AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268220 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0268220 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0268220 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0268220 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0268220 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268220 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268220 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268220 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268220 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268220 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0268220 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 2_2:+70130179 Msa0268220:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 2_2:+70130223 Msa0268220:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 2_2:-70130319 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 2_2:+70129552 Msa0268220:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 2_2:+70130224 Msa0268220:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 2_2:+70129569 Msa0268220:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 2_2:+70130278 Msa0268220:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 2_2:+70129559 Msa0268220:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 2_2:-70130318 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 2_2:-70130288 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 2_2:+70129538 Msa0268220:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 2_2:-70129554 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 2_2:+70130225 Msa0268220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG - chr2_2:70129641-70129660 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr2_2:70129765-70129784 Msa0268220:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr2_2:70129859-70129878 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr2_2:70130015-70130034 Msa0268220:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG - chr2_2:70129641-70129660 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr2_2:70129765-70129784 Msa0268220:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr2_2:70129859-70129878 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr2_2:70130015-70130034 Msa0268220:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr2_2:70129489-70129508 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr2_2:70129488-70129507 Msa0268220:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr2_2:70129640-70129659 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr2_2:70129642-70129661 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr2_2:70129640-70129659 Msa0268220:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr2_2:70129695-70129714 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr2_2:70129768-70129787 Msa0268220:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr2_2:70129774-70129793 Msa0268220:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr2_2:70129890-70129909 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr2_2:70130179-70130198 Msa0268220:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr2_2:70130215-70130234 Msa0268220:CDS 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr2_2:70129489-70129508 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr2_2:70129488-70129507 Msa0268220:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr2_2:70129640-70129659 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr2_2:70129642-70129661 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr2_2:70129640-70129659 Msa0268220:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr2_2:70129695-70129714 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr2_2:70129768-70129787 Msa0268220:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr2_2:70129774-70129793 Msa0268220:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr2_2:70129890-70129909 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr2_2:70130179-70130198 Msa0268220:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr2_2:70130215-70130234 Msa0268220:CDS 20.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr2_2:70129712-70129731 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr2_2:70129713-70129732 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr2_2:70129894-70129913 Msa0268220:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr2_2:70129895-70129914 Msa0268220:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr2_2:70130133-70130152 Msa0268220:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr2_2:70129712-70129731 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr2_2:70129713-70129732 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr2_2:70129894-70129913 Msa0268220:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr2_2:70129895-70129914 Msa0268220:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr2_2:70130133-70130152 Msa0268220:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG + chr2_2:70129491-70129510 Msa0268220:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr2_2:70129529-70129548 Msa0268220:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr2_2:70129601-70129620 Msa0268220:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr2_2:70129645-70129664 Msa0268220:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr2_2:70129800-70129819 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr2_2:70129874-70129893 Msa0268220:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr2_2:70129898-70129917 Msa0268220:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr2_2:70130136-70130155 Msa0268220:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr2_2:70130169-70130188 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr2_2:70130223-70130242 Msa0268220:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr2_2:70130224-70130243 Msa0268220:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr2_2:70130225-70130244 Msa0268220:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr2_2:70130321-70130340 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr2_2:70130322-70130341 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG + chr2_2:70129491-70129510 Msa0268220:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr2_2:70129529-70129548 Msa0268220:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr2_2:70129601-70129620 Msa0268220:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr2_2:70129645-70129664 Msa0268220:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr2_2:70129800-70129819 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr2_2:70129874-70129893 Msa0268220:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr2_2:70129898-70129917 Msa0268220:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr2_2:70130136-70130155 Msa0268220:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr2_2:70130169-70130188 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr2_2:70130223-70130242 Msa0268220:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr2_2:70130224-70130243 Msa0268220:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr2_2:70130225-70130244 Msa0268220:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr2_2:70130321-70130340 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr2_2:70130322-70130341 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr2_2:70129803-70129822 Msa0268220:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr2_2:70129823-70129842 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr2_2:70129824-70129843 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr2_2:70129899-70129918 Msa0268220:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr2_2:70130094-70130113 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr2_2:70130143-70130162 Msa0268220:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr2_2:70130291-70130310 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr2_2:70129803-70129822 Msa0268220:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr2_2:70129823-70129842 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr2_2:70129824-70129843 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr2_2:70129899-70129918 Msa0268220:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr2_2:70130094-70130113 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr2_2:70130143-70130162 Msa0268220:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr2_2:70130291-70130310 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG + chr2_2:70129790-70129809 Msa0268220:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr2_2:70129812-70129831 Msa0268220:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr2_2:70130278-70130297 Msa0268220:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr2_2:70129790-70129809 Msa0268220:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr2_2:70129812-70129831 Msa0268220:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr2_2:70130278-70130297 Msa0268220:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG - chr2_2:70129557-70129576 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr2_2:70129623-70129642 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr2_2:70129650-70129669 Msa0268220:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr2_2:70129651-70129670 Msa0268220:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG - chr2_2:70129557-70129576 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr2_2:70129623-70129642 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr2_2:70129650-70129669 Msa0268220:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr2_2:70129651-70129670 Msa0268220:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG + chr2_2:70129538-70129557 Msa0268220:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr2_2:70129552-70129571 Msa0268220:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr2_2:70129559-70129578 Msa0268220:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr2_2:70129622-70129641 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr2_2:70129652-70129671 Msa0268220:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr2_2:70130150-70130169 Msa0268220:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG + chr2_2:70129538-70129557 Msa0268220:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr2_2:70129552-70129571 Msa0268220:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr2_2:70129559-70129578 Msa0268220:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr2_2:70129622-70129641 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr2_2:70129652-70129671 Msa0268220:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr2_2:70130150-70130169 Msa0268220:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr2_2:70129569-70129588 Msa0268220:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr2_2:70130152-70130171 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr2_2:70130149-70130168 Msa0268220:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr2_2:70129569-70129588 Msa0268220:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr2_2:70130152-70130171 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr2_2:70130149-70130168 Msa0268220:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70129479 70130362 70129479 ID=Msa0268220;Name=Msa0268220
chr2_2 mRNA 70129479 70130362 70129479 ID=Msa0268220-mRNA-1;Parent=Msa0268220;Name=Msa0268220-mRNA-1;_AED=0.45;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|105
chr2_2 exon 70129479 70129611 70129479 ID=Msa0268220-mRNA-1:exon:22515;Parent=Msa0268220-mRNA-1
chr2_2 exon 70130178 70130362 70130178 ID=Msa0268220-mRNA-1:exon:22516;Parent=Msa0268220-mRNA-1
chr2_2 CDS 70129479 70129611 70129479 ID=Msa0268220-mRNA-1:cds;Parent=Msa0268220-mRNA-1
chr2_2 CDS 70130178 70130362 70130178 ID=Msa0268220-mRNA-1:cds;Parent=Msa0268220-mRNA-1
Gene Sequence

>Msa0268220

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0268220

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*