AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086330 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa1086330 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa1086330 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa1086330 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa1086330 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1086330 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1086330 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1086330 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa1086330 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086330 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa1086330 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 7_2:+87315678 Msa1086330:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 7_2:+87315722 Msa1086330:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 7_2:-87315818 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 7_2:+87315051 Msa1086330:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 7_2:+87315723 Msa1086330:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 7_2:+87315068 Msa1086330:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 7_2:+87315777 Msa1086330:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 7_2:+87315058 Msa1086330:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 7_2:-87315817 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 7_2:-87315787 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 7_2:+87315037 Msa1086330:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 7_2:-87315053 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 7_2:+87315724 Msa1086330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG - chr7_2:87315140-87315159 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr7_2:87315264-87315283 Msa1086330:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr7_2:87315358-87315377 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr7_2:87315514-87315533 Msa1086330:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG - chr7_2:87315140-87315159 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr7_2:87315264-87315283 Msa1086330:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr7_2:87315358-87315377 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr7_2:87315514-87315533 Msa1086330:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr7_2:87314988-87315007 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr7_2:87314987-87315006 Msa1086330:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr7_2:87315139-87315158 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr7_2:87315141-87315160 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr7_2:87315139-87315158 Msa1086330:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr7_2:87315194-87315213 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr7_2:87315267-87315286 Msa1086330:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr7_2:87315273-87315292 Msa1086330:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr7_2:87315389-87315408 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr7_2:87315678-87315697 Msa1086330:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr7_2:87315714-87315733 Msa1086330:CDS 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr7_2:87314988-87315007 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr7_2:87314987-87315006 Msa1086330:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr7_2:87315139-87315158 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr7_2:87315141-87315160 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr7_2:87315139-87315158 Msa1086330:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr7_2:87315194-87315213 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr7_2:87315267-87315286 Msa1086330:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr7_2:87315273-87315292 Msa1086330:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr7_2:87315389-87315408 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr7_2:87315678-87315697 Msa1086330:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr7_2:87315714-87315733 Msa1086330:CDS 20.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr7_2:87315211-87315230 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr7_2:87315212-87315231 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr7_2:87315393-87315412 Msa1086330:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr7_2:87315394-87315413 Msa1086330:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr7_2:87315632-87315651 Msa1086330:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr7_2:87315211-87315230 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr7_2:87315212-87315231 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr7_2:87315393-87315412 Msa1086330:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr7_2:87315394-87315413 Msa1086330:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr7_2:87315632-87315651 Msa1086330:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG + chr7_2:87314990-87315009 Msa1086330:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr7_2:87315028-87315047 Msa1086330:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr7_2:87315100-87315119 Msa1086330:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr7_2:87315144-87315163 Msa1086330:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr7_2:87315299-87315318 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr7_2:87315373-87315392 Msa1086330:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr7_2:87315397-87315416 Msa1086330:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr7_2:87315635-87315654 Msa1086330:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr7_2:87315668-87315687 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr7_2:87315722-87315741 Msa1086330:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr7_2:87315723-87315742 Msa1086330:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr7_2:87315724-87315743 Msa1086330:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr7_2:87315820-87315839 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr7_2:87315821-87315840 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG + chr7_2:87314990-87315009 Msa1086330:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr7_2:87315028-87315047 Msa1086330:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr7_2:87315100-87315119 Msa1086330:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr7_2:87315144-87315163 Msa1086330:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr7_2:87315299-87315318 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr7_2:87315373-87315392 Msa1086330:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr7_2:87315397-87315416 Msa1086330:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr7_2:87315635-87315654 Msa1086330:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr7_2:87315668-87315687 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr7_2:87315722-87315741 Msa1086330:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr7_2:87315723-87315742 Msa1086330:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr7_2:87315724-87315743 Msa1086330:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr7_2:87315820-87315839 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr7_2:87315821-87315840 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr7_2:87315302-87315321 Msa1086330:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr7_2:87315322-87315341 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr7_2:87315323-87315342 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr7_2:87315398-87315417 Msa1086330:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr7_2:87315593-87315612 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr7_2:87315642-87315661 Msa1086330:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr7_2:87315790-87315809 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr7_2:87315302-87315321 Msa1086330:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr7_2:87315322-87315341 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr7_2:87315323-87315342 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr7_2:87315398-87315417 Msa1086330:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr7_2:87315593-87315612 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr7_2:87315642-87315661 Msa1086330:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr7_2:87315790-87315809 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG + chr7_2:87315289-87315308 Msa1086330:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr7_2:87315311-87315330 Msa1086330:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr7_2:87315777-87315796 Msa1086330:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr7_2:87315289-87315308 Msa1086330:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr7_2:87315311-87315330 Msa1086330:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr7_2:87315777-87315796 Msa1086330:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG - chr7_2:87315056-87315075 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr7_2:87315122-87315141 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr7_2:87315149-87315168 Msa1086330:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr7_2:87315150-87315169 Msa1086330:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG - chr7_2:87315056-87315075 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr7_2:87315122-87315141 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr7_2:87315149-87315168 Msa1086330:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr7_2:87315150-87315169 Msa1086330:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG + chr7_2:87315037-87315056 Msa1086330:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr7_2:87315051-87315070 Msa1086330:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr7_2:87315058-87315077 Msa1086330:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr7_2:87315121-87315140 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr7_2:87315151-87315170 Msa1086330:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr7_2:87315649-87315668 Msa1086330:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG + chr7_2:87315037-87315056 Msa1086330:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr7_2:87315051-87315070 Msa1086330:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr7_2:87315058-87315077 Msa1086330:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr7_2:87315121-87315140 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr7_2:87315151-87315170 Msa1086330:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr7_2:87315649-87315668 Msa1086330:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr7_2:87315068-87315087 Msa1086330:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr7_2:87315651-87315670 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr7_2:87315648-87315667 Msa1086330:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr7_2:87315068-87315087 Msa1086330:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr7_2:87315651-87315670 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr7_2:87315648-87315667 Msa1086330:intron 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87314978 87315861 87314978 ID=Msa1086330;Name=Msa1086330
chr7_2 mRNA 87314978 87315861 87314978 ID=Msa1086330-mRNA-1;Parent=Msa1086330;Name=Msa1086330-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|105
chr7_2 exon 87314978 87315110 87314978 ID=Msa1086330-mRNA-1:exon:23775;Parent=Msa1086330-mRNA-1
chr7_2 exon 87315677 87315861 87315677 ID=Msa1086330-mRNA-1:exon:23776;Parent=Msa1086330-mRNA-1
chr7_2 CDS 87314978 87315110 87314978 ID=Msa1086330-mRNA-1:cds;Parent=Msa1086330-mRNA-1
chr7_2 CDS 87315677 87315861 87315677 ID=Msa1086330-mRNA-1:cds;Parent=Msa1086330-mRNA-1
Gene Sequence

>Msa1086330

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa1086330

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*