AlfalfaGEDB | Msa1303040

M. sativa cultivar zhongmu-4 / Msa1303040

KEGG: K00975
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008878 GO:0009501 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0016740 GO:0016772 GO:0016779 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0070566
PFAM: PF00483.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303040	sp\|P55229\|GLGL1_ARATH	78.817	524	106	3	1	521	1	522	0.0	865

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303040	G7JI05	98.081	521	10	0	1	521	1	521	0.0	1065

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1303040	0.868105	8.361561e-66	-8.615850e-47
Msa0001040	Msa1303040	0.832669	8.283535e-56	-8.615850e-47
Msa0001420	Msa1303040	0.810155	1.326534e-50	-8.615850e-47
Msa0001510	Msa1303040	0.880325	6.022562e-70	-8.615850e-47
Msa0001540	Msa1303040	0.856678	2.735667e-62	-8.615850e-47
Msa0001880	Msa1303040	0.800191	1.617782e-48	-8.615850e-47
Msa0002060	Msa1303040	0.834041	3.769956e-56	-8.615850e-47
Msa0002110	Msa1303040	0.817393	3.374774e-52	-8.615850e-47
Msa0002690	Msa1303040	0.914830	1.208665e-84	-8.615850e-47
Msa0002700	Msa1303040	0.913320	7.058530e-84	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303040	MtrunA17_Chr4g0074531	98.081	521	10	0	1	521	1	521	0.0	1065
Msa1303040	MtrunA17_Chr3g0141761	68.397	443	140	0	79	521	88	530	0.0	660
Msa1303040	MtrunA17_Chr4g0035171	64.224	464	162	2	61	521	66	528	0.0	656
Msa1303040	MtrunA17_Chr7g0271011	62.815	476	166	2	57	521	61	536	0.0	632
Msa1303040	MtrunA17_Chr0c30g0494071	69.231	481	37	6	74	521	13	415	0.0	616
Msa1303040	MtrunA17_Chr5g0447341	57.882	425	172	4	99	521	96	515	0.0	518
Msa1303040	MtrunA17_Chr3g0120471	53.785	502	208	9	25	521	29	511	6.32e-180	515
Msa1303040	MtrunA17_Chr3g0144741	69.531	256	77	1	82	337	80	334	1.81e-128	379
Msa1303040	MtrunA17_Chr3g0144731	62.500	160	59	1	362	521	3	161	2.92e-68	216
Msa1303040	MtrunA17_Chr0c30g0494061	93.939	66	4	0	10	75	1	66	2.84e-36	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1303040	AT5G19220.1	78.817	524	106	3	1	521	1	522	865
Msa1303040	AT1G27680.1	67.735	468	151	0	54	521	51	518	687
Msa1303040	AT4G39210.2	61.966	468	173	3	58	521	55	521	628
Msa1303040	AT4G39210.1	61.966	468	173	3	58	521	55	521	628
Msa1303040	AT2G21590.2	63.920	449	159	2	75	521	76	523	614
Msa1303040	AT2G21590.3	63.920	449	159	2	75	521	76	523	614
Msa1303040	AT2G21590.5	63.920	449	159	2	75	521	76	523	614
Msa1303040	AT2G21590.4	63.920	449	159	2	75	521	76	523	614
Msa1303040	AT2G21590.7	63.920	449	159	2	75	521	76	523	614
Msa1303040	AT2G21590.1	63.920	449	159	2	75	521	76	523	614

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Find 139 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCATATATTGCTTCGA+TGG	0.524539	8_3:-65768497	Msa1303040:CDS
AAAATGGCAGTCTCTGCCAG+TGG	0.689914	8_3:-65771164	Msa1303040:CDS
AAAATGGTTCAAACAAATGA+TGG	0.486066	8_3:+65766448	None:intergenic
AAAATTCAACAATTGAAGAC+GGG	0.524972	8_3:-65766492	Msa1303040:CDS
AAACACGGACAAGAAGACAA+AGG	0.716788	8_3:-65771239	Msa1303040:five_prime_UTR
AAACCATATATTGCTTCGAT+GGG	0.560652	8_3:-65768496	Msa1303040:CDS
AAACTCAAATGTTCATTTAA+AGG	0.138712	8_3:-65767239	Msa1303040:intron
AAAGCTCGTCCTCAAATCCG+TGG	0.659004	8_3:+65766424	None:intergenic
AAATGGTTCAAACAAATGAT+GGG	0.563185	8_3:+65766449	None:intergenic
AACACTCTTGGTGCAACCAT+TGG	0.542518	8_3:+65771016	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATCCTATGGATTTAGACT+TGG	+	chr8_3:65766198-65766217	None:intergenic	30.0%
ACAATTGTATTTGGATAACG+TGG	-	chr8_3:65766328-65766347	Msa1303040:three_prime_UTR	30.0%
GGAATTAGAGTTGTTAATGA+AGG	+	chr8_3:65766478-65766497	None:intergenic	30.0%
CAAGAAAGAGTTAATTGCAT+TGG	+	chr8_3:65766792-65766811	None:intergenic	30.0%
GCAATTAACTCTTTCTTGTT+TGG	-	chr8_3:65766794-65766813	Msa1303040:CDS	30.0%
TAGAAACGTTGTGAAATTCT+TGG	-	chr8_3:65766900-65766919	Msa1303040:intron	30.0%
TGACCTATATACTACTTCAA+TGG	-	chr8_3:65767175-65767194	Msa1303040:intron	30.0%
CTATATACTACTTCAATGGT+GGG	-	chr8_3:65767179-65767198	Msa1303040:intron	30.0%
CAAAGAAAATCTTAATGGAG+GGG	+	chr8_3:65767229-65767248	None:intergenic	30.0%
GCAAAGAAAATCTTAATGGA+GGG	+	chr8_3:65767230-65767249	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	65766188	65771524	65766188	ID=Msa1303040;Name=Msa1303040
chr8_3	mRNA	65766188	65771524	65766188	ID=Msa1303040-mRNA-1;Parent=Msa1303040;Name=Msa1303040-mRNA-1;_AED=0.04;_eAED=0.04;_QI=293\|1\|1\|1\|0.93\|0.87\|16\|292\|521
chr8_3	exon	65766188	65766581	65766188	ID=Msa1303040-mRNA-1:exon:19987;Parent=Msa1303040-mRNA-1
chr8_3	exon	65766769	65766829	65766769	ID=Msa1303040-mRNA-1:exon:19986;Parent=Msa1303040-mRNA-1
chr8_3	exon	65766911	65767017	65766911	ID=Msa1303040-mRNA-1:exon:19985;Parent=Msa1303040-mRNA-1
chr8_3	exon	65767240	65767344	65767240	ID=Msa1303040-mRNA-1:exon:19984;Parent=Msa1303040-mRNA-1
chr8_3	exon	65767466	65767552	65767466	ID=Msa1303040-mRNA-1:exon:19983;Parent=Msa1303040-mRNA-1
chr8_3	exon	65767747	65767827	65767747	ID=Msa1303040-mRNA-1:exon:19982;Parent=Msa1303040-mRNA-1
chr8_3	exon	65768230	65768302	65768230	ID=Msa1303040-mRNA-1:exon:19981;Parent=Msa1303040-mRNA-1
chr8_3	exon	65768454	65768566	65768454	ID=Msa1303040-mRNA-1:exon:19980;Parent=Msa1303040-mRNA-1
chr8_3	exon	65768656	65768749	65768656	ID=Msa1303040-mRNA-1:exon:19979;Parent=Msa1303040-mRNA-1
chr8_3	exon	65768863	65768918	65768863	ID=Msa1303040-mRNA-1:exon:19978;Parent=Msa1303040-mRNA-1
chr8_3	exon	65769023	65769106	65769023	ID=Msa1303040-mRNA-1:exon:19977;Parent=Msa1303040-mRNA-1
chr8_3	exon	65769247	65769339	65769247	ID=Msa1303040-mRNA-1:exon:19976;Parent=Msa1303040-mRNA-1
chr8_3	exon	65769480	65769653	65769480	ID=Msa1303040-mRNA-1:exon:19975;Parent=Msa1303040-mRNA-1
chr8_3	exon	65770025	65770138	65770025	ID=Msa1303040-mRNA-1:exon:19974;Parent=Msa1303040-mRNA-1
chr8_3	exon	65770962	65771463	65770962	ID=Msa1303040-mRNA-1:exon:19973;Parent=Msa1303040-mRNA-1
chr8_3	exon	65771512	65771524	65771512	ID=Msa1303040-mRNA-1:exon:19972;Parent=Msa1303040-mRNA-1
chr8_3	five_prime_UTR	65771512	65771524	65771512	ID=Msa1303040-mRNA-1:five_prime_utr;Parent=Msa1303040-mRNA-1
chr8_3	five_prime_UTR	65771184	65771463	65771184	ID=Msa1303040-mRNA-1:five_prime_utr;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65770962	65771183	65770962	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65770025	65770138	65770025	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65769480	65769653	65769480	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65769247	65769339	65769247	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65769023	65769106	65769023	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65768863	65768918	65768863	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65768656	65768749	65768656	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65768454	65768566	65768454	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65768230	65768302	65768230	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65767747	65767827	65767747	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65767466	65767552	65767466	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65767240	65767344	65767240	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65766911	65767017	65766911	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65766769	65766829	65766769	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	CDS	65766480	65766581	65766480	ID=Msa1303040-mRNA-1:cds;Parent=Msa1303040-mRNA-1
chr8_3	three_prime_UTR	65766188	65766479	65766188	ID=Msa1303040-mRNA-1:three_prime_utr;Parent=Msa1303040-mRNA-1

Gene Sequence

>Msa1303040

ATGGCAGTCTCTGCCAGTGGTCAGATAATGCTATCATCTACCGTTCAGCTACGTGAGCCAGGAATGGTGACCAGTAGAAACTTGAAGGTTGTGAAGTTTTGTAATGGAGAAATGATGGGACGAAAGATTGAGCTTCATGCTGCTACCAATGGTTGCACCAAGAGTGTTACTAGGAAAAATATATCCATGTCTCTCACTGCTGATGTGGCTAGCGAATCCAAGTTGAAAAATGCAGATGTGGAAAAAAGGGATCCAAGTACAGTCGTGGCAGTTATACTTGGTGGAGGAGCTGGAACCCGTCTCTTCCCTCTAACTAAGCGACGAGCAAAGCCTGCTGTTCCAATTGGAGGTGCTTATAGGCTGATTGATGTGCCAATGAGTAACTGCATCAATAGTGGGATCAACAAAGTGTATATTCTCACTCAGTTTAATTCAGCCTCACTCAACAGACATATTGCACGTGCTTACAACTCCGGTACTGGAGTCACTTTCGGAGATGGCTATGTCGAGGTTCTTGCTGCAACTCAAACTCCAGGGGAACAAGGTAAAAAGTGGTTTCAAGGTACTGCTGATGCTGTGCGGCAGTTTCACTGGCTCTTTGAGGATCCCAGGAGCAAGGATATTGAGGATGTTTTGATTCTTTCTGGGGATCACCTCTACCGTATGGACTATATGGACTTTGTTAGAGATCATCGAGAGAGCGGTGCAGATATCACTCTTTCTTGTCTGCCAATGGATGACAGCCGCGCCTCAGATTTCGGTCTGATGAAGATTGACAACAAAGGAAGGATCCTTTCATTTAGTGAAAAGCCTAAAGGAGCAGAGCTCAAAGCAATGCAAGTGGATACAACCGTTCTTGGTCTTTCGAAGGATGAAGCTCTAGAAAAACCATATATTGCTTCGATGGGAGTGTACGTCTTCAAGAAGGAGATACTTCTTAATCTATTAAGATGGCGCTTTCCAACTGCAAATGACTTTGGATCAGAGGTCATTCCTGCCTCAGCTAAAGAATTTTACATGAAGGCTTATCTCTTCAACGATTACTGGGAGGACATAGGTACTATCAGATCATTCTTTGAGGCAAATCTAGCCCTCACTGAGCATCCATCCAAGTTTAGCTTTTACGATGCAGCAAAACCTATGTACACATCAAGGAGAAATTTACCACCATCGAAGATTGACAATAGCAAGCTCGTTGATTCAATTATATCACATGGAAGCTTCGTGAATAATGCCTTCATAGAGCATAGTGTAGTTGGAATCAGATCAAGAATAAACTCAAATGTTCATTTAAAGGACACAGTGATGCTTGGAGCTGATTACTATGAAACTGAGGCAGAAGTGGCAGCACAGTTAGCTGAGGGAAGAGTTCCAATAGGAATAGGAGAAAACACCAAAATCAAAGACTGTATTATTGACAAAAATGCTAGAATTGGAAAGAATGTTGTCATAGCAAACTCAGAGGGAGTACAAGAGGCTGATAGATCATCAGAAGGGTTTTACATCCGTTCCGGCGTCACTGTTGTATTGAAAAATTCAACAATTGAAGACGGGCTCGTCATATAA

Protein sequence

>Msa1303040

MAVSASGQIMLSSTVQLREPGMVTSRNLKVVKFCNGEMMGRKIELHAATNGCTKSVTRKNISMSLTADVASESKLKNADVEKRDPSTVVAVILGGGAGTRLFPLTKRRAKPAVPIGGAYRLIDVPMSNCINSGINKVYILTQFNSASLNRHIARAYNSGTGVTFGDGYVEVLAATQTPGEQGKKWFQGTADAVRQFHWLFEDPRSKDIEDVLILSGDHLYRMDYMDFVRDHRESGADITLSCLPMDDSRASDFGLMKIDNKGRILSFSEKPKGAELKAMQVDTTVLGLSKDEALEKPYIASMGVYVFKKEILLNLLRWRFPTANDFGSEVIPASAKEFYMKAYLFNDYWEDIGTIRSFFEANLALTEHPSKFSFYDAAKPMYTSRRNLPPSKIDNSKLVDSIISHGSFVNNAFIEHSVVGIRSRINSNVHLKDTVMLGADYYETEAEVAAQLAEGRVPIGIGENTKIKDCIIDKNARIGKNVVIANSEGVQEADRSSEGFYIRSGVTVVLKNSTIEDGLVI*