AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0165550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165550 MtrunA17_Chr1g0153651 95.082 61 3 0 4 64 330 390 1.17e-36 127
Msa0165550 MtrunA17_Chr3g0133821 81.967 61 11 0 4 64 643 703 2.13e-31 114
Msa0165550 MtrunA17_Chr7g0263401 79.032 62 13 0 4 65 678 739 1.21e-28 106
Msa0165550 MtrunA17_Chr1g0181991 75.806 62 15 0 4 65 736 797 7.76e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165550 AT5G42870.3 83.871 62 10 0 4 65 741 802 1.16e-31 115
Msa0165550 AT5G42870.2 83.871 62 10 0 4 65 736 797 1.16e-31 115
Msa0165550 AT5G42870.1 83.871 62 10 0 4 65 741 802 1.16e-31 115
Msa0165550 AT3G09560.3 74.194 62 16 0 4 65 714 775 4.18e-27 102
Msa0165550 AT3G09560.1 74.194 62 16 0 4 65 714 775 4.18e-27 102
Msa0165550 AT3G09560.2 74.194 62 16 0 4 65 714 775 4.18e-27 102
Msa0165550 AT3G09560.4 74.194 62 16 0 4 65 714 775 4.18e-27 102

Find 78 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCAATTCCTGGAATTTC+TGG 0.157366 1_4:+46151437 Msa0165550:three_prime_UTR
CCCTCTTTCTAATAAATTAT+AGG 0.199264 1_4:+46151466 Msa0165550:three_prime_UTR
ATTTCCAAAACCAGCATAAA+AGG 0.243136 1_4:-46151009 None:intergenic
CCTCTTTCTAATAAATTATA+GGG 0.278177 1_4:+46151467 Msa0165550:three_prime_UTR
TCATCATGCAGATTGAATTC+TGG 0.301433 1_4:-46149813 None:intergenic
ATTCAAAATTGCATGTTTAG+AGG 0.306466 1_4:+46150867 Msa0165550:three_prime_UTR
TGCAGGAGGATTTCAATTCC+TGG 0.325571 1_4:+46151428 Msa0165550:intron
TGTGTTGGTGGATTGGTTAT+TGG 0.337464 1_4:+46152415 Msa0165550:three_prime_UTR
TTCCACACATCACAGGAAAA+TGG 0.342813 1_4:+46150400 Msa0165550:intron
TCTAATGTTGAAGCAATATC+AGG 0.344643 1_4:-46152493 None:intergenic
ACTTGTTTAAATATTCTTCA+TGG 0.347003 1_4:-46152622 None:intergenic
CTTTAGCGATTCCAACCTTA+AGG 0.348082 1_4:-46151055 None:intergenic
TATTCACAGCATCAAGCTTC+AGG 0.352336 1_4:-46152523 None:intergenic
CCAGATATATGCCTTTGTTC+TGG 0.354260 1_4:+46149907 Msa0165550:five_prime_UTR
CCAGAACAAAGGCATATATC+TGG 0.367509 1_4:-46149907 None:intergenic
CATCATGCAGATTGAATTCT+GGG 0.380607 1_4:-46149812 None:intergenic
ATATCAGGCAAAATTTATCT+TGG 0.384155 1_4:-46152478 None:intergenic
ATTTATGTATCACCTGGTTA+AGG 0.395576 1_4:-46150492 None:intergenic
GTCAATTCATGCCTTTAGTT+GGG 0.399122 1_4:+46150004 Msa0165550:CDS
TTTAATGCTTCCAGGACATC+AGG 0.400587 1_4:+46150962 Msa0165550:intron
TGATGAAATCAGTTACCTTA+AGG 0.409575 1_4:+46151040 Msa0165550:three_prime_UTR
TTCACCATGTAGTTTATAAA+CGG 0.409709 1_4:+46152591 Msa0165550:three_prime_UTR
GGTCAATTCATGCCTTTAGT+TGG 0.412243 1_4:+46150003 Msa0165550:CDS
AGCATAAAAGGATTTGAATC+AGG 0.417707 1_4:-46150997 None:intergenic
TCTCTTCATGCTCTCGTGAA+TGG 0.419647 1_4:+46151251 Msa0165550:three_prime_UTR
TCACCTGGTTAAGGTTGAAT+AGG 0.421278 1_4:-46150483 None:intergenic
CTGTCCTAGTAGAAATATAT+AGG 0.423588 1_4:+46151544 Msa0165550:three_prime_UTR
CTTAAGGTTGGAATCGCTAA+AGG 0.424789 1_4:+46151056 Msa0165550:three_prime_UTR
TGCCTTTAGTTGGGATTGAC+TGG 0.425638 1_4:+46150013 Msa0165550:CDS
GTTAACTTGTGTTGGTGGAT+TGG 0.431040 1_4:+46152408 Msa0165550:three_prime_UTR
CAGTGCTCGTGCAATTTCTC+AGG 0.436002 1_4:+46150441 Msa0165550:CDS
CACTCATACCCATGAATATC+CGG 0.443608 1_4:-46152653 None:intergenic
CCCTATAATTTATTAGAAAG+AGG 0.449741 1_4:-46151467 None:intergenic
CAGAATGTTGCTCTTGTCAT+GGG 0.450293 1_4:-46152383 None:intergenic
TCAGTTGCACCGGATATTCA+TGG 0.456784 1_4:+46152644 Msa0165550:three_prime_UTR
GGGCAACTTCCAGAAATTCC+AGG 0.456984 1_4:-46151446 None:intergenic
TTTACCTATATATTTCTACT+AGG 0.457876 1_4:-46151548 None:intergenic
AAGGTTGAATAGGAATTGTC+TGG 0.463826 1_4:-46150473 None:intergenic
GAAATTACAACAGGACCATC+TGG 0.470676 1_4:-46150618 None:intergenic
CTTGTTTAAATATTCTTCAT+GGG 0.477673 1_4:-46152621 None:intergenic
GAAATCAGTTACCTTAAGGT+TGG 0.483544 1_4:+46151044 Msa0165550:three_prime_UTR
TAATTGCTCAATCATCTGTC+TGG 0.491049 1_4:+46151500 Msa0165550:three_prime_UTR
CTGATGTTAACTTGTGTTGG+TGG 0.495176 1_4:+46152403 Msa0165550:three_prime_UTR
TGTTGCAGGTCAGACGTTCT+AGG 0.500038 1_4:+46149982 Msa0165550:CDS
CCTATAATTTATTAGAAAGA+GGG 0.509739 1_4:-46151466 None:intergenic
ATTACAAGAATAGAGAATCA+TGG 0.521396 1_4:+46152451 Msa0165550:three_prime_UTR
CCTGTTGTAATTTCCCCTGA+CGG 0.524517 1_4:+46150627 Msa0165550:exon
TCAGAATGTTGCTCTTGTCA+TGG 0.524999 1_4:-46152384 None:intergenic
ACTGCTCAGATGAGTCTGTA+GGG 0.526615 1_4:-46151283 None:intergenic
CCGTCAGGGGAAATTACAAC+AGG 0.531431 1_4:-46150627 None:intergenic
TTTAAACAAGTCAGTTGCAC+CGG 0.536078 1_4:+46152634 Msa0165550:three_prime_UTR
TTTCCTTCTCTATATAGAGA+AGG 0.536561 1_4:+46150654 Msa0165550:three_prime_UTR
AATTCTACAAGTGTACTATC+CGG 0.542398 1_4:-46149846 None:intergenic
ATTCTGATGTTAACTTGTGT+TGG 0.543951 1_4:+46152400 Msa0165550:three_prime_UTR
TAGGAATTGTCTGGTGTGAT+GGG 0.553963 1_4:-46150464 None:intergenic
ATAGGAATTGTCTGGTGTGA+TGG 0.555255 1_4:-46150465 None:intergenic
GACCAGTCAATCCCAACTAA+AGG 0.557857 1_4:-46150015 None:intergenic
GGGATTGACTGGTCACAGAC+AGG 0.560023 1_4:+46150024 Msa0165550:CDS
CTCAATCATCTGTCTGGTGA+AGG 0.560302 1_4:+46151506 Msa0165550:three_prime_UTR
AATCATCTGTCTGGTGAAGG+TGG 0.561005 1_4:+46151509 Msa0165550:three_prime_UTR
AAATATTTATAATTAATCCC+AGG 0.577065 1_4:+46151081 Msa0165550:three_prime_UTR
GACTGCTCAGATGAGTCTGT+AGG 0.590089 1_4:-46151284 None:intergenic
ATACCTTCTCTATATAGAGA+AGG 0.590890 1_4:-46150657 None:intergenic
GCATGATGATAGATGAACTC+CGG 0.599784 1_4:+46149827 Msa0165550:five_prime_UTR
CAGTTGCACCGGATATTCAT+GGG 0.600800 1_4:+46152645 Msa0165550:three_prime_UTR
AGAATTAAGCTCCAGAACAA+AGG 0.602514 1_4:-46149918 None:intergenic
ATTACCGTTTATAAACTACA+TGG 0.606503 1_4:-46152595 None:intergenic
TGCTCAGATGAGTCTGTAGG+GGG 0.614155 1_4:-46151281 None:intergenic
AGAATGTTGCTCTTGTCATG+GGG 0.615248 1_4:-46152382 None:intergenic
ATAAAAGGATTTGAATCAGG+AGG 0.622383 1_4:-46150994 None:intergenic
TTAATGCTTCCAGGACATCA+GGG 0.632684 1_4:+46150963 Msa0165550:intron
ATTCCTATTCAACCTTAACC+AGG 0.638969 1_4:+46150480 Msa0165550:CDS
GGATGGAAAGTCTTACCAGA+TGG 0.639007 1_4:+46150603 Msa0165550:intron
CTGCTCAGATGAGTCTGTAG+GGG 0.650960 1_4:-46151282 None:intergenic
GAATGTTGCTCTTGTCATGG+GGG 0.679917 1_4:-46152381 None:intergenic
TGAATTCGTGAGGAGCTCTG+CGG 0.710130 1_4:-46150849 None:intergenic
ATCATGCAGATTGAATTCTG+GGG 0.722801 1_4:-46149811 None:intergenic
GCTCAGATGAGTCTGTAGGG+GGG 0.751001 1_4:-46151280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTATGAAAAAACATTA+TGG + chr1_4:46151880-46151899 Msa0165550:intron 10.0%
!! AATTTATGAAAAAACATTAT+GGG + chr1_4:46151881-46151900 Msa0165550:intron 10.0%
!! AAAAAAAAAACTAAAACTAA+AGG - chr1_4:46152562-46152581 None:intergenic 10.0%
!! TAATTATTAATTGATGTTCT+AGG + chr1_4:46150277-46150296 Msa0165550:intron 15.0%
!! TATATATAATCAGTATGTTT+AGG - chr1_4:46150764-46150783 None:intergenic 15.0%
!! AAATATTTATAATTAATCCC+AGG + chr1_4:46151081-46151100 Msa0165550:three_prime_UTR 15.0%
!! AAGAAATATTTAGATACTAA+TGG + chr1_4:46151843-46151862 Msa0165550:intron 15.0%
!! ATTTATGAAAAAACATTATG+GGG + chr1_4:46151882-46151901 Msa0165550:intron 15.0%
!! AAATACATAACATTAGATAA+TGG - chr1_4:46152278-46152297 None:intergenic 15.0%
!! TAATGTTATGTATTTATTGT+TGG + chr1_4:46152283-46152302 Msa0165550:intron 15.0%
!! ATATATCTGGATGTTAAAAA+TGG - chr1_4:46149897-46149916 None:intergenic 20.0%
!!! TCATTTATTTTCAGAAATCA+AGG + chr1_4:46150053-46150072 Msa0165550:CDS 20.0%
!! AAAACATAACTACTATAGAA+GGG - chr1_4:46150722-46150741 None:intergenic 20.0%
!! AAAAACATAACTACTATAGA+AGG - chr1_4:46150723-46150742 None:intergenic 20.0%
!! CCTATAATTTATTAGAAAGA+GGG - chr1_4:46151469-46151488 None:intergenic 20.0%
!! CCTCTTTCTAATAAATTATA+GGG + chr1_4:46151467-46151486 Msa0165550:three_prime_UTR 20.0%
!!! TTTACCTATATATTTCTACT+AGG - chr1_4:46151551-46151570 None:intergenic 20.0%
!!! AAATGCCTGATAATTTTTAA+TGG + chr1_4:46151619-46151638 Msa0165550:intron 20.0%
!!! AATGCCTGATAATTTTTAAT+GGG + chr1_4:46151620-46151639 Msa0165550:intron 20.0%
!! AAAACCCATTAAAAATTATC+AGG - chr1_4:46151627-46151646 None:intergenic 20.0%
!! TACTTGAAAATAATCTTTGA+TGG - chr1_4:46152029-46152048 None:intergenic 20.0%
!!! CAAAGATTATTTTCAAGTAT+TGG + chr1_4:46152030-46152049 Msa0165550:intron 20.0%
!! CTTGTTTAAATATTCTTCAT+GGG - chr1_4:46152624-46152643 None:intergenic 20.0%
!! ACTTGTTTAAATATTCTTCA+TGG - chr1_4:46152625-46152644 None:intergenic 20.0%
! ATTGTCATAATCAATCATGT+GGG + chr1_4:46150160-46150179 Msa0165550:intron 25.0%
!!! ATCAGTTGTTTTATCTGTTA+AGG + chr1_4:46150213-46150232 Msa0165550:intron 25.0%
! AATAAAAACCAGAAGATTGT+TGG - chr1_4:46150340-46150359 None:intergenic 25.0%
! ATTTATCTTGTGCGTATTTA+TGG + chr1_4:46150361-46150380 Msa0165550:intron 25.0%
! TTTCAAATTTATGTATCACC+TGG - chr1_4:46150501-46150520 None:intergenic 25.0%
! AAACATAACTACTATAGAAG+GGG - chr1_4:46150721-46150740 None:intergenic 25.0%
! ATTCAAAATTGCATGTTTAG+AGG + chr1_4:46150867-46150886 Msa0165550:three_prime_UTR 25.0%
! CCCTCTTTCTAATAAATTAT+AGG + chr1_4:46151466-46151485 Msa0165550:three_prime_UTR 25.0%
! CCCTATAATTTATTAGAAAG+AGG - chr1_4:46151470-46151489 None:intergenic 25.0%
! TCTATACTCTGATTAATCAA+AGG - chr1_4:46151653-46151672 None:intergenic 25.0%
! TTTCCTACAAAAATCCTAAA+GGG - chr1_4:46151697-46151716 None:intergenic 25.0%
!! AACCTTTTGTGTAAAAAACA+GGG + chr1_4:46152138-46152157 Msa0165550:intron 25.0%
! TGATTAAACATTGTTCATAC+AGG + chr1_4:46152345-46152364 Msa0165550:intron 25.0%
! ATTACAAGAATAGAGAATCA+TGG + chr1_4:46152451-46152470 Msa0165550:three_prime_UTR 25.0%
! ATATCAGGCAAAATTTATCT+TGG - chr1_4:46152481-46152500 None:intergenic 25.0%
! TTCACCATGTAGTTTATAAA+CGG + chr1_4:46152591-46152610 Msa0165550:three_prime_UTR 25.0%
! ATTACCGTTTATAAACTACA+TGG - chr1_4:46152598-46152617 None:intergenic 25.0%
!! ATCTCATCAATAGATTTTTG+TGG - chr1_4:46152680-46152699 None:intergenic 25.0%
AATTCTACAAGTGTACTATC+CGG - chr1_4:46149849-46149868 None:intergenic 30.0%
!!! TCTCTTTTTTTATGTGTTGC+AGG + chr1_4:46149968-46149987 Msa0165550:exon 30.0%
CATTGTCATAATCAATCATG+TGG + chr1_4:46150159-46150178 Msa0165550:intron 30.0%
ATTTATGTATCACCTGGTTA+AGG - chr1_4:46150495-46150514 None:intergenic 30.0%
CAAAACAAGAAGACAAAAGA+AGG + chr1_4:46150544-46150563 Msa0165550:intron 30.0%
TGCATCATAACAAAACAAAC+AGG - chr1_4:46150583-46150602 None:intergenic 30.0%
!!! TGTTTGTTTTGTTATGATGC+AGG + chr1_4:46150582-46150601 Msa0165550:intron 30.0%
TTTCCTTCTCTATATAGAGA+AGG + chr1_4:46150654-46150673 Msa0165550:three_prime_UTR 30.0%
ATACCTTCTCTATATAGAGA+AGG - chr1_4:46150660-46150679 None:intergenic 30.0%
AAATTGCATGTTTAGAGGTT+TGG + chr1_4:46150872-46150891 Msa0165550:intron 30.0%
AAGTTGAATTTAATGCTTCC+AGG + chr1_4:46150954-46150973 Msa0165550:intron 30.0%
ATAAAAGGATTTGAATCAGG+AGG - chr1_4:46150997-46151016 None:intergenic 30.0%
AGCATAAAAGGATTTGAATC+AGG - chr1_4:46151000-46151019 None:intergenic 30.0%
! TGATTCAAATCCTTTTATGC+TGG + chr1_4:46150999-46151018 Msa0165550:three_prime_UTR 30.0%
!!! AAATCCTTTTATGCTGGTTT+TGG + chr1_4:46151005-46151024 Msa0165550:three_prime_UTR 30.0%
ATTTCCAAAACCAGCATAAA+AGG - chr1_4:46151012-46151031 None:intergenic 30.0%
TGATGAAATCAGTTACCTTA+AGG + chr1_4:46151040-46151059 Msa0165550:three_prime_UTR 30.0%
ATTTCAATTCCTGGAATTTC+TGG + chr1_4:46151437-46151456 Msa0165550:three_prime_UTR 30.0%
CTGTCCTAGTAGAAATATAT+AGG + chr1_4:46151544-46151563 Msa0165550:three_prime_UTR 30.0%
ATTTAATAACTCCTATGCTG+GGG - chr1_4:46151603-46151622 None:intergenic 30.0%
CATTTAATAACTCCTATGCT+GGG - chr1_4:46151604-46151623 None:intergenic 30.0%
CTTTCCTACAAAAATCCTAA+AGG - chr1_4:46151698-46151717 None:intergenic 30.0%
ATATTTAGATACTAATGGCG+TGG + chr1_4:46151848-46151867 Msa0165550:intron 30.0%
! CAACCTTTTGTGTAAAAAAC+AGG + chr1_4:46152137-46152156 Msa0165550:intron 30.0%
!! ATTCTGATGTTAACTTGTGT+TGG + chr1_4:46152400-46152419 Msa0165550:three_prime_UTR 30.0%
TCTAATGTTGAAGCAATATC+AGG - chr1_4:46152496-46152515 None:intergenic 30.0%
TCATCATGCAGATTGAATTC+TGG - chr1_4:46149816-46149835 None:intergenic 35.0%
TGGATGTTAAAAATGGTTGC+AGG - chr1_4:46149890-46149909 None:intergenic 35.0%
AGAATTAAGCTCCAGAACAA+AGG - chr1_4:46149921-46149940 None:intergenic 35.0%
GTCAATTCATGCCTTTAGTT+GGG + chr1_4:46150004-46150023 Msa0165550:CDS 35.0%
AAGGTTGAATAGGAATTGTC+TGG - chr1_4:46150476-46150495 None:intergenic 35.0%
ATTCCTATTCAACCTTAACC+AGG + chr1_4:46150480-46150499 Msa0165550:CDS 35.0%
!! TGTTTTGTTATGATGCAGGA+TGG + chr1_4:46150586-46150605 Msa0165550:intron 35.0%
! CATGCAATTTTGAATTCGTG+AGG - chr1_4:46150862-46150881 None:intergenic 35.0%
! ATTTGAGTATCGTCATTGCT+AGG + chr1_4:46150926-46150945 Msa0165550:intron 35.0%
GAAATCAGTTACCTTAAGGT+TGG + chr1_4:46151044-46151063 Msa0165550:three_prime_UTR 35.0%
TAATGAGAGACTCATTACCT+GGG - chr1_4:46151101-46151120 None:intergenic 35.0%
ATAATGAGAGACTCATTACC+TGG - chr1_4:46151102-46151121 None:intergenic 35.0%
ACTACATGAGAATGGAAGTT+TGG - chr1_4:46151166-46151185 None:intergenic 35.0%
TAGACATGACTACATGAGAA+TGG - chr1_4:46151174-46151193 None:intergenic 35.0%
TTCTCATGTAGTCATGTCTA+AGG + chr1_4:46151174-46151193 Msa0165550:intron 35.0%
! TAGTCATGTCTAAGGTTTCA+GGG + chr1_4:46151182-46151201 Msa0165550:intron 35.0%
! ATTTTGTGTCTAAGCAACGT+CGG - chr1_4:46151223-46151242 None:intergenic 35.0%
CCTTGTGACGTCATATATAT+GGG - chr1_4:46151367-46151386 None:intergenic 35.0%
TCCTTGTGACGTCATATATA+TGG - chr1_4:46151368-46151387 None:intergenic 35.0%
TAATTGCTCAATCATCTGTC+TGG + chr1_4:46151500-46151519 Msa0165550:three_prime_UTR 35.0%
GCATTTAATAACTCCTATGC+TGG - chr1_4:46151605-46151624 None:intergenic 35.0%
! TTAGGACTTTTCTTCCCTTT+AGG + chr1_4:46151680-46151699 Msa0165550:intron 35.0%
!!! CTTCCCTTTAGGATTTTTGT+AGG + chr1_4:46151691-46151710 Msa0165550:intron 35.0%
TATCTATACTCAAACCACGT+AGG - chr1_4:46151736-46151755 None:intergenic 35.0%
AGCCTTCCTTTGTAGAAATT+GGG - chr1_4:46151800-46151819 None:intergenic 35.0%
TAGCCTTCCTTTGTAGAAAT+TGG - chr1_4:46151801-46151820 None:intergenic 35.0%
GAAGGCTAAAGACAAACTAT+AGG + chr1_4:46151813-46151832 Msa0165550:intron 35.0%
! TTGCTGTTGATATGTCTGTT+TGG + chr1_4:46151951-46151970 Msa0165550:intron 35.0%
! TATTGGTCTCAATGTCTTGA+GGG + chr1_4:46152047-46152066 Msa0165550:intron 35.0%
!! AAAGTGTTTTGTCATGTGAC+TGG + chr1_4:46152089-46152108 Msa0165550:intron 35.0%
!!! TGCCCTGTTTTTTACACAAA+AGG - chr1_4:46152143-46152162 None:intergenic 35.0%
TTTGTGTAAAAAACAGGGCA+AGG + chr1_4:46152143-46152162 Msa0165550:intron 35.0%
CGTACAATACACAAAATGGT+GGG + chr1_4:46152168-46152187 Msa0165550:intron 35.0%
TCCAAGACATTGAGACTAAA+AGG - chr1_4:46152242-46152261 None:intergenic 35.0%
! CATTAGATAATGGTAATGCG+AGG - chr1_4:46152268-46152287 None:intergenic 35.0%
TTTAAACAAGTCAGTTGCAC+CGG + chr1_4:46152634-46152653 Msa0165550:three_prime_UTR 35.0%
GCATGATGATAGATGAACTC+CGG + chr1_4:46149827-46149846 Msa0165550:five_prime_UTR 40.0%
! CCAGAACAAAGGCATATATC+TGG - chr1_4:46149910-46149929 None:intergenic 40.0%
CCAGATATATGCCTTTGTTC+TGG + chr1_4:46149907-46149926 Msa0165550:five_prime_UTR 40.0%
GGTCAATTCATGCCTTTAGT+TGG + chr1_4:46150003-46150022 Msa0165550:CDS 40.0%
ATGTGGGTTCTTAAAACACG+TGG + chr1_4:46150176-46150195 Msa0165550:intron 40.0%
AACCAGAAGATTGTTGGTGT+GGG - chr1_4:46150334-46150353 None:intergenic 40.0%
AAACCAGAAGATTGTTGGTG+TGG - chr1_4:46150335-46150354 None:intergenic 40.0%
TTTCCTGTGATGTGTGGAAT+AGG - chr1_4:46150399-46150418 None:intergenic 40.0%
TTCCACACATCACAGGAAAA+TGG + chr1_4:46150400-46150419 Msa0165550:intron 40.0%
!! GAGCACTGAGAAAAAGTATC+TGG - chr1_4:46150429-46150448 None:intergenic 40.0%
TAGGAATTGTCTGGTGTGAT+GGG - chr1_4:46150467-46150486 None:intergenic 40.0%
ATAGGAATTGTCTGGTGTGA+TGG - chr1_4:46150468-46150487 None:intergenic 40.0%
TCACCTGGTTAAGGTTGAAT+AGG - chr1_4:46150486-46150505 None:intergenic 40.0%
GAAATTACAACAGGACCATC+TGG - chr1_4:46150621-46150640 None:intergenic 40.0%
AGAAGGAAAAAATCCGTCAG+GGG - chr1_4:46150643-46150662 None:intergenic 40.0%
GAGAAGGAAAAAATCCGTCA+GGG - chr1_4:46150644-46150663 None:intergenic 40.0%
AGAGAAGGAAAAAATCCGTC+AGG - chr1_4:46150645-46150664 None:intergenic 40.0%
TTTAATGCTTCCAGGACATC+AGG + chr1_4:46150962-46150981 Msa0165550:intron 40.0%
TTAATGCTTCCAGGACATCA+GGG + chr1_4:46150963-46150982 Msa0165550:intron 40.0%
CTTTAGCGATTCCAACCTTA+AGG - chr1_4:46151058-46151077 None:intergenic 40.0%
CTTAAGGTTGGAATCGCTAA+AGG + chr1_4:46151056-46151075 Msa0165550:three_prime_UTR 40.0%
! GTAGTCATGTCTAAGGTTTC+AGG + chr1_4:46151181-46151200 Msa0165550:intron 40.0%
CCCATATATATGACGTCACA+AGG + chr1_4:46151364-46151383 Msa0165550:intron 40.0%
CAGAGTATAGAAGCTCTGTT+AGG + chr1_4:46151662-46151681 Msa0165550:intron 40.0%
TGATTGAATCTAGACCTACG+TGG + chr1_4:46151719-46151738 Msa0165550:intron 40.0%
CGCTCTATTTGATCTACTCT+CGG - chr1_4:46151774-46151793 None:intergenic 40.0%
AGCCCAATTTCTACAAAGGA+AGG + chr1_4:46151795-46151814 Msa0165550:intron 40.0%
ACCATCTGACCTTACTTAGT+AGG + chr1_4:46151917-46151936 Msa0165550:intron 40.0%
CCATCTGACCTTACTTAGTA+GGG + chr1_4:46151918-46151937 Msa0165550:intron 40.0%
TGACCTTACTTAGTAGGGTA+AGG + chr1_4:46151923-46151942 Msa0165550:intron 40.0%
ATGCCTTACCCTACTAAGTA+AGG - chr1_4:46151929-46151948 None:intergenic 40.0%
! GTATTGGTCTCAATGTCTTG+AGG + chr1_4:46152046-46152065 Msa0165550:intron 40.0%
! ATTGGTCTCAATGTCTTGAG+GGG + chr1_4:46152048-46152067 Msa0165550:intron 40.0%
! TGTTTTGTCATGTGACTGGA+AGG + chr1_4:46152093-46152112 Msa0165550:intron 40.0%
GGTGCGTACAATACACAAAA+TGG + chr1_4:46152164-46152183 Msa0165550:intron 40.0%
GCGTACAATACACAAAATGG+TGG + chr1_4:46152167-46152186 Msa0165550:intron 40.0%
! GCCTTTTAGTCTCAATGTCT+TGG + chr1_4:46152238-46152257 Msa0165550:intron 40.0%
AGAATGTTGCTCTTGTCATG+GGG - chr1_4:46152385-46152404 None:intergenic 40.0%
CAGAATGTTGCTCTTGTCAT+GGG - chr1_4:46152386-46152405 None:intergenic 40.0%
TCAGAATGTTGCTCTTGTCA+TGG - chr1_4:46152387-46152406 None:intergenic 40.0%
!! CTGATGTTAACTTGTGTTGG+TGG + chr1_4:46152403-46152422 Msa0165550:three_prime_UTR 40.0%
!! GTTAACTTGTGTTGGTGGAT+TGG + chr1_4:46152408-46152427 Msa0165550:three_prime_UTR 40.0%
!! TGTGTTGGTGGATTGGTTAT+TGG + chr1_4:46152415-46152434 Msa0165550:three_prime_UTR 40.0%
TATTCACAGCATCAAGCTTC+AGG - chr1_4:46152526-46152545 None:intergenic 40.0%
CACTCATACCCATGAATATC+CGG - chr1_4:46152656-46152675 None:intergenic 40.0%
TGCCTTTAGTTGGGATTGAC+TGG + chr1_4:46150013-46150032 Msa0165550:CDS 45.0%
GACCAGTCAATCCCAACTAA+AGG - chr1_4:46150018-46150037 None:intergenic 45.0%
TGTGATGTGTGGAATAGGCA+AGG - chr1_4:46150394-46150413 None:intergenic 45.0%
TTGCCTATTCCACACATCAC+AGG + chr1_4:46150393-46150412 Msa0165550:intron 45.0%
!! AGCCATTTTCCTGTGATGTG+TGG - chr1_4:46150405-46150424 None:intergenic 45.0%
GGATGGAAAGTCTTACCAGA+TGG + chr1_4:46150603-46150622 Msa0165550:intron 45.0%
CCTGTTGTAATTTCCCCTGA+CGG + chr1_4:46150627-46150646 Msa0165550:exon 45.0%
ACTATAGAAGGGGAAGAGTG+AGG - chr1_4:46150711-46150730 None:intergenic 45.0%
TCTCTTCATGCTCTCGTGAA+TGG + chr1_4:46151251-46151270 Msa0165550:three_prime_UTR 45.0%
ACTGCTCAGATGAGTCTGTA+GGG - chr1_4:46151286-46151305 None:intergenic 45.0%
CCAATGTTCAGCAAGCACAA+GGG - chr1_4:46151407-46151426 None:intergenic 45.0%
ACCAATGTTCAGCAAGCACA+AGG - chr1_4:46151408-46151427 None:intergenic 45.0%
TGCAGGAGGATTTCAATTCC+TGG + chr1_4:46151428-46151447 Msa0165550:intron 45.0%
CTCAATCATCTGTCTGGTGA+AGG + chr1_4:46151506-46151525 Msa0165550:three_prime_UTR 45.0%
AATCATCTGTCTGGTGAAGG+TGG + chr1_4:46151509-46151528 Msa0165550:three_prime_UTR 45.0%
ATATGTCTAGACCCCAGCAT+AGG + chr1_4:46151589-46151608 Msa0165550:intron 45.0%
CCCTACTAAGTAAGGTCAGA+TGG - chr1_4:46151921-46151940 None:intergenic 45.0%
AATGTCTTGAGGGGTAACCT+TGG + chr1_4:46152057-46152076 Msa0165550:intron 45.0%
AACACTTTACAGTTGCGCCA+AGG - chr1_4:46152077-46152096 None:intergenic 45.0%
!! ATTGAGACTAAAAGGCACCC+AGG - chr1_4:46152234-46152253 None:intergenic 45.0%
GAATGTTGCTCTTGTCATGG+GGG - chr1_4:46152384-46152403 None:intergenic 45.0%
TCAGTTGCACCGGATATTCA+TGG + chr1_4:46152644-46152663 Msa0165550:three_prime_UTR 45.0%
CAGTTGCACCGGATATTCAT+GGG + chr1_4:46152645-46152664 Msa0165550:three_prime_UTR 45.0%
TGTTGCAGGTCAGACGTTCT+AGG + chr1_4:46149982-46150001 Msa0165550:CDS 50.0%
GAAGATTGTTGGTGTGGGCA+GGG - chr1_4:46150329-46150348 None:intergenic 50.0%
AGAAGATTGTTGGTGTGGGC+AGG - chr1_4:46150330-46150349 None:intergenic 50.0%
TGCCCACACCAACAATCTTC+TGG + chr1_4:46150329-46150348 Msa0165550:intron 50.0%
CAGTGCTCGTGCAATTTCTC+AGG + chr1_4:46150441-46150460 Msa0165550:CDS 50.0%
CCGTCAGGGGAAATTACAAC+AGG - chr1_4:46150630-46150649 None:intergenic 50.0%
TGAATTCGTGAGGAGCTCTG+CGG - chr1_4:46150852-46150871 None:intergenic 50.0%
!! GAAAAAGTGCCCTGATGTCC+TGG - chr1_4:46150975-46150994 None:intergenic 50.0%
TGCTCAGATGAGTCTGTAGG+GGG - chr1_4:46151284-46151303 None:intergenic 50.0%
CTGCTCAGATGAGTCTGTAG+GGG - chr1_4:46151285-46151304 None:intergenic 50.0%
GACTGCTCAGATGAGTCTGT+AGG - chr1_4:46151287-46151306 None:intergenic 50.0%
! CCCTTGTGCTTGCTGAACAT+TGG + chr1_4:46151404-46151423 Msa0165550:intron 50.0%
! GCTTGCTGAACATTGGTTGC+AGG + chr1_4:46151411-46151430 Msa0165550:intron 50.0%
! TGCTGAACATTGGTTGCAGG+AGG + chr1_4:46151414-46151433 Msa0165550:intron 50.0%
GGGCAACTTCCAGAAATTCC+AGG - chr1_4:46151449-46151468 None:intergenic 50.0%
GCGCAGCCCAATTTCTACAA+AGG + chr1_4:46151791-46151810 Msa0165550:intron 50.0%
GTCATGTGACTGGAAGGTCA+CGG + chr1_4:46152099-46152118 Msa0165550:intron 50.0%
TCATGTGACTGGAAGGTCAC+GGG + chr1_4:46152100-46152119 Msa0165550:intron 50.0%
! GGGATTGACTGGTCACAGAC+AGG + chr1_4:46150024-46150043 Msa0165550:CDS 55.0%
TGTTGGTGTGGGCAGGGTAA+AGG - chr1_4:46150323-46150342 None:intergenic 55.0%
GCTCAGATGAGTCTGTAGGG+GGG - chr1_4:46151283-46151302 None:intergenic 55.0%
GAAGGTCACGGGTTCAAGTC+TGG + chr1_4:46152111-46152130 Msa0165550:intron 55.0%
! AAAATGGTGGGACCCCTTCC+CGG + chr1_4:46152180-46152199 Msa0165550:intron 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr1_4:46152207-46152226 None:intergenic 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr1_4:46152208-46152227 None:intergenic 55.0%
! TGCGGGAGCTTTAGTGCACC+TGG + chr1_4:46152213-46152232 Msa0165550:intron 60.0%
! GCGGGAGCTTTAGTGCACCT+GGG + chr1_4:46152214-46152233 Msa0165550:intron 60.0%
CATACGCAGGGTCCGGGAAG+GGG - chr1_4:46152195-46152214 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG - chr1_4:46152196-46152215 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG + chr1_4:46152195-46152214 Msa0165550:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG + chr1_4:46152196-46152215 Msa0165550:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG - chr1_4:46152197-46152216 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG - chr1_4:46152201-46152220 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG - chr1_4:46152202-46152221 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 46149813 46152704 46149813 ID=Msa0165550;Name=Msa0165550
chr1_4 mRNA 46149813 46152704 46149813 ID=Msa0165550-mRNA-1;Parent=Msa0165550;Name=Msa0165550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=166|1|1|1|0.28|0.25|8|783|71
chr1_4 exon 46149813 46150074 46149813 ID=Msa0165550-mRNA-1:exon:13216;Parent=Msa0165550-mRNA-1
chr1_4 exon 46150415 46150501 46150415 ID=Msa0165550-mRNA-1:exon:13217;Parent=Msa0165550-mRNA-1
chr1_4 exon 46150604 46150675 46150604 ID=Msa0165550-mRNA-1:exon:13218;Parent=Msa0165550-mRNA-1
chr1_4 exon 46150845 46150888 46150845 ID=Msa0165550-mRNA-1:exon:13219;Parent=Msa0165550-mRNA-1
chr1_4 exon 46150976 46151102 46150976 ID=Msa0165550-mRNA-1:exon:13220;Parent=Msa0165550-mRNA-1
chr1_4 exon 46151203 46151304 46151203 ID=Msa0165550-mRNA-1:exon:13221;Parent=Msa0165550-mRNA-1
chr1_4 exon 46151433 46151565 46151433 ID=Msa0165550-mRNA-1:exon:13222;Parent=Msa0165550-mRNA-1
chr1_4 exon 46152367 46152704 46152367 ID=Msa0165550-mRNA-1:exon:13223;Parent=Msa0165550-mRNA-1
chr1_4 five_prime_UTR 46149813 46149978 46149813 ID=Msa0165550-mRNA-1:five_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 CDS 46149979 46150074 46149979 ID=Msa0165550-mRNA-1:cds;Parent=Msa0165550-mRNA-1
chr1_4 CDS 46150415 46150501 46150415 ID=Msa0165550-mRNA-1:cds;Parent=Msa0165550-mRNA-1
chr1_4 CDS 46150604 46150636 46150604 ID=Msa0165550-mRNA-1:cds;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46150637 46150675 46150637 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46150845 46150888 46150845 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46150976 46151102 46150976 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46151203 46151304 46151203 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46151433 46151565 46151433 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
chr1_4 three_prime_UTR 46152367 46152704 46152367 ID=Msa0165550-mRNA-1:three_prime_utr;Parent=Msa0165550-mRNA-1
Gene Sequence

>Msa0165550

ATGTGTTGCAGGTCAGACGTTCTAGGTCAATTCATGCCTTTAGTTGGGATTGACTGGTCACAGACAGGAGTTGCTCATTTATTTTCAGAAATCAAGGAAAATGGCTACCAGATACTTTTTCTCAGTGCTCGTGCAATTTCTCAGGCCCATCACACCAGACAATTCCTATTCAACCTTAACCAGGATGGAAAGTCTTACCAGATGGTCCTGTTGTAA
Protein sequence

>Msa0165550

MCCRSDVLGQFMPLVGIDWSQTGVAHLFSEIKENGYQILFLSARAISQAHHTRQFLFNLNQDGKSYQMVLL*