Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019935.01.T01 | XP_003595736.1 | 72.01 | 418 | 45 | 5 | 1 | 351 | 1 | 413 | 1.56E-178 | 511 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019935.01.T01 | O48716 | 37.963 | 324 | 129 | 9 | 66 | 319 | 97 | 418 | 2.18E-58 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019935.01.T01 | G7IGQ9 | 72.010 | 418 | 45 | 5 | 1 | 351 | 1 | 413 | 7.45e-179 | 511 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0480019935.01 | 0.820447 | 6.827306e-53 | 5.403481e-50 |
| MsG0180000076.01 | MsG0480019935.01 | 0.802519 | 5.398356e-49 | 2.649740e-46 |
| MsG0180000367.01 | MsG0480019935.01 | 0.801474 | 8.853343e-49 | 4.231893e-46 |
| MsG0180001137.01 | MsG0480019935.01 | -0.804424 | 2.174576e-49 | 1.120782e-46 |
| MsG0180003504.01 | MsG0480019935.01 | 0.815994 | 6.950896e-52 | 4.865843e-49 |
| MsG0180004510.01 | MsG0480019935.01 | 0.800978 | 1.118453e-48 | 5.279962e-46 |
| MsG0180004705.01 | MsG0480019935.01 | 0.841865 | 3.666847e-58 | 5.460312e-55 |
| MsG0180005369.01 | MsG0480019935.01 | -0.835388 | 1.727104e-56 | 2.108924e-53 |
| MsG0180006182.01 | MsG0480019935.01 | 0.839174 | 1.855444e-57 | 2.541479e-54 |
| MsG0180006191.01 | MsG0480019935.01 | 0.805762 | 1.141669e-49 | 6.090854e-47 |
| MsG0280006381.01 | MsG0480019935.01 | -0.804223 | 2.395330e-49 | 1.228167e-46 |
| MsG0380013041.01 | MsG0480019935.01 | 0.833570 | 4.943544e-56 | 5.715437e-53 |
| MsG0380013928.01 | MsG0480019935.01 | -0.800811 | 1.209557e-48 | 5.685382e-46 |
| MsG0380014029.01 | MsG0480019935.01 | -0.801160 | 1.026555e-48 | 4.868317e-46 |
| MsG0380014642.01 | MsG0480019935.01 | -0.803846 | 2.868687e-49 | 1.456851e-46 |
| MsG0480018563.01 | MsG0480019935.01 | 0.814379 | 1.587277e-51 | 1.063393e-48 |
| MsG0480019545.01 | MsG0480019935.01 | 0.800137 | 1.659387e-48 | 7.667845e-46 |
| MsG0480019935.01 | MsG0480020722.01 | 0.809025 | 2.319977e-50 | 1.347144e-47 |
| MsG0480019935.01 | MsG0480021238.01 | 0.813607 | 2.349044e-51 | 1.541121e-48 |
| MsG0480019935.01 | MsG0480021433.01 | 0.815257 | 1.014100e-51 | 6.956822e-49 |
| MsG0480019935.01 | MsG0480022242.01 | -0.809568 | 1.774226e-50 | 1.045346e-47 |
| MsG0480019935.01 | MsG0480022833.01 | 0.811635 | 6.343082e-51 | 3.946618e-48 |
| MsG0480019935.01 | MsG0480023322.01 | 0.849866 | 2.455596e-60 | 4.720795e-57 |
| MsG0480019935.01 | MsG0580024707.01 | 0.807789 | 4.256602e-50 | 2.392666e-47 |
| MsG0480019935.01 | MsG0580025431.01 | 0.811413 | 7.089208e-51 | 4.385442e-48 |
| MsG0480019935.01 | MsG0580025464.01 | 0.817122 | 3.883376e-52 | 2.803668e-49 |
| MsG0480019935.01 | MsG0580025486.01 | -0.814844 | 1.252284e-51 | 8.495296e-49 |
| MsG0480019935.01 | MsG0580025633.01 | 0.801722 | 7.872979e-49 | 3.786811e-46 |
| MsG0480019935.01 | MsG0580026311.01 | 0.816189 | 6.287420e-52 | 4.425038e-49 |
| MsG0480019935.01 | MsG0580028302.01 | 0.823447 | 1.378405e-53 | 1.187118e-50 |
| MsG0480019935.01 | MsG0580029438.01 | 0.826035 | 3.382361e-54 | 3.135911e-51 |
| MsG0480019935.01 | MsG0580029696.01 | 0.808916 | 2.447752e-50 | 1.417188e-47 |
| MsG0480019935.01 | MsG0580029751.01 | -0.816978 | 4.184966e-52 | 3.009771e-49 |
| MsG0480019935.01 | MsG0580030179.01 | 0.829951 | 3.861780e-55 | 4.011816e-52 |
| MsG0480019935.01 | MsG0680030635.01 | -0.835963 | 1.235685e-56 | 1.534968e-53 |
| MsG0480019935.01 | MsG0680034301.01 | 0.812838 | 3.466131e-51 | 2.227009e-48 |
| MsG0480019935.01 | MsG0680034362.01 | 0.810005 | 1.428885e-50 | 8.516357e-48 |
| MsG0480019935.01 | MsG0680034415.01 | 0.823235 | 1.544966e-53 | 1.322649e-50 |
| MsG0480019935.01 | MsG0680035584.01 | -0.818939 | 1.508927e-52 | 1.145375e-49 |
| MsG0480019935.01 | MsG0780036611.01 | 0.803300 | 3.722810e-49 | 1.864113e-46 |
| MsG0480019935.01 | MsG0780038307.01 | 0.812733 | 3.653916e-51 | 2.341390e-48 |
| MsG0480019935.01 | MsG0780038810.01 | 0.846915 | 1.608409e-59 | 2.811263e-56 |
| MsG0480019935.01 | MsG0780039821.01 | -0.807916 | 4.000116e-50 | 2.255831e-47 |
| MsG0480019935.01 | MsG0780039916.01 | 0.835711 | 1.431526e-56 | 1.765005e-53 |
| MsG0480019935.01 | MsG0780041100.01 | 0.801404 | 9.147665e-49 | 4.364845e-46 |
| MsG0480019935.01 | MsG0780041180.01 | 0.816128 | 6.486905e-52 | 4.557672e-49 |
| MsG0480019935.01 | MsG0880042122.01 | 0.818799 | 1.623567e-52 | 1.227420e-49 |
| MsG0480019935.01 | MsG0880042633.01 | 0.813034 | 3.139851e-51 | 2.028061e-48 |
| MsG0480019935.01 | MsG0880042635.01 | 0.853581 | 2.173412e-61 | 4.719038e-58 |
| MsG0480019935.01 | MsG0880043550.01 | 0.810708 | 1.008006e-50 | 6.120258e-48 |
| MsG0480019935.01 | MsG0880044688.01 | 0.831672 | 1.461854e-55 | 1.597485e-52 |
| MsG0480019935.01 | MsG0880045503.01 | 0.807915 | 4.002587e-50 | 2.257139e-47 |
| MsG0480019935.01 | MsG0880045703.01 | -0.823758 | 1.165838e-53 | 1.013053e-50 |
| MsG0480019935.01 | MsG0880045852.01 | 0.832277 | 1.036243e-55 | 1.152578e-52 |
| MsG0480019935.01 | MsG0880045856.01 | 0.800497 | 1.401857e-48 | 6.537183e-46 |
| MsG0480019935.01 | MsG0880046010.01 | -0.805647 | 1.206474e-49 | 6.417387e-47 |
| MsG0480019935.01 | MsG0880046945.01 | 0.810678 | 1.022693e-50 | 6.204542e-48 |
| MsG0480019935.01 | MsG0880047057.01 | 0.836938 | 6.976687e-57 | 8.925710e-54 |
| MsG0480019935.01 | MsG0880047714.01 | 0.813475 | 2.512701e-51 | 1.642582e-48 |
| MsG0380016110.01 | MsG0480019935.01 | 0.838523 | 2.734607e-57 | 3.672097e-54 |
| MsG0380016485.01 | MsG0480019935.01 | 0.803508 | 3.370931e-49 | 1.697098e-46 |
| MsG0380016934.01 | MsG0480019935.01 | -0.800131 | 1.664293e-48 | 7.689286e-46 |
| MsG0380016935.01 | MsG0480019935.01 | -0.811246 | 7.704320e-51 | 4.744921e-48 |
| MsG0380017030.01 | MsG0480019935.01 | 0.801332 | 9.463717e-49 | 4.507507e-46 |
| MsG0380017898.01 | MsG0480019935.01 | 0.812043 | 5.169127e-51 | 3.251512e-48 |
| MsG0380017997.01 | MsG0480019935.01 | 0.817484 | 3.220626e-52 | 2.348443e-49 |
| MsG0380018004.01 | MsG0480019935.01 | 0.817997 | 2.466474e-52 | 1.823976e-49 |
| MsG0280007281.01 | MsG0480019935.01 | 0.823943 | 1.054854e-53 | 9.213750e-51 |
| MsG0280007667.01 | MsG0480019935.01 | 0.800688 | 1.281680e-48 | 6.005681e-46 |
| MsG0280007786.01 | MsG0480019935.01 | 0.848543 | 5.731911e-60 | 1.055479e-56 |
| MsG0280009167.01 | MsG0480019935.01 | 0.803867 | 2.840304e-49 | 1.443215e-46 |
| MsG0280009526.01 | MsG0480019935.01 | 0.818646 | 1.758338e-52 | 1.323708e-49 |
| MsG0280009742.01 | MsG0480019935.01 | -0.812898 | 3.363234e-51 | 2.164424e-48 |
| MsG0280010142.01 | MsG0480019935.01 | -0.801937 | 7.113751e-49 | 3.440394e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480019935.01 | MsG0680035806.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019935.01.T01 | MTR_2g060070 | 72.010 | 418 | 45 | 5 | 1 | 351 | 1 | 413 | 0.0 | 511 |
| MsG0480019935.01.T01 | MTR_1g021590 | 35.447 | 347 | 145 | 9 | 42 | 317 | 91 | 429 | 1.55e-55 | 188 |
| MsG0480019935.01.T01 | MTR_8g089650 | 39.249 | 293 | 144 | 8 | 52 | 317 | 83 | 368 | 3.31e-55 | 187 |
| MsG0480019935.01.T01 | MTR_5g018520 | 39.568 | 278 | 131 | 8 | 66 | 317 | 98 | 364 | 5.48e-55 | 187 |
| MsG0480019935.01.T01 | MTR_4g068457 | 37.544 | 285 | 141 | 6 | 61 | 317 | 124 | 399 | 6.48e-54 | 185 |
| MsG0480019935.01.T01 | MTR_3g078380 | 34.954 | 329 | 160 | 13 | 34 | 317 | 33 | 352 | 5.03e-45 | 159 |
| MsG0480019935.01.T01 | MTR_5g035980 | 32.203 | 354 | 156 | 13 | 51 | 336 | 57 | 394 | 1.17e-44 | 159 |
| MsG0480019935.01.T01 | MTR_4g104410 | 33.987 | 306 | 163 | 9 | 41 | 317 | 13 | 308 | 1.00e-40 | 147 |
| MsG0480019935.01.T01 | MTR_4g106480 | 32.867 | 286 | 149 | 8 | 68 | 317 | 60 | 338 | 3.84e-40 | 146 |
| MsG0480019935.01.T01 | MTR_3g110185 | 28.488 | 344 | 165 | 11 | 55 | 324 | 78 | 414 | 3.99e-35 | 136 |
| MsG0480019935.01.T01 | MTR_2g101410 | 29.329 | 283 | 151 | 12 | 66 | 317 | 85 | 349 | 2.89e-22 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019935.01.T01 | AT3G51930 | 70.916 | 251 | 52 | 4 | 23 | 261 | 28 | 269 | 6.04e-117 | 345 |
| MsG0480019935.01.T01 | AT1G24530 | 41.472 | 299 | 137 | 9 | 47 | 317 | 63 | 351 | 1.33e-60 | 200 |
| MsG0480019935.01.T01 | AT2G26490 | 37.963 | 324 | 129 | 9 | 66 | 319 | 97 | 418 | 2.22e-59 | 198 |
| MsG0480019935.01.T01 | AT1G49450 | 33.249 | 397 | 170 | 9 | 13 | 322 | 81 | 469 | 8.84e-56 | 189 |
| MsG0480019935.01.T01 | AT3G18950 | 36.364 | 330 | 134 | 7 | 66 | 321 | 139 | 466 | 3.02e-53 | 182 |
| MsG0480019935.01.T01 | AT1G47610 | 34.954 | 329 | 140 | 9 | 66 | 322 | 25 | 351 | 4.91e-53 | 179 |
| MsG0480019935.01.T01 | AT1G24130 | 37.255 | 306 | 144 | 10 | 52 | 317 | 56 | 353 | 3.84e-51 | 176 |
| MsG0480019935.01.T01 | AT3G50390 | 35.495 | 293 | 149 | 7 | 58 | 321 | 91 | 372 | 1.43e-49 | 172 |
| MsG0480019935.01.T01 | AT4G34380 | 33.634 | 333 | 140 | 8 | 66 | 319 | 126 | 456 | 5.82e-47 | 166 |
| MsG0480019935.01.T01 | AT5G50120 | 34.084 | 311 | 150 | 9 | 55 | 317 | 27 | 330 | 4.42e-45 | 159 |
| MsG0480019935.01.T01 | AT5G50120 | 25.830 | 271 | 136 | 10 | 64 | 319 | 164 | 384 | 3.79e-13 | 70.5 |
| MsG0480019935.01.T01 | AT5G40880 | 26.068 | 234 | 115 | 5 | 106 | 317 | 238 | 435 | 9.86e-14 | 72.4 |
| MsG0480019935.01.T01 | AT5G40880 | 26.068 | 234 | 115 | 5 | 106 | 317 | 267 | 464 | 1.16e-13 | 72.4 |
| MsG0480019935.01.T01 | AT4G02730 | 25.784 | 287 | 155 | 11 | 55 | 317 | 33 | 285 | 6.22e-13 | 69.3 |
| MsG0480019935.01.T01 | AT5G49200 | 22.881 | 236 | 121 | 5 | 106 | 317 | 213 | 411 | 5.34e-11 | 63.9 |
Find 69 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAATGGTTTGATTTATTC+AGG | 0.146599 | 4:-30037039 | MsG0480019935.01.T01:CDS |
| TCCAACAACAAAAGACTATT+TGG | 0.161852 | 4:-30037157 | MsG0480019935.01.T01:CDS |
| TGTAAAGTTGGGGTTATAAT+TGG | 0.191734 | 4:-30036581 | MsG0480019935.01.T01:CDS |
| TATGGCAACAACCAGATTTA+AGG | 0.199159 | 4:-30037329 | MsG0480019935.01.T01:CDS |
| CAGATAAAAGTATAGGTATT+TGG | 0.236613 | 4:-30036628 | MsG0480019935.01.T01:CDS |
| GTCAAAGCACTAGCAACTTT+TGG | 0.264942 | 4:-30037273 | MsG0480019935.01.T01:CDS |
| TCTTGTTTGGTTGTTCATAA+TGG | 0.291146 | 4:-30037054 | MsG0480019935.01.T01:CDS |
| TTGGTAAAATTTGTAAAGTT+GGG | 0.317130 | 4:-30036592 | MsG0480019935.01.T01:CDS |
| GGGATAAGACACTTAAAGTT+TGG | 0.330304 | 4:-30037011 | MsG0480019935.01.T01:CDS |
| TTCGCAAACAACCTTAAATC+TGG | 0.338735 | 4:+30037318 | None:intergenic |
| CCAACAACAAAAGACTATTT+GGG | 0.358717 | 4:-30037156 | MsG0480019935.01.T01:CDS |
| TTTGGTAAAATTTGTAAAGT+TGG | 0.360311 | 4:-30036593 | MsG0480019935.01.T01:CDS |
| TTCGTGTTTGGAAAGTTTCC+AGG | 0.367861 | 4:-30037215 | MsG0480019935.01.T01:CDS |
| TGCTGATAGCATTTCTTGTT+TGG | 0.374831 | 4:-30037067 | MsG0480019935.01.T01:CDS |
| TTAAGGTTGTTTGCGAAATT+CGG | 0.381571 | 4:-30037312 | MsG0480019935.01.T01:CDS |
| TGATGATGGAGTAGAAAGTA+TGG | 0.391324 | 4:+30037572 | None:intergenic |
| GAAGTGTTTAGAATCTATTA+AGG | 0.398543 | 4:-30036974 | MsG0480019935.01.T01:CDS |
| TTGATAGAAGTGTGAGAGTT+TGG | 0.399809 | 4:-30036478 | MsG0480019935.01.T01:CDS |
| ATCAGTTTCTCATAGATCAT+TGG | 0.403558 | 4:-30037442 | MsG0480019935.01.T01:CDS |
| TTCTGCAGATGGGAAAATTA+AGG | 0.406190 | 4:-30036896 | MsG0480019935.01.T01:CDS |
| TTTGAAGCAAGGGTATTCAT+TGG | 0.409211 | 4:+30037597 | None:intergenic |
| GCTTCATCATCTAATAGAAT+TGG | 0.416552 | 4:-30036530 | MsG0480019935.01.T01:CDS |
| GCGAAATTCGGTCAAGGCGA+TGG | 0.421578 | 4:-30037300 | MsG0480019935.01.T01:CDS |
| AAGATAGCAAGATTCGTGTT+TGG | 0.431533 | 4:-30037227 | MsG0480019935.01.T01:CDS |
| GTTTGATTTATTCAGGGTCA+TGG | 0.443041 | 4:-30037032 | MsG0480019935.01.T01:CDS |
| CAATATGAACTCACCACATA+AGG | 0.445391 | 4:+30037380 | None:intergenic |
| GAACACATTCTCTGAACTCC+TGG | 0.454182 | 4:+30037197 | None:intergenic |
| AGGGCAAAGACATCATAGTA+TGG | 0.455652 | 4:-30037347 | MsG0480019935.01.T01:CDS |
| GTTTATTCTGCTTCTGCAGA+TGG | 0.457417 | 4:-30036907 | MsG0480019935.01.T01:CDS |
| ATTTGGAAAAGAGAGCCTTT+TGG | 0.468370 | 4:-30036611 | MsG0480019935.01.T01:CDS |
| TTGCTTCTACAGCTTGATGA+TGG | 0.471064 | 4:+30037558 | None:intergenic |
| ATGGGTTGGTAGCATGTAAA+GGG | 0.476916 | 4:-30036933 | MsG0480019935.01.T01:CDS |
| GTTTCATGTTTAGCCTTATG+TGG | 0.477781 | 4:-30037393 | MsG0480019935.01.T01:CDS |
| GCACATGATGATGCTATCAA+TGG | 0.482687 | 4:-30036952 | MsG0480019935.01.T01:CDS |
| GTTCATATTGAGTGCATCAC+AGG | 0.491312 | 4:-30037367 | MsG0480019935.01.T01:CDS |
| AATGGGTTGGTAGCATGTAA+AGG | 0.503199 | 4:-30036934 | MsG0480019935.01.T01:CDS |
| TGGTAAAATTTGTAAAGTTG+GGG | 0.503634 | 4:-30036591 | MsG0480019935.01.T01:CDS |
| GGATCAGCAGATAAAAGTAT+AGG | 0.505453 | 4:-30036635 | MsG0480019935.01.T01:CDS |
| GATTTGCATGGTGGTGGTGA+TGG | 0.512681 | 4:+30037462 | None:intergenic |
| ATAATGGTTTGATTTATTCA+GGG | 0.512775 | 4:-30037038 | MsG0480019935.01.T01:CDS |
| ATTAAGGCATGGGGAAAACA+AGG | 0.514895 | 4:-30036880 | MsG0480019935.01.T01:CDS |
| TTTGATTTATTCAGGGTCAT+GGG | 0.520892 | 4:-30037031 | MsG0480019935.01.T01:CDS |
| TTTATTCTGCTTCTGCAGAT+GGG | 0.532550 | 4:-30036906 | MsG0480019935.01.T01:CDS |
| GGAGACATCACAAGAGGTTG+TGG | 0.537342 | 4:-30037098 | MsG0480019935.01.T01:CDS |
| AAAGTATGATGAAACAAAAG+TGG | 0.538411 | 4:-30036447 | MsG0480019935.01.T01:CDS |
| AAACTCTCACACTTCTATCA+AGG | 0.539419 | 4:+30036480 | None:intergenic |
| GGGGTTATAATTGGACATGA+AGG | 0.541474 | 4:-30036572 | MsG0480019935.01.T01:CDS |
| AGATGGGAAAATTAAGGCAT+GGG | 0.554116 | 4:-30036890 | MsG0480019935.01.T01:CDS |
| TCATCATCTAATAGAATTGG+TGG | 0.559788 | 4:-30036527 | MsG0480019935.01.T01:CDS |
| TAGAAGTGTGAGAGTTTGGT+GGG | 0.560073 | 4:-30036474 | MsG0480019935.01.T01:CDS |
| TCATCTAATAGAATTGGTGG+TGG | 0.562304 | 4:-30036524 | MsG0480019935.01.T01:CDS |
| CAGATGGGAAAATTAAGGCA+TGG | 0.562592 | 4:-30036891 | MsG0480019935.01.T01:CDS |
| AAAGTAATTATGTTCAAACT+AGG | 0.570479 | 4:-30037119 | MsG0480019935.01.T01:CDS |
| ATAGAAGTGTGAGAGTTTGG+TGG | 0.573934 | 4:-30036475 | MsG0480019935.01.T01:CDS |
| GAAGCTTGTAAACACTTCAC+AGG | 0.574638 | 4:+30036548 | None:intergenic |
| TTGTTTGCGAAATTCGGTCA+AGG | 0.581090 | 4:-30037306 | MsG0480019935.01.T01:CDS |
| TCATCAAGCTGTAGAAGCAA+TGG | 0.583309 | 4:-30037555 | MsG0480019935.01.T01:CDS |
| CACATGATGATGCTATCAAT+GGG | 0.607884 | 4:-30036951 | MsG0480019935.01.T01:CDS |
| TTCATATTGAGTGCATCACA+GGG | 0.620924 | 4:-30037366 | MsG0480019935.01.T01:CDS |
| TGATGATGCTATCAATGGGT+TGG | 0.627455 | 4:-30036947 | MsG0480019935.01.T01:CDS |
| CAACAACAAAAGACTATTTG+GGG | 0.631861 | 4:-30037155 | MsG0480019935.01.T01:CDS |
| GAAACTGATTTGCATGGTGG+TGG | 0.634072 | 4:+30037456 | None:intergenic |
| GTATGATGAAACAAAAGTGG+AGG | 0.645051 | 4:-30036444 | MsG0480019935.01.T01:CDS |
| CTATGAGAAACTGATTTGCA+TGG | 0.653319 | 4:+30037450 | None:intergenic |
| GATGGGAAAATTAAGGCATG+GGG | 0.658308 | 4:-30036889 | MsG0480019935.01.T01:CDS |
| GCGATTCTTTCAGAACACGT+TGG | 0.661081 | 4:-30037420 | MsG0480019935.01.T01:CDS |
| AAACTAGGAGACATCACAAG+AGG | 0.664661 | 4:-30037104 | MsG0480019935.01.T01:CDS |
| TTAAGGCATGGGGAAAACAA+GGG | 0.674587 | 4:-30036879 | MsG0480019935.01.T01:CDS |
| TGAGAAACTGATTTGCATGG+TGG | 0.728963 | 4:+30037453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTTGCATACTTTGAA+AGG | - | Chr4:30037111-30037130 | MsG0480019935.01.T01:CDS | 20.0% |
| !! | AAAGTAATTATGTTCAAACT+AGG | - | Chr4:30036848-30036867 | MsG0480019935.01.T01:CDS | 20.0% |
| !! | TTGGTAAAATTTGTAAAGTT+GGG | - | Chr4:30037375-30037394 | MsG0480019935.01.T01:CDS | 20.0% |
| !! | TTTGGTAAAATTTGTAAAGT+TGG | - | Chr4:30037374-30037393 | MsG0480019935.01.T01:CDS | 20.0% |
| !!! | ACTTTACAAATTTTACCAAA+AGG | + | Chr4:30037374-30037393 | None:intergenic | 20.0% |
| !!! | ATAATGGTTTGATTTATTCA+GGG | - | Chr4:30036929-30036948 | MsG0480019935.01.T01:CDS | 20.0% |
| !!! | ATGAAAGAAATTTTGAGAAT+TGG | - | Chr4:30037236-30037255 | MsG0480019935.01.T01:CDS | 20.0% |
| ! | AAAGTATGATGAAACAAAAG+TGG | - | Chr4:30037520-30037539 | MsG0480019935.01.T01:CDS | 25.0% |
| ! | TGGTAAAATTTGTAAAGTTG+GGG | - | Chr4:30037376-30037395 | MsG0480019935.01.T01:CDS | 25.0% |
| !! | CAGATAAAAGTATAGGTATT+TGG | - | Chr4:30037339-30037358 | MsG0480019935.01.T01:CDS | 25.0% |
| !! | CATAATGGTTTGATTTATTC+AGG | - | Chr4:30036928-30036947 | MsG0480019935.01.T01:CDS | 25.0% |
| !! | GAAGTGTTTAGAATCTATTA+AGG | - | Chr4:30036993-30037012 | MsG0480019935.01.T01:CDS | 25.0% |
| !!! | ACTTTTGTTTCATCATACTT+TGG | + | Chr4:30037521-30037540 | None:intergenic | 25.0% |
| !!! | GAAATTTTGAGAATTGGAAA+TGG | - | Chr4:30037242-30037261 | MsG0480019935.01.T01:CDS | 25.0% |
| ATCAGTTTCTCATAGATCAT+TGG | - | Chr4:30036525-30036544 | MsG0480019935.01.T01:CDS | 30.0% | |
| CAACAACAAAAGACTATTTG+GGG | - | Chr4:30036812-30036831 | MsG0480019935.01.T01:intron | 30.0% | |
| CCAACAACAAAAGACTATTT+GGG | - | Chr4:30036811-30036830 | MsG0480019935.01.T01:intron | 30.0% | |
| GCTTCATCATCTAATAGAAT+TGG | - | Chr4:30037437-30037456 | MsG0480019935.01.T01:CDS | 30.0% | |
| GTTTGTGTATGTGTTTGATT+GGG | - | Chr4:30037294-30037313 | MsG0480019935.01.T01:CDS | 30.0% | |
| TCATCATCTAATAGAATTGG+TGG | - | Chr4:30037440-30037459 | MsG0480019935.01.T01:CDS | 30.0% | |
| TCCAACAACAAAAGACTATT+TGG | - | Chr4:30036810-30036829 | MsG0480019935.01.T01:intron | 30.0% | |
| TGTAAAGTTGGGGTTATAAT+TGG | - | Chr4:30037386-30037405 | MsG0480019935.01.T01:CDS | 30.0% | |
| TGTTTGTGTATGTGTTTGAT+TGG | - | Chr4:30037293-30037312 | MsG0480019935.01.T01:CDS | 30.0% | |
| TTAAGGTTGTTTGCGAAATT+CGG | - | Chr4:30036655-30036674 | MsG0480019935.01.T01:intron | 30.0% | |
| TTTGTGTATGTGTTTGATTG+GGG | - | Chr4:30037295-30037314 | MsG0480019935.01.T01:CDS | 30.0% | |
| ! | TCTTGTTTGGTTGTTCATAA+TGG | - | Chr4:30036913-30036932 | MsG0480019935.01.T01:CDS | 30.0% |
| ! | TTTGATTTATTCAGGGTCAT+GGG | - | Chr4:30036936-30036955 | MsG0480019935.01.T01:CDS | 30.0% |
| !! | GTGGATTTTTGTTGTATAGT+GGG | - | Chr4:30037462-30037481 | MsG0480019935.01.T01:CDS | 30.0% |
| !!! | AAAGTATGGATTTTGAAGCA+AGG | + | Chr4:30036384-30036403 | None:intergenic | 30.0% |
| !!! | AAGTATGGATTTTGAAGCAA+GGG | + | Chr4:30036383-30036402 | None:intergenic | 30.0% |
| !!! | ACTTTGAAAGGTGTTTTAGA+GGG | - | Chr4:30037123-30037142 | MsG0480019935.01.T01:CDS | 30.0% |
| !!! | TACTTTGAAAGGTGTTTTAG+AGG | - | Chr4:30037122-30037141 | MsG0480019935.01.T01:CDS | 30.0% |
| !!! | TGTTTTCTGATGATGGAAAT+GGG | - | Chr4:30037176-30037195 | MsG0480019935.01.T01:CDS | 30.0% |
| !!! | TTGTTTTCTGATGATGGAAA+TGG | - | Chr4:30037175-30037194 | MsG0480019935.01.T01:CDS | 30.0% |
| AAACTCTCACACTTCTATCA+AGG | + | Chr4:30037490-30037509 | None:intergenic | 35.0% | |
| AAGATAGCAAGATTCGTGTT+TGG | - | Chr4:30036740-30036759 | MsG0480019935.01.T01:intron | 35.0% | |
| AGATGGGAAAATTAAGGCAT+GGG | - | Chr4:30037077-30037096 | MsG0480019935.01.T01:CDS | 35.0% | |
| CAATATGAACTCACCACATA+AGG | + | Chr4:30036590-30036609 | None:intergenic | 35.0% | |
| GGGATAAGACACTTAAAGTT+TGG | - | Chr4:30036956-30036975 | MsG0480019935.01.T01:CDS | 35.0% | |
| GTATGATGAAACAAAAGTGG+AGG | - | Chr4:30037523-30037542 | MsG0480019935.01.T01:CDS | 35.0% | |
| TATGGCAACAACCAGATTTA+AGG | - | Chr4:30036638-30036657 | MsG0480019935.01.T01:intron | 35.0% | |
| TCATCTAATAGAATTGGTGG+TGG | - | Chr4:30037443-30037462 | MsG0480019935.01.T01:CDS | 35.0% | |
| TGCTGATAGCATTTCTTGTT+TGG | - | Chr4:30036900-30036919 | MsG0480019935.01.T01:CDS | 35.0% | |
| TTCATATTGAGTGCATCACA+GGG | - | Chr4:30036601-30036620 | MsG0480019935.01.T01:CDS | 35.0% | |
| TTCGCAAACAACCTTAAATC+TGG | + | Chr4:30036652-30036671 | None:intergenic | 35.0% | |
| TTCTGCAGATGGGAAAATTA+AGG | - | Chr4:30037071-30037090 | MsG0480019935.01.T01:CDS | 35.0% | |
| TTGTGTATGTGTTTGATTGG+GGG | - | Chr4:30037296-30037315 | MsG0480019935.01.T01:CDS | 35.0% | |
| TTTATTCTGCTTCTGCAGAT+GGG | - | Chr4:30037061-30037080 | MsG0480019935.01.T01:CDS | 35.0% | |
| ! | ATTTGGAAAAGAGAGCCTTT+TGG | - | Chr4:30037356-30037375 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | CACATGATGATGCTATCAAT+GGG | - | Chr4:30037016-30037035 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | GGAAATGGTTTGTGAAACAA+AGG | - | Chr4:30037257-30037276 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | GGATCAGCAGATAAAAGTAT+AGG | - | Chr4:30037332-30037351 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | GTTTGATTTATTCAGGGTCA+TGG | - | Chr4:30036935-30036954 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | TGAAACAAAGGCACATGAAA+TGG | - | Chr4:30037269-30037288 | MsG0480019935.01.T01:CDS | 35.0% |
| ! | TTGATAGAAGTGTGAGAGTT+TGG | - | Chr4:30037489-30037508 | MsG0480019935.01.T01:CDS | 35.0% |
| !! | CCCAAATAGTCTTTTGTTGT+TGG | + | Chr4:30036814-30036833 | None:intergenic | 35.0% |
| !! | CTATGAGAAACTGATTTGCA+TGG | + | Chr4:30036520-30036539 | None:intergenic | 35.0% |
| !! | GGTGGATTTTTGTTGTATAG+TGG | - | Chr4:30037461-30037480 | MsG0480019935.01.T01:CDS | 35.0% |
| !! | GTTTCATGTTTAGCCTTATG+TGG | - | Chr4:30036574-30036593 | MsG0480019935.01.T01:CDS | 35.0% |
| !! | TGATGATGGAGTAGAAAGTA+TGG | + | Chr4:30036398-30036417 | None:intergenic | 35.0% |
| !!! | CTGTTGTTGTTTTCTGATGA+TGG | - | Chr4:30037169-30037188 | MsG0480019935.01.T01:CDS | 35.0% |
| !!! | GATGATGGAAATGGGTTTTT+AGG | - | Chr4:30037184-30037203 | MsG0480019935.01.T01:CDS | 35.0% |
| AAACTAGGAGACATCACAAG+AGG | - | Chr4:30036863-30036882 | MsG0480019935.01.T01:CDS | 40.0% | |
| AGGGCAAAGACATCATAGTA+TGG | - | Chr4:30036620-30036639 | MsG0480019935.01.T01:CDS | 40.0% | |
| ATTAAGGCATGGGGAAAACA+AGG | - | Chr4:30037087-30037106 | MsG0480019935.01.T01:CDS | 40.0% | |
| CAGATGGGAAAATTAAGGCA+TGG | - | Chr4:30037076-30037095 | MsG0480019935.01.T01:CDS | 40.0% | |
| GAAGCTTGTAAACACTTCAC+AGG | + | Chr4:30037422-30037441 | None:intergenic | 40.0% | |
| GATGGGAAAATTAAGGCATG+GGG | - | Chr4:30037078-30037097 | MsG0480019935.01.T01:CDS | 40.0% | |
| GGGGTTATAATTGGACATGA+AGG | - | Chr4:30037395-30037414 | MsG0480019935.01.T01:CDS | 40.0% | |
| GTTCATATTGAGTGCATCAC+AGG | - | Chr4:30036600-30036619 | MsG0480019935.01.T01:CDS | 40.0% | |
| GTTTATTCTGCTTCTGCAGA+TGG | - | Chr4:30037060-30037079 | MsG0480019935.01.T01:CDS | 40.0% | |
| TCATCAAGCTGTAGAAGCAA+TGG | - | Chr4:30036412-30036431 | MsG0480019935.01.T01:CDS | 40.0% | |
| TTAAGGCATGGGGAAAACAA+GGG | - | Chr4:30037088-30037107 | MsG0480019935.01.T01:CDS | 40.0% | |
| TTCGTGTTTGGAAAGTTTCC+AGG | - | Chr4:30036752-30036771 | MsG0480019935.01.T01:intron | 40.0% | |
| TTGCTTCTACAGCTTGATGA+TGG | + | Chr4:30036412-30036431 | None:intergenic | 40.0% | |
| TTGTTTGCGAAATTCGGTCA+AGG | - | Chr4:30036661-30036680 | MsG0480019935.01.T01:intron | 40.0% | |
| ! | ATAGAAGTGTGAGAGTTTGG+TGG | - | Chr4:30037492-30037511 | MsG0480019935.01.T01:CDS | 40.0% |
| ! | GCACATGATGATGCTATCAA+TGG | - | Chr4:30037015-30037034 | MsG0480019935.01.T01:CDS | 40.0% |
| ! | TAGAAGTGTGAGAGTTTGGT+GGG | - | Chr4:30037493-30037512 | MsG0480019935.01.T01:CDS | 40.0% |
| ! | TGATGATGCTATCAATGGGT+TGG | - | Chr4:30037020-30037039 | MsG0480019935.01.T01:CDS | 40.0% |
| !! | AATGGGTTGGTAGCATGTAA+AGG | - | Chr4:30037033-30037052 | MsG0480019935.01.T01:CDS | 40.0% |
| !! | ATGGGTTGGTAGCATGTAAA+GGG | - | Chr4:30037034-30037053 | MsG0480019935.01.T01:CDS | 40.0% |
| !! | GATGGAAATGGGTTTTTAGG+AGG | - | Chr4:30037187-30037206 | MsG0480019935.01.T01:CDS | 40.0% |
| !! | GTCAAAGCACTAGCAACTTT+TGG | - | Chr4:30036694-30036713 | MsG0480019935.01.T01:intron | 40.0% |
| !! | TGAGAAACTGATTTGCATGG+TGG | + | Chr4:30036517-30036536 | None:intergenic | 40.0% |
| !!! | TTTTTAGGAGGTGGTTCTGA+TGG | - | Chr4:30037199-30037218 | MsG0480019935.01.T01:CDS | 40.0% |
| GAACACATTCTCTGAACTCC+TGG | + | Chr4:30036773-30036792 | None:intergenic | 45.0% | |
| GCGATTCTTTCAGAACACGT+TGG | - | Chr4:30036547-30036566 | MsG0480019935.01.T01:CDS | 45.0% | |
| !! | GAAACTGATTTGCATGGTGG+TGG | + | Chr4:30036514-30036533 | None:intergenic | 45.0% |
| !! | GGAAATGGGTTTTTAGGAGG+TGG | - | Chr4:30037190-30037209 | MsG0480019935.01.T01:CDS | 45.0% |
| !!! | ATTGGGGGTTTTTTGTGCAC+AGG | - | Chr4:30037311-30037330 | MsG0480019935.01.T01:CDS | 45.0% |
| GGAGACATCACAAGAGGTTG+TGG | - | Chr4:30036869-30036888 | MsG0480019935.01.T01:CDS | 50.0% | |
| !! | GATTTGCATGGTGGTGGTGA+TGG | + | Chr4:30036508-30036527 | None:intergenic | 50.0% |
| GCGAAATTCGGTCAAGGCGA+TGG | - | Chr4:30036667-30036686 | MsG0480019935.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 30036373 | 30037616 | 30036373 | ID=MsG0480019935.01;Name=MsG0480019935.01 |
| Chr4 | mRNA | 30036373 | 30037616 | 30036373 | ID=MsG0480019935.01.T01;Parent=MsG0480019935.01;Name=MsG0480019935.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|351 |
| Chr4 | exon | 30036845 | 30037616 | 30036845 | ID=MsG0480019935.01.T01:exon:9832;Parent=MsG0480019935.01.T01 |
| Chr4 | exon | 30036373 | 30036656 | 30036373 | ID=MsG0480019935.01.T01:exon:9831;Parent=MsG0480019935.01.T01 |
| Chr4 | CDS | 30036845 | 30037616 | 30036845 | ID=MsG0480019935.01.T01:cds;Parent=MsG0480019935.01.T01 |
| Chr4 | CDS | 30036373 | 30036656 | 30036373 | ID=MsG0480019935.01.T01:cds;Parent=MsG0480019935.01.T01 |
| Gene Sequence |
| Protein sequence |
