AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1055500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1055500 MtrunA17_Chr7g0227021 68.235 85 19 2 1 85 1 77 5.02e-31 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGGAAGGCACAAATGTTT+TGG 0.358210 7_2:+29989921 None:intergenic
TTATGGGAGAAGATTCCTTC+AGG 0.401427 7_2:+29989534 None:intergenic
ATTTCCCCTCCTTTAACAAA+AGG 0.422854 7_2:-29989799 Msa1055500:CDS
TATAGGCAAATTAGAGCTAA+AGG 0.453977 7_2:+29989883 None:intergenic
TGAGTGATGGGAGTGTAAAA+TGG 0.454963 7_2:+29989838 None:intergenic
GGGAAATAGAAAGTGAAGAT+TGG 0.478384 7_2:+29989815 None:intergenic
AAGTGAAGATTGGTGAGTGA+TGG 0.499564 7_2:+29989825 None:intergenic
ATGAGGAGAAGTAAAAGTAT+AGG 0.508071 7_2:+29989866 None:intergenic
AGTGAAGATTGGTGAGTGAT+GGG 0.518584 7_2:+29989826 None:intergenic
TTTAATAATTAAAATGCTTG+TGG 0.520572 7_2:+29989467 None:intergenic
AGTGTAAAATGGAGTAAATG+AGG 0.541294 7_2:+29989849 None:intergenic
AGGTAGCAGAGAATAGAAAG+TGG 0.576522 7_2:+29989903 None:intergenic
AGCAGAGAATAGAAAGTGGA+AGG 0.585262 7_2:+29989907 None:intergenic
AGACAATAGTTGAGATTACA+CGG 0.591351 7_2:+29989501 None:intergenic
TTAAGAGTGTCACAACCTGA+AGG 0.594460 7_2:-29989549 Msa1055500:intron
GACAATAGTTGAGATTACAC+GGG 0.693195 7_2:+29989502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAATTTACCTTTTGTTAA+AGG + chr7_2:29989646-29989665 None:intergenic 20.0%
!! TATGATATAAACATAGAAGA+TGG + chr7_2:29989702-29989721 None:intergenic 20.0%
!! ATCTTCTATGTTTATATCAT+AGG - chr7_2:29989701-29989720 Msa1055500:intron 20.0%
!!! AATTTGATCGATATTTTTGA+TGG - chr7_2:29989789-29989808 Msa1055500:CDS 20.0%
!!! ATTTGATCGATATTTTTGAT+GGG - chr7_2:29989790-29989809 Msa1055500:CDS 20.0%
!! ATTTACCTTTTGTTAAAGGA+GGG + chr7_2:29989642-29989661 None:intergenic 25.0%
!! AATTTACCTTTTGTTAAAGG+AGG + chr7_2:29989643-29989662 None:intergenic 25.0%
!!! TTTGATCGATATTTTTGATG+GGG - chr7_2:29989791-29989810 Msa1055500:CDS 25.0%
TATAGGCAAATTAGAGCTAA+AGG + chr7_2:29989553-29989572 None:intergenic 30.0%
! ATGAGGAGAAGTAAAAGTAT+AGG + chr7_2:29989570-29989589 None:intergenic 30.0%
AGTGTAAAATGGAGTAAATG+AGG + chr7_2:29989587-29989606 None:intergenic 30.0%
! TTTACCTTTTGTTAAAGGAG+GGG + chr7_2:29989641-29989660 None:intergenic 30.0%
!! TTGATCGATATTTTTGATGG+GGG - chr7_2:29989792-29989811 Msa1055500:CDS 30.0%
AGACAATAGTTGAGATTACA+CGG + chr7_2:29989935-29989954 None:intergenic 30.0%
GGGAAATAGAAAGTGAAGAT+TGG + chr7_2:29989621-29989640 None:intergenic 35.0%
ATTTCCCCTCCTTTAACAAA+AGG - chr7_2:29989634-29989653 Msa1055500:CDS 35.0%
! ACACGGGAGAAAACTTTTAT+GGG + chr7_2:29989918-29989937 None:intergenic 35.0%
! TACACGGGAGAAAACTTTTA+TGG + chr7_2:29989919-29989938 None:intergenic 35.0%
GACAATAGTTGAGATTACAC+GGG + chr7_2:29989934-29989953 None:intergenic 35.0%
!! AGTGGAAGGCACAAATGTTT+TGG + chr7_2:29989515-29989534 None:intergenic 40.0%
AGCAGAGAATAGAAAGTGGA+AGG + chr7_2:29989529-29989548 None:intergenic 40.0%
AGGTAGCAGAGAATAGAAAG+TGG + chr7_2:29989533-29989552 None:intergenic 40.0%
! TGAGTGATGGGAGTGTAAAA+TGG + chr7_2:29989598-29989617 None:intergenic 40.0%
AGTGAAGATTGGTGAGTGAT+GGG + chr7_2:29989610-29989629 None:intergenic 40.0%
AAGTGAAGATTGGTGAGTGA+TGG + chr7_2:29989611-29989630 None:intergenic 40.0%
!! TTAAGAGTGTCACAACCTGA+AGG - chr7_2:29989884-29989903 Msa1055500:CDS 40.0%
TTATGGGAGAAGATTCCTTC+AGG + chr7_2:29989902-29989921 None:intergenic 40.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 29989475 29989980 29989475 ID=Msa1055500;Name=Msa1055500
chr7_2 mRNA 29989475 29989980 29989475 ID=Msa1055500-mRNA-1;Parent=Msa1055500;Name=Msa1055500-mRNA-1;_AED=0.45;_eAED=0.70;_QI=0|0|0|1|0|0|3|0|129
chr7_2 exon 29989771 29989980 29989771 ID=Msa1055500-mRNA-1:exon:6208;Parent=Msa1055500-mRNA-1
chr7_2 exon 29989579 29989666 29989579 ID=Msa1055500-mRNA-1:exon:6207;Parent=Msa1055500-mRNA-1
chr7_2 exon 29989475 29989566 29989475 ID=Msa1055500-mRNA-1:exon:6206;Parent=Msa1055500-mRNA-1
chr7_2 CDS 29989771 29989980 29989771 ID=Msa1055500-mRNA-1:cds;Parent=Msa1055500-mRNA-1
chr7_2 CDS 29989579 29989666 29989579 ID=Msa1055500-mRNA-1:cds;Parent=Msa1055500-mRNA-1
chr7_2 CDS 29989475 29989566 29989475 ID=Msa1055500-mRNA-1:cds;Parent=Msa1055500-mRNA-1
Gene Sequence

>Msa1055500

ATGATAACAATTACAAAGACATTCAAAGAAAAACTCACCAAAACATTTGTGCCTTCCACTTTCTATTCTCTGCTACCTTTAGCTCTAATTTGCCTATACTTTTACTTCTCCTCATTTACTCCATTTTACACTCCCATCACTCACCAATCTTCACTTTCTATTTCCCCTCCTTTAACAAAAGGTAAATTAACTAGCACTGTAACTTTGCTAAATTTGATCGATATTTTTGATGGGGGAACTTCTATGTACAATTCAAAGTTGTTTTTTGTTTGTGTCTCACTTTATAGATACTTTAGAAAGTGTCACAACCTGAAGGAATCTTCTCCCATAAAAGTTTTCTCCCGTGTAATCTCAACTATTGTCTTTTTTTTTGTACCACAAGCATTTTAA

Protein sequence

>Msa1055500

MITITKTFKEKLTKTFVPSTFYSLLPLALICLYFYFSSFTPFYTPITHQSSLSISPPLTKGKLTSTVTLLNLIDIFDGGTSMYNSKLFFVCVSLYRYFRKCHNLKESSPIKVFSRVISTIVFFFVPQAF*