AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0507020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGCCAATCGACGAATTGC+AGG 0.267346 3_4:+53911857 None:intergenic
GGAAGAACCCTCTATGGATT+TGG 0.370047 3_4:-53911671 Msa0507020:three_prime_UTR
AGACAAGGAAGAACCCTCTA+TGG 0.396310 3_4:-53911677 Msa0507020:three_prime_UTR
AGGTGTCTTGAAGATCTTCT+TGG 0.405705 3_4:-53911786 Msa0507020:three_prime_UTR
GGGAGGTAAGGGACTTGGAA+AGG 0.449159 3_4:-53911936 Msa0507020:CDS
GGAGGTAAGGGACTTGGAAA+GGG 0.467307 3_4:-53911935 Msa0507020:CDS
GGAAAGGGAGGTAAGGGACT+TGG 0.470633 3_4:-53911941 Msa0507020:CDS
TTCAACCTCCAAATCCATAG+AGG 0.476286 3_4:+53911663 None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG 0.487154 3_4:-53911962 None:intergenic
CAATCGACGAATTGCAGGCT+TGG 0.491738 3_4:+53911862 None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG 0.492819 3_4:-53911948 Msa0507020:CDS
AGTTCTCCGCGATAACATCC+AGG 0.504812 3_4:-53911891 Msa0507020:CDS
AGAACCCTCTATGGATTTGG+AGG 0.508817 3_4:-53911668 Msa0507020:three_prime_UTR
GAAGATCTTCAAGACACCTC+TGG 0.510902 3_4:+53911790 None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG 0.511374 3_4:-53911861 Msa0507020:CDS
TTACATACACTGAGCATGCT+AGG 0.520723 3_4:-53911742 Msa0507020:three_prime_UTR
GAGGAAGACGGTGACAGCCA+TGG 0.546349 3_4:-53911720 Msa0507020:three_prime_UTR
AGGGAGGTGCAAAGAGGCAT+AGG 0.547891 3_4:-53911916 Msa0507020:CDS
AAAATGTCAGGTCGTGGAAA+GGG 0.548959 3_4:-53911956 None:intergenic
GGTAAGGGACTTGGAAAGGG+AGG 0.560743 3_4:-53911932 Msa0507020:CDS
GGTCGTGGAAAGGGAGGTAA+GGG 0.564245 3_4:-53911947 Msa0507020:CDS
GTCTATGCTTTGAAGAGACA+AGG 0.570048 3_4:-53911692 Msa0507020:three_prime_UTR
GAAAATGTCAGGTCGTGGAA+AGG 0.584382 3_4:-53911957 None:intergenic
CGTCGATTGGCGAGAAGAGG+CGG 0.593362 3_4:-53911848 Msa0507020:CDS
GGCGGTGTTAAGAGAATCAG+TGG 0.595979 3_4:-53911830 Msa0507020:CDS
ATTCGTCGATTGGCGAGAAG+AGG 0.604517 3_4:-53911851 Msa0507020:CDS
TTGCAGGCTTGGTGATACCC+TGG 0.613296 3_4:+53911873 None:intergenic
CAAAGCATAGACAACATCCA+TGG 0.629842 3_4:+53911703 None:intergenic
TCAACCTCCAAATCCATAGA+GGG 0.633476 3_4:+53911664 None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG 0.643397 3_4:-53911953 None:intergenic
TGATACCCTGGATGTTATCG+CGG 0.665173 3_4:+53911885 None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG 0.671956 3_4:-53911732 Msa0507020:three_prime_UTR
GTTCTCCGCGATAACATCCA+GGG 0.672360 3_4:-53911890 Msa0507020:CDS
ATCTTAAAGAACAATTACAC+AGG 0.681255 3_4:+53911597 None:intergenic
TTGGAAAGGGAGGTGCAAAG+AGG 0.683014 3_4:-53911922 Msa0507020:CDS
TTGATCTATGAAGAAACCAG+AGG 0.704889 3_4:-53911806 Msa0507020:exon
CATACACTGAGCATGCTAGG+AGG 0.707859 3_4:-53911739 Msa0507020:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCTTAAAGAACAATTACAC+AGG + chr3_4:53911932-53911951 None:intergenic 25.0%
TTGATCTATGAAGAAACCAG+AGG - chr3_4:53911720-53911739 Msa0507020:three_prime_UTR 35.0%
AGGTGTCTTGAAGATCTTCT+TGG - chr3_4:53911740-53911759 Msa0507020:three_prime_UTR 40.0%
TTACATACACTGAGCATGCT+AGG - chr3_4:53911784-53911803 Msa0507020:three_prime_UTR 40.0%
CAAAGCATAGACAACATCCA+TGG + chr3_4:53911826-53911845 None:intergenic 40.0%
!! GTCTATGCTTTGAAGAGACA+AGG - chr3_4:53911834-53911853 Msa0507020:CDS 40.0%
TCAACCTCCAAATCCATAGA+GGG + chr3_4:53911865-53911884 None:intergenic 40.0%
TTCAACCTCCAAATCCATAG+AGG + chr3_4:53911866-53911885 None:intergenic 40.0%
TGATACCCTGGATGTTATCG+CGG + chr3_4:53911644-53911663 None:intergenic 45.0%
GAAGATCTTCAAGACACCTC+TGG + chr3_4:53911739-53911758 None:intergenic 45.0%
AGACAAGGAAGAACCCTCTA+TGG - chr3_4:53911849-53911868 Msa0507020:CDS 45.0%
GGAAGAACCCTCTATGGATT+TGG - chr3_4:53911855-53911874 Msa0507020:CDS 45.0%
AGAACCCTCTATGGATTTGG+AGG - chr3_4:53911858-53911877 Msa0507020:CDS 45.0%
GGAGGTAAGGGACTTGGAAA+GGG - chr3_4:53911591-53911610 Msa0507020:three_prime_UTR 50.0%
! TTGGAAAGGGAGGTGCAAAG+AGG - chr3_4:53911604-53911623 Msa0507020:three_prime_UTR 50.0%
AGTTCTCCGCGATAACATCC+AGG - chr3_4:53911635-53911654 Msa0507020:intron 50.0%
GTTCTCCGCGATAACATCCA+GGG - chr3_4:53911636-53911655 Msa0507020:intron 50.0%
CAATCGACGAATTGCAGGCT+TGG + chr3_4:53911667-53911686 None:intergenic 50.0%
CAAGCCTGCAATTCGTCGAT+TGG - chr3_4:53911665-53911684 Msa0507020:three_prime_UTR 50.0%
! ATTCGTCGATTGGCGAGAAG+AGG - chr3_4:53911675-53911694 Msa0507020:three_prime_UTR 50.0%
! GGCGGTGTTAAGAGAATCAG+TGG - chr3_4:53911696-53911715 Msa0507020:three_prime_UTR 50.0%
CATACACTGAGCATGCTAGG+AGG - chr3_4:53911787-53911806 Msa0507020:three_prime_UTR 50.0%
TGAGCATGCTAGGAGGAAGA+CGG - chr3_4:53911794-53911813 Msa0507020:three_prime_UTR 50.0%
AGGTCGTGGAAAGGGAGGTA+AGG - chr3_4:53911578-53911597 Msa0507020:three_prime_UTR 55.0%
GGTCGTGGAAAGGGAGGTAA+GGG - chr3_4:53911579-53911598 Msa0507020:three_prime_UTR 55.0%
GGAAAGGGAGGTAAGGGACT+TGG - chr3_4:53911585-53911604 Msa0507020:three_prime_UTR 55.0%
GGGAGGTAAGGGACTTGGAA+AGG - chr3_4:53911590-53911609 Msa0507020:three_prime_UTR 55.0%
GGTAAGGGACTTGGAAAGGG+AGG - chr3_4:53911594-53911613 Msa0507020:three_prime_UTR 55.0%
! AGGGAGGTGCAAAGAGGCAT+AGG - chr3_4:53911610-53911629 Msa0507020:three_prime_UTR 55.0%
!! TTGCAGGCTTGGTGATACCC+TGG + chr3_4:53911656-53911675 None:intergenic 55.0%
CTCGCCAATCGACGAATTGC+AGG + chr3_4:53911672-53911691 None:intergenic 55.0%
! CGTCGATTGGCGAGAAGAGG+CGG - chr3_4:53911678-53911697 Msa0507020:three_prime_UTR 60.0%
GAGGAAGACGGTGACAGCCA+TGG - chr3_4:53911806-53911825 Msa0507020:exon 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 53911577 53911971 53911577 ID=Msa0507020;Name=Msa0507020
chr3_4 mRNA 53911577 53911971 53911577 ID=Msa0507020-mRNA-1;Parent=Msa0507020;Name=Msa0507020-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|0|0|2|227|48
chr3_4 exon 53911577 53911640 53911577 ID=Msa0507020-mRNA-1:exon:11977;Parent=Msa0507020-mRNA-1
chr3_4 exon 53911662 53911971 53911662 ID=Msa0507020-mRNA-1:exon:11976;Parent=Msa0507020-mRNA-1
chr3_4 CDS 53911825 53911971 53911825 ID=Msa0507020-mRNA-1:cds;Parent=Msa0507020-mRNA-1
chr3_4 three_prime_UTR 53911662 53911824 53911662 ID=Msa0507020-mRNA-1:three_prime_utr;Parent=Msa0507020-mRNA-1
chr3_4 three_prime_UTR 53911577 53911640 53911577 ID=Msa0507020-mRNA-1:three_prime_utr;Parent=Msa0507020-mRNA-1
Gene Sequence

>Msa0507020

CAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTCCGCGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGCGGTGTTAAGAGAATCAGTGGATTGA

Protein sequence

>Msa0507020

QVVEREVRDLEREVQRGIGKFSAITSRVSPSLQFVDWREEAVLRESVD*