AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0924820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924820 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0924820 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0924820 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0924820 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0924820 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0924820 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0924820 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0924820 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0924820 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924820 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0924820 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 6_2:-67447232 Msa0924820:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 6_2:-67447188 Msa0924820:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 6_2:+67447092 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 6_2:-67447863 Msa0924820:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 6_2:-67447187 Msa0924820:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 6_2:-67447846 Msa0924820:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 6_2:-67447133 Msa0924820:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 6_2:-67447856 Msa0924820:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 6_2:+67447093 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 6_2:+67447123 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 6_2:-67447877 Msa0924820:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 6_2:+67447861 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 6_2:-67447186 Msa0924820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG + chr6_2:67447233-67447252 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr6_2:67447357-67447376 Msa0924820:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr6_2:67447451-67447470 None:intergenic 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr6_2:67447233-67447252 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr6_2:67447357-67447376 Msa0924820:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr6_2:67447451-67447470 None:intergenic 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr6_2:67447081-67447100 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr6_2:67447080-67447099 Msa0924820:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr6_2:67447232-67447251 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr6_2:67447234-67447253 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr6_2:67447232-67447251 Msa0924820:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr6_2:67447287-67447306 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr6_2:67447360-67447379 Msa0924820:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr6_2:67447366-67447385 Msa0924820:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr6_2:67447482-67447501 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr6_2:67447775-67447794 Msa0924820:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr6_2:67447811-67447830 Msa0924820:intron 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr6_2:67447081-67447100 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr6_2:67447080-67447099 Msa0924820:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr6_2:67447232-67447251 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr6_2:67447234-67447253 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr6_2:67447232-67447251 Msa0924820:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr6_2:67447287-67447306 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr6_2:67447360-67447379 Msa0924820:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr6_2:67447366-67447385 Msa0924820:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr6_2:67447482-67447501 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr6_2:67447775-67447794 Msa0924820:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr6_2:67447811-67447830 Msa0924820:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr6_2:67447304-67447323 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr6_2:67447305-67447324 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr6_2:67447486-67447505 Msa0924820:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr6_2:67447487-67447506 Msa0924820:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr6_2:67447729-67447748 Msa0924820:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr6_2:67447304-67447323 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr6_2:67447305-67447324 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr6_2:67447486-67447505 Msa0924820:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr6_2:67447487-67447506 Msa0924820:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr6_2:67447729-67447748 Msa0924820:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - chr6_2:67447083-67447102 Msa0924820:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr6_2:67447121-67447140 Msa0924820:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr6_2:67447193-67447212 Msa0924820:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr6_2:67447237-67447256 Msa0924820:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr6_2:67447392-67447411 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr6_2:67447466-67447485 Msa0924820:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr6_2:67447490-67447509 Msa0924820:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr6_2:67447732-67447751 Msa0924820:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr6_2:67447765-67447784 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr6_2:67447819-67447838 Msa0924820:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr6_2:67447820-67447839 Msa0924820:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr6_2:67447821-67447840 Msa0924820:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr6_2:67447917-67447936 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr6_2:67447918-67447937 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - chr6_2:67447083-67447102 Msa0924820:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr6_2:67447121-67447140 Msa0924820:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr6_2:67447193-67447212 Msa0924820:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr6_2:67447237-67447256 Msa0924820:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr6_2:67447392-67447411 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr6_2:67447466-67447485 Msa0924820:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr6_2:67447490-67447509 Msa0924820:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr6_2:67447732-67447751 Msa0924820:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr6_2:67447765-67447784 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr6_2:67447819-67447838 Msa0924820:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr6_2:67447820-67447839 Msa0924820:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr6_2:67447821-67447840 Msa0924820:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr6_2:67447917-67447936 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr6_2:67447918-67447937 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr6_2:67447395-67447414 Msa0924820:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr6_2:67447415-67447434 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr6_2:67447416-67447435 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr6_2:67447491-67447510 Msa0924820:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr6_2:67447690-67447709 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr6_2:67447739-67447758 Msa0924820:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr6_2:67447887-67447906 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr6_2:67447395-67447414 Msa0924820:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr6_2:67447415-67447434 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr6_2:67447416-67447435 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr6_2:67447491-67447510 Msa0924820:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr6_2:67447690-67447709 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr6_2:67447739-67447758 Msa0924820:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr6_2:67447887-67447906 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG - chr6_2:67447382-67447401 Msa0924820:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr6_2:67447404-67447423 Msa0924820:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr6_2:67447874-67447893 Msa0924820:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr6_2:67447382-67447401 Msa0924820:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr6_2:67447404-67447423 Msa0924820:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr6_2:67447874-67447893 Msa0924820:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + chr6_2:67447149-67447168 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr6_2:67447215-67447234 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr6_2:67447242-67447261 Msa0924820:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr6_2:67447243-67447262 Msa0924820:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + chr6_2:67447149-67447168 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr6_2:67447215-67447234 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr6_2:67447242-67447261 Msa0924820:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr6_2:67447243-67447262 Msa0924820:intron 45.0%
!! ACTATATAAAATTAATTAAT+TGG - chr6_2:67447611-67447630 Msa0924820:intron 5.0%
!! ACTATATAAAATTAATTAAT+TGG - chr6_2:67447611-67447630 Msa0924820:intron 5.0%
ATATCTCTCTGTGGCACCAG+TGG - chr6_2:67447130-67447149 Msa0924820:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr6_2:67447144-67447163 Msa0924820:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr6_2:67447151-67447170 Msa0924820:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr6_2:67447214-67447233 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr6_2:67447244-67447263 Msa0924820:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr6_2:67447746-67447765 Msa0924820:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - chr6_2:67447130-67447149 Msa0924820:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr6_2:67447144-67447163 Msa0924820:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr6_2:67447151-67447170 Msa0924820:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr6_2:67447214-67447233 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr6_2:67447244-67447263 Msa0924820:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr6_2:67447746-67447765 Msa0924820:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr6_2:67447161-67447180 Msa0924820:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr6_2:67447748-67447767 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr6_2:67447745-67447764 Msa0924820:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr6_2:67447161-67447180 Msa0924820:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr6_2:67447748-67447767 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr6_2:67447745-67447764 Msa0924820:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 67447071 67447958 67447071 ID=Msa0924820;Name=Msa0924820
chr6_2 mRNA 67447071 67447958 67447071 ID=Msa0924820-mRNA-1;Parent=Msa0924820;Name=Msa0924820-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|105
chr6_2 exon 67447826 67447958 67447826 ID=Msa0924820-mRNA-1:exon:10680;Parent=Msa0924820-mRNA-1
chr6_2 exon 67447071 67447255 67447071 ID=Msa0924820-mRNA-1:exon:10679;Parent=Msa0924820-mRNA-1
chr6_2 CDS 67447826 67447958 67447826 ID=Msa0924820-mRNA-1:cds;Parent=Msa0924820-mRNA-1
chr6_2 CDS 67447071 67447255 67447071 ID=Msa0924820-mRNA-1:cds;Parent=Msa0924820-mRNA-1
Gene Sequence

>Msa0924820

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0924820

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*