547808 | MsG0780041232.01.T01:intron | 35.0% |
| !! | GTTTCCTTTGAAGAGCTTTT+AGG | + | Chr7:88547587-88547606 | MsG0780041232.01.T01:CDS | 35.0% |
| GAACTTCTTGAAGCCAGAAT+CGG | - | Chr7:88547902-88547921 | None:intergenic | 40.0% | |
| GAGTGATCCATAGAAAGAAG+AGG | - | Chr7:88547872-88547891 | None:intergenic | 40.0% | |
| GATAGTCCCTCTTCTTTCTA+TGG | + | Chr7:88547862-88547881 | MsG0780041232.01.T01:CDS | 40.0% | |
| GATCGTCTTAAAAGCTGCTA+TGG | + | Chr7:88547662-88547681 | MsG0780041232.01.T01:CDS | 40.0% | |
| GCAGGTTTCGGATGATAAAT+TGG | + | Chr7:88547840-88547859 | MsG0780041232.01.T01:CDS | 40.0% | |
| TCATCTTCCTCCTCAATTTC+AGG | - | Chr7:88547799-88547818 | None:intergenic | 40.0% | |
| TCTTGTACAATTCGTTGCAG+TGG | - | Chr7:88547613-88547632 | None:intergenic | 40.0% | |
| GTGGCCTAAAAGCTCTTCAA+AGG | - | Chr7:88547594-88547613 | None:intergenic | 45.0% | |
| TGAATCGAGCAGTTCGAGAT+CGG | - | Chr7:88547555-88547574 | None:intergenic | 45.0% | |
| CTCTTCAAAGGAAACCGTGC+TGG | - | Chr7:88547582-88547601 | None:intergenic | 50.0% | |
| GCTCGATTCAATGTCCAGCA+CGG | + | Chr7:88547565-88547584 | MsG0780041232.01.T01:exon | 50.0% | |
| ! | TCACTCTCAATGGCCGATTC+TGG | + | Chr7:88547886-88547905 | MsG0780041232.01.T01:CDS | 50.0% |
| ! | TTATGATGTACAAGCGCCGC+AGG | + | Chr7:88547822-88547841 | MsG0780041232.01.T01:CDS | 50.0% |
| AGCTGCTATGGCTACGTTCC+AGG | + | Chr7:88547674-88547693 | MsG0780041232.01.T01:CDS | 55.0% | |
| ! | TGTACAAGCGCCGCAGGTTT+CGG | + | Chr7:88547828-88547847 | MsG0780041232.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 88547539 | 88547945 | 88547539 | ID=MsG0780041232.01;Name=MsG0780041232.01 |
| Chr7 | mRNA | 88547539 | 88547945 | 88547539 | ID=MsG0780041232.01.T01;Parent=MsG0780041232.01;Name=MsG0780041232.01.T01;_AED=0.45;_eAED=0.46;_QI=36|1|0.5|1|1|1|2|0|90 |
| Chr7 | exon | 88547539 | 88547695 | 88547539 | ID=MsG0780041232.01.T01:exon:12143;Parent=MsG0780041232.01.T01 |
| Chr7 | exon | 88547794 | 88547945 | 88547794 | ID=MsG0780041232.01.T01:exon:12144;Parent=MsG0780041232.01.T01 |
| Chr7 | five_prime_UTR | 88547539 | 88547574 | 88547539 | ID=MsG0780041232.01.T01:five_prime_utr;Parent=MsG0780041232.01.T01 |
| Chr7 | CDS | 88547575 | 88547695 | 88547575 | ID=MsG0780041232.01.T01:cds;Parent=MsG0780041232.01.T01 |
| Chr7 | CDS | 88547794 | 88547945 | 88547794 | ID=MsG0780041232.01.T01:cds;Parent=MsG0780041232.01.T01 |
| Gene Sequence |
| Protein sequence |
