AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0069540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0069540 MtrunA17_Chr1g0200221 93.011 186 9 2 33 214 1 186 3.63e-125 351
Msa0069540 MtrunA17_Chr1g0200231 78.919 185 36 1 33 214 1 185 2.98e-105 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0069540 AT2G35810.2 63.830 188 61 3 26 210 12 195 1.08e-85 252
Msa0069540 AT2G35810.1 63.830 188 61 3 26 210 12 195 1.08e-85 252
Msa0069540 AT2G35820.1 62.903 186 63 2 29 212 1 182 4.13e-84 248
Msa0069540 AT2G35820.2 62.903 186 63 2 29 212 91 272 1.25e-82 247
Msa0069540 AT2G35830.2 64.481 183 63 2 29 210 1 182 1.70e-82 244
Msa0069540 AT2G35830.1 64.324 185 55 4 29 210 1 177 7.40e-78 232

Find 77 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAAACACTCTTGATGATT+TGG 0.216395 1_2:+37979649 None:intergenic
ACGGTTAAGCTTGAGAAATT+TGG 0.260726 1_2:+37980121 None:intergenic
GGTGGTAATGTTTGGTATCT+TGG 0.305200 1_2:-37980278 Msa0069540:CDS
TCATCGGACGGTGAGGGGTT+TGG 0.318746 1_2:-37981446 Msa0069540:CDS
TTGTTTACCTAGCAAATTGT+AGG 0.354027 1_2:+37979329 None:intergenic
AATGAAATCAAAGATGATTC+AGG 0.369755 1_2:-37980227 Msa0069540:CDS
TTCGTGCTTGTTCGTGCATC+AGG 0.379271 1_2:+37979363 None:intergenic
AACTTTCATTATGACAGAAC+AGG 0.395491 1_2:-37979537 Msa0069540:three_prime_UTR
AACCTGAAACCTTGAAAACT+TGG 0.407022 1_2:+37980144 None:intergenic
GGTCCATTGGTGGTAATGTT+TGG 0.421411 1_2:-37980286 Msa0069540:CDS
GAACCCTCAAAAGAAACAAT+TGG 0.430404 1_2:-37979251 Msa0069540:three_prime_UTR
ATATAACATATGTCATTTAA+CGG 0.435830 1_2:+37979741 None:intergenic
GTTGTGATCAAAAGAGTCTC+AGG 0.438258 1_2:+37979281 None:intergenic
TCTCGTGTAGTCCTCTTGTT+TGG 0.443978 1_2:+37979600 None:intergenic
GACCTGACCGTACTCTTTGA+AGG 0.444255 1_2:+37981478 None:intergenic
CCAAATCATCAAGAGTGTTT+CGG 0.449198 1_2:-37979649 Msa0069540:three_prime_UTR
ACTCAGTGCCTTGGGTCCAT+TGG 0.468229 1_2:-37980299 Msa0069540:CDS
AGGTCAAGCTGAGCATCGTT+TGG 0.468746 1_2:+37981422 None:intergenic
CAGTGCCTTGGGTCCATTGG+TGG 0.474720 1_2:-37980296 Msa0069540:CDS
CAAGTGTGACTCAGTGCCTT+GGG 0.479051 1_2:-37980307 Msa0069540:CDS
ATGAAAGTTGTTCATGCAGC+AGG 0.480334 1_2:+37979551 None:intergenic
GCAAGTGTGACTCAGTGCCT+TGG 0.493326 1_2:-37980308 Msa0069540:CDS
TCATTTGAATCAACAATGGA+TGG 0.494781 1_2:+37980245 None:intergenic
TCTATTTAAGTCTGATACAA+TGG 0.496626 1_2:-37980078 Msa0069540:CDS
ACCTTAGCCAAGGAATTCCC+AGG 0.496730 1_2:-37981403 Msa0069540:intron
GAGGCTTCATCGGACGGTGA+GGG 0.503853 1_2:-37981452 Msa0069540:CDS
CTGCACCACGTAATCTGATC+GGG 0.510538 1_2:-37981550 Msa0069540:CDS
ACCTGGGAATTCCTTGGCTA+AGG 0.513339 1_2:+37981402 None:intergenic
AATGGAGAGTGTGAAGGTGA+AGG 0.517827 1_2:-37981522 Msa0069540:CDS
AGTTGTTCATGCAGCAGGCT+AGG 0.523820 1_2:+37979556 None:intergenic
ATGAAAACCTGGGAATTCCT+TGG 0.524061 1_2:+37981396 None:intergenic
TTATCTACCTCCTACCATTG+AGG 0.529148 1_2:-37980171 Msa0069540:CDS
GCAGAATCACTTGAAAGGCT+TGG 0.529417 1_2:+37979773 None:intergenic
TAATCTGATCGGGAAGGGAA+TGG 0.533386 1_2:-37981540 Msa0069540:CDS
CAAACAACCTACAATTTGCT+AGG 0.537329 1_2:-37979336 Msa0069540:three_prime_UTR
GTCATTGAGGCTTCATCGGA+CGG 0.541534 1_2:-37981458 Msa0069540:CDS
GTTCATGCAGCAGGCTAGGA+AGG 0.541928 1_2:+37979560 None:intergenic
AACCGTGGGACATGGCATGC+TGG 0.544561 1_2:-37980104 Msa0069540:CDS
AAAAGAAACAATTGGACCGC+TGG 0.544829 1_2:-37979243 Msa0069540:three_prime_UTR
ATACCAAACATTACCACCAA+TGG 0.547916 1_2:+37980283 None:intergenic
AGGCTTCATCGGACGGTGAG+GGG 0.550679 1_2:-37981451 Msa0069540:CDS
GAAAACTTGGACATCCTCAA+TGG 0.552522 1_2:+37980157 None:intergenic
TCAGGTCATTGAGGCTTCAT+CGG 0.558495 1_2:-37981462 Msa0069540:CDS
TCAAGCTTAACCGTGGGACA+TGG 0.558912 1_2:-37980112 Msa0069540:CDS
GTGATTGTTTAGAATTTGAG+TGG 0.563108 1_2:-37979405 Msa0069540:intron
GATTTCATTTGAATCAACAA+TGG 0.567401 1_2:+37980241 None:intergenic
GACCTTCAAAGAGTACGGTC+AGG 0.570961 1_2:-37981480 Msa0069540:CDS
TGAGGCTTCATCGGACGGTG+AGG 0.573414 1_2:-37981453 Msa0069540:CDS
GCTGCACCACGTAATCTGAT+CGG 0.574183 1_2:-37981551 Msa0069540:CDS
GTCCTTCTTAAAGTTGTGTG+TGG 0.574527 1_2:+37979844 None:intergenic
TACCACACACAACTTTAAGA+AGG 0.575142 1_2:-37979846 Msa0069540:CDS
CCACGTAATCTGATCGGGAA+GGG 0.575321 1_2:-37981545 Msa0069540:CDS
CCGTACTCTTTGAAGGTCGC+CGG 0.579027 1_2:+37981485 None:intergenic
AATGTGCAGAATCACTTGAA+AGG 0.589748 1_2:+37979768 None:intergenic
CACAACTTTAAGAAGGACAA+TGG 0.594981 1_2:-37979839 Msa0069540:CDS
GAAGGGAATGGAGAGTGTGA+AGG 0.597978 1_2:-37981528 Msa0069540:CDS
CAAAGATGATTCAGGCAAGA+CGG 0.600075 1_2:-37980219 Msa0069540:CDS
TAATGTTTGGTATCTTGGAG+TGG 0.602976 1_2:-37980273 Msa0069540:CDS
TGCATTATGTACCAAACAAG+AGG 0.603031 1_2:-37979611 Msa0069540:three_prime_UTR
GACCAGCATGCCATGTCCCA+CGG 0.605299 1_2:+37980102 None:intergenic
CCGGCGACCTTCAAAGAGTA+CGG 0.605471 1_2:-37981485 Msa0069540:CDS
GTGATATTAGAAAACTCAAG+TGG 0.611037 1_2:+37980338 None:intergenic
ACCACGTAATCTGATCGGGA+AGG 0.611825 1_2:-37981546 Msa0069540:CDS
CCCTTCCCGATCAGATTACG+TGG 0.613554 1_2:+37981545 None:intergenic
CTTTAAGAAGGACAATGGAG+TGG 0.614786 1_2:-37979834 Msa0069540:CDS
GAAGGCAATAGAAGCAACTC+CGG 0.616815 1_2:-37981504 Msa0069540:CDS
AAATTTCTCAAGCTTAACCG+TGG 0.618191 1_2:-37980119 Msa0069540:CDS
ACTTGGACATCCTCAATGGT+AGG 0.620998 1_2:+37980161 None:intergenic
CATTACCACCAATGGACCCA+AGG 0.621913 1_2:+37980291 None:intergenic
ATTGTATCAGACTTAAATAG+AGG 0.622516 1_2:+37980080 None:intergenic
GCTCAGCTTGACCTTAGCCA+AGG 0.638940 1_2:-37981413 Msa0069540:CDS
AATTTCTCAAGCTTAACCGT+GGG 0.640376 1_2:-37980118 Msa0069540:CDS
ACTTTCATTATGACAGAACA+GGG 0.644186 1_2:-37979536 Msa0069540:intron
TGGACATCCTCAATGGTAGG+AGG 0.650915 1_2:+37980164 None:intergenic
AGAGTACGGTCAGGTCATTG+AGG 0.671075 1_2:-37981471 Msa0069540:CDS
ACAACAAGACTTGATATACG+TGG 0.723929 1_2:-37981600 Msa0069540:CDS
ACGGTCGTGAAGTCGCACAG+TGG 0.761158 1_2:-37980200 Msa0069540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAAATTCAAAATGAA+TGG + chr1_2:37979495-37979514 None:intergenic 10.0%
!!! AAGTTATTTTGATTAAAAAA+GGG + chr1_2:37979656-37979675 None:intergenic 10.0%
!!! AAAGTTATTTTGATTAAAAA+AGG + chr1_2:37979657-37979676 None:intergenic 10.0%
!!! TTATTTTTTTGGTTTTTTTT+AGG - chr1_2:37980872-37980891 Msa0069540:intron 10.0%
!!! TATTTTTTTGGTTTTTTTTA+GGG - chr1_2:37980873-37980892 Msa0069540:intron 10.0%
!!! TATTTGAAAAGAAATTTTGT+TGG + chr1_2:37979520-37979539 None:intergenic 15.0%
!! AAATTAATTCCAATAAGTTA+GGG + chr1_2:37980404-37980423 None:intergenic 15.0%
!!! ATATAACATATGTCATTTAA+CGG + chr1_2:37981063-37981082 None:intergenic 15.0%
!!! CAAAATTTTCATTTGAATTG+AGG + chr1_2:37979450-37979469 None:intergenic 20.0%
!!! CTCAATTCAAATGAAAATTT+TGG - chr1_2:37979448-37979467 Msa0069540:intron 20.0%
!! TAAATTAAATGACAGCTTTA+TGG + chr1_2:37979564-37979583 None:intergenic 20.0%
!! TAACTTAAGATATTTCGTAA+AGG - chr1_2:37979974-37979993 Msa0069540:intron 20.0%
!!! TTTATTGTTTTATCTGATCT+TGG - chr1_2:37980207-37980226 Msa0069540:CDS 20.0%
!! CAAATTAATTCCAATAAGTT+AGG + chr1_2:37980405-37980424 None:intergenic 20.0%
!!! TTGTGCTTATCTTATTTTTT+TGG - chr1_2:37980861-37980880 Msa0069540:intron 20.0%
!!! ATATCAAGTCTTGTTGTTTT+TGG + chr1_2:37979198-37979217 None:intergenic 25.0%
! ATTTAAGTGATTCTTTGTCA+AGG - chr1_2:37979579-37979598 Msa0069540:three_prime_UTR 25.0%
!!! TCAGTTTTTTATCTGCTATT+TGG - chr1_2:37979617-37979636 Msa0069540:three_prime_UTR 25.0%
! ATTACATTAAACACTGTCAT+TGG + chr1_2:37979945-37979964 None:intergenic 25.0%
! CGAAATATCTTAAGTTATGT+TGG + chr1_2:37979971-37979990 None:intergenic 25.0%
!! ATGATTTTGTATTATGCCTT+TGG + chr1_2:37980081-37980100 None:intergenic 25.0%
! ATTGCAATTACTATTGATAG+TGG + chr1_2:37980155-37980174 None:intergenic 25.0%
! ATAGTAATTGCAATCAACTT+TGG - chr1_2:37980161-37980180 Msa0069540:CDS 25.0%
! CATAATGTCAATCTAAATTG+AGG + chr1_2:37980353-37980372 None:intergenic 25.0%
!!! GGAATTAATTTGTGTTTGTT+AGG - chr1_2:37980413-37980432 Msa0069540:intron 25.0%
! GATTTCATTTGAATCAACAA+TGG + chr1_2:37980563-37980582 None:intergenic 25.0%
! AATGAAATCAAAGATGATTC+AGG - chr1_2:37980574-37980593 Msa0069540:intron 25.0%
! ATTGTATCAGACTTAAATAG+AGG + chr1_2:37980724-37980743 None:intergenic 25.0%
! TCTATTTAAGTCTGATACAA+TGG - chr1_2:37980723-37980742 Msa0069540:intron 25.0%
! ATGCTCTTTACAATAATACA+TGG - chr1_2:37980835-37980854 Msa0069540:intron 25.0%
! TGCTCTTTACAATAATACAT+GGG - chr1_2:37980836-37980855 Msa0069540:intron 25.0%
! ATGTTGTTGTGATTTATGTA+TGG - chr1_2:37980904-37980923 Msa0069540:intron 25.0%
!!! TATGGTTTTTTATTGTGTTC+AGG - chr1_2:37980922-37980941 Msa0069540:intron 25.0%
!! ACTCTATTCAAGTTAGAATA+GGG + chr1_2:37981335-37981354 None:intergenic 25.0%
!! AACTCTATTCAAGTTAGAAT+AGG + chr1_2:37981336-37981355 None:intergenic 25.0%
! AACTTGAATAGAGTTGTTAT+TGG - chr1_2:37981341-37981360 Msa0069540:intron 25.0%
!! ACAAAAACAAAGCATCTTAA+AGG - chr1_2:37981491-37981510 Msa0069540:CDS 25.0%
!! TGTTTTATGATAAATACCAG+CGG + chr1_2:37981577-37981596 None:intergenic 25.0%
! ACATGTTCTTGTTATGCATA+TGG + chr1_2:37979693-37979712 None:intergenic 30.0%
GATGCAAAATATTGATCCAA+AGG - chr1_2:37980062-37980081 Msa0069540:CDS 30.0%
GTTGTTTAAATTCACAGAGA+TGG - chr1_2:37980183-37980202 Msa0069540:CDS 30.0%
TTACATCAGCTTAATTTAGC+TGG - chr1_2:37980312-37980331 Msa0069540:CDS 30.0%
TACATCAGCTTAATTTAGCT+GGG - chr1_2:37980313-37980332 Msa0069540:CDS 30.0%
ATTCCAATAAGTTAGGGTAT+AGG + chr1_2:37980398-37980417 None:intergenic 30.0%
GTGATATTAGAAAACTCAAG+TGG + chr1_2:37980466-37980485 None:intergenic 30.0%
TCATTTGAATCAACAATGGA+TGG + chr1_2:37980559-37980578 None:intergenic 30.0%
CATGTAACACTTTAGTGAAA+GGG + chr1_2:37980797-37980816 None:intergenic 30.0%
!!! TGGTTTTTTTTAGGGTTTAG+TGG - chr1_2:37980881-37980900 Msa0069540:intron 30.0%
AACTTTCATTATGACAGAAC+AGG - chr1_2:37981264-37981283 Msa0069540:intron 30.0%
ACTTTCATTATGACAGAACA+GGG - chr1_2:37981265-37981284 Msa0069540:intron 30.0%
! CTCTATTCAAGTTAGAATAG+GGG + chr1_2:37981334-37981353 None:intergenic 30.0%
! TTGAATAGAGTTGTTATTGG+TGG - chr1_2:37981344-37981363 Msa0069540:intron 30.0%
! GTGATTGTTTAGAATTTGAG+TGG - chr1_2:37981396-37981415 Msa0069540:intron 30.0%
TTGTTTACCTAGCAAATTGT+AGG + chr1_2:37981475-37981494 None:intergenic 30.0%
!!! AGTCTTGTTGTTTTTGGTCA+AGG + chr1_2:37979192-37979211 None:intergenic 35.0%
ACAACAAGACTTGATATACG+TGG - chr1_2:37979201-37979220 Msa0069540:three_prime_UTR 35.0%
GAAGTGAAGAATGAAAACCT+GGG + chr1_2:37979418-37979437 None:intergenic 35.0%
AGAAGTGAAGAATGAAAACC+TGG + chr1_2:37979419-37979438 None:intergenic 35.0%
ATAAAAAACTGAGTGCTTCC+AGG + chr1_2:37979609-37979628 None:intergenic 35.0%
! TCTTGTTATGCATATGGCAT+TGG + chr1_2:37979687-37979706 None:intergenic 35.0%
! GGATTAATCGATTCTTGGAT+TGG - chr1_2:37979891-37979910 Msa0069540:intron 35.0%
AAGTGTAAAGCTTGAATCTG+TGG - chr1_2:37980115-37980134 Msa0069540:CDS 35.0%
CAATAAGTTAGGGTATAGGT+TGG + chr1_2:37980394-37980413 None:intergenic 35.0%
CAACCTATACCCTAACTTAT+TGG - chr1_2:37980392-37980411 Msa0069540:intron 35.0%
ATACCAAACATTACCACCAA+TGG + chr1_2:37980521-37980540 None:intergenic 35.0%
TAATGTTTGGTATCTTGGAG+TGG - chr1_2:37980528-37980547 Msa0069540:intron 35.0%
AACCTGAAACCTTGAAAACT+TGG + chr1_2:37980660-37980679 None:intergenic 35.0%
ACGGTTAAGCTTGAGAAATT+TGG + chr1_2:37980683-37980702 None:intergenic 35.0%
AAATTTCTCAAGCTTAACCG+TGG - chr1_2:37980682-37980701 Msa0069540:intron 35.0%
AATTTCTCAAGCTTAACCGT+GGG - chr1_2:37980683-37980702 Msa0069540:intron 35.0%
TAACACTTTAGTGAAAGGGA+GGG + chr1_2:37980793-37980812 None:intergenic 35.0%
GCATGTAACACTTTAGTGAA+AGG + chr1_2:37980798-37980817 None:intergenic 35.0%
!! AGAGCATTGCAAATGAATTC+AGG + chr1_2:37980822-37980841 None:intergenic 35.0%
!!! GGTTTTTTATTGTGTTCAGG+TGG - chr1_2:37980925-37980944 Msa0069540:intron 35.0%
! TTTTTATTGTGTTCAGGTGG+TGG - chr1_2:37980928-37980947 Msa0069540:intron 35.0%
! TACCACACACAACTTTAAGA+AGG - chr1_2:37980955-37980974 Msa0069540:intron 35.0%
! CACAACTTTAAGAAGGACAA+TGG - chr1_2:37980962-37980981 Msa0069540:intron 35.0%
AATGTGCAGAATCACTTGAA+AGG + chr1_2:37981036-37981055 None:intergenic 35.0%
!! CCAAATCATCAAGAGTGTTT+CGG - chr1_2:37981152-37981171 Msa0069540:intron 35.0%
TGCATTATGTACCAAACAAG+AGG - chr1_2:37981190-37981209 Msa0069540:intron 35.0%
! CAAACAACCTACAATTTGCT+AGG - chr1_2:37981465-37981484 Msa0069540:CDS 35.0%
GAACCCTCAAAAGAAACAAT+TGG - chr1_2:37981550-37981569 Msa0069540:CDS 35.0%
!! GGTCCAATTGTTTCTTTTGA+GGG + chr1_2:37981556-37981575 None:intergenic 35.0%
ATGAAAACCTGGGAATTCCT+TGG + chr1_2:37979408-37979427 None:intergenic 40.0%
TGGAGATCAGTGAGACTATT+TGG + chr1_2:37979475-37979494 None:intergenic 40.0%
! ATGACAGCTTTATGGCGATA+GGG + chr1_2:37979556-37979575 None:intergenic 40.0%
! AATGACAGCTTTATGGCGAT+AGG + chr1_2:37979557-37979576 None:intergenic 40.0%
TTCTTTGACCAAACCCATCT+CGG - chr1_2:37979734-37979753 Msa0069540:three_prime_UTR 40.0%
! CTCTCGGATTAATCGATTCT+TGG - chr1_2:37979886-37979905 Msa0069540:intron 40.0%
! GTCGTAATTTCTCGCTGTTT+TGG - chr1_2:37980232-37980251 Msa0069540:CDS 40.0%
! GGTGGTAATGTTTGGTATCT+TGG - chr1_2:37980523-37980542 Msa0069540:intron 40.0%
CAAAGATGATTCAGGCAAGA+CGG - chr1_2:37980582-37980601 Msa0069540:intron 40.0%
TTATCTACCTCCTACCATTG+AGG - chr1_2:37980630-37980649 Msa0069540:intron 40.0%
GAAAACTTGGACATCCTCAA+TGG + chr1_2:37980647-37980666 None:intergenic 40.0%
! TGAGGATGTCCAAGTTTTCA+AGG - chr1_2:37980648-37980667 Msa0069540:intron 40.0%
!! GTCCAAGTTTTCAAGGTTTC+AGG - chr1_2:37980655-37980674 Msa0069540:intron 40.0%
GTAACACTTTAGTGAAAGGG+AGG + chr1_2:37980794-37980813 None:intergenic 40.0%
GTCCTTCTTAAAGTTGTGTG+TGG + chr1_2:37980960-37980979 None:intergenic 40.0%
! CTTTAAGAAGGACAATGGAG+TGG - chr1_2:37980967-37980986 Msa0069540:intron 40.0%
! CCGAAACACTCTTGATGATT+TGG + chr1_2:37981155-37981174 None:intergenic 40.0%
ATGAAAGTTGTTCATGCAGC+AGG + chr1_2:37981253-37981272 None:intergenic 40.0%
TTCATTATGACAGAACAGGG+TGG - chr1_2:37981268-37981287 Msa0069540:intron 40.0%
TCATTATGACAGAACAGGGT+GGG - chr1_2:37981269-37981288 Msa0069540:intron 40.0%
GTTGTGATCAAAAGAGTCTC+AGG + chr1_2:37981523-37981542 None:intergenic 40.0%
!! CGGTCCAATTGTTTCTTTTG+AGG + chr1_2:37981557-37981576 None:intergenic 40.0%
AAAAGAAACAATTGGACCGC+TGG - chr1_2:37981558-37981577 Msa0069540:CDS 40.0%
TAATCTGATCGGGAAGGGAA+TGG - chr1_2:37979261-37979280 Msa0069540:three_prime_UTR 45.0%
! AATGGAGAGTGTGAAGGTGA+AGG - chr1_2:37979279-37979298 Msa0069540:three_prime_UTR 45.0%
GAAGGCAATAGAAGCAACTC+CGG - chr1_2:37979297-37979316 Msa0069540:three_prime_UTR 45.0%
TCAGGTCATTGAGGCTTCAT+CGG - chr1_2:37979339-37979358 Msa0069540:three_prime_UTR 45.0%
! ATTCTTTGTCAAGGTGCTCC+TGG - chr1_2:37979588-37979607 Msa0069540:three_prime_UTR 45.0%
TCTTTGACCAAACCCATCTC+GGG - chr1_2:37979735-37979754 Msa0069540:three_prime_UTR 45.0%
ATCGAACTTGAGACCTTGAG+AGG + chr1_2:37979786-37979805 None:intergenic 45.0%
! CAAGTTCGATTCGATTCCCT+TGG - chr1_2:37979796-37979815 Msa0069540:exon 45.0%
!! GATTCCCTTGGTGGCAATTT+TGG - chr1_2:37979808-37979827 Msa0069540:exon 45.0%
CTAAGTCCACACAGAGCAAT+GGG - chr1_2:37979835-37979854 Msa0069540:CDS 45.0%
TAAGTCCACACAGAGCAATG+GGG - chr1_2:37979836-37979855 Msa0069540:CDS 45.0%
! GGTCCATTGGTGGTAATGTT+TGG - chr1_2:37980515-37980534 Msa0069540:intron 45.0%
ACTTGGACATCCTCAATGGT+AGG + chr1_2:37980643-37980662 None:intergenic 45.0%
GCAGAATCACTTGAAAGGCT+TGG + chr1_2:37981031-37981050 None:intergenic 45.0%
TCTCGTGTAGTCCTCTTGTT+TGG + chr1_2:37981204-37981223 None:intergenic 45.0%
GCTGCACCACGTAATCTGAT+CGG - chr1_2:37979250-37979269 Msa0069540:three_prime_UTR 50.0%
CTGCACCACGTAATCTGATC+GGG - chr1_2:37979251-37979270 Msa0069540:three_prime_UTR 50.0%
ACCACGTAATCTGATCGGGA+AGG - chr1_2:37979255-37979274 Msa0069540:three_prime_UTR 50.0%
CCACGTAATCTGATCGGGAA+GGG - chr1_2:37979256-37979275 Msa0069540:three_prime_UTR 50.0%
! GAAGGGAATGGAGAGTGTGA+AGG - chr1_2:37979273-37979292 Msa0069540:three_prime_UTR 50.0%
! GACCTTCAAAGAGTACGGTC+AGG - chr1_2:37979321-37979340 Msa0069540:three_prime_UTR 50.0%
GACCTGACCGTACTCTTTGA+AGG + chr1_2:37979326-37979345 None:intergenic 50.0%
AGAGTACGGTCAGGTCATTG+AGG - chr1_2:37979330-37979349 Msa0069540:three_prime_UTR 50.0%
GTCATTGAGGCTTCATCGGA+CGG - chr1_2:37979343-37979362 Msa0069540:three_prime_UTR 50.0%
! AGGTCAAGCTGAGCATCGTT+TGG + chr1_2:37979382-37979401 None:intergenic 50.0%
ACCTTAGCCAAGGAATTCCC+AGG - chr1_2:37979398-37979417 Msa0069540:intron 50.0%
ACCTGGGAATTCCTTGGCTA+AGG + chr1_2:37979402-37979421 None:intergenic 50.0%
! ATCTGAAGCCAACCCGAGAT+GGG + chr1_2:37979750-37979769 None:intergenic 50.0%
! GTTCGATTCGATTCCCTTGG+TGG - chr1_2:37979799-37979818 Msa0069540:exon 50.0%
!! TCCCTTGGTGGCAATTTTGG+TGG - chr1_2:37979811-37979830 Msa0069540:exon 50.0%
CCCACCAAAATTGCCACCAA+GGG + chr1_2:37979815-37979834 None:intergenic 50.0%
!! CCCTTGGTGGCAATTTTGGT+GGG - chr1_2:37979812-37979831 Msa0069540:exon 50.0%
GCTAAGTCCACACAGAGCAA+TGG - chr1_2:37979834-37979853 Msa0069540:CDS 50.0%
TCCGAGAGAACCAATTCCAC+CGG + chr1_2:37979874-37979893 None:intergenic 50.0%
!! GGTATAGGTTGGTGACTAGC+AGG + chr1_2:37980383-37980402 None:intergenic 50.0%
CAAGTGTGACTCAGTGCCTT+GGG - chr1_2:37980494-37980513 Msa0069540:intron 50.0%
CATTACCACCAATGGACCCA+AGG + chr1_2:37980513-37980532 None:intergenic 50.0%
TGGACATCCTCAATGGTAGG+AGG + chr1_2:37980640-37980659 None:intergenic 50.0%
TCAAGCTTAACCGTGGGACA+TGG - chr1_2:37980689-37980708 Msa0069540:intron 50.0%
AGTTGTTCATGCAGCAGGCT+AGG + chr1_2:37981248-37981267 None:intergenic 50.0%
TTCGTGCTTGTTCGTGCATC+AGG + chr1_2:37981441-37981460 None:intergenic 50.0%
CCCTTCCCGATCAGATTACG+TGG + chr1_2:37979259-37979278 None:intergenic 55.0%
! CCGTACTCTTTGAAGGTCGC+CGG + chr1_2:37979319-37979338 None:intergenic 55.0%
CCGGCGACCTTCAAAGAGTA+CGG - chr1_2:37979316-37979335 Msa0069540:three_prime_UTR 55.0%
GCTCAGCTTGACCTTAGCCA+AGG - chr1_2:37979388-37979407 Msa0069540:three_prime_UTR 55.0%
TGACCAAACCCATCTCGGGT+TGG - chr1_2:37979739-37979758 Msa0069540:three_prime_UTR 55.0%
! AAGCCAACCCGAGATGGGTT+TGG + chr1_2:37979745-37979764 None:intergenic 55.0%
! GATCTGAAGCCAACCCGAGA+TGG + chr1_2:37979751-37979770 None:intergenic 55.0%
!! CTCGGGTTGGCTTCAGATCT+TGG - chr1_2:37979752-37979771 Msa0069540:three_prime_UTR 55.0%
CTTGGAGTGTGCTCCTCTCA+AGG - chr1_2:37979770-37979789 Msa0069540:three_prime_UTR 55.0%
GCCCACCAAAATTGCCACCA+AGG + chr1_2:37979816-37979835 None:intergenic 55.0%
! GCCGGTGGAATTGGTTCTCT+CGG - chr1_2:37979870-37979889 Msa0069540:intron 55.0%
GCAAGTGTGACTCAGTGCCT+TGG - chr1_2:37980493-37980512 Msa0069540:intron 55.0%
ACTCAGTGCCTTGGGTCCAT+TGG - chr1_2:37980502-37980521 Msa0069540:intron 55.0%
GTTCATGCAGCAGGCTAGGA+AGG + chr1_2:37981244-37981263 None:intergenic 55.0%
TGAGGCTTCATCGGACGGTG+AGG - chr1_2:37979348-37979367 Msa0069540:three_prime_UTR 60.0%
GAGGCTTCATCGGACGGTGA+GGG - chr1_2:37979349-37979368 Msa0069540:three_prime_UTR 60.0%
AGGCTTCATCGGACGGTGAG+GGG - chr1_2:37979350-37979369 Msa0069540:three_prime_UTR 60.0%
TCATCGGACGGTGAGGGGTT+TGG - chr1_2:37979355-37979374 Msa0069540:three_prime_UTR 60.0%
AATTCCACCGGCCACTTGCG+GGG + chr1_2:37979862-37979881 None:intergenic 60.0%
CAATTCCACCGGCCACTTGC+GGG + chr1_2:37979863-37979882 None:intergenic 60.0%
CCAATTCCACCGGCCACTTG+CGG + chr1_2:37979864-37979883 None:intergenic 60.0%
CAGTGCCTTGGGTCCATTGG+TGG - chr1_2:37980505-37980524 Msa0069540:intron 60.0%
ACGGTCGTGAAGTCGCACAG+TGG - chr1_2:37980601-37980620 Msa0069540:intron 60.0%
AACCGTGGGACATGGCATGC+TGG - chr1_2:37980697-37980716 Msa0069540:intron 60.0%
GACCAGCATGCCATGTCCCA+CGG + chr1_2:37980702-37980721 None:intergenic 60.0%
CCGCAAGTGGCCGGTGGAAT+TGG - chr1_2:37979861-37979880 Msa0069540:intron 65.0%
CGGGGCCCCATTGCTCTGTG+TGG + chr1_2:37979844-37979863 None:intergenic 70.0%
GAGCAATGGGGCCCCGCAAG+TGG - chr1_2:37979848-37979867 Msa0069540:CDS 70.0%
AATGGGGCCCCGCAAGTGGC+CGG - chr1_2:37979852-37979871 Msa0069540:CDS 70.0%
! GGGGCCCCGCAAGTGGCCGG+TGG - chr1_2:37979855-37979874 Msa0069540:CDS 85.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 37979184 37981639 37979184 ID=Msa0069540;Name=Msa0069540
chr1_2 mRNA 37979184 37981639 37979184 ID=Msa0069540-mRNA-1;Parent=Msa0069540;Name=Msa0069540-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|1|1|1|0.66|0.5|4|509|214
chr1_2 exon 37979184 37979415 37979184 ID=Msa0069540-mRNA-1:exon:11991;Parent=Msa0069540-mRNA-1
chr1_2 exon 37979537 37979879 37979537 ID=Msa0069540-mRNA-1:exon:11990;Parent=Msa0069540-mRNA-1
chr1_2 exon 37980046 37980388 37980046 ID=Msa0069540-mRNA-1:exon:11989;Parent=Msa0069540-mRNA-1
chr1_2 exon 37981404 37981639 37981404 ID=Msa0069540-mRNA-1:exon:11988;Parent=Msa0069540-mRNA-1
chr1_2 CDS 37981404 37981639 37981404 ID=Msa0069540-mRNA-1:cds;Parent=Msa0069540-mRNA-1
chr1_2 CDS 37980046 37980388 37980046 ID=Msa0069540-mRNA-1:cds;Parent=Msa0069540-mRNA-1
chr1_2 CDS 37979814 37979879 37979814 ID=Msa0069540-mRNA-1:cds;Parent=Msa0069540-mRNA-1
chr1_2 three_prime_UTR 37979537 37979813 37979537 ID=Msa0069540-mRNA-1:three_prime_utr;Parent=Msa0069540-mRNA-1
chr1_2 three_prime_UTR 37979184 37979415 37979184 ID=Msa0069540-mRNA-1:three_prime_utr;Parent=Msa0069540-mRNA-1
Gene Sequence

>Msa0069540

TACTACCTTGACCAAAAACAACAAGACTTGATATACGTGGCAGAAAGTGATTTTTGTTTTGTTGAAGCTGCACCACGTAATCTGATCGGGAAGGGAATGGAGAGTGTGAAGGTGAAGGCAATAGAAGCAACTCCGGCGACCTTCAAAGAGTACGGTCAGGTCATTGAGGCTTCATCGGACGGTGAGGGGTTTGGTCCAAACGATGCTCAGCTTGACCTTAGCCAAGGAATTCCCAGGTTCTACATTATGCATCTTGAAAATCGTCCACTTGAGTTTTCTAATATCACACATCATGCAAGTGTGACTCAGTGCCTTGGGTCCATTGGTGGTAATGTTTGGTATCTTGGAGTGGCTAAGCCATCCATTGTTGATTCAAATGAAATCAAAGATGATTCAGGCAAGACGGTCGTGAAGTCGCACAGTGGTCATTTTTATCTACCTCCTACCATTGAGGATGTCCAAGTTTTCAAGGTTTCAGGTTCCAAATTTCTCAAGCTTAACCGTGGGACATGGCATGCTGGTCCTCTATTTAAGTCTGATACAATGGACTTTTACAATCTAGAACTGAGCAATACAAATGTGGTGGATCATACCACACACAACTTTAAGAAGGACAATGGAGTGGTTTTTTCGATTGTTGAGTAA
Protein sequence

>Msa0069540

YYLDQKQQDLIYVAESDFCFVEAAPRNLIGKGMESVKVKAIEATPATFKEYGQVIEASSDGEGFGPNDAQLDLSQGIPRFYIMHLENRPLEFSNITHHASVTQCLGSIGGNVWYLGVAKPSIVDSNEIKDDSGKTVVKSHSGHFYLPPTIEDVQVFKVSGSKFLKLNRGTWHAGPLFKSDTMDFYNLELSNTNVVDHTTHNFKKDNGVVFSIVE*