AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0404190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404190 MtrunA17_Chr5g0412541 52.920 274 118 2 2 264 213 486 4.22e-102 306
Msa0404190 MtrunA17_Chr3g0132531 54.887 266 109 2 4 258 225 490 5.71e-98 295
Msa0404190 MtrunA17_Chr5g0412571 44.526 274 141 2 2 264 158 431 9.13e-79 244
Msa0404190 MtrunA17_Chr3g0132541 91.379 116 10 0 1 116 217 332 1.33e-69 221
Msa0404190 MtrunA17_Chr3g0132541 95.349 86 4 0 179 264 350 435 1.56e-49 168
Msa0404190 MtrunA17_Chr7g0254521 43.321 277 143 2 2 264 208 484 3.90e-69 221
Msa0404190 MtrunA17_Chr8g0368001 43.529 255 134 1 4 248 229 483 5.00e-69 221
Msa0404190 MtrunA17_Chr7g0254501 44.245 278 139 3 2 264 35 311 2.37e-68 214
Msa0404190 MtrunA17_Chr7g0254531 42.164 268 145 1 1 258 118 385 1.97e-66 211
Msa0404190 MtrunA17_Chr5g0412771 40.755 265 147 1 4 258 235 499 7.66e-66 213
Msa0404190 MtrunA17_Chr7g0254511 42.806 278 142 3 2 263 207 483 5.72e-65 210
Msa0404190 MtrunA17_Chr4g0024501 43.411 258 135 2 3 249 242 499 4.04e-64 209
Msa0404190 MtrunA17_Chr2g0322671 42.692 260 138 2 3 251 230 489 2.56e-63 206
Msa0404190 MtrunA17_Chr1g0154441 40.755 265 147 1 4 258 121 385 1.91e-62 201
Msa0404190 MtrunA17_Chr2g0288281 41.199 267 146 2 3 258 120 386 2.29e-62 201
Msa0404190 MtrunA17_Chr5g0442331 40.809 272 146 3 4 260 225 496 6.05e-62 202
Msa0404190 MtrunA17_Chr6g0460541 42.086 278 145 3 2 264 123 399 9.54e-61 197
Msa0404190 MtrunA17_Chr7g0248051 42.742 248 131 2 13 250 258 504 3.72e-58 193
Msa0404190 MtrunA17_Chr1g0202761 38.806 268 153 2 1 258 219 485 1.32e-56 188
Msa0404190 MtrunA17_Chr6g0468531 34.074 270 167 2 4 263 210 478 1.53e-53 180
Msa0404190 MtrunA17_Chr6g0468521 35.271 258 156 2 4 251 209 465 4.37e-52 176
Msa0404190 MtrunA17_Chr6g0468511 33.333 258 161 2 4 251 209 465 1.69e-41 148
Msa0404190 MtrunA17_Chr6g0460521 44.231 208 106 1 60 257 1 208 2.28e-39 136
Msa0404190 MtrunA17_Chr8g0392601 30.112 269 177 2 2 259 189 457 7.89e-39 141
Msa0404190 MtrunA17_Chr6g0477961 32.593 270 169 3 7 263 209 478 3.73e-37 136
Msa0404190 MtrunA17_Chr6g0483341 28.102 274 186 2 1 263 218 491 2.08e-34 129
Msa0404190 MtrunA17_Chr6g0483351 32.685 257 160 3 7 250 124 380 3.68e-34 127
Msa0404190 MtrunA17_Chr7g0251331 30.403 273 179 2 3 264 204 476 8.29e-34 127
Msa0404190 MtrunA17_Chr6g0483381 31.387 274 175 3 3 263 205 478 7.01e-33 125
Msa0404190 MtrunA17_Chr1g0208791 30.515 272 177 3 2 262 220 490 2.18e-32 124
Msa0404190 MtrunA17_Chr6g0483361 30.370 270 175 3 7 263 207 476 2.91e-32 123
Msa0404190 MtrunA17_Chr6g0483331 29.197 274 183 2 1 263 189 462 3.10e-32 123
Msa0404190 MtrunA17_Chr2g0317221 30.556 252 164 2 7 247 133 384 7.79e-32 121
Msa0404190 MtrunA17_Chr8g0364751 37.931 174 98 1 97 260 6 179 2.43e-31 114
Msa0404190 MtrunA17_Chr6g0449061 29.368 269 179 3 3 260 209 477 1.22e-30 119
Msa0404190 MtrunA17_Chr1g0208821 29.924 264 174 3 6 258 222 485 1.48e-30 119
Msa0404190 MtrunA17_Chr2g0317131 29.259 270 174 3 6 264 224 487 1.55e-30 119
Msa0404190 MtrunA17_Chr6g0483161 29.150 247 165 3 4 249 123 360 4.38e-29 113
Msa0404190 MtrunA17_Chr2g0317171 28.253 269 176 3 7 264 219 481 9.77e-29 114
Msa0404190 MtrunA17_Chr1g0208881 26.690 281 179 4 3 264 203 475 8.71e-28 110
Msa0404190 MtrunA17_Chr7g0251321 26.744 258 179 3 3 259 120 368 2.03e-27 108
Msa0404190 MtrunA17_Chr2g0317161 27.509 269 178 3 7 264 213 475 4.15e-27 109
Msa0404190 MtrunA17_Chr1g0208891 29.572 257 170 3 3 248 202 458 1.09e-26 107
Msa0404190 MtrunA17_Chr2g0317151 27.778 270 178 3 6 264 214 477 9.44e-26 105
Msa0404190 MtrunA17_Chr5g0440661 30.204 245 115 6 4 247 121 310 3.78e-25 101
Msa0404190 MtrunA17_Chr6g0487091 24.686 239 170 2 35 263 161 399 3.00e-22 95.1
Msa0404190 MtrunA17_Chr6g0467321 26.271 236 157 3 46 264 272 507 7.59e-20 88.6
Msa0404190 MtrunA17_Chr4g0013031 21.377 276 204 3 1 263 242 517 1.09e-19 88.2
Msa0404190 MtrunA17_Chr5g0428251 23.651 241 174 2 34 264 265 505 3.44e-19 86.7
Msa0404190 MtrunA17_Chr5g0397991 24.621 264 188 3 1 253 241 504 4.71e-19 86.3
Msa0404190 MtrunA17_Chr8g0389801 24.783 230 163 3 35 254 232 461 5.09e-18 83.2
Msa0404190 MtrunA17_Chr2g0295631 24.775 222 157 1 52 263 264 485 1.18e-17 82.0
Msa0404190 MtrunA17_Chr3g0140931 24.370 238 170 2 34 261 261 498 3.08e-17 80.9
Msa0404190 MtrunA17_Chr6g0483264 27.451 204 138 1 63 256 1 204 4.66e-17 77.8
Msa0404190 MtrunA17_Chr1g0203791 23.019 265 191 5 1 253 240 503 9.02e-16 76.6
Msa0404190 MtrunA17_Chr6g0473701 22.826 276 201 3 1 264 304 579 1.04e-15 76.6
Msa0404190 MtrunA17_Chr8g0364761 36.559 93 57 2 4 94 232 324 2.09e-14 72.0
Msa0404190 MtrunA17_Chr6g0483221 34.314 102 67 0 148 249 54 155 7.95e-14 67.8
Msa0404190 MtrunA17_Chr5g0398571 24.775 222 157 2 52 263 75 296 1.31e-12 66.6
Msa0404190 MtrunA17_Chr6g0483261 33.945 109 72 0 148 256 54 162 4.35e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404190 AT5G65380.1 50.929 269 121 2 1 258 212 480 7.21e-94 285
Msa0404190 AT5G10420.1 46.642 268 132 2 2 258 214 481 2.92e-81 253
Msa0404190 AT5G44050.1 45.255 274 139 2 1 263 215 488 4.37e-79 247
Msa0404190 AT3G26590.2 40.755 265 147 1 4 258 121 385 9.84e-69 218
Msa0404190 AT3G26590.1 40.755 265 147 1 4 258 223 487 7.01e-68 218
Msa0404190 AT1G33100.1 42.029 276 145 3 4 264 211 486 4.06e-67 216
Msa0404190 AT5G38030.1 41.132 265 146 1 4 258 223 487 1.43e-66 215
Msa0404190 AT3G21690.1 41.758 273 148 2 3 264 229 501 5.84e-66 213
Msa0404190 AT1G33110.1 42.578 256 137 1 4 249 214 469 5.88e-65 211
Msa0404190 AT1G12950.1 41.132 265 146 1 4 258 245 509 8.42e-65 211
Msa0404190 AT1G23300.1 41.573 267 146 1 2 258 220 486 1.24e-64 210
Msa0404190 AT1G61890.1 40.809 272 150 2 4 264 225 496 5.68e-63 206
Msa0404190 AT1G11670.1 40.590 271 148 3 4 261 228 498 5.68e-63 206
Msa0404190 AT3G59030.1 41.392 273 147 3 4 264 229 500 7.35e-63 205
Msa0404190 AT1G47530.2 40.377 265 148 1 4 258 121 385 2.13e-62 201
Msa0404190 AT1G61890.2 42.636 258 137 2 4 250 225 482 4.41e-62 203
Msa0404190 AT1G47530.1 40.530 264 147 1 4 257 213 476 1.14e-61 202
Msa0404190 AT1G33090.1 42.578 256 137 1 4 249 214 469 1.21e-61 202
Msa0404190 AT3G03620.2 41.199 267 147 1 1 257 212 478 3.46e-61 201
Msa0404190 AT3G03620.1 41.199 267 147 1 1 257 212 478 3.46e-61 201
Msa0404190 AT5G17700.2 39.326 267 152 1 1 257 209 475 7.75e-60 197
Msa0404190 AT5G17700.1 39.326 267 152 1 1 257 209 475 7.75e-60 197
Msa0404190 AT1G33080.1 41.473 258 141 1 4 251 214 471 9.63e-58 192
Msa0404190 AT1G33080.2 41.379 261 143 1 4 254 214 474 1.08e-57 191
Msa0404190 AT4G00350.1 38.491 265 152 2 4 258 266 529 7.79e-56 187
Msa0404190 AT4G21903.3 37.184 277 161 3 1 264 118 394 1.03e-53 179
Msa0404190 AT4G21903.1 37.184 277 161 3 1 264 227 503 1.34e-52 178
Msa0404190 AT4G21903.2 37.363 273 160 2 1 262 227 499 1.75e-52 178
Msa0404190 AT4G21910.3 37.455 275 159 3 1 262 229 503 2.08e-52 178
Msa0404190 AT4G21910.1 37.455 275 159 3 1 262 229 503 2.08e-52 178
Msa0404190 AT4G21910.2 37.455 275 159 3 1 262 231 505 2.32e-52 178
Msa0404190 AT4G21910.4 37.455 275 159 3 1 262 231 505 2.32e-52 178
Msa0404190 AT1G61890.3 41.818 220 117 2 4 212 225 444 2.36e-48 166
Msa0404190 AT4G25640.1 37.818 275 156 3 4 264 212 485 3.54e-47 164
Msa0404190 AT4G25640.2 37.818 275 156 3 4 264 212 485 6.11e-47 164
Msa0404190 AT1G33110.2 40.957 188 101 1 4 181 214 401 7.69e-40 142
Msa0404190 AT4G25640.3 38.053 226 129 2 4 219 212 436 7.02e-37 136
Msa0404190 AT5G52450.1 31.852 270 173 2 3 261 203 472 4.16e-34 129
Msa0404190 AT1G71140.1 30.483 269 174 4 3 259 203 470 1.02e-33 127
Msa0404190 AT1G66780.1 32.210 267 170 3 1 256 210 476 1.55e-33 127
Msa0404190 AT5G17700.3 36.788 193 112 1 1 183 209 401 2.02e-33 125
Msa0404190 AT3G59030.2 39.153 189 102 3 4 180 229 416 6.63e-33 124
Msa0404190 AT1G15170.2 31.179 263 168 3 3 253 92 353 1.53e-31 120
Msa0404190 AT1G15160.1 31.439 264 160 5 6 253 211 469 4.96e-31 120
Msa0404190 AT1G15170.1 31.179 263 168 3 3 253 211 472 5.19e-31 120
Msa0404190 AT1G15150.2 29.658 263 172 3 3 253 92 353 7.29e-31 118
Msa0404190 AT2G34360.1 32.014 278 162 7 3 264 204 470 9.30e-31 119
Msa0404190 AT1G15150.1 29.658 263 172 3 3 253 208 469 2.06e-30 119
Msa0404190 AT1G73700.1 33.212 274 170 4 3 263 201 474 7.14e-29 114
Msa0404190 AT3G23550.1 29.297 256 170 2 3 247 208 463 5.86e-28 111
Msa0404190 AT2G04070.1 29.008 262 175 3 3 253 204 465 5.91e-28 112
Msa0404190 AT1G64820.1 26.937 271 187 3 1 260 204 474 7.20e-28 111
Msa0404190 AT3G23560.1 28.906 256 171 2 3 247 216 471 7.85e-27 108
Msa0404190 AT2G04080.2 30.268 261 167 4 3 250 204 462 1.60e-26 107
Msa0404190 AT2G04100.1 28.413 271 183 2 3 262 207 477 2.87e-26 107
Msa0404190 AT2G04040.1 29.213 267 174 5 3 256 204 468 5.46e-26 106
Msa0404190 AT2G04090.1 28.253 269 182 2 3 260 207 475 5.78e-26 106
Msa0404190 AT2G04100.2 29.344 259 172 2 3 250 207 465 6.96e-26 105
Msa0404190 AT2G04080.1 28.839 267 175 4 3 256 204 468 7.49e-26 105
Msa0404190 AT2G34360.2 30.337 267 139 6 3 264 204 428 1.15e-25 105
Msa0404190 AT2G04050.1 29.008 262 175 3 3 253 204 465 2.60e-24 101
Msa0404190 AT1G66760.2 28.044 271 176 5 4 259 206 472 8.50e-24 100
Msa0404190 AT1G66760.3 27.626 257 175 3 4 249 78 334 1.59e-23 97.8
Msa0404190 AT1G66760.1 29.119 261 166 5 4 249 206 462 3.83e-23 98.2
Msa0404190 AT1G15180.1 28.244 262 177 2 3 253 212 473 3.63e-21 92.8
Msa0404190 AT2G38510.1 22.105 285 201 5 1 264 181 465 1.36e-19 88.2
Msa0404190 AT4G29140.1 23.273 275 200 3 1 264 231 505 6.20e-19 86.3
Msa0404190 AT1G71870.1 26.636 214 146 2 46 248 262 475 8.98e-18 82.8
Msa0404190 AT2G04066.1 36.697 109 69 0 148 256 55 163 3.70e-17 77.4
Msa0404190 AT1G58340.1 22.822 241 169 4 34 263 267 501 1.09e-16 79.7
Msa0404190 AT4G23030.1 22.541 244 174 4 23 253 230 471 2.24e-14 72.8
Msa0404190 AT5G19700.1 22.321 224 164 2 35 248 246 469 1.60e-13 70.5
Msa0404190 AT5G52050.1 23.792 269 188 4 3 254 212 480 9.93e-13 68.2
Msa0404190 AT3G23560.2 27.513 189 126 2 3 180 216 404 3.73e-12 66.2
Msa0404190 AT4G22790.1 24.454 229 153 5 52 263 258 483 1.28e-11 64.7

Find 132 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATTATAGCTTGGGTTTC+AGG 0.117752 3_2:-34956837 Msa0404190:five_prime_UTR
CTCTTGGTGGCTCTCAGTTT+TGG 0.133449 3_2:-34956724 Msa0404190:CDS
TGCATTCAACCTGTTCTTTC+AGG 0.142842 3_2:-34955036 Msa0404190:intron
TTGATTCCAAATATGTTTCT+TGG 0.216157 3_2:+34957379 None:intergenic
CTTGATCCAAGAAACATATT+TGG 0.219818 3_2:-34957385 Msa0404190:five_prime_UTR
TGATCGTTGGATTCATATTT+TGG 0.230229 3_2:-34955173 Msa0404190:CDS
GTGTTTATTTGCAACGTTCC+TGG 0.234851 3_2:-34957042 Msa0404190:five_prime_UTR
TTTAAACTATCTTTGGCTTC+TGG 0.239770 3_2:-34956615 Msa0404190:CDS
TGTCGTCCTTAACATGAATT+TGG 0.240625 3_2:+34954378 None:intergenic
GGATTTCTTTAAACTATCTT+TGG 0.246839 3_2:-34956622 Msa0404190:CDS
GTTGGCAGTGTAACTATTTA+TGG 0.262029 3_2:-34956308 Msa0404190:intron
CTTTGCAACGTGAATTGAAA+TGG 0.302343 3_2:+34956885 None:intergenic
CTCTTACCATTGATTTCTCT+TGG 0.315447 3_2:-34956740 Msa0404190:CDS
GTTGCGGTTTGGTTGCAATT+TGG 0.318064 3_2:-34956932 Msa0404190:five_prime_UTR
TTGATAGTTCATTGACCATT+TGG 0.318971 3_2:+34955088 None:intergenic
TGTTGCAATCGATGCTCTTT+CGG 0.328960 3_2:-34956431 Msa0404190:CDS
AGCCATCACTTTCGGCTTCT+TGG 0.329444 3_2:-34957217 Msa0404190:intron
TCATAAATCTGAATATTTAT+CGG 0.329762 3_2:+34954220 None:intergenic
TTGGTGGGTGTCCACATTCA+TGG 0.331369 3_2:-34956680 Msa0404190:CDS
CTCCTCATAGCCATCACTTT+CGG 0.346686 3_2:-34957225 Msa0404190:five_prime_UTR
ACTTGGTTGGAATACCTCTT+GGG 0.349339 3_2:-34954832 Msa0404190:CDS
TTATCTGTAAACACAGAAAC+TGG 0.351131 3_2:-34954191 Msa0404190:three_prime_UTR
TGGATCAAGGAGTTATTGTT+TGG 0.356520 3_2:+34957399 None:intergenic
TACTTGGTTGGAATACCTCT+TGG 0.358926 3_2:-34954833 Msa0404190:CDS
AGATGAGAGTTGGGATTGTT+GGG 0.367818 3_2:-34956767 Msa0404190:CDS
AAGATGAGAGTTGGGATTGT+TGG 0.377537 3_2:-34956768 Msa0404190:CDS
ATGTTTGTGTATACACTCTT+TGG 0.381883 3_2:-34956699 Msa0404190:CDS
CAAATATGTTTCTTGGATCA+AGG 0.387412 3_2:+34957386 None:intergenic
CAGTGTAACTATTTATGGTT+GGG 0.389493 3_2:-34956303 Msa0404190:CDS
CAATCGATGCTCTTTCGGTT+TGG 0.398004 3_2:-34956426 Msa0404190:intron
CGATTATAGCTTGGGTTTCA+GGG 0.400701 3_2:-34956836 Msa0404190:five_prime_UTR
ACCCTTTGTGGCCAAGCTTA+CGG 0.402093 3_2:-34957088 Msa0404190:five_prime_UTR
CTCTTGGGGTTCTCCTAGGC+TGG 0.407134 3_2:-34954817 Msa0404190:CDS
TCAGGCGTTGCAGTTGGATC+TGG 0.407708 3_2:-34954896 Msa0404190:intron
GAGGGTGCAGGCAATGGAAA+TGG 0.408586 3_2:+34957245 None:intergenic
GACTCATGTGTTAGTGAGTT+GGG 0.409020 3_2:-34956802 Msa0404190:five_prime_UTR
AATGGGAAACTCAAATGAAA+TGG 0.411803 3_2:+34956903 None:intergenic
GAAAACTGCGATTATAGCTT+GGG 0.412773 3_2:-34956844 Msa0404190:five_prime_UTR
ATTGCCTTGCGCCGTAAGCT+TGG 0.413205 3_2:+34957077 None:intergenic
CTTGGCGCAGGCATGCAAAA+GGG 0.413355 3_2:-34955233 Msa0404190:intron
TTATTATGTTGAGATACAAT+TGG 0.423408 3_2:-34954525 Msa0404190:CDS
TTGTTTATAAGATGAGAGTT+GGG 0.435990 3_2:-34956776 Msa0404190:exon
TATTATGTTGAGATACAATT+GGG 0.436889 3_2:-34954524 Msa0404190:CDS
TGTTTATTTGCAACGTTCCT+GGG 0.440254 3_2:-34957041 Msa0404190:five_prime_UTR
GAATGTCCAAATTCATGTTA+AGG 0.444233 3_2:-34954384 Msa0404190:CDS
TTTGTTTATAAGATGAGAGT+TGG 0.450693 3_2:-34956777 Msa0404190:exon
ATTGTATCTCAACATAATAA+TGG 0.452420 3_2:+34954528 None:intergenic
ATTTATCATATCAGGGTATG+TGG 0.452640 3_2:-34954594 Msa0404190:intron
CAATGAACTATCAATCTTGT+TGG 0.455689 3_2:-34955079 Msa0404190:CDS
AACCTGTGGCTGTTGCGGTT+TGG 0.455898 3_2:-34956943 Msa0404190:five_prime_UTR
TTTGCAACGTGAATTGAAAT+GGG 0.456859 3_2:+34956886 None:intergenic
TGACTCATGTGTTAGTGAGT+TGG 0.458522 3_2:-34956803 Msa0404190:five_prime_UTR
TTACCATTGATTTCTCTTGG+TGG 0.460239 3_2:-34956737 Msa0404190:CDS
TGAAAACTGCGATTATAGCT+TGG 0.460819 3_2:-34956845 Msa0404190:five_prime_UTR
GTTTATTCTGTAGCTTGGAA+TGG 0.464732 3_2:-34957125 Msa0404190:intron
ATACCTCTTGGGGTTCTCCT+AGG 0.469811 3_2:-34954821 Msa0404190:CDS
TTCTCTGTTTCAGATTCACT+TGG 0.470202 3_2:+34957459 None:intergenic
AGTGCTCTTGAGACCCTTTG+TGG 0.471299 3_2:-34957100 Msa0404190:five_prime_UTR
GCTTGGCGCAGGCATGCAAA+AGG 0.472766 3_2:-34955234 Msa0404190:intron
ACGCCTATGTTGAAGTTCAT+AGG 0.473055 3_2:-34956968 Msa0404190:five_prime_UTR
AGAGATTTACCTGAAAGAAC+AGG 0.473904 3_2:+34955027 None:intergenic
GTGGTAAATACTGTGATCGT+TGG 0.477830 3_2:-34955186 Msa0404190:CDS
CTAGGCTGGCTGCTACCTTC+CGG 0.483061 3_2:-34954803 Msa0404190:CDS
GCAGTGTAACTATTTATGGT+TGG 0.486919 3_2:-34956304 Msa0404190:intron
ATTGGTAGTTACTACTTGGT+TGG 0.489915 3_2:-34954845 Msa0404190:CDS
AAGCTTATCTGTAATCCAAA+TGG 0.491533 3_2:-34955103 Msa0404190:CDS
CGCCGTAAGCTTGGCCACAA+AGG 0.497033 3_2:+34957086 None:intergenic
AAACATTGGTAGTTACTACT+TGG 0.497420 3_2:-34954849 Msa0404190:CDS
GGTGGTTCAAACATCGATCT+TGG 0.498189 3_2:-34954551 Msa0404190:CDS
CATGGTAAGACGGCTGAAGA+TGG 0.500552 3_2:+34957329 None:intergenic
GCCGTAAGCTTGGCCACAAA+GGG 0.503454 3_2:+34957087 None:intergenic
GTCGAGATCGTTGAGGTGGC+CGG 0.508489 3_2:+34957275 None:intergenic
CGGAAGGTAGCAGCCAGCCT+AGG 0.510636 3_2:+34954804 None:intergenic
GTGGCCAAGCTTACGGCGCA+AGG 0.520934 3_2:-34957081 Msa0404190:five_prime_UTR
AGGACAACCTGTGGCTGTTG+CGG 0.522079 3_2:-34956948 Msa0404190:five_prime_UTR
GCAAGGCAATACCACATGTT+GGG 0.522375 3_2:-34957064 Msa0404190:five_prime_UTR
CGCAAGGCAATACCACATGT+TGG 0.523921 3_2:-34957065 Msa0404190:five_prime_UTR
TTTGTGTATACACTCTTTGG+TGG 0.528257 3_2:-34956696 Msa0404190:CDS
GATGGCTATGAGGAGGGTGC+AGG 0.530107 3_2:+34957233 None:intergenic
TCATATCAGGGTATGTGGAC+TGG 0.531300 3_2:-34954589 Msa0404190:intron
TGTTGAGGTGGTCTTGTAAG+AGG 0.537361 3_2:+34954260 None:intergenic
TTGTTTGGTTGAAGTTGTGA+TGG 0.537615 3_2:+34957414 None:intergenic
ATTCGCGACGGTGGTTTCAG+TGG 0.538051 3_2:-34955205 Msa0404190:CDS
TGGGAATCCATGATACCGCT+TGG 0.540935 3_2:-34956284 Msa0404190:CDS
TCTCTGTTTCAGATTCACTT+GGG 0.542946 3_2:+34957460 None:intergenic
TATGAGGAGGGTGCAGGCAA+TGG 0.546306 3_2:+34957239 None:intergenic
GGGTGTCCACATTCATGGAA+TGG 0.547907 3_2:-34956675 Msa0404190:CDS
CTTGGTTGGAATACCTCTTG+GGG 0.549836 3_2:-34954831 Msa0404190:CDS
GAGATACAATTGGGAAAGAG+AGG 0.551696 3_2:-34954515 Msa0404190:intron
AGATTGAGAGACGACGGTGA+TGG 0.553015 3_2:+34957302 None:intergenic
TGTCCTATGAACTTCAACAT+AGG 0.557874 3_2:+34956965 None:intergenic
GTAAGACGGCTGAAGATGGA+AGG 0.562521 3_2:+34957333 None:intergenic
GATTATAGCTTGGGTTTCAG+GGG 0.564961 3_2:-34956835 Msa0404190:five_prime_UTR
AAGAATTACAATCTGTGTTG+AGG 0.572761 3_2:+34954245 None:intergenic
ATCTGGTCGTCAAGCAGTAG+TGG 0.575050 3_2:-34954879 Msa0404190:CDS
TTGGCGCAGGCATGCAAAAG+GGG 0.577017 3_2:-34955232 Msa0404190:intron
AGCCGAAAGTGATGGCTATG+AGG 0.577505 3_2:+34957223 None:intergenic
TTGTGTATACACTCTTTGGT+GGG 0.581821 3_2:-34956695 Msa0404190:CDS
GTCGTCTCTCAATCTTTCGC+CGG 0.585098 3_2:-34957294 Msa0404190:five_prime_UTR
AACCAAACCGCAACAGCCAC+AGG 0.595299 3_2:+34956941 None:intergenic
GAGCCACCAAGAGAAATCAA+TGG 0.597348 3_2:+34956734 None:intergenic
GCAAATAAACACCCAACATG+TGG 0.601982 3_2:+34957053 None:intergenic
CGAAAGTGATGGCTATGAGG+AGG 0.604744 3_2:+34957226 None:intergenic
AATTACAATCTGTGTTGAGG+TGG 0.606978 3_2:+34954248 None:intergenic
GCTGCTACCTTCCGGCATTG+TGG 0.608816 3_2:-34954795 Msa0404190:intron
AAGACGGCTGAAGATGGAAG+GGG 0.608995 3_2:+34957335 None:intergenic
TACCAAGAAGCCGAAAGTGA+TGG 0.609759 3_2:+34957215 None:intergenic
ATTATAGCTTGGGTTTCAGG+GGG 0.613902 3_2:-34956834 Msa0404190:five_prime_UTR
GATGACACTTACCACAATGC+CGG 0.618306 3_2:+34954784 None:intergenic
GAAAGTGATGGCTATGAGGA+GGG 0.620860 3_2:+34957227 None:intergenic
TAAGACGGCTGAAGATGGAA+GGG 0.626531 3_2:+34957334 None:intergenic
GGTGCAGGCAATGGAAATGG+CGG 0.628183 3_2:+34957248 None:intergenic
GTAGTGGCTTATATAAACAT+TGG 0.629710 3_2:-34954863 Msa0404190:CDS
GACGACGGTGATGGAGAACA+TGG 0.630629 3_2:+34957311 None:intergenic
AGGGGCGAAATTCGCGACGG+TGG 0.632818 3_2:-34955214 Msa0404190:CDS
TGATGGAGAACATGGTAAGA+CGG 0.640754 3_2:+34957319 None:intergenic
TGGAATGATGAGCGGAACGG+TGG 0.644281 3_2:-34954569 Msa0404190:CDS
CGAGGTCGAGATCGTTGAGG+TGG 0.650597 3_2:+34957271 None:intergenic
AGATGGAAGGGGCAGCAATG+TGG 0.663125 3_2:+34957346 None:intergenic
GGCGAAAGATTGAGAGACGA+CGG 0.666704 3_2:+34957296 None:intergenic
ACACTTACCACAATGCCGGA+AGG 0.670662 3_2:+34954788 None:intergenic
GGCGTGGTGAACACAAACAT+AGG 0.677344 3_2:+34956986 None:intergenic
CAGCCTAGGAGAACCCCAAG+AGG 0.678282 3_2:+34954818 None:intergenic
TATGAACTTCAACATAGGCG+TGG 0.680659 3_2:+34956970 None:intergenic
AGGCAATGGAAATGGCGGCG+AGG 0.691047 3_2:+34957253 None:intergenic
CGGCGAGGTCGAGATCGTTG+AGG 0.691600 3_2:+34957268 None:intergenic
AGACAAGAACAGAACAACCC+AGG 0.694782 3_2:+34957024 None:intergenic
GATGGCGTTGACTGTATCAG+TGG 0.699629 3_2:+34957432 None:intergenic
GACTGGAATGATGAGCGGAA+CGG 0.706808 3_2:-34954572 Msa0404190:CDS
ATGTGGACTGGAATGATGAG+CGG 0.720954 3_2:-34954577 Msa0404190:CDS
AAAAGGGGCGAAATTCGCGA+CGG 0.730071 3_2:-34955217 Msa0404190:CDS
GAAGTTCATAGGACAACCTG+TGG 0.734747 3_2:-34956957 Msa0404190:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAAACTAAAAAGAAA+AGG + chr3_2:34955225-34955244 None:intergenic 10.0%
!!! AATTTAAAACCATTAATTAA+AGG - chr3_2:34955975-34955994 Msa0404190:intron 10.0%
!!! CTGATTTTAACTTATTTTTT+AGG - chr3_2:34955054-34955073 Msa0404190:CDS 15.0%
!! CTTTATATACTGATAAAATA+CGG - chr3_2:34955871-34955890 Msa0404190:intron 15.0%
!! TTTATATACTGATAAAATAC+GGG - chr3_2:34955872-34955891 Msa0404190:intron 15.0%
!!! TAATTAATGGTTTTAAATTG+TGG + chr3_2:34955974-34955993 None:intergenic 15.0%
!! ATTGTGAAATGATTAAAAAA+GGG + chr3_2:34956328-34956347 None:intergenic 15.0%
!! TATCTTTACAAATATTTATG+TGG + chr3_2:34956959-34956978 None:intergenic 15.0%
!! ATGATTTATTTATCATATCA+GGG - chr3_2:34957010-34957029 Msa0404190:five_prime_UTR 15.0%
!!! ATGAATTTACCTTTTTTTAT+TGG + chr3_2:34957269-34957288 None:intergenic 15.0%
!! TCATAAATCTGAATATTTAT+CGG + chr3_2:34957394-34957413 None:intergenic 15.0%
!! CACTGTATTTAACTATTTAT+TGG - chr3_2:34955261-34955280 Msa0404190:intron 20.0%
!!! CTTCCTAATTTAATGATTTT+TGG - chr3_2:34955412-34955431 Msa0404190:intron 20.0%
!! CTACCAAAAATCATTAAATT+AGG + chr3_2:34955418-34955437 None:intergenic 20.0%
!! AAGATATCAATAGAAGAAAA+AGG + chr3_2:34956264-34956283 None:intergenic 20.0%
!! TTGTGAAATGATTAAAAAAG+GGG + chr3_2:34956327-34956346 None:intergenic 20.0%
!! CATTGTGAAATGATTAAAAA+AGG + chr3_2:34956329-34956348 None:intergenic 20.0%
!! CGTAAGATAAATAAAGTTTA+AGG - chr3_2:34956877-34956896 Msa0404190:five_prime_UTR 20.0%
!! GATGATTTATTTATCATATC+AGG - chr3_2:34957009-34957028 Msa0404190:five_prime_UTR 20.0%
!! ATTGTATCTCAACATAATAA+TGG + chr3_2:34957086-34957105 None:intergenic 20.0%
!! TTATTATGTTGAGATACAAT+TGG - chr3_2:34957086-34957105 Msa0404190:five_prime_UTR 20.0%
!! TATTATGTTGAGATACAATT+GGG - chr3_2:34957087-34957106 Msa0404190:five_prime_UTR 20.0%
!!! ATTTACCTTTTTTTATTGGT+TGG + chr3_2:34957265-34957284 None:intergenic 20.0%
! TTGATTCCAAATATGTTTCT+TGG + chr3_2:34954235-34954254 None:intergenic 25.0%
! TACAGAATAAACAAAGAGTA+AGG + chr3_2:34954478-34954497 None:intergenic 25.0%
! TTTGTTTATAAGATGAGAGT+TGG - chr3_2:34954834-34954853 Msa0404190:CDS 25.0%
! TTGTTTATAAGATGAGAGTT+GGG - chr3_2:34954835-34954854 Msa0404190:CDS 25.0%
! GGATTTCTTTAAACTATCTT+TGG - chr3_2:34954989-34955008 Msa0404190:intron 25.0%
! GTTAAAATCAGCACAAAAAA+AGG + chr3_2:34955045-34955064 None:intergenic 25.0%
!!! TTTTTGCTGAATTTTGTTGT+TGG - chr3_2:34955285-34955304 Msa0404190:intron 25.0%
! GATTTGAGAGGAATAAATAA+AGG + chr3_2:34955393-34955412 None:intergenic 25.0%
! TTAAATTAGGAAGATTTGAG+AGG + chr3_2:34955405-34955424 None:intergenic 25.0%
!!! TAGCATTGATTCCATTTAAT+GGG + chr3_2:34955464-34955483 None:intergenic 25.0%
!!! TTAGCATTGATTCCATTTAA+TGG + chr3_2:34955465-34955484 None:intergenic 25.0%
! GTTACATACTATTAAGCAAA+AGG + chr3_2:34955682-34955701 None:intergenic 25.0%
! AAATATATGTCACAGGATAA+TGG - chr3_2:34955816-34955835 Msa0404190:intron 25.0%
!!! TTGAGAGAGTTATTTTTGTT+GGG + chr3_2:34956293-34956312 None:intergenic 25.0%
!!! TTTGAGAGAGTTATTTTTGT+TGG + chr3_2:34956294-34956313 None:intergenic 25.0%
!!! CATATTTTGGTTGATAATTG+TGG - chr3_2:34956451-34956470 Msa0404190:CDS 25.0%
! TTATTTATCTTACGCAAGAA+TGG + chr3_2:34956871-34956890 None:intergenic 25.0%
CAAATATGTTTCTTGGATCA+AGG + chr3_2:34954228-34954247 None:intergenic 30.0%
CTTGATCCAAGAAACATATT+TGG - chr3_2:34954226-34954245 Msa0404190:three_prime_UTR 30.0%
! TCTTTGTTTATTCTGTAGCT+TGG - chr3_2:34954481-34954500 Msa0404190:intron 30.0%
AATGGGAAACTCAAATGAAA+TGG + chr3_2:34954711-34954730 None:intergenic 30.0%
TTTGCAACGTGAATTGAAAT+GGG + chr3_2:34954728-34954747 None:intergenic 30.0%
ATGTTTGTGTATACACTCTT+TGG - chr3_2:34954912-34954931 Msa0404190:intron 30.0%
! TTTAAACTATCTTTGGCTTC+TGG - chr3_2:34954996-34955015 Msa0404190:intron 30.0%
TTGTGTTTATGTGATATTCC+AGG - chr3_2:34955098-34955117 Msa0404190:CDS 30.0%
ATAATAGAAGTTCTCTAACC+TGG + chr3_2:34955119-34955138 None:intergenic 30.0%
CAGTGTAACTATTTATGGTT+GGG - chr3_2:34955308-34955327 Msa0404190:intron 30.0%
TTGAGAGGAATAAATAAAGG+AGG + chr3_2:34955390-34955409 None:intergenic 30.0%
!! TGGTTGTTTACTGATTTACA+GGG + chr3_2:34955497-34955516 None:intergenic 30.0%
!! ATGGTTGTTTACTGATTTAC+AGG + chr3_2:34955498-34955517 None:intergenic 30.0%
! TGATGCATTAGTTTATCTCT+TGG - chr3_2:34955626-34955645 Msa0404190:intron 30.0%
ATGTAGCAAATATATGTCAC+AGG - chr3_2:34955809-34955828 Msa0404190:intron 30.0%
TAATAAAGTCCGCAATATTG+CGG - chr3_2:34955948-34955967 Msa0404190:intron 30.0%
TCCATAAATCAAATGCCTTA+AGG - chr3_2:34956084-34956103 Msa0404190:intron 30.0%
! ACCTTAAGGCATTTGATTTA+TGG + chr3_2:34956088-34956107 None:intergenic 30.0%
AACATTATGTATACTAGAGC+TGG + chr3_2:34956210-34956229 None:intergenic 30.0%
AGAAAAAGGATCTATCACTT+TGG + chr3_2:34956250-34956269 None:intergenic 30.0%
ATTTCACAATGTCCATATCA+GGG - chr3_2:34956338-34956357 Msa0404190:intron 30.0%
! TGATCGTTGGATTCATATTT+TGG - chr3_2:34956438-34956457 Msa0404190:CDS 30.0%
AAGCTTATCTGTAATCCAAA+TGG - chr3_2:34956508-34956527 Msa0404190:intron 30.0%
! TTGATAGTTCATTGACCATT+TGG + chr3_2:34956526-34956545 None:intergenic 30.0%
CAATGAACTATCAATCTTGT+TGG - chr3_2:34956532-34956551 Msa0404190:intron 30.0%
TAAGTTCAGAAAACTGACAT+TGG + chr3_2:34956641-34956660 None:intergenic 30.0%
GTAGTGGCTTATATAAACAT+TGG - chr3_2:34956748-34956767 Msa0404190:CDS 30.0%
! AAACATTGGTAGTTACTACT+TGG - chr3_2:34956762-34956781 Msa0404190:CDS 30.0%
ATTTATCATATCAGGGTATG+TGG - chr3_2:34957017-34957036 Msa0404190:five_prime_UTR 30.0%
! GATAACATAACTTGTAACAG+TGG - chr3_2:34957176-34957195 Msa0404190:intron 30.0%
! TGTTATCTTGTTTGTAGGTA+CGG - chr3_2:34957205-34957224 Msa0404190:intron 30.0%
GAATGTCCAAATTCATGTTA+AGG - chr3_2:34957227-34957246 Msa0404190:five_prime_UTR 30.0%
AACGTCCAACCAATAAAAAA+AGG - chr3_2:34957257-34957276 Msa0404190:five_prime_UTR 30.0%
AAGAATTACAATCTGTGTTG+AGG + chr3_2:34957369-34957388 None:intergenic 30.0%
TTATCTGTAAACACAGAAAC+TGG - chr3_2:34957420-34957439 Msa0404190:five_prime_UTR 30.0%
TCTCTGTTTCAGATTCACTT+GGG + chr3_2:34954154-34954173 None:intergenic 35.0%
TTCTCTGTTTCAGATTCACT+TGG + chr3_2:34954155-34954174 None:intergenic 35.0%
TTGTTTGGTTGAAGTTGTGA+TGG + chr3_2:34954200-34954219 None:intergenic 35.0%
TGGATCAAGGAGTTATTGTT+TGG + chr3_2:34954215-34954234 None:intergenic 35.0%
GTTTATTCTGTAGCTTGGAA+TGG - chr3_2:34954486-34954505 Msa0404190:intron 35.0%
TGTTTATTTGCAACGTTCCT+GGG - chr3_2:34954570-34954589 Msa0404190:CDS 35.0%
TGTCCTATGAACTTCAACAT+AGG + chr3_2:34954649-34954668 None:intergenic 35.0%
CTTTGCAACGTGAATTGAAA+TGG + chr3_2:34954729-34954748 None:intergenic 35.0%
TGAAAACTGCGATTATAGCT+TGG - chr3_2:34954766-34954785 Msa0404190:intron 35.0%
GAAAACTGCGATTATAGCTT+GGG - chr3_2:34954767-34954786 Msa0404190:intron 35.0%
!! CTCTTACCATTGATTTCTCT+TGG - chr3_2:34954871-34954890 Msa0404190:CDS 35.0%
!! TTACCATTGATTTCTCTTGG+TGG - chr3_2:34954874-34954893 Msa0404190:CDS 35.0%
TTTGTGTATACACTCTTTGG+TGG - chr3_2:34954915-34954934 Msa0404190:intron 35.0%
TTGTGTATACACTCTTTGGT+GGG - chr3_2:34954916-34954935 Msa0404190:intron 35.0%
GAAAAACCATTCCATGAATG+TGG + chr3_2:34954945-34954964 None:intergenic 35.0%
! TTTTCAGCTGAAGCTTTTGT+TGG - chr3_2:34954960-34954979 Msa0404190:intron 35.0%
!!! TGAAGCTTTTGTTGGTTTGT+GGG - chr3_2:34954968-34954987 Msa0404190:intron 35.0%
GTTGGCAGTGTAACTATTTA+TGG - chr3_2:34955303-34955322 Msa0404190:intron 35.0%
GCAGTGTAACTATTTATGGT+TGG - chr3_2:34955307-34955326 Msa0404190:intron 35.0%
TCTCTCATGTACCCATTAAA+TGG - chr3_2:34955450-34955469 Msa0404190:intron 35.0%
AGGTGTATAGACATGTGATA+CGG + chr3_2:34955662-34955681 None:intergenic 35.0%
AATGCTTAGTGAGAACTGAT+TGG + chr3_2:34955779-34955798 None:intergenic 35.0%
!! AGTTCTCACTAAGCATTGAA+TGG - chr3_2:34955782-34955801 Msa0404190:intron 35.0%
CAAGAGCGACCTTTAATTAA+TGG + chr3_2:34955987-34956006 None:intergenic 35.0%
TCAACTCTTTCATCTAATGC+GGG + chr3_2:34956038-34956057 None:intergenic 35.0%
CTCAACTCTTTCATCTAATG+CGG + chr3_2:34956039-34956058 None:intergenic 35.0%
ATTAGATGAAAGAGTTGAGG+TGG - chr3_2:34956040-34956059 Msa0404190:intron 35.0%
CATCTTCACTCAAAACCTTA+AGG + chr3_2:34956102-34956121 None:intergenic 35.0%
!!! TAAGGTTTTGAGTGAAGATG+TGG - chr3_2:34956102-34956121 Msa0404190:intron 35.0%
CATTTCACAATGTCCATATC+AGG - chr3_2:34956337-34956356 Msa0404190:intron 35.0%
AGAGATTTACCTGAAAGAAC+AGG + chr3_2:34956587-34956606 None:intergenic 35.0%
! TACTTACCGATTGTATCTGT+TGG - chr3_2:34956682-34956701 Msa0404190:CDS 35.0%
CGAAAACCAACAGATACAAT+CGG + chr3_2:34956691-34956710 None:intergenic 35.0%
!!! TCTGTTGGTTTTCGATTTTC+AGG - chr3_2:34956697-34956716 Msa0404190:CDS 35.0%
! ATTGGTAGTTACTACTTGGT+TGG - chr3_2:34956766-34956785 Msa0404190:CDS 35.0%
!! GTGCTTGTTATCTTGTTTGT+AGG - chr3_2:34957200-34957219 Msa0404190:intron 35.0%
TGTCGTCCTTAACATGAATT+TGG + chr3_2:34957236-34957255 None:intergenic 35.0%
AATTACAATCTGTGTTGAGG+TGG + chr3_2:34957366-34957385 None:intergenic 35.0%
TGATGGAGAACATGGTAAGA+CGG + chr3_2:34954295-34954314 None:intergenic 40.0%
GCTTCTAGCATAAGCACTAT+TGG + chr3_2:34954426-34954445 None:intergenic 40.0%
ACAAAGAGTAAGGCTCTGTT+TGG + chr3_2:34954468-34954487 None:intergenic 40.0%
GCAAATAAACACCCAACATG+TGG + chr3_2:34954561-34954580 None:intergenic 40.0%
GTGTTTATTTGCAACGTTCC+TGG - chr3_2:34954569-34954588 Msa0404190:CDS 40.0%
TATGAACTTCAACATAGGCG+TGG + chr3_2:34954644-34954663 None:intergenic 40.0%
ACGCCTATGTTGAAGTTCAT+AGG - chr3_2:34954643-34954662 Msa0404190:intron 40.0%
CGATTATAGCTTGGGTTTCA+GGG - chr3_2:34954775-34954794 Msa0404190:intron 40.0%
GATTATAGCTTGGGTTTCAG+GGG - chr3_2:34954776-34954795 Msa0404190:intron 40.0%
ATTATAGCTTGGGTTTCAGG+GGG - chr3_2:34954777-34954796 Msa0404190:intron 40.0%
TGACTCATGTGTTAGTGAGT+TGG - chr3_2:34954808-34954827 Msa0404190:CDS 40.0%
GACTCATGTGTTAGTGAGTT+GGG - chr3_2:34954809-34954828 Msa0404190:CDS 40.0%
AAGATGAGAGTTGGGATTGT+TGG - chr3_2:34954843-34954862 Msa0404190:CDS 40.0%
AGATGAGAGTTGGGATTGTT+GGG - chr3_2:34954844-34954863 Msa0404190:CDS 40.0%
!!! CTGAAGCTTTTGTTGGTTTG+TGG - chr3_2:34954967-34954986 Msa0404190:intron 40.0%
TGTTGCAATCGATGCTCTTT+CGG - chr3_2:34955180-34955199 Msa0404190:CDS 40.0%
CAAAAAACCAAGCGGTATCA+TGG + chr3_2:34955337-34955356 None:intergenic 40.0%
!!! CATGATACCGCTTGGTTTTT+TGG - chr3_2:34955335-34955354 Msa0404190:intron 40.0%
CTTACAACCAGAAACATACC+CGG + chr3_2:34955367-34955386 None:intergenic 40.0%
CTGCGTGTTTATTCCATTTG+TGG - chr3_2:34955704-34955723 Msa0404190:intron 40.0%
AGTGAGAACTGATTGGCAAA+TGG + chr3_2:34955772-34955791 None:intergenic 40.0%
ACGGGAAAATGTGTCTAATG+CGG - chr3_2:34955890-34955909 Msa0404190:intron 40.0%
AAATTGTGGCCGCAATATTG+CGG + chr3_2:34955960-34955979 None:intergenic 40.0%
CGCATTAGATGAAAGAGTTG+AGG - chr3_2:34956037-34956056 Msa0404190:intron 40.0%
TATACTAGAGCTGGAGTTGT+TGG + chr3_2:34956201-34956220 None:intergenic 40.0%
TCACTTTGGCACGCTTATAT+AGG + chr3_2:34956236-34956255 None:intergenic 40.0%
GTGGTAAATACTGTGATCGT+TGG - chr3_2:34956425-34956444 Msa0404190:intron 40.0%
TGCATTCAACCTGTTCTTTC+AGG - chr3_2:34956575-34956594 Msa0404190:intron 40.0%
TACTTGGTTGGAATACCTCT+TGG - chr3_2:34956778-34956797 Msa0404190:exon 40.0%
ACTTGGTTGGAATACCTCTT+GGG - chr3_2:34956779-34956798 Msa0404190:exon 40.0%
TATCTTACGCAAGAATGGAG+TGG + chr3_2:34956866-34956885 None:intergenic 40.0%
AATGCAGATGATGTGTCTGA+CGG - chr3_2:34956985-34957004 Msa0404190:five_prime_UTR 40.0%
GAGATACAATTGGGAAAGAG+AGG - chr3_2:34957096-34957115 Msa0404190:five_prime_UTR 40.0%
ACAATTGGGAAAGAGAGGTA+AGG - chr3_2:34957101-34957120 Msa0404190:five_prime_UTR 40.0%
TAAGACGGCTGAAGATGGAA+GGG + chr3_2:34954280-34954299 None:intergenic 45.0%
GAAAGTGATGGCTATGAGGA+GGG + chr3_2:34954387-34954406 None:intergenic 45.0%
CTCCTCATAGCCATCACTTT+CGG - chr3_2:34954386-34954405 Msa0404190:CDS 45.0%
TACCAAGAAGCCGAAAGTGA+TGG + chr3_2:34954399-34954418 None:intergenic 45.0%
GCAAGGCAATACCACATGTT+GGG - chr3_2:34954547-34954566 Msa0404190:CDS 45.0%
AGACAAGAACAGAACAACCC+AGG + chr3_2:34954590-34954609 None:intergenic 45.0%
GAAGTTCATAGGACAACCTG+TGG - chr3_2:34954654-34954673 Msa0404190:intron 45.0%
!! GTTGCGGTTTGGTTGCAATT+TGG - chr3_2:34954679-34954698 Msa0404190:intron 45.0%
GCGATTATAGCTTGGGTTTC+AGG - chr3_2:34954774-34954793 Msa0404190:intron 45.0%
GAGCCACCAAGAGAAATCAA+TGG + chr3_2:34954880-34954899 None:intergenic 45.0%
!! TCTTGGTGGCTCTCAGTTTT+GGG - chr3_2:34954888-34954907 Msa0404190:CDS 45.0%
CAATCGATGCTCTTTCGGTT+TGG - chr3_2:34955185-34955204 Msa0404190:CDS 45.0%
TCAGTAAACAACCATCCCTG+TGG - chr3_2:34955503-34955522 Msa0404190:intron 45.0%
CAGTAAACAACCATCCCTGT+GGG - chr3_2:34955504-34955523 Msa0404190:intron 45.0%
AGTAAACAACCATCCCTGTG+GGG - chr3_2:34955505-34955524 Msa0404190:intron 45.0%
ATTAAAAGACCCCACAGGGA+TGG + chr3_2:34955517-34955536 None:intergenic 45.0%
CTCGATTAAAAGACCCCACA+GGG + chr3_2:34955521-34955540 None:intergenic 45.0%
ACTCGATTAAAAGACCCCAC+AGG + chr3_2:34955522-34955541 None:intergenic 45.0%
! CTGTGTTACTGCACCACAAA+TGG + chr3_2:34955720-34955739 None:intergenic 45.0%
TGGTGTCCAACTCACTTGTA+TGG - chr3_2:34956122-34956141 Msa0404190:intron 45.0%
GAGCAACCATACAAGTGAGT+TGG + chr3_2:34956131-34956150 None:intergenic 45.0%
CTTGGTTGGAATACCTCTTG+GGG - chr3_2:34956780-34956799 Msa0404190:exon 45.0%
GATGACACTTACCACAATGC+CGG + chr3_2:34956830-34956849 None:intergenic 45.0%
TCATATCAGGGTATGTGGAC+TGG - chr3_2:34957022-34957041 Msa0404190:five_prime_UTR 45.0%
!! ATGTGGACTGGAATGATGAG+CGG - chr3_2:34957034-34957053 Msa0404190:five_prime_UTR 45.0%
GGTGGTTCAAACATCGATCT+TGG - chr3_2:34957060-34957079 Msa0404190:five_prime_UTR 45.0%
!! TGTTGAGGTGGTCTTGTAAG+AGG + chr3_2:34957354-34957373 None:intergenic 45.0%
!! GATGGCGTTGACTGTATCAG+TGG + chr3_2:34954182-34954201 None:intergenic 50.0%
AAGACGGCTGAAGATGGAAG+GGG + chr3_2:34954279-34954298 None:intergenic 50.0%
GTAAGACGGCTGAAGATGGA+AGG + chr3_2:34954281-34954300 None:intergenic 50.0%
CATGGTAAGACGGCTGAAGA+TGG + chr3_2:34954285-34954304 None:intergenic 50.0%
AGATTGAGAGACGACGGTGA+TGG + chr3_2:34954312-34954331 None:intergenic 50.0%
GGCGAAAGATTGAGAGACGA+CGG + chr3_2:34954318-34954337 None:intergenic 50.0%
GTCGTCTCTCAATCTTTCGC+CGG - chr3_2:34954317-34954336 Msa0404190:three_prime_UTR 50.0%
CGAAAGTGATGGCTATGAGG+AGG + chr3_2:34954388-34954407 None:intergenic 50.0%
AGCCGAAAGTGATGGCTATG+AGG + chr3_2:34954391-34954410 None:intergenic 50.0%
AGCCATCACTTTCGGCTTCT+TGG - chr3_2:34954394-34954413 Msa0404190:intron 50.0%
AGTGCTCTTGAGACCCTTTG+TGG - chr3_2:34954511-34954530 Msa0404190:intron 50.0%
ACCCTTTGTGGCCAAGCTTA+CGG - chr3_2:34954523-34954542 Msa0404190:CDS 50.0%
CGCAAGGCAATACCACATGT+TGG - chr3_2:34954546-34954565 Msa0404190:CDS 50.0%
GGCGTGGTGAACACAAACAT+AGG + chr3_2:34954628-34954647 None:intergenic 50.0%
!! CTCTTGGTGGCTCTCAGTTT+TGG - chr3_2:34954887-34954906 Msa0404190:CDS 50.0%
! TTGGTGGGTGTCCACATTCA+TGG - chr3_2:34954931-34954950 Msa0404190:intron 50.0%
GGGTGTCCACATTCATGGAA+TGG - chr3_2:34954936-34954955 Msa0404190:intron 50.0%
TGGGAATCCATGATACCGCT+TGG - chr3_2:34955327-34955346 Msa0404190:intron 50.0%
GTCGCAGCCAAAAAACCAAG+CGG + chr3_2:34955345-34955364 None:intergenic 50.0%
!!! CTTGGTTTTTTGGCTGCGAC+CGG - chr3_2:34955345-34955364 Msa0404190:intron 50.0%
!!! TTGGTTTTTTGGCTGCGACC+GGG - chr3_2:34955346-34955365 Msa0404190:intron 50.0%
TTGCAGCTGTGATGCTGTTG+CGG - chr3_2:34955919-34955938 Msa0404190:intron 50.0%
CTCAATGTGACGATCCAACC+TGG - chr3_2:34956157-34956176 Msa0404190:intron 50.0%
AAAAGGGGCGAAATTCGCGA+CGG - chr3_2:34956394-34956413 Msa0404190:intron 50.0%
!! CGATTTTCAGGCGTTGCAGT+TGG - chr3_2:34956709-34956728 Msa0404190:CDS 50.0%
ATCTGGTCGTCAAGCAGTAG+TGG - chr3_2:34956732-34956751 Msa0404190:CDS 50.0%
ATACCTCTTGGGGTTCTCCT+AGG - chr3_2:34956790-34956809 Msa0404190:five_prime_UTR 50.0%
ACACTTACCACAATGCCGGA+AGG + chr3_2:34956826-34956845 None:intergenic 50.0%
GACTGGAATGATGAGCGGAA+CGG - chr3_2:34957039-34957058 Msa0404190:five_prime_UTR 50.0%
AGATGGAAGGGGCAGCAATG+TGG + chr3_2:34954268-34954287 None:intergenic 55.0%
!! GACGACGGTGATGGAGAACA+TGG + chr3_2:34954303-34954322 None:intergenic 55.0%
GGTGCAGGCAATGGAAATGG+CGG + chr3_2:34954366-34954385 None:intergenic 55.0%
! GAGGGTGCAGGCAATGGAAA+TGG + chr3_2:34954369-34954388 None:intergenic 55.0%
! TATGAGGAGGGTGCAGGCAA+TGG + chr3_2:34954375-34954394 None:intergenic 55.0%
GCCGTAAGCTTGGCCACAAA+GGG + chr3_2:34954527-34954546 None:intergenic 55.0%
ATTGCCTTGCGCCGTAAGCT+TGG + chr3_2:34954537-34954556 None:intergenic 55.0%
AGGACAACCTGTGGCTGTTG+CGG - chr3_2:34954663-34954682 Msa0404190:intron 55.0%
AACCTGTGGCTGTTGCGGTT+TGG - chr3_2:34954668-34954687 Msa0404190:intron 55.0%
AACCAAACCGCAACAGCCAC+AGG + chr3_2:34954673-34954692 None:intergenic 55.0%
TCGCTACTCGCACCCTGATA+TGG + chr3_2:34956353-34956372 None:intergenic 55.0%
GTGCGAGTAGCGAATGAGCT+TGG - chr3_2:34956360-34956379 Msa0404190:intron 55.0%
CTTGGCGCAGGCATGCAAAA+GGG - chr3_2:34956378-34956397 Msa0404190:intron 55.0%
TTGGCGCAGGCATGCAAAAG+GGG - chr3_2:34956379-34956398 Msa0404190:intron 55.0%
ATTCGCGACGGTGGTTTCAG+TGG - chr3_2:34956406-34956425 Msa0404190:intron 55.0%
! TCAGGCGTTGCAGTTGGATC+TGG - chr3_2:34956715-34956734 Msa0404190:CDS 55.0%
TGGAATGATGAGCGGAACGG+TGG - chr3_2:34957042-34957061 Msa0404190:five_prime_UTR 55.0%
GTCGAGATCGTTGAGGTGGC+CGG + chr3_2:34954339-34954358 None:intergenic 60.0%
CGAGGTCGAGATCGTTGAGG+TGG + chr3_2:34954343-34954362 None:intergenic 60.0%
AGGCAATGGAAATGGCGGCG+AGG + chr3_2:34954361-34954380 None:intergenic 60.0%
! GATGGCTATGAGGAGGGTGC+AGG + chr3_2:34954381-34954400 None:intergenic 60.0%
CGCCGTAAGCTTGGCCACAA+AGG + chr3_2:34954528-34954547 None:intergenic 60.0%
GCTGCGACCGGGTATGTTTC+TGG - chr3_2:34955357-34955376 Msa0404190:intron 60.0%
GAGGGGAAGTCTCACCAGGT+TGG + chr3_2:34956174-34956193 None:intergenic 60.0%
ACCTGGTGAGACTTCCCCTC+AGG - chr3_2:34956174-34956193 Msa0404190:intron 60.0%
CCTGGTGAGACTTCCCCTCA+GGG - chr3_2:34956175-34956194 Msa0404190:intron 60.0%
CTGGAGTTGTTGGCCCTGAG+GGG + chr3_2:34956191-34956210 None:intergenic 60.0%
GCTGGAGTTGTTGGCCCTGA+GGG + chr3_2:34956192-34956211 None:intergenic 60.0%
AGCTGGAGTTGTTGGCCCTG+AGG + chr3_2:34956193-34956212 None:intergenic 60.0%
GTAGCGAATGAGCTTGGCGC+AGG - chr3_2:34956366-34956385 Msa0404190:intron 60.0%
GCTTGGCGCAGGCATGCAAA+AGG - chr3_2:34956377-34956396 Msa0404190:intron 60.0%
CAGCCTAGGAGAACCCCAAG+AGG + chr3_2:34956796-34956815 None:intergenic 60.0%
CTCTTGGGGTTCTCCTAGGC+TGG - chr3_2:34956794-34956813 Msa0404190:five_prime_UTR 60.0%
! CTAGGCTGGCTGCTACCTTC+CGG - chr3_2:34956808-34956827 Msa0404190:five_prime_UTR 60.0%
GCTGCTACCTTCCGGCATTG+TGG - chr3_2:34956816-34956835 Msa0404190:five_prime_UTR 60.0%
CGGCGAGGTCGAGATCGTTG+AGG + chr3_2:34954346-34954365 None:intergenic 65.0%
GTGGCCAAGCTTACGGCGCA+AGG - chr3_2:34954530-34954549 Msa0404190:CDS 65.0%
CCCTGAGGGGAAGTCTCACC+AGG + chr3_2:34956178-34956197 None:intergenic 65.0%
AGGGGCGAAATTCGCGACGG+TGG - chr3_2:34956397-34956416 Msa0404190:intron 65.0%
CGGAAGGTAGCAGCCAGCCT+AGG + chr3_2:34956810-34956829 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 34954142 34957491 34954142 ID=Msa0404190;Name=Msa0404190
chr3_2 mRNA 34954142 34957491 34954142 ID=Msa0404190-mRNA-1;Parent=Msa0404190;Name=Msa0404190-mRNA-1;_AED=0.34;_eAED=0.34;_QI=621|0.85|0.87|1|0.85|0.62|8|219|264
chr3_2 exon 34954142 34954411 34954142 ID=Msa0404190-mRNA-1:exon:7161;Parent=Msa0404190-mRNA-1
chr3_2 exon 34954516 34954602 34954516 ID=Msa0404190-mRNA-1:exon:7160;Parent=Msa0404190-mRNA-1
chr3_2 exon 34954796 34954914 34954796 ID=Msa0404190-mRNA-1:exon:7159;Parent=Msa0404190-mRNA-1
chr3_2 exon 34955037 34955245 34955037 ID=Msa0404190-mRNA-1:exon:7158;Parent=Msa0404190-mRNA-1
chr3_2 exon 34956266 34956322 34956266 ID=Msa0404190-mRNA-1:exon:7157;Parent=Msa0404190-mRNA-1
chr3_2 exon 34956427 34956513 34956427 ID=Msa0404190-mRNA-1:exon:7156;Parent=Msa0404190-mRNA-1
chr3_2 exon 34956603 34957134 34956603 ID=Msa0404190-mRNA-1:exon:7155;Parent=Msa0404190-mRNA-1
chr3_2 exon 34957218 34957491 34957218 ID=Msa0404190-mRNA-1:exon:7154;Parent=Msa0404190-mRNA-1
chr3_2 five_prime_UTR 34957218 34957491 34957218 ID=Msa0404190-mRNA-1:five_prime_utr;Parent=Msa0404190-mRNA-1
chr3_2 five_prime_UTR 34956788 34957134 34956788 ID=Msa0404190-mRNA-1:five_prime_utr;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34956603 34956787 34956603 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34956427 34956513 34956427 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34956266 34956322 34956266 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34955037 34955245 34955037 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34954796 34954914 34954796 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34954516 34954602 34954516 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 CDS 34954361 34954411 34954361 ID=Msa0404190-mRNA-1:cds;Parent=Msa0404190-mRNA-1
chr3_2 three_prime_UTR 34954142 34954360 34954142 ID=Msa0404190-mRNA-1:three_prime_utr;Parent=Msa0404190-mRNA-1
Gene Sequence

>Msa0404190

ATGAGAGTTGGGATTGTTGGGATTGCTCTTACCATTGATTTCTCTTGGTGGCTCTCAGTTTTGGGAATGTTTGTGTATACACTCTTTGGTGGGTGTCCACATTCATGGAATGGTTTTTCAGCTGAAGCTTTTGTTGGTTTGTGGGATTTCTTTAAACTATCTTTGGCTTCTGGTGTCATGCTTGCGTTAGAGAACTTCTATTATAGAATGTTGCTTATAATGTCTCGTTATATGTACAACTCTGATGTTGCAATCGATGCTCTTTCGGTTTGTGTAACTATTTATGGTTGGGAATCCATGATACCGCTTGGTTTTTTGGCTGCGACCGGGCATGCAAAAGGGGCGAAATTCGCGACGGTGGTTTCAGTGGTAAATACTGTGATCGTTGGATTCATATTTTGGTTGATAATTGTGGTTTTCAATGAGAAGCTTGCTTTGATCTTTACATCAAGCTTATCTGTAATCCAAATGGTCAATGAACTATCAATCTTGTTGGCTTTTACTATTCTTCTTAATTGCATTCAACCTGTTCTTTCAGGCGTTGCAGTTGGATCTGGTCGTCAAGCAGTAGTGGCTTATATAAACATTGGTAGTTACTACTTGGTTGGAATACCTCTTGGGGTTCTCCTAGGCTGGCTGCTACCTTCCGGCATTGTGGGTATGTGGACTGGAATGATGAGCGGAACGGTGGTTCAAACATCGATCTTGGCCATTATTATGTTGAGATACAATTGGGAAAGAGAGGTACGGAATGTCCAAATTCATGTTAAGGACGACACAACGTCCAACCAATAA
Protein sequence

>Msa0404190

MRVGIVGIALTIDFSWWLSVLGMFVYTLFGGCPHSWNGFSAEAFVGLWDFFKLSLASGVMLALENFYYRMLLIMSRYMYNSDVAIDALSVCVTIYGWESMIPLGFLAATGHAKGAKFATVVSVVNTVIVGFIFWLIIVVFNEKLALIFTSSLSVIQMVNELSILLAFTILLNCIQPVLSGVAVGSGRQAVVAYINIGSYYLVGIPLGVLLGWLLPSGIVGMWTGMMSGTVVQTSILAIIMLRYNWEREVRNVQIHVKDDTTSNQ*