AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0727040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727040 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.19e-38 124
Msa0727040 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.19e-38 124
Msa0727040 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.85e-37 120
Msa0727040 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 8.03e-26 90.9
Msa0727040 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0727040 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0727040 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0727040 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0727040 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 6.36e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727040 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.54e-29 100
Msa0727040 ATCG00630.1 84.375 32 5 0 15 46 1 32 9.35e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 5_1:-28922939 Msa0727040:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 5_1:-28922895 Msa0727040:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 5_1:+28922799 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 5_1:-28923579 Msa0727040:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 5_1:-28922894 Msa0727040:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 5_1:-28923562 Msa0727040:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 5_1:-28922840 Msa0727040:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 5_1:-28923572 Msa0727040:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 5_1:+28922800 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 5_1:+28922830 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 5_1:-28923593 Msa0727040:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 5_1:+28923577 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 5_1:-28922893 Msa0727040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATATAAAATTAAT+TGG - chr5_1:28923327-28923346 Msa0727040:intron 0.0%
!! AAAATTAATATAAAATTAAT+TGG - chr5_1:28923327-28923346 Msa0727040:intron 0.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr5_1:28922940-28922959 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr5_1:28923066-28923085 Msa0727040:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr5_1:28923160-28923179 None:intergenic 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr5_1:28922940-28922959 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr5_1:28923066-28923085 Msa0727040:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr5_1:28923160-28923179 None:intergenic 15.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr5_1:28922787-28922806 Msa0727040:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr5_1:28922939-28922958 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr5_1:28922941-28922960 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr5_1:28922939-28922958 Msa0727040:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr5_1:28922994-28923013 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr5_1:28923069-28923088 Msa0727040:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr5_1:28923075-28923094 Msa0727040:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr5_1:28923191-28923210 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr5_1:28923491-28923510 Msa0727040:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr5_1:28923527-28923546 Msa0727040:intron 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr5_1:28922787-28922806 Msa0727040:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr5_1:28922939-28922958 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr5_1:28922941-28922960 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr5_1:28922939-28922958 Msa0727040:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr5_1:28922994-28923013 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr5_1:28923069-28923088 Msa0727040:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr5_1:28923075-28923094 Msa0727040:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr5_1:28923191-28923210 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr5_1:28923491-28923510 Msa0727040:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr5_1:28923527-28923546 Msa0727040:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr5_1:28923011-28923030 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr5_1:28923195-28923214 Msa0727040:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr5_1:28923196-28923215 Msa0727040:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr5_1:28923445-28923464 Msa0727040:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr5_1:28923011-28923030 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr5_1:28923195-28923214 Msa0727040:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr5_1:28923196-28923215 Msa0727040:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr5_1:28923445-28923464 Msa0727040:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - chr5_1:28922790-28922809 Msa0727040:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr5_1:28922828-28922847 Msa0727040:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr5_1:28922900-28922919 Msa0727040:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr5_1:28922944-28922963 Msa0727040:intron 30.0%
GAAAAAAAAAACGAACGAAG+AGG + chr5_1:28923012-28923031 None:intergenic 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr5_1:28923101-28923120 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr5_1:28923175-28923194 Msa0727040:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr5_1:28923448-28923467 Msa0727040:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr5_1:28923481-28923500 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr5_1:28923535-28923554 Msa0727040:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr5_1:28923536-28923555 Msa0727040:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr5_1:28923537-28923556 Msa0727040:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr5_1:28923633-28923652 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr5_1:28923634-28923653 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - chr5_1:28922790-28922809 Msa0727040:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr5_1:28922828-28922847 Msa0727040:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr5_1:28922900-28922919 Msa0727040:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr5_1:28922944-28922963 Msa0727040:intron 30.0%
GAAAAAAAAAACGAACGAAG+AGG + chr5_1:28923012-28923031 None:intergenic 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr5_1:28923101-28923120 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr5_1:28923175-28923194 Msa0727040:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr5_1:28923448-28923467 Msa0727040:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr5_1:28923481-28923500 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr5_1:28923535-28923554 Msa0727040:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr5_1:28923536-28923555 Msa0727040:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr5_1:28923537-28923556 Msa0727040:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr5_1:28923633-28923652 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr5_1:28923634-28923653 None:intergenic 30.0%
! TTGATTCTAGTTATTGGCAC+GGG - chr5_1:28922945-28922964 Msa0727040:intron 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr5_1:28923104-28923123 Msa0727040:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr5_1:28923124-28923143 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr5_1:28923125-28923144 None:intergenic 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr5_1:28923406-28923425 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr5_1:28923455-28923474 Msa0727040:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr5_1:28923603-28923622 None:intergenic 35.0%
! TTGATTCTAGTTATTGGCAC+GGG - chr5_1:28922945-28922964 Msa0727040:intron 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr5_1:28923104-28923123 Msa0727040:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr5_1:28923124-28923143 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr5_1:28923125-28923144 None:intergenic 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr5_1:28923406-28923425 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr5_1:28923455-28923474 Msa0727040:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr5_1:28923603-28923622 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG - chr5_1:28923091-28923110 Msa0727040:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr5_1:28923113-28923132 Msa0727040:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr5_1:28923590-28923609 Msa0727040:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr5_1:28923091-28923110 Msa0727040:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr5_1:28923113-28923132 Msa0727040:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr5_1:28923590-28923609 Msa0727040:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + chr5_1:28922856-28922875 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr5_1:28922922-28922941 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGGA+AGG - chr5_1:28922949-28922968 Msa0727040:intron 45.0%
TCTAGTTATTGGCACGGGAA+GGG - chr5_1:28922950-28922969 Msa0727040:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + chr5_1:28922856-28922875 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr5_1:28922922-28922941 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGGA+AGG - chr5_1:28922949-28922968 Msa0727040:intron 45.0%
TCTAGTTATTGGCACGGGAA+GGG - chr5_1:28922950-28922969 Msa0727040:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG - chr5_1:28922837-28922856 Msa0727040:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr5_1:28922851-28922870 Msa0727040:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr5_1:28922858-28922877 Msa0727040:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr5_1:28922921-28922940 None:intergenic 50.0%
CTAGTTATTGGCACGGGAAG+GGG - chr5_1:28922951-28922970 Msa0727040:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr5_1:28923462-28923481 Msa0727040:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - chr5_1:28922837-28922856 Msa0727040:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr5_1:28922851-28922870 Msa0727040:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr5_1:28922858-28922877 Msa0727040:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr5_1:28922921-28922940 None:intergenic 50.0%
CTAGTTATTGGCACGGGAAG+GGG - chr5_1:28922951-28922970 Msa0727040:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr5_1:28923462-28923481 Msa0727040:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr5_1:28922868-28922887 Msa0727040:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr5_1:28923464-28923483 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr5_1:28923461-28923480 Msa0727040:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr5_1:28922868-28922887 Msa0727040:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr5_1:28923464-28923483 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr5_1:28923461-28923480 Msa0727040:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 28922778 28923674 28922778 ID=Msa0727040;Name=Msa0727040
chr5_1 mRNA 28922778 28923674 28922778 ID=Msa0727040-mRNA-1;Parent=Msa0727040;Name=Msa0727040-mRNA-1;_AED=0.45;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|105
chr5_1 exon 28923542 28923674 28923542 ID=Msa0727040-mRNA-1:exon:9970;Parent=Msa0727040-mRNA-1
chr5_1 exon 28922778 28922962 28922778 ID=Msa0727040-mRNA-1:exon:9969;Parent=Msa0727040-mRNA-1
chr5_1 CDS 28923542 28923674 28923542 ID=Msa0727040-mRNA-1:cds;Parent=Msa0727040-mRNA-1
chr5_1 CDS 28922778 28922962 28922778 ID=Msa0727040-mRNA-1:cds;Parent=Msa0727040-mRNA-1
Gene Sequence

>Msa0727040

ATGTATTACAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0727040

MYYKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*