AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1408340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408340 MtrunA17_Chr8g0388181 96.032 126 5 0 1 126 1 126 2.16e-83 239
Msa1408340 MtrunA17_Chr8g0388191 75.397 126 28 3 1 125 5 128 1.13e-59 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCATGCAGTTTGCTTT+TGG 0.234778 tig0023380:+59462 None:intergenic
GGACTCAGAACCAACCATTT+TGG 0.266732 tig0023380:-59417 Msa1408340:CDS
CGTGAGTTACTTTCTATTCA+AGG 0.359033 tig0023380:-59230 Msa1408340:CDS
TAGTAATGATTTAGTAGAAA+AGG 0.375647 tig0023380:-59348 Msa1408340:CDS
AAAGAAAAGGGTCCGGTTTG+TGG 0.426219 tig0023380:-59438 Msa1408340:CDS
CTGCATGCAAGACAAAGAAA+AGG 0.503875 tig0023380:-59451 Msa1408340:CDS
TAATGATTTAGTAGAAAAGG+AGG 0.512981 tig0023380:-59345 Msa1408340:CDS
GTGAGTTACTTTCTATTCAA+GGG 0.520035 tig0023380:-59229 Msa1408340:CDS
TGGAAGTGTTCTTGAATTGA+AGG 0.537857 tig0023380:-59261 Msa1408340:CDS
AATGATTTAGTAGAAAAGGA+GGG 0.545782 tig0023380:-59344 Msa1408340:CDS
TGCATGCAAGACAAAGAAAA+GGG 0.550595 tig0023380:-59450 Msa1408340:CDS
TTGGTTCTGAGTCCACAAAC+CGG 0.560281 tig0023380:+59426 None:intergenic
GCAAGACAAAGAAAAGGGTC+CGG 0.569438 tig0023380:-59445 Msa1408340:CDS
TTTGTACAAATGCTCCCTCA+CGG 0.598040 tig0023380:+59204 None:intergenic
TCTATTCAAGGGAACCGTGA+GGG 0.615406 tig0023380:-59218 Msa1408340:CDS
GAGTTGCTATCAAGATGCAA+TGG 0.620407 tig0023380:-59281 Msa1408340:CDS
AGTGACGATTAGATTAACAA+AGG 0.646753 tig0023380:-59315 Msa1408340:CDS
TTCTATTCAAGGGAACCGTG+AGG 0.687443 tig0023380:-59219 Msa1408340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTGATATTTTTTTA+AGG + tig0023380:59204-59223 None:intergenic 10.0%
!! TAGTAATGATTTAGTAGAAA+AGG - tig0023380:59390-59409 Msa1408340:CDS 20.0%
! TAATGATTTAGTAGAAAAGG+AGG - tig0023380:59393-59412 Msa1408340:CDS 25.0%
! AATGATTTAGTAGAAAAGGA+GGG - tig0023380:59394-59413 Msa1408340:CDS 25.0%
AGTGACGATTAGATTAACAA+AGG - tig0023380:59423-59442 Msa1408340:CDS 30.0%
! GTGAGTTACTTTCTATTCAA+GGG - tig0023380:59509-59528 Msa1408340:CDS 30.0%
TGCATGCAAGACAAAGAAAA+GGG - tig0023380:59288-59307 Msa1408340:CDS 35.0%
! TTTTCTTCTCCCAAAATGGT+TGG + tig0023380:59334-59353 None:intergenic 35.0%
!! GTTGTTTTCTTCTCCCAAAA+TGG + tig0023380:59338-59357 None:intergenic 35.0%
!! TGGAAGTGTTCTTGAATTGA+AGG - tig0023380:59477-59496 Msa1408340:CDS 35.0%
! CGTGAGTTACTTTCTATTCA+AGG - tig0023380:59508-59527 Msa1408340:CDS 35.0%
!! CTTGCATGCAGTTTGCTTTT+GGG + tig0023380:59278-59297 None:intergenic 40.0%
!! TCTTGCATGCAGTTTGCTTT+TGG + tig0023380:59279-59298 None:intergenic 40.0%
CTGCATGCAAGACAAAGAAA+AGG - tig0023380:59287-59306 Msa1408340:CDS 40.0%
! GACTCAGAACCAACCATTTT+GGG - tig0023380:59322-59341 Msa1408340:CDS 40.0%
GAGTTGCTATCAAGATGCAA+TGG - tig0023380:59457-59476 Msa1408340:CDS 40.0%
TTTGTACAAATGCTCCCTCA+CGG + tig0023380:59537-59556 None:intergenic 40.0%
GCAAGACAAAGAAAAGGGTC+CGG - tig0023380:59293-59312 Msa1408340:CDS 45.0%
! AAAGAAAAGGGTCCGGTTTG+TGG - tig0023380:59300-59319 Msa1408340:CDS 45.0%
!! TTGGTTCTGAGTCCACAAAC+CGG + tig0023380:59315-59334 None:intergenic 45.0%
! GGACTCAGAACCAACCATTT+TGG - tig0023380:59321-59340 Msa1408340:CDS 45.0%
TTCTATTCAAGGGAACCGTG+AGG - tig0023380:59519-59538 Msa1408340:CDS 45.0%
TCTATTCAAGGGAACCGTGA+GGG - tig0023380:59520-59539 Msa1408340:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0023380 gene 59190 59570 59190 ID=Msa1408340;Name=Msa1408340
tig0023380 mRNA 59190 59570 59190 ID=Msa1408340-mRNA-1;Parent=Msa1408340;Name=Msa1408340-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|126
tig0023380 exon 59190 59570 59190 ID=Msa1408340-mRNA-1:exon:25567;Parent=Msa1408340-mRNA-1
tig0023380 CDS 59190 59570 59190 ID=Msa1408340-mRNA-1:cds;Parent=Msa1408340-mRNA-1
Gene Sequence

>Msa1408340

ATGTTTGCTTGCCTTAAAAAAATATCACAAAAAAAGATAAAAGTAGAGCAAATTATCAAAGCAACAAAAGATGTATGTGAGTTAACCCAAAAGCAAACTGCATGCAAGACAAAGAAAAGGGTCCGGTTTGTGGACTCAGAACCAACCATTTTGGGAGAAGAAAACAACAATGAAGAATTTGAGAAAAGAAGATGTATTAGTAGTAATGATTTAGTAGAAAAGGAGGGAATTAGAGTGACGATTAGATTAACAAAGGAACAAGCAGCTGAGTTGCTATCAAGATGCAATGGAAGTGTTCTTGAATTGAAGGATGTTGCTCGTGAGTTACTTTCTATTCAAGGGAACCGTGAGGGAGCATTTGTACAAACAATTTCTAGTTAG

Protein sequence

>Msa1408340

MFACLKKISQKKIKVEQIIKATKDVCELTQKQTACKTKKRVRFVDSEPTILGEENNNEEFEKRRCISSNDLVEKEGIRVTIRLTKEQAAELLSRCNGSVLELKDVARELLSIQGNREGAFVQTISS*