AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0582570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582570 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0582570 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0582570 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0582570 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0582570 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0582570 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0582570 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0582570 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0582570 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582570 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0582570 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 4_2:+1073995 Msa0582570:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 4_2:+1074039 Msa0582570:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 4_2:-1074135 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 4_2:+1073368 Msa0582570:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 4_2:+1074040 Msa0582570:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 4_2:+1073385 Msa0582570:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 4_2:+1074094 Msa0582570:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 4_2:+1073375 Msa0582570:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 4_2:-1074134 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 4_2:-1074104 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 4_2:+1073354 Msa0582570:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 4_2:-1073370 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 4_2:+1074041 Msa0582570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG - chr4_2:1073457-1073476 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr4_2:1073581-1073600 Msa0582570:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr4_2:1073675-1073694 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr4_2:1073831-1073850 Msa0582570:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG - chr4_2:1073457-1073476 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr4_2:1073581-1073600 Msa0582570:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr4_2:1073675-1073694 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr4_2:1073831-1073850 Msa0582570:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr4_2:1073305-1073324 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr4_2:1073304-1073323 Msa0582570:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr4_2:1073456-1073475 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr4_2:1073458-1073477 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr4_2:1073456-1073475 Msa0582570:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr4_2:1073511-1073530 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr4_2:1073584-1073603 Msa0582570:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr4_2:1073590-1073609 Msa0582570:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr4_2:1073706-1073725 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr4_2:1073995-1074014 Msa0582570:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr4_2:1074031-1074050 Msa0582570:CDS 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG - chr4_2:1073305-1073324 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr4_2:1073304-1073323 Msa0582570:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr4_2:1073456-1073475 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr4_2:1073458-1073477 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr4_2:1073456-1073475 Msa0582570:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr4_2:1073511-1073530 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr4_2:1073584-1073603 Msa0582570:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr4_2:1073590-1073609 Msa0582570:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr4_2:1073706-1073725 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr4_2:1073995-1074014 Msa0582570:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr4_2:1074031-1074050 Msa0582570:CDS 20.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr4_2:1073528-1073547 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr4_2:1073529-1073548 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr4_2:1073710-1073729 Msa0582570:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr4_2:1073711-1073730 Msa0582570:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr4_2:1073949-1073968 Msa0582570:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr4_2:1073528-1073547 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr4_2:1073529-1073548 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr4_2:1073710-1073729 Msa0582570:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr4_2:1073711-1073730 Msa0582570:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr4_2:1073949-1073968 Msa0582570:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG + chr4_2:1073307-1073326 Msa0582570:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr4_2:1073345-1073364 Msa0582570:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr4_2:1073417-1073436 Msa0582570:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr4_2:1073461-1073480 Msa0582570:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr4_2:1073616-1073635 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr4_2:1073690-1073709 Msa0582570:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr4_2:1073714-1073733 Msa0582570:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr4_2:1073952-1073971 Msa0582570:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr4_2:1073985-1074004 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr4_2:1074039-1074058 Msa0582570:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr4_2:1074040-1074059 Msa0582570:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr4_2:1074041-1074060 Msa0582570:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr4_2:1074137-1074156 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr4_2:1074138-1074157 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG + chr4_2:1073307-1073326 Msa0582570:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr4_2:1073345-1073364 Msa0582570:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr4_2:1073417-1073436 Msa0582570:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr4_2:1073461-1073480 Msa0582570:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr4_2:1073616-1073635 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr4_2:1073690-1073709 Msa0582570:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr4_2:1073714-1073733 Msa0582570:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr4_2:1073952-1073971 Msa0582570:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr4_2:1073985-1074004 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr4_2:1074039-1074058 Msa0582570:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr4_2:1074040-1074059 Msa0582570:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr4_2:1074041-1074060 Msa0582570:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr4_2:1074137-1074156 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr4_2:1074138-1074157 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr4_2:1073619-1073638 Msa0582570:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr4_2:1073639-1073658 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr4_2:1073640-1073659 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr4_2:1073715-1073734 Msa0582570:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr4_2:1073910-1073929 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr4_2:1073959-1073978 Msa0582570:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr4_2:1074107-1074126 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr4_2:1073619-1073638 Msa0582570:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr4_2:1073639-1073658 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr4_2:1073640-1073659 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr4_2:1073715-1073734 Msa0582570:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr4_2:1073910-1073929 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr4_2:1073959-1073978 Msa0582570:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr4_2:1074107-1074126 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG + chr4_2:1073606-1073625 Msa0582570:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr4_2:1073628-1073647 Msa0582570:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr4_2:1074094-1074113 Msa0582570:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr4_2:1073606-1073625 Msa0582570:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr4_2:1073628-1073647 Msa0582570:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr4_2:1074094-1074113 Msa0582570:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG - chr4_2:1073373-1073392 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr4_2:1073439-1073458 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr4_2:1073466-1073485 Msa0582570:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr4_2:1073467-1073486 Msa0582570:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG - chr4_2:1073373-1073392 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr4_2:1073439-1073458 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr4_2:1073466-1073485 Msa0582570:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr4_2:1073467-1073486 Msa0582570:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG + chr4_2:1073354-1073373 Msa0582570:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr4_2:1073368-1073387 Msa0582570:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr4_2:1073375-1073394 Msa0582570:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr4_2:1073438-1073457 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr4_2:1073468-1073487 Msa0582570:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr4_2:1073966-1073985 Msa0582570:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG + chr4_2:1073354-1073373 Msa0582570:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr4_2:1073368-1073387 Msa0582570:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr4_2:1073375-1073394 Msa0582570:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr4_2:1073438-1073457 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr4_2:1073468-1073487 Msa0582570:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr4_2:1073966-1073985 Msa0582570:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr4_2:1073385-1073404 Msa0582570:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr4_2:1073968-1073987 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr4_2:1073965-1073984 Msa0582570:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr4_2:1073385-1073404 Msa0582570:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr4_2:1073968-1073987 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr4_2:1073965-1073984 Msa0582570:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 1073295 1074178 1073295 ID=Msa0582570;Name=Msa0582570
chr4_2 mRNA 1073295 1074178 1073295 ID=Msa0582570-mRNA-1;Parent=Msa0582570;Name=Msa0582570-mRNA-1;_AED=0.45;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|105
chr4_2 exon 1073295 1073427 1073295 ID=Msa0582570-mRNA-1:exon:214;Parent=Msa0582570-mRNA-1
chr4_2 exon 1073994 1074178 1073994 ID=Msa0582570-mRNA-1:exon:215;Parent=Msa0582570-mRNA-1
chr4_2 CDS 1073295 1073427 1073295 ID=Msa0582570-mRNA-1:cds;Parent=Msa0582570-mRNA-1
chr4_2 CDS 1073994 1074178 1073994 ID=Msa0582570-mRNA-1:cds;Parent=Msa0582570-mRNA-1
Gene Sequence

>Msa0582570

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0582570

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*