AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662540 MtrunA17_Chr4g0070871 90.222 225 19 1 2 223 208 432 4.98e-148 422
Msa0662540 MtrunA17_Chr2g0299981 65.587 247 59 3 2 223 222 467 2.64e-107 319
Msa0662540 MtrunA17_Chr1g0150781 61.864 236 77 3 2 224 241 476 1.06e-93 285
Msa0662540 MtrunA17_Chr3g0135961 59.336 241 78 4 2 223 232 471 1.62e-93 284
Msa0662540 MtrunA17_Chr3g0112291 48.918 231 94 3 1 223 93 307 1.44e-66 210
Msa0662540 MtrunA17_Chr8g0389921 48.085 235 100 5 2 224 150 374 2.98e-64 206
Msa0662540 MtrunA17_Chr5g0435721 50.000 230 89 3 2 223 194 405 1.04e-62 202
Msa0662540 MtrunA17_Chr5g0396731 46.753 231 94 4 2 223 74 284 2.39e-59 190
Msa0662540 MtrunA17_Chr1g0158781 51.121 223 88 5 2 223 114 316 5.48e-54 177
Msa0662540 MtrunA17_Chr3g0126511 38.938 226 106 5 2 223 96 293 6.68e-39 137
Msa0662540 MtrunA17_Chr3g0136711 44.809 183 85 4 45 223 63 233 3.71e-38 134
Msa0662540 MtrunA17_Chr3g0105371 37.168 226 111 6 2 223 87 285 7.29e-38 134
Msa0662540 MtrunA17_Chr1g0150451 40.265 226 103 6 2 223 79 276 1.41e-37 133
Msa0662540 MtrunA17_Chr3g0105351 36.283 226 113 6 2 223 63 261 2.04e-37 132
Msa0662540 MtrunA17_Chr1g0212961 39.474 228 98 8 2 223 97 290 2.49e-36 130
Msa0662540 MtrunA17_Chr5g0430481 40.782 179 93 4 47 223 154 321 6.43e-35 127
Msa0662540 MtrunA17_Chr4g0050381 38.830 188 97 5 41 223 55 229 1.76e-27 105
Msa0662540 MtrunA17_Chr3g0136811 33.579 271 109 11 2 223 25 273 1.03e-25 102
Msa0662540 MtrunA17_Chr4g0001761 31.047 277 110 10 1 223 24 273 7.08e-25 100
Msa0662540 MtrunA17_Chr1g0171571 35.652 230 118 10 2 223 389 596 9.18e-24 99.0
Msa0662540 MtrunA17_Chr1g0150071 32.222 270 114 10 2 223 25 273 1.14e-23 97.1
Msa0662540 MtrunA17_Chr2g0279151 39.362 188 90 6 44 223 62 233 1.66e-23 95.5
Msa0662540 MtrunA17_Chr5g0426141 37.297 185 98 6 44 223 63 234 6.84e-23 94.0
Msa0662540 MtrunA17_Chr8g0360351 36.082 194 102 6 35 223 58 234 7.13e-23 93.6
Msa0662540 MtrunA17_Chr4g0051661 36.667 180 99 4 47 223 183 350 9.68e-23 95.1
Msa0662540 MtrunA17_Chr3g0115851 37.297 185 98 6 44 223 63 234 1.06e-22 93.2
Msa0662540 MtrunA17_Chr1g0194191 31.674 221 127 8 19 223 86 298 1.87e-22 94.4
Msa0662540 MtrunA17_Chr5g0407161 32.632 190 105 6 42 223 120 294 3.36e-22 93.2
Msa0662540 MtrunA17_Chr2g0317021 36.000 200 107 6 32 223 99 285 2.36e-21 90.5
Msa0662540 MtrunA17_Chr4g0065141 32.821 195 98 6 45 223 122 299 4.09e-21 90.5
Msa0662540 MtrunA17_Chr2g0293811 32.500 200 118 7 35 223 87 280 1.20e-19 86.3
Msa0662540 MtrunA17_Chr4g0066371 31.390 223 95 11 43 223 82 288 9.91e-19 84.0
Msa0662540 MtrunA17_Chr3g0144111 34.021 194 82 8 39 223 20 176 2.48e-18 81.3
Msa0662540 MtrunA17_Chr3g0138281 32.161 199 109 7 36 223 111 294 2.63e-18 82.4
Msa0662540 MtrunA17_Chr7g0225501 33.846 195 113 10 43 223 80 272 9.86e-18 81.3
Msa0662540 MtrunA17_Chr1g0185141 32.258 217 90 8 56 223 171 379 1.79e-17 80.9
Msa0662540 MtrunA17_Chr7g0263281 29.412 221 107 6 43 223 67 278 1.15e-16 77.8
Msa0662540 MtrunA17_Chr1g0207571 29.545 220 110 7 43 223 57 270 3.42e-16 76.6
Msa0662540 MtrunA17_Chr6g0483411 31.863 204 94 9 48 223 80 266 3.59e-16 75.9
Msa0662540 MtrunA17_Chr2g0286951 30.942 223 96 9 43 223 83 289 8.35e-16 75.5
Msa0662540 MtrunA17_Chr1g0211251 28.063 253 139 8 6 223 44 288 3.51e-15 73.6
Msa0662540 MtrunA17_Chr1g0181901 28.636 220 124 4 36 223 65 283 5.02e-15 73.2
Msa0662540 MtrunA17_Chr1g0209451 31.818 198 116 9 37 223 61 250 6.66e-15 72.8
Msa0662540 MtrunA17_Chr1g0192421 32.536 209 108 9 47 223 89 296 2.44e-14 70.9
Msa0662540 MtrunA17_Chr7g0250061 29.907 214 115 6 43 223 72 283 3.63e-14 70.9
Msa0662540 MtrunA17_Chr7g0243571 31.963 219 107 9 47 223 92 310 5.01e-14 70.5
Msa0662540 MtrunA17_Chr4g0004971 31.132 212 111 6 47 223 78 289 8.75e-14 69.3
Msa0662540 MtrunA17_Chr7g0272061 30.734 218 109 9 47 223 91 307 9.79e-14 69.3
Msa0662540 MtrunA17_Chr4g0067711 31.250 208 112 9 47 223 91 298 1.63e-13 68.6
Msa0662540 MtrunA17_Chr7g0271971 30.928 194 116 7 43 223 62 250 2.65e-13 68.2
Msa0662540 MtrunA17_Chr7g0256771 27.273 231 133 6 26 223 49 277 3.25e-13 67.4
Msa0662540 MtrunA17_Chr3g0133801 31.551 187 112 8 48 223 67 248 9.00e-13 66.6
Msa0662540 MtrunA17_Chr1g0151781 30.435 207 114 7 47 223 68 274 1.82e-12 65.5
Msa0662540 MtrunA17_Chr7g0256751 28.708 209 116 7 47 223 69 276 2.95e-12 65.1
Msa0662540 MtrunA17_Chr3g0089711 28.846 208 117 5 47 223 68 275 7.03e-12 63.9
Msa0662540 MtrunA17_Chr3g0089721 27.511 229 135 5 26 223 47 275 7.18e-12 63.9
Msa0662540 MtrunA17_Chr8g0371321 27.189 217 117 5 43 223 75 286 1.12e-11 63.5
Msa0662540 MtrunA17_Chr1g0192471 29.947 187 115 6 48 223 69 250 2.56e-11 62.4
Msa0662540 MtrunA17_Chr1g0153671 30.481 187 114 8 48 223 66 247 2.91e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662540 AT4G26080.1 60.811 222 84 1 2 223 130 348 7.02e-91 274
Msa0662540 AT5G57050.2 60.000 225 87 2 2 223 74 298 2.57e-88 266
Msa0662540 AT5G57050.1 60.000 225 87 2 2 223 114 338 4.16e-88 266
Msa0662540 AT1G72770.4 56.504 246 82 5 2 224 191 434 3.79e-86 264
Msa0662540 AT1G72770.1 56.504 246 82 5 2 224 191 434 3.79e-86 264
Msa0662540 AT1G72770.3 56.504 246 82 5 2 224 191 434 3.79e-86 264
Msa0662540 AT1G17550.1 55.691 246 83 5 2 223 190 433 7.89e-86 263
Msa0662540 AT1G17550.2 55.691 246 83 5 2 223 190 433 7.89e-86 263
Msa0662540 AT5G57050.3 57.868 197 79 3 2 195 114 309 1.63e-70 218
Msa0662540 AT1G72770.2 54.460 213 72 5 2 191 191 401 3.57e-67 212
Msa0662540 AT1G72770.5 54.460 213 72 5 2 191 191 401 3.57e-67 212
Msa0662540 AT2G29380.1 48.498 233 94 6 2 223 79 296 5.02e-65 206
Msa0662540 AT3G11410.1 45.643 241 98 5 1 224 105 329 1.02e-63 203
Msa0662540 AT5G59220.1 49.561 228 97 5 2 223 113 328 4.51e-62 199
Msa0662540 AT1G07430.1 46.320 231 103 4 2 223 122 340 1.13e-60 196
Msa0662540 AT5G51760.2 47.718 241 97 5 2 224 80 309 6.66e-56 182
Msa0662540 AT5G51760.1 47.917 240 96 5 2 223 110 338 1.49e-55 182
Msa0662540 AT5G59220.2 48.469 196 83 5 2 191 113 296 3.08e-48 160
Msa0662540 AT5G10740.1 41.810 232 91 9 2 223 35 232 4.10e-38 135
Msa0662540 AT5G10740.2 41.810 232 91 9 2 223 35 232 4.10e-38 135
Msa0662540 AT5G53140.1 38.496 226 107 7 2 223 103 300 7.91e-38 136
Msa0662540 AT5G53140.2 38.496 226 107 7 2 223 103 300 7.91e-38 136
Msa0662540 AT1G43900.1 41.228 228 97 9 2 223 125 321 2.73e-37 134
Msa0662540 AT4G31750.1 44.262 183 86 4 45 223 62 232 2.78e-37 132
Msa0662540 AT4G31750.3 44.262 183 86 4 45 223 62 232 2.78e-37 132
Msa0662540 AT4G31750.2 44.262 183 86 4 45 223 62 232 3.32e-37 132
Msa0662540 AT1G67820.2 41.899 179 92 4 47 223 115 283 3.43e-37 134
Msa0662540 AT1G07160.1 39.207 227 107 5 2 223 122 322 3.62e-37 134
Msa0662540 AT1G67820.1 41.899 179 92 4 47 223 151 319 6.73e-37 134
Msa0662540 AT2G30020.1 42.222 180 91 3 47 223 170 339 9.87e-37 133
Msa0662540 AT5G24940.1 41.379 232 92 10 2 223 35 232 1.06e-35 131
Msa0662540 AT2G40180.1 40.449 178 99 3 47 223 161 332 1.23e-32 122
Msa0662540 AT1G78200.5 34.653 202 111 6 31 223 1 190 1.60e-25 100
Msa0662540 AT1G78200.4 34.653 202 111 6 31 223 1 190 1.60e-25 100
Msa0662540 AT3G17250.1 33.654 208 116 7 32 223 141 342 2.39e-25 102
Msa0662540 AT1G78200.1 34.597 211 116 7 23 223 37 235 2.88e-25 100
Msa0662540 AT1G78200.3 34.597 211 116 7 23 223 37 235 2.88e-25 100
Msa0662540 AT1G78200.2 34.597 211 116 7 23 223 37 235 2.88e-25 100
Msa0662540 AT4G31860.2 32.593 270 113 11 2 223 25 273 1.06e-24 99.0
Msa0662540 AT1G22280.3 35.217 230 116 10 2 223 35 239 1.48e-24 98.6
Msa0662540 AT4G31860.3 32.593 270 113 11 2 223 25 273 1.63e-24 98.6
Msa0662540 AT4G31860.1 32.593 270 113 11 2 223 25 273 5.67e-24 98.2
Msa0662540 AT2G33700.1 36.757 185 95 7 47 223 123 293 1.89e-23 97.1
Msa0662540 AT1G48040.1 36.508 189 102 6 47 223 116 298 2.03e-23 97.1
Msa0662540 AT2G25070.1 30.258 271 120 11 1 223 24 273 2.05e-23 96.7
Msa0662540 AT2G25070.2 30.258 271 120 11 1 223 24 273 2.05e-23 96.7
Msa0662540 AT1G34750.4 38.378 185 96 6 44 223 63 234 2.83e-23 94.4
Msa0662540 AT1G22280.1 34.764 233 107 10 2 223 35 233 6.67e-23 94.4
Msa0662540 AT1G34750.2 38.378 185 96 6 44 223 63 234 6.86e-23 94.0
Msa0662540 AT1G34750.1 38.378 185 96 6 44 223 63 234 6.86e-23 94.0
Msa0662540 AT1G34750.3 38.378 185 96 6 44 223 63 234 6.86e-23 94.0
Msa0662540 AT3G51470.2 31.905 210 116 7 27 223 37 232 8.94e-23 94.4
Msa0662540 AT3G51470.1 31.905 210 116 7 27 223 81 276 1.75e-22 94.4
Msa0662540 AT2G25620.1 31.278 227 127 6 8 223 97 305 1.21e-21 92.4
Msa0662540 AT2G25620.2 31.278 227 127 6 8 223 97 305 1.21e-21 92.4
Msa0662540 AT2G40860.4 30.303 231 128 7 2 223 296 502 6.00e-21 91.3
Msa0662540 AT2G40860.2 30.303 231 128 7 2 223 394 600 6.46e-21 91.3
Msa0662540 AT2G40860.1 30.303 231 128 7 2 223 394 600 7.48e-21 90.9
Msa0662540 AT2G34740.3 35.000 200 109 7 32 223 97 283 7.50e-21 89.0
Msa0662540 AT2G34740.2 35.000 200 109 7 32 223 115 301 8.41e-21 89.4
Msa0662540 AT2G34740.1 35.000 200 109 7 32 223 101 287 1.27e-20 89.0
Msa0662540 AT3G62260.3 32.609 184 115 4 47 223 27 208 1.69e-20 87.8
Msa0662540 AT3G62260.2 30.374 214 130 5 27 223 87 298 2.66e-20 88.6
Msa0662540 AT3G62260.1 30.374 214 130 5 27 223 86 297 2.72e-20 88.6
Msa0662540 AT4G28400.2 33.333 198 115 6 32 223 49 235 3.53e-19 83.6
Msa0662540 AT4G28400.3 33.333 198 115 6 32 223 27 213 5.40e-19 83.2
Msa0662540 AT2G20630.1 32.000 200 115 6 32 223 45 231 6.48e-19 83.2
Msa0662540 AT4G28400.1 33.333 198 115 6 32 223 49 235 7.50e-19 83.2
Msa0662540 AT3G15260.2 36.216 185 100 6 44 223 70 241 1.05e-18 82.8
Msa0662540 AT3G15260.1 36.216 185 100 6 44 223 70 241 1.05e-18 82.8
Msa0662540 AT2G20630.2 32.000 200 115 6 32 223 45 231 1.13e-18 82.8
Msa0662540 AT2G40860.3 28.692 237 132 8 2 225 394 606 4.84e-18 82.8
Msa0662540 AT1G17545.1 34.532 139 49 3 22 155 77 178 3.83e-17 76.6
Msa0662540 AT4G08260.1 30.612 196 93 6 32 224 1 156 5.08e-17 77.0
Msa0662540 AT3G16800.2 32.195 205 112 8 45 223 91 294 3.20e-16 76.6
Msa0662540 AT3G16800.1 32.195 205 112 8 45 223 91 294 3.20e-16 76.6
Msa0662540 AT3G16800.3 32.195 205 112 8 45 223 91 294 3.20e-16 76.6
Msa0662540 AT5G26010.1 31.050 219 127 7 28 223 52 269 5.21e-16 75.9
Msa0662540 AT3G17090.2 31.925 213 104 8 47 223 84 291 1.12e-15 75.1
Msa0662540 AT1G68410.4 31.222 221 125 9 14 223 51 255 1.14e-15 75.5
Msa0662540 AT1G68410.3 31.222 221 125 9 14 223 51 255 1.14e-15 75.5
Msa0662540 AT1G68410.1 31.222 221 125 9 14 223 51 255 1.14e-15 75.5
Msa0662540 AT1G68410.2 31.222 221 125 9 14 223 51 255 1.14e-15 75.5
Msa0662540 AT3G17090.1 31.925 213 104 8 47 223 84 291 2.75e-15 74.3
Msa0662540 AT5G02760.1 28.761 226 114 6 36 223 61 277 3.43e-15 73.9
Msa0662540 AT3G16800.4 32.000 200 109 8 50 223 1 199 4.78e-15 72.4
Msa0662540 AT3G16800.6 32.000 200 109 8 50 223 1 199 4.78e-15 72.4
Msa0662540 AT1G09160.2 34.225 187 107 8 48 223 69 250 9.90e-15 72.8
Msa0662540 AT1G09160.1 34.225 187 107 8 48 223 69 250 9.90e-15 72.8
Msa0662540 AT5G27930.3 32.857 210 107 9 47 223 92 300 1.16e-14 72.0
Msa0662540 AT2G20050.2 27.700 213 110 6 46 223 142 345 1.67e-14 72.8
Msa0662540 AT2G20050.1 27.700 213 110 6 46 223 142 345 2.04e-14 72.4
Msa0662540 AT5G27930.1 32.857 210 107 9 47 223 92 300 2.39e-14 71.6
Msa0662540 AT5G27930.2 32.857 210 107 9 47 223 92 300 2.39e-14 71.6
Msa0662540 AT3G55050.1 27.386 241 127 7 26 223 56 291 3.11e-14 71.2
Msa0662540 AT3G55050.2 27.386 241 127 7 26 223 56 291 3.11e-14 71.2
Msa0662540 AT3G12620.2 28.241 216 116 6 43 223 79 290 4.15e-14 70.9
Msa0662540 AT3G12620.1 28.241 216 116 6 43 223 79 290 4.15e-14 70.9
Msa0662540 AT3G63320.2 34.194 155 56 5 115 223 84 238 4.18e-14 70.9
Msa0662540 AT3G63340.12 34.194 155 56 5 115 223 232 386 6.18e-14 70.9
Msa0662540 AT3G63340.11 34.194 155 56 5 115 223 232 386 7.46e-14 70.5
Msa0662540 AT3G63340.6 34.194 155 56 5 115 223 232 386 8.86e-14 70.5
Msa0662540 AT3G63340.3 34.194 155 56 5 115 223 232 386 8.86e-14 70.5
Msa0662540 AT3G63340.10 34.194 155 56 5 115 223 232 386 8.86e-14 70.5
Msa0662540 AT3G63340.4 34.194 155 56 5 115 223 232 386 8.86e-14 70.5
Msa0662540 AT3G63340.8 34.194 155 56 5 115 223 232 386 9.05e-14 70.5
Msa0662540 AT3G63340.5 34.194 155 56 5 115 223 232 386 9.26e-14 70.5
Msa0662540 AT3G63340.1 34.194 155 56 5 115 223 232 386 9.26e-14 70.5
Msa0662540 AT3G63340.7 34.194 155 56 5 115 223 232 386 9.80e-14 70.5
Msa0662540 AT3G63320.1 34.194 155 56 5 115 223 167 321 1.03e-13 70.1
Msa0662540 AT4G38520.2 28.378 222 122 7 36 223 70 288 1.35e-13 69.3
Msa0662540 AT4G38520.1 28.378 222 122 7 36 223 70 288 1.35e-13 69.3
Msa0662540 AT1G79630.2 28.629 248 106 8 47 223 28 275 2.48e-13 68.9
Msa0662540 AT1G79630.7 28.629 248 106 8 47 223 28 275 2.48e-13 68.9
Msa0662540 AT5G01700.1 31.553 206 112 9 47 223 29 234 3.00e-13 68.2
Msa0662540 AT1G79630.8 28.629 248 106 8 47 223 94 341 3.67e-13 68.6
Msa0662540 AT1G79630.1 28.629 248 106 8 47 223 98 345 3.93e-13 68.2
Msa0662540 AT1G18030.2 30.653 199 103 9 48 223 110 296 4.35e-13 67.4
Msa0662540 AT1G22280.2 32.804 189 84 9 2 181 35 189 4.92e-13 65.9
Msa0662540 AT1G18030.1 28.788 198 108 7 48 223 110 296 5.97e-13 67.4
Msa0662540 AT5G01700.2 31.553 206 112 9 47 223 78 283 6.89e-13 67.4
Msa0662540 AT1G47380.1 29.717 212 127 9 23 223 46 246 2.03e-12 66.2
Msa0662540 AT3G05640.2 32.524 206 107 9 47 223 93 295 2.80e-12 65.5
Msa0662540 AT3G05640.1 32.524 206 107 9 47 223 93 295 2.80e-12 65.5
Msa0662540 AT3G05640.3 32.524 206 107 9 47 223 93 295 2.80e-12 65.5
Msa0662540 AT3G63340.2 48.750 80 38 3 147 223 340 419 5.97e-12 65.1
Msa0662540 AT3G51370.1 27.602 221 111 8 43 223 75 286 8.15e-12 64.3
Msa0662540 AT3G51370.3 27.602 221 111 8 43 223 75 286 8.15e-12 64.3
Msa0662540 AT1G79630.6 34.694 147 76 5 96 223 90 235 1.71e-11 63.2
Msa0662540 AT1G79630.3 34.694 147 76 5 96 223 90 235 1.71e-11 63.2
Msa0662540 AT1G79630.4 34.694 147 76 5 96 223 90 235 1.71e-11 63.2
Msa0662540 AT1G79630.5 34.694 147 76 5 96 223 90 235 1.71e-11 63.2

Find 130 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAGAATAAAAGGCTAAAA+AGG 0.170785 4_4:-294587 Msa0662540:five_prime_UTR
TGTAATTGTGATAGATTTAA+AGG 0.171059 4_4:-292155 Msa0662540:three_prime_UTR
TGATGGGCATGGAGGCTTTC+AGG 0.182963 4_4:-293716 Msa0662540:intron
ATCAAATTTCCAAGTGTAAA+TGG 0.209870 4_4:+294455 None:intergenic
ACCATATATCATAGAATAAA+AGG 0.234400 4_4:-294596 Msa0662540:five_prime_UTR
GAAACAATTGGCAGGAAAAT+TGG 0.251738 4_4:-293527 Msa0662540:CDS
AGACTTAGGTTATTGCTAAA+AGG 0.251804 4_4:-294520 Msa0662540:five_prime_UTR
GTTAAAGACTAGAAGCTTAT+AGG 0.257111 4_4:-294550 Msa0662540:five_prime_UTR
CAAGGAAGCGAATCCTTCTT+TGG 0.267654 4_4:-292309 Msa0662540:three_prime_UTR
TCATTTATGCTATTGGTCTA+TGG 0.275482 4_4:-292079 Msa0662540:three_prime_UTR
AACCATCACTTGCTAAAATA+AGG 0.282020 4_4:+292370 None:intergenic
TGATTGAATGTGGGAGTAAT+TGG 0.283028 4_4:-294238 Msa0662540:five_prime_UTR
AAATATCTCATTTATGCTAT+TGG 0.288858 4_4:-292086 Msa0662540:three_prime_UTR
TATGTGGGTTTGATTTAAAA+TGG 0.290272 4_4:+293416 None:intergenic
TTTCAGTGCCTGCATAATTC+AGG 0.301475 4_4:-294155 Msa0662540:five_prime_UTR
CTTTGCACAATATTGACTTC+TGG 0.302547 4_4:+292411 None:intergenic
TACTCAATTCCAAACCATTC+TGG 0.307215 4_4:+293901 None:intergenic
AATTGAGTAGTGGCCCTCTA+TGG 0.307939 4_4:-293887 Msa0662540:exon
CGTCCTTTATAGCCTGGTTA+TGG 0.318628 4_4:+294201 None:intergenic
CTTCTTTGGCACAAGAAGTA+TGG 0.323327 4_4:-292295 Msa0662540:three_prime_UTR
TGAAGCTCGGCAGTCAAGTT+TGG 0.330887 4_4:-293564 Msa0662540:CDS
AGAAGAATAGCAGATAGTTT+TGG 0.339478 4_4:+293966 None:intergenic
TGGAAAGGATACAGAGTTCT+TGG 0.348321 4_4:-293166 Msa0662540:CDS
GATAACCGGGTTGAAGCTTA+TGG 0.383742 4_4:-294399 Msa0662540:five_prime_UTR
CACATAATTGTTGCAAATTG+TGG 0.384733 4_4:-293399 Msa0662540:CDS
CACGGTATCCGCGGAGGTAA+AGG 0.384992 4_4:-294297 Msa0662540:five_prime_UTR
TAGCATAAACGAGGTACTTC+CGG 0.395663 4_4:-294069 Msa0662540:five_prime_UTR
CACAATTTGCAACAATTATG+TGG 0.398808 4_4:+293400 None:intergenic
ACAACTCATATGGAGATAAC+CGG 0.401599 4_4:-294413 Msa0662540:five_prime_UTR
ACTTGGAAATTTGATTCAAA+AGG 0.416275 4_4:-294447 Msa0662540:five_prime_UTR
ACATAATTGTTGCAAATTGT+GGG 0.422260 4_4:-293398 Msa0662540:CDS
AAGCCATAACCAGGCTATAA+AGG 0.423467 4_4:-294204 Msa0662540:five_prime_UTR
TTGATATTAAATCCTGCAGT+TGG 0.427928 4_4:-294359 Msa0662540:five_prime_UTR
GCAGTGGAGCCAGCAGTCTC+AGG 0.434389 4_4:+293447 None:intergenic
TCACCAACCGTGACGATGAT+TGG 0.437340 4_4:+294110 None:intergenic
CGAGGTACTTCCGGCACTAA+AGG 0.437574 4_4:-294060 Msa0662540:five_prime_UTR
CATATCTAGTTTCTTTGTTG+AGG 0.438035 4_4:-295188 Msa0662540:five_prime_UTR
CTTGAAGATATTTCTTCCAT+TGG 0.442941 4_4:+294488 None:intergenic
TTAAAGACTAGAAGCTTATA+GGG 0.445492 4_4:-294549 Msa0662540:five_prime_UTR
TTCTGCTCGGCCTTTAGTGC+CGG 0.446492 4_4:+294050 None:intergenic
TTATAGGGTACTAGAGACTT+AGG 0.449944 4_4:-294534 Msa0662540:five_prime_UTR
TGCTGCTGTTACAACTCATA+TGG 0.453647 4_4:-294423 Msa0662540:five_prime_UTR
ATACAGAGTTCTTGGTGTCT+TGG 0.453913 4_4:-293158 Msa0662540:CDS
AACAAGCAACCAGAATGGTT+TGG 0.455011 4_4:-293910 Msa0662540:five_prime_UTR
ACAATATTGACTTCTGGTTC+TGG 0.455106 4_4:+292417 None:intergenic
AGTCCCTTCACAGATGCTAA+TGG 0.459379 4_4:-293794 Msa0662540:CDS
TCTGGTTCTGGAATTATCCA+TGG 0.460881 4_4:+292429 None:intergenic
CTCGCCCTCCACAGAGGAAG+CGG 0.461339 4_4:-292190 Msa0662540:three_prime_UTR
GAAGCAAATGGAGAAACAAT+TGG 0.462585 4_4:-293539 Msa0662540:CDS
ATTGAGTAGTGGCCCTCTAT+GGG 0.464718 4_4:-293886 Msa0662540:exon
CATACTTCTTGTGCCAAAGA+AGG 0.465903 4_4:+292296 None:intergenic
ATTATTATTATTATCTTCAA+AGG 0.477647 4_4:+295115 None:intergenic
TCTCATCGTCCTTTATAGCC+TGG 0.499525 4_4:+294195 None:intergenic
TTGGTGTCTTGGCCATGTCA+AGG 0.499546 4_4:-293147 Msa0662540:CDS
GCCCTCTCATCCTCTCGATT+CGG 0.503961 4_4:+293223 None:intergenic
TTGCAAATTGTGGGGATTCG+AGG 0.505415 4_4:-293389 Msa0662540:CDS
ACTAAAGGCCGAGCAGAATA+CGG 0.508870 4_4:-294045 Msa0662540:five_prime_UTR
CTGTGGAAATAGACCGGAGA+TGG 0.511631 4_4:-293851 Msa0662540:CDS
TCAAGTTTGGATGAAGCAAA+TGG 0.516274 4_4:-293551 Msa0662540:CDS
AGTTGGTGCGTCTGTTGTCT+CGG 0.520037 4_4:-294267 Msa0662540:five_prime_UTR
AAATCGAAGCGTGTCAGAAG+AGG 0.522032 4_4:-293994 Msa0662540:five_prime_UTR
ACGGTTGGTGATGAGATTCA+TGG 0.523608 4_4:-294098 Msa0662540:five_prime_UTR
GCAACAGCATCTTCCATCTC+CGG 0.529449 4_4:+293838 None:intergenic
ATCAATCTGTGGAAATAGAC+CGG 0.533852 4_4:-293857 Msa0662540:CDS
ACTATTGCTCCTGAGACTGC+TGG 0.535769 4_4:-293456 Msa0662540:CDS
TTTGGTGTCTATGATGGGCA+TGG 0.536239 4_4:-293727 Msa0662540:CDS
TCGAGGGCTGTCTTGTATCG+TGG 0.542408 4_4:-293372 Msa0662540:CDS
CGAGGGCTGTCTTGTATCGT+GGG 0.543022 4_4:-293371 Msa0662540:CDS
TTGGCCATGTCAAGGTCCAT+AGG 0.543817 4_4:-293139 Msa0662540:intron
TGCAAATTGTGGGGATTCGA+GGG 0.545554 4_4:-293388 Msa0662540:CDS
TTGAGAACAAGCAACCAGAA+TGG 0.553751 4_4:-293915 Msa0662540:five_prime_UTR
AGGGCAAGAATCGAAGCAGC+AGG 0.556886 4_4:-293205 Msa0662540:CDS
TATAAAATTCTAAGTGTACA+AGG 0.557288 4_4:-291984 Msa0662540:three_prime_UTR
ACAATTTGCAACAATTATGT+GGG 0.558102 4_4:+293401 None:intergenic
ATTGAGAGCCTGAATTATGC+AGG 0.559602 4_4:+294147 None:intergenic
TCTTCTTGATCAGTTGCCAA+AGG 0.560115 4_4:-293949 Msa0662540:five_prime_UTR
CATAATTGTTGCAAATTGTG+GGG 0.563435 4_4:-293397 Msa0662540:CDS
AAGAAGCATGTGATATTGCA+AGG 0.565187 4_4:-292327 Msa0662540:three_prime_UTR
GACTGCTGGCTCCACTGCAG+TGG 0.569359 4_4:-293442 Msa0662540:CDS
GAATACGGTTTCTGTTGCAA+TGG 0.570314 4_4:-294030 Msa0662540:five_prime_UTR
CAAATGGAGAAACAATTGGC+AGG 0.571400 4_4:-293535 Msa0662540:CDS
GTTGCAGTGCCATTTACACT+TGG 0.572388 4_4:-294464 Msa0662540:five_prime_UTR
GAGGTAAAGGAGAATCAAGT+TGG 0.572537 4_4:-294284 Msa0662540:five_prime_UTR
AACAGAAACCGTATTCTGCT+CGG 0.575565 4_4:+294037 None:intergenic
TCTACACTCAGCGTTGATTG+AGG 0.579519 4_4:-293591 Msa0662540:CDS
GCCGAATCGAGAGGATGAGA+GGG 0.580620 4_4:-293224 Msa0662540:CDS
CAGGAGGAAGAGTCATACAT+TGG 0.580820 4_4:-293186 Msa0662540:CDS
CTTGAGTGAAAGCCATAACC+AGG 0.583606 4_4:-294213 Msa0662540:five_prime_UTR
ATCGCCGCTTCCTCTGTGGA+GGG 0.585497 4_4:+292186 None:intergenic
GTGAATGAATGAATGAATGA+AGG 0.590232 4_4:+295154 None:intergenic
GTGATAGATACTTGAAACCA+TGG 0.595760 4_4:-292446 Msa0662540:CDS
TAAAATGGCAACCACTGCAG+TGG 0.596316 4_4:+293431 None:intergenic
CATACCTATGGACCTTGACA+TGG 0.597500 4_4:+293135 None:intergenic
TCGGAAATGTTGATTGAATG+TGG 0.599725 4_4:-294248 Msa0662540:five_prime_UTR
TATCGCCGCTTCCTCTGTGG+AGG 0.600276 4_4:+292185 None:intergenic
CGATGAGATCATGTTAGCTG+TGG 0.601298 4_4:-294180 Msa0662540:five_prime_UTR
AGCCGAATCGAGAGGATGAG+AGG 0.601648 4_4:-293225 Msa0662540:CDS
TGTTATCGCCGCTTCCTCTG+TGG 0.606503 4_4:+292182 None:intergenic
CAACTCATATGGAGATAACC+GGG 0.606916 4_4:-294412 Msa0662540:five_prime_UTR
GTGGTCAGAAGACAACGCTA+TGG 0.607535 4_4:+293341 None:intergenic
ACAGTACTTGACAAAGGAGA+AGG 0.608657 4_4:-292259 Msa0662540:three_prime_UTR
TCATCCATTAGCATCTGTGA+AGG 0.613517 4_4:+293790 None:intergenic
TAAAAGGCAAAGTTAACCAA+TGG 0.614252 4_4:-294504 Msa0662540:five_prime_UTR
ATAATAATAATAATCAACTC+AGG 0.614565 4_4:-295094 Msa0662540:intron
TCACCAATCATCGTCACGGT+TGG 0.616215 4_4:-294113 Msa0662540:five_prime_UTR
GGAAGAGTCATACATTGGAA+AGG 0.617081 4_4:-293181 Msa0662540:CDS
GATTGATGAGAAACCCATAG+AGG 0.620457 4_4:+293873 None:intergenic
ATGGGTTTCTCATCAATCTG+TGG 0.620478 4_4:-293868 Msa0662540:CDS
CATCCATTAGCATCTGTGAA+GGG 0.623872 4_4:+293791 None:intergenic
TAATTCAGGCTCTCAATCTG+TGG 0.624297 4_4:-294141 Msa0662540:five_prime_UTR
ATTGATGAGAAACCCATAGA+GGG 0.628750 4_4:+293874 None:intergenic
AATGGTTTGGAATTGAGTAG+TGG 0.634780 4_4:-293897 Msa0662540:five_prime_UTR
CGGAAATGTTGATTGAATGT+GGG 0.636981 4_4:-294247 Msa0662540:five_prime_UTR
GTACTTGACAAAGGAGAAGG+AGG 0.638743 4_4:-292256 Msa0662540:three_prime_UTR
AATGAATGAATGAATGAAGG+AGG 0.639567 4_4:+295157 None:intergenic
GCAAGAATCGAAGCAGCAGG+AGG 0.645246 4_4:-293202 Msa0662540:CDS
GTTGAAGACTAACATCAGCA+TGG 0.646810 4_4:+294327 None:intergenic
TCAGATCACGGTATCCGCGG+AGG 0.649352 4_4:-294303 Msa0662540:five_prime_UTR
TGATTGAGGAGATTGAAGCT+CGG 0.650060 4_4:-293577 Msa0662540:CDS
ATTTGCCATAAGCTTCAACC+CGG 0.654249 4_4:+294394 None:intergenic
CACAAGAAGTATGGTGACAA+CGG 0.654302 4_4:-292286 Msa0662540:three_prime_UTR
ACATCAGATCACGGTATCCG+CGG 0.656186 4_4:-294306 Msa0662540:five_prime_UTR
AGATTCACCAATCATCGTCA+CGG 0.661305 4_4:-294117 Msa0662540:five_prime_UTR
TAGTCTTCAACATCAGATCA+CGG 0.669945 4_4:-294315 Msa0662540:five_prime_UTR
TTGATTCTCCTTTACCTCCG+CGG 0.675182 4_4:+294289 None:intergenic
GGTGTCTATGATGGGCATGG+AGG 0.675725 4_4:-293724 Msa0662540:CDS
GGAGCAACAGTACTTGACAA+AGG 0.702207 4_4:-292265 Msa0662540:three_prime_UTR
TCTAAACTCGCCCTCCACAG+AGG 0.709645 4_4:-292196 Msa0662540:three_prime_UTR
TGGTAAGTTTAGCATAAACG+AGG 0.709809 4_4:-294078 Msa0662540:five_prime_UTR
GAGTTTAGATAGATACTCTG+CGG 0.743383 4_4:+292210 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATTATTATCTTCAA+AGG + chr4_4:292089-292108 None:intergenic 10.0%
!! TTCAATTAAAAAAAAATTAG+AGG - chr4_4:295275-295294 Msa0662540:five_prime_UTR 10.0%
!! TAAAAATAAACAATTCTTGA+AGG - chr4_4:291958-291977 Msa0662540:three_prime_UTR 15.0%
!! ATAATAATAATAATCAACTC+AGG - chr4_4:292107-292126 Msa0662540:three_prime_UTR 15.0%
!! ATAATATTATTTGCCTTAAA+GGG - chr4_4:292176-292195 Msa0662540:three_prime_UTR 15.0%
!!! TTTTAAGTTTTAAGAAAGAA+AGG - chr4_4:294270-294289 Msa0662540:five_prime_UTR 15.0%
!! CATAATATTATTTGCCTTAA+AGG - chr4_4:292175-292194 Msa0662540:three_prime_UTR 20.0%
!!! CTGTGAATTTTGTTTATAAT+TGG - chr4_4:292334-292353 Msa0662540:three_prime_UTR 20.0%
!! ACCATATATCATAGAATAAA+AGG - chr4_4:292605-292624 Msa0662540:intron 20.0%
!! ATAGAATAAAAGGCTAAAAA+GGG - chr4_4:292615-292634 Msa0662540:intron 20.0%
!!! ATTTTGTGTTTTCATTCAAA+TGG + chr4_4:293437-293456 None:intergenic 20.0%
!! CAAATTGTATGATTTGATTA+AGG + chr4_4:293517-293536 None:intergenic 20.0%
!!! ACAATTGTTAGTTTATTGAT+AGG - chr4_4:294308-294327 Msa0662540:five_prime_UTR 20.0%
!! AAAAATATTATAGTGCATCA+TGG - chr4_4:294557-294576 Msa0662540:five_prime_UTR 20.0%
!! ATTAATCTCATTACTGATAT+GGG + chr4_4:294711-294730 None:intergenic 20.0%
!! AATTAATCTCATTACTGATA+TGG + chr4_4:294712-294731 None:intergenic 20.0%
!! TGTAATTGTGATAGATTTAA+AGG - chr4_4:295046-295065 Msa0662540:intron 20.0%
!! AAATATCTCATTTATGCTAT+TGG - chr4_4:295115-295134 Msa0662540:five_prime_UTR 20.0%
!! TATAAAATTCTAAGTGTACA+AGG - chr4_4:295217-295236 Msa0662540:five_prime_UTR 20.0%
! TGAAAGGAAACAAAATCAAT+AGG + chr4_4:292138-292157 None:intergenic 25.0%
! TTTCAACTATAAAGAGTGAA+AGG + chr4_4:292154-292173 None:intergenic 25.0%
! ATCAAGGATAAGAAATTTCA+TGG - chr4_4:292246-292265 Msa0662540:three_prime_UTR 25.0%
! AACAAAATTCACAGAAAACA+AGG + chr4_4:292328-292347 None:intergenic 25.0%
! GTCTTTATGATGATTTCTAA+AGG - chr4_4:292422-292441 Msa0662540:CDS 25.0%
!!! ATTTGATTTGATGTACAAAG+AGG - chr4_4:292469-292488 Msa0662540:intron 25.0%
!!! ACACTTATTTGACTTTTAAG+AGG - chr4_4:292517-292536 Msa0662540:intron 25.0%
!! GCCTTTTATTCTATGATATA+TGG + chr4_4:292609-292628 None:intergenic 25.0%
! CATAGAATAAAAGGCTAAAA+AGG - chr4_4:292614-292633 Msa0662540:intron 25.0%
! TAGAATAAAAGGCTAAAAAG+GGG - chr4_4:292616-292635 Msa0662540:intron 25.0%
! TTAAAGACTAGAAGCTTATA+GGG - chr4_4:292652-292671 Msa0662540:intron 25.0%
! ATCAAATTTCCAAGTGTAAA+TGG + chr4_4:292749-292768 None:intergenic 25.0%
! ACTTGGAAATTTGATTCAAA+AGG - chr4_4:292754-292773 Msa0662540:intron 25.0%
!!! ATTTTTTTGGTGTCTATGAT+GGG - chr4_4:293469-293488 Msa0662540:CDS 25.0%
!! TATGTGGGTTTGATTTAAAA+TGG + chr4_4:293788-293807 None:intergenic 25.0%
! ACAATTTGCAACAATTATGT+GGG + chr4_4:293803-293822 None:intergenic 25.0%
! ACATAATTGTTGCAAATTGT+GGG - chr4_4:293803-293822 Msa0662540:CDS 25.0%
! TCTATTCAGTTTGGTATTAA+GGG - chr4_4:294145-294164 Msa0662540:five_prime_UTR 25.0%
!! TTCTTTTAATCAGTATCTCA+AGG - chr4_4:294345-294364 Msa0662540:five_prime_UTR 25.0%
!! TTTTTCTGTTCCAAAGTTAT+GGG + chr4_4:294372-294391 None:intergenic 25.0%
! AAAGAATATATCCTATCCAA+AGG + chr4_4:294428-294447 None:intergenic 25.0%
! AGTTTCCTTTATTCAGAAAA+TGG - chr4_4:294582-294601 Msa0662540:five_prime_UTR 25.0%
!!! TTAAGCCATTTTCTGAATAA+AGG + chr4_4:294590-294609 None:intergenic 25.0%
! AACAAGTTTATGATATCCAA+AGG + chr4_4:294638-294657 None:intergenic 25.0%
! TTAATCTCATTACTGATATG+GGG + chr4_4:294710-294729 None:intergenic 25.0%
! CATATCTAGTTTCTTTGTTG+AGG - chr4_4:292013-292032 Msa0662540:three_prime_UTR 30.0%
AATGAATGAATGAATGAAGG+AGG + chr4_4:292047-292066 None:intergenic 30.0%
GTGAATGAATGAATGAATGA+AGG + chr4_4:292050-292069 None:intergenic 30.0%
TGTTGATCATGTGATATGAA+AGG - chr4_4:292209-292228 Msa0662540:three_prime_UTR 30.0%
GTGATATGAAAGGAACAATT+TGG - chr4_4:292219-292238 Msa0662540:three_prime_UTR 30.0%
GATACAAAAAAGAGAGAGAA+CGG + chr4_4:292301-292320 None:intergenic 30.0%
TCTAAAGGATCTTGTATTGT+AGG - chr4_4:292437-292456 Msa0662540:CDS 30.0%
AAACATCAAGAATTCTCCTA+CGG + chr4_4:292571-292590 None:intergenic 30.0%
GTTAAAGACTAGAAGCTTAT+AGG - chr4_4:292651-292670 Msa0662540:intron 30.0%
AGACTTAGGTTATTGCTAAA+AGG - chr4_4:292681-292700 Msa0662540:intron 30.0%
TAAAAGGCAAAGTTAACCAA+TGG - chr4_4:292697-292716 Msa0662540:intron 30.0%
CTTGAAGATATTTCTTCCAT+TGG + chr4_4:292716-292735 None:intergenic 30.0%
TTGATATTAAATCCTGCAGT+TGG - chr4_4:292842-292861 Msa0662540:intron 30.0%
! AGAAGAATAGCAGATAGTTT+TGG + chr4_4:293238-293257 None:intergenic 30.0%
!!! TATCCACAAGTCCATTTTTT+TGG - chr4_4:293456-293475 Msa0662540:CDS 30.0%
CATCATAGACACCAAAAAAA+TGG + chr4_4:293470-293489 None:intergenic 30.0%
!!! CATTTTTTTGGTGTCTATGA+TGG - chr4_4:293468-293487 Msa0662540:CDS 30.0%
CACAATTTGCAACAATTATG+TGG + chr4_4:293804-293823 None:intergenic 30.0%
CACATAATTGTTGCAAATTG+TGG - chr4_4:293802-293821 Msa0662540:CDS 30.0%
CATAATTGTTGCAAATTGTG+GGG - chr4_4:293804-293823 Msa0662540:CDS 30.0%
CTCTATTCAGTTTGGTATTA+AGG - chr4_4:294144-294163 Msa0662540:five_prime_UTR 30.0%
CTATTCAGTTTGGTATTAAG+GGG - chr4_4:294146-294165 Msa0662540:five_prime_UTR 30.0%
!!! TCAAACGTTTTTTGCTGAAT+AGG - chr4_4:294192-294211 Msa0662540:five_prime_UTR 30.0%
!!! CAAACGTTTTTTGCTGAATA+GGG - chr4_4:294193-294212 Msa0662540:five_prime_UTR 30.0%
AGTTTATTGATAGGAATTCG+TGG - chr4_4:294317-294336 Msa0662540:five_prime_UTR 30.0%
! CTTTTTCTGTTCCAAAGTTA+TGG + chr4_4:294373-294392 None:intergenic 30.0%
!! TGCAGTTTTTAACTCAAACA+AGG + chr4_4:294678-294697 None:intergenic 30.0%
! TCTTATTGTCTTGTTCAAAC+AGG - chr4_4:294733-294752 Msa0662540:intron 30.0%
! AACCATCACTTGCTAAAATA+AGG + chr4_4:294834-294853 None:intergenic 30.0%
!! ATTTTAGCAAGTGATGGTTA+TGG - chr4_4:294835-294854 Msa0662540:intron 30.0%
! TTTTAGCAAGTGATGGTTAT+GGG - chr4_4:294836-294855 Msa0662540:intron 30.0%
! TCATTTATGCTATTGGTCTA+TGG - chr4_4:295122-295141 Msa0662540:five_prime_UTR 30.0%
! ATTGAAACTTTTGTGATGCT+TGG + chr4_4:295261-295280 None:intergenic 30.0%
ACATGATGAATCACCCTTTA+AGG + chr4_4:292192-292211 None:intergenic 35.0%
GGAACAATTTGGTCAAATCA+AGG - chr4_4:292230-292249 Msa0662540:three_prime_UTR 35.0%
AGGATAAGAAATTTCATGGC+CGG - chr4_4:292250-292269 Msa0662540:three_prime_UTR 35.0%
AACCTTCTAGCAGATAAATC+AGG + chr4_4:292386-292405 None:intergenic 35.0%
CCTACGGCAAGAAATTTAAA+GGG + chr4_4:292555-292574 None:intergenic 35.0%
TCCTACGGCAAGAAATTTAA+AGG + chr4_4:292556-292575 None:intergenic 35.0%
AATAAAAGGCTAAAAAGGGG+AGG - chr4_4:292619-292638 Msa0662540:intron 35.0%
TAAAAAGGGGAGGAAAAAAG+TGG - chr4_4:292629-292648 Msa0662540:intron 35.0%
! TTATAGGGTACTAGAGACTT+AGG - chr4_4:292667-292686 Msa0662540:intron 35.0%
ACAACTCATATGGAGATAAC+CGG - chr4_4:292788-292807 Msa0662540:intron 35.0%
TAGTCTTCAACATCAGATCA+CGG - chr4_4:292886-292905 Msa0662540:intron 35.0%
! TCGGAAATGTTGATTGAATG+TGG - chr4_4:292953-292972 Msa0662540:intron 35.0%
! CGGAAATGTTGATTGAATGT+GGG - chr4_4:292954-292973 Msa0662540:intron 35.0%
TGATTGAATGTGGGAGTAAT+TGG - chr4_4:292963-292982 Msa0662540:intron 35.0%
TGGTAAGTTTAGCATAAACG+AGG - chr4_4:293123-293142 Msa0662540:intron 35.0%
!! CTCAATGTTTTGTTCTCCTT+TGG + chr4_4:293271-293290 None:intergenic 35.0%
TACTCAATTCCAAACCATTC+TGG + chr4_4:293303-293322 None:intergenic 35.0%
! AATGGTTTGGAATTGAGTAG+TGG - chr4_4:293304-293323 Msa0662540:intron 35.0%
ATTGATGAGAAACCCATAGA+GGG + chr4_4:293330-293349 None:intergenic 35.0%
ATCAATCTGTGGAAATAGAC+CGG - chr4_4:293344-293363 Msa0662540:CDS 35.0%
! ACACCAAAAAAATGGACTTG+TGG + chr4_4:293462-293481 None:intergenic 35.0%
!!! GAGAGTGTTTTGTTTCTGAT+AGG - chr4_4:293565-293584 Msa0662540:CDS 35.0%
TCAAGTTTGGATGAAGCAAA+TGG - chr4_4:293650-293669 Msa0662540:intron 35.0%
GAAGCAAATGGAGAAACAAT+TGG - chr4_4:293662-293681 Msa0662540:intron 35.0%
GAAACAATTGGCAGGAAAAT+TGG - chr4_4:293674-293693 Msa0662540:intron 35.0%
CTATGAAATGCCTTACTTTG+TGG + chr4_4:293882-293901 None:intergenic 35.0%
ATGCAACAGATACATACCTA+TGG + chr4_4:294081-294100 None:intergenic 35.0%
GTATGTATCTGTTGCATGAT+TGG - chr4_4:294084-294103 Msa0662540:five_prime_UTR 35.0%
GCATGTTACTCTATTCAGTT+TGG - chr4_4:294136-294155 Msa0662540:five_prime_UTR 35.0%
AAAGGCTGAAATCACATTCA+AGG + chr4_4:294410-294429 None:intergenic 35.0%
TTGAATGTGATTTCAGCCTT+TGG - chr4_4:294409-294428 Msa0662540:five_prime_UTR 35.0%
AAAGGCTGAAATCACATTCA+AGG + chr4_4:294410-294429 None:intergenic 35.0%
TTGAATGTGATTTCAGCCTT+TGG - chr4_4:294409-294428 Msa0662540:five_prime_UTR 35.0%
GTGATAGATACTTGAAACCA+TGG - chr4_4:294755-294774 Msa0662540:intron 35.0%
!! ACAATATTGACTTCTGGTTC+TGG + chr4_4:294787-294806 None:intergenic 35.0%
!! CTTTGCACAATATTGACTTC+TGG + chr4_4:294793-294812 None:intergenic 35.0%
!!! TGCCTTATTTTAGCAAGTGA+TGG - chr4_4:294829-294848 Msa0662540:intron 35.0%
! AAGAAGCATGTGATATTGCA+AGG - chr4_4:294874-294893 Msa0662540:intron 35.0%
GAGTTTAGATAGATACTCTG+CGG + chr4_4:294994-295013 None:intergenic 35.0%
AGCCCTTTGTGAAAAATTGA+GGG + chr4_4:295180-295199 None:intergenic 35.0%
TAGCCCTTTGTGAAAAATTG+AGG + chr4_4:295181-295200 None:intergenic 35.0%
! AACTTTTGTGATGCTTGGAA+AGG + chr4_4:295256-295275 None:intergenic 35.0%
CAAAGGATGAAAAACAGTGC+AGG + chr4_4:292072-292091 None:intergenic 40.0%
! TGTGACTTGTCTCAGATCTA+GGG - chr4_4:292273-292292 Msa0662540:three_prime_UTR 40.0%
TGCCTGATTTATCTGCTAGA+AGG - chr4_4:292381-292400 Msa0662540:CDS 40.0%
CCCTTTAAATTTCTTGCCGT+AGG - chr4_4:292552-292571 Msa0662540:intron 40.0%
TGCTGCTGTTACAACTCATA+TGG - chr4_4:292778-292797 Msa0662540:intron 40.0%
CAACTCATATGGAGATAACC+GGG - chr4_4:292789-292808 Msa0662540:intron 40.0%
ATTTGCCATAAGCTTCAACC+CGG + chr4_4:292810-292829 None:intergenic 40.0%
! TGGTTTTCATTTCCAACTGC+AGG + chr4_4:292857-292876 None:intergenic 40.0%
GTTGAAGACTAACATCAGCA+TGG + chr4_4:292877-292896 None:intergenic 40.0%
GAGGTAAAGGAGAATCAAGT+TGG - chr4_4:292917-292936 Msa0662540:intron 40.0%
AAGCCATAACCAGGCTATAA+AGG - chr4_4:292997-293016 Msa0662540:intron 40.0%
TTTCAGTGCCTGCATAATTC+AGG - chr4_4:293046-293065 Msa0662540:intron 40.0%
ATTGAGAGCCTGAATTATGC+AGG + chr4_4:293057-293076 None:intergenic 40.0%
TAATTCAGGCTCTCAATCTG+TGG - chr4_4:293060-293079 Msa0662540:intron 40.0%
AGATTCACCAATCATCGTCA+CGG - chr4_4:293084-293103 Msa0662540:intron 40.0%
! TAGCATAAACGAGGTACTTC+CGG - chr4_4:293132-293151 Msa0662540:intron 40.0%
AACAGAAACCGTATTCTGCT+CGG + chr4_4:293167-293186 None:intergenic 40.0%
GAATACGGTTTCTGTTGCAA+TGG - chr4_4:293171-293190 Msa0662540:CDS 40.0%
TCTTCTTGATCAGTTGCCAA+AGG - chr4_4:293252-293271 Msa0662540:intron 40.0%
TTGAGAACAAGCAACCAGAA+TGG - chr4_4:293286-293305 Msa0662540:intron 40.0%
AACAAGCAACCAGAATGGTT+TGG - chr4_4:293291-293310 Msa0662540:intron 40.0%
GATTGATGAGAAACCCATAG+AGG + chr4_4:293331-293350 None:intergenic 40.0%
ATGGGTTTCTCATCAATCTG+TGG - chr4_4:293333-293352 Msa0662540:intron 40.0%
CATCCATTAGCATCTGTGAA+GGG + chr4_4:293413-293432 None:intergenic 40.0%
TCATCCATTAGCATCTGTGA+AGG + chr4_4:293414-293433 None:intergenic 40.0%
TGATTGAGGAGATTGAAGCT+CGG - chr4_4:293624-293643 Msa0662540:intron 40.0%
CAAATGGAGAAACAATTGGC+AGG - chr4_4:293666-293685 Msa0662540:intron 40.0%
TGTCTTCTGACCACAAAGTA+AGG - chr4_4:293869-293888 Msa0662540:CDS 40.0%
ATTATGTCAGACGCTGAAGT+TGG - chr4_4:293923-293942 Msa0662540:five_prime_UTR 40.0%
GGAAGAGTCATACATTGGAA+AGG - chr4_4:294020-294039 Msa0662540:five_prime_UTR 40.0%
TGGAAAGGATACAGAGTTCT+TGG - chr4_4:294035-294054 Msa0662540:five_prime_UTR 40.0%
!! ATACAGAGTTCTTGGTGTCT+TGG - chr4_4:294043-294062 Msa0662540:five_prime_UTR 40.0%
! TTTTTGCTGAATAGGGACAC+AGG - chr4_4:294200-294219 Msa0662540:five_prime_UTR 40.0%
ATCTCAAGGACCCATAACTT+TGG - chr4_4:294359-294378 Msa0662540:five_prime_UTR 40.0%
TGTGATTTCAGCCTTTGGAT+AGG - chr4_4:294414-294433 Msa0662540:five_prime_UTR 40.0%
!! TCTGGTTCTGGAATTATCCA+TGG + chr4_4:294775-294794 None:intergenic 40.0%
CATACTTCTTGTGCCAAAGA+AGG + chr4_4:294908-294927 None:intergenic 40.0%
CTTCTTTGGCACAAGAAGTA+TGG - chr4_4:294906-294925 Msa0662540:intron 40.0%
! CACAAGAAGTATGGTGACAA+CGG - chr4_4:294915-294934 Msa0662540:intron 40.0%
ACAGTACTTGACAAAGGAGA+AGG - chr4_4:294942-294961 Msa0662540:intron 40.0%
! AGCCCCTCAATTTTTCACAA+AGG - chr4_4:295174-295193 Msa0662540:five_prime_UTR 40.0%
! GCCCCTCAATTTTTCACAAA+GGG - chr4_4:295175-295194 Msa0662540:five_prime_UTR 40.0%
GCCCTTTGTGAAAAATTGAG+GGG + chr4_4:295179-295198 None:intergenic 40.0%
! AGATCTGAGACAAGTCACAC+CGG + chr4_4:292272-292291 None:intergenic 45.0%
! GTGTGACTTGTCTCAGATCT+AGG - chr4_4:292272-292291 Msa0662540:three_prime_UTR 45.0%
!! GTTGCAGTGCCATTTACACT+TGG - chr4_4:292737-292756 Msa0662540:intron 45.0%
! GATAACCGGGTTGAAGCTTA+TGG - chr4_4:292802-292821 Msa0662540:intron 45.0%
TTGATTCTCCTTTACCTCCG+CGG + chr4_4:292915-292934 None:intergenic 45.0%
CTTGAGTGAAAGCCATAACC+AGG - chr4_4:292988-293007 Msa0662540:intron 45.0%
CGTCCTTTATAGCCTGGTTA+TGG + chr4_4:293003-293022 None:intergenic 45.0%
TCTCATCGTCCTTTATAGCC+TGG + chr4_4:293009-293028 None:intergenic 45.0%
CGATGAGATCATGTTAGCTG+TGG - chr4_4:293021-293040 Msa0662540:intron 45.0%
!! ACGGTTGGTGATGAGATTCA+TGG - chr4_4:293103-293122 Msa0662540:intron 45.0%
ACTAAAGGCCGAGCAGAATA+CGG - chr4_4:293156-293175 Msa0662540:CDS 45.0%
AAATCGAAGCGTGTCAGAAG+AGG - chr4_4:293207-293226 Msa0662540:CDS 45.0%
AATTGAGTAGTGGCCCTCTA+TGG - chr4_4:293314-293333 Msa0662540:intron 45.0%
ATTGAGTAGTGGCCCTCTAT+GGG - chr4_4:293315-293334 Msa0662540:intron 45.0%
GAAGGGACTTGAAAAAGCTG+AGG + chr4_4:293396-293415 None:intergenic 45.0%
AGTCCCTTCACAGATGCTAA+TGG - chr4_4:293407-293426 Msa0662540:CDS 45.0%
!! TTTGGTGTCTATGATGGGCA+TGG - chr4_4:293474-293493 Msa0662540:CDS 45.0%
!! TCTACACTCAGCGTTGATTG+AGG - chr4_4:293610-293629 Msa0662540:CDS 45.0%
TAAAATGGCAACCACTGCAG+TGG + chr4_4:293773-293792 None:intergenic 45.0%
TTGCAAATTGTGGGGATTCG+AGG - chr4_4:293812-293831 Msa0662540:CDS 45.0%
TGCAAATTGTGGGGATTCGA+GGG - chr4_4:293813-293832 Msa0662540:CDS 45.0%
CAGGAGGAAGAGTCATACAT+TGG - chr4_4:294015-294034 Msa0662540:five_prime_UTR 45.0%
CATACCTATGGACCTTGACA+TGG + chr4_4:294069-294088 None:intergenic 45.0%
CAAGGAAGCGAATCCTTCTT+TGG - chr4_4:294892-294911 Msa0662540:intron 45.0%
GGAGCAACAGTACTTGACAA+AGG - chr4_4:294936-294955 Msa0662540:intron 45.0%
GTACTTGACAAAGGAGAAGG+AGG - chr4_4:294945-294964 Msa0662540:intron 45.0%
ACATCAGATCACGGTATCCG+CGG - chr4_4:292895-292914 Msa0662540:intron 50.0%
! AGTTGGTGCGTCTGTTGTCT+CGG - chr4_4:292934-292953 Msa0662540:intron 50.0%
TCACCAATCATCGTCACGGT+TGG - chr4_4:293088-293107 Msa0662540:intron 50.0%
TCACCAACCGTGACGATGAT+TGG + chr4_4:293094-293113 None:intergenic 50.0%
CTGTGGAAATAGACCGGAGA+TGG - chr4_4:293350-293369 Msa0662540:CDS 50.0%
GCAACAGCATCTTCCATCTC+CGG + chr4_4:293366-293385 None:intergenic 50.0%
TGAAGCTCGGCAGTCAAGTT+TGG - chr4_4:293637-293656 Msa0662540:intron 50.0%
ACTATTGCTCCTGAGACTGC+TGG - chr4_4:293745-293764 Msa0662540:CDS 50.0%
GTGGTCAGAAGACAACGCTA+TGG + chr4_4:293863-293882 None:intergenic 50.0%
! ACTTTTGCAGCCGAATCGAG+AGG - chr4_4:293968-293987 Msa0662540:five_prime_UTR 50.0%
! TTGGTGTCTTGGCCATGTCA+AGG - chr4_4:294054-294073 Msa0662540:five_prime_UTR 50.0%
TTGGCCATGTCAAGGTCCAT+AGG - chr4_4:294062-294081 Msa0662540:five_prime_UTR 50.0%
! CGAGGTACTTCCGGCACTAA+AGG - chr4_4:293141-293160 Msa0662540:CDS 55.0%
TTCTGCTCGGCCTTTAGTGC+CGG + chr4_4:293154-293173 None:intergenic 55.0%
!! GGTGTCTATGATGGGCATGG+AGG - chr4_4:293477-293496 Msa0662540:CDS 55.0%
! TGATGGGCATGGAGGCTTTC+AGG - chr4_4:293485-293504 Msa0662540:intron 55.0%
TCGAGGGCTGTCTTGTATCG+TGG - chr4_4:293829-293848 Msa0662540:CDS 55.0%
CGAGGGCTGTCTTGTATCGT+GGG - chr4_4:293830-293849 Msa0662540:CDS 55.0%
AGCCGAATCGAGAGGATGAG+AGG - chr4_4:293976-293995 Msa0662540:five_prime_UTR 55.0%
GCCGAATCGAGAGGATGAGA+GGG - chr4_4:293977-293996 Msa0662540:five_prime_UTR 55.0%
GCCCTCTCATCCTCTCGATT+CGG + chr4_4:293981-294000 None:intergenic 55.0%
AGGGCAAGAATCGAAGCAGC+AGG - chr4_4:293996-294015 Msa0662540:five_prime_UTR 55.0%
GCAAGAATCGAAGCAGCAGG+AGG - chr4_4:293999-294018 Msa0662540:five_prime_UTR 55.0%
TCTAAACTCGCCCTCCACAG+AGG - chr4_4:295005-295024 Msa0662540:intron 55.0%
TGTTATCGCCGCTTCCTCTG+TGG + chr4_4:295022-295041 None:intergenic 55.0%
TCAGATCACGGTATCCGCGG+AGG - chr4_4:292898-292917 Msa0662540:intron 60.0%
CACGGTATCCGCGGAGGTAA+AGG - chr4_4:292904-292923 Msa0662540:intron 60.0%
ATCGCCGCTTCCTCTGTGGA+GGG + chr4_4:295018-295037 None:intergenic 60.0%
TATCGCCGCTTCCTCTGTGG+AGG + chr4_4:295019-295038 None:intergenic 60.0%
GCAGTGGAGCCAGCAGTCTC+AGG + chr4_4:293757-293776 None:intergenic 65.0%
!! GACTGCTGGCTCCACTGCAG+TGG - chr4_4:293759-293778 Msa0662540:CDS 65.0%
CTCGCCCTCCACAGAGGAAG+CGG - chr4_4:295011-295030 Msa0662540:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 291925 295298 291925 ID=Msa0662540;Name=Msa0662540
chr4_4 mRNA 291925 295298 291925 ID=Msa0662540-mRNA-1;Parent=Msa0662540;Name=Msa0662540-mRNA-1;_AED=0.03;_eAED=0.03;_QI=963|0.8|0.66|1|0.2|0.33|6|434|226
chr4_4 exon 291925 292468 291925 ID=Msa0662540-mRNA-1:exon:146;Parent=Msa0662540-mRNA-1
chr4_4 exon 293140 293245 293140 ID=Msa0662540-mRNA-1:exon:145;Parent=Msa0662540-mRNA-1
chr4_4 exon 293338 293499 293338 ID=Msa0662540-mRNA-1:exon:144;Parent=Msa0662540-mRNA-1
chr4_4 exon 293508 293636 293508 ID=Msa0662540-mRNA-1:exon:143;Parent=Msa0662540-mRNA-1
chr4_4 exon 293717 294649 293717 ID=Msa0662540-mRNA-1:exon:142;Parent=Msa0662540-mRNA-1
chr4_4 exon 295095 295298 295095 ID=Msa0662540-mRNA-1:exon:141;Parent=Msa0662540-mRNA-1
chr4_4 five_prime_UTR 295095 295298 295095 ID=Msa0662540-mRNA-1:five_prime_utr;Parent=Msa0662540-mRNA-1
chr4_4 five_prime_UTR 293891 294649 293891 ID=Msa0662540-mRNA-1:five_prime_utr;Parent=Msa0662540-mRNA-1
chr4_4 CDS 293717 293890 293717 ID=Msa0662540-mRNA-1:cds;Parent=Msa0662540-mRNA-1
chr4_4 CDS 293508 293636 293508 ID=Msa0662540-mRNA-1:cds;Parent=Msa0662540-mRNA-1
chr4_4 CDS 293338 293499 293338 ID=Msa0662540-mRNA-1:cds;Parent=Msa0662540-mRNA-1
chr4_4 CDS 293140 293245 293140 ID=Msa0662540-mRNA-1:cds;Parent=Msa0662540-mRNA-1
chr4_4 CDS 292359 292468 292359 ID=Msa0662540-mRNA-1:cds;Parent=Msa0662540-mRNA-1
chr4_4 three_prime_UTR 291925 292358 291925 ID=Msa0662540-mRNA-1:three_prime_utr;Parent=Msa0662540-mRNA-1
Gene Sequence

>Msa0662540

ATGGGTTTCTCATCAATCTGTGGAAATAGACCGGAGATGGAAGATGCTGTTGCTGTCAAACCTCAGCTTTTTCAAGTCCCTTCACAGATGCTAATGGATGACCATTTGAATGAAAACACAAAATATCCACAAGTCCATTTTTTTGGTGTCTATGATGGGCATGGAGGCTTTCAGGTTGCTAATTACTGTCAAGAACGTCTACACTCAGCGTTGATTGAGGAGATTGAAGCTCGGCAGTCAAGTTTGGATGAAGCAAATGGAGAAACAATTGGCAGGAAAATTGGAATAAAGTATTGTTCAATTAAAGTAGATGATGAGATTGAAACTATTGCTCCTGAGACTGCTGGCTCCACTGCAGTGGTTGCCATTTTAAATCAAACCCACATAATTGTTGCAAATTGTGGGGATTCGAGGGCTGTCTTGTATCGTGGGAAAGAAGCCATAGCGTTGTCTTCTGACCACAAACCGAATCGAGAGGATGAGAGGGCAAGAATCGAAGCAGCAGGAGGAAGAGTCATACATTGGAAAGGATACAGAGTTCTTGGTGTCTTGGCCATGTCAAGGTCCATAGGTGATAGATACTTGAAACCATGGATAATTCCAGAACCAGAAGTCAATATTGTGCAAAGAGAGAAAAATGATGAGTGCCTTATTTTAGCAAGTGATGGTTATGGGATGTAA
Protein sequence

>Msa0662540

MGFSSICGNRPEMEDAVAVKPQLFQVPSQMLMDDHLNENTKYPQVHFFGVYDGHGGFQVANYCQERLHSALIEEIEARQSSLDEANGETIGRKIGIKYCSIKVDDEIETIAPETAGSTAVVAILNQTHIIVANCGDSRAVLYRGKEAIALSSDHKPNREDERARIEAAGGRVIHWKGYRVLGVLAMSRSIGDRYLKPWIIPEPEVNIVQREKNDECLILASDGYGM*