Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0273770 | XP_013462645.1 | 95.420 | 131 | 2 | 1 | 1 | 131 | 1 | 127 | 4.57e-84 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0273770 | A0A072VET2 | 95.420 | 131 | 2 | 1 | 1 | 131 | 1 | 127 | 2.18e-84 | 251 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016510 | Msa0273770 | 0.810998 | 8.720764e-51 | -8.615850e-47 |
| Msa0032080 | Msa0273770 | 0.808130 | 3.603331e-50 | -8.615850e-47 |
| Msa0055640 | Msa0273770 | 0.806812 | 6.859357e-50 | -8.615850e-47 |
| Msa0090550 | Msa0273770 | 0.803422 | 3.512133e-49 | -8.615850e-47 |
| Msa0090970 | Msa0273770 | 0.810728 | 9.977787e-51 | -8.615850e-47 |
| Msa0150230 | Msa0273770 | 0.810553 | 1.088832e-50 | -8.615850e-47 |
| Msa0172080 | Msa0273770 | 0.803962 | 2.713481e-49 | -8.615850e-47 |
| Msa0183450 | Msa0273770 | 0.813326 | 2.709204e-51 | -8.615850e-47 |
| Msa0186950 | Msa0273770 | 0.805910 | 1.062416e-49 | -8.615850e-47 |
| Msa0187490 | Msa0273770 | 0.807993 | 3.852191e-50 | -8.615850e-47 |
| Msa0196670 | Msa0273770 | 0.807341 | 5.298720e-50 | -8.615850e-47 |
| Msa0273770 | Msa0284450 | 0.803692 | 3.087397e-49 | -8.615850e-47 |
| Msa0273770 | Msa0316390 | 0.815671 | 8.203686e-52 | -8.615850e-47 |
| Msa0273770 | Msa0326750 | 0.804468 | 2.129612e-49 | -8.615850e-47 |
| Msa0273770 | Msa0347750 | 0.807551 | 4.781970e-50 | -8.615850e-47 |
| Msa0273770 | Msa0403490 | 0.803677 | 3.110021e-49 | -8.615850e-47 |
| Msa0273770 | Msa0439980 | 0.813579 | 2.383137e-51 | -8.615850e-47 |
| Msa0273770 | Msa0518170 | 0.812475 | 4.161008e-51 | -8.615850e-47 |
| Msa0273770 | Msa0527740 | 0.816867 | 4.432638e-52 | -8.615850e-47 |
| Msa0273770 | Msa0662540 | 0.806190 | 9.275951e-50 | -8.615850e-47 |
| Msa0273770 | Msa0705990 | 0.807447 | 5.032353e-50 | -8.615850e-47 |
| Msa0273770 | Msa0750850 | 0.815549 | 8.734379e-52 | -8.615850e-47 |
| Msa0273770 | Msa0970480 | 0.809756 | 1.616842e-50 | -8.615850e-47 |
| Msa0273770 | Msa0994370 | 0.842069 | 3.238914e-58 | -8.615850e-47 |
| Msa0273770 | Msa1026640 | 0.825502 | 4.527277e-54 | -8.615850e-47 |
| Msa0273770 | Msa1042660 | 0.827673 | 1.374167e-54 | -8.615850e-47 |
| Msa0273770 | Msa1063420 | 0.806667 | 7.359037e-50 | -8.615850e-47 |
| Msa0273770 | Msa1071320 | 0.802924 | 4.452320e-49 | -8.615850e-47 |
| Msa0273770 | Msa1081790 | -0.808243 | 3.408490e-50 | -8.615850e-47 |
| Msa0273770 | Msa1107260 | 0.815860 | 7.446310e-52 | -8.615850e-47 |
| Msa0273770 | Msa1150900 | 0.805345 | 1.395924e-49 | -8.615850e-47 |
| Msa0273770 | Msa1207310 | 0.810667 | 1.028478e-50 | -8.615850e-47 |
| Msa0273770 | Msa1270660 | 0.835669 | 1.466682e-56 | -8.615850e-47 |
| Msa0273770 | Msa1270730 | 0.841619 | 4.258005e-58 | -8.615850e-47 |
| Msa0273770 | Msa1280170 | 0.805989 | 1.022719e-49 | -8.615850e-47 |
| Msa0273770 | Msa1291450 | 0.804933 | 1.702444e-49 | -8.615850e-47 |
| Msa0273770 | Msa1315750 | 0.803738 | 3.020494e-49 | -8.615850e-47 |
| Msa0273770 | Msa1316360 | 0.809388 | 1.939960e-50 | -8.615850e-47 |
| Msa0273770 | Msa1316440 | 0.822300 | 2.550816e-53 | -8.615850e-47 |
| Msa0273770 | Msa1335230 | 0.842559 | 2.401710e-58 | -8.615850e-47 |
| Msa0273770 | Msa1423820 | 0.832042 | 1.184745e-55 | -8.615850e-47 |
| Msa0273770 | Msa1428780 | 0.829203 | 5.868958e-55 | -8.615850e-47 |
| Msa0273770 | Msa1437730 | 0.801683 | 8.019857e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0273770 | MtrunA17_Chr2g0285141 | 95.420 | 131 | 2 | 1 | 1 | 131 | 1 | 127 | 4.20e-88 | 251 |
| Msa0273770 | MtrunA17_Chr2g0288501 | 50.602 | 83 | 33 | 3 | 56 | 130 | 74 | 156 | 1.58e-16 | 71.2 |
| Msa0273770 | MtrunA17_Chr3g0099101 | 61.224 | 49 | 17 | 1 | 84 | 130 | 150 | 198 | 6.56e-13 | 62.8 |
| Msa0273770 | MtrunA17_Chr2g0326661 | 45.349 | 86 | 33 | 4 | 54 | 128 | 72 | 154 | 5.97e-12 | 59.3 |
| Msa0273770 | MtrunA17_Chr7g0266691 | 56.250 | 48 | 19 | 1 | 85 | 130 | 170 | 217 | 6.26e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0273770 | AT1G29640.1 | 39.310 | 145 | 69 | 5 | 1 | 131 | 1 | 140 | 1.70e-24 | 91.7 |
| Msa0273770 | AT2G34340.1 | 41.727 | 139 | 70 | 4 | 1 | 131 | 1 | 136 | 2.38e-23 | 88.6 |
| Msa0273770 | AT5G45630.1 | 35.878 | 131 | 67 | 3 | 1 | 131 | 1 | 114 | 2.34e-19 | 77.8 |
| Msa0273770 | AT4G18980.1 | 41.481 | 135 | 46 | 7 | 1 | 131 | 1 | 106 | 8.96e-19 | 75.9 |
| Msa0273770 | AT4G21970.1 | 46.667 | 90 | 29 | 3 | 56 | 130 | 60 | 145 | 1.66e-18 | 76.6 |
| Msa0273770 | AT4G04630.1 | 64.000 | 50 | 16 | 1 | 83 | 130 | 119 | 168 | 3.25e-15 | 68.6 |
| Msa0273770 | AT3G15040.1 | 56.863 | 51 | 20 | 1 | 82 | 130 | 193 | 243 | 6.83e-12 | 60.8 |
| Msa0273770 | AT2G28400.1 | 31.690 | 142 | 76 | 5 | 2 | 128 | 25 | 160 | 4.48e-11 | 57.4 |
Find 48 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTAGGAATTCGATTCTT+AGG | 0.266852 | 2_3:-5354060 | Msa0273770:CDS |
| CAGCTTCAGATTGATGAATC+TGG | 0.295768 | 2_3:+5354368 | None:intergenic |
| GGTGCCACCGCACCTGATTA+TGG | 0.319375 | 2_3:-5354161 | Msa0273770:CDS |
| CTAGCCTCCGCGCCATAATC+AGG | 0.322627 | 2_3:+5354149 | None:intergenic |
| GAAGGGATTTGAGCAGGGTT+AGG | 0.370718 | 2_3:-5354075 | Msa0273770:CDS |
| TGAAGGGAAGGGATTTGAGC+AGG | 0.444324 | 2_3:-5354081 | Msa0273770:CDS |
| TGGGCAAGGGAGGACTTTGA+AGG | 0.455352 | 2_3:-5354098 | Msa0273770:CDS |
| ATTATGGCGCGGAGGCTAGC+TGG | 0.455420 | 2_3:-5354145 | Msa0273770:CDS |
| ATATTTCACCGGAGGGGTAA+TGG | 0.465755 | 2_3:-5354214 | Msa0273770:CDS |
| AATTCGATTCTTAGGATGAC+TGG | 0.471954 | 2_3:-5354052 | Msa0273770:CDS |
| TTTCCATGTGTTCTGGGCAA+GGG | 0.507966 | 2_3:-5354111 | Msa0273770:CDS |
| GGTGCTGCTGAAGCTGAAGA+AGG | 0.521781 | 2_3:-5354335 | Msa0273770:CDS |
| CTATGAGGAGGACGAGGATG+AGG | 0.526881 | 2_3:-5354191 | Msa0273770:CDS |
| CCGGAGGGGTAATGGCTATG+AGG | 0.530842 | 2_3:-5354206 | Msa0273770:CDS |
| GGACGAGGATGAGGAGGAGA+TGG | 0.533776 | 2_3:-5354182 | Msa0273770:CDS |
| CCTCATAGCCATTACCCCTC+CGG | 0.534896 | 2_3:+5354206 | None:intergenic |
| CCTCCCTTGCCCAGAACACA+TGG | 0.536919 | 2_3:+5354108 | None:intergenic |
| CAACAACAACAAGAAGAAGA+TGG | 0.538464 | 2_3:-5354272 | Msa0273770:CDS |
| CCCTCGGAGGGGATATTCAT+CGG | 0.546466 | 2_3:+5354238 | None:intergenic |
| CCATGTGTTCTGGGCAAGGG+AGG | 0.550827 | 2_3:-5354108 | Msa0273770:CDS |
| AGGGAGGACTTTGAAGGGAA+GGG | 0.555164 | 2_3:-5354092 | Msa0273770:CDS |
| CCTCCGAGGGGATATTTCAC+CGG | 0.557899 | 2_3:-5354225 | Msa0273770:CDS |
| GGGCAAGGGAGGACTTTGAA+GGG | 0.559015 | 2_3:-5354097 | Msa0273770:CDS |
| CGGTGAAATATCCCCTCGGA+GGG | 0.562093 | 2_3:+5354226 | None:intergenic |
| TGAGGAGTTTCTTGAATCCG+AGG | 0.568990 | 2_3:-5354404 | Msa0273770:CDS |
| ATCTGAAGCTGAAGACAAGA+AGG | 0.569372 | 2_3:-5354356 | Msa0273770:CDS |
| AAGGGAGGACTTTGAAGGGA+AGG | 0.573855 | 2_3:-5354093 | Msa0273770:CDS |
| GGAGGGGATATTCATCGGCA+TGG | 0.583795 | 2_3:+5354243 | None:intergenic |
| CCGATGAATATCCCCTCCGA+GGG | 0.587173 | 2_3:-5354238 | Msa0273770:CDS |
| GAAGGGAAGGGATTTGAGCA+GGG | 0.588262 | 2_3:-5354080 | Msa0273770:CDS |
| TAATGGCTATGAGGAGGACG+AGG | 0.588381 | 2_3:-5354197 | Msa0273770:CDS |
| CGCGCCATAATCAGGTGCGG+TGG | 0.596723 | 2_3:+5354157 | None:intergenic |
| TAACTTCACCAACATGTCTG+AGG | 0.596960 | 2_3:-5354422 | None:intergenic |
| TGAGGAGGACGAGGATGAGG+AGG | 0.598626 | 2_3:-5354188 | Msa0273770:CDS |
| CCGAGGGGATATTTCACCGG+AGG | 0.610079 | 2_3:-5354222 | Msa0273770:CDS |
| GCTGCTGAAGCTGAAGAAGG+AGG | 0.612278 | 2_3:-5354332 | Msa0273770:CDS |
| CGAGGGGATATTTCACCGGA+GGG | 0.625487 | 2_3:-5354221 | Msa0273770:CDS |
| CTCCGCGCCATAATCAGGTG+CGG | 0.652646 | 2_3:+5354154 | None:intergenic |
| CACCGCACCTGATTATGGCG+CGG | 0.652859 | 2_3:-5354156 | Msa0273770:CDS |
| CCTCCGGTGAAATATCCCCT+CGG | 0.659237 | 2_3:+5354222 | None:intergenic |
| GCCGATGAATATCCCCTCCG+AGG | 0.660447 | 2_3:-5354239 | Msa0273770:CDS |
| CGCACCTGATTATGGCGCGG+AGG | 0.660768 | 2_3:-5354153 | Msa0273770:CDS |
| GAGGGGTAATGGCTATGAGG+AGG | 0.676604 | 2_3:-5354203 | Msa0273770:CDS |
| CCGGTGAAATATCCCCTCGG+AGG | 0.677235 | 2_3:+5354225 | None:intergenic |
| ACAACAAGAAGAAGATGGTG+AGG | 0.679378 | 2_3:-5354267 | Msa0273770:CDS |
| GGTGAAATATCCCCTCGGAG+GGG | 0.694267 | 2_3:+5354227 | None:intergenic |
| CGATGAATATCCCCTCCGAG+GGG | 0.743161 | 2_3:-5354237 | Msa0273770:CDS |
| GAGGGGATATTTCACCGGAG+GGG | 0.792038 | 2_3:-5354220 | Msa0273770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGGTTGAAAAAAAGAACCT+CGG | + | chr2_3:5354061-5354080 | None:intergenic | 35.0% | |
| CAACAACAACAAGAAGAAGA+TGG | - | chr2_3:5354173-5354192 | Msa0273770:CDS | 35.0% | |
| ! | ATGACATTTTCCATGTGTTC+TGG | - | chr2_3:5354327-5354346 | Msa0273770:CDS | 35.0% |
| ! | TGACATTTTCCATGTGTTCT+GGG | - | chr2_3:5354328-5354347 | Msa0273770:CDS | 35.0% |
| AATTCGATTCTTAGGATGAC+TGG | - | chr2_3:5354393-5354412 | Msa0273770:CDS | 35.0% | |
| !!! | TAGGATGACTGGTTTTATTG+AGG | - | chr2_3:5354404-5354423 | Msa0273770:CDS | 35.0% |
| CAGCTTCAGATTGATGAATC+TGG | + | chr2_3:5354080-5354099 | None:intergenic | 40.0% | |
| ! | ATCTGAAGCTGAAGACAAGA+AGG | - | chr2_3:5354089-5354108 | Msa0273770:CDS | 40.0% |
| ACAACAAGAAGAAGATGGTG+AGG | - | chr2_3:5354178-5354197 | Msa0273770:CDS | 40.0% | |
| GGGTTAGGAATTCGATTCTT+AGG | - | chr2_3:5354385-5354404 | Msa0273770:CDS | 40.0% | |
| TGAGGAGTTTCTTGAATCCG+AGG | - | chr2_3:5354041-5354060 | Msa0273770:CDS | 45.0% | |
| ATATTTCACCGGAGGGGTAA+TGG | - | chr2_3:5354231-5354250 | Msa0273770:CDS | 45.0% | |
| !! | TTTTCCATGTGTTCTGGGCA+AGG | - | chr2_3:5354333-5354352 | Msa0273770:CDS | 45.0% |
| ! | TTTCCATGTGTTCTGGGCAA+GGG | - | chr2_3:5354334-5354353 | Msa0273770:CDS | 45.0% |
| TAATGGCTATGAGGAGGACG+AGG | - | chr2_3:5354248-5354267 | Msa0273770:CDS | 50.0% | |
| ! | AAGGGAGGACTTTGAAGGGA+AGG | - | chr2_3:5354352-5354371 | Msa0273770:CDS | 50.0% |
| ! | AGGGAGGACTTTGAAGGGAA+GGG | - | chr2_3:5354353-5354372 | Msa0273770:CDS | 50.0% |
| TGAAGGGAAGGGATTTGAGC+AGG | - | chr2_3:5354364-5354383 | Msa0273770:CDS | 50.0% | |
| GAAGGGAAGGGATTTGAGCA+GGG | - | chr2_3:5354365-5354384 | Msa0273770:CDS | 50.0% | |
| GAAGGGATTTGAGCAGGGTT+AGG | - | chr2_3:5354370-5354389 | Msa0273770:CDS | 50.0% | |
| !! | GGTGCTGCTGAAGCTGAAGA+AGG | - | chr2_3:5354110-5354129 | Msa0273770:CDS | 55.0% |
| GCTGCTGAAGCTGAAGAAGG+AGG | - | chr2_3:5354113-5354132 | Msa0273770:CDS | 55.0% | |
| GGAGGGGATATTCATCGGCA+TGG | + | chr2_3:5354205-5354224 | None:intergenic | 55.0% | |
| CCCTCGGAGGGGATATTCAT+CGG | + | chr2_3:5354210-5354229 | None:intergenic | 55.0% | |
| CCGATGAATATCCCCTCCGA+GGG | - | chr2_3:5354207-5354226 | Msa0273770:CDS | 55.0% | |
| CGATGAATATCCCCTCCGAG+GGG | - | chr2_3:5354208-5354227 | Msa0273770:CDS | 55.0% | |
| GGTGAAATATCCCCTCGGAG+GGG | + | chr2_3:5354221-5354240 | None:intergenic | 55.0% | |
| CGGTGAAATATCCCCTCGGA+GGG | + | chr2_3:5354222-5354241 | None:intergenic | 55.0% | |
| CCTCCGAGGGGATATTTCAC+CGG | - | chr2_3:5354220-5354239 | Msa0273770:CDS | 55.0% | |
| CCTCCGGTGAAATATCCCCT+CGG | + | chr2_3:5354226-5354245 | None:intergenic | 55.0% | |
| CGAGGGGATATTTCACCGGA+GGG | - | chr2_3:5354224-5354243 | Msa0273770:CDS | 55.0% | |
| GAGGGGATATTTCACCGGAG+GGG | - | chr2_3:5354225-5354244 | Msa0273770:CDS | 55.0% | |
| CCTCATAGCCATTACCCCTC+CGG | + | chr2_3:5354242-5354261 | None:intergenic | 55.0% | |
| ! | GAGGGGTAATGGCTATGAGG+AGG | - | chr2_3:5354242-5354261 | Msa0273770:CDS | 55.0% |
| CTATGAGGAGGACGAGGATG+AGG | - | chr2_3:5354254-5354273 | Msa0273770:CDS | 55.0% | |
| TGGGCAAGGGAGGACTTTGA+AGG | - | chr2_3:5354347-5354366 | Msa0273770:CDS | 55.0% | |
| GGGCAAGGGAGGACTTTGAA+GGG | - | chr2_3:5354348-5354367 | Msa0273770:CDS | 55.0% | |
| GCCGATGAATATCCCCTCCG+AGG | - | chr2_3:5354206-5354225 | Msa0273770:CDS | 60.0% | |
| CCGGTGAAATATCCCCTCGG+AGG | + | chr2_3:5354223-5354242 | None:intergenic | 60.0% | |
| CCGAGGGGATATTTCACCGG+AGG | - | chr2_3:5354223-5354242 | Msa0273770:CDS | 60.0% | |
| ! | CCGGAGGGGTAATGGCTATG+AGG | - | chr2_3:5354239-5354258 | Msa0273770:CDS | 60.0% |
| TGAGGAGGACGAGGATGAGG+AGG | - | chr2_3:5354257-5354276 | Msa0273770:CDS | 60.0% | |
| GGACGAGGATGAGGAGGAGA+TGG | - | chr2_3:5354263-5354282 | Msa0273770:CDS | 60.0% | |
| ! | GGTGCCACCGCACCTGATTA+TGG | - | chr2_3:5354284-5354303 | Msa0273770:CDS | 60.0% |
| CACCGCACCTGATTATGGCG+CGG | - | chr2_3:5354289-5354308 | Msa0273770:CDS | 60.0% | |
| CTCCGCGCCATAATCAGGTG+CGG | + | chr2_3:5354294-5354313 | None:intergenic | 60.0% | |
| CTAGCCTCCGCGCCATAATC+AGG | + | chr2_3:5354299-5354318 | None:intergenic | 60.0% | |
| ATTATGGCGCGGAGGCTAGC+TGG | - | chr2_3:5354300-5354319 | Msa0273770:CDS | 60.0% | |
| CCTCCCTTGCCCAGAACACA+TGG | + | chr2_3:5354340-5354359 | None:intergenic | 60.0% | |
| ! | CCATGTGTTCTGGGCAAGGG+AGG | - | chr2_3:5354337-5354356 | Msa0273770:CDS | 60.0% |
| CGCGCCATAATCAGGTGCGG+TGG | + | chr2_3:5354291-5354310 | None:intergenic | 65.0% | |
| CGCACCTGATTATGGCGCGG+AGG | - | chr2_3:5354292-5354311 | Msa0273770:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 5354036 | 5354431 | 5354036 | ID=Msa0273770;Name=Msa0273770 |
| chr2_3 | mRNA | 5354036 | 5354431 | 5354036 | ID=Msa0273770-mRNA-1;Parent=Msa0273770;Name=Msa0273770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|131 |
| chr2_3 | exon | 5354036 | 5354431 | 5354036 | ID=Msa0273770-mRNA-1:exon:2563;Parent=Msa0273770-mRNA-1 |
| chr2_3 | CDS | 5354036 | 5354431 | 5354036 | ID=Msa0273770-mRNA-1:cds;Parent=Msa0273770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
