AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0974630


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0974630 MtrunA17_Chr6g0453281 98.312 237 4 0 1 237 1 237 5.33e-174 478
Msa0974630 MtrunA17_Chr7g0217561 61.603 237 71 3 1 237 1 217 3.74e-87 257
Msa0974630 MtrunA17_Chr2g0287451 39.516 124 62 4 114 234 79 192 2.35e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0974630 AT3G01170.1 55.000 240 80 5 1 237 1 215 4.89e-83 247
Msa0974630 AT5G15260.2 55.691 246 88 9 1 237 1 234 2.06e-74 226
Msa0974630 AT5G15260.1 55.691 246 88 9 1 237 1 234 2.06e-74 226
Msa0974630 AT3G06180.1 40.000 165 94 3 75 237 66 227 5.97e-23 93.6
Msa0974630 AT5G19025.1 51.515 99 45 2 139 237 150 245 1.55e-22 93.2
Msa0974630 AT5G19025.2 50.526 95 45 1 139 233 150 242 3.33e-22 92.4
Msa0974630 AT3G01170.2 43.220 118 48 3 1 116 1 101 2.22e-21 87.4

Find 45 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTATATTTATATATCTAT+TGG 0.238022 6_4:-7821728 Msa0974630:CDS
GTCATTTCAGATTATGAGTA+TGG 0.244984 6_4:-7821786 Msa0974630:CDS
TAACAGAATTTGATAAACTA+TGG 0.289659 6_4:+7821924 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.289963 6_4:-7821411 Msa0974630:CDS
AAGAAGATGGCACCGATTAA+TGG 0.325801 6_4:-7821473 Msa0974630:CDS
CCAATAGATATATAAATATA+AGG 0.341548 6_4:+7821728 None:intergenic
ATGGCGAATTCGATCGAATT+CGG 0.378268 6_4:-7822001 Msa0974630:CDS
TTTGTGCTGCATTGGCTACA+TGG 0.387252 6_4:-7821694 Msa0974630:CDS
TTCTTCAAACCCTTACAATT+CGG 0.389737 6_4:+7821635 None:intergenic
CGAAGTTTCTTCGTCGGTCC+CGG 0.401109 6_4:+7821392 None:intergenic
GTTGGTAGGTTAGAAGTTCC+GGG 0.404512 6_4:-7821410 Msa0974630:CDS
ATCACTAAAAGTTCAACATA+AGG 0.412821 6_4:+7821812 None:intergenic
CTAATCAATTTCAAAGGAAT+AGG 0.420647 6_4:-7821952 Msa0974630:CDS
ATGTGGTAATCCGAATTGTA+AGG 0.420695 6_4:-7821645 Msa0974630:CDS
GATGGCGGTGTTTGTAAGAA+GGG 0.429289 6_4:-7821545 Msa0974630:CDS
AGCACAGCTCTTCCATTAAT+CGG 0.433317 6_4:+7821461 None:intergenic
ATTGCTGTTGTTATTGCTTG+TGG 0.436635 6_4:-7821848 Msa0974630:CDS
AGATGGCGGTGTTTGTAAGA+AGG 0.445969 6_4:-7821546 Msa0974630:CDS
CTGTGCTTGTGCTGAGAGCT+AGG 0.461752 6_4:-7821445 Msa0974630:CDS
GGTGTGGTTGTTCTGTTGGT+AGG 0.475981 6_4:-7821424 Msa0974630:CDS
GCTAGGTGTGGTTGTTCTGT+TGG 0.477365 6_4:-7821428 Msa0974630:CDS
TTCGCCATGGATGATTGAAC+TGG 0.483771 6_4:+7822016 None:intergenic
ATCTTCCGAAGTTTCTTCGT+CGG 0.488611 6_4:+7821386 None:intergenic
TCACTAAAAGTTCAACATAA+GGG 0.493997 6_4:+7821813 None:intergenic
TTGTGCTGCATTGGCTACAT+GGG 0.507240 6_4:-7821693 Msa0974630:CDS
ATCAATTTCAAAGGAATAGG+AGG 0.510788 6_4:-7821949 Msa0974630:CDS
AAGTGTTGTTTGTGCTGCAT+TGG 0.523765 6_4:-7821702 Msa0974630:CDS
ACTTCGGAAGATCAAGAAGT+AGG 0.555452 6_4:-7821375 None:intergenic
AGCAAACTAATCAATTTCAA+AGG 0.564372 6_4:-7821958 Msa0974630:CDS
TGTGGTAATCCGAATTGTAA+GGG 0.569597 6_4:-7821644 Msa0974630:CDS
TGAAGCGGAGCTTAAGAAGA+TGG 0.571103 6_4:-7821486 Msa0974630:CDS
TACAAAGAGAGATTGAGCTA+TGG 0.571615 6_4:+7822056 None:intergenic
ATTGCCGCGTGATCATCATA+AGG 0.580739 6_4:-7821513 Msa0974630:CDS
ACCTCCAGTTCAATCATCCA+TGG 0.585489 6_4:-7822020 Msa0974630:CDS
CCGCCATCTTTCACAAAAGA+CGG 0.590770 6_4:+7821560 None:intergenic
GTTTGCACAAGTAACAAATG+TGG 0.622560 6_4:-7821662 Msa0974630:CDS
TCATCATAAGGAACTTGAAG+CGG 0.636755 6_4:-7821501 Msa0974630:CDS
CGGGACCGACGAAGAAACTT+CGG 0.636873 6_4:-7821391 Msa0974630:CDS
GCCATGGATGATTGAACTGG+AGG 0.647249 6_4:+7822019 None:intergenic
GAATTCGATCGAATTCGCCA+TGG 0.649026 6_4:+7822003 None:intergenic
TGTGCTGCATTGGCTACATG+GGG 0.654737 6_4:-7821692 Msa0974630:CDS
CTTGTGCTGAGAGCTAGGTG+TGG 0.697858 6_4:-7821440 Msa0974630:CDS
GCTGATCTAGATCGATCACA+AGG 0.698983 6_4:+7821893 None:intergenic
GATCTAGATCGATCACAAGG+TGG 0.717259 6_4:+7821896 None:intergenic
AGTTCCTTATGATGATCACG+CGG 0.724998 6_4:+7821509 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAATAGATATATAAATATA+AGG + chr6_4:7821718-7821737 None:intergenic 15.0%
!! CCTTATATTTATATATCTAT+TGG - chr6_4:7821715-7821734 Msa0974630:CDS 15.0%
!! TAACAGAATTTGATAAACTA+TGG + chr6_4:7821522-7821541 None:intergenic 20.0%
! AGCAAACTAATCAATTTCAA+AGG - chr6_4:7821485-7821504 Msa0974630:CDS 25.0%
! CTAATCAATTTCAAAGGAAT+AGG - chr6_4:7821491-7821510 Msa0974630:CDS 25.0%
! TCACTAAAAGTTCAACATAA+GGG + chr6_4:7821633-7821652 None:intergenic 25.0%
! ATCACTAAAAGTTCAACATA+AGG + chr6_4:7821634-7821653 None:intergenic 25.0%
!!! TATGGTGTGTTTTTTGATTA+TGG - chr6_4:7821675-7821694 Msa0974630:CDS 25.0%
ATCAATTTCAAAGGAATAGG+AGG - chr6_4:7821494-7821513 Msa0974630:CDS 30.0%
GTCATTTCAGATTATGAGTA+TGG - chr6_4:7821657-7821676 Msa0974630:CDS 30.0%
TTCTTCAAACCCTTACAATT+CGG + chr6_4:7821811-7821830 None:intergenic 30.0%
TACAAAGAGAGATTGAGCTA+TGG + chr6_4:7821390-7821409 None:intergenic 35.0%
! ATTGCTGTTGTTATTGCTTG+TGG - chr6_4:7821595-7821614 Msa0974630:CDS 35.0%
GTTTGCACAAGTAACAAATG+TGG - chr6_4:7821781-7821800 Msa0974630:CDS 35.0%
ATGTGGTAATCCGAATTGTA+AGG - chr6_4:7821798-7821817 Msa0974630:CDS 35.0%
TGTGGTAATCCGAATTGTAA+GGG - chr6_4:7821799-7821818 Msa0974630:CDS 35.0%
TCATCATAAGGAACTTGAAG+CGG - chr6_4:7821942-7821961 Msa0974630:CDS 35.0%
ATGGCGAATTCGATCGAATT+CGG - chr6_4:7821442-7821461 Msa0974630:CDS 40.0%
!! AAGTGTTGTTTGTGCTGCAT+TGG - chr6_4:7821741-7821760 Msa0974630:CDS 40.0%
! TCACCGTCTTTTGTGAAAGA+TGG - chr6_4:7821880-7821899 Msa0974630:CDS 40.0%
AGTTCCTTATGATGATCACG+CGG + chr6_4:7821937-7821956 None:intergenic 40.0%
!! AAGAAGATGGCACCGATTAA+TGG - chr6_4:7821970-7821989 Msa0974630:CDS 40.0%
AGCACAGCTCTTCCATTAAT+CGG + chr6_4:7821985-7822004 None:intergenic 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG - chr6_4:7822032-7822051 Msa0974630:CDS 40.0%
ATCTTCCGAAGTTTCTTCGT+CGG + chr6_4:7822060-7822079 None:intergenic 40.0%
ACCTCCAGTTCAATCATCCA+TGG - chr6_4:7821423-7821442 Msa0974630:CDS 45.0%
TTCGCCATGGATGATTGAAC+TGG + chr6_4:7821430-7821449 None:intergenic 45.0%
GAATTCGATCGAATTCGCCA+TGG + chr6_4:7821443-7821462 None:intergenic 45.0%
GATCTAGATCGATCACAAGG+TGG + chr6_4:7821550-7821569 None:intergenic 45.0%
GCTGATCTAGATCGATCACA+AGG + chr6_4:7821553-7821572 None:intergenic 45.0%
!! TTTGTGCTGCATTGGCTACA+TGG - chr6_4:7821749-7821768 Msa0974630:CDS 45.0%
!! TTGTGCTGCATTGGCTACAT+GGG - chr6_4:7821750-7821769 Msa0974630:CDS 45.0%
CCGCCATCTTTCACAAAAGA+CGG + chr6_4:7821886-7821905 None:intergenic 45.0%
! CCGTCTTTTGTGAAAGATGG+CGG - chr6_4:7821883-7821902 Msa0974630:CDS 45.0%
! AGATGGCGGTGTTTGTAAGA+AGG - chr6_4:7821897-7821916 Msa0974630:CDS 45.0%
! GATGGCGGTGTTTGTAAGAA+GGG - chr6_4:7821898-7821917 Msa0974630:CDS 45.0%
ATTGCCGCGTGATCATCATA+AGG - chr6_4:7821930-7821949 Msa0974630:CDS 45.0%
TGAAGCGGAGCTTAAGAAGA+TGG - chr6_4:7821957-7821976 Msa0974630:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG - chr6_4:7822033-7822052 Msa0974630:CDS 45.0%
GCCATGGATGATTGAACTGG+AGG + chr6_4:7821427-7821446 None:intergenic 50.0%
!! TGTGCTGCATTGGCTACATG+GGG - chr6_4:7821751-7821770 Msa0974630:CDS 50.0%
GCTAGGTGTGGTTGTTCTGT+TGG - chr6_4:7822015-7822034 Msa0974630:CDS 50.0%
GGTGTGGTTGTTCTGTTGGT+AGG - chr6_4:7822019-7822038 Msa0974630:CDS 50.0%
CTGTGCTTGTGCTGAGAGCT+AGG - chr6_4:7821998-7822017 Msa0974630:CDS 55.0%
CTTGTGCTGAGAGCTAGGTG+TGG - chr6_4:7822003-7822022 Msa0974630:CDS 55.0%
CGAAGTTTCTTCGTCGGTCC+CGG + chr6_4:7822054-7822073 None:intergenic 55.0%
CGGGACCGACGAAGAAACTT+CGG - chr6_4:7822052-7822071 Msa0974630:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 7821376 7822089 7821376 ID=Msa0974630;Name=Msa0974630
chr6_4 mRNA 7821376 7822089 7821376 ID=Msa0974630-mRNA-1;Parent=Msa0974630;Name=Msa0974630-mRNA-1;_AED=0.16;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|237
chr6_4 exon 7821376 7822089 7821376 ID=Msa0974630-mRNA-1:exon:2961;Parent=Msa0974630-mRNA-1
chr6_4 CDS 7821376 7822089 7821376 ID=Msa0974630-mRNA-1:cds;Parent=Msa0974630-mRNA-1
Gene Sequence

>Msa0974630

ATGGTCTATTTCCATAGCTCAATCTCTCTTTGTAAGTCAGTTGATCAACCTCCAGTTCAATCATCCATGGCGAATTCGATCGAATTCGGATCGAAATGTTCGAAATCTAAGCAAACTAATCAATTTCAAAGGAATAGGAGGAACCATAGTTTATCAAATTCTGTTAAGATTCCACCTTGTGATCGATCTAGATCAGCTATGATTGATGTTGTTATGTTCATTGCTGTTGTTATTGCTTGTGGATTTTTGTTTTTCCCTTATGTTGAACTTTTAGTGATTAAGTCATTTCAGATTATGAGTATGGTGTGTTTTTTGATTATGGAAGAAGTTTCTTATGCACCTTATATTTATATATCTATTGGAATAAGTGTTGTTTGTGCTGCATTGGCTACATGGGGTGTTATTGTTTGCACAAGTAACAAATGTGGTAATCCGAATTGTAAGGGTTTGAAGAAAGCTGCAGAATTTGATATTCAGTTAGAGACTGAAGATTGTGTGAAGAATTCACCGTCTTTTGTGAAAGATGGCGGTGTTTGTAAGAAGGGTTTGTTTGAATTGCCGCGTGATCATCATAAGGAACTTGAAGCGGAGCTTAAGAAGATGGCACCGATTAATGGAAGAGCTGTGCTTGTGCTGAGAGCTAGGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGACCGACGAAGAAACTTCGGAAGATCAAGAAGTAG

Protein sequence

>Msa0974630

MVYFHSSISLCKSVDQPPVQSSMANSIEFGSKCSKSKQTNQFQRNRRNHSLSNSVKIPPCDRSRSAMIDVVMFIAVVIACGFLFFPYVELLVIKSFQIMSMVCFLIMEEVSYAPYIYISIGISVVCAALATWGVIVCTSNKCGNPNCKGLKKAAEFDIQLETEDCVKNSPSFVKDGGVCKKGLFELPRDHHKELEAELKKMAPINGRAVLVLRARCGCSVGRLEVPGPTKKLRKIKK*