AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983670 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0983670 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0983670 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0983670 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0983670 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0983670 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0983670 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0983670 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0983670 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983670 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0983670 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 6_4:-26787466 Msa0983670:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 6_4:-26787422 Msa0983670:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 6_4:+26787326 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 6_4:-26788093 Msa0983670:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 6_4:-26787421 Msa0983670:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 6_4:-26788076 Msa0983670:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 6_4:-26787367 Msa0983670:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 6_4:-26788086 Msa0983670:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 6_4:+26787327 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 6_4:+26787357 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 6_4:-26788107 Msa0983670:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 6_4:+26788091 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 6_4:-26787420 Msa0983670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG + chr6_4:26787467-26787486 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr6_4:26787591-26787610 Msa0983670:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr6_4:26787685-26787704 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr6_4:26787841-26787860 Msa0983670:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr6_4:26787467-26787486 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr6_4:26787591-26787610 Msa0983670:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr6_4:26787685-26787704 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr6_4:26787841-26787860 Msa0983670:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr6_4:26787315-26787334 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr6_4:26787314-26787333 Msa0983670:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr6_4:26787466-26787485 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr6_4:26787468-26787487 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr6_4:26787466-26787485 Msa0983670:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr6_4:26787521-26787540 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr6_4:26787594-26787613 Msa0983670:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr6_4:26787600-26787619 Msa0983670:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr6_4:26787716-26787735 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr6_4:26788005-26788024 Msa0983670:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr6_4:26788041-26788060 Msa0983670:intron 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr6_4:26787315-26787334 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr6_4:26787314-26787333 Msa0983670:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr6_4:26787466-26787485 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr6_4:26787468-26787487 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr6_4:26787466-26787485 Msa0983670:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr6_4:26787521-26787540 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr6_4:26787594-26787613 Msa0983670:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr6_4:26787600-26787619 Msa0983670:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr6_4:26787716-26787735 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr6_4:26788005-26788024 Msa0983670:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr6_4:26788041-26788060 Msa0983670:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr6_4:26787538-26787557 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr6_4:26787539-26787558 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr6_4:26787720-26787739 Msa0983670:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr6_4:26787721-26787740 Msa0983670:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr6_4:26787959-26787978 Msa0983670:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr6_4:26787538-26787557 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr6_4:26787539-26787558 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr6_4:26787720-26787739 Msa0983670:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr6_4:26787721-26787740 Msa0983670:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr6_4:26787959-26787978 Msa0983670:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - chr6_4:26787317-26787336 Msa0983670:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr6_4:26787355-26787374 Msa0983670:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr6_4:26787427-26787446 Msa0983670:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr6_4:26787471-26787490 Msa0983670:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr6_4:26787626-26787645 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr6_4:26787700-26787719 Msa0983670:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr6_4:26787724-26787743 Msa0983670:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr6_4:26787962-26787981 Msa0983670:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr6_4:26787995-26788014 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr6_4:26788049-26788068 Msa0983670:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr6_4:26788050-26788069 Msa0983670:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr6_4:26788051-26788070 Msa0983670:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr6_4:26788147-26788166 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr6_4:26788148-26788167 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - chr6_4:26787317-26787336 Msa0983670:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr6_4:26787355-26787374 Msa0983670:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr6_4:26787427-26787446 Msa0983670:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr6_4:26787471-26787490 Msa0983670:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr6_4:26787626-26787645 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr6_4:26787700-26787719 Msa0983670:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr6_4:26787724-26787743 Msa0983670:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr6_4:26787962-26787981 Msa0983670:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr6_4:26787995-26788014 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr6_4:26788049-26788068 Msa0983670:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr6_4:26788050-26788069 Msa0983670:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr6_4:26788051-26788070 Msa0983670:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr6_4:26788147-26788166 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr6_4:26788148-26788167 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr6_4:26787629-26787648 Msa0983670:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr6_4:26787649-26787668 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr6_4:26787650-26787669 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr6_4:26787725-26787744 Msa0983670:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr6_4:26787920-26787939 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr6_4:26787969-26787988 Msa0983670:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr6_4:26788117-26788136 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr6_4:26787629-26787648 Msa0983670:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr6_4:26787649-26787668 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr6_4:26787650-26787669 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr6_4:26787725-26787744 Msa0983670:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr6_4:26787920-26787939 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr6_4:26787969-26787988 Msa0983670:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr6_4:26788117-26788136 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG - chr6_4:26787616-26787635 Msa0983670:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr6_4:26787638-26787657 Msa0983670:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr6_4:26788104-26788123 Msa0983670:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr6_4:26787616-26787635 Msa0983670:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr6_4:26787638-26787657 Msa0983670:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr6_4:26788104-26788123 Msa0983670:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + chr6_4:26787383-26787402 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr6_4:26787449-26787468 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr6_4:26787476-26787495 Msa0983670:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr6_4:26787477-26787496 Msa0983670:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + chr6_4:26787383-26787402 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr6_4:26787449-26787468 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr6_4:26787476-26787495 Msa0983670:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr6_4:26787477-26787496 Msa0983670:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG - chr6_4:26787364-26787383 Msa0983670:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr6_4:26787378-26787397 Msa0983670:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr6_4:26787385-26787404 Msa0983670:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr6_4:26787448-26787467 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr6_4:26787478-26787497 Msa0983670:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr6_4:26787976-26787995 Msa0983670:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - chr6_4:26787364-26787383 Msa0983670:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr6_4:26787378-26787397 Msa0983670:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr6_4:26787385-26787404 Msa0983670:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr6_4:26787448-26787467 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr6_4:26787478-26787497 Msa0983670:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr6_4:26787976-26787995 Msa0983670:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr6_4:26787395-26787414 Msa0983670:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr6_4:26787978-26787997 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr6_4:26787975-26787994 Msa0983670:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr6_4:26787395-26787414 Msa0983670:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr6_4:26787978-26787997 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr6_4:26787975-26787994 Msa0983670:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26787305 26788188 26787305 ID=Msa0983670;Name=Msa0983670
chr6_4 mRNA 26787305 26788188 26787305 ID=Msa0983670-mRNA-1;Parent=Msa0983670;Name=Msa0983670-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|105
chr6_4 exon 26788056 26788188 26788056 ID=Msa0983670-mRNA-1:exon:7580;Parent=Msa0983670-mRNA-1
chr6_4 exon 26787305 26787489 26787305 ID=Msa0983670-mRNA-1:exon:7579;Parent=Msa0983670-mRNA-1
chr6_4 CDS 26788056 26788188 26788056 ID=Msa0983670-mRNA-1:cds;Parent=Msa0983670-mRNA-1
chr6_4 CDS 26787305 26787489 26787305 ID=Msa0983670-mRNA-1:cds;Parent=Msa0983670-mRNA-1
Gene Sequence

>Msa0983670

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0983670

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*