Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0188680 | AAN63591.1 | 99.769 | 433 | 0 | 1 | 1 | 433 | 1 | 432 | 0.0 | 900 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0188680 | sp|Q39019|KSG10_ARATH | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0188680 | 0.874049 | 9.145880e-68 | -8.615850e-47 |
| Msa0000550 | Msa0188680 | 0.845675 | 3.502815e-59 | -8.615850e-47 |
| Msa0001790 | Msa0188680 | -0.832169 | 1.102021e-55 | -8.615850e-47 |
| Msa0001950 | Msa0188680 | -0.815146 | 1.073629e-51 | -8.615850e-47 |
| Msa0003470 | Msa0188680 | -0.808005 | 3.831118e-50 | -8.615850e-47 |
| Msa0004860 | Msa0188680 | -0.823163 | 1.606035e-53 | -8.615850e-47 |
| Msa0004920 | Msa0188680 | -0.834862 | 2.344696e-56 | -8.615850e-47 |
| Msa0008850 | Msa0188680 | -0.814401 | 1.569984e-51 | -8.615850e-47 |
| Msa0011720 | Msa0188680 | -0.854367 | 1.290709e-61 | -8.615850e-47 |
| Msa0012040 | Msa0188680 | -0.815885 | 7.350121e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0188680 | MtrunA17_Chr2g0277011 | 98.614 | 433 | 1 | 2 | 1 | 433 | 1 | 428 | 0.0 | 885 |
| Msa0188680 | MtrunA17_Chr7g0270371 | 72.000 | 425 | 103 | 2 | 6 | 430 | 2 | 410 | 0.0 | 652 |
| Msa0188680 | MtrunA17_Chr1g0193081 | 79.412 | 374 | 77 | 0 | 58 | 431 | 38 | 411 | 0.0 | 648 |
| Msa0188680 | MtrunA17_Chr1g0194201 | 73.892 | 406 | 93 | 1 | 25 | 430 | 18 | 410 | 0.0 | 646 |
| Msa0188680 | MtrunA17_Chr8g0350311 | 68.496 | 419 | 124 | 3 | 5 | 423 | 57 | 467 | 0.0 | 615 |
| Msa0188680 | MtrunA17_Chr2g0320011 | 75.135 | 370 | 91 | 1 | 57 | 426 | 50 | 418 | 0.0 | 613 |
| Msa0188680 | MtrunA17_Chr7g0246591 | 67.883 | 411 | 126 | 1 | 13 | 423 | 65 | 469 | 0.0 | 610 |
| Msa0188680 | MtrunA17_Chr2g0284091 | 70.732 | 369 | 107 | 1 | 57 | 425 | 2 | 369 | 0.0 | 588 |
| Msa0188680 | MtrunA17_Chr4g0045841 | 35.154 | 293 | 173 | 8 | 101 | 384 | 61 | 345 | 4.02e-55 | 187 |
| Msa0188680 | MtrunA17_Chr1g0187371 | 34.828 | 290 | 179 | 5 | 101 | 382 | 24 | 311 | 4.72e-53 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0188680 | AT1G09840.3 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.7 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.10 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.6 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.4 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.1 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.9 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.5 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.8 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
| Msa0188680 | AT1G09840.2 | 84.065 | 433 | 57 | 4 | 1 | 433 | 1 | 421 | 0.0 | 765 |
Find 125 sgRNAs with CRISPR-Local
Find 430 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCTCATCCATGGCACA+AGG | 0.674504 | 2_1:-3885478 | Msa0188680:intron |
| AAATTAAACAGCGGAGGCAA+TGG | 0.491517 | 2_1:+3884203 | None:intergenic |
| AAATTGCAGCCCTTGTTTCC+TGG | 0.344987 | 2_1:-3886023 | Msa0188680:intron |
| AACTTAAATTCAGTATAATT+TGG | 0.096083 | 2_1:+3885510 | None:intergenic |
| AAGCAGTTGATCTTGTCTGT+AGG | 0.606047 | 2_1:-3884434 | Msa0188680:CDS |
| AATCACATAGTTTCAGCTGA+TGG | 0.546019 | 2_1:+3886366 | None:intergenic |
| ACAAGATCAACTGCTTCTGG+AGG | 0.664345 | 2_1:+3884441 | None:intergenic |
| ACAAGCATTGCTGCACGAAA+CGG | 0.523685 | 2_1:-3888758 | Msa0188680:CDS |
| ACAGAATGTTAGTTATATTG+CGG | 0.593159 | 2_1:-3888633 | Msa0188680:intron |
| ACAGCAGTGCATCTCAAATT+AGG | 0.301087 | 2_1:+3884396 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGAATGAAAAGGTAGGT+AGG | + | chr2_1:3883210-3883229 | None:intergenic | 30.0% | |
| ACATAATATAAGAAGAGGAC+AGG | + | chr2_1:3883712-3883731 | None:intergenic | 30.0% | |
| GTGAGGTTGAAAAAGAAAAA+CGG | + | chr2_1:3883737-3883756 | None:intergenic | 30.0% | |
| CTTCCTTGTTAGTTAGATTT+GGG | - | chr2_1:3884196-3884215 | Msa0188680:CDS | 30.0% | |
| ATTCCCAAATCTAACTAACA+AGG | + | chr2_1:3884202-3884221 | None:intergenic | 30.0% | |
| TTGTTAGTTAGATTTGGGAA+TGG | - | chr2_1:3884201-3884220 | Msa0188680:CDS | 30.0% | |
| GCAATTATGTATGTAAGCAA+GGG | + | chr2_1:3884690-3884709 | None:intergenic | 30.0% | |
| TGCAATTATGTATGTAAGCA+AGG | + | chr2_1:3884691-3884710 | None:intergenic | 30.0% | |
| GCTTACATACATAATTGCAT+TGG | - | chr2_1:3884692-3884711 | Msa0188680:intron | 30.0% | |
| TAATAAACAAGCCCATTATG+AGG | + | chr2_1:3884815-3884834 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 3883044 | 3889366 | 3883044 | ID=Msa0188680;Name=Msa0188680 |
| chr2_1 | mRNA | 3883044 | 3889366 | 3883044 | ID=Msa0188680-mRNA-1;Parent=Msa0188680;Name=Msa0188680-mRNA-1;_AED=0.19;_eAED=0.19;_QI=242|1|1|1|0.90|0.83|12|285|433 |
| chr2_1 | exon | 3883044 | 3883417 | 3883044 | ID=Msa0188680-mRNA-1:exon:1665;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3884192 | 3884294 | 3884192 | ID=Msa0188680-mRNA-1:exon:1664;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3884398 | 3884481 | 3884398 | ID=Msa0188680-mRNA-1:exon:1663;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3885479 | 3885574 | 3885479 | ID=Msa0188680-mRNA-1:exon:1662;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3885986 | 3886036 | 3885986 | ID=Msa0188680-mRNA-1:exon:1661;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3886115 | 3886255 | 3886115 | ID=Msa0188680-mRNA-1:exon:1660;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3886346 | 3886402 | 3886346 | ID=Msa0188680-mRNA-1:exon:1659;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3887659 | 3887733 | 3887659 | ID=Msa0188680-mRNA-1:exon:1658;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3888226 | 3888498 | 3888226 | ID=Msa0188680-mRNA-1:exon:1657;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3888592 | 3888651 | 3888592 | ID=Msa0188680-mRNA-1:exon:1656;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3888745 | 3889099 | 3888745 | ID=Msa0188680-mRNA-1:exon:1655;Parent=Msa0188680-mRNA-1 |
| chr2_1 | exon | 3889207 | 3889366 | 3889207 | ID=Msa0188680-mRNA-1:exon:1654;Parent=Msa0188680-mRNA-1 |
| chr2_1 | five_prime_UTR | 3889207 | 3889366 | 3889207 | ID=Msa0188680-mRNA-1:five_prime_utr;Parent=Msa0188680-mRNA-1 |
| chr2_1 | five_prime_UTR | 3889018 | 3889099 | 3889018 | ID=Msa0188680-mRNA-1:five_prime_utr;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3888745 | 3889017 | 3888745 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3888592 | 3888651 | 3888592 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3888226 | 3888498 | 3888226 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3887659 | 3887733 | 3887659 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3886346 | 3886402 | 3886346 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3886115 | 3886255 | 3886115 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3885986 | 3886036 | 3885986 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3885479 | 3885574 | 3885479 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3884398 | 3884481 | 3884398 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3884192 | 3884294 | 3884192 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | CDS | 3883329 | 3883417 | 3883329 | ID=Msa0188680-mRNA-1:cds;Parent=Msa0188680-mRNA-1 |
| chr2_1 | three_prime_UTR | 3883044 | 3883328 | 3883044 | ID=Msa0188680-mRNA-1:three_prime_utr;Parent=Msa0188680-mRNA-1 |
| Gene Sequence |
>Msa0188680 ATGGCGTCTGCTAGCCTTGGAAATGGCGGTATTGGCAGCAGTTCAAGGTCTGCTGCTGCCTTGAGAGCTGCTTCTTCCACTTCTTCCTCCGTCGATTGGCTTGGCAGAGAGATGCTTCACATGAACCTCAATCGTGATCATGACCATGACCATGACGACGACGACGACGATGATGCCAGAGAGAGTGAGCCTGACATCATTGATGGTGTTGGTGCTGAAACTGGACATGTTATACGAACAAGCATTGCTGCACGAAACGGTCAATCTAAGCAGAATGTTAGTTATATTGCGGAACACGTAGTTGGGACAGGCTCTTTCGGAACTGTTTTTCAAGCTAAATGTAGAGAAACAGGAGAGATTGTTGCCATCAAGAAGGTTCTCCAGGACAAGCGTTACAAGAACAGAGAGTTACAGATTATGCAAATGCTCGACCATCCAAATATTGTCGCCCTCAGGCATTGTTTCTTTTCAACCACTGACAAAGAAGAGCTTTACTTGAATCTTGTACTTGAATATGTTCCTGAAACCGTCAATCGTATTGCAAGAAACTATAGCAGGATTAACCAACGAATGCCTTTGATATACGTGAAACTTTATACGTACCAGATATGCAGGGCCCTTGCTTACATACATAATTGCATTGGCATCTGTCATCGTGACATCAAACCTCAGAACCTACTTGTGAACCCGCACACCCATCAGCTGAAACTATGTGATTTTGGGAGTGCAAAAGTGCTGGTGAAAGGAGAACCTAATGTTTCATATATCTGTTCAAGATACTATCGTGCCCCAGAACTTATATTTGGTGCCACTGAATATACAACTGCCATAGATATATGGTCAACTGGTTGTGTAATGGCTGAATTACTTCTTGGCCAGCCCTTGTTTCCTGGAGAGAGTGGAGTTGATCAGCTAGTTGAAATCATTAAGGTTTTGGGAACTCCTACCAGGGAGGAGATTAAGTGCATGAACCCAAATTATACTGAATTTAAGTTTCCGCAGATAAAACCTCATCCATGGCACAAGGTCTTTCAGAAACGCTTGCCTCCAGAAGCAGTTGATCTTGTCTGTAGGTTCTTTCAGTACTCTCCTAATTTGAGATGCACTGCTTTGGAAGCTTGCATTCACCCCTTCTTTGATGAATTAAGGGACCCAAACACCCGCCTTCCCAATGGTCGTCCATTGCCTCCGCTGTTTAATTTTAAACCCCAGGAACTTTCTGGTATTCCACCTGATGTCATCAACCGGCTTATTCCAGAACATGCTCGTAAACAGAATTTATTTATGGCTTTGCACACCTAG |
| Protein sequence |
>Msa0188680 MASASLGNGGIGSSSRSAAALRAASSTSSSVDWLGREMLHMNLNRDHDHDHDDDDDDDARESEPDIIDGVGAETGHVIRTSIAARNGQSKQNVSYIAEHVVGTGSFGTVFQAKCRETGEIVAIKKVLQDKRYKNRELQIMQMLDHPNIVALRHCFFSTTDKEELYLNLVLEYVPETVNRIARNYSRINQRMPLIYVKLYTYQICRALAYIHNCIGICHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVKGEPNVSYICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKPHPWHKVFQKRLPPEAVDLVCRFFQYSPNLRCTALEACIHPFFDELRDPNTRLPNGRPLPPLFNFKPQELSGIPPDVINRLIPEHARKQNLFMALHT* |
