AlfalfaGEDB | Msa1236410

M. sativa cultivar zhongmu-4 / Msa1236410

KEGG: K07904
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0008150 GO:0009504 GO:0009987 GO:0012505 GO:0031410 GO:0031982 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071554 GO:0071840 GO:0097708
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236410	XP_003627536.2	99.569	232	1	0	1	232	1	232	2.98e-167	470

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236410	sp\|Q9LNK1\|RABA3_ARATH	73.077	234	59	3	2	232	5	237	1.87e-117	337

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236410	G7LAV3	99.569	232	1	0	1	232	1	232	1.42e-167	470

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa1236410	0.843939	1.029340e-58	-8.615850e-47
Msa0000190	Msa1236410	0.822251	2.617932e-53	-8.615850e-47
Msa0001940	Msa1236410	0.830491	2.849718e-55	-8.615850e-47
Msa0003700	Msa1236410	0.824829	6.530658e-54	-8.615850e-47
Msa0005890	Msa1236410	0.819892	9.147051e-53	-8.615850e-47
Msa0009180	Msa1236410	0.818658	1.747383e-52	-8.615850e-47
Msa0017510	Msa1236410	0.815491	8.995394e-52	-8.615850e-47
Msa0017950	Msa1236410	0.811728	6.053942e-51	-8.615850e-47
Msa0018000	Msa1236410	0.838252	3.211781e-57	-8.615850e-47
Msa0024550	Msa1236410	0.879601	1.090523e-69	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236410	MtrunA17_Chr8g0346421	99.569	232	1	0	1	232	1	232	2.74e-171	470
Msa1236410	MtrunA17_Chr7g0275991	82.710	214	35	1	21	232	15	228	7.47e-130	365
Msa1236410	MtrunA17_Chr8g0381841	66.986	209	64	2	18	222	9	216	1.95e-97	283
Msa1236410	MtrunA17_Chr3g0123821	64.977	217	68	2	18	230	8	220	1.81e-96	281
Msa1236410	MtrunA17_Chr1g0174281	66.832	202	60	2	18	213	7	207	6.59e-94	274
Msa1236410	MtrunA17_Chr7g0231131	66.500	200	63	1	18	213	7	206	9.85e-92	269
Msa1236410	MtrunA17_Chr2g0283131	58.333	216	88	1	17	232	4	217	4.84e-88	259
Msa1236410	MtrunA17_Chr4g0046261	57.407	216	90	1	17	232	4	217	2.21e-87	257
Msa1236410	MtrunA17_Chr2g0276941	61.692	201	75	1	20	218	9	209	3.16e-86	254
Msa1236410	MtrunA17_Chr4g0040781	56.019	216	93	1	17	232	4	217	1.44e-85	253

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236410	AT1G01200.1	73.077	234	59	3	2	232	5	237	1.90e-118	337
Msa1236410	AT5G65270.1	66.019	206	62	2	18	217	9	212	2.47e-97	283
Msa1236410	AT5G47960.1	67.453	212	63	3	14	220	3	213	1.35e-95	279
Msa1236410	AT3G12160.1	66.667	207	61	2	18	218	7	211	2.18e-95	278
Msa1236410	AT4G39990.1	64.151	212	69	4	22	230	13	220	1.16e-92	271
Msa1236410	AT4G18800.1	58.796	216	82	2	17	231	4	213	2.79e-87	257
Msa1236410	AT5G60860.1	56.744	215	91	1	17	231	4	216	1.68e-86	256
Msa1236410	AT3G15060.1	56.279	215	92	1	17	231	4	216	1.65e-85	253
Msa1236410	AT1G16920.1	57.407	216	87	2	17	231	4	215	4.27e-85	252
Msa1236410	AT1G09630.1	59.204	201	80	1	20	218	6	206	6.19e-85	251

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Find 66 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATAGGCAAGGAAGT+GGG	0.556156	8_2:+15338443	None:intergenic
AAAGAATAGGCAAGGAAGTG+GGG	0.651506	8_2:+15338444	None:intergenic
AAATGTTGGTCTTACCTTAG+GGG	0.622705	8_2:+15338334	None:intergenic
AACTCAACTGCATCTTCAGT+AGG	0.619231	8_2:+15336040	None:intergenic
AAGTAATTAAAGCTCAGATC+TGG	0.376078	8_2:-15337997	Msa1236410:CDS
ACTAGACTCTTATAAAACCC+TGG	0.623421	8_2:+15338264	None:intergenic
ACTAGGACTGTCACTATTAA+TGG	0.266282	8_2:-15338022	Msa1236410:CDS
AGACCAGATGAAATCAATTA+AGG	0.353088	8_2:+15335682	None:intergenic
AGCTCTTACTGGTGTGAATG+TGG	0.537237	8_2:-15335981	Msa1236410:CDS
AGGCCTTAATTGATTTCATC+TGG	0.360304	8_2:-15335685	Msa1236410:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAACATGTTGTGAAACCAA+AGG	-	chr8_2:15335808-15335827	Msa1236410:exon	30.0%
TCTTGTCTTGTGAAGAAAAA+TGG	-	chr8_2:15335863-15335882	Msa1236410:CDS	30.0%
TTGTGAAGAAAAATGGTACA+GGG	-	chr8_2:15335870-15335889	Msa1236410:CDS	30.0%
TTTGACTCAAAGTCAACTAT+TGG	-	chr8_2:15336038-15336057	Msa1236410:CDS	30.0%
AAGTAATTAAAGCTCAGATC+TGG	-	chr8_2:15336099-15336118	Msa1236410:CDS	30.0%
AGTAATTAAAGCTCAGATCT+GGG	-	chr8_2:15336100-15336119	Msa1236410:CDS	30.0%
TGAAACTCAATGTAGTTCAA+GGG	+	chr8_2:15336220-15336239	None:intergenic	30.0%
TTGAAACTCAATGTAGTTCA+AGG	+	chr8_2:15336221-15336240	None:intergenic	30.0%
TTGCAATATTAGTGTGGTAT+TGG	-	chr8_2:15336352-15336371	Msa1236410:intron	30.0%
GTATGTTTCTTACGTCAAAA+TGG	+	chr8_2:15336401-15336420	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	15335629	15338489	15335629	ID=Msa1236410;Name=Msa1236410
chr8_2	mRNA	15335629	15338489	15335629	ID=Msa1236410-mRNA-1;Parent=Msa1236410;Name=Msa1236410-mRNA-1;_AED=0.17;_eAED=0.17;_QI=253\|1\|1\|1\|1\|1\|2\|182\|232
chr8_2	exon	15335629	15336249	15335629	ID=Msa1236410-mRNA-1:exon:5826;Parent=Msa1236410-mRNA-1
chr8_2	exon	15337977	15338489	15337977	ID=Msa1236410-mRNA-1:exon:5825;Parent=Msa1236410-mRNA-1
chr8_2	five_prime_UTR	15338237	15338489	15338237	ID=Msa1236410-mRNA-1:five_prime_utr;Parent=Msa1236410-mRNA-1
chr8_2	CDS	15337977	15338236	15337977	ID=Msa1236410-mRNA-1:cds;Parent=Msa1236410-mRNA-1
chr8_2	CDS	15335811	15336249	15335811	ID=Msa1236410-mRNA-1:cds;Parent=Msa1236410-mRNA-1
chr8_2	three_prime_UTR	15335629	15335810	15335629	ID=Msa1236410-mRNA-1:three_prime_utr;Parent=Msa1236410-mRNA-1

Gene Sequence

>Msa1236410

ATGGTACAGGGTGAGAAGAAGCAAGAAAATGGACACAAGAAAATAGATTATGATATGGAAGAGAAAATAGATTATGTGTTTAAGGTTGTTGTCATTGGTGACTCAGCAGTAGGAAAAACTCAAATATTATCAAGGTTTTCAAAGAATGAGTTTTGCTTTGACTCAAAGTCAACTATTGGAGTTGAATTCCAAACTAGGACTGTCACTATTAATGGTAAAGTAATTAAAGCTCAGATCTGGGATACTGCTGGCCAAGAAAGGTACAGAGCTGTAACAAGTGCATACTACAGAGGTGCATTAGGGGCAATGCTAGTATATGACATAACCAAAAGACAATCATTTGATCATGTTGCTAAATGGGTTGAGGAACTTAGAGCACATGCAGATAACTCAATTGTGATCATGCTAGTTGGTAACAAAGGTGACCTTGTTGATTTGAGAATGGTTCCTACTGAAGATGCAGTTGAGTTTGCAGAAGACCAAGGTTTATTCTTCTCAGAGACTTCAGCTCTTACTGGTGTGAATGTGGAGGGTGCATTTATCAAATTGCTAGAAGAGATTAATAGTGTAGTTTCTAAAAAGGCATTGGAATGTGGTAATGTGAAGGGGATTGCACTAAAAGGTTCTAAGATTGATGTAATTTCAGGTGCTGAATTGGAAATTAGTGAGATAAAGAAGCTGTCTTCATGCTCTTGTTGA

Protein sequence

>Msa1236410

MVQGEKKQENGHKKIDYDMEEKIDYVFKVVVIGDSAVGKTQILSRFSKNEFCFDSKSTIGVEFQTRTVTINGKVIKAQIWDTAGQERYRAVTSAYYRGALGAMLVYDITKRQSFDHVAKWVEELRAHADNSIVIMLVGNKGDLVDLRMVPTEDAVEFAEDQGLFFSETSALTGVNVEGAFIKLLEEINSVVSKKALECGNVKGIALKGSKIDVISGAELEISEIKKLSSCSC*