AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022242.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022242.01.T01 MTR_4g082883 77.333 75 6 1 11 74 26 100 2.49e-32 107
MsG0480022242.01.T01 MTR_4g082900 55.172 58 26 0 16 73 24 81 9.11e-15 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGGTTTGGCTGGCTCTTC+TGG 0.238096 4:-70058462 None:intergenic
TTTGGCTGGCTCTTCTGGTT+TGG 0.325140 4:-70058457 None:intergenic
CTGGCTCTTCTGGTTTGGAC+TGG 0.372877 4:-70058452 None:intergenic
ACCAATCACACCTTCGGGTT+TGG 0.373855 4:-70058475 None:intergenic
AGCACCACCAATCACACCTT+CGG 0.387425 4:-70058481 None:intergenic
GCTTCAGCACAAACAACTAT+AGG 0.435825 4:+70058366 MsG0480022242.01.T01:CDS
GCACCACCAATCACACCTTC+GGG 0.442267 4:-70058480 None:intergenic
AAAGAAACAATGGCATCAAC+TGG 0.442857 4:+70058303 None:intergenic
ATCACACCTTCGGGTTTGGC+TGG 0.446616 4:-70058471 None:intergenic
GCCAAACCCGAAGGTGTGAT+TGG 0.484923 4:+70058474 MsG0480022242.01.T01:CDS
CTGGACAAAATCAGCAGCCT+TGG 0.492880 4:-70058433 None:intergenic
CTTGGTAGCATACTCTCCTA+CGG 0.606883 4:-70058415 None:intergenic
GAAGAGCCAGCCAAACCCGA+AGG 0.622241 4:+70058465 MsG0480022242.01.T01:CDS
CGTAGGAGAGTATGCTACCA+AGG 0.632082 4:+70058416 MsG0480022242.01.T01:CDS
GCTAATGTTCAGAAGACCGT+AGG 0.649202 4:+70058399 MsG0480022242.01.T01:CDS
AAACCCGAAGGTGTGATTGG+TGG 0.660272 4:+70058477 MsG0480022242.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACCTGCCAAAAAATCGTTAT+TGG - Chr4:70058331-70058350 None:intergenic 35.0%
CCTGCCAAAAAATCGTTATT+GGG - Chr4:70058330-70058349 None:intergenic 35.0%
!!! TGGACCCAATAACGATTTTT+TGG + Chr4:70058323-70058342 MsG0480022242.01.T01:CDS 35.0%
GCTTCAGCACAAACAACTAT+AGG + Chr4:70058366-70058385 MsG0480022242.01.T01:CDS 40.0%
!!! CCCAATAACGATTTTTTGGC+AGG + Chr4:70058327-70058346 MsG0480022242.01.T01:CDS 40.0%
GCTAATGTTCAGAAGACCGT+AGG + Chr4:70058399-70058418 MsG0480022242.01.T01:CDS 45.0%
GGTGCTAAAAATGCTGTTGG+AGG + Chr4:70058498-70058517 MsG0480022242.01.T01:CDS 45.0%
! CTTGGTAGCATACTCTCCTA+CGG - Chr4:70058418-70058437 None:intergenic 45.0%
! GGTGGTGCTAAAAATGCTGT+TGG + Chr4:70058495-70058514 MsG0480022242.01.T01:CDS 45.0%
!!! CGATTTTTTGGCAGGTGCAA+AGG + Chr4:70058335-70058354 MsG0480022242.01.T01:CDS 45.0%
AGCACCACCAATCACACCTT+CGG - Chr4:70058484-70058503 None:intergenic 50.0%
CTGGACAAAATCAGCAGCCT+TGG - Chr4:70058436-70058455 None:intergenic 50.0%
! AAACCCGAAGGTGTGATTGG+TGG + Chr4:70058477-70058496 MsG0480022242.01.T01:CDS 50.0%
! ACCAATCACACCTTCGGGTT+TGG - Chr4:70058478-70058497 None:intergenic 50.0%
! CGTAGGAGAGTATGCTACCA+AGG + Chr4:70058416-70058435 MsG0480022242.01.T01:CDS 50.0%
! TTTGGCTGGCTCTTCTGGTT+TGG - Chr4:70058460-70058479 None:intergenic 50.0%
GCACCACCAATCACACCTTC+GGG - Chr4:70058483-70058502 None:intergenic 55.0%
! ATCACACCTTCGGGTTTGGC+TGG - Chr4:70058474-70058493 None:intergenic 55.0%
! GCCAAACCCGAAGGTGTGAT+TGG + Chr4:70058474-70058493 MsG0480022242.01.T01:CDS 55.0%
!! CTGGCTCTTCTGGTTTGGAC+TGG - Chr4:70058455-70058474 None:intergenic 55.0%
GAAGAGCCAGCCAAACCCGA+AGG + Chr4:70058465-70058484 MsG0480022242.01.T01:CDS 60.0%
! TCGGGTTTGGCTGGCTCTTC+TGG - Chr4:70058465-70058484 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70058312 70058536 70058312 ID=MsG0480022242.01;Name=MsG0480022242.01
Chr4 mRNA 70058312 70058536 70058312 ID=MsG0480022242.01.T01;Parent=MsG0480022242.01;Name=MsG0480022242.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|74
Chr4 exon 70058312 70058536 70058312 ID=MsG0480022242.01.T01:exon:3360;Parent=MsG0480022242.01.T01
Chr4 CDS 70058312 70058536 70058312 ID=MsG0480022242.01.T01:cds;Parent=MsG0480022242.01.T01
Gene Sequence

>MsG0480022242.01.T01

ATGGCATCAACTGGACCCAATAACGATTTTTTGGCAGGTGCAAAGGATGCAGCAGCTTCAGCACAAACAACTATAGGAGATGCTGCAGCTAATGTTCAGAAGACCGTAGGAGAGTATGCTACCAAGGCTGCTGATTTTGTCCAGTCCAAACCAGAAGAGCCAGCCAAACCCGAAGGTGTGATTGGTGGTGCTAAAAATGCTGTTGGAGGCTTATTTAAGAAATGA

Protein sequence

>MsG0480022242.01.T01

MASTGPNNDFLAGAKDAAASAQTTIGDAAANVQKTVGEYATKAADFVQSKPEEPAKPEGVIGGAKNAVGGLFKK*