AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041620.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041620.01.T01 MTR_7g114590 98.361 183 3 0 1 183 1 183 3.47e-135 375
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041620.01.T01 AT1G54500 62.121 198 57 7 1 183 1 195 9.03e-65 197

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAACTGTGCTTATTTA+TGG 0.158905 7:-93162354 MsG0780041620.01.T01:CDS
GGGGTTGGTGTAATGGAGTT+TGG 0.242995 7:+93162773 None:intergenic
CTTGGTGGAAATTCTCTTAC+TGG 0.257542 7:-93162381 MsG0780041620.01.T01:CDS
AACTGAAAACCAGGTGGAAT+TGG 0.298992 7:+93162513 None:intergenic
GGGTTGGTGTAATGGAGTTT+GGG 0.326512 7:+93162774 None:intergenic
GGAGTTTGGGTTTGAGAGGA+TGG 0.335353 7:+93162787 None:intergenic
TTGAAAAGCTTCCAGATGAT+TGG 0.347062 7:-93162491 MsG0780041620.01.T01:CDS
TTTGCTCAGAATCAACAATA+TGG 0.354668 7:-93162405 MsG0780041620.01.T01:CDS
AGAGGATGGATTTGAGAGAA+AGG 0.359838 7:+93162801 None:intergenic
TTTGAGTTTGTTGGAGTGAT+GGG 0.360473 7:+93162728 None:intergenic
ATGAAGGGGTTTGAGTTTGT+TGG 0.377386 7:+93162719 None:intergenic
TTTGTTGGAGTGATGGGTTT+TGG 0.390206 7:+93162734 None:intergenic
AAAGGGAGGGGTTGGTGTAA+TGG 0.410099 7:+93162766 None:intergenic
AGTAAAAGTGTTGAAATTGC+TGG 0.415161 7:-93162429 MsG0780041620.01.T01:CDS
CAGTGAAGTTTCGTGGATGA+AGG 0.418966 7:+93162703 None:intergenic
GTTTGAGTTTGTTGGAGTGA+TGG 0.421219 7:+93162727 None:intergenic
AGGAGTTGAGAGATGGAAAG+TGG 0.425190 7:+93162821 None:intergenic
GTGGGACATCTCCAATCATC+TGG 0.448648 7:+93162480 None:intergenic
GAGTTTACAGTGAAGTTTCG+TGG 0.451202 7:+93162696 None:intergenic
CAGAATCAACAATATGGTCT+TGG 0.468077 7:-93162399 MsG0780041620.01.T01:CDS
AAGTTCGCTGTGTTGAATAC+AGG 0.478954 7:-93162597 MsG0780041620.01.T01:CDS
AATCAACAATATGGTCTTGG+TGG 0.481663 7:-93162396 MsG0780041620.01.T01:CDS
GAGAGAAAGGAGTTGAGAGA+TGG 0.493471 7:+93162814 None:intergenic
AGTGAAGTTTCGTGGATGAA+GGG 0.530603 7:+93162704 None:intergenic
CCTTCGTATCCAATTCCACC+TGG 0.534697 7:-93162522 MsG0780041620.01.T01:CDS
TAATGGAGTTTGGGTTTGAG+AGG 0.568113 7:+93162783 None:intergenic
ACTTTGTGCAGCACCACAAG+TGG 0.579838 7:+93162461 None:intergenic
GGTGGAAATTCTCTTACTGG+TGG 0.584178 7:-93162378 MsG0780041620.01.T01:CDS
CCAGGTGGAATTGGATACGA+AGG 0.592505 7:+93162522 None:intergenic
GTGAAGTTTCGTGGATGAAG+GGG 0.593003 7:+93162705 None:intergenic
TATAAATATGATGAAGGTGT+AGG 0.596708 7:-93162549 MsG0780041620.01.T01:CDS
TGTGGTTATAAATATGATGA+AGG 0.597525 7:-93162555 MsG0780041620.01.T01:CDS
ATCTATGAATGTCGTTCATG+TGG 0.599864 7:-93162573 MsG0780041620.01.T01:CDS
GATTGGAGATGTCCCACTTG+TGG 0.648504 7:-93162474 MsG0780041620.01.T01:CDS
CTTTGTGCAGCACCACAAGT+GGG 0.685384 7:+93162462 None:intergenic
CAGGTGGAATTGGATACGAA+GGG 0.702702 7:+93162523 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTGGTTATAAATATGATGA+AGG - Chr7:93162578-93162597 MsG0780041620.01.T01:CDS 25.0%
!! TATAAATATGATGAAGGTGT+AGG - Chr7:93162584-93162603 MsG0780041620.01.T01:CDS 25.0%
CAGAAAACTGTGCTTATTTA+TGG - Chr7:93162779-93162798 MsG0780041620.01.T01:CDS 30.0%
TTTGCTCAGAATCAACAATA+TGG - Chr7:93162728-93162747 MsG0780041620.01.T01:CDS 30.0%
!! AGTAAAAGTGTTGAAATTGC+TGG - Chr7:93162704-93162723 MsG0780041620.01.T01:CDS 30.0%
ATCTATGAATGTCGTTCATG+TGG - Chr7:93162560-93162579 MsG0780041620.01.T01:CDS 35.0%
CAGAATCAACAATATGGTCT+TGG - Chr7:93162734-93162753 MsG0780041620.01.T01:CDS 35.0%
TTGAAAAGCTTCCAGATGAT+TGG - Chr7:93162642-93162661 MsG0780041620.01.T01:CDS 35.0%
! AATCAACAATATGGTCTTGG+TGG - Chr7:93162737-93162756 MsG0780041620.01.T01:CDS 35.0%
! AGCTTTTCAAACTGAAAACC+AGG + Chr7:93162632-93162651 None:intergenic 35.0%
! TTTTCAAACTGAAAACCAGG+TGG + Chr7:93162629-93162648 None:intergenic 35.0%
!! TTTGAGTTTGTTGGAGTGAT+GGG + Chr7:93162408-93162427 None:intergenic 35.0%
!!! GGTTTTGGAAAGGAGAAAAA+GGG + Chr7:93162387-93162406 None:intergenic 35.0%
!!! TTCTTTGCACTTTTTCTCAG+TGG - Chr7:93162815-93162834 MsG0780041620.01.T01:CDS 35.0%
AACTGAAAACCAGGTGGAAT+TGG + Chr7:93162623-93162642 None:intergenic 40.0%
AAGTTCGCTGTGTTGAATAC+AGG - Chr7:93162536-93162555 MsG0780041620.01.T01:CDS 40.0%
AGTGAAGTTTCGTGGATGAA+GGG + Chr7:93162432-93162451 None:intergenic 40.0%
CTTGGTGGAAATTCTCTTAC+TGG - Chr7:93162752-93162771 MsG0780041620.01.T01:CDS 40.0%
GAGTTTACAGTGAAGTTTCG+TGG + Chr7:93162440-93162459 None:intergenic 40.0%
TTGGAAAGGAGAAAAAGGGA+GGG + Chr7:93162383-93162402 None:intergenic 40.0%
TTTGGAAAGGAGAAAAAGGG+AGG + Chr7:93162384-93162403 None:intergenic 40.0%
! AAACGACGTTTTTCTGAGTC+GGG + Chr7:93162509-93162528 None:intergenic 40.0%
! AGAGGATGGATTTGAGAGAA+AGG + Chr7:93162335-93162354 None:intergenic 40.0%
! ATGAAGGGGTTTGAGTTTGT+TGG + Chr7:93162417-93162436 None:intergenic 40.0%
! CAAACGACGTTTTTCTGAGT+CGG + Chr7:93162510-93162529 None:intergenic 40.0%
! TAATGGAGTTTGGGTTTGAG+AGG + Chr7:93162353-93162372 None:intergenic 40.0%
!! GTTTGAGTTTGTTGGAGTGA+TGG + Chr7:93162409-93162428 None:intergenic 40.0%
!!! GGGTTTTGGAAAGGAGAAAA+AGG + Chr7:93162388-93162407 None:intergenic 40.0%
!!! TTTGTTGGAGTGATGGGTTT+TGG + Chr7:93162402-93162421 None:intergenic 40.0%
AGGAGTTGAGAGATGGAAAG+TGG + Chr7:93162315-93162334 None:intergenic 45.0%
CAGGTGGAATTGGATACGAA+GGG + Chr7:93162613-93162632 None:intergenic 45.0%
CAGTGAAGTTTCGTGGATGA+AGG + Chr7:93162433-93162452 None:intergenic 45.0%
CGACTCAGAAAAACGTCGTT+TGG - Chr7:93162508-93162527 MsG0780041620.01.T01:CDS 45.0%
GAGAGAAAGGAGTTGAGAGA+TGG + Chr7:93162322-93162341 None:intergenic 45.0%
GGTGGAAATTCTCTTACTGG+TGG - Chr7:93162755-93162774 MsG0780041620.01.T01:CDS 45.0%
GTGAAGTTTCGTGGATGAAG+GGG + Chr7:93162431-93162450 None:intergenic 45.0%
TGGAAAGGAGAAAAAGGGAG+GGG + Chr7:93162382-93162401 None:intergenic 45.0%
!! GGGTTGGTGTAATGGAGTTT+GGG + Chr7:93162362-93162381 None:intergenic 45.0%
!!! TGGAGTGATGGGTTTTGGAA+AGG + Chr7:93162397-93162416 None:intergenic 45.0%
AAGGAGAAAAAGGGAGGGGT+TGG + Chr7:93162378-93162397 None:intergenic 50.0%
ACTTTGTGCAGCACCACAAG+TGG + Chr7:93162675-93162694 None:intergenic 50.0%
CCAGGTGGAATTGGATACGA+AGG + Chr7:93162614-93162633 None:intergenic 50.0%
CCTTCGTATCCAATTCCACC+TGG - Chr7:93162611-93162630 MsG0780041620.01.T01:CDS 50.0%
CTTTGTGCAGCACCACAAGT+GGG + Chr7:93162674-93162693 None:intergenic 50.0%
GATTGGAGATGTCCCACTTG+TGG - Chr7:93162659-93162678 MsG0780041620.01.T01:CDS 50.0%
GTGGGACATCTCCAATCATC+TGG + Chr7:93162656-93162675 None:intergenic 50.0%
! GGAGTTTGGGTTTGAGAGGA+TGG + Chr7:93162349-93162368 None:intergenic 50.0%
!! AAAGGGAGGGGTTGGTGTAA+TGG + Chr7:93162370-93162389 None:intergenic 50.0%
!! GGGGTTGGTGTAATGGAGTT+TGG + Chr7:93162363-93162382 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 93162302 93162853 93162302 ID=MsG0780041620.01;Name=MsG0780041620.01
Chr7 mRNA 93162302 93162853 93162302 ID=MsG0780041620.01.T01;Parent=MsG0780041620.01;Name=MsG0780041620.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|183
Chr7 exon 93162302 93162853 93162302 ID=MsG0780041620.01.T01:exon:22056;Parent=MsG0780041620.01.T01
Chr7 CDS 93162302 93162853 93162302 ID=MsG0780041620.01.T01:cds;Parent=MsG0780041620.01.T01
Gene Sequence

>MsG0780041620.01.T01

ATGGCTGCTTCCACTTTCCATCTCTCAACTCCTTTCTCTCAAATCCATCCTCTCAAACCCAAACTCCATTACACCAACCCCTCCCTTTTTCTCCTTTCCAAAACCCATCACTCCAACAAACTCAAACCCCTTCATCCACGAAACTTCACTGTAAACTCTGTAGACATCTCAAATGAAGAAGATACAACTTCAGAGTCTGAAAAACCCGACTCAGAAAAACGTCGTTTGGAAGAAAAGTTCGCTGTGTTGAATACAGGAATCTATGAATGTCGTTCATGTGGTTATAAATATGATGAAGGTGTAGGTGACCCTTCGTATCCAATTCCACCTGGTTTTCAGTTTGAAAAGCTTCCAGATGATTGGAGATGTCCCACTTGTGGTGCTGCACAAAGTTTCTTTGAAAGTAAAAGTGTTGAAATTGCTGGTTTTGCTCAGAATCAACAATATGGTCTTGGTGGAAATTCTCTTACTGGTGGTCAGAAAACTGTGCTTATTTATGGTACTTTGTTGTTTTTCTTTGCACTTTTTCTCAGTGGCTACTTCATACAATAG

Protein sequence

>MsG0780041620.01.T01

MAASTFHLSTPFSQIHPLKPKLHYTNPSLFLLSKTHHSNKLKPLHPRNFTVNSVDISNEEDTTSESEKPDSEKRRLEEKFAVLNTGIYECRSCGYKYDEGVGDPSYPIPPGFQFEKLPDDWRCPTCGAAQSFFESKSVEIAGFAQNQQYGLGGNSLTGGQKTVLIYGTLLFFFALFLSGYFIQ*