AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1224200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224200 MtrunA17_Chr4g0076471 74.091 220 27 5 1 193 7 223 5.81e-101 294
Msa1224200 MtrunA17_Chr4g0076451 37.405 131 50 5 93 193 50 178 1.02e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224200 AT5G47740.2 37.821 156 63 5 63 192 21 168 4.80e-22 90.1
Msa1224200 AT5G47740.1 37.662 154 64 5 63 192 21 166 1.04e-21 89.4

Find 40 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTGTTTGATTACAATTT+CGG 0.312597 8_1:-88998383 Msa1224200:CDS
GAAACTAAATATAAGGAAAT+TGG 0.325459 8_1:-88997402 Msa1224200:CDS
GAACTACCCAATCTAATCTA+AGG 0.351629 8_1:-88997373 None:intergenic
TTTGAAGAAGGAAAGTAGAT+TGG 0.355768 8_1:-88998318 Msa1224200:CDS
TTGTAGGGAAACTAAATATA+AGG 0.364114 8_1:-88997409 Msa1224200:CDS
TATGTTACCTTAGATTAGAT+TGG 0.375634 8_1:+88997366 None:intergenic
CAAGCATGGAAGCACTTTCA+TGG 0.384069 8_1:-88998175 Msa1224200:CDS
AATAAAACCACAACCACAAC+TGG 0.394819 8_1:+88998421 None:intergenic
TCTTGAATATCATGCTTAGT+TGG 0.411358 8_1:+88998479 None:intergenic
TTGGAATTAGTTTGACTTGA+GGG 0.414606 8_1:+88998096 None:intergenic
AGAGAAACAGTAGTTGTAGA+AGG 0.431084 8_1:+88998131 None:intergenic
AAAGCGTAACATACAGGTTA+AGG 0.436501 8_1:-88997483 Msa1224200:CDS
GAAATCTCCAGTTGTGGTTG+TGG 0.445667 8_1:-88998428 Msa1224200:CDS
GGTAGAAATGATGCTTATTG+AGG 0.452714 8_1:-88997462 Msa1224200:CDS
GAAGCCATTGTGGAACTTGT+AGG 0.460657 8_1:-88997425 Msa1224200:CDS
GTTTGAGATAAATTTGAAGA+AGG 0.464932 8_1:-88998330 Msa1224200:CDS
AATATAAGGAAATTGGTGAT+TGG 0.465583 8_1:-88997395 Msa1224200:CDS
ATGTTACCTTAGATTAGATT+GGG 0.474526 8_1:+88997367 None:intergenic
AGGTGTCACGGCATGTTTCA+AGG 0.477649 8_1:+88998151 None:intergenic
GTAGAAATGATGCTTATTGA+GGG 0.483302 8_1:-88997461 Msa1224200:CDS
TATAGATGATGTTGTTTATG+TGG 0.505857 8_1:-88998222 Msa1224200:CDS
TAATGAAAAGCGTAACATAC+AGG 0.506176 8_1:-88997489 Msa1224200:CDS
AATTTCGGAGACGACGATGA+TGG 0.516786 8_1:-88998368 Msa1224200:CDS
AAGGAGTAGTTACATGGAGT+TGG 0.519333 8_1:+88998077 None:intergenic
CGAAATTGTAATCAAACACT+TGG 0.523720 8_1:+88998384 None:intergenic
ATTGGATTCAATAAGAGAAG+AGG 0.530259 8_1:-88998300 Msa1224200:CDS
AAGCCATTGTGGAACTTGTA+GGG 0.539241 8_1:-88997424 Msa1224200:CDS
TATGTGGCAGTGGGAACAAA+TGG 0.556548 8_1:-88998206 Msa1224200:CDS
TGATGTTGTTTATGTGGCAG+TGG 0.557388 8_1:-88998216 Msa1224200:CDS
TAAGACAAGGAGTAGTTACA+TGG 0.583206 8_1:+88998071 None:intergenic
CAACGTTGCTGAAGCCATTG+TGG 0.587024 8_1:-88997435 Msa1224200:CDS
GTTGGAATTAGTTTGACTTG+AGG 0.589120 8_1:+88998095 None:intergenic
TGACTTGAGGGAAAACGTGT+AGG 0.604542 8_1:+88998108 None:intergenic
AAATGGTGGAAGTTCAAGCA+TGG 0.604604 8_1:-88998189 Msa1224200:CDS
TTTCCCTACAAGTTCCACAA+TGG 0.638145 8_1:+88997421 None:intergenic
GATGTTGTTTATGTGGCAGT+GGG 0.642705 8_1:-88998215 Msa1224200:CDS
AGTAGTTGTAGAAGGTGTCA+CGG 0.650946 8_1:+88998139 None:intergenic
ATTCAATAAGAGAAGAGGAG+TGG 0.652515 8_1:-88998295 Msa1224200:CDS
GTGGCAGTGGGAACAAATGG+TGG 0.662195 8_1:-88998203 Msa1224200:CDS
GATGATGAAATCTCCAGTTG+TGG 0.697511 8_1:-88998434 Msa1224200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTAGATAAAGTTAAATT+TGG + chr8_1:88997868-88997887 None:intergenic 15.0%
!!! TGACAATAATATGTTTTTTT+TGG - chr8_1:88998049-88998068 Msa1224200:intron 15.0%
!!! AAAACTTATTTTGAATGTTA+TGG + chr8_1:88998122-88998141 None:intergenic 15.0%
!! CAATTAGATAAAGTTAAATT+TGG + chr8_1:88997868-88997887 None:intergenic 15.0%
!!! TGACAATAATATGTTTTTTT+TGG - chr8_1:88998049-88998068 Msa1224200:intron 15.0%
!!! AAAACTTATTTTGAATGTTA+TGG + chr8_1:88998122-88998141 None:intergenic 15.0%
!!! TTTTGTTTGAAAATAAGACA+AGG + chr8_1:88997806-88997825 None:intergenic 20.0%
!!! AATGTTATGGTTATGTTTTT+GGG + chr8_1:88998109-88998128 None:intergenic 20.0%
!! TTTCATAATCTTAAACAACT+TGG - chr8_1:88998151-88998170 Msa1224200:CDS 20.0%
!! AAACCTTATATATGTATTGA+TGG - chr8_1:88998324-88998343 Msa1224200:CDS 20.0%
!! GAAACTAAATATAAGGAAAT+TGG - chr8_1:88998459-88998478 Msa1224200:CDS 20.0%
!!! TTTTGTTTGAAAATAAGACA+AGG + chr8_1:88997806-88997825 None:intergenic 20.0%
!!! AATGTTATGGTTATGTTTTT+GGG + chr8_1:88998109-88998128 None:intergenic 20.0%
!! TTTCATAATCTTAAACAACT+TGG - chr8_1:88998151-88998170 Msa1224200:CDS 20.0%
!! AAACCTTATATATGTATTGA+TGG - chr8_1:88998324-88998343 Msa1224200:CDS 20.0%
!! GAAACTAAATATAAGGAAAT+TGG - chr8_1:88998459-88998478 Msa1224200:CDS 20.0%
! CAAGTGTTTGATTACAATTT+CGG - chr8_1:88997478-88997497 Msa1224200:CDS 25.0%
!! GTTTGAGATAAATTTGAAGA+AGG - chr8_1:88997531-88997550 Msa1224200:intron 25.0%
!! TATAGATGATGTTGTTTATG+TGG - chr8_1:88997639-88997658 Msa1224200:intron 25.0%
! TATGATGTGCAATTAATAAG+AGG + chr8_1:88997908-88997927 None:intergenic 25.0%
!! AAAGATTTGATCACATCTTT+TGG + chr8_1:88997997-88998016 None:intergenic 25.0%
!! GAATGTTATGGTTATGTTTT+TGG + chr8_1:88998110-88998129 None:intergenic 25.0%
! GAAGCTATATAGTATGATTT+AGG + chr8_1:88998195-88998214 None:intergenic 25.0%
! ACTCCATCAATACATATATA+AGG + chr8_1:88998330-88998349 None:intergenic 25.0%
! TTGTAGGGAAACTAAATATA+AGG - chr8_1:88998452-88998471 Msa1224200:CDS 25.0%
!! AATATAAGGAAATTGGTGAT+TGG - chr8_1:88998466-88998485 Msa1224200:CDS 25.0%
! CAAGTGTTTGATTACAATTT+CGG - chr8_1:88997478-88997497 Msa1224200:CDS 25.0%
!! GTTTGAGATAAATTTGAAGA+AGG - chr8_1:88997531-88997550 Msa1224200:intron 25.0%
!! TATAGATGATGTTGTTTATG+TGG - chr8_1:88997639-88997658 Msa1224200:intron 25.0%
! TATGATGTGCAATTAATAAG+AGG + chr8_1:88997908-88997927 None:intergenic 25.0%
!! AAAGATTTGATCACATCTTT+TGG + chr8_1:88997997-88998016 None:intergenic 25.0%
!! GAATGTTATGGTTATGTTTT+TGG + chr8_1:88998110-88998129 None:intergenic 25.0%
! GAAGCTATATAGTATGATTT+AGG + chr8_1:88998195-88998214 None:intergenic 25.0%
! ACTCCATCAATACATATATA+AGG + chr8_1:88998330-88998349 None:intergenic 25.0%
! TTGTAGGGAAACTAAATATA+AGG - chr8_1:88998452-88998471 Msa1224200:CDS 25.0%
!! AATATAAGGAAATTGGTGAT+TGG - chr8_1:88998466-88998485 Msa1224200:CDS 25.0%
TCTTGAATATCATGCTTAGT+TGG + chr8_1:88997385-88997404 None:intergenic 30.0%
CGAAATTGTAATCAAACACT+TGG + chr8_1:88997480-88997499 None:intergenic 30.0%
TTTGAAGAAGGAAAGTAGAT+TGG - chr8_1:88997543-88997562 Msa1224200:intron 30.0%
ATTGGATTCAATAAGAGAAG+AGG - chr8_1:88997561-88997580 Msa1224200:intron 30.0%
!! TTGGAATTAGTTTGACTTGA+GGG + chr8_1:88997768-88997787 None:intergenic 30.0%
! TTGGTGATTTATGCTTATGT+TGG + chr8_1:88997849-88997868 None:intergenic 30.0%
CATGAATTATTACTACGAGA+AGG - chr8_1:88998214-88998233 Msa1224200:CDS 30.0%
!! GCTTCATGTATTATTTCTGA+AGG + chr8_1:88998265-88998284 None:intergenic 30.0%
! TAATGAAAAGCGTAACATAC+AGG - chr8_1:88998372-88998391 Msa1224200:CDS 30.0%
GTAGAAATGATGCTTATTGA+GGG - chr8_1:88998400-88998419 Msa1224200:CDS 30.0%
TCTTGAATATCATGCTTAGT+TGG + chr8_1:88997385-88997404 None:intergenic 30.0%
CGAAATTGTAATCAAACACT+TGG + chr8_1:88997480-88997499 None:intergenic 30.0%
TTTGAAGAAGGAAAGTAGAT+TGG - chr8_1:88997543-88997562 Msa1224200:intron 30.0%
ATTGGATTCAATAAGAGAAG+AGG - chr8_1:88997561-88997580 Msa1224200:intron 30.0%
!! TTGGAATTAGTTTGACTTGA+GGG + chr8_1:88997768-88997787 None:intergenic 30.0%
! TTGGTGATTTATGCTTATGT+TGG + chr8_1:88997849-88997868 None:intergenic 30.0%
CATGAATTATTACTACGAGA+AGG - chr8_1:88998214-88998233 Msa1224200:CDS 30.0%
!! GCTTCATGTATTATTTCTGA+AGG + chr8_1:88998265-88998284 None:intergenic 30.0%
! TAATGAAAAGCGTAACATAC+AGG - chr8_1:88998372-88998391 Msa1224200:CDS 30.0%
GTAGAAATGATGCTTATTGA+GGG - chr8_1:88998400-88998419 Msa1224200:CDS 30.0%
AATAAAACCACAACCACAAC+TGG + chr8_1:88997443-88997462 None:intergenic 35.0%
!!! AGTTGTGGTTGTGGTTTTAT+TGG - chr8_1:88997442-88997461 Msa1224200:CDS 35.0%
!!! GTTGTGGTTGTGGTTTTATT+GGG - chr8_1:88997443-88997462 Msa1224200:CDS 35.0%
ATTCAATAAGAGAAGAGGAG+TGG - chr8_1:88997566-88997585 Msa1224200:intron 35.0%
AGAGAAACAGTAGTTGTAGA+AGG + chr8_1:88997733-88997752 None:intergenic 35.0%
!! GTTGGAATTAGTTTGACTTG+AGG + chr8_1:88997769-88997788 None:intergenic 35.0%
TAAGACAAGGAGTAGTTACA+TGG + chr8_1:88997793-88997812 None:intergenic 35.0%
GTGATTTATGCTTATGTTGG+AGG + chr8_1:88997846-88997865 None:intergenic 35.0%
AAAGCGTAACATACAGGTTA+AGG - chr8_1:88998378-88998397 Msa1224200:CDS 35.0%
GGTAGAAATGATGCTTATTG+AGG - chr8_1:88998399-88998418 Msa1224200:CDS 35.0%
AATAAAACCACAACCACAAC+TGG + chr8_1:88997443-88997462 None:intergenic 35.0%
!!! AGTTGTGGTTGTGGTTTTAT+TGG - chr8_1:88997442-88997461 Msa1224200:CDS 35.0%
!!! GTTGTGGTTGTGGTTTTATT+GGG - chr8_1:88997443-88997462 Msa1224200:CDS 35.0%
ATTCAATAAGAGAAGAGGAG+TGG - chr8_1:88997566-88997585 Msa1224200:intron 35.0%
AGAGAAACAGTAGTTGTAGA+AGG + chr8_1:88997733-88997752 None:intergenic 35.0%
!! GTTGGAATTAGTTTGACTTG+AGG + chr8_1:88997769-88997788 None:intergenic 35.0%
TAAGACAAGGAGTAGTTACA+TGG + chr8_1:88997793-88997812 None:intergenic 35.0%
GTGATTTATGCTTATGTTGG+AGG + chr8_1:88997846-88997865 None:intergenic 35.0%
AAAGCGTAACATACAGGTTA+AGG - chr8_1:88998378-88998397 Msa1224200:CDS 35.0%
GGTAGAAATGATGCTTATTG+AGG - chr8_1:88998399-88998418 Msa1224200:CDS 35.0%
GATGATGAAATCTCCAGTTG+TGG - chr8_1:88997427-88997446 Msa1224200:CDS 40.0%
! TGATGTTGTTTATGTGGCAG+TGG - chr8_1:88997645-88997664 Msa1224200:intron 40.0%
! GATGTTGTTTATGTGGCAGT+GGG - chr8_1:88997646-88997665 Msa1224200:intron 40.0%
! AAATGGTGGAAGTTCAAGCA+TGG - chr8_1:88997672-88997691 Msa1224200:intron 40.0%
!! AGTAGTTGTAGAAGGTGTCA+CGG + chr8_1:88997725-88997744 None:intergenic 40.0%
AAGGAGTAGTTACATGGAGT+TGG + chr8_1:88997787-88997806 None:intergenic 40.0%
AGAAGGTTTCATGAAGCAAG+AGG - chr8_1:88998231-88998250 Msa1224200:CDS 40.0%
AAGCCATTGTGGAACTTGTA+GGG - chr8_1:88998437-88998456 Msa1224200:CDS 40.0%
TTTCCCTACAAGTTCCACAA+TGG + chr8_1:88998443-88998462 None:intergenic 40.0%
GATGATGAAATCTCCAGTTG+TGG - chr8_1:88997427-88997446 Msa1224200:CDS 40.0%
! TGATGTTGTTTATGTGGCAG+TGG - chr8_1:88997645-88997664 Msa1224200:intron 40.0%
! GATGTTGTTTATGTGGCAGT+GGG - chr8_1:88997646-88997665 Msa1224200:intron 40.0%
! AAATGGTGGAAGTTCAAGCA+TGG - chr8_1:88997672-88997691 Msa1224200:intron 40.0%
!! AGTAGTTGTAGAAGGTGTCA+CGG + chr8_1:88997725-88997744 None:intergenic 40.0%
AAGGAGTAGTTACATGGAGT+TGG + chr8_1:88997787-88997806 None:intergenic 40.0%
AGAAGGTTTCATGAAGCAAG+AGG - chr8_1:88998231-88998250 Msa1224200:CDS 40.0%
AAGCCATTGTGGAACTTGTA+GGG - chr8_1:88998437-88998456 Msa1224200:CDS 40.0%
TTTCCCTACAAGTTCCACAA+TGG + chr8_1:88998443-88998462 None:intergenic 40.0%
GAAATCTCCAGTTGTGGTTG+TGG - chr8_1:88997433-88997452 Msa1224200:CDS 45.0%
AATTTCGGAGACGACGATGA+TGG - chr8_1:88997493-88997512 Msa1224200:CDS 45.0%
TATGTGGCAGTGGGAACAAA+TGG - chr8_1:88997655-88997674 Msa1224200:intron 45.0%
!! CAAGCATGGAAGCACTTTCA+TGG - chr8_1:88997686-88997705 Msa1224200:intron 45.0%
TGACTTGAGGGAAAACGTGT+AGG + chr8_1:88997756-88997775 None:intergenic 45.0%
GAAGCCATTGTGGAACTTGT+AGG - chr8_1:88998436-88998455 Msa1224200:CDS 45.0%
GAAATCTCCAGTTGTGGTTG+TGG - chr8_1:88997433-88997452 Msa1224200:CDS 45.0%
AATTTCGGAGACGACGATGA+TGG - chr8_1:88997493-88997512 Msa1224200:CDS 45.0%
TATGTGGCAGTGGGAACAAA+TGG - chr8_1:88997655-88997674 Msa1224200:intron 45.0%
!! CAAGCATGGAAGCACTTTCA+TGG - chr8_1:88997686-88997705 Msa1224200:intron 45.0%
TGACTTGAGGGAAAACGTGT+AGG + chr8_1:88997756-88997775 None:intergenic 45.0%
GAAGCCATTGTGGAACTTGT+AGG - chr8_1:88998436-88998455 Msa1224200:CDS 45.0%
! AGGTGTCACGGCATGTTTCA+AGG + chr8_1:88997713-88997732 None:intergenic 50.0%
CAACGTTGCTGAAGCCATTG+TGG - chr8_1:88998426-88998445 Msa1224200:CDS 50.0%
! AGGTGTCACGGCATGTTTCA+AGG + chr8_1:88997713-88997732 None:intergenic 50.0%
CAACGTTGCTGAAGCCATTG+TGG - chr8_1:88998426-88998445 Msa1224200:CDS 50.0%
GTGGCAGTGGGAACAAATGG+TGG - chr8_1:88997658-88997677 Msa1224200:intron 55.0%
GTGGCAGTGGGAACAAATGG+TGG - chr8_1:88997658-88997677 Msa1224200:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 88997376 88998507 88997376 ID=Msa1224200;Name=Msa1224200
chr8_1 mRNA 88997376 88998507 88997376 ID=Msa1224200-mRNA-1;Parent=Msa1224200;Name=Msa1224200-mRNA-1;_AED=0.04;_eAED=0.06;_QI=0|0|0|1|0|0|2|0|193
chr8_1 exon 88998077 88998507 88998077 ID=Msa1224200-mRNA-1:exon:32369;Parent=Msa1224200-mRNA-1
chr8_1 exon 88997376 88997523 88997376 ID=Msa1224200-mRNA-1:exon:32368;Parent=Msa1224200-mRNA-1
chr8_1 CDS 88998077 88998507 88998077 ID=Msa1224200-mRNA-1:cds;Parent=Msa1224200-mRNA-1
chr8_1 CDS 88997376 88997523 88997376 ID=Msa1224200-mRNA-1:cds;Parent=Msa1224200-mRNA-1
Gene Sequence

>Msa1224200

ATGATGCCAACTAAGCATGATATTCAAGATGTTCAGTGCTTGCACTACTACGATGATGAAATCTCCAGTTGTGGTTGTGGTTTTATTGGGTTCATGAAAAGCCAAGTGTTTGATTACAATTTCGGAGACGACGATGATGGTGATTCAGAAGAGCTGTTTGAGATAAATTTGAAGAAGGAAAGTAGATTGGATTCAATAAGAGAAGAGGAGTGGTGTGAGAGCAGTACTGTGTTCTCTCTTGATATACACAACAATGATAATAATATAGATGATGTTGTTTATGTGGCAGTGGGAACAAATGGTGGAAGTTCAAGCATGGAAGCACTTTCATGGACCTTGAAACATGCCGTGACACCTTCTACAACTACTGTTTCTCTCCTACACGTTTTCCCTCAAGTCAAACTAATTCCAACTCCATGTAACTACTCCTTTACTAGTAGTAATAATGAAAAGCGTAACATACAGGTTAAGGTAGAAATGATGCTTATTGAGGGTGACAACGTTGCTGAAGCCATTGTGGAACTTGTAGGGAAACTAAATATAAGGAAATTGGTGATTGGAACTACCCAATCTAATCTA

Protein sequence

>Msa1224200

MMPTKHDIQDVQCLHYYDDEISSCGCGFIGFMKSQVFDYNFGDDDDGDSEELFEINLKKESRLDSIREEEWCESSTVFSLDIHNNDNNIDDVVYVAVGTNGGSSSMEALSWTLKHAVTPSTTTVSLLHVFPQVKLIPTPCNYSFTSSNNEKRNIQVKVEMMLIEGDNVAEAIVELVGKLNIRKLVIGTTQSNL