Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0219310 | XP_003596854.1 | 90.909 | 110 | 10 | 0 | 1 | 110 | 1 | 110 | 6.07e-49 | 161 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0219310 | sp|Q9SRN4|GIR2_ARATH | 41.593 | 113 | 43 | 4 | 1 | 108 | 1 | 95 | 7.78e-16 | 70.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0219310 | G7IHG7 | 90.909 | 110 | 10 | 0 | 1 | 110 | 1 | 110 | 2.90e-49 | 161 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005050 | Msa0219310 | 0.816616 | 5.045405e-52 | -8.615850e-47 |
| Msa0012440 | Msa0219310 | 0.804496 | 2.100676e-49 | -8.615850e-47 |
| Msa0022640 | Msa0219310 | 0.801455 | 8.932075e-49 | -8.615850e-47 |
| Msa0036430 | Msa0219310 | 0.816738 | 4.737438e-52 | -8.615850e-47 |
| Msa0053110 | Msa0219310 | 0.831340 | 1.764944e-55 | -8.615850e-47 |
| Msa0084500 | Msa0219310 | 0.812733 | 3.653918e-51 | -8.615850e-47 |
| Msa0098440 | Msa0219310 | 0.826911 | 2.092330e-54 | -8.615850e-47 |
| Msa0106790 | Msa0219310 | 0.801650 | 8.147052e-49 | -8.615850e-47 |
| Msa0118400 | Msa0219310 | 0.801986 | 6.948800e-49 | -8.615850e-47 |
| Msa0130570 | Msa0219310 | 0.811120 | 8.208191e-51 | -8.615850e-47 |
| Msa0141600 | Msa0219310 | 0.804911 | 1.720926e-49 | -8.615850e-47 |
| Msa0161680 | Msa0219310 | 0.805912 | 1.061538e-49 | -8.615850e-47 |
| Msa0176470 | Msa0219310 | 0.816575 | 5.153806e-52 | -8.615850e-47 |
| Msa0181090 | Msa0219310 | 0.801215 | 1.000358e-48 | -8.615850e-47 |
| Msa0203030 | Msa0219310 | 0.831360 | 1.744220e-55 | -8.615850e-47 |
| Msa0219190 | Msa0219310 | 0.820055 | 8.396993e-53 | -8.615850e-47 |
| Msa0219310 | Msa0258940 | 0.955209 | 5.129466e-113 | -8.615850e-47 |
| Msa0219310 | Msa0298240 | 0.804444 | 2.153684e-49 | -8.615850e-47 |
| Msa0219310 | Msa0300620 | 0.812054 | 5.141599e-51 | -8.615850e-47 |
| Msa0219310 | Msa0314630 | 0.802019 | 6.842203e-49 | -8.615850e-47 |
| Msa0219310 | Msa0322830 | 0.810620 | 1.052878e-50 | -8.615850e-47 |
| Msa0219310 | Msa0322840 | 0.803170 | 3.961857e-49 | -8.615850e-47 |
| Msa0219310 | Msa0337850 | 0.807004 | 6.247304e-50 | -8.615850e-47 |
| Msa0219310 | Msa0413710 | 0.808777 | 2.622068e-50 | -8.615850e-47 |
| Msa0219310 | Msa0413930 | 0.839603 | 1.435693e-57 | -8.615850e-47 |
| Msa0219310 | Msa0436460 | 0.800447 | 1.435054e-48 | -8.615850e-47 |
| Msa0219310 | Msa0436500 | 0.810547 | 1.092057e-50 | -8.615850e-47 |
| Msa0219310 | Msa0575250 | 0.803606 | 3.217397e-49 | -8.615850e-47 |
| Msa0219310 | Msa0682530 | 0.800825 | 1.201650e-48 | -8.615850e-47 |
| Msa0219310 | Msa0691160 | 0.805618 | 1.223554e-49 | -8.615850e-47 |
| Msa0219310 | Msa0707220 | 0.804547 | 2.050305e-49 | -8.615850e-47 |
| Msa0219310 | Msa0747780 | 0.830746 | 2.469286e-55 | -8.615850e-47 |
| Msa0219310 | Msa0872310 | 0.801653 | 8.134344e-49 | -8.615850e-47 |
| Msa0219310 | Msa0873190 | 0.806813 | 6.853915e-50 | -8.615850e-47 |
| Msa0219310 | Msa0885060 | 0.816039 | 6.790335e-52 | -8.615850e-47 |
| Msa0219310 | Msa0905550 | 0.819540 | 1.100579e-52 | -8.615850e-47 |
| Msa0219310 | Msa0937550 | 0.801119 | 1.046628e-48 | -8.615850e-47 |
| Msa0219310 | Msa0938430 | 0.801674 | 8.054363e-49 | -8.615850e-47 |
| Msa0219310 | Msa1033560 | 0.835144 | 1.991221e-56 | -8.615850e-47 |
| Msa0219310 | Msa1055260 | 0.809854 | 1.539847e-50 | -8.615850e-47 |
| Msa0219310 | Msa1055280 | 0.811938 | 5.451095e-51 | -8.615850e-47 |
| Msa0219310 | Msa1055500 | 0.826356 | 2.838083e-54 | -8.615850e-47 |
| Msa0219310 | Msa1073340 | 0.832030 | 1.192881e-55 | -8.615850e-47 |
| Msa0219310 | Msa1113850 | 0.816236 | 6.136179e-52 | -8.615850e-47 |
| Msa0219310 | Msa1145020 | 0.815456 | 9.158075e-52 | -8.615850e-47 |
| Msa0219310 | Msa1321000 | 0.825180 | 5.394620e-54 | -8.615850e-47 |
| Msa0219310 | Msa1375940 | 0.810195 | 1.300260e-50 | -8.615850e-47 |
| Msa0219310 | Msa1379990 | 0.818695 | 1.714388e-52 | -8.615850e-47 |
| Msa0219310 | Msa1420060 | 0.835346 | 1.770530e-56 | -8.615850e-47 |
| Msa0219310 | Msa1424690 | 0.801152 | 1.030428e-48 | -8.615850e-47 |
| Msa0219310 | Msa1429420 | 0.804899 | 1.730491e-49 | -8.615850e-47 |
| Msa0219310 | Msa1435670 | 0.806546 | 7.805399e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0219310 | MtrunA17_Chr2g0321781 | 90.909 | 110 | 10 | 0 | 1 | 110 | 1 | 110 | 5.58e-53 | 161 |
| Msa0219310 | MtrunA17_Chr2g0321771 | 55.140 | 107 | 32 | 4 | 1 | 107 | 1 | 91 | 1.58e-27 | 96.7 |
| Msa0219310 | MtrunA17_Chr1g0177991 | 55.769 | 52 | 22 | 1 | 60 | 110 | 57 | 108 | 1.22e-15 | 67.0 |
| Msa0219310 | MtrunA17_Chr7g0267071 | 63.158 | 38 | 14 | 0 | 73 | 110 | 57 | 94 | 2.82e-14 | 63.2 |
| Msa0219310 | MtrunA17_Chr4g0025941 | 58.537 | 41 | 17 | 0 | 70 | 110 | 54 | 94 | 3.22e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0219310 | AT3G11600.1 | 41.593 | 113 | 43 | 4 | 1 | 108 | 1 | 95 | 7.91e-17 | 70.5 |
| Msa0219310 | AT5G06270.1 | 58.000 | 50 | 21 | 0 | 59 | 108 | 52 | 101 | 4.66e-15 | 66.2 |
| Msa0219310 | AT5G06270.2 | 58.000 | 50 | 21 | 0 | 59 | 108 | 52 | 101 | 4.66e-15 | 66.2 |
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAACATACATGTAACATTT+AGG | 0.333558 | 2_1:+66833053 | None:intergenic |
| TCATCAGTTGATCGCTTGCC+GGG | 0.363679 | 2_1:-66833224 | Msa0219310:CDS |
| ATCATCAGTTGATCGCTTGC+CGG | 0.374132 | 2_1:-66833225 | Msa0219310:CDS |
| AGTTGATCGCTTGCCGGGGT+TGG | 0.458397 | 2_1:-66833219 | Msa0219310:CDS |
| GATGATGATTTCGATTCCTC+TGG | 0.472844 | 2_1:+66833242 | None:intergenic |
| ACAAAGGAGATGGTTCTTGT+TGG | 0.493804 | 2_1:-66833080 | Msa0219310:CDS |
| GTATTACACTTGGGACACTT+TGG | 0.514234 | 2_1:+66833014 | None:intergenic |
| AAGATGATTGCTCATGATCA+AGG | 0.518003 | 2_1:-66833155 | Msa0219310:CDS |
| GATTTCGATTCCTCTGGTGA+TGG | 0.559583 | 2_1:+66833248 | None:intergenic |
| CAAAAGAAAGAGTCGAGGGT+TGG | 0.561959 | 2_1:-66833282 | Msa0219310:CDS |
| AGAGTCGAGGGTTGGAATTG+AGG | 0.565433 | 2_1:-66833274 | Msa0219310:CDS |
| GAACATGGTGACACCAACCC+CGG | 0.606282 | 2_1:+66833206 | None:intergenic |
| TCATGATCAAGGAGCCTCGA+CGG | 0.609175 | 2_1:-66833144 | Msa0219310:CDS |
| CATCAGTTGATCGCTTGCCG+GGG | 0.609861 | 2_1:-66833223 | Msa0219310:CDS |
| AAACGCAGTTACAAAGGAGA+TGG | 0.616098 | 2_1:-66833090 | Msa0219310:CDS |
| AATAACAGTAGTATTACACT+TGG | 0.622685 | 2_1:+66833004 | None:intergenic |
| GTATGCAAACGCAGTTACAA+AGG | 0.622761 | 2_1:-66833096 | Msa0219310:CDS |
| TGAGCAAAAGAAAGAGTCGA+GGG | 0.626972 | 2_1:-66833286 | Msa0219310:CDS |
| ATGAGCAAAAGAAAGAGTCG+AGG | 0.641115 | 2_1:-66833287 | Msa0219310:CDS |
| TGAATATGATACTGAAGATG+AGG | 0.660765 | 2_1:+66833181 | None:intergenic |
| ATTGAGGCATCCATCACCAG+AGG | 0.697008 | 2_1:-66833258 | Msa0219310:CDS |
| ATAACAGTAGTATTACACTT+GGG | 0.700958 | 2_1:+66833005 | None:intergenic |
| ACTGAAGATGAGGATGAACA+TGG | 0.728056 | 2_1:+66833191 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACATACATGTAACATTT+AGG | + | chr2_1:66833214-66833233 | None:intergenic | 20.0% |
| ! | ATAACAGTAGTATTACACTT+GGG | + | chr2_1:66833262-66833281 | None:intergenic | 25.0% |
| ! | AATAACAGTAGTATTACACT+TGG | + | chr2_1:66833263-66833282 | None:intergenic | 25.0% |
| TGAATATGATACTGAAGATG+AGG | + | chr2_1:66833086-66833105 | None:intergenic | 30.0% | |
| ! | TATTCTTGATCTTTTCAGAG+AGG | - | chr2_1:66833279-66833298 | Msa0219310:CDS | 30.0% |
| AAGATGATTGCTCATGATCA+AGG | - | chr2_1:66833109-66833128 | Msa0219310:CDS | 35.0% | |
| ATGAGCAAAAGAAAGAGTCG+AGG | - | chr2_1:66832977-66832996 | Msa0219310:CDS | 40.0% | |
| TGAGCAAAAGAAAGAGTCGA+GGG | - | chr2_1:66832978-66832997 | Msa0219310:CDS | 40.0% | |
| GATGATGATTTCGATTCCTC+TGG | + | chr2_1:66833025-66833044 | None:intergenic | 40.0% | |
| ACTGAAGATGAGGATGAACA+TGG | + | chr2_1:66833076-66833095 | None:intergenic | 40.0% | |
| GTATGCAAACGCAGTTACAA+AGG | - | chr2_1:66833168-66833187 | Msa0219310:CDS | 40.0% | |
| AAACGCAGTTACAAAGGAGA+TGG | - | chr2_1:66833174-66833193 | Msa0219310:CDS | 40.0% | |
| ACAAAGGAGATGGTTCTTGT+TGG | - | chr2_1:66833184-66833203 | Msa0219310:CDS | 40.0% | |
| GTATTACACTTGGGACACTT+TGG | + | chr2_1:66833253-66833272 | None:intergenic | 40.0% | |
| CAAAAGAAAGAGTCGAGGGT+TGG | - | chr2_1:66832982-66833001 | Msa0219310:CDS | 45.0% | |
| GATTTCGATTCCTCTGGTGA+TGG | + | chr2_1:66833019-66833038 | None:intergenic | 45.0% | |
| ATCATCAGTTGATCGCTTGC+CGG | - | chr2_1:66833039-66833058 | Msa0219310:CDS | 45.0% | |
| ! | ATCTTCTTTTGTCTCCGTCG+AGG | + | chr2_1:66833137-66833156 | None:intergenic | 45.0% |
| AGAGTCGAGGGTTGGAATTG+AGG | - | chr2_1:66832990-66833009 | Msa0219310:CDS | 50.0% | |
| ! | ATTGAGGCATCCATCACCAG+AGG | - | chr2_1:66833006-66833025 | Msa0219310:CDS | 50.0% |
| TCATCAGTTGATCGCTTGCC+GGG | - | chr2_1:66833040-66833059 | Msa0219310:CDS | 50.0% | |
| TCATGATCAAGGAGCCTCGA+CGG | - | chr2_1:66833120-66833139 | Msa0219310:CDS | 50.0% | |
| CATCAGTTGATCGCTTGCCG+GGG | - | chr2_1:66833041-66833060 | Msa0219310:CDS | 55.0% | |
| GAACATGGTGACACCAACCC+CGG | + | chr2_1:66833061-66833080 | None:intergenic | 55.0% | |
| AGTTGATCGCTTGCCGGGGT+TGG | - | chr2_1:66833045-66833064 | Msa0219310:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 66832977 | 66833309 | 66832977 | ID=Msa0219310;Name=Msa0219310 |
| chr2_1 | mRNA | 66832977 | 66833309 | 66832977 | ID=Msa0219310-mRNA-1;Parent=Msa0219310;Name=Msa0219310-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|110 |
| chr2_1 | exon | 66832977 | 66833309 | 66832977 | ID=Msa0219310-mRNA-1:exon:18874;Parent=Msa0219310-mRNA-1 |
| chr2_1 | CDS | 66832977 | 66833309 | 66832977 | ID=Msa0219310-mRNA-1:cds;Parent=Msa0219310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
