AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380540 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa1380540 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa1380540 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa1380540 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa1380540 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1380540 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1380540 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1380540 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1380540 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380540 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa1380540 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 tig0020386:-6065 Msa1380540:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 tig0020386:-6021 Msa1380540:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 tig0020386:+5925 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 tig0020386:-6692 Msa1380540:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 tig0020386:-6020 Msa1380540:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 tig0020386:-6675 Msa1380540:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 tig0020386:-5966 Msa1380540:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 tig0020386:-6685 Msa1380540:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 tig0020386:+5926 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 tig0020386:+5956 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 tig0020386:-6706 Msa1380540:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 tig0020386:+6690 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 tig0020386:-6019 Msa1380540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG + tig0020386:6066-6085 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - tig0020386:6190-6209 Msa1380540:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + tig0020386:6284-6303 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - tig0020386:6440-6459 Msa1380540:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + tig0020386:6066-6085 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - tig0020386:6190-6209 Msa1380540:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + tig0020386:6284-6303 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - tig0020386:6440-6459 Msa1380540:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + tig0020386:5914-5933 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - tig0020386:5913-5932 Msa1380540:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + tig0020386:6065-6084 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + tig0020386:6067-6086 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - tig0020386:6065-6084 Msa1380540:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + tig0020386:6120-6139 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - tig0020386:6193-6212 Msa1380540:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - tig0020386:6199-6218 Msa1380540:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + tig0020386:6315-6334 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - tig0020386:6604-6623 Msa1380540:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - tig0020386:6640-6659 Msa1380540:intron 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + tig0020386:5914-5933 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - tig0020386:5913-5932 Msa1380540:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + tig0020386:6065-6084 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + tig0020386:6067-6086 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - tig0020386:6065-6084 Msa1380540:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + tig0020386:6120-6139 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - tig0020386:6193-6212 Msa1380540:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - tig0020386:6199-6218 Msa1380540:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + tig0020386:6315-6334 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - tig0020386:6604-6623 Msa1380540:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - tig0020386:6640-6659 Msa1380540:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + tig0020386:6137-6156 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + tig0020386:6138-6157 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - tig0020386:6319-6338 Msa1380540:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - tig0020386:6320-6339 Msa1380540:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - tig0020386:6558-6577 Msa1380540:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + tig0020386:6137-6156 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + tig0020386:6138-6157 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - tig0020386:6319-6338 Msa1380540:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - tig0020386:6320-6339 Msa1380540:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - tig0020386:6558-6577 Msa1380540:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - tig0020386:5916-5935 Msa1380540:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - tig0020386:5954-5973 Msa1380540:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - tig0020386:6026-6045 Msa1380540:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - tig0020386:6070-6089 Msa1380540:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + tig0020386:6225-6244 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - tig0020386:6299-6318 Msa1380540:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - tig0020386:6323-6342 Msa1380540:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - tig0020386:6561-6580 Msa1380540:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + tig0020386:6594-6613 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - tig0020386:6648-6667 Msa1380540:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - tig0020386:6649-6668 Msa1380540:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - tig0020386:6650-6669 Msa1380540:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + tig0020386:6746-6765 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + tig0020386:6747-6766 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - tig0020386:5916-5935 Msa1380540:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - tig0020386:5954-5973 Msa1380540:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - tig0020386:6026-6045 Msa1380540:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - tig0020386:6070-6089 Msa1380540:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + tig0020386:6225-6244 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - tig0020386:6299-6318 Msa1380540:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - tig0020386:6323-6342 Msa1380540:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - tig0020386:6561-6580 Msa1380540:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + tig0020386:6594-6613 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - tig0020386:6648-6667 Msa1380540:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - tig0020386:6649-6668 Msa1380540:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - tig0020386:6650-6669 Msa1380540:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + tig0020386:6746-6765 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + tig0020386:6747-6766 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG - tig0020386:6228-6247 Msa1380540:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + tig0020386:6248-6267 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + tig0020386:6249-6268 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - tig0020386:6324-6343 Msa1380540:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + tig0020386:6519-6538 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - tig0020386:6568-6587 Msa1380540:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + tig0020386:6716-6735 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - tig0020386:6228-6247 Msa1380540:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + tig0020386:6248-6267 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + tig0020386:6249-6268 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - tig0020386:6324-6343 Msa1380540:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + tig0020386:6519-6538 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - tig0020386:6568-6587 Msa1380540:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + tig0020386:6716-6735 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG - tig0020386:6215-6234 Msa1380540:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - tig0020386:6237-6256 Msa1380540:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - tig0020386:6703-6722 Msa1380540:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG - tig0020386:6215-6234 Msa1380540:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - tig0020386:6237-6256 Msa1380540:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - tig0020386:6703-6722 Msa1380540:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + tig0020386:5982-6001 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + tig0020386:6048-6067 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - tig0020386:6075-6094 Msa1380540:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - tig0020386:6076-6095 Msa1380540:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + tig0020386:5982-6001 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + tig0020386:6048-6067 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - tig0020386:6075-6094 Msa1380540:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - tig0020386:6076-6095 Msa1380540:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG - tig0020386:5963-5982 Msa1380540:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - tig0020386:5977-5996 Msa1380540:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - tig0020386:5984-6003 Msa1380540:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + tig0020386:6047-6066 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - tig0020386:6077-6096 Msa1380540:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - tig0020386:6575-6594 Msa1380540:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - tig0020386:5963-5982 Msa1380540:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - tig0020386:5977-5996 Msa1380540:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - tig0020386:5984-6003 Msa1380540:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + tig0020386:6047-6066 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - tig0020386:6077-6096 Msa1380540:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - tig0020386:6575-6594 Msa1380540:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - tig0020386:5994-6013 Msa1380540:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + tig0020386:6577-6596 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - tig0020386:6574-6593 Msa1380540:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - tig0020386:5994-6013 Msa1380540:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + tig0020386:6577-6596 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - tig0020386:6574-6593 Msa1380540:intron 55.0%
Chromosome Type Strat End Strand Name
tig0020386 gene 5904 6787 5904 ID=Msa1380540;Name=Msa1380540
tig0020386 mRNA 5904 6787 5904 ID=Msa1380540-mRNA-1;Parent=Msa1380540;Name=Msa1380540-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|105
tig0020386 exon 6655 6787 6655 ID=Msa1380540-mRNA-1:exon:7199;Parent=Msa1380540-mRNA-1
tig0020386 exon 5904 6088 5904 ID=Msa1380540-mRNA-1:exon:7198;Parent=Msa1380540-mRNA-1
tig0020386 CDS 6655 6787 6655 ID=Msa1380540-mRNA-1:cds;Parent=Msa1380540-mRNA-1
tig0020386 CDS 5904 6088 5904 ID=Msa1380540-mRNA-1:cds;Parent=Msa1380540-mRNA-1
Gene Sequence

>Msa1380540

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa1380540

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*