AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0398620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398620 MtrunA17_CPg0492961 95.420 131 4 1 6 134 52 182 6.45e-85 246
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398620 ATCG00130.1 80.469 128 23 1 6 131 54 181 2.82e-68 204

Find 20 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTAATATTGATATGTTT+GGG 0.275522 3_2:+20512086 Msa0398620:CDS
GGTGCTAATATTGATATGTT+TGG 0.316362 3_2:+20512085 Msa0398620:CDS
TAATTCAATTTATACAACTT+TGG 0.388293 3_2:+20511910 Msa0398620:CDS
GCGGATCGGTTTCGAGTGAA+TGG 0.446998 3_2:+20511851 Msa0398620:CDS
TTCAAAATTCAGAAGAACTA+CGG 0.449969 3_2:+20511774 Msa0398620:CDS
GGAAGCAGCCGTTGAACAGC+TGG 0.450450 3_2:+20511796 Msa0398620:CDS
ACAAGCATTACAAGGAGCTC+TGG 0.452163 3_2:+20512015 Msa0398620:CDS
TCAAAATTCAGAAGAACTAC+GGG 0.510719 3_2:+20511775 Msa0398620:CDS
TGCTTTCTCCAGCTGTTCAA+CGG 0.514299 3_2:-20511804 None:intergenic
AGCTGGAGAAAGCACGGGTA+AGG 0.516157 3_2:+20511813 Msa0398620:CDS
CAAGCATTACAAGGAGCTCT+GGG 0.568354 3_2:+20512016 Msa0398620:CDS
AAGTAGAAACGGAAGCGGAT+CGG 0.570689 3_2:+20511837 Msa0398620:CDS
TTGAACAGCTGGAGAAAGCA+CGG 0.583807 3_2:+20511807 Msa0398620:CDS
TGCTAATATTGATATGTTTG+GGG 0.586102 3_2:+20512087 Msa0398620:CDS
CGATTAATCAAGTGCAACAG+AGG 0.596922 3_2:+20511984 Msa0398620:CDS
ACGGGTAAGGAAAGTAGAAA+CGG 0.601462 3_2:+20511826 Msa0398620:CDS
GAGTTACATTTACGTACGAT+CGG 0.617220 3_2:+20512064 Msa0398620:CDS
TGAACAGCTGGAGAAAGCAC+GGG 0.617623 3_2:+20511808 Msa0398620:CDS
AAGGAAAGTAGAAACGGAAG+CGG 0.641936 3_2:+20511832 Msa0398620:CDS
GATTAATCAAGTGCAACAGA+GGG 0.644246 3_2:+20511985 Msa0398620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATCTTAAATTACAAATTA+AGG + chr3_2:20509645-20509664 Msa0398620:intron 15.0%
!! TCTATTGTGTATAAAATTTA+TGG - chr3_2:20509695-20509714 None:intergenic 15.0%
!! CTAAAAAATTCATAATTATC+CGG - chr3_2:20509943-20509962 None:intergenic 15.0%
!!! TTTTTAGATTCTAAAAAATC+CGG + chr3_2:20509956-20509975 Msa0398620:intron 15.0%
!! ATCATTATCAGTATTAAAAA+AGG + chr3_2:20510567-20510586 Msa0398620:intron 15.0%
!! ATAAATTAATCAGAATTCTT+TGG + chr3_2:20511447-20511466 Msa0398620:intron 15.0%
!! CAATACAAGATTAAAATATT+TGG - chr3_2:20511529-20511548 None:intergenic 15.0%
!!! CAAATATTTTAATCTTGTAT+TGG + chr3_2:20511527-20511546 Msa0398620:intron 15.0%
!! TAATTCAATTTATACAACTT+TGG + chr3_2:20511910-20511929 Msa0398620:CDS 15.0%
!! AATTACAAATTAAGGTGATT+CGG + chr3_2:20509653-20509672 Msa0398620:intron 20.0%
!! ATAAAATTTATGGTTTCGAT+TGG - chr3_2:20509685-20509704 None:intergenic 20.0%
!!! CTAATAAGTTGTTTTATAGT+TGG + chr3_2:20509870-20509889 Msa0398620:intron 20.0%
!! GTCATATATATATAATGAAC+TGG + chr3_2:20509901-20509920 Msa0398620:intron 20.0%
!!! AATTAAAAAGTATTCCTGAA+AGG - chr3_2:20510062-20510081 None:intergenic 20.0%
!! ATTCTGATTAATTTATACGT+AGG - chr3_2:20511443-20511462 None:intergenic 20.0%
!! TACAAGATTAAAATATTTGG+AGG - chr3_2:20511526-20511545 None:intergenic 20.0%
! AAATTCATAATTATCCGGTT+AGG - chr3_2:20509938-20509957 None:intergenic 25.0%
! AATAGCAAATCCTGTATTTA+TGG + chr3_2:20510030-20510049 Msa0398620:intron 25.0%
! ATAGCAAATCCTGTATTTAT+GGG + chr3_2:20510031-20510050 Msa0398620:intron 25.0%
!! CAAAGCATTATATACTCAAA+AGG - chr3_2:20510102-20510121 None:intergenic 25.0%
!!! ACTTTTGAGTAATCCTTTTT+TGG + chr3_2:20510150-20510169 Msa0398620:intron 25.0%
! GCAGTATTCATGAATAATAA+AGG - chr3_2:20510195-20510214 None:intergenic 25.0%
! AATATTTATGCACAAAGAAC+AGG - chr3_2:20511293-20511312 None:intergenic 25.0%
! TTTGCTGACAATTATTTGTT+TGG + chr3_2:20511490-20511509 Msa0398620:intron 25.0%
! TGTTTAGAAATAGAGAAAAG+AGG + chr3_2:20511565-20511584 Msa0398620:intron 25.0%
! CTCATTCGATAATAAAGATA+GGG + chr3_2:20511593-20511612 Msa0398620:intron 25.0%
! CATGAATCTTTCAATTCATT+TGG - chr3_2:20511649-20511668 None:intergenic 25.0%
! TGAATTGAAAGATTCATGTT+TGG + chr3_2:20511651-20511670 Msa0398620:intron 25.0%
! TTCAAAATTCAGAAGAACTA+CGG + chr3_2:20511774-20511793 Msa0398620:CDS 25.0%
!! GATTCATTTTGAACAACAAA+GGG + chr3_2:20511961-20511980 Msa0398620:CDS 25.0%
! GTGCTAATATTGATATGTTT+GGG + chr3_2:20512086-20512105 Msa0398620:CDS 25.0%
! TGCTAATATTGATATGTTTG+GGG + chr3_2:20512087-20512106 Msa0398620:CDS 25.0%
! GATTTTGTTACATTTCGGAT+TGG + chr3_2:20509834-20509853 Msa0398620:intron 30.0%
!! AAGTTGTTTTATAGTTGGCA+TGG + chr3_2:20509875-20509894 Msa0398620:intron 30.0%
! TAGATTCTAAAAAATCCGGT+TGG + chr3_2:20509960-20509979 Msa0398620:intron 30.0%
ACTTTAATTGTCTTACCAAC+CGG - chr3_2:20509978-20509997 None:intergenic 30.0%
! AAGGTATTACCCATAAATAC+AGG - chr3_2:20510043-20510062 None:intergenic 30.0%
ATTTATGGGTAATACCTTTC+AGG + chr3_2:20510045-20510064 Msa0398620:intron 30.0%
!! TGCTTTGTTTAGAATGCAAA+TGG + chr3_2:20510115-20510134 Msa0398620:intron 30.0%
! TTGTTTAGAATGCAAATGGT+TGG + chr3_2:20510119-20510138 Msa0398620:intron 30.0%
! TGTTTAGAATGCAAATGGTT+GGG + chr3_2:20510120-20510139 Msa0398620:intron 30.0%
!!! TGAGTAATCCTTTTTTGGAA+AGG + chr3_2:20510155-20510174 Msa0398620:intron 30.0%
!!! GAGTAATCCTTTTTTGGAAA+GGG + chr3_2:20510156-20510175 Msa0398620:intron 30.0%
CAAATAACCCTTTCCAAAAA+AGG - chr3_2:20510166-20510185 None:intergenic 30.0%
GATATACAAAGACATGGAAA+GGG - chr3_2:20511255-20511274 None:intergenic 30.0%
AGATATACAAAGACATGGAA+AGG - chr3_2:20511256-20511275 None:intergenic 30.0%
TCAATTCCGCATTGTATAAA+AGG - chr3_2:20511417-20511436 None:intergenic 30.0%
AGAAATAGAGAAAAGAGGAT+AGG + chr3_2:20511570-20511589 Msa0398620:intron 30.0%
GCTCATTCGATAATAAAGAT+AGG + chr3_2:20511592-20511611 Msa0398620:intron 30.0%
AGATAGGGAAATTTCCTATA+CGG + chr3_2:20511608-20511627 Msa0398620:intron 30.0%
TGAAAGATTCATGTTTGGTT+CGG + chr3_2:20511656-20511675 Msa0398620:intron 30.0%
TCAAAATTCAGAAGAACTAC+GGG + chr3_2:20511775-20511794 Msa0398620:CDS 30.0%
! CAATTTTTCTCGTTCTATCT+CGG - chr3_2:20511882-20511901 None:intergenic 30.0%
! CGATTCATTTTGAACAACAA+AGG + chr3_2:20511960-20511979 Msa0398620:CDS 30.0%
! GTTTTACAACAAGCATTACA+AGG + chr3_2:20512007-20512026 Msa0398620:CDS 30.0%
GGTGCTAATATTGATATGTT+TGG + chr3_2:20512085-20512104 Msa0398620:CDS 30.0%
! CGGGAGATTTTGTTACATTT+CGG + chr3_2:20509829-20509848 Msa0398620:intron 35.0%
TGGTTTAGATCAATCCTAAC+CGG + chr3_2:20509921-20509940 Msa0398620:intron 35.0%
! GTTTAGAATGCAAATGGTTG+GGG + chr3_2:20510121-20510140 Msa0398620:intron 35.0%
! TTTAGAATGCAAATGGTTGG+GGG + chr3_2:20510122-20510141 Msa0398620:intron 35.0%
ATTCCGCATTGTATAAAAGG+AGG - chr3_2:20511414-20511433 None:intergenic 35.0%
! GAAAGATTCATGTTTGGTTC+GGG + chr3_2:20511657-20511676 Msa0398620:intron 35.0%
GATTAATCAAGTGCAACAGA+GGG + chr3_2:20511985-20512004 Msa0398620:CDS 35.0%
GAGTTACATTTACGTACGAT+CGG + chr3_2:20512064-20512083 Msa0398620:CDS 35.0%
TAACAAAATCTCCCGCTCTT+CGG - chr3_2:20509824-20509843 None:intergenic 40.0%
AAAAGCAAACCCCATAGAGA+AGG + chr3_2:20510702-20510721 Msa0398620:intron 40.0%
AAAGCAAACCCCATAGAGAA+GGG + chr3_2:20510703-20510722 Msa0398620:intron 40.0%
CTGGCAGATATACAAAGACA+TGG - chr3_2:20511261-20511280 None:intergenic 40.0%
CATGTCTTTGTATATCTGCC+AGG + chr3_2:20511259-20511278 Msa0398620:intron 40.0%
! CCTGCTACTTCTTTGTATGA+GGG - chr3_2:20511377-20511396 None:intergenic 40.0%
! ACCTGCTACTTCTTTGTATG+AGG - chr3_2:20511378-20511397 None:intergenic 40.0%
! ACGCCTCCTTTTATACAATG+CGG + chr3_2:20511408-20511427 Msa0398620:intron 40.0%
TCTCACACTCAATTCCGTAT+AGG - chr3_2:20511625-20511644 None:intergenic 40.0%
ACGGGTAAGGAAAGTAGAAA+CGG + chr3_2:20511826-20511845 Msa0398620:CDS 40.0%
AAGGAAAGTAGAAACGGAAG+CGG + chr3_2:20511832-20511851 Msa0398620:CDS 40.0%
CGATTAATCAAGTGCAACAG+AGG + chr3_2:20511984-20512003 Msa0398620:CDS 40.0%
ACACCCTAGTACATGTTCCT+CGG + chr3_2:20509784-20509803 Msa0398620:intron 45.0%
AACAAAATCTCCCGCTCTTC+GGG - chr3_2:20509823-20509842 None:intergenic 45.0%
AAGCAAACCCCATAGAGAAG+GGG + chr3_2:20510704-20510723 Msa0398620:intron 45.0%
GACCGTATCAACAATTCCGT+GGG + chr3_2:20511199-20511218 Msa0398620:intron 45.0%
AGCCCACGGAATTGTTGATA+CGG - chr3_2:20511204-20511223 None:intergenic 45.0%
AAAGAACAGGCAAAGCTTCC+TGG - chr3_2:20511280-20511299 None:intergenic 45.0%
ACAAGCAGGAGAATGTATGC+TGG - chr3_2:20511354-20511373 None:intergenic 45.0%
TCTTTGTATGAGGGACAAGC+AGG - chr3_2:20511368-20511387 None:intergenic 45.0%
CCCTCATACAAAGAAGTAGC+AGG + chr3_2:20511374-20511393 Msa0398620:intron 45.0%
TGCTTTCTCCAGCTGTTCAA+CGG - chr3_2:20511807-20511826 None:intergenic 45.0%
TTGAACAGCTGGAGAAAGCA+CGG + chr3_2:20511807-20511826 Msa0398620:CDS 45.0%
AAGTAGAAACGGAAGCGGAT+CGG + chr3_2:20511837-20511856 Msa0398620:CDS 45.0%
ACAAGCATTACAAGGAGCTC+TGG + chr3_2:20512015-20512034 Msa0398620:CDS 45.0%
CAAGCATTACAAGGAGCTCT+GGG + chr3_2:20512016-20512035 Msa0398620:CDS 45.0%
ACAAAATCTCCCGCTCTTCG+GGG - chr3_2:20509822-20509841 None:intergenic 50.0%
AGCAAACCCCATAGAGAAGG+GGG + chr3_2:20510705-20510724 Msa0398620:intron 50.0%
CGACCGTATCAACAATTCCG+TGG + chr3_2:20511198-20511217 Msa0398620:intron 50.0%
! TGAACAGCTGGAGAAAGCAC+GGG + chr3_2:20511808-20511827 Msa0398620:CDS 50.0%
GCGCCGAGGAACATGTACTA+GGG - chr3_2:20509790-20509809 None:intergenic 55.0%
! AGCGCCGAGGAACATGTACT+AGG - chr3_2:20509791-20509810 None:intergenic 55.0%
! TACATGTTCCTCGGCGCTGA+GGG + chr3_2:20509793-20509812 Msa0398620:intron 55.0%
AACCCCATAGAGAAGGGGGA+AGG + chr3_2:20510709-20510728 Msa0398620:intron 55.0%
ACCCCATAGAGAAGGGGGAA+GGG + chr3_2:20510710-20510729 Msa0398620:intron 55.0%
TCCCCTTCCCCCTTCTCTAT+GGG - chr3_2:20510715-20510734 None:intergenic 55.0%
ATAGAGAAGGGGGAAGGGGA+GGG + chr3_2:20510715-20510734 Msa0398620:intron 55.0%
GCAGAAGAAGCTCAAGCCCA+CGG - chr3_2:20511218-20511237 None:intergenic 55.0%
! AGCTGGAGAAAGCACGGGTA+AGG + chr3_2:20511813-20511832 Msa0398620:CDS 55.0%
! GCGGATCGGTTTCGAGTGAA+TGG + chr3_2:20511851-20511870 Msa0398620:CDS 55.0%
GTACATGTTCCTCGGCGCTG+AGG + chr3_2:20509792-20509811 Msa0398620:intron 60.0%
CCCCTTCCCCCTTCTCTATG+GGG - chr3_2:20510714-20510733 None:intergenic 60.0%
CCCCATAGAGAAGGGGGAAG+GGG + chr3_2:20510711-20510730 Msa0398620:intron 60.0%
CTCCCCTTCCCCCTTCTCTA+TGG - chr3_2:20510716-20510735 None:intergenic 60.0%
CATAGAGAAGGGGGAAGGGG+AGG + chr3_2:20510714-20510733 Msa0398620:intron 60.0%
TAGAGAAGGGGGAAGGGGAG+GGG + chr3_2:20510716-20510735 Msa0398620:intron 60.0%
GGAAGCAGCCGTTGAACAGC+TGG + chr3_2:20511796-20511815 Msa0398620:CDS 60.0%
!! CTGAGGGCATCCCCGAAGAG+CGG + chr3_2:20509809-20509828 Msa0398620:intron 65.0%
!! TGAGGGCATCCCCGAAGAGC+GGG + chr3_2:20509810-20509829 Msa0398620:intron 65.0%
AGAGAAGGGGGAAGGGGAGG+GGG + chr3_2:20510717-20510736 Msa0398620:intron 65.0%
! CGGGGATGCCCTCAGCGCCG+AGG - chr3_2:20509804-20509823 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 20509630 20512132 20509630 ID=Msa0398620;Name=Msa0398620
chr3_2 mRNA 20509630 20512132 20509630 ID=Msa0398620-mRNA-1;Parent=Msa0398620;Name=Msa0398620-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|0.33|1|1|3|0|134
chr3_2 exon 20509630 20509637 20509630 ID=Msa0398620-mRNA-1:exon:4458;Parent=Msa0398620-mRNA-1
chr3_2 exon 20509889 20509896 20509889 ID=Msa0398620-mRNA-1:exon:4459;Parent=Msa0398620-mRNA-1
chr3_2 exon 20511744 20512132 20511744 ID=Msa0398620-mRNA-1:exon:4460;Parent=Msa0398620-mRNA-1
chr3_2 CDS 20509630 20509637 20509630 ID=Msa0398620-mRNA-1:cds;Parent=Msa0398620-mRNA-1
chr3_2 CDS 20509889 20509896 20509889 ID=Msa0398620-mRNA-1:cds;Parent=Msa0398620-mRNA-1
chr3_2 CDS 20511744 20512132 20511744 ID=Msa0398620-mRNA-1:cds;Parent=Msa0398620-mRNA-1
Gene Sequence

>Msa0398620

ATGGGAAATTGGCATGATAATCGAAAACAGAGAATCTTGAGAACTATTCAAAATTCAGAAGAACTACGGGAAGCAGCCGTTGAACAGCTGGAGAAAGCACGGGTAAGGAAAGTAGAAACGGAAGCGGATCGGTTTCGAGTGAATGGTTATTCCGAGATAGAACGAGAAAAATTGAATTTAATTAATTCAATTTATACAACTTTGGAACAATTCGAAAATTACAAAAATGAAACGATTCATTTTGAACAACAAAGGGCGATTAATCAAGTGCAACAGAGGGTTTTACAACAAGCATTACAAGGAGCTCTGGGAACTCTGAATAGTTGCTTAAACAACGAGTTACATTTACGTACGATCGGTGCTAATATTGATATGTTTGGGGCGATGAAAGAAAAAAATAACTGA

Protein sequence

>Msa0398620

MGNWHDNRKQRILRTIQNSEELREAAVEQLEKARVRKVETEADRFRVNGYSEIEREKLNLINSIYTTLEQFENYKNETIHFEQQRAINQVQQRVLQQALQGALGTLNSCLNNELHLRTIGANIDMFGAMKEKNN*