AlfalfaGEDB | Msa1034150

M. sativa cultivar zhongmu-4 / Msa1034150

KEGG: K03553|K04482|K10871
GO: GO:0000003 GO:0000018 GO:0000150 GO:0000217 GO:0000228 GO:0000280 GO:0000400 GO:0000707 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000730 GO:0000781 GO:0000784 GO:0002831 GO:0002833 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008092 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010520 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0016444 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032101 GO:0032103 GO:0032200 GO:0032991 GO:0033043 GO:0033061 GO:0033063 GO:0033554 GO:0034622 GO:0034641 GO:0035825 GO:0036297 GO:0040020 GO:0042148 GO:0042592 GO:0042623 GO:0043015 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0043902 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045003 GO:0046483 GO:0048285 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051276 GO:0051321 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060249 GO:0060255 GO:0060631 GO:0061982 GO:0065003 GO:0065004 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090735 GO:0097159 GO:0097196 GO:0098687 GO:0140013 GO:0140097 GO:1900424 GO:1900426 GO:1901360 GO:1901363 GO:1903046 GO:1990391 GO:2000241
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034150	AES81312.1	92.308	312	22	1	1	312	1	310	0.0	522

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034150	G7KSL2	92.308	312	22	1	1	312	1	310	0.0	522

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001790	Msa1034150	0.810719	1.002315e-50	-8.615850e-47
Msa0003890	Msa1034150	0.828874	7.053406e-55	-8.615850e-47
Msa0004840	Msa1034150	0.823421	1.397894e-53	-8.615850e-47
Msa0005270	Msa1034150	0.817204	3.721763e-52	-8.615850e-47
Msa0006110	Msa1034150	0.807346	5.287074e-50	-8.615850e-47
Msa0007440	Msa1034150	0.844601	6.835295e-59	-8.615850e-47
Msa0009430	Msa1034150	0.817857	2.652474e-52	-8.615850e-47
Msa0011170	Msa1034150	0.839369	1.651289e-57	-8.615850e-47
Msa0011960	Msa1034150	0.802157	6.409317e-49	-8.615850e-47
Msa0015090	Msa1034150	0.847233	1.316475e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034150	MtrunA17_Chr7g0257031	92.949	312	20	1	1	312	1	310	0.0	514
Msa1034150	MtrunA17_Chr7g0256981	77.848	316	64	4	1	312	1	314	4.26e-156	439
Msa1034150	MtrunA17_Chr7g0257011	63.782	312	87	7	1	312	1	286	1.57e-123	355
Msa1034150	MtrunA17_Chr7g0256971	72.000	250	63	3	35	282	3	247	1.58e-118	342
Msa1034150	MtrunA17_Chr1g0172581	44.910	167	81	5	65	231	1	156	3.20e-35	125
Msa1034150	MtrunA17_Chr3g0085991	31.780	236	126	6	26	247	24	238	7.23e-31	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034150	AT5G03990.1	40.838	191	90	3	1	171	1	188	1.99e-40	143
Msa1034150	AT3G51940.1	39.286	112	67	1	26	136	20	131	8.03e-24	101
Msa1034150	AT3G51940.2	42.424	99	56	1	39	136	37	135	2.65e-23	99.8
Msa1034150	AT5G03990.2	48.352	91	44	1	65	155	1	88	2.06e-20	88.6

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Find 183 sgRNAs with CRISPR-Local

Find 590 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATGAAACAAACTCT+TGG	0.438543	7_1:-69539420	Msa1034150:CDS
AAAATTCTAGACACTTTAAG+AGG	0.406500	7_1:-69534184	Msa1034150:three_prime_UTR
AAACCTATGAAGCTGCTAGA+GGG	0.620303	7_1:-69539028	Msa1034150:three_prime_UTR
AAACCTGCTTATCTATTTGT+AGG	0.277089	7_1:-69533083	Msa1034150:three_prime_UTR
AAAGAATATGGATGGCAAAA+TGG	0.329913	7_1:-69539364	Msa1034150:CDS
AAAGACTGAACTGGCCAAAT+CGG	0.478619	7_1:-69533470	Msa1034150:three_prime_UTR
AAATAATGAAACAAACTCTT+GGG	0.477297	7_1:-69539419	Msa1034150:CDS
AAATAGTGGGAGAGGAAATT+GGG	0.332117	7_1:-69539269	Msa1034150:CDS
AAATCGGTATCAAGTTTGGA+AGG	0.627405	7_1:-69533454	Msa1034150:three_prime_UTR
AAATGATGACTCAATCAGTA+TGG	0.427788	7_1:-69534037	Msa1034150:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATTGGAGAAGAAACCAA+AGG	-	chr7_1:69533080-69533099	Msa1034150:three_prime_UTR	30.0%
TTGTTGCATAGTTGAAAAAG+TGG	-	chr7_1:69533255-69533274	Msa1034150:three_prime_UTR	30.0%
TGTTGCATAGTTGAAAAAGT+GGG	-	chr7_1:69533256-69533275	Msa1034150:three_prime_UTR	30.0%
AAGTAAAGTGATCACAATCA+AGG	+	chr7_1:69533377-69533396	None:intergenic	30.0%
TAGTTATCTATCAACCCTTA+TGG	+	chr7_1:69533448-69533467	None:intergenic	30.0%
TATTACAGAACATAATCAGC+AGG	+	chr7_1:69533493-69533512	None:intergenic	30.0%
AATAAACATCTCCTTGAACA+TGG	+	chr7_1:69533725-69533744	None:intergenic	30.0%
ATGTTCAAGGAGATGTTTAT+TGG	-	chr7_1:69533724-69533743	Msa1034150:intron	30.0%
TTCATTGAATAGCCAAATCT+AGG	+	chr7_1:69533894-69533913	None:intergenic	30.0%
ACAATGCAAAAAACAGGTAT+TGG	-	chr7_1:69534214-69534233	Msa1034150:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	69532905	69541081	69532905	ID=Msa1034150;Name=Msa1034150
chr7_1	mRNA	69532905	69541081	69532905	ID=Msa1034150-mRNA-1;Parent=Msa1034150;Name=Msa1034150-mRNA-1;_AED=0.38;_eAED=0.38;_QI=171\|1\|1\|1\|0.4\|0.33\|6\|1779\|312
chr7_1	exon	69532905	69533554	69532905	ID=Msa1034150-mRNA-1:exon:16382;Parent=Msa1034150-mRNA-1
chr7_1	exon	69533755	69534525	69533755	ID=Msa1034150-mRNA-1:exon:16381;Parent=Msa1034150-mRNA-1
chr7_1	exon	69536765	69536851	69536765	ID=Msa1034150-mRNA-1:exon:16380;Parent=Msa1034150-mRNA-1
chr7_1	exon	69538095	69538266	69538095	ID=Msa1034150-mRNA-1:exon:16379;Parent=Msa1034150-mRNA-1
chr7_1	exon	69538985	69539925	69538985	ID=Msa1034150-mRNA-1:exon:16378;Parent=Msa1034150-mRNA-1
chr7_1	exon	69540814	69541081	69540814	ID=Msa1034150-mRNA-1:exon:16377;Parent=Msa1034150-mRNA-1
chr7_1	five_prime_UTR	69540911	69541081	69540911	ID=Msa1034150-mRNA-1:five_prime_utr;Parent=Msa1034150-mRNA-1
chr7_1	CDS	69540814	69540910	69540814	ID=Msa1034150-mRNA-1:cds;Parent=Msa1034150-mRNA-1
chr7_1	CDS	69539084	69539925	69539084	ID=Msa1034150-mRNA-1:cds;Parent=Msa1034150-mRNA-1
chr7_1	three_prime_UTR	69538985	69539083	69538985	ID=Msa1034150-mRNA-1:three_prime_utr;Parent=Msa1034150-mRNA-1
chr7_1	three_prime_UTR	69538095	69538266	69538095	ID=Msa1034150-mRNA-1:three_prime_utr;Parent=Msa1034150-mRNA-1
chr7_1	three_prime_UTR	69536765	69536851	69536765	ID=Msa1034150-mRNA-1:three_prime_utr;Parent=Msa1034150-mRNA-1
chr7_1	three_prime_UTR	69533755	69534525	69533755	ID=Msa1034150-mRNA-1:three_prime_utr;Parent=Msa1034150-mRNA-1
chr7_1	three_prime_UTR	69532905	69533554	69532905	ID=Msa1034150-mRNA-1:three_prime_utr;Parent=Msa1034150-mRNA-1

Gene Sequence

>Msa1034150

ATGGATAATTGGAGAAGAAACCAAAGGATCAGTAACCATCATCAAGTGGGTCAGTGGGGATCCTCCTTCTCATCCTATAACGGAAAACCACCTTTAGATAATAGGTTTCCAACAGTGCCTTTGTGGGAAAAGCAGTTTTGTGCTTCGGTGGGCTCAGTTCCATGGCGAAAGCTAATAGAAGACAAGAGGTACATGTATATGCATCCCAAAGTGGTAAACTGGGACGACTCTGCTGTCAAAGAGGCATTCGACAATGCAAAAAACAGGTATTGGGCTGAGATCAACAACATTCCCTGGGACATACCATTGCCTGATCCTAACATTTACATAGATGATGTAGATTCGAATGCAAGTGTTGATTCTGAACTTTATTTGGATGTGGAAATAGAAGCAGAGGCCCGCCCCAACATAGAAGAAAAAGGAGAGGCGGCTGTGACTCTAGGAAGTTCTCTCCTCTTGAATCAGTCCTCTTCTGGTCTTGGATTGGGACCTACTGGATGGGGGGACGAAGAAGACGAAAAGTTAACAAAGCCTTCTGAACCAAATTATGCTGCTCTGGGATGGGAATCAAACCAACATGAAAATAATGAAACAAACTCTTGGGAACAGTATCATGCTCCCGTTGAACATGCTGCAAAAGAATATGGATGGCAAAATGGTCAGAATGATTCTCAGGGATGGAATCAGAGGGAGCGATATGGTGGTGACTTGTATAACAAGTATCAAGGAAGAAATAGTGGGAGAGGAAATTGGGGAACATGGGATGGATTTAACAGAAAGAAAGAAAACAACATGTCTTGGTCTAAGAACCATGGATATCATCATGGCACTAATGAGTATCAGATGAATAGAGGAGGAAGAAGAAATGGAGGAAGAGGAGGAGGTGGGGGAAGGAGGGGAAAATTTGCTTATAAGGTTGCTACTCCTAGTGCATGGTAA

Protein sequence

>Msa1034150

MDNWRRNQRISNHHQVGQWGSSFSSYNGKPPLDNRFPTVPLWEKQFCASVGSVPWRKLIEDKRYMYMHPKVVNWDDSAVKEAFDNAKNRYWAEINNIPWDIPLPDPNIYIDDVDSNASVDSELYLDVEIEAEARPNIEEKGEAAVTLGSSLLLNQSSSGLGLGPTGWGDEEDEKLTKPSEPNYAALGWESNQHENNETNSWEQYHAPVEHAAKEYGWQNGQNDSQGWNQRERYGGDLYNKYQGRNSGRGNWGTWDGFNRKKENNMSWSKNHGYHHGTNEYQMNRGGRRNGGRGGGGGRRGKFAYKVATPSAW*