AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017992.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017992.01.T01 MTR_3g116440 84.985 333 41 3 1 330 33 359 0.0 520
MsG0380017992.01.T01 MTR_3g034610 64.489 352 102 3 1 329 1 352 3.91e-143 410
MsG0380017992.01.T01 MTR_3g034610 69.258 283 81 2 53 329 122 404 1.55e-129 376
MsG0380017992.01.T01 MTR_3g034640 51.813 386 122 5 1 328 33 412 1.44e-113 338
MsG0380017992.01.T01 MTR_3g034640 60.638 282 105 2 53 328 209 490 8.40e-108 323
MsG0380017992.01.T01 MTR_3g034640 62.222 270 96 2 65 328 169 438 7.57e-107 321
MsG0380017992.01.T01 MTR_3g034640 60.227 264 103 1 47 308 229 492 2.81e-100 304
MsG0380017992.01.T01 MTR_3g034640 54.639 97 40 1 237 329 57 153 1.82e-20 92.4
MsG0380017992.01.T01 MTR_3g116430 56.125 351 127 6 1 328 33 379 1.16e-112 333
MsG0380017992.01.T01 MTR_3g116430 58.077 260 106 2 47 305 173 430 2.35e-89 274
MsG0380017992.01.T01 MTR_3g107810 49.102 334 148 4 1 328 33 350 9.15e-93 284
MsG0380017992.01.T01 MTR_3g107810 49.286 280 140 2 53 330 199 478 7.21e-80 251
MsG0380017992.01.T01 MTR_4g069810 35.440 364 180 17 1 328 34 378 4.93e-43 152
MsG0380017992.01.T01 MTR_4g069810 37.799 209 120 7 127 330 77 280 6.71e-32 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGTTCAAAATCTTTAT+TGG 0.220637 3:-102960232 None:intergenic
TCTTGGTTCAAAATCTTTAT+TGG 0.225126 3:-102960310 None:intergenic
TCTTGGTTCAAAATCTTTAT+TGG 0.225126 3:-102960388 None:intergenic
AGACCTAACATTTCAGCTTA+TGG 0.239620 3:+102960564 MsG0380017992.01.T01:CDS
AGACCCAATGTTTCAGCTTA+TGG 0.248284 3:+102960018 MsG0380017992.01.T01:CDS
AGACCCAATGTTTCAGCTTA+TGG 0.248284 3:+102960174 MsG0380017992.01.T01:CDS
AGACCCAATGTTTCAGCTTA+TGG 0.248284 3:+102960330 MsG0380017992.01.T01:CDS
AGACCTAATGTTTCAGCTTA+TGG 0.249885 3:+102959940 MsG0380017992.01.T01:CDS
GACCTAACATTTCAGCTTAT+GGG 0.310475 3:+102960565 MsG0380017992.01.T01:CDS
AGGTCTTGGTTCAAAATCTT+TGG 0.310679 3:-102960547 None:intergenic
CATTTATGCATCATATTTAG+TGG 0.320826 3:-102960640 None:intergenic
GTTCCATAAGCTGAAACATT+AGG 0.327800 3:-102959943 None:intergenic
AAACAATTACATTGATGTTA+TGG 0.330758 3:+102960509 MsG0380017992.01.T01:CDS
ATATTTAGTGGCACTTGGTC+GGG 0.347002 3:-102960628 None:intergenic
AGACAATCGTGTTGAAGTTA+AGG 0.379767 3:+102960119 MsG0380017992.01.T01:CDS
AGACAATGGTATTGATGTTA+AGG 0.392683 3:+102960197 MsG0380017992.01.T01:CDS
AGACAATGGTATTGATGTTA+AGG 0.392683 3:+102960353 MsG0380017992.01.T01:CDS
ATTGATGTTAAGGAAAATAA+AGG 0.395870 3:+102960207 MsG0380017992.01.T01:CDS
ATTGATGTTAAGGAAAATAA+AGG 0.395870 3:+102960363 MsG0380017992.01.T01:CDS
AGACCCAATGTTTCAGCATA+TGG 0.405735 3:+102960408 MsG0380017992.01.T01:CDS
ATGAATGATGTCATTGTCTA+TGG 0.405969 3:-102959806 None:intergenic
ATTGATGTTACAGAAAATAA+AGG 0.415243 3:+102960285 MsG0380017992.01.T01:CDS
ACACCCAATGTTTCAGCATA+TGG 0.418154 3:+102960096 MsG0380017992.01.T01:CDS
TCTCCATATGCTGAAATGTT+TGG 0.423109 3:-102960255 None:intergenic
TATGCTGAAACATTGGGTCT+TGG 0.433141 3:-102960483 None:intergenic
TAAGCTGAAACATTGGGTCT+TGG 0.435355 3:-102960171 None:intergenic
TATGCTGAAATGTTTGGTCT+TGG 0.439257 3:-102960249 None:intergenic
GACAATCGTGTTGAAGTTAA+GGG 0.442091 3:+102960120 MsG0380017992.01.T01:CDS
AGACCAAACATTTCAGCATA+TGG 0.445583 3:+102960252 MsG0380017992.01.T01:CDS
CATATTTAGTGGCACTTGGT+CGG 0.446372 3:-102960629 None:intergenic
GTCTCCATATGCTGAAACAT+TGG 0.449066 3:-102960412 None:intergenic
TAAGCTGAAATGTTAGGTCT+TGG 0.458122 3:-102960561 None:intergenic
AAACCTTTGAAAGACTGCTT+CGG 0.462349 3:-102959739 None:intergenic
TAAGCTGAAACATTAGGTCT+TGG 0.463292 3:-102959937 None:intergenic
TAAGCTGAAACATTGGGTCT+TGG 0.464372 3:-102960015 None:intergenic
TAAGCTGAAACATTGGGTCT+TGG 0.464372 3:-102960327 None:intergenic
TATGAAGATAATAACATTGA+GGG 0.464536 3:+102959886 MsG0380017992.01.T01:CDS
ATTCATGCTAAGAGAAAGAA+AGG 0.472696 3:+102959823 MsG0380017992.01.T01:CDS
TATGCTGAAACATTGGGTCT+TGG 0.475638 3:-102960405 None:intergenic
TCTCCATATGCTGAAACATT+GGG 0.476305 3:-102960411 None:intergenic
AACAAATGAAATTGATGCTA+AGG 0.478202 3:+102959963 MsG0380017992.01.T01:CDS
GTCTCCATAAGCTGAAACAT+TGG 0.487144 3:-102960022 None:intergenic
GTCTCCATAAGCTGAAACAT+TGG 0.487144 3:-102960178 None:intergenic
GTCTCCATAAGCTGAAACAT+TGG 0.487144 3:-102960334 None:intergenic
TCCCCATAAGCTGAAATGTT+AGG 0.487972 3:-102960567 None:intergenic
TATTTAGTGGCACTTGGTCG+GGG 0.498218 3:-102960627 None:intergenic
TCTCCATAAGCTGAAACATT+GGG 0.499953 3:-102960021 None:intergenic
TCTCCATAAGCTGAAACATT+GGG 0.499953 3:-102960177 None:intergenic
TCTCCATAAGCTGAAACATT+GGG 0.499953 3:-102960333 None:intergenic
GTCTCCATATGCTGAAACAT+TGG 0.510420 3:-102960100 None:intergenic
GCATCATATTTAGTGGCACT+TGG 0.527772 3:-102960633 None:intergenic
AATTGATGCTAAGGAAAAGA+AGG 0.533314 3:+102959972 MsG0380017992.01.T01:CDS
ACTCCGAAGCAGTCTTTCAA+AGG 0.542773 3:+102959736 MsG0380017992.01.T01:CDS
TCTCCATATGCTGAAACATT+GGG 0.551421 3:-102960099 None:intergenic
ACCTAACATTTCAGCTTATG+GGG 0.562671 3:+102960566 MsG0380017992.01.T01:CDS
GACATGCGAAAAGAAATTCA+AGG 0.567894 3:+102959679 MsG0380017992.01.T01:CDS
GTTTCAGCTTATGGAGACAA+TGG 0.577650 3:+102960183 MsG0380017992.01.T01:CDS
GTTTCAGCTTATGGAGACAA+TGG 0.577650 3:+102960339 MsG0380017992.01.T01:CDS
ATATGAAGATAATAACATTG+AGG 0.587671 3:+102959885 MsG0380017992.01.T01:CDS
TATGCTGAAACATTGGGTGT+TGG 0.621863 3:-102960093 None:intergenic
ACATGCGAAAAGAAATTCAA+GGG 0.625015 3:+102959680 MsG0380017992.01.T01:CDS
TATTGATGTTGAATTCACAA+AGG 0.628277 3:+102960596 MsG0380017992.01.T01:CDS
ACTTGTGTGTCTATTACAAG+TGG 0.633990 3:-102959781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAATTACATTGATGTTA+TGG + Chr3:102960509-102960528 MsG0380017992.01.T01:CDS 20.0%
!! ATATGAAGATAATAACATTG+AGG + Chr3:102959885-102959904 MsG0380017992.01.T01:CDS 20.0%
!! ATTGATGTTAAGGAAAATAA+AGG + Chr3:102960207-102960226 MsG0380017992.01.T01:CDS 20.0%
!! ATTGATGTTACAGAAAATAA+AGG + Chr3:102960285-102960304 MsG0380017992.01.T01:CDS 20.0%
!! TATGAAGATAATAACATTGA+GGG + Chr3:102959886-102959905 MsG0380017992.01.T01:CDS 20.0%
!!! ATCTTCATATTTTGAAACAT+TGG - Chr3:102959875-102959894 None:intergenic 20.0%
!!! TCTTCATATTTTGAAACATT+GGG - Chr3:102959874-102959893 None:intergenic 20.0%
! AACAAATGAAATTGATGCTA+AGG + Chr3:102959963-102959982 MsG0380017992.01.T01:CDS 25.0%
! TATTGATGCTAAGAAAAAGA+AGG + Chr3:102960050-102960069 MsG0380017992.01.T01:CDS 25.0%
! TATTGATGTTGAATTCACAA+AGG + Chr3:102960596-102960615 MsG0380017992.01.T01:CDS 25.0%
! TCTTGGTTCAAAATCTTTAT+TGG - Chr3:102960235-102960254 None:intergenic 25.0%
!! TTTTCATATGCTGAAACATT+GGG - Chr3:102960492-102960511 None:intergenic 25.0%
AATTGATGCTAAGGAAAAGA+AGG + Chr3:102959972-102959991 MsG0380017992.01.T01:CDS 30.0%
ACATGCGAAAAGAAATTCAA+GGG + Chr3:102959680-102959699 MsG0380017992.01.T01:CDS 30.0%
ATGAATGATGTCATTGTCTA+TGG - Chr3:102959809-102959828 None:intergenic 30.0%
ATTCATGCTAAGAGAAAGAA+AGG + Chr3:102959823-102959842 MsG0380017992.01.T01:CDS 30.0%
! GTTTTCATATGCTGAAACAT+TGG - Chr3:102960493-102960512 None:intergenic 30.0%
!! AGACAATGGTATTGATGTTA+AGG + Chr3:102960197-102960216 MsG0380017992.01.T01:CDS 30.0%
!!! TATTTTGAAACATTGGGTCT+TGG - Chr3:102959868-102959887 None:intergenic 30.0%
ACCTAACATTTCAGCTTATG+GGG + Chr3:102960566-102960585 MsG0380017992.01.T01:CDS 35.0%
ACTTGTGTGTCTATTACAAG+TGG - Chr3:102959784-102959803 None:intergenic 35.0%
AGACAATCGTGTTGAAGTTA+AGG + Chr3:102960119-102960138 MsG0380017992.01.T01:CDS 35.0%
AGACCAAACATTTCAGCATA+TGG + Chr3:102960252-102960271 MsG0380017992.01.T01:CDS 35.0%
AGACCTAACATTTCAGCTTA+TGG + Chr3:102960564-102960583 MsG0380017992.01.T01:CDS 35.0%
AGACCTAATGTTTCAGCTTA+TGG + Chr3:102959940-102959959 MsG0380017992.01.T01:CDS 35.0%
AGGTCTTGGTTCAAAATCTT+TGG - Chr3:102960550-102960569 None:intergenic 35.0%
GACAATCGTGTTGAAGTTAA+GGG + Chr3:102960120-102960139 MsG0380017992.01.T01:CDS 35.0%
GACATGCGAAAAGAAATTCA+AGG + Chr3:102959679-102959698 MsG0380017992.01.T01:CDS 35.0%
GACCTAACATTTCAGCTTAT+GGG + Chr3:102960565-102960584 MsG0380017992.01.T01:CDS 35.0%
GTTCCATAAGCTGAAACATT+AGG - Chr3:102959946-102959965 None:intergenic 35.0%
TAAGCTGAAACATTAGGTCT+TGG - Chr3:102959940-102959959 None:intergenic 35.0%
TAAGCTGAAATGTTAGGTCT+TGG - Chr3:102960564-102960583 None:intergenic 35.0%
TATGCTGAAATGTTTGGTCT+TGG - Chr3:102960252-102960271 None:intergenic 35.0%
TCTCCATAAGCTGAAACATT+GGG - Chr3:102960024-102960043 None:intergenic 35.0%
TCTCCATATGCTGAAACATT+GGG - Chr3:102960102-102960121 None:intergenic 35.0%
TCTCCATATGCTGAAATGTT+TGG - Chr3:102960258-102960277 None:intergenic 35.0%
! AAACCTTTGAAAGACTGCTT+CGG - Chr3:102959742-102959761 None:intergenic 35.0%
!! CTTGAGATTCTTTTTCGTCT+CGG - Chr3:102959716-102959735 None:intergenic 35.0%
ACACCCAATGTTTCAGCATA+TGG + Chr3:102960096-102960115 MsG0380017992.01.T01:CDS 40.0%
AGACCCAATGTTTCAGCATA+TGG + Chr3:102960408-102960427 MsG0380017992.01.T01:CDS 40.0%
AGACCCAATGTTTCAGCTTA+TGG + Chr3:102960018-102960037 MsG0380017992.01.T01:CDS 40.0%
ATATTTAGTGGCACTTGGTC+GGG - Chr3:102960631-102960650 None:intergenic 40.0%
CATATTTAGTGGCACTTGGT+CGG - Chr3:102960632-102960651 None:intergenic 40.0%
GCATCATATTTAGTGGCACT+TGG - Chr3:102960636-102960655 None:intergenic 40.0%
GTCTCCATAAGCTGAAACAT+TGG - Chr3:102960025-102960044 None:intergenic 40.0%
GTCTCCATATGCTGAAACAT+TGG - Chr3:102960103-102960122 None:intergenic 40.0%
GTTTCAGCTTATGGAGACAA+TGG + Chr3:102960027-102960046 MsG0380017992.01.T01:CDS 40.0%
TAAGCTGAAACATTGGGTCT+TGG - Chr3:102960018-102960037 None:intergenic 40.0%
TATGCTGAAACATTGGGTCT+TGG - Chr3:102960408-102960427 None:intergenic 40.0%
TATGCTGAAACATTGGGTGT+TGG - Chr3:102960096-102960115 None:intergenic 40.0%
TCCCCATAAGCTGAAATGTT+AGG - Chr3:102960570-102960589 None:intergenic 40.0%
ACTCCGAAGCAGTCTTTCAA+AGG + Chr3:102959736-102959755 MsG0380017992.01.T01:CDS 45.0%
TATTTAGTGGCACTTGGTCG+GGG - Chr3:102960630-102960649 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 102959667 102960659 102959667 ID=MsG0380017992.01;Name=MsG0380017992.01
Chr3 mRNA 102959667 102960659 102959667 ID=MsG0380017992.01.T01;Parent=MsG0380017992.01;Name=MsG0380017992.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|330
Chr3 exon 102959667 102960659 102959667 ID=MsG0380017992.01.T01:exon:3074;Parent=MsG0380017992.01.T01
Chr3 CDS 102959667 102960659 102959667 ID=MsG0380017992.01.T01:cds;Parent=MsG0380017992.01.T01
Gene Sequence

>MsG0380017992.01.T01

ATGAAAGATCAAGACATGCGAAAAGAAATTCAAGGGCTTCTTTACACCGAGACGAAAAAGAATCTCAAGACTCCGAAGCAGTCTTTCAAAGGTTTCAAACATGATTGTGAAGAGCCACTTGTAATAGACACACAAGTCGCCATAGACAATGACATCATTCATGCTAAGAGAAAGAAAGGAGTCACTGAAAATTTTAAACCAAGACCCAATGTTTCAAAATATGAAGATAATAACATTGAGGGAAAGAATAAAGTCGTCAAAGACTTTGAACCAAGACCTAATGTTTCAGCTTATGGAACAAATGAAATTGATGCTAAGGAAAAGAAGGAAGTTATTACAGATTTTGAACCAAGACCCAATGTTTCAGCTTATGGAGACAATGATATTGATGCTAAGAAAAAGAAGGAAATCGTTAAAGATTTTGAACCAACACCCAATGTTTCAGCATATGGAGACAATCGTGTTGAAGTTAAGGGAAAGAAGAAAGACGTTAAAGATTTCAAACCAAGACCCAATGTTTCAGCTTATGGAGACAATGGTATTGATGTTAAGGAAAATAAAGGAGCCAATAAAGATTTTGAACCAAGACCAAACATTTCAGCATATGGAGACAATAACATTGATGTTACAGAAAATAAAGGAGCCAATAAAGATTTTGAACCAAGACCCAATGTTTCAGCTTATGGAGACAATGGTATTGATGTTAAGGAAAATAAAGGAGCCAATAAAGATTTTGAACCAAGACCCAATGTTTCAGCATATGGAGACAATGATATCGATGTTACAGAAAATAAAGAAGTCAATAAAGATTTTGAACCAAGACCCAATGTTTCAGCATATGAAAACAATTACATTGATGTTATGGAAAAGAAGAACGTTACCAAAGATTTTGAACCAAGACCTAACATTTCAGCTTATGGGGACAACAATATTGATGTTGAATTCACAAAGGATTTTGAACCCCGACCAAGTGCCACTAAATATGATGCATAA

Protein sequence

>MsG0380017992.01.T01

MKDQDMRKEIQGLLYTETKKNLKTPKQSFKGFKHDCEEPLVIDTQVAIDNDIIHAKRKKGVTENFKPRPNVSKYEDNNIEGKNKVVKDFEPRPNVSAYGTNEIDAKEKKEVITDFEPRPNVSAYGDNDIDAKKKKEIVKDFEPTPNVSAYGDNRVEVKGKKKDVKDFKPRPNVSAYGDNGIDVKENKGANKDFEPRPNISAYGDNNIDVTENKGANKDFEPRPNVSAYGDNGIDVKENKGANKDFEPRPNVSAYGDNDIDVTENKEVNKDFEPRPNVSAYENNYIDVMEKKNVTKDFEPRPNISAYGDNNIDVEFTKDFEPRPSATKYDA*