Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046822.01.T01 | XP_003609412.1 | 97.101 | 69 | 2 | 0 | 1 | 69 | 477 | 545 | 1.23E-36 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046822.01.T01 | G7JFD3 | 97.101 | 69 | 2 | 0 | 1 | 69 | 477 | 545 | 5.88e-37 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048345.01 | MsG0880046822.01 | 0.806660 | 7.383612e-50 | 4.030256e-47 |
| MsG0080048347.01 | MsG0880046822.01 | 0.822985 | 1.767255e-53 | 1.502384e-50 |
| MsG0080048348.01 | MsG0880046822.01 | 0.802416 | 5.669453e-49 | 2.775731e-46 |
| MsG0080048349.01 | MsG0880046822.01 | 0.815209 | 1.039302e-51 | 7.120155e-49 |
| MsG0080048352.01 | MsG0880046822.01 | 0.833253 | 5.932043e-56 | 6.793640e-53 |
| MsG0080049066.01 | MsG0880046822.01 | -0.815114 | 1.091054e-51 | 7.455375e-49 |
| MsG0880044339.01 | MsG0880046822.01 | -0.802469 | 5.527410e-49 | 2.709765e-46 |
| MsG0880045385.01 | MsG0880046822.01 | -0.802598 | 5.200400e-49 | 2.557794e-46 |
| MsG0880045683.01 | MsG0880046822.01 | 0.807453 | 5.017797e-50 | 2.795999e-47 |
| MsG0880046341.01 | MsG0880046822.01 | -0.805508 | 1.290526e-49 | 6.839575e-47 |
| MsG0880046469.01 | MsG0880046822.01 | -0.817288 | 3.564093e-52 | 2.584828e-49 |
| MsG0880046822.01 | MsG0880047493.01 | 0.811243 | 7.718935e-51 | 4.753434e-48 |
| MsG0180003687.01 | MsG0880046822.01 | 0.822505 | 2.285716e-53 | 1.916822e-50 |
| MsG0180003757.01 | MsG0880046822.01 | -0.818964 | 1.488703e-52 | 1.130772e-49 |
| MsG0180005356.01 | MsG0880046822.01 | 0.811640 | 6.327792e-51 | 3.937592e-48 |
| MsG0180005357.01 | MsG0880046822.01 | 0.822446 | 2.359026e-53 | 1.975011e-50 |
| MsG0180005358.01 | MsG0880046822.01 | 0.807935 | 3.963299e-50 | 2.236223e-47 |
| MsG0780041556.01 | MsG0880046822.01 | 0.838564 | 2.668722e-57 | 3.588188e-54 |
| MsG0780041620.01 | MsG0880046822.01 | -0.801439 | 8.999467e-49 | 4.297902e-46 |
| MsG0780041663.01 | MsG0880046822.01 | 0.800124 | 1.669543e-48 | 7.712142e-46 |
| MsG0780041720.01 | MsG0880046822.01 | -0.824980 | 6.016227e-54 | 5.412898e-51 |
| MsG0780041783.01 | MsG0880046822.01 | -0.814057 | 1.870429e-51 | 1.242131e-48 |
| MsG0880042858.01 | MsG0880046822.01 | -0.825259 | 5.167233e-54 | 4.686044e-51 |
| MsG0880043828.01 | MsG0880046822.01 | 0.813491 | 2.491613e-51 | 1.629578e-48 |
| MsG0280010780.01 | MsG0880046822.01 | -0.807641 | 4.578075e-50 | 2.563301e-47 |
| MsG0280011299.01 | MsG0880046822.01 | -0.814440 | 1.538714e-51 | 1.032549e-48 |
| MsG0280011301.01 | MsG0880046822.01 | -0.808044 | 3.758315e-50 | 2.126652e-47 |
| MsG0380013114.01 | MsG0880046822.01 | -0.817566 | 3.085081e-52 | 2.254604e-49 |
| MsG0380013473.01 | MsG0880046822.01 | -0.800392 | 1.472329e-48 | 6.847647e-46 |
| MsG0680031813.01 | MsG0880046822.01 | -0.814473 | 1.513268e-51 | 1.016405e-48 |
| MsG0680034229.01 | MsG0880046822.01 | -0.824739 | 6.856002e-54 | 6.126273e-51 |
| MsG0680035584.01 | MsG0880046822.01 | -0.806638 | 7.463804e-50 | 4.071648e-47 |
| MsG0480018628.01 | MsG0880046822.01 | -0.821191 | 4.603302e-53 | 3.719625e-50 |
| MsG0480019295.01 | MsG0880046822.01 | 0.817548 | 3.115476e-52 | 2.275684e-49 |
| MsG0480019726.01 | MsG0880046822.01 | 0.808633 | 2.813819e-50 | 1.617018e-47 |
| MsG0480020958.01 | MsG0880046822.01 | 0.811313 | 7.451616e-51 | 4.597169e-48 |
| MsG0480021327.01 | MsG0880046822.01 | -0.818092 | 2.348010e-52 | 1.740760e-49 |
| MsG0480021331.01 | MsG0880046822.01 | -0.810818 | 9.539234e-51 | 5.808859e-48 |
| MsG0480022765.01 | MsG0880046822.01 | -0.806215 | 9.166257e-50 | 4.947212e-47 |
| MsG0480023152.01 | MsG0880046822.01 | -0.804735 | 1.872903e-49 | 9.731785e-47 |
| MsG0480023187.01 | MsG0880046822.01 | -0.805846 | 1.096036e-49 | 5.859839e-47 |
| MsG0580024999.01 | MsG0880046822.01 | 0.806422 | 8.288245e-50 | 4.496754e-47 |
| MsG0380015629.01 | MsG0880046822.01 | 0.813444 | 2.551553e-51 | 1.666626e-48 |
| MsG0380015752.01 | MsG0880046822.01 | -0.810642 | 1.041473e-50 | 6.312133e-48 |
| MsG0380016056.01 | MsG0880046822.01 | -0.800229 | 1.589770e-48 | 7.363107e-46 |
| MsG0380016931.01 | MsG0880046822.01 | -0.809260 | 2.065768e-50 | 1.207149e-47 |
| MsG0380016935.01 | MsG0880046822.01 | -0.804401 | 2.198546e-49 | 1.132465e-46 |
| MsG0380017405.01 | MsG0880046822.01 | -0.809588 | 1.757141e-50 | 1.035840e-47 |
| MsG0380017690.01 | MsG0880046822.01 | 0.814824 | 1.265674e-51 | 8.581279e-49 |
| MsG0380018026.01 | MsG0880046822.01 | 0.804455 | 2.142140e-49 | 1.104966e-46 |
| MsG0580027733.01 | MsG0880046822.01 | 0.812837 | 3.467801e-51 | 2.227989e-48 |
| MsG0580028999.01 | MsG0880046822.01 | 0.811847 | 5.704565e-51 | 3.569458e-48 |
| MsG0280006883.01 | MsG0880046822.01 | 0.808653 | 2.785799e-50 | 1.601754e-47 |
| MsG0280007650.01 | MsG0880046822.01 | -0.802552 | 5.313572e-49 | 2.610390e-46 |
| MsG0280008405.01 | MsG0880046822.01 | 0.811823 | 5.773681e-51 | 3.610442e-48 |
| MsG0280008912.01 | MsG0880046822.01 | -0.810469 | 1.134819e-50 | 6.846260e-48 |
| MsG0280009742.01 | MsG0880046822.01 | -0.807704 | 4.439226e-50 | 2.489668e-47 |
| MsG0680035841.01 | MsG0880046822.01 | 0.827542 | 1.477372e-54 | 1.430512e-51 |
| MsG0780036661.01 | MsG0880046822.01 | 0.804554 | 2.043447e-49 | 1.056730e-46 |
| MsG0780037751.01 | MsG0880046822.01 | 0.803289 | 3.742394e-49 | 1.873397e-46 |
| MsG0780039821.01 | MsG0880046822.01 | -0.803321 | 3.686627e-49 | 1.846970e-46 |
| MsG0780039839.01 | MsG0880046822.01 | -0.803785 | 2.952928e-49 | 1.497246e-46 |
| MsG0780039968.01 | MsG0880046822.01 | 0.801709 | 7.921505e-49 | 3.808944e-46 |
| MsG0780040144.01 | MsG0880046822.01 | 0.808131 | 3.600975e-50 | 2.042396e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046822.01.T01 | MTR_4g115420 | 97.101 | 69 | 2 | 0 | 1 | 69 | 477 | 545 | 1.49e-40 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAATCTGCAAATCCTTT+TGG | 0.269633 | 8:-78757289 | MsG0880046822.01.T01:CDS |
| GATACATGAAAGACTGTTGC+TGG | 0.435439 | 8:+78757350 | None:intergenic |
| TGCTCCACACTCTGTTCAAA+TGG | 0.437973 | 8:-78757386 | MsG0880046822.01.T01:CDS |
| ACAGTTCACCCCTATGGCTC+AGG | 0.500843 | 8:-78757247 | MsG0880046822.01.T01:CDS |
| GTTTAGATGAGGCCTGTGTA+AGG | 0.501347 | 8:+78757202 | None:intergenic |
| TAAGGATTGTATGATTGAAC+AGG | 0.502702 | 8:+78757220 | None:intergenic |
| CCATATTGAGGATTTCCAAA+AGG | 0.508316 | 8:+78757274 | None:intergenic |
| GGATTTGCAGATTGTTGTTG+AGG | 0.508477 | 8:+78757295 | None:intergenic |
| GGAGCCACAGTTCACCCCTA+TGG | 0.518635 | 8:-78757253 | MsG0880046822.01.T01:CDS |
| TGAACTGTGGCTCCATATTG+AGG | 0.535591 | 8:+78757262 | None:intergenic |
| GATTTGCAGATTGTTGTTGA+GGG | 0.546331 | 8:+78757296 | None:intergenic |
| AACAGGCATACCTGAGCCAT+AGG | 0.555181 | 8:+78757237 | None:intergenic |
| GCAGCAACAAATGATGATGA+TGG | 0.566303 | 8:-78757320 | MsG0880046822.01.T01:CDS |
| CCACAAGAAATGTTTAGATG+AGG | 0.593524 | 8:+78757191 | None:intergenic |
| ACAGGCATACCTGAGCCATA+GGG | 0.603728 | 8:+78757238 | None:intergenic |
| ATTTGCAGATTGTTGTTGAG+GGG | 0.604944 | 8:+78757297 | None:intergenic |
| CAGGCATACCTGAGCCATAG+GGG | 0.630660 | 8:+78757239 | None:intergenic |
| GCTGCCATTTGAACAGAGTG+TGG | 0.639866 | 8:+78757382 | None:intergenic |
| CAATCATACAATCCTTACAC+AGG | 0.659165 | 8:-78757214 | MsG0880046822.01.T01:CDS |
| TGAGCCATAGGGGTGAACTG+TGG | 0.722937 | 8:+78757249 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAGGATTGTATGATTGAAC+AGG | + | Chr8:78757378-78757397 | None:intergenic | 30.0% | |
| CAATCATACAATCCTTACAC+AGG | - | Chr8:78757381-78757400 | MsG0880046822.01.T01:CDS | 35.0% | |
| CCATATTGAGGATTTCCAAA+AGG | + | Chr8:78757324-78757343 | None:intergenic | 35.0% | |
| ! | ATTTGCAGATTGTTGTTGAG+GGG | + | Chr8:78757301-78757320 | None:intergenic | 35.0% |
| ! | CAACAATCTGCAAATCCTTT+TGG | - | Chr8:78757306-78757325 | MsG0880046822.01.T01:CDS | 35.0% |
| ! | GATTTGCAGATTGTTGTTGA+GGG | + | Chr8:78757302-78757321 | None:intergenic | 35.0% |
| !! | CCTTTTGGAAATCCTCAATA+TGG | - | Chr8:78757321-78757340 | MsG0880046822.01.T01:CDS | 35.0% |
| GATACATGAAAGACTGTTGC+TGG | + | Chr8:78757248-78757267 | None:intergenic | 40.0% | |
| ! | GCAGCAACAAATGATGATGA+TGG | - | Chr8:78757275-78757294 | MsG0880046822.01.T01:CDS | 40.0% |
| ! | GGATTTGCAGATTGTTGTTG+AGG | + | Chr8:78757303-78757322 | None:intergenic | 40.0% |
| TGAACTGTGGCTCCATATTG+AGG | + | Chr8:78757336-78757355 | None:intergenic | 45.0% | |
| TGCTCCACACTCTGTTCAAA+TGG | - | Chr8:78757209-78757228 | MsG0880046822.01.T01:CDS | 45.0% | |
| AACAGGCATACCTGAGCCAT+AGG | + | Chr8:78757361-78757380 | None:intergenic | 50.0% | |
| ACAGGCATACCTGAGCCATA+GGG | + | Chr8:78757360-78757379 | None:intergenic | 50.0% | |
| ! | GCTGCCATTTGAACAGAGTG+TGG | + | Chr8:78757216-78757235 | None:intergenic | 50.0% |
| ACAGTTCACCCCTATGGCTC+AGG | - | Chr8:78757348-78757367 | MsG0880046822.01.T01:CDS | 55.0% | |
| CAGGCATACCTGAGCCATAG+GGG | + | Chr8:78757359-78757378 | None:intergenic | 55.0% | |
| TGAGCCATAGGGGTGAACTG+TGG | + | Chr8:78757349-78757368 | None:intergenic | 55.0% | |
| GGAGCCACAGTTCACCCCTA+TGG | - | Chr8:78757342-78757361 | MsG0880046822.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 78757204 | 78757413 | 78757204 | ID=MsG0880046822.01;Name=MsG0880046822.01 |
| Chr8 | mRNA | 78757204 | 78757413 | 78757204 | ID=MsG0880046822.01.T01;Parent=MsG0880046822.01;Name=MsG0880046822.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|69 |
| Chr8 | exon | 78757204 | 78757413 | 78757204 | ID=MsG0880046822.01.T01:exon:18908;Parent=MsG0880046822.01.T01 |
| Chr8 | CDS | 78757204 | 78757413 | 78757204 | ID=MsG0880046822.01.T01:cds;Parent=MsG0880046822.01.T01 |
| Gene Sequence |
| Protein sequence |
